33 results on '"Jasielczuk I"'
Search Results
2. The use of runs of homozygosity for estimation of recent inbreeding in Holstein cattle
- Author
-
Gurgul, A., Szmatoła, T., Topolski, P., Jasielczuk, I., Żukowski, K., and Bugno-Poniewierska, M.
- Published
- 2016
- Full Text
- View/download PDF
3. Genome-wide characteristics of copy number variation in Polish Holstein and Polish Red cattle using SNP genotyping assay
- Author
-
Gurgul, A., Jasielczuk, I., Szmatoła, T., Pawlina, K., Ząbek, T., Żukowski, K., and Bugno-Poniewierska, M.
- Published
- 2015
- Full Text
- View/download PDF
4. Linkage disequilibrium, haplotype blocks and historical effective population size in Arabian horses and selected Polish native horse breeds
- Author
-
Jasielczuk, I., primary, Gurgul, A., additional, Szmatoła, T., additional, Semik-Gurgul, E., additional, Pawlina-Tyszko, K., additional, Stefaniuk-Szmukier, M., additional, Polak, G., additional, Tomczyk-Wrona, I., additional, and Bugno-Poniewierska, M., additional
- Published
- 2020
- Full Text
- View/download PDF
5. Linkage disequilibrium and haplotype block structure in Limousin, Simmental and native Polish Red cattle
- Author
-
Jasielczuk, I., primary, Gurgul, A., additional, Szmatoła, T., additional, Ząbek, T., additional, Pawlina, K., additional, Semik, E., additional, and Bugno-Poniewierska, M., additional
- Published
- 2016
- Full Text
- View/download PDF
6. Identification of genome-wide selection signatures in the Limousin beef cattle breed
- Author
-
Gurgul, A., primary, Szmatoła, T., additional, Ropka-Molik, K., additional, Jasielczuk, I., additional, Pawlina, K., additional, Semik, E., additional, and Bugno-Poniewierska, M., additional
- Published
- 2015
- Full Text
- View/download PDF
7. Identification of genome-wide selection signatures in the Limousin beef cattle breed.
- Author
-
Gurgul, A., Szmatoła, T., Ropka‐Molik, K., Jasielczuk, I., Pawlina, K., Semik, E., and Bugno‐Poniewierska, M.
- Subjects
BEEF cattle breeds ,HOMOZYGOSITY ,HAPLOTYPES ,GENETIC recombination ,CATTLE growth - Abstract
The study is aimed at identifying selection footprints within the genome of Limousin cattle. With the use of Extended Haplotype Homozygosity test, supplemented with correction for variation in recombination rates across the genome, we created map of selection footprints and detected 173 significant (p < 0.01) core haplotypes being potentially under positive selection. Within these regions, a number of candidate genes associated inter alia with skeletal muscle growth ( GDF15, BMP7, BMP4 and TGFB3) or postmortem proteolysis and meat maturation ( CAPN1 and CAPN5) were annotated. Noticeable clusters of selection footprints were detected on chromosomes 1, 4, 8 and 14, which are known to carry several quantitative trait loci for growth traits and meat quality. The study provides information about the genes and metabolic pathways potentially modified under the influence of directional selection, aimed at improving beef production characteristics in Limousin cattle. [ABSTRACT FROM AUTHOR]
- Published
- 2016
- Full Text
- View/download PDF
8. Pet ownership in pregnancy and methylation pattern in newborns
- Author
-
Hanna Danielewicz, Gurgul, A., Anna Dębińska, Grzegorz Myszczyszyn, Szamtola, T., Myszkal, A., Jasielczuk, I., Anna Drabik-Chamerska, Lidia Hirnle, and Andrzej Boznański
9. The use of SNP markers for cattle breed identification.
- Author
-
Jasielczuk I, Gurgul A, Szmatoła T, Radko A, Majewska A, Sosin E, Litwińczuk Z, Rubiś D, and Ząbek T
- Subjects
- Animals, Cattle genetics, Genetic Markers, Poland, Oligonucleotide Array Sequence Analysis, Genetics, Population, Algorithms, Polymorphism, Single Nucleotide genetics, Breeding, Genotype
- Abstract
A potential application of single nucleotide polymorphisms (SNPs) in animal husbandry and production is identification of the animal breed. In this study, using chosen marker selection methods and genotypic data obtained with the use of Illumina Bovine SNP50 BeadChip for individuals belonging to ten cattle breeds, the reduced panels containing the most informative SNP markers were developed. The suitability of selected SNP panels for the effective and reliable assignment of the studied individuals to the breed of origin was checked by three allocation algorithms implemented in GeneClass 2. The studied breeds set included both Polish-native breeds under the genetic resources conservation programs and highly productive breeds with a global range. For all of the tested marker selection methods ("delta" and two F
ST -based variants), two separate methodological approaches of marker assortment were used and three marker panels were created with 96, 192, and 288 SNPs respectively, to determine the minimum number of markers required for effective differentiation of the studied breeds. Moreover, the usefulness of the most effective panels of markers to assess the population structure and genetic diversity of the analyzed breeds was examined. The conducted analyses showed the possibility of using SNP subsets from medium-density genotypic microarrays to distinguish breeds of cattle kept in Poland and to analyze their genetic structure., (© 2024. The Author(s), under exclusive licence to Institute of Plant Genetics Polish Academy of Sciences.)- Published
- 2024
- Full Text
- View/download PDF
10. Transcript level of telomerase reverse-transcriptase (TERT) gene in the rainbow trout (Oncorhynchus mykiss) eggs with different developmental competence for gynogenesis.
