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The aim of this study was to determine the effect of gradient static magnetic field (SMF) on reactive oxygen species (ROS) production in human neutrophils in peripheral blood in vitro. Blood samples collected from healthy individuals were incubated in an inhomogeneous SMF (in a south or north pole of the field) for 15, 30 or 45 minutes. The maximum value of induction (B max) amounted to ≈ 60 mT. To determine the strength of the ROS production, dihydrorhodamine (123DHR) as fluorophore and phorbol 12-myristate 13-acetate (PMA) as respiratory burst stimulator were used. 123DHR oxidation by ROS was measured by flow cytometry. The exposure of blood samples to SMF induced statistically significant changes in ROS production in unstimulated and PMA-stimulated neutrophils. The observed effects were highly correlated with the exposure time and depended on the orientation of the field. Although intracellular mechanisms underlying such interactions are not thoroughly understood, it could be presumed that SMF affects ROS metabolic oscillations and their formation and inactivation. This study emphasizes the importance of proper adjustment of exposure time to SMF for any potential therapeutic applications.

The present study was undertaken in order to determine the effect of low frequency electromagnetic field (EMF) on reactive oxygen species (ROS) production in human neutrophils in peripheral blood in vitro. We investigated how differently generated EMF and several levels of magnetic induction affect ROS production. To evaluate the level of ROS production, two fluorescent dyes were used: 2'7'-dichlorofluorscein-diacetate and dihydrorhodamine. Phorbol 12-myristate 13-acetate (PMA), known as strong stimulator of the respiratory burst, was also used. Alternating magnetic field was generated by means of Viofor JPS apparatus. Three different levels of magnetic induction have been analyzed (10, 40 and 60 μT). Fluorescence of dichlorofluorescein and 123 rhodamine was measured by flow cytometry. The experiments demonstrated that only EMF tuned to the calcium ion cyclotron resonance frequency was able to affect ROS production in neutrophils. Statistical analysis showed that this effect depended on magnetic induction value of applied EMF. Incubation in EMF inhibited cell activity slightly in unstimulated neutrophils, whereas the activity of PMA-stimulated neutrophils has increased after incubation in EMF.

Quantitative analysis of blood viscosity was performed on the basis of mathematical models of non-Newtonian fluid shear flow behavior (Casson, Ree-Eyring and Quemada). A total of 100 blood samples were drawn from clinically stable survivors of myocardial infarction, treated with aspirin or acenocoumarol and controls to these drugs. Whole blood and plasma viscosity were measured at a broad range of shear rates using a rotary-oscillating viscometer Contraves LS40. Numerical analysis of the experimental data was carried out by means of linear (for Casson) and non-linear regression for the remaining models. In the evaluation of the results, both the fit quality and physical interpretation of the models' parameters were considered. The Quemada model fitted most precisely with the experimental findings and, despite the controversies concerning the relationship between in vivo tissue perfusion and in vitro rheological measurements, seemed to be a valuable method enhancing investigation possibilities of cardiovascular patients. Our results suggest that aspirin does not affect blood rheological properties, while acenocoumarol may slightly alter red cell deformability and rouleaux formation.

Determination of temperature dependencies of electric conductivity and thermal properties by differential scanning calorimetry (DSC) allow to analyse the processes of charge and heat transport in the bone being a complex collagen-hydroxyapatite (HAP)-water system. Modification of the bone structure by high doses of gamma-radiation changes the electrical and thermal properties of the bone. Electrical conductivity (sigma) of the bone decreases with consecutive heating runs. The decrease in sigma observed for irradiated samples was explained by the scission of the main chain of collagen macromolecule. Irradiation decreased the hydration level in the bone, its denaturation temperature and increased both enthalpy and entropy of the denaturation process.

The fully automatic method for reconstruction of spermatozoa trajectories was presented. The method is based on computer image analysis. It allow to process pure semen without any treatment like diluting or purifying. Since no human interacting is available, this method lead to objective results. The method was used to analyse semen taken from healthy men (WHO 92 standard). The result shows absence of different populations in single sample.

The force needed to press the key in a simple reaction time task was measured as a function of stimulus intensity for visual and auditory stimuli in three experiments using a total 45 male and female human subjects. Intensity ranged from 0.316 to 1995 cd/m2 for visual stimuli and from ranged from 47 to 102 dB for auditory stimuli. We found, in agreement with Angel's (1973) original study, that for auditory stimuli higher intensity is accompanied by a larger force. Surprisingly, in the case of visual stimuli the intensity does not influence the force. These findings are explained by the assumption that the changes of force reflect the changes of unspecific activation level evoked by immediate arousal. Thus, the different behaviour of force for these two modalities is in agreement with the common view that loud auditory stimuli are arousing while intense visual ones are not.

The effects of loudness on the latency of evoked potentials and on simple reaction time were compared. It was found that both reaction time and the evoked-potential latency increