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6. Prenatal programming by testosterone of follicular theca cell functions in ovary

Author

Monniaux, Danielle, primary, Genêt, Carine, additional, Maillard, Virginie, additional, Jarrier, Peggy, additional, Adriaensen, Hans, additional, Hennequet-Antier, Christelle, additional, Lainé, Anne-Lyse, additional, Laclie, Corinne, additional, Papillier, Pascal, additional, Plisson-Petit, Florence, additional, Estienne, Anthony, additional, Cognié, Juliette, additional, di Clemente, Nathalie, additional, Dalbies-Tran, Rozenn, additional, and Fabre, Stéphane, additional

Published
2019
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7. Regulation of AMH type 2 receptor expression by Bone Morphogenetic Protein 15 in ovine granulosa cells

Author

Estienne, Anthony, Pierre, Alice, Di Clemente, Nathalie, Racine, Chrystèle, Picard, Jean-Yves, Jarrier, Peggy, Lahoz, Belén, Alabart, José Luis, Folch, José, Fabre, Stéphane, Monniaux, Danielle, Physiologie de la reproduction et des comportements [Nouzilly] (PRC), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours-Centre National de la Recherche Scientifique (CNRS), Physiologie de l'Axe Gonadotrope (PAG U1133), Unité de Biologie Fonctionnelle et Adaptative (BFA (UMR_8251 / U1133)), Université Paris Diderot - Paris 7 (UPD7)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université Paris Diderot - Paris 7 (UPD7)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Centro de Investigacion y Tecnologia Agroalimentaria de Aragon (CITA), Génétique Physiologie et Systèmes d'Elevage (GenPhySE ), École nationale supérieure agronomique de Toulouse [ENSAT]-Institut National de la Recherche Agronomique (INRA)-Ecole Nationale Vétérinaire de Toulouse (ENVT), Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées, International Organisation on Animal Reproduction. FRA., and Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS)

Subjects
[SDV.OT]Life Sciences [q-bio]/Other [q-bio.OT], ComputingMilieux_MISCELLANEOUS
Abstract

National audience

Published
2016

8. Differential gene expression from preantral to antral follicles in vivo

Author

Cadoret, Véronique, Frapsauce, Cynthia, Jarrier, Peggy, Maillard, Virginie, Bonnet, Agnès, Locatelli, Yann, Royère, Dominique, Monget, Philippe, Monniaux, Danielle, Guerif, Fabrice, Physiologie de la reproduction et des comportements [Nouzilly] (PRC), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours-Centre National de la Recherche Scientifique (CNRS), Génétique Physiologie et Systèmes d'Elevage (GenPhySE ), École nationale supérieure agronomique de Toulouse [ENSAT]-Institut National de la Recherche Agronomique (INRA)-Ecole Nationale Vétérinaire de Toulouse (ENVT), Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées, INRA, Région Centre, International Organisation on Animal Reproduction. FRA., and Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS)

Subjects
[SDV.OT]Life Sciences [q-bio]/Other [q-bio.OT], ComputingMilieux_MISCELLANEOUS
Abstract

National audience

Published
2016

9. KO d’un gène OvocytAire chez le Lapin (KOALA)

Author

Dalbies-Tran, Rozenn, Jarrier, Peggy, Beauclair, Linda, Peyny, Maud, Guitton, Edouard, Boulanger, Laurent, Daniel, Nathalie, Duranthon, Véronique, Physiologie de la reproduction et des comportements [Nouzilly] (PRC), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS), Plateforme d'Infectiologie Expérimentale (PFIE), Institut National de la Recherche Agronomique (INRA), Biologie du Développement et Reproduction (BDR), École nationale vétérinaire d'Alfort (ENVA)-Institut National de la Recherche Agronomique (INRA), Institut National de Recherche Agronomique (INRA). UAR Département Physiologie Animale et Systèmes d'Elevage (0558)., Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours-Centre National de la Recherche Scientifique (CNRS), and ProdInra, Migration