- Author
-
Ocalewicz K, Kuciński M, Jasielczuk I, Gurgul A, Kucharski M, and Dobosz S
- Abstract
Expression of the telomerase reverse-transcriptase (TERT) gene and activity of telomerase have been reported in the somatic tissues and gonads in fish irrespective of their age and size. Nevertheless, little is known about TERT expression in the fish eggs. In the current study, the presence of the TERT transcripts was confirmed in the rainbow trout ovulated eggs before and after activation with nonirradiated and UV-irradiated (gynogenesis) sperm. Eggs originating from eight females had high and comparable quality expressed by similar hatching rates. However, survival of the gynogenetic larvae that hatched from eggs activated with UV-irradiated sperm and further exposed to the high hydrostatic pressure (HHP) shock for duplication of the maternal chromosomes varied between females from 2.1 ± 0.4 to 40.5 ± 2.2%. Increased level of TERT transcripts was observed in eggs originating from two females, and gametes from only one of them showed improved competence for gynogenesis (27.3 ± 1.9%). In turn, eggs from the female that exhibited the highest survival after gynogenetic activation were characterized by the lowest expression of the TERT gene. Telomerase in rainbow trout eggs may compensate erosion of the telomeres during early embryonic development; however, its upregulation does not assure better development after gynogenetic activation., (© 2024. The Author(s).)
- Published
- 2024
- Full Text
- View/download PDF
11. Application of Nanopore Sequencing for High Throughput Genotyping in Horses.
- Author
-
Gurgul A, Jasielczuk I, Szmatoła T, Sawicki S, Semik-Gurgul E, Długosz B, and Bugno-Poniewierska M
- Abstract
Nanopore sequencing is a third-generation biopolymer sequencing technique that relies on monitoring the changes in an electrical current that occur as nucleic acids are passed through a protein nanopore. Increasing quality of reads generated by nanopore sequencing systems encourages their application in genome-wide polymorphism detection and genotyping. In this study, we employed nanopore sequencing to identify genome-wide polymorphisms in the horse genome. To reduce the size and complexity of genome fragments for sequencing in a simple and cost-efficient manner, we amplified random DNA fragments using a modified DOP-PCR and sequenced the resulting products using the MinION system. After initial filtering, this generated 28,426 polymorphisms, which were validated at a 3% error rate. Upon further filtering for polymorphism and reproducibility, we identified 9495 SNPs that reflected the horse population structure. To conclude, the use of nanopore sequencing, in conjunction with a genome enrichment step, is a promising tool that can be practical in a variety of applications, including genotyping, population genomics, association studies, linkage mapping, and potentially genomic selection.
- Published
- 2023
- Full Text
- View/download PDF
12. Assessment and Distribution of Runs of Homozygosity in Horse Breeds Representing Different Utility Types.
- Author
-
Szmatoła T, Gurgul A, Jasielczuk I, Oclon E, Ropka-Molik K, Stefaniuk-Szmukier M, Polak G, Tomczyk-Wrona I, and Bugno-Poniewierska M
- Abstract
The present study reports runs of homozygosity (ROH) distribution in the genomes of six horse breeds (571 horses in total) representing three horse types (primitive, light, and draft horses) based on the 65k Equine BeadChip assay. Of major interest was the length, quantity, and frequency of ROH characteristics, as well as differences between horse breeds and types. Noticeable differences in the number, length and distribution of ROH between breeds were observed, as well as in genomic inbreeding coefficients. We also identified regions of the genome characterized by high ROH coverage, known as ROH islands, which may be signals of recent selection events. Eight to fourteen ROH islands were identified per breed, which spanned multiple genes. Many were involved in important horse breed characteristics, including WFIKNN2 , CACNA1G , STXBP4 , NOG , FAM184B , QDPR , LCORL , and the zinc finger protein family. Regions of the genome with zero ROH occurrences were also of major interest in specific populations. Depending on the breed, we detected between 2 to 57 no-ROH regions and identified 27 genes in these regions that were common for five breeds. These genes were involved in, e.g., muscle contractility ( CACNA1A ) and muscle development ( miR-23 , miR-24 , miR-27 ). To sum up, the obtained results can be furthered analyzed in the topic of identification of markers unique for specific horse breed characteristics.
- Published
- 2022
- Full Text
- View/download PDF
13. Another lesson from unmapped reads: in-depth analysis of RNA-Seq reads from various horse tissues.
- Author
-
Gurgul A, Szmatoła T, Ocłoń E, Jasielczuk I, Semik-Gurgul E, Finno CJ, Petersen JL, Bellone R, Hales EN, Ząbek T, Arent Z, Kotula-Balak M, and Bugno-Poniewierska M
- Subjects
- Animals, Base Sequence, Female, High-Throughput Nucleotide Sequencing, Horses genetics, Mammals genetics, RNA-Seq, Sequence Analysis, RNA, Transcriptome genetics, Genome genetics, RNA genetics
- Abstract
In recent years, a vast amount of sequencing data has been generated and large improvements have been made to reference genome sequences. Despite these advances, significant portions of reads still do not map to reference genomes and these reads have been considered as junk or artificial sequences. Recent studies have shown that these reads can be useful, e.g., for refining reference genomes or detecting contaminating microorganisms present in the analyzed biological samples. A special case of this is RNA sequencing (RNA-Seq) reads that come from tissue transcriptomes. Unmapped reads from RNA-Seq have received much less attention than those from whole-genome sequencing. In particular, in the horse, an analysis of unmapped RNA reads has not been performed yet. Thus, in this study, we analyzed the unmapped reads originating from the RNA-Seq performed through the Functional Annotation of Animal Genomes (FAANG) project in the horse, using eight different tissues from two mares. We demonstrated that unmapped reads from RNA-Seq could be easily assembled into transcripts relating to many important genes present in the sequences of other mammals. Large portions of these transcripts did not have coding potential and, thus, can be considered as non-coding RNA. Moreover, reads that were not mapped to the reference genome but aligned to the entries in NCBI database of horse proteins were enriched for biological processes that largely correspond to the functions of organ from which RNA was isolated and thus are presumably true transcripts of genes associated with cell metabolism in those tissues. In addition, a portion of reads aligned to the common pathogenic or neutral microbiota, of which the most common was Brucella spp. These data suggest that unmapped reads can be an important target for in-depth analysis that may substantially enrich results of initial RNA-Seq experiments for various tissues and organs., (© 2022. The Author(s), under exclusive licence to Institute of Plant Genetics Polish Academy of Sciences.)
