Search

The rationale for applying agricultural measures and practices is their confirmed, favourable effect on the growth, development and yield of plants. Each of them, however, interacts with the environmental conditions, particularly soil quality and precipitation distribution during the growing season. The aim of this study was to find the effect of bioregulators applied at different nitrogen fertilization levels and in years with different amounts and distributions of rainfall on some biometric features, yield components and spring wheat yield. A two-factor field experiment was carried out for 4 years differing in the amount and distribution of rainfall during the growing period of spring wheat (2010-2013). Experimental factors were: A – nitrogen fertilization: N1 – 60 kg N·ha-1, N2 – 120 kg N·ha-1; B – bioregulators: B1-nitrophenols (NPH), B2 – trinexapac-ethyl (TE), B3 – chlorocholine chloride (CCC), B4 – TE + NPH, B5 – CCC + NPH, B6 – no bioregulators (control). The experiment was established in the randomized split-plot design in four replications. In three of the four years of the study there occurred a significant independent effect of nitrogen fertilization and bioregulators application, as well as a combined effect of those factors on spring wheat grain and straw yields. The effect of bioregulators on the number of grains per spike and on the yields was dependent on the year of the study. In the year with a large rainfall deficit from intensive stem elongation, TE application increased the grain yield, whereas CCC application reduced it, but not significantly. These effects were abolished by the combined use of NPH with growth retardants. Nevertheless, in these site conditions the unfavourable effect of CCC on grain yield was increased by additional fertilization with nitrogen. TE and CCC also decreased the yield when rainfall deficit occurred in May, at tillering. [ABSTRACT FROM AUTHOR]

To test the hypothesis that the omental lymphoid organ (OLO) made by peritoneal milky spots is either a source of haemopoietic (microphage) progenitors or growth factors we attempted to culture OLO cells in vitro in a variety of assay combinations. With the culture in vitro in semisolid agar it was found that OLO cells do not form granulocyte macrophage or macrophage colonies in response to stimulants. However, when in the same assay marrow cells were used as the targets and OLO-related preparations as stimulants it was observed that marrow cells formed exclusively macrophage colonies. These marrow cells, in response to stimulants derived from other organs, produced granulocyte macrophage, granulocyte and macrophage colonies, OLO-related preparations tested for macrophage-colony stimulating activity included partly purified medium conditioned by OLO cells derived from mice, either injected with endotoxin or not, and medium conditioned by OLO cells after 14 days, liquid culture in vitro. While these results were observed in Swiss mice. C3H/W mice, which arc genetically endotoxinunresponsive, failed to show this reaction. These data may suggest that the local production of macrophage-colony stimulating activity in the peritoneal cavity could be one physiological role for OLO. OLO is the first organ in adult mice identified to stimulate exclusively macrophage colony growth, and not granulocyte-macrophage or pure granulocyte colonies. [ABSTRACT FROM AUTHOR]

Growing awareness of the risks posed by pollution of the soil environment is leading to the development of new remediation strategies. The technique of aided phytostabilization, which involves the evaluation of new heavy-metal (HM)-immobilizing amendments, together with appropriately selected plant species, is a challenge for environmental protection and remediation of the soil environment, and seems to be promising. In this study, the suitability of bentonite for the technique of aided phytostabilization of soils contaminated with high HM concentrations was determined, using a mixture of two grass species. The HM contents in the tested plants and in the soil were determined by flame atomic absorption spectrometry. The application of bentonite had a positive effect on the biomass of the tested plants, and resulted in an increase in soil pH. The concentrations of copper, nickel, cadmium, lead and chromium were higher in the roots than in the above-ground parts of the plants, especially when bentonite was applied to the soil. The addition of the analyzed soil additive contributed significantly to a decrease in the levels of zinc, copper, cadmium and nickel in the soil at the end of the experiment. In view of the above, it can be concluded that the use of bentonite in the aided phytostabilization of soils polluted with HMs, is appropriate.

Nanotechnology and nanomaterials, including silver nanoparticles (AgNPs), are increasingly important in modern science, economics, and agriculture. Their biological activity involves influencing plant health, physiological processes, growth, and yields, although they can also be toxic in the environment. A new fertiliser was made based on a urea solution with a relatively low content of AgNPs obtained by the reduction of silver nitrate V. Laboratory tests were used to assess the effect of a fertiliser solution containing 10 ppm AgNPs on the germination of agricultural plant seeds (barley, peas, oilseed rape) and vegetables (radish, cucumber, lettuce) and its foliar application on chlorophyll content, stomatal conductance, and seedling biomass. Field experiments were conducted to assess the effect that a foliar application of 15 ppm AgNPs in working liquid had on physiological plant parameters and yields of rape and cucumber. The AgNPs in the tested fertiliser reduced infestation of the germinating seeds by pathogens and positively affected the physiological processes, productivity, and yields of plants. Plant response depended on plant species and habitat conditions. Reduced pathogen infestation of seeds, higher germination energy, increased chlorophyll content and stomatal conductance, and higher seedling masses all occurred under the influence of AgNPs, mainly in oilseed rape and cucumber, and especially under thermal stress. The beneficial effect of AgNPs on the yield of these plants occurred in years of unfavourable weather conditions. The positive agricultural test results, especially under stress conditions, indicate that fertiliser produced with AgNPs as an ingredient may reduce the use of pesticides and highly concentrated mineral fertilisers. Such a fertiliser is fully in line with the idea of sustainable agriculture. However, research on the effects that AgNPs and fertiliser have on the environment and humans should continue.