Subjects
talen, embryon, gène, [SDV.BDD] Life Sciences [q-bio]/Development Biology, bcar4, biotechnologies, ovocyte, [SDV.BDD]Life Sciences [q-bio]/Development Biology, ComputingMilieux_MISCELLANEOUS, effet maternel
Abstract

National audience

Published
2016

10. Regulation of AMH type 2 receptor expression by Bone Morphogenetic Protein 15 in ovine granulosa cells (abstract)

Author

Estienne, Anthony, Pierre, Alice, Di Clemente, Nathalie, Racine, Chrystèle, Picard, Jean-Yves, Jarrier, Peggy, Lahoz Crespo, Belén, Alabart Alvarez, José Luis, Fabre, Stéphane, and Monniaux, Danielle

Subjects
producción y sanidad animal, Reproductividad, Hormonas, GeneralLiterature_REFERENCE(e.g.,dictionaries,encyclopedias,glossaries), Ovinos
Abstract

Published

Published
2016

13. Culture in vitro de follicules préantraux: Un modèle de développement coordonné du follicule et de l’ovocyte

Author

Cadoret, Véronique, Jarrier, Peggy, Maillard, Virginie, Bonnet, Agnès, Locatelli, Yann, Monget, Philippe, Monniaux, Danielle, Royère, Dominique, Guerif, Fabrice, Frapsauce, Cynthia, Physiologie de la reproduction et des comportements [Nouzilly] (PRC), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS), Laboratoire de Biologie de la Reproduction, Centre Hospitalier Régional Universitaire de Tours (CHRU Tours), Génétique Physiologie et Systèmes d'Elevage (GenPhySE ), École nationale supérieure agronomique de Toulouse [ENSAT]-Institut National de la Recherche Agronomique (INRA)-Ecole Nationale Vétérinaire de Toulouse (ENVT), Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées, Direction des Jardins botaniques et zoologiques, Muséum national d'Histoire naturelle (MNHN), INRA, Région Centre Val de Loire, Fédération Française d'Etude de la Reproduction (FFER). FRA., Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours-Centre National de la Recherche Scientifique (CNRS), and Centre Hospitalier Régional Universitaire de Tours (CHRU TOURS)

Subjects
[SDV.BDLR]Life Sciences [q-bio]/Reproductive Biology, ComputingMilieux_MISCELLANEOUS
Abstract

National audience

Published
2015

14. Development of preantral follicles in culture: Cellular characterization and gene expression

Author

Cadoret, Véronique, Frapsauce, Cynthia, Jarrier, Peggy, Maillard, Virginie, Bonnet, Agnès, Roulet, Alain, Monget, Philippe, Monniaux, Danielle, Royère, Dominique, Locatelli, Yann, Guerif, Fabrice, Physiologie de la reproduction et des comportements [Nouzilly] (PRC), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS), Laboratoire de Biologie de la Reproduction, Centre Hospitalier Régional Universitaire de Tours (CHRU Tours), Génétique Physiologie et Systèmes d'Elevage (GenPhySE ), École nationale supérieure agronomique de Toulouse [ENSAT]-Institut National de la Recherche Agronomique (INRA)-Ecole Nationale Vétérinaire de Toulouse (ENVT), Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées, Département de Génétique Animale [Toulouse], Institut National de la Recherche Agronomique (INRA), Direction des Jardins botaniques et zoologiques, Muséum national d'Histoire naturelle (MNHN), INRA, Région Centre, Université de Haute Bretagne ( Rennes 2 ) (UR 2). Rennes, FRA., Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours-Centre National de la Recherche Scientifique (CNRS), and Centre Hospitalier Régional Universitaire de Tours (CHRU TOURS)

Subjects
[SDV.OT]Life Sciences [q-bio]/Other [q-bio.OT], ComputingMilieux_MISCELLANEOUS
Abstract

National audience

Published
2015

16. The Bone Morphogenetic Protein 15 Up-Regulates the Anti-Müllerian Hormone Receptor Expression in Granulosa Cells

Author

Pierre, Alice, primary, Estienne, Anthony, additional, Racine, Chrystèle, additional, Picard, Jean-Yves, additional, Fanchin, Renato, additional, Lahoz, Belén, additional, Alabart, José Luis, additional, Folch, José, additional, Jarrier, Peggy, additional, Fabre, Stéphane, additional, Monniaux, Danielle, additional, and di Clemente, Nathalie, additional