- Published
- 2022
- Full Text
- View/download PDF
14. MicroRNA gene methylation landscape in pediatric B-cell precursor acute lymphoblastic leukemia.
- Author
-
Chaber R, Gurgul A, Tabarkiewicz J, Wróbel G, Szmatoła T, Jasielczuk I, Haus O, Lejman M, Rybka B, Ryczan-Krawczyk R, Jaśkowiec A, Paszek S, Potocka N, Arthur CJ, Bal W, Łach K, Kowal A, Zawlik I, and Latos-Grażyńska E
- Subjects
- Child, DNA Methylation, Humans, Methylation, Promoter Regions, Genetic, MicroRNAs genetics, Precursor B-Cell Lymphoblastic Leukemia-Lymphoma genetics, Precursor B-Cell Lymphoblastic Leukemia-Lymphoma pathology, Precursor Cell Lymphoblastic Leukemia-Lymphoma genetics
- Abstract
Background: Aberrant DNA methylation is an important mechanism by which the normal patterns of microRNA expression are disrupted in human cancers including B-cell precursor acute lymphoblastic leukemia (BCP ALL), the most common pediatric malignancy., Objectives: To characterize the methylation profile landscape of microRNA genes in BCP ALL patients., Material and Methods: We employed Infinium® MethylationEPIC BeadChip Arrays to measure the methylation of microRNA genes from bone marrow samples of children with BCP ALL (n = 38) and controls without neoplasms (n = 4)., Results: This analysis revealed differential methylation of the microRNA genes in the pediatric BCP ALL when compared to the control. A subcluster amongst BCP ALL patients with TCF3-PBX1 genetic subtype was also observed. No other differences were observed in association with age, gender or risk group. Several interesting leukemia-related phenotypes are enriched by the genes with hyperand hypomethylated sites located in promoters as well as gene bodies. The top 3 miRNA genes, promoters of which were the most statistically significantly hypermethylated in BCP ALL were MIR1273G, MIR1304 and MIR663, and the top 3 hypomethylated were MIR4442, MIR155 and MIR3909., Conclusions: In this study, a different microRNA genes methylation landscape was shown in pediatric BCP ALL compared to children without neoplasms. A visible subcluster among BCP ALL samples consisted of individuals with TCF3-PBX1 genetic subtype. No other differences were observed in association with age, gender or risk group. Several interesting leukemia-connected phenotypes were found, associated with genes with hyperand hypomethylated sites located on promoters as well as gene bodies.
- Published
- 2022
- Full Text
- View/download PDF
15. Pet ownership in pregnancy and methylation pattern in cord blood.
- Author
-
Danielewicz H, Gurgul A, Dębińska A, Myszczyszyn G, Szmatoła T, Myszkal A, Jasielczuk I, Drabik-Chamerska A, Hirnle L, and Boznański A
- Subjects
- DNA Methylation, Female, Glycosyltransferases, Humans, Mothers, Pregnancy, Transcription Factors metabolism, Fetal Blood metabolism, Ownership
- Abstract
Having pets in the house during the first years of life has been shown to protect against allergies. However, the result of different studies is heterogeneous. The aim of this study was to evaluate the methylation pattern in cord blood in relation to pet ownership during pregnancy.We investigated the methylation patterns of 96 cord blood samples, participants of the Epigenetic Hallmark of Maternal Atopy and Diet-ELMA project, born to mothers who either owned pets (n = 32) or did not own pets (n = 64) during their pregnancy. DNA from cord blood was analysed using the Infinium methylation EPIC. For statistical analysis, RnBeads software was applied.We found 113 differentially methylated sites (DMs) in the covariate-adjusted analysis (FDR p < 0.05), with small methylation differences. The top DMs were associated with genes: UBA7, THRAP3, GTDC1, PDE8A and SBK2. In the regional analysis, two promoter regions presented with significance: RN7SL621P and RNU6-211P. Cis-regulatory element analysis revealed significant associations with several immune-related pathways, such as regulation of IL18, Toll signalling, IL6 and complement.We conclude that pet exposure during pregnancy causes subtle but significant changes in methylation patterns in cord blood, which are reflected in the biological processes governing both innate and adaptive immune responses., (© 2021. The Author(s), under exclusive licence to Springer Nature Limited.)
- Published
- 2021
- Full Text
- View/download PDF
16. Maternal atopy and offspring epigenome-wide methylation signature.
- Author
-
Danielewicz H, Gurgul A, Dębińska A, Myszczyszyn G, Szmatoła T, Myszkal A, Jasielczuk I, Drabik-Chamerska A, Hirnle L, and Boznański A
- Subjects
- Adult, CpG Islands, Epigenesis, Genetic, Female, Fetal Blood metabolism, Genome-Wide Association Study, Humans, Maternal Exposure, Pregnancy, DNA Methylation, Epigenome
- Abstract
The increase in the prevalence of allergic diseases is believed to partially depend on environmental changes. DNA methylation is a major epigenetic mechanism, which is known to respond to environmental factors. A number of studies have revealed that patterns of DNA methylation may potentially predict allergic diseases.Here, we examined how maternal atopy is associated with methylation patterns in the cord blood of neonates.We conducted an epigenome-wide association study in a cohort of 96 mother-child pairs. Pregnant women aged not more than 35 years old, not currently smoking or exposed to environmental tobacco smoke, who did not report obesity before conception were considered eligible. They were further tested for atopy. Converted DNA from cord blood was analysed using Infinium MethylationEPIC; for statistical analysis, RnBeads software was applied. Gestational age and sex were included as covariates in the final analysis.83 DM sites were associated with maternal atopy. Within the top DM sites, there were CpG sites which mapped to genes SCD, ITM2C, NT5C3A and NPEPL1. Regional analysis revealed 25 tiling regions, 4 genes, 3 CpG islands and 5 gene promoters, (including PIGCP1, ADAM3A, ZSCAN12P1) associated with maternal atopy. Gene content analysis revealed pointwise enrichments in pathways related to purine-containing compound metabolism, the G1/S transition of the mitotic cell cycle, stem cell division and cellular glucose homoeostasis.These findings suggest that maternal atopy provides a unique intrauterine environment that may constitute the first environment in which exposure is associated with methylation patterns in newborn.
