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This review summarizes over 280 studies published in 2019 related to the characterization, control, and management of urban stormwater runoff. A summary of quantity and quality concerns is provided in the first section of the review, serving as the foundation for the following sections which focus on the control and treatment of stormwater runoff. Finally, the impact of stormwater control devices at the watershed scale is discussed. Each section provides a self-contained overview of the 2019 literature, common themes, and future work. Several themes emerged from the 2019 literature including exploration of substrate amendments for improved water quality effluent from stormwater controls, the continued study of the role of vegetation in green infrastructure practices, and a call to action for the development of new models which generate reliable, computationally efficient results under the physical, chemical, biological, and social complexity of stormwater management. PRACTITIONER POINTS: Over 280 studies were published in 2019 related to the characterization, control, and treatment of urban stormwater. Studies on bioretention and general stormwater characteristics represented the two most common subtopics in 2019. Trends in 2019 included novel substrate amendments, studies on the role of vegetation, and advancements in computational models.

The Clean Water Act of 1972 was the impetus for stormwater management in the United States, followed by the need for many cities to comply with consent decrees associated with combined sewer overflows. With rapidly growing urban centers and the attendant increasing costs of managing stormwater with larger stormwater facilities, green stormwater infrastructure (GSI) was deemed a useful measure to distribute the management of stormwater across the landscape. The management of stormwater has evolved from simply removing it as quickly as it is generated in order to prevent flooding, to intentionally detaining stormwater on the landscape. Typically, low-frequency large events are detained in central stormwater holding facilities, while GSI is employed to manage smaller high-frequency events, slowing and treating stormwater on the landscape itself. Installing GSI close to the source of runoff production ensures that stormwater directed towards these facilities are small enough in volume, so as not to overwhelm these systems. Within these GSI systems, the natural assimilative capacity of soils and plants slows and breaks down many of the pollutants that are found in stormwater runoff. The requirement for a broad spatial distribution of GSI across the landscape necessitates an acceptance of these technologies, and the willingness of the managers of these urban landscapes to maintain these systems on a continual basis. The policies put in place to transfer the responsibility of stormwater management onto individual lot owners range from regulations imposed on those that develop the landscape for commercial and industrial purposes, to incentives offered to individual lot owners to install GSI practices for the first time on their properties. GSI is, however, not a silver bullet for all stormwater ills, and care has to be taken in how it is deployed in order not to exacerbate systemic environmental and racial inequities. A careful and considered adoption of GSI that includes the desires, values, and the needs of the community in conjunction with the environmental goals they are designed to address is critical.

As urbanization continues, water pollution is of increasing concern for human health and the environment. Water contaminants common in urban stormwater include nutrients, metals, suspended solids, pesticides, and pathogens. The search for an inexpensive and readily available material that can effectively remove common stormwater contaminants is ongoing. Studies have shown that woodchips are a promising material that can remove many different contaminants, including these common contaminants and emerging contaminants of concern. The type of wood and shape of woodchips can impact the removal efficiencies of different contaminants due to different partitioning coefficients and capillary action. This review compiles studies on the ability of woodchips of different types to remove these common stormwater contaminants and emerging contaminants of concern. Overall, the literature demonstrated woodchips are an inexpensive and effective material that could be implemented for the removal of contaminants in urban stormwater.

SARS-CoV-2 was discovered among humans in late 2019 and rapidly spread across the world. Although the virus is transmitted by respiratory droplets, most infected persons also excrete viral particles in their feces. This fact prompted a range of studies assessing the usefulness of wastewater surveillance to determine levels of infection and transmission and produce early warnings of outbreaks in local communities, independently of human testing. In this study, we collected samples of wastewater from 13 locations across Oklahoma City, representing different population types, twice per week from November 2020 to end of March 2021. Wastewater samples were collected and analyzed for the presence and concentration of SARS-CoV-2 RNA using RT-qPCR. The concentration of SARS-CoV-2 in the wastewater showed notable peaks, preceding the number of reported COVID-19 cases by an average of one week (ranging between 4 and 10 days). The early warning lead-time for an outbreak or increase in cases was significantly higher in areas with larger Hispanic populations and lower in areas with a higher household income or higher proportion of persons aged 65 years or older. Using this relationship, we predicted the number of cases with an accuracy of 81–92% compared to reported cases. These results confirm the validity and timeliness of using wastewater surveillance for monitoring local disease transmission and highlight the importance of differences in population structures when interpreting surveillance outputs and planning preventive action., Graphical abstract Unlabelled Image

This review summarizes over 280 studies published in 2019 related to the characterization, control, and management of urban stormwater runoff. A summary of quantity and quality concerns is provided in the first section of the review, serving as the foundation for the following sections which focus on the control and treatment of stormwater runoff. Finally, the impact of stormwater control devices at the watershed scale is discussed. Each section provides a self-contained overview of the 2019 literature, common themes, and future work. Several themes emerged from the 2019 literature including exploration of substrate amendments for improved water quality effluent from stormwater controls, the continued study of the role of vegetation in green infrastructure practices, and a call to action for the development of new models which generate reliable, computationally efficient results under the physical, chemical, biological, and social complexity of stormwater management. PRACTITIONER POINTS: Over 280 studies were published in 2019 related to the characterization, control, and treatment of urban stormwater. Studies on bioretention and general stormwater characteristics represented the two most common subtopics in 2019. Trends in 2019 included novel substrate amendments, studies on the role of vegetation, and advancements in computational models.