Published
2016
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17. Involvement of the bone morphogenetic protein receptor type I in anti-Müllerian hormone regulation in the sheep ovary

Author

Estienne, Anthony, Monniaux, Danielle, Jarrier, Peggy, Fabre, Stéphane, Physiologie de la reproduction et des comportements [Nouzilly] (PRC), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS), Laboratoire de Génétique Cellulaire (LGC), Institut National de la Recherche Agronomique (INRA)-Ecole Nationale Vétérinaire de Toulouse (ENVT), Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées, ANR Programs of the French National Research Agency (Genovul, Monopoly and Amharoc, INRA, Région Centre, Society for the Study of Reproduction (SSR). Madison, WI, USA., and Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours-Centre National de la Recherche Scientifique (CNRS)

Subjects
[SDV.OT]Life Sciences [q-bio]/Other [q-bio.OT], ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
2013

19. Regulation of anti-mullerian hormone production in the cow: a multiscale study at endocrine, ovarian, follicular, and granulosa cell levels

Author

Rico, Charlène, Médigue, Claire, Fabre, Stéphane, Jarrier, Peggy, Bontoux, Martine, Clément, Frédérique, Monniaux, Danielle, Physiologie de la reproduction et des comportements [Nouzilly] (PRC), Centre National de la Recherche Scientifique (CNRS)-Université de Tours-Institut Français du Cheval et de l'Equitation [Saumur]-Institut National de la Recherche Agronomique (INRA), SIgnals and SYstems in PHysiology & Engineering (SISYPHE), Inria Paris-Rocquencourt, Institut National de Recherche en Informatique et en Automatique (Inria)-Institut National de Recherche en Informatique et en Automatique (Inria), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur] (IFCE)-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS), This work was supported by specific funding (Crédits Fléchés) of the INRA PHASE division, by the REGATE INRIA/INRA Large Scale Initiative Action, by the INRA/DGER PREDICTOV development project, and by a French fellowship from the Région Centre to C.R., Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS), and Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours-Centre National de la Recherche Scientifique (CNRS)

Subjects
Anti-Mullerian Hormone, endocrine system, endocrine system diseases, anti mullerian hormone, follicular development, Endocrine System, Estrous Cycle, follicle, Ovarian Follicle, Animals, mullerian inhibiting substance, multiple ovulation and embryo transfer, Cells, Cultured, Progesterone, Cell Size, superovulation, Granulosa Cells, Estradiol, urogenital system, Ovary, granulosa cell, [SDV.BIBS]Life Sciences [q-bio]/Quantitative Methods [q-bio.QM], female genital diseases and pregnancy complications, Gene Expression Regulation, bmp, Cattle, Female, [INFO.INFO-BI]Computer Science [cs]/Bioinformatics [q-bio.QM], hormones, hormone substitutes, and hormone antagonists
Abstract

Chantier qualité GA; International audience; Anti-Müllerian hormone (AMH) is an endocrine marker that can help predict superovulatory responses to treatments administered to cows for embryo production. However, the optimal time of the estrous cycle at which a blood test should be performed for a highly reliable prognosis has not yet been established. Moreover, little is known about the regulation of AMH production. To answer these questions, a study was designed to investigate the regulation of AMH production in cows selected for their high or low ovulatory responses to superovulation. At the granulosa cell level, AMH production was inhibited by follicle-stimulating hormone but enhanced by bone morphogenetic proteins. At the follicular level, the expression of AMH within the follicle was dependent on the stage of follicular development. At the ovarian level, the size of the pool of small antral growing follicles determined ovarian AMH production. At the endocrine level, AMH followed a specific dynamic profile during the estrous cycle, which occurred independently of the follicular waves of terminal follicular development. Cows selected for their high or low responses to superovulation did not differ in the regulation of AMH production, but cows with higher responses had higher plasma AMH concentrations throughout the cycle. The optimal period of the estrous cycle at which to measure AMH concentrations with the aim of selecting the best cows for embryo production was found to be at estrus and after Day 12 of the cycle. Based on this multiscale study, we propose a model that integrates the different regulatory levels of AMH production.