Efficient use of scarce water resources is both a marketing objective and an environmental obligation for sustainable agriculture. In modern agricultural production, which is intensive and should at the same time be environmentally friendly, there is a need to monitor soil moisture, salinity and temperature. The aim of the study was to determine the demand of producers of agricultural and horticultural plants for equipment and systems for monitoring soil properties at an individual farm level in regions with highly developed agriculture. A questionnaire survey was conducted among 1087 respondents, also direct interviews in Poland were undertaken. According to the producers' responses, it is important to know soil moisture, salinity and temperature, although currently only about 4% of the surveyed farmers have the equipment to evaluate these soil parameters. In their view cost is not the most important obstacle to the purchase of the necessary probes. More important is that the devices should be easy to install and use, and have an easy to use application for data collection, processing and transfer. The current market does not offer solutions that meet these producers expectations. The demand for suitable probes is very high as over 80% of the farmers declared their willingness to purchase such probes. Technical problems related to the operation and servicing of such equipment were the most frequently mentioned impediments in their use. However, farmers and horticulturists believe that knowledge of their soil properties would allow them to optimize the elements of cultivation technology, including the use of plant irrigation systems, the use of mineral fertilizers and plant protection products., (© 2021. The Author(s).)

In recent years, a lot of attention has been given to searching for new additives which will effectively facilitate the process of immobilizing contaminants in the soil. This work considers the role of the enhanced nano zero valent iron (nZVI) strategy in the phytostabilization of soil contaminated with potentially toxic elements (PTEs). The experiment was carried out on soil that was highly contaminated with PTEs derived from areas in which metal waste had been stored for many years. The plants used comprised a mixture of grasses- Lolium perenne L. and Festuca rubra L. To determine the effect of the nZVI on the content of PTEs in soil and plants, the samples were analyzed using flame atomic absorption spectrometry (FAAS). The addition of nZVI significantly increased average plant biomass (38%), the contents of Cu (above 2-fold), Ni (44%), Cd (29%), Pb (68%), Zn (44%), and Cr (above 2-fold) in the roots as well as the soil pH. The addition of nZVI, on the other hand, was most effective in reducing the Zn content of soil when compared to the control series. Based on the investigations conducted, the application of nZVI to soil highly contaminated with PTEs is potentially beneficial for the restoration of polluted lands.

A 6-yr-old girl with congenital corticosteroid-resistant pure red cell aplasia was treated with bone marrow transplant from her HLA-identical, MLC-unreactive sister in November 1984 following conditioning with busulfan and cyclophosphamide. Full engraftment was obtained and the patient at 21 months post-transplant is in excellent clinical condition maintaining normal red cell counts. We conclude that BMT should be considered as a therapy for at least the most severe cases of Diamond-Blackfan anaemia resistant to corticosteroids. Successful outcome of this therapy provides an argument for the stem cell origin of this disorder.

Microinjection of recombinant plasmids containing bovine papillomavirus type 1 DNA into the nuclei of mouse C127 cells results in the stimulation of cellular DNA synthesis. Mutations in the viral E2 gene have no apparent effect on this activity even though the same mutations prevent efficient C127 cell focus formation and inhibit transactivation by this gene.

We have studied the transcription rates and the steady-state levels of cytoplasmic mRNA of selected genes in tsAF8 cells, a temperature-sensitive mutant of RNA polymerase II. At the restrictive temperature, the transcription rates decrease uniformly for all genes examined, while the corresponding decreases in steady-state levels of cytoplasmic mRNA vary for individual genes. tsAF8 cells can be advantageously used to study the half-line of mRNA without the use of perturbing drugs.

The proliferating cell nuclear antigen (PCNA or cyclin) is a nuclear protein recently identified as a cofactor of DNA polymerase delta. When exponentially growing Balb/c3T3 cells are exposed to antisense oligodeoxynucleotides to PCNA, both DNA synthesis and mitosis are completely suppressed. A corresponding sense oligodeoxynucleotide has no inhibitory effects. These experiments indicate that PCNA (cyclin) is important in cellular DNA synthesis and in cell cycle progression.

A 72,000 mol wt protein designated PABP binds to the poly(A)+ track of messenger RNAs with high affinity and has been suggested to play an important role in mRNA metabolism in eucaryotic cells. We have employed a human PABP cDNA probe to study the expression of this gene at the mRNA level in BALB/c3T3 mouse cells under different growth conditions and in exponentially growing HeLa cells throughout the cell division cycle. We describe experiments which establish that in BALB/c3T3 cells the expression of this gene is growth factor regulated. Moreover, the gene behaves like a primary response gene in that its induction in quiescent cells does not require the prior synthesis of other growth factor-regulated proteins. In exponentially growing HeLa cells PABP mRNA is expressed throughout the cell division cycle indicating that the expression of this gene is not limited to a specific phase of the cell cycle.