- Published
- 2021
- Full Text
- View/download PDF
17. A genome-wide association study for prolificacy in three Polish sheep breeds.
- Author
-
Smołucha G, Gurgul A, Jasielczuk I, Kawęcka A, and Miksza-Cybulska A
- Subjects
- Animals, Female, Genotype, Phenotype, Poland, Polymorphism, Single Nucleotide, Pregnancy, Receptor, EphA6 genetics, Genome-Wide Association Study veterinary, Litter Size, Sheep, Domestic genetics
- Abstract
Reproductive traits (especially litter size) are usually characterized by low heritability, and thus, phenotypic selection is often ineffective and slow. In order to improve fertility characteristics such as ovulation rate and litter size, it seems more effective to select breeding animals based on their genotype. The aim of the study was to use genome-wide association study (GWAS) in three sheep breeds to identify the genetic variants affecting the litter size in sheep. The study allowed us to identify one genome-wide significant SNP (rs402032081-located in ephrin type-A receptor 6, EPHA6) showing an association with litter size in Polish Mountain Sheep. We suggest that the EPHA6 gene can be a candidate gene for prolificacy trait in selected breeds of sheep; however, it needs further functional data for validation.
- Published
- 2021
- Full Text
- View/download PDF
18. Suitability of Pedigree Information and Genomic Methods for Analyzing Inbreeding of Polish Cold-Blooded Horses Covered by Conservation Programs.
- Author
-
Polak G, Gurgul A, Jasielczuk I, Szmatoła T, Krupiński J, and Bugno-Poniewierska M
- Subjects
- Animals, Conservation of Natural Resources, Genotype, Horses genetics, Pedigree, Polymorphism, Single Nucleotide, Genomics methods, Horses classification, Inbreeding methods
- Abstract
Traditionally, pedigree-based relationship coefficients were used to manage inbreeding and control inbreeding depression that occurs within populations. The extensive incorporation of genomic data in livestock breeding creates the opportunity to develop and implement methods to manage populations at the genomic level. Consequently, the realized proportion of the genome that two individuals share can be more accurately estimated instead of using pedigree information to estimate the expected proportion of shared alleles. To make use of this improvement, in this study we evaluated the genomic inbreeding measures in the Polish conserved cold-blooded horse population and compared the data with the traditional measures of inbreeding. Additionally, an ancestry fractions/proportions from Admixture software were tested as an estimate of lineage (ancestry coefficient) used for horses qualifying for the conservation program. The highest correlation of pedigree-based (F
PED ) and genomic inbreeding estimates was found for FROH (runs of homozygosity-based F coefficient) and FUNI (F coefficient based on the correlation between uniting gametes). FROH correlation with FPED tended to increase as the number of generations registered as pedigree increased. While lineage and gene contributions (Q) from Admixture software correlated, they showed poor direct compliance; hence, Q-value cannot be recommended as the estimate of pedigree-based lineage. All these findings suggest that the methods of genomics should be considered as an alternative or support in the analysis of population structure in conservative breeding that can help control inbreeding in rare horse populations.- Published
- 2021
- Full Text
- View/download PDF
19. Detection of runs of homozygosity in conserved and commercial pig breeds in Poland.
- Author
-
Szmatoła T, Jasielczuk I, Semik-Gurgul E, Szyndler-Nędza M, Blicharski T, Szulc K, Skrzypczak E, and Gurgul A
- Subjects
- Animals, Genotype, Homozygote, Inbreeding, Poland, Polymorphism, Single Nucleotide genetics, Swine, Breeding, Genome genetics, Genomics, Reproduction genetics
- Abstract
Runs of homozygosity (ROH) are continuous segments of the genome that arose as a result of inbreeding, resulting in the inheritance of identical haplotypes from both parents who shared a common ancestor. In the present study, we performed a detailed characterization and comparison of ROH in four pig breeds, including intensively selected Polish Landrace as well as native unselected animals of Puławska and two Złotnicka breeds (White and Spotted). We used a medium-density PorcineSNP60 BeadChip assay (Illumina) and cgaTOH software to detect ROH covering a minimum of 30 adjacent SNPs and maintaining a size over 1 Mb. By analysing ROH distribution and frequency across the genome, we also identified genomic regions with high ROH frequency (so-called "ROH hotspots"). The obtained results showed that the analysed conserved breeds were characterized by a higher ROH span and higher ROH-based inbreeding coefficients (F
ROH ), which likely result from past population bottlenecks, increasing the overall inbreeding level within these populations. The analysis of ROH distribution across the genomes revealed the presence of both shared and breed-specific ROH hotspots. These hotspots, presumably representing genome regions under selection, overlapped with a variety of genes associated with processes connected with immune system functioning, reproduction, glucose homeostasis and metabolism. The genome regions with ROH hotspots overlapping in all analysed populations, located on SSC4 (51.9-55.9 Mb) and 13 (92.6-97.8 Mb), covered thirty-one different genes, including MMP16, SLC7A13, ATP6V0D2, CNGB3, WWiP1, RiMDN1 and CPNE3. These genes are primarily associated with biological regulation and metabolism, processes that could be responsible for the variety of the selected production and functional features., (© 2020 Wiley-VCH GmbH.)- Published
- 2020
- Full Text
- View/download PDF
20. A detailed characteristics of bias associated with long runs of homozygosity identification based on medium density SNP microarrays.