Published
2011
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20. Regulation of anti-Müllerian hormone production in domestic animals

Author

Monniaux, Danielle, primary, Drouilhet, Laurence, additional, Rico, Charlène, additional, Estienne, Anthony, additional, Jarrier, Peggy, additional, Touzé, Jean-Luc, additional, Sapa, Jean, additional, Phocas, Florence, additional, Dupont, Joëlle, additional, Dalbiès-Tran, Rozenn, additional, and Fabre, Stéphane, additional

Published
2013
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21. Determination of anti-Müllerian hormone concentrations in blood as a tool to select Holstein donor cows for embryo production: from the laboratory to the farm

Author

Rico, Charlène, primary, Drouilhet, Laurence, additional, Salvetti, Pascal, additional, Dalbiès-Tran, Rozenn, additional, Jarrier, Peggy, additional, Touzé, Jean-Luc, additional, Pillet, Elodie, additional, Ponsart, Claire, additional, Fabre, Stéphane, additional, and Monniaux, Danielle, additional

Published
2012
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31. Regulation of anti-Müllerian hormone production in domestic animals.

Author

Monniaux, Danielle, Drouilhet, Laurence, Rico, Charlène, Estienne, Anthony, Jarrier, Peggy, Touzé, Jean-Luc, Sapa, Jean, Phocas, Florence, Dupont, Joëlle, Dalbiès-Tran, Rozenn, and Fabre, Stéphane

Subjects
ANTI-Mullerian hormone, BONE morphogenetic proteins, DOMESTIC animals, GRANULOSA cells, GONADOTROPIN, RUMINANTS
Abstract

In mammals, anti-Müllerian hormone (AMH) expression is detected in the granulosa cells of all growing follicles and is highest in healthy small antral follicles, which contribute most significantly to AMH endocrine levels. AMH is a reliable endocrine marker of this population of gonadotrophin-responsive follicles in ruminants and, over the longer term, plasma AMH concentrations are characteristic of individual animals. In the cow, plasma AMH concentrations follow specific dynamic profiles throughout the prepubertal period, the oestrous cycle and the change from gestation to the post partum period, with the alterations most likely reflecting numerical changes in the population of high AMH-producing follicles. In granulosa cells, bone morphogenetic proteins (BMP) enhance AMH gene expression and AMH synthesis, with these effects antagonised by FSH. BMP could both support follicular growth and contribute significantly to the induction and/or maintenance of AMH expression in small growing follicles. AMH expression decreases sharply in large follicles when they become oestrogenic, suggesting a role for FSH and/or oestradiol in these changes, but the underlying mechanisms remain hypothetical. A better understanding of the factors and mechanisms regulating AMH production is needed to propose new strategies for managing the reserve of primordial and small growing follicles, as well as for improving embryo production. [ABSTRACT FROM AUTHOR]

Published
2012
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32. Identification of CXCR4 as a New Nitric Oxide-Regulated Gene in Human CD34+ Cells.

Author

YANYAN ZHANG, WITTNER, MONIKA, BOUAMAR, HAKIM, JARRIER, PEGGY, VAINCHENKER, WILLIAM, and LOUACHE, FAWZIA

Subjects
NITRIC oxide, CHEMOKINES, CELLS, GENE expression, CELL migration
Abstract

The article reports on the identification of chemokine receptor CXCR4 as a new nitric oxide (NO)-regulated gene in human CD34+ cells. It was found that NO pathway can modulate CXCR4 expression in CD34+ cells. CD34+ cells treated with NO donors exhibited an increased chemotactic response. The results also suggest that NO may plan a critical role in the trafficking of hematopoietic progenitors.

Published
2007
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34. p210BCR-ABLby down Regulating FLI-1 Reprograms Megakaryocytic Progenitors to the Erythroid Lineage.