A review of literature related to stormwater runoff characterization and its subsequent management and treatment from 2017 was conducted. The 250 articles summarized herein are organized along three central themes: (1) stormwater quality and quantity characteristics, (2) site-scale stormwater management practices, with a focus on low impact development (LID) and green infrastructure (GI), and (3) watershed-scale performance of stormwater controls. Within each section, common research themes and future work are highlighted.

In 2007, ten bioretention cells were constructed in Oklahoma as part of a full-scale technology project to demonstrate stormwater phosphorus reduction. The filter media used was amended with 5%, Class C fly ash by weight to increase phosphorus and heavy metal retention. In 2014, core samples were collected from four of the cells, and three were instrumented for continuous water monitoring for the following year. This paper will review the design, construction, computer modeling of phosphorus retention, and measured phosphorus removal after seven years of operation. Total phosphorus retained in the sampled cells showed reductions in effluent water concentrations of 68 to 75%, while total effluent mass reductions of 51 to 93% were achieved. Total phosphorus accumulation in the cells measured in cores ranged from 0.33 to 0.60 kg/year, which was somewhat greater than the annual calculated effluent reduction of 0.27 to 0.41 kg/year. While good, phosphorus retention was not as high as computer modeling predicted. Other research on the cells, including hydraulics, heavy metal adsorption, and microbial transport, is summarized. Experimental challenges with phosphorus extraction from samples are also discussed. All experience and results suggest that fly ash amendments are an effective option for phosphorus removal in bioretention cells.

A summary of 246 studies published in 2016 on topics related to the characterization and management of urban stormwater runoff is presented in the following review. The review is structured along three major topical areas: (1) general characterization of stormwater quantity and quality; (2) engineered systems for stormwater control and treatment, including erosion and sediment control practices, constructed stormwater ponds and wetlands, bioretention, permeable pavement, greenroofs, and rainwater harvesting and (3) watershedscale application of stormwater treatment and control practices. Common research themes and needs highlighted throughout this review include efforts to better understand stormwater transport and treatment mechanisms and their representation in models, advancements to optimize the design of stormwater control measures to meet specific hydrologic and/or water quality targets, and increasing understanding of the biophysical and social factors that influence watershed-scale implementation of low impact development and other stormwater control measures.

Plant nursery runoff commonly contains pesticides and nutrients that often threaten aquatic ecosystems. Constructed wetlands could be a tool to remove pesticides and nutrients from nursery runoff but have not been extensively studied in this setting. Two field-scale constructed wetlands (one subsurface-flow constructed wetland [SFCW] and one free-surface constructed wetland [FSCW]) were implemented and monitored for water quality improvement. The SFCW demonstrated significant mass reduction of 78% or greater for nitrate, orthophosphate, total nitrogen, total phosphorus, and total suspended solids. The SFCW also demonstrated significant mass reduction of 79% or greater for 10 of the 12 pesticide compounds detected in over half of the collected samples. The FSCW demonstrated significant mass reduction of 46% or greater for all nonpesticide analytes except total nitrogen. Loading rate and actual storage volume compared with inflow volume likely affected performance. Reduced size and increased loading rate of the FSCW likely reduced its ability to effectively reduce pesticides. Results from this study indicate that constructed wetlands are likely an effective tool for nursery runoff management. When designing and implementing constructed wetlands, it is important for practitioners to consider the tradeoff between system size (additional cost and land otherwise dedicated to production) and performance.

Bioretention cells (BRCs) have shown potential for storm water quantity and quality control. However, the phosphorus (P) removal in BRC has been variable due to differences of soil properties in filter media. The objectives of this research were to identify and evaluate P accumulation in filter media and to quantify effluent P reduction in BRC. Each cell has a sand and fly ash media designed to remove phosphorous. Filter media were collected in 2014 across the cell surface and to a depth of 0.6 m to quantify the P accumulation. The mean total P (T-P) concentration increased over the seven years of operation, but the changes were not statistically significant. The average Mehlich-3 P (M3-P) and water-soluble P (WS-P) concentrations in the media profiles showed higher P accumulation in the top 0.15 m. The average M3-P and WS-P concentrations between 0.15 m to 0.30 m, and 0.30 m to 0.60 m were variable on all four BRCs media. The media with 5% fly ash significantly retained M3-P and WS-P over the top 0.15 m. Stormwater influent and effluent samples from three of the BRCs monitored over one year showed reductions in both P concentration (68% to 75%) and P mass (76% to 93%).