- Author
-
Szmatoła T, Gurgul A, Jasielczuk I, Fu W, and Ropka-Molik K
- Abstract
In the present study, runs of homozygosity (ROH) detected with the use of a standard bovine 54k single nucleotide polymorphism (SNP) genotyping assay and two different ROH detection approaches, based on 50 (M1) or 15 (M2) consecutive SNPs, were compared with results of whole genome sequencing. Both microarray-based methods accurately recognised medium-sized ROH, however, it was found that M2 method seemed to better than M1 identify short ROH, but highly overestimated their number, leading to numerous false positive calls. Moreover, long ROH identified with microarray data tended to break into shorter segments in sequencing data because of the presence of regions with high heterozygosity within the ROH sequences. This may indicate, that these long ROH are formed by closely positioned shorter homozygous segments that may be of older origin or may be created by two similar but not identical haplotypes, showing minor internal recombination signs. Such finding also suggests that at least some of the results of previous studies in regard to long ROH may be biased leading to inaccurate estimations of genomes autozygosity via ROH classification into length categories., Competing Interests: Competing Interests: The authors have declared that no competing interest exists., (© The author(s).)
- Published
- 2020
- Full Text
- View/download PDF
21. Genetic Differentiation of the Two Types of Polish Cold-blooded Horses Included in the National Conservation Program.
- Author
-
Gurgul A, Jasielczuk I, Semik-Gurgul E, Pawlina-Tyszko K, Szmatoła T, Polak G, and Bugno-Poniewierska M
- Abstract
The current role of the horse as a companion animal resulted in a decrease of interest in breeding and usage of draft horses. This meant that the population of cold-blooded horses in Poland has been dramatically reduced during the last decades. To avoid impoverishment of the gene pool of the local horse population, a conservation program was established which involves draft horses and other local horse breeds. The draft horses bred in Poland can be subdivided in a few horse types of which the most widespread and consolidated are Sztumski and Sokólski horses. These two subpopulations are phenotypically diversified, however, the overall level of their genetic differentiation seems to be relatively low and not precisely determined, especially with the use of molecular markers. In reference to this, in this study we used Illumina genotyping arrays to describe in detail the genetic differentiation of these two cold-blooded horse populations. We describe the genetic distance between them, as well as within-population variation, admixture patterns, and level of relatedness within populations. We also made an attempt to detect genome regions divergently selected between those horses by the detection of diversifying selection signals. The results of this study provide initial evidence supporting breeding decisions that were made during conservation breeding program design and answer questions raised by the breeders of Sokólski and Sztumski horses concerning the level of their genetic variation and differentiation.
- Published
- 2020
- Full Text
- View/download PDF
22. A Comprehensive Analysis of Runs of Homozygosity of Eleven Cattle Breeds Representing Different Production Types.
- Author
-
Szmatoła T, Gurgul A, Jasielczuk I, Ząbek T, Ropka-Molik K, Litwińczuk Z, and Bugno-Poniewierska M
- Abstract
In the presented research, BovineSNP50 microarrays (Illumina) were applied to determine runs of homozygosity in the genomes of 11 cattle breeds maintained in Poland. These cattle breeds represent three basic utility types: milk, meat and dual purpose. Analysis of runs of homozygosity allowed the evaluation of the level of autozygosity within each breed in order to calculate the genomic inbreeding coefficient (F
ROH ), as well as to identify regions of the genome with a high frequency of ROH occurrence, which may reflect traces of directional selectin left in their genomes. Visible differences in the length and distribution of runs of homozygosity in the genomes of the analyzed cattle breeds have been observed. The highest mean number and mean sums of lengths of runs of homozygosity were characteristic for Hereford cattle and intermediate for the Holstein-Friesian Black-and-White variety, Holstein-Friesian Red-and-White variety, Simmental, Limousin, Montbeliarde and Charolais breeds. However, lower values were observed for cattle of conserved breeds. Moreover, the selected livestock differed in the level of inbreeding estimated using the FROH coefficient. In regions of the genome with a high frequency of ROH occurrence, which may reflect the impact of directional selection, a number of genes were observed that can be potentially related to the production traits which are under selection pressure for specific production types. The most important detected genes were GHR , MSTN , DGAT1 , FABP4 , and TRH , with a known influence on the milk and meat traits of the studied cattle breeds.- Published
- 2019
- Full Text
- View/download PDF
23. Induced androgenetic development in rainbow trout and transcriptome analysis of irradiated eggs.
- Author
-
Ocalewicz K, Gurgul A, Pawlina-Tyszko K, Szmatoła T, Jasielczuk I, Bugno-Poniewierska M, and Dobosz S
- Subjects
- Animals, Cell Nucleus genetics, Cell Nucleus radiation effects, Female, Gene Expression Profiling, Genes, Immediate-Early radiation effects, Haploidy, Male, Oncorhynchus mykiss growth & development, Ovum cytology, Ovum metabolism, RNA Stability radiation effects, RNA, Messenger, Stored metabolism, RNA, Messenger, Stored radiation effects, Transcriptome radiation effects, Aquaculture methods, Gene Expression Regulation, Developmental radiation effects, Oncorhynchus mykiss genetics, Ovum radiation effects, Paternal Inheritance radiation effects, Radiation, Ionizing
- Abstract
Ionizing radiation is administered to damage nuclear genome in fish eggs during induced androgenesis. In this study, we examined whether 350 Gy of X-ray applied to damage chromosomes in the rainbow trout eggs affects maternal RNA. Shortly after irradiation, we did not find any symptoms of RNA degradation in the treated eggs. Significant (p < 0.01) differences between non-irradiated and irradiated eggs concerned only a few transcripts including increased expression of immediate early response 2 (IER2) and early growth response 1 (EGR1) genes observed in the irradiated eggs. Both genes belong to the group of "immediate early genes" that respond quickly to the diverse extracellular stimuli. Elevated expression of these genes was accompanied by decreased level of ssa-miR-10b-5p and ssa-miR-21b-5p (p < 0.05), for which IER2 and EGR1 are target genes. The level of RNA in the fertilized irradiated eggs was highly significantly lower than in the non-irradiated eggs (p < 0.001) and in the unfertilized irradiated eggs (p < 0.0001). However, transcriptome profiles of fertilized non-irradiated eggs and fertilized irradiated eggs did not differ significantly. Thus, we assume that reduced abundance of mRNA in the fertilized irradiated eggs was associated with post-translational degradation and clearance of the maternal transcripts rather than from the irradiation of eggs.