Author

Buet, Dorothée, Raslova, Hana, Juban, Gaëtan, Geay, Jean-François, Jarrier, Peggy, Turhan, Ali G., Morle, François, Vainchenker, William, and Louache, Fawzia

Abstract

Blood cells derive from a common multipotent stem cell that undergoes successive commitment steps with progressive restriction in differentiation ability. A close developmental relationship exists between the erythroid and megakaryocytic (MK) lineages as both lineages derive from a common bipotent progenitor. The nature of the events governing erythroid-specific or MK-specific programming is yet poorly understood. To approach this issue, we used an ectopic expression of the p210 BCR-ABLoncoprotein in adult primary human CD34+progenitor cells. In culture conditions favoring the development of both erythroid and MK cells, we observed a major expansion of the erythroid lineage occurring at the expense of the MK lineage. The combined use of a p210BCR-ABLtyrosine kinase inhibitor together with single cell cloning experiments on prospectively isolated CD41+CD42−progenitor cells allowed us to identify that a part of MK progenitor cells were reprogrammed by p210BCR-ABLto take an erythroid fate. A similar lineage switching was seen after p210BCR-ABLexpression in the human megakaryoblastic MO7e cell line. Microarrays and semi-quantitative RT-PCR studies showed that expression of p210BCR-ABLwas associated with a decreased expression of the FLI-1 transcription factor. Moreover, re-expression of FLI-1 in p210BCR-ABL-transduced MO7e cells that had acquired an erythroid phenotype rescued the MK programming. Finally, a partial inhibition of FLI-1 in normal CD41+CD42−progenitor cells with a shRNAi led to an increased erythroid differentiation at the expense of MK differentiation. These results demonstrate that progenitor cells apparently committed to the MK lineage may be re-specified to the erythroid lineage by an ectopic expression of p210BCR-ABLand that the FLI-1 transcription factor is a key factor in the balance between erythroid versus MK commitment.

Published
2005
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35. Regulation of anti-Müllerian hormone production in the cow: a multiscale study at endocrine, ovarian, follicular, and granulosa cell levels.

Author

Rico C, Médigue C, Fabre S, Jarrier P, Bontoux M, Clément F, and Monniaux D

Subjects
Animals, Cattle, Cell Size, Cells, Cultured, Endocrine System metabolism, Endocrine System physiology, Estradiol blood, Estrous Cycle blood, Estrous Cycle genetics, Estrous Cycle metabolism, Female, Gene Expression Regulation, Granulosa Cells cytology, Ovarian Follicle cytology, Progesterone blood, Anti-Mullerian Hormone genetics, Anti-Mullerian Hormone metabolism, Granulosa Cells metabolism, Ovarian Follicle metabolism, Ovary metabolism
Abstract

Anti-Müllerian hormone (AMH) is an endocrine marker that can help predict superovulatory responses to treatments administered to cows for embryo production. However, the optimal time of the estrous cycle at which a blood test should be performed for a highly reliable prognosis has not yet been established. Moreover, little is known about the regulation of AMH production. To answer these questions, a study was designed to investigate the regulation of AMH production in cows selected for their high or low ovulatory responses to superovulation. At the granulosa cell level, AMH production was inhibited by follicle-stimulating hormone but enhanced by bone morphogenetic proteins. At the follicular level, the expression of AMH within the follicle was dependent on the stage of follicular development. At the ovarian level, the size of the pool of small antral growing follicles determined ovarian AMH production. At the endocrine level, AMH followed a specific dynamic profile during the estrous cycle, which occurred independently of the follicular waves of terminal follicular development. Cows selected for their high or low responses to superovulation did not differ in the regulation of AMH production, but cows with higher responses had higher plasma AMH concentrations throughout the cycle. The optimal period of the estrous cycle at which to measure AMH concentrations with the aim of selecting the best cows for embryo production was found to be at estrus and after Day 12 of the cycle. Based on this multiscale study, we propose a model that integrates the different regulatory levels of AMH production.

Published
2011
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36. Identification of CXCR4 as a new nitric oxide-regulated gene in human CD34+ cells.

Author

Zhang Y, Wittner M, Bouamar H, Jarrier P, Vainchenker W, and Louache F

Subjects
Cell Culture Techniques, Cell Survival drug effects, DNA Primers, Fetal Blood cytology, Flow Cytometry, Gene Expression Regulation drug effects, Hematopoietic Stem Cells drug effects, Humans, Infant, Newborn, Kinetics, Nitroso Compounds pharmacology, Polymerase Chain Reaction, RNA, Messenger genetics, Transcription, Genetic drug effects, Antigens, CD34 analysis, Gene Expression Regulation physiology, Hematopoietic Stem Cells physiology, Nitric Oxide pharmacology, Nitric Oxide Donors pharmacology, Receptors, CXCR4 genetics
Abstract

As an intracellular second messenger, nitric oxide (NO) is increasingly implicated in the control of transcriptional machinery and gene expression. Here, we show that cell surface expression of CXCR4 on CD34(+) cells was increased in a dose- and time-dependent manner in response to NO donors. Augmented surface expression was correlated with an increase in CXCR4 mRNA level. A specific NO scavenger prevented the elevation in CXCR4 mRNA caused by NO donors, suggesting a direct signaling action mediated by NO on CXCR4 transcription. NO treatment had no significant effect on CXCR4 mRNA stability. However, induction of CXCR4 mRNA by NO was still observed in conditions in which initiation of translation was inhibited, suggesting that the NO effect must be mediated by a pre-existing protein. CXCR4 mRNA induction did not involve cGMP (guanosine 3', 5'-cyclic monophosphate) generation but was most likely mediated via oxidation of intracellular protein thiols. Finally, CD34(+) cells pretreated with NO donors exhibited an increased chemotactic response. This study demonstrates that the NO pathway can modulate CXCR4 expression in human CD34(+) cells and suggests that NO may play a critical role in the trafficking of hematopoietic progenitors.

Published
2007
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37. Intracellular localization and constitutive endocytosis of CXCR4 in human CD34+ hematopoietic progenitor cells.

Author

Zhang Y, Foudi A, Geay JF, Berthebaud M, Buet D, Jarrier P, Jalil A, Vainchenker W, and Louache F

Subjects
Antigens, CD biosynthesis, Cell Membrane metabolism, Cell Movement, Chemotaxis, Clathrin metabolism, Endocytosis, Flow Cytometry, Gene Expression Regulation, Green Fluorescent Proteins metabolism, Hematopoietic Stem Cells metabolism, Humans, Ligands, Lysosomal Membrane Proteins, Membrane Proteins biosynthesis, Microscopy, Confocal, Plasmids metabolism, Platelet Membrane Glycoproteins biosynthesis, Retroviridae genetics, Signal Transduction, Temperature, Tetraspanin 30, Vesicular Transport Proteins, rab GTP-Binding Proteins biosynthesis, rab4 GTP-Binding Proteins biosynthesis, rab5 GTP-Binding Proteins biosynthesis, Antigens, CD34 biosynthesis, Hematopoietic Stem Cells cytology, Receptors, CXCR4 biosynthesis
Abstract

CXCR4, the stromal cell-derived factor-1 receptor, plays an important role in the migration of hematopoietic progenitor/stem cells. The surface and cytoplasmic expression of CXCR4 on human hematopoietic CD34(+) cells was investigated. We show that its surface expression is low, whereas a large part of CXCR4 protein is sequestered intracellularly. Using confocal microscopy, we demonstrated that CXCR4 is colocalized with EEA-1, Rab5, Rab4, and Rab11, which are localized in early and recycling endosomes. No significant colocalization of CXCR4 with lysosomal markers CD63 and Lamp-1 was detected. Using antibody feeding experiments, we report a role for CXCR4 constitutive endocytosis in subcellular localization in stably transduced UT7-CXCR4-GFP and CD34(+) cells. Agonist-independent endocytosis of CXCR4 occurs through clathrin-coated vesicles. These data implicate a constitutive endocytosis in the regulation of CXCR4 membrane expression and suggest that constitutive endocytosis may be involved in the regulation of trafficking the human hematopoietic progenitor/stem cells to and in the bone marrow microenvironment.

Published
2004
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