Microbial pollution in stormwater is a concern in urban areas across the U.S. and is a leading cause of water-quality impairment in the United States. This issue may be addressed through the use of best management practices (BMPs) and target limits for pathogenic indicator species. Bioretention is a commonly used low impact development strategy that addresses this growing pollution problem at the source. Bioretention removal efficiencies have been well studied when considering nutrients and heavy metals, but field-scale treatment data are limited for microbial indicators. The primary objective of this study was to quantify microbial removal by installed bioretention cells with fly-ash amended soils. Three bioretention cells in Grove, Oklahoma were monitored over one and a half years and the removal microbial efficiency was quantified. Overall, removal rates for E. coli, enterococci, and coliphage were highly variable, with mean and standard deviations for removals for each site respectively: E. coli 87%, 35%, and 43%; enterococci 97%, 95%, and 80%; and coliphage 38%, 75%, and 32%. The site with negative removal efficiency appears to have some groundwater intrusion during storm events. Based on this relatively limited data set, these fly-ash amended bioretention cells performed 49% better than those with a sand-only filter media layer currently reported in the literature. Based on this initial field study, it appears that fly-ash amended bioretention cells may be a viable option for enhanced microbial removal from stormwater runoff.

Tree nurseries and greenhouses within the USDA red imported fire ant (RIFA) quarantine zone are required to incorporate insecticides into their potting media to prevent artificial spread of RIFA. Bifenthrin and fipronil are two common insecticides that are incorporated into potting media. During irrigation and stormwater events, there is potential for insecticides to leach from nursery pots, resulting in the contamination of nearby surface waters. In this study, occurrences of insecticides in simulated nursery runoff were compared with two irrigation strategies and two types of containers in single pot leaching and field runoff simulations. In addition, toxicity of pot leachate to the aquatic invertebrate, Hyallela azteca, was measured, and removal efficiencies of insecticides from bioretention cell media were evaluated. Overhead irrigation resulted in significantly higher concentrations than drip irrigation, and RootMaker® pots allowed more leaching as compared to standard slick-wall pots. However, in all tests, the average concentration of bifenthrin during 15 days of leaching in both pot and field simulations was greater than 200 ng/L; more than 100-fold greater than the LC50 for H. azteca. Toxicity studies confirmed this level of toxicity. Higher amounts of compost, 20 and 40 %, in bioretention cell media resulted in greater percent reduction of both bifenthrin and fipronil. This study determined that management techniques may be able to limit the amount of insecticide that leaches from pots and runs off to receiving water bodies. Specifically, the selection of appropriate pot types, irrigation strategies, or filtering runoff through bioretention cells may reduce contamination loads. Thus, further best management strategies such as the use of bioretention cells are needed in nursery and greenhouse facilities to prevent surface water runoff from transporting toxic insecticides.

The following review presents a synthesis of 181 journal articles published during 2015 that represent progress toward better characterizing, controlling, and treating urban stormwater runoff. The review is structured by general topical areas related to (1) stormwater quality and quantity characterization; (2) engineered stormwater control and treatment practices, including erosion and sediment control, stormwater ponds, constructed stormwater wetlands, bioretention, permeable pavement, greenroofs, and rainwater harvesting systems; and (3) watershed-scale modeling and optimization of stormwater control and treatment practices. Common research themes emerging from this collection of studies include potential to enhance hydrologic and pollutant treatment performance of stormwater practices via media amendments and the use of innovative outlet control structures, as well as development of a more mechanistic understanding of hydrologic and water-quality functions to inform modeling and performance predictions. These studies serve to expand the field's knowledge base and will inform future efforts to further improve stormwater control and treatment at various spatial and temporal scales.

Since its inception, Low Impact Development (LID) has become part of urban stormwater management across the United States, marking progress in the gradual transition from centralized to distributed runoff management infrastructure. The ultimate goal of LID is full, cost-effective implementation to maximize watershed-scale ecosystem services and enhance resilience. To reach that goal in the Great Plains, the multi-disciplinary author team presents this critical review based on thirteen technical questions within the context of regional climate and socioeconomics across increasing complexities in scale and function. Although some progress has been made, much remains to be done including continued basic and applied research, development of local LID design specifications, local demonstrations, and identifying funding mechanisms for these solutions. Within the Great Plains and beyond, by addressing these technical questions within a local context, the goal of widespread acceptance of LID can be achieved, resulting in more effective and resilient stormwater management.