- Published
- 2019
- Full Text
- View/download PDF
24. Genotyping-by-sequencing performance in selected livestock species.
- Author
-
Gurgul A, Miksza-Cybulska A, Szmatoła T, Jasielczuk I, Piestrzyńska-Kajtoch A, Fornal A, Semik-Gurgul E, and Bugno-Poniewierska M
- Subjects
- Animals, Breeding methods, Costs and Cost Analysis, Genotyping Techniques economics, Sequence Analysis, DNA economics, Genotyping Techniques methods, Livestock genetics, Polymorphism, Single Nucleotide, Sequence Analysis, DNA methods
- Abstract
Application of next generation sequencing for large scale genotyping in livestock is limited by high costs and challenging data analysis process. However, available restriction enzyme-based enrichment techniques like e.g. genotyping-by-sequencing (GBS) are promising tools allowing reduction of financial outlies by a high sample multiplexing and narrowing down the sequenced genome areas to the randomly distributed read tags. In this study, we tested the performance of standard, PstI endonuclease-adapted GBS protocol for population genetics in cattle, horse and sheep with application of different, including low-depth sequencing setups. It was found that the detected SNPs display desirable polymorphism parameters and are evenly scattered across the whole genome including gene coding regions. It was also shown that the SNPs can be successfully applied in population genetics, revealing the genetic differentiation of the studied breeds. The GBS approach represents a cost-effective alternative to existing genotyping methods which may find adoption in various research applications., (Copyright © 2018 Elsevier Inc. All rights reserved.)
- Published
- 2019
- Full Text
- View/download PDF
25. A genome-wide scan for diversifying selection signatures in selected horse breeds.
- Author
-
Gurgul A, Jasielczuk I, Semik-Gurgul E, Pawlina-Tyszko K, Stefaniuk-Szmukier M, Szmatoła T, Polak G, Tomczyk-Wrona I, and Bugno-Poniewierska M
- Subjects
- Animals, Chromosome Mapping, Female, Gene Frequency, Genetic Variation, Genetics, Population, Genome, Hair Color genetics, Horses anatomy & histology, Horses physiology, Male, Phenotype, Poland, Polymorphism, Single Nucleotide, Selective Breeding, Horses genetics
- Abstract
The genetic differentiation of the current horse population was evolutionarily created by natural or artificial selection which shaped the genomes of individual breeds in several unique ways. The availability of high throughput genotyping methods created the opportunity to study this genetic variation on a genome-wide level allowing detection of genome regions divergently selected between separate breeds as well as among different horse types sharing similar phenotypic features. In this study, we used the population differentiation index (FST) that is generally used for measuring locus-specific allele frequencies variation between populations, to detect selection signatures among six horse breeds maintained in Poland. These breeds can be classified into three major categories, including light, draft and primitive horses, selected mainly in terms of type (utility), exterior, performance, size, coat color and appearance. The analysis of the most pronounced selection signals found in this study allowed us to detect several genomic regions and genes connected with processes potentially important for breed phenotypic differentiation and associated with energy homeostasis during physical effort, heart functioning, fertility, disease resistance and motor coordination. Our results also confirmed previously described association of loci on ECA3 (spanning LCORL and NCAPG genes) and ECA11 (spanning LASP1 gene) with the regulation of body size in our draft and primitive (small size) horses. The efficiency of the applied FST-based approach was also confirmed by the identification of a robust selection signal in the blue dun colored Polish Konik horses at the locus of TBX3 gene, which was previously shown to be responsible for dun coat color dilution in other horse breeds. FST-based method showed to be efficient in detection of diversifying selection signatures in the analyzed horse breeds. Especially pronounced signals were observed at the loci responsible for fixed breed-specific features. Several candidate genes under selection were proposed in this study for traits selected in separate breeds and horse types, however, further functional and comparative studies are needed to confirm and explain their effect on the observed genetic diversity of the horse breeds., Competing Interests: The authors have declared that no competing interests exist.
- Published
- 2019
- Full Text
- View/download PDF
26. Corrigendum to "Transcriptome Analysis of Rainbow Trout ( Oncorhynchus mykiss ) Eggs Subjected to the High Hydrostatic Pressure Treatment".
- Author
-
Gurgul A, Pawlina-Tyszko K, Bugno-Poniewierska M, Szmatoła T, Jasielczuk I, Dobosz S, and Ocalewicz K
- Abstract
[This corrects the article DOI: 10.1155/2018/5197126.].
