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Purpose: “The purpose of this research study was to investigate the effect of Puritan’s Pride probiotic on gastrointestinal quality of life of working adults aged 18 to 65years at the Loma Linda Support Services Building.” Methods: Working adults, 18 to 65years of age, from the Loma Linda Support Services Building were eligible to participate in the study. Subjects were excluded if they were taking antibiotics or had taken antibiotics within the previous six months. Subjects who consented were given an initial Gastrointestinal Quality of Life Index (GIQLI)1 test and a probiotic supplement for 60days. Subjects’ body composition was also tested during the initial visit. After the 60 days of supplementation, subjects returned to retake the GIQLI and to retest their body composition. Results: Eleven subjects, 1 male and 10 female, reported for post intervention GIQLI and body composition testing. A mean increase of 10.7 (SD= 11.1) in GIQLI scores was found after probiotic supplementation (p= .009). No significant differences were found in pre and post intervention measurements for BMI, body fat, and weight. The best predictor of post intervention GIQLI scores was pre intervention GIQLI scores. Conclusion: There was an improvement in the related gastrointestinal quality of life in our subjects as shown through a significant increase in GIQLI scores and subjects’ commentary. The best predictor of posttest GIQLI scores was pretest GIQLI scores.