Beef cattle feedlots contain unconsolidated surface materials (loose manure pack) that accumulate during a feeding cycle. The effects of varying amounts of unconsolidated surface materials on runoff nutrient losses are not well understood. The objectives of this study were to (1) compare runoff nutrient losses from feedlot surfaces containing varying amounts of unconsolidated surface materials, (2) determine if differences in runoff nutrient losses exist among rainfall simulation runs, (3) relate runoff nutrient losses to selected feedlot soil characteristics, and (4) identify the effects of varying runoff rate on nutrient loss rates from feedlot surfaces. This study was conducted on 0.75 m wide by 2 m long (2.47 ft wide by 6.58 ft long) plots containing 0, 6.7, 13.5, or 26.9 kg m−2 (0, 1.37, 2.77, or 5.51 lb ft−2) of unconsolidated surface materials. Simulated rainfall was applied during three 30-minute events that were separated by 24-hour intervals. Inflow was added at the top of all plots during selected tests to examine the effects of varying flow rate on nutrient loss rates. No significant differences in the measured water quality parameters were found among the surfaces containing varying amounts of unconsolidated surface materials. Measurements of dissolved phosphorus, particulate phosphorus, total phosphorus, ammonium nitrogen, chloride, total dissolved solids, electrical conductivity, and erosion consistently decreased during the three rainfall simulation runs. Runoff losses of ammonium nitrogen (NH4-N), total nitrogen, and nitrate nitrogen were all correlated to easily obtained soil EC measurements. All measured water quality parameters were significantly influenced by runoff rate. Thus, runoff rate, and not the amount of unconsolidated surface materials on the feedlot surface, significantly influenced nutrient losses in runoff.

Surface water bodies can be impaired by turbidity and excessive sediment loading due to urban development, construction activities, and agricultural practices. Turbidity has been considered as a proxy for evaluating water quality, aquatic habitat, and aesthetic impairments in surface waters. The US Environment Protection Agency (USEPA) has listed turbidity and sediment as major pollutants for construction site effluent. Recently proposed USEPA regulations for construction site runoff led to increased interest in methods to predict turbidity in runoff based on parameters that are more commonly predicted in runoff–erosion models. In this study, a turbidity prediction methodology that can be easily incorporated into existing runoff–erosion models has been developed using fractions of sand, silt, and clay plus suspended sediment concentration of eight parent soils from locations in Oklahoma and South Carolina, USA.

The size distribution of sediment in runoff from feedlot surfaces influences erosion rates and settling velocity. The objectives of this study were to: (1) measure runoff, erosion, and size distribution of sediment in runoff from feedlot surfaces containing varying amounts of unconsolidated surface material (USM), and (2) determine the effects of varying runoff rate on erosion and sediment size distribution. Simulated rainfall was applied to 0.75 m wide by 2 m long plots located within feedlot pens. Sieve and pipette analyses were used to measure the diameters of the eroded materials. No significant differences in runoff and erosion were found among the treatments with varying amounts of USM. Values for D50, the size for which 50% of the sediment is smaller, were 36 m or less for each of the treatments containing varying amounts of USM. The surfaces containing 0 or 6.7 kg m -2 of USM had D50 values that were significantly greater than those with 13.5 or 26.9 kg m -2 of USM. An increase in runoff rate resulted in significantly greater erosion. The proportion of sediment fractions 31 m and larger consistently increased as runoff rate became greater. No significant differences in D50 values were found for runoff rates varying from 0.5 to 9.7 kg min -1 . The D50 value of 310 m obtained at a flow rate of 15.3 kg min -1 was significantly greater than measurements determined at the other runoff rates. Both erosion and size distribution of sediment in runoff from feedlot surfaces are significantly influenced by runoff rate.

The use of pond ash (fly ash that has been placed in evaporative ponds for storage and subsequently dewatered) for feedlot surfaces provides a drier environment for livestock and furnishes economic benefits. However, pond ash is known to have high concentrations of trace elements, and the runoff water-quality effects of feedlot surfaces amended with pond ash are not well defined. For this study, two experimental units (plots) were established in eight feedlot pens. Four of the pens contained unamended soil surfaces, and the remaining four pens had pond-ash amended surfaces. Before each test, unconsolidated surface material was removed from four of the plots for each of the amendment treatments, resulting in eight unamended plots and eight pond-ash amended plots. Concentrations for 23 trace elements were measured in cattle feedlot surface material and in the runoff water from three simulated rainfall events. Trace element concentrations in surface material and runoff did not differ between surface consolidation treatments. Amending the feedlot surface material with pond ash resulted in a significant increase in concentration for 14 of the 17 trace elements. Runoff concentrations for 21 trace elements were affected by pond-ash amendment. Sixteen of 21 trace element concentrations that differed significantly were greater in runoff from unamended soil surfaces. Concentrations in runoff were significantly correlated with concentrations in feedlot surface material for boron, manganese, molybdenum, selenium, and uranium.

Distillers byproducts can serve as valuable sources of protein and energy for beef cattle. However, the water quality effects of the use of distillers byproducts in cattle rations are not well understood. The objectives of this study were to: (1) measure soil properties and nutrient transport in runoff from feedlot surfaces as affected by corn-based and wet distillers grain diets, (2) compare the effects of unconsolidated surface materials and consolidated subsurface materials on feedlot soil characteristics and runoff nutrient transport, (3) determine if runoff nutrient transport from feedlot surfaces is correlated to selected feedlot soil properties, and (4) identify the effects of varying runoff rate on nutrient transport. Simulated rainfall events were applied to 0.75 m wide by 2 m long plots. Concentrations of calcium, copper, loss on ignition, magnesium, organic-N, potassium, total N (TN), and zinc in the feedlot soil materials were significantly greater within the pens where cattle were fed a corn-based diet rather than a diet with distillers grain. The pens where cattle were fed distillers grain contained significantly greater amounts of Bray-1 P. Surface condition did not significantly affect any of the measured feedlot soil properties except potassium content. No significant differences in measured runoff water quality parameters were found between the corn-based and wet distillers grain treatments. Runoff measurements of NH4-N, TN, NO3-N, total dissolved solids, and electrical conductivity were each significantly correlated to seven or more feedlot soil parameters. Each of the measured water quality parameters were significantly influenced by runoff rate.