- Published
- 2018
- Full Text
- View/download PDF
27. A genome-wide detection of selection signatures in conserved and commercial pig breeds maintained in Poland.
- Author
-
Gurgul A, Jasielczuk I, Ropka-Molik K, Semik-Gurgul E, Pawlina-Tyszko K, Szmatoła T, Szyndler-Nędza M, Bugno-Poniewierska M, Blicharski T, Szulc K, Skrzypczak E, and Krupiński J
- Subjects
- Animals, Chromosome Mapping, Genotype, PPAR delta genetics, Phenotype, Poland, Polymorphism, Single Nucleotide, Principal Component Analysis, Swine, Selection, Genetic, Sus scrofa genetics
- Abstract
Background: Identification of selection signatures can provide a direct insight into the mechanism of artificial selection and allow further disclosure of the candidate genes related to the animals' phenotypic variation. Domestication and subsequent long-time selection have resulted in extensive phenotypic changes in domestic pigs, involving a number of traits, like behavior, body composition, disease resistance, reproduction and coat color. In this study, based on genotypes obtained from PorcineSNP60 Illumina assay we attempt to detect both diversifying and within-breed selection signatures in 530 pigs belonging to four breeds: Polish Landrace, Puławska, Złotnicka White and Złotnicka Spotted, of which the last three are a subject of conservative breeding and substantially represent the native populations., Results: A two largely complementary statistical methods were used for signatures detection, including: pairwise F
ST and relative extended haplotype homozygosity (REHH) test. Breed-specific diversifying selection signals included several genes involved in processes connected with fertility, growth and metabolism which are potentially responsible for different phenotypes of the studied breeds. The diversifying selection signals also comprised PPARD gene that was previously found to have a large effect on the shape of the external ear in pigs or two genes encoding neuropeptide Y receptors (Y2 and Y5) involved in fat deposition and stress response which are important features differentiating the studied breeds. REHH statistics allowed detecting several within-breed selection signatures overlapping with genes connected with a range of functions including, among others: metabolic pathways, immune system response or implantation and development of the embryo., Conclusions: The study provides many potential candidate genes with implication for traits selected in the individual breeds and gives strong basis for further studies aiming at identification of sources of variation among the studied pig breeds.- Published
- 2018
- Full Text
- View/download PDF
28. The distinguishable DNA whole genome methylation profile of 2 cases of pediatric precursor B acute lymphoblastic leukaemia (BCP ALL) with prodromal, preleukemic phase: A case report.
- Author
-
Chaber R, Gurgul A, Wróbel G, Tomoń A, Paszek S, Potocka N, Haus O, Lejman M, Łach K, Szmatoła T, Jasielczuk I, Rybka B, Ryczan-Krawczyk R, Stąpor S, Ciebiera K, Arthur CJ, and Zawlik I
- Subjects
- Acute Disease, Child, Humans, Male, Bone Marrow Cells metabolism, DNA Methylation, Precursor B-Cell Lymphoblastic Leukemia-Lymphoma genetics, Prodromal Symptoms
- Abstract
Rationale: A prolonged, prodromal phase before definitive paediatric precursor B acute lymphoblastic leukaemia (BCP ALL) diagnosis is rarely observed., Patients Concerns: In the first, the patient presented with an aplastic preleukemic phase, whilst the second presented with a rheumatic-like preliminary phase., Diagnoses: The case reports of two patients with BCP ALL with a prodromal phase lasting a few weeks are presented., Interventions and Outcomes: DNA whole genome profile methylation analysis of bone marrow cells obtained at diagnosis revealed a pattern of methylation that was readily distinguishable from both healthy and standard course BCP ALL bone marrow samples., Lessons: The biological implication of this observation remains unclear, with many differentially methylated loci involved in many processes like neurogenesis, cell projection organization and adhesion along with leucocyte activation and apoptosis. The prevalence and clinical significance of these methylation changes is unknown but this data indicates that the epigenetic basis of BCP ALL with a prolonged, prodromal phase requires a more detailed assessment.
- Published
- 2018
- Full Text
- View/download PDF
29. Transcriptome Analysis of Rainbow Trout ( Oncorhynchus mykiss ) Eggs Subjected to the High Hydrostatic Pressure Treatment.
- Author
-
Gurgul A, Pawlina-Tyszko K, Bugno-Poniewierska M, Szmatoła T, Jasielczuk I, Dobosz S, and Ocalewicz K
- Abstract
High hydrostatic pressure (HHP) causes depolymerization of the spindle microtubules. HHP applied to fish eggs prevents extrusion of the second polar body and inhibits the first cell cleavage, and it is used to produce triploids and diploid gynogenetic and androgenetic individuals. HHP has been also found to affect biomolecules including nucleic acids, and it may be presumed that HHP administered to the rainbow trout ( Oncorhynchus mykiss ) eggs disturbs cytoplasmic maternal RNA indispensable for the early embryogenesis. To verify this assumption, quality and quantity of RNA extracted from the rainbow trout eggs subjected to the high hydrostatic pressure shock were analyzed. Provided results exhibited that maternal transcriptome was resistant to a three-minute exposure to 65.5 MPa of HHP treatment. Some trend showing increase of the RNA integrity was observed in the HHP-treated eggs; however, the difference was not statistically significant. Alterations in the expression profiles in the rainbow trout eggs subjected to HHP were also negligible. Greater differences in the maternal gene expression were observed between eggs from different clutches than between HHP-treated and untreated eggs from the same clutch. It may be assumed that exposure to HHP shock was too short to modify significantly maternal transcripts in the rainbow trout eggs.
- Published
- 2018
- Full Text
- View/download PDF
30. Whole-genome DNA methylation characteristics in pediatric precursor B cell acute lymphoblastic leukemia (BCP ALL).
- Author
-
Chaber R, Gurgul A, Wróbel G, Haus O, Tomoń A, Kowalczyk J, Szmatoła T, Jasielczuk I, Rybka B, Ryczan-Krawczyk R, Duszeńko E, Stąpor S, Ciebiera K, Paszek S, Potocka N, Arthur CJ, and Zawlik I
- Subjects
- Adolescent, Age Factors, Child, Child, Preschool, CpG Islands, Female, Humans, Infant, Male, Promoter Regions, Genetic, Sex Factors, DNA Methylation, Precursor B-Cell Lymphoblastic Leukemia-Lymphoma genetics
- Abstract
In addition to genetic alterations, epigenetic abnormalities have been shown to underlie the pathogenesis of acute lymphoblastic leukemia (ALL)-the most common pediatric cancer. The purpose of this study was to characterize the whole genome DNA methylation profile in children with precursor B-cell ALL (BCP ALL) and to compare this profile with methylation observed in normal bone marrow samples. Additional efforts were made to correlate the observed methylation patterns with selected clinical features. We assessed DNA methylation from bone marrow samples obtained from 38 children with BCP ALL at the time of diagnosis along with 4 samples of normal bone marrow cells as controls using Infinium MethylationEPIC BeadChip Array. Patients were diagnosed and stratified into prognosis groups according to the BFM ALL IC 2009 protocol. The analysis of differentially methylated sites across the genome as well as promoter methylation profiles allowed clear separation of the leukemic and control samples into two clusters. 86.6% of the promoter-associated differentially methylated sites were hypermethylated in BCP ALL. Seven sites were found to correlate with the BFM ALL IC 2009 high risk group. Amongst these, one was located within the gene body of the MBP gene and another was within the promoter region- PSMF1 gene. Differentially methylated sites that were significantly related with subsets of patients with ETV6-RUNX1 fusion and hyperdiploidy. The analyzed translocations and change of genes' sequence context does not affect methylation and methylation seems not to be a mechanism for the regulation of expression of the resulting fusion genes.