The addition of pond ash (fly ash that has been placed in evaporative ponds and subsequently dewatered) to feedlot surfaces provides a healthier environment for livestock and economic advantages for the feedlot operator. However, the water quality effects of pond ash amended surfaces are not well understood. The objectives of this field investigation were to: (1)�compare feedlot soil properties, and nutrient and bacterial transport in runoff, from pond ash amended surfaces and soil surfaces; (2) compare the effects of unconsolidated surface materials (USM) (loose manure pack) and consolidated subsurface materials (CSM) (compacted manure and underlying layers) on nutrient and bacterial transport in runoff; and (3) determine if the measured water quality parameters are correlated to soil properties. Simulated rainfall events were applied to 0.75 m wide × 2 m long plots with different surface materials and surface conditions. Measurements of calcium, magnesium, sulfur, and pH were found to be significantly greater on the pond ash amended surfaces. In comparison, the soil surfaces contained significantly greater amounts of Bray 1-P. The runoff load of NH4-N was significantly greater on the pond ash amended surfaces, while the total phosphorus (TP) load was significantly greater on the soil surfaces. The NO3-N and total nitrogen (TN) loads in runoff were significantly greater on the feedlot surfaces containing CSM. Concentrations of E.�coli in runoff were similar on the pond ash amended surfaces and soil surfaces. The dissolved phosphorus (DP), particulate phosphorus (PP), and TP load of runoff were all significantly correlated to Bray 1-P measurements.

Estimates of streambed water flux are needed for the interpretation of streambed chemistry and reactions. Continuous temperature and head monitoring in stream reaches within four agricultural watersheds (Leary Weber Ditch, IN; Maple Creek, NE; DR2 Drain, WA; and Merced River, CA) allowed heat to be used as a tracer to study the temporal and spatial variability of fluxes through the streambed. Synoptic methods (seepage meter and differential discharge measurements) were compared with estimates obtained by using heat as a tracer. Water flux was estimated by modeling one-dimensional vertical flow of water and heat using the model VS2DH. Flux was influenced by physical heterogeneity of the stream channel and temporal variability in stream and ground-water levels. During most of the study period (April-December 2004), flux was upward through the streambeds. At the IN, NE, and CA sites, high-stage events resulted in rapid reversal of flow direction inducing short-term surface-water flow into the streambed. During late summer at the IN site, regional ground-water levels dropped, leading to surface-water loss to ground water that resulted in drying of the ditch. Synoptic measurements of flux generally supported the model flux estimates. Water flow through the streambed was roughly an order of magnitude larger in the humid basins (IN and NE) than in the arid basins (WA and CA). Downward flux, in response to sudden high streamflows, and seasonal variability in flux was most pronounced in the humid basins and in high conductivity zones in the streambed.

Rainfall samples were collected during the 2003 and 2004 growing seasons at four agricultural locales across the USA in Maryland, Indiana, Nebraska, and California. The samples were analyzed for 21 insecticides, 18 herbicides, three fungicides, and 40 pesticide degradates. Data from all sites combined show that 7 of the 10 most frequently detected pesticides were herbicides, with atrazine (70%) and metolachlor (83%) detected at every site. Dacthal, acetochlor, simazine, alachlor, and pendimethalin were detected in more than 50% of the samples. Chlorpyrifos, carbaryl, and diazinon were the only insecticides among the 10 most frequently detected compounds. Of the remaining pesticide parent compounds, 18 were detected in fewer than 30% of the samples, and 13 were not detected. The most frequently detected degradates were deethylatrazine; the oxygen analogs (OAs) of the organophosphorus insecticides chlorpyrifos, diazinon, and malathion; and 1-napthol (degradate of carbaryl). Deethylatrazine was detected in nearly 70% of the samples collected in Maryland, Indiana, and Nebraska but was detected only once in California. The OAs of chlorpyrifos and diazinon were detected primarily in California. Degradates of the acetanilide herbicides were rarely detected in rain, indicating that they are not formed in the atmosphere or readily volatilized from soils. Herbicides accounted for 91 to 98% of the total pesticide mass deposited by rain except in California, where insecticides accounted for 61% in 2004. The mass of pesticides deposited by rainfall was estimated to be less than 2% of the total applied in these agricultural areas.

We evaluated the efficacy, sensitivity, host-specificity, and spatial/temporal dynamics of human- and ruminant-specific 16S rRNA gene Bacteroidetes markers used to assess the sources of fecal pollution in a fecally impacted watershed. Phylogenetic analyses of 1271 fecal and environmental 16S rRNA gene clones were also performed to study the diversity of Bacteroidetes in this watershed. The host-specific assays indicated that ruminant feces were present in 28–54% of the water samples and in all sampling seasons, with increasing frequency in downstream sites. The human-targeted assays indicated that only 3–5% of the water samples were positive for human fecal signals, although a higher percentage of human-associated signals (19–24%) were detected in sediment samples. Phylogenetic analysis indicated that 57% of all water clones clustered with yet-to-be-cultured Bacteroidetes species associated with sequences obtained from ruminant feces, further supporting the prevalence of ruminant contamination in this watershed. However, since several clusters contained sequences from multiple sources, future studies need to consider the potential cosmopolitan nature of these bacterial populations when assessing fecal pollution sources using Bacteroidetes markers. Moreover, additional data is needed in order to understand the distribution of Bacteroidetes host-specific markers and their relationship to water quality regulatory standards.

The occurrence of human enteric viruses in ground water has been well documented in the literature. Bacteriophages such as MS-2 and PRD1 have properties similar to pathogenic human viruses suggesting that bacteriophages can be used as proxies for virus transport. The objective of this study is to investigate a “worst case scenario” for virus transport in a ground water aquifer, i.e., sand and gravel aquifer under a forced-gradient, by using bacteriophages. Field studies have been conducted to trace large-scale (34 m) and small-scale (10 m) virus transport under natural- and forced-gradients through a sand and gravel aquifer at a ground water research site at the Texas A&M University. Virus transport was monitored under natural and forced-gradient conditions using MS-2 and PRD-1 as virus tracers and bromide as a conservative tracer. Results indicate virus and bromide transport to down-gradient monitoring wells in both the large- and small-scale field tests. During the tests conducted, MS-2 transport appears to exhibit little longitudinal dispersion, showing a narrow peak at the well nest 34 m down-gradient in 13 days which is 20 days before the bromide breakthroughs, indicating that bacteriophage transport through the aquifer was mainly by advective flow. Differences in tracer transport can be attributed to the heterogeneity of the sand and gravel aquifer at the site, different injection methods, different sampling methods, and different tracer properties. Heterogeneity of the aquifer would cause virus transport through preferential flow paths.

Research on the fate and transport of endocrine-disrupting compounds and other organic wastewater compounds released into the environment and their potential presence in drinking water is in its infancy. Studies conducted during the last decade in Lincoln, Nebraska, and Berlin, Germany, indicate that removal of less polar compounds probably can be obtained through bank filtration, ground-water enrichment, and additional drinking-water and wastewater treatment processes. Polar compounds, such as atrazine and some metabolites, occur in drinking water obtained from contaminated surface water or ground water, but at concentrations generally lower than those occurring in wastewater and surface water. The results of the studies also suggest that concentrations of nonpolar estrogenic compounds decrease during drinking-water pretreatment processes such as bank filtration and ground-water enrichment.

The utilization of Chloride Mass Balance (CMB) to determine water fluxes generally has been restricted to applications in arid to semi-arid environments. Only in such environments does the chloride deposited by precipitation and dry fallout concentrate sufficiently by evapotranspiration for accurate measurement. This study successfully applied CMB to dryland winter wheat plots with 860 mm of precipitation per year. Soil cores were collected from long-term dryland winter wheat test plots located near Stillwater, Oklahoma, which had known constant applications of the fertilizer KCl for the past 29 years. This additional chloride was sufficient to allow for accurate chloride concentration measurement. Groundwater recharge rates of 12.2 to 38.9 mm/y were calculated with recharge increasing with fertilizer N. These fluxes may be overestimated by up to 20% based on anion exclusion measurements from adjacent soil cores. Numerical modeling of the chloride distributions beneath the plots supported the assumptions of CMB.

The risk to human health of the annual sandhill crane ( Grus canadensis ) migration through Nebraska, which is thought to be a major source of fecal pollution of the central Platte River, is unknown. To better understand potential risks, the presence of Campylobacter species and three fecal indicator bacterial groups ( Enterococcus spp., Escherichia coli , and Bacteroidetes ) was assayed by PCR from crane excreta and water samples collected during their stopover at the Platte River, Nebraska, in 2010. Genus-specific PCR assays and sequence analyses identified Campylobacter jejuni as the predominant Campylobacter species in sandhill crane excreta. Campylobacter spp. were detected in 48% of crane excreta, 24% of water samples, and 11% of sediment samples. The estimated densities of Enterococcus spp. were highest in excreta samples (mean, 4.6 � 10 8 cell equivalents [CE]/g), while water samples contained higher levels of Bacteroidetes (mean, 5.1 � 10 5 CE/100 ml). Enterococcus spp., E. coli , and Campylobacter spp. were significantly increased in river water and sediments during the crane migration period, with Enterococcus sp. densities (∼3.3 � 10 5 CE/g) 2 to 4 orders of magnitude higher than those of Bacteroidetes (4.9 � 10 3 CE/g), E. coli (2.2 � 10 3 CE/g), and Campylobacter spp. (37 CE/g). Sequencing data for the 16S rRNA gene and Campylobacter species-specific PCR assays indicated that C. jejuni was the major Campylobacter species present in water, sediments, and crane excreta. Overall, migration appeared to result in a significant, but temporary, change in water quality in spring, when there may be a C. jejuni health hazard associated with water and crops visited by the migrating birds.