- Published
- 2017
- Full Text
- View/download PDF
31. Shifts in rDNA levels act as a genome buffer promoting chromosome homeostasis.
- Author
-
Deregowska A, Adamczyk J, Kwiatkowska A, Gurgul A, Skoneczny M, Skoneczna A, Szmatola T, Jasielczuk I, Magda M, Rawska E, Pabian S, Panek A, Kaplan J, Lewinska A, and Wnuk M
- Subjects
- Cell Nucleolus drug effects, Cell Nucleolus metabolism, Chromosomes, Fungal metabolism, Comparative Genomic Hybridization, DNA Damage, DNA, Fungal metabolism, DNA, Ribosomal metabolism, Ethanol toxicity, Gene Expression Regulation, Fungal, Genomic Instability, Homeostasis, Oxidation-Reduction, Oxidative Stress, Saccharomyces cerevisiae drug effects, Saccharomyces cerevisiae growth & development, Saccharomyces cerevisiae metabolism, Selection, Genetic, Telomere genetics, Telomere metabolism, Cell Nucleolus genetics, Chromosomes, Fungal genetics, DNA, Fungal genetics, DNA, Ribosomal genetics, Genome, Fungal, Saccharomyces cerevisiae genetics
- Abstract
The nucleolus is considered to be a stress sensor and rDNA-based regulation of cellular senescence and longevity has been proposed. However, the role of rDNA in the maintenance of genome integrity has not been investigated in detail. Using genomically diverse industrial yeasts as a model and array-based comparative genomic hybridization (aCGH), we show that chromosome level may be balanced during passages and as a response to alcohol stress that may be associated with changes in rDNA pools. Generation- and ethanol-mediated changes in genes responsible for protein and DNA/RNA metabolism were revealed using next-generation sequencing. Links between redox homeostasis, DNA stability, and telomere and nucleolus states were also established. These results suggest that yeast genome is dynamic and chromosome homeostasis may be controlled by rDNA.
- Published
- 2015
- Full Text
- View/download PDF
32. General assessment of copy number variation in normal and tumor tissues of the domestic dog (Canis lupus familiaris).
- Author
-
Gurgul A, Żukowski K, Ślaska B, Semik E, Pawlina K, Ząbek T, Jasielczuk I, and Bugno-Poniewierska M
- Subjects
- Animals, Chromosome Mapping, Dogs, Female, Genome, Genomics, Male, Neoplasms genetics, Polymorphism, Single Nucleotide genetics, Biomarkers, Tumor genetics, DNA Copy Number Variations genetics, Dog Diseases genetics, Neoplasms veterinary
- Abstract
In recent years, characterization of a copy number variation (CNV) of the genomic DNA has provided evidence for the relationship of this type of genetic variation with the occurrence of a broad spectrum of diseases, including cancer lesions. Copy number variants (CNVs) also occur in the genomes of healthy individuals as a result of abnormal recombination processes in germ cells and have a hereditary character contributing to the natural genetic diversity. Recent image analysis methods and advanced computational techniques allow for identification of CNVs using SNPs genotyping microarrays based on the analysis of signal intensity observed for markers located in the specific genomic regions. In this study we used CanineHD BeadChip assay (Illumina) to identify both natural and cancer-induced CNVs in the genomes of different dog breeds and in different cancer types occurring in this species. The obtained results showed that structural aberrations are a common phenomenon arising during a tumor progression and are more complex and widespread in tumors of mesenchymal tissue origin than in epithelial tissue originating tumors. The tumor derived CNVs, in comparison to healthy samples, were characterized by larger sizes of regions, higher number of amplifications, and in some cases encompassed genes with potential effect on tumor progression.
- Published
- 2014
- Full Text
- View/download PDF
33. The application of genome-wide SNP genotyping methods in studies on livestock genomes.
- Author
-
Gurgul A, Semik E, Pawlina K, Szmatoła T, Jasielczuk I, and Bugno-Poniewierska M
- Subjects
- Animals, DNA Copy Number Variations genetics, Linkage Disequilibrium genetics, Genome genetics, Genotyping Techniques methods, Livestock genetics, Polymorphism, Single Nucleotide genetics
- Abstract
Animal genomics is currently undergoing dynamic development, which is driven by the flourishing of high-throughput genome analysis methods. Recently, a large number of animals has been genotyped with the use of whole-genome genotyping assays in the course of genomic selection programmes. The results of such genotyping can also be used for studies on different aspects of livestock genome functioning and diversity. In this article, we review the recent literature concentrating on various aspects of animal genomics, including studies on linkage disequilibrium, runs of homozygosity, selection signatures, copy number variation and genetic differentiation of animal populations. Our work is aimed at providing insight into certain achievements of animal genomics and to arouse interest in basic research on the complexity and structure of the genomes of livestock.
- Published
- 2014
- Full Text
- View/download PDF
Catalog
Discovery Service for Jio Institute Digital Library
For full access to our library's resources, please sign in.