Wild birds have been shown to be significant sources of numerous types of pathogens that are relevant to humans and agriculture. The presence of large numbers of migratory birds in such a sensitive and important ecosystem as the Platte River in central Nebraska, USA, could potentially serve a significant source of bird-derived pathogens in the water/sediment and riverine environment. In 2009 and 2010, a study was completed to investigate the potential water-quality impacts of Sandhill Cranes and Snow Geese on the microbial water quality of the central Platte River during their spring migration period. Fecal material, river-bottom sediment, and water samples were collected from January through May of each year during the spring migration season of Sandhill Cranes in the Central Flyway of North America. Results indicate that several types of fecal indicator bacteria and from a range of viral, protozoan, and bacterial pathogens, Campylobacter jejuni were present in Sandhill Crane excreta, and at significantly higher frequency and densities in water and sediments when the Sandhill Cranes were present, particularly during evening roosts within the Platte River environment. Therefore, further investigation of the health significance of avian pathogens is warranted for the Platte River in Central Nebraska during migration of Sandhill Cranes and other waterfowl.

While the microbial water quality in the Platte River is seasonally impacted by excreta from migrating cranes, there are no methods available to study crane fecal contamination. Here we characterized microbial populations in crane feces using phylogenetic analysis of 16S rRNA gene fecal clone libraries. Using these sequences, a novel crane quantitative PCR (Crane1) assay was developed, and its applicability as a microbial source tracking (MST) assay was evaluated by determining its host specificity and detection ability in environmental waters. Bacteria from crane excreta were dominated by bacilli and proteobacteria, with a notable paucity of sequences homologous to Bacteroidetes and Clostridia . The Crane1 marker targeted a dominant clade of unclassified Lactobacillales sequences closely related to Catellicoccus marimammalium . The host distribution of the Crane1 marker was relatively high, being positive for 69% (66/96) of the crane excreta samples tested. The assay also showed high host specificity, with 95% of the nontarget fecal samples (i.e., n = 553; 20 different free-range hosts) being negative. Of the presumed crane-impacted water samples ( n = 16), 88% were positive for the Crane1 assay, whereas none of the water samples not impacted by cranes were positive ( n = 165). Bayesian statistical models of the Crane1 MST marker demonstrated high confidence in detecting true-positive signals and a low probability of false-negative signals from environmental water samples. Altogether, these data suggest that the newly developed marker could be used in environmental monitoring studies to study crane fecal pollution dynamics.

Sediment is major cause of surface water contamination in the United States and can lead to habitat degradation and water quality impairment. Additionally, sediment transports nutrients, pesticides, and other chemicals that can also be detrimental to receiving ecosystems. A high risk contributor to sediment pollution is disturbed land areas, such as construction sites. Traditionally, detention ponds that rely on gravity settling have been used to capture sediment in runoff from construction sites. Since suspended sediment in runoff is comprised of various size particles with varying settling velocities, a significant fraction is often too fine to settle out in a detention basin of practical size, which leads to sediment transport off the construction site. In order to mitigate the potential for environmental contamination, the EPA is expected to set a maximum turbidity limit for construction site runoff that has yet to be determined.

Sediment in stormwater from construction site runoff consists of particles having a variety of sizes. Fine particles such as fine silt, clay, and colloidal particles remain in suspension and in many cases cannot be effectively removed by conventional sediment control practices. The US EPA is targeting fine silt, clay, and colloids in stormwater as primary pollutants. Some methods that can be employed for removal of the fine sediments from the runoff are gravitational settling, flocculation, filtration and/or a combination of these processes. The objective of the project reported herein was to estimate the coagulation factor and the breakage coefficient for three different soil-flocculant combinations, which are required to model the influence of flocculation on removal of sediment from stormwater runoff. An apparatus has been developed and tested to measure the variables required to estimate these parameters. A mathematical model based on the research work done by Argaman and Kaufman (1970) was used to estimate the flocculation parameters. The measured inputs to the model for estimating the parameters are: particle size class distribution, turbulent diffusion coefficient, sediment input mass flow rate (in l/min), flow rate of water (in l/min), suspended sediment concentration at various sampling stations (in g/L), mass of suspended sediment in reactors at the bottom of the sedimentation basin (in g), and mean square turbulent velocity (in cm/s). The experimental setup consists of a constant head tank, a rectangular flume with sampling ports, flocculant injection, soil separating flume, slurry tank, and oscillating grids. A constant head tank feeds water to a flume at a constant flow rate of 170 l/min. The rectangular flume is 9.1 m long, 0.15 m wide and 0.46 m deep. The flow depth on the flume is set at 0.3 m. Sediment was injected in into the flume in the form of a slurry with sand-sized particles removed. The sediment slurry was stored in a tank and kept well mixed by pass-through circulation and a rotating mixer. Flocculant was injected using a peristaltic pump; the concentration of the flocculant in the flume was maintained at 0.05 g/L. This concentration was determined using jar tests to optimize flocculation. An oscillating grid assembly was used to induce turbulence with a known velocity gradient. Ten sampling ports were used to sample the suspended sediments in the flow. A known concentration of sediment flow was injected into the flume and mass balance equations were used to determine the rate of flocculation. The particle size class distribution of the input slurry was predicted separately using pipette analysis. An acoustic Doppler velocitimeter (ADV) was used to measure the turbulent velocities. The coagulation factor and the breakup coefficient, estimated from the mathematical model are expected to be constant. These can be used by storm water practitioners to model the sediment removal characteristics depending upon various types of soil and flocculants.

Although numerous studies of hyporheic exchange and denitrification have been conducted in pristine, high-gradient streams, few studies of this type have been conducted in nutrient-rich, low-gradient streams. This is a particularly important subject given the interest in nitrogen (N) inputs to the Gulf of Mexico and other eutrophic aquatic systems. A combination of hydrologic, mineralogical, chemical, dissolved gas, and isotopic data were used to determine the processes controlling transport and fate of NO(3)(-) in streambeds at five sites across the USA. Water samples were collected from streambeds at depths ranging from 0.3 to 3 m at three to five points across the stream and in two to five separate transects. Residence times of water ranging from 0.28 to 34.7 d m(-1) in the streambeds of N-rich watersheds played an important role in allowing denitrification to decrease NO(3)(-) concentrations. Where potential electron donors were limited and residence times were short, denitrification was limited. Consequently, in spite of reducing conditions at some sites, NO(3)(-) was transported into the stream. At two of the five study sites, NO(3)(-) in surface water infiltrated the streambeds and concentrations decreased, supporting current models that NO(3)(-) would be retained in N-rich streams. At the other three study sites, hydrogeologic controls limited or prevented infiltration of surface water into the streambed, and ground-water discharge contributed to NO(3)(-) loads. Our results also show that in these low hydrologic-gradient systems, storm and other high-flow events can be important factors for increasing surface-water movement into streambeds.

Pesticide transport through the unsaturated zone is a function of chemical and soil characteristics, application, and water recharge rate. The fate and transport of 82 pesticides and degradates were investigated at five different agricultural sites. Atrazine and metolachlor, as well as several of the degradates of atrazine, metolachlor, acetochlor, and alachlor, were frequently detected in soil water during the 2004 growing season, and degradates were generally more abundant than parent compounds. Metolachlor and atrazine were applied at a Nebraska site the same year as sampling, and focused recharge coupled with the short time since application resulted in their movement in the unsaturated zone 9 m below the surface. At other sites where the herbicides were applied 1 to 2 yr before sampling, only degradates were found in soil water. Transformations of herbicides were evident with depth and during the 4-mo sampling time and reflected the faster degradation of metolachlor oxanilic acid and persistence of metolachor ethanesulfonic acid. The fraction of metolachlor ethanesulfonic acid relative to metolachlor and metolachlor oxanilic acid increased from 0.3 to >0.9 at a site in Maryland where the unsaturated zone was 5 m deep and from 0.3 to 0.5 at the shallowest depth. The flux of pesticide degradates from the deepest sites to the shallow ground water was greatest (3.0-4.9 micromol m(-2) yr(-1)) where upland recharge or focused flow moved the most water through the unsaturated zone. Flux estimates based on estimated recharge rates and measured concentrations were in agreement with fluxes estimated using an unsaturated-zone computer model (LEACHM).

Given known limitations of current microbial source-tracking (MST) tools, emphasis on small, simple study areas may enhance interpretations of fecal contamination sources in streams. In this study, three MST tools-Escherichia coli repetitive element polymerase chain reaction (rep-PCR), coliphage typing, and Bacteroidales 16S rDNA host-associated markers-were evaluated in a selected reach of Plum Creek in south-central Nebraska. Water-quality samples were collected from six sites. One reach was selected for MST evaluation based on observed patterns of E. coli contamination. Despite high E. coli concentrations, coliphages were detected only once among water samples, precluding their use as a MST tool in this setting. Rep-PCR classification of E. coli isolates from both water and sediment samples supported the hypothesis that cattle and wildlife were dominant sources of fecal contamination, with minor contributions by horses and humans. Conversely, neither ruminant nor human sources were detected by Bacteroidales markers in most water samples. In bed sediment, ruminant- and human-associated Bacteroidales markers were detected throughout the interval from 0 to 0.3 m, with detections independent of E. coli concentrations in the sediment. Although results by E. coli-based and Bacteroidales-based MST methods led to similar interpretations, detection of Bacteroidales markers in sediment more commonly than in water indicates that different tools to track fecal contamination (in this case, tools based on Bacteroidales DNA and E. coli isolates) may have varying relevance to the more specific goal of tracking the sources of E. coli in watersheds. This is the first report of simultaneous, toolbox approach application of a library-based and marker-based MST analyses to flowing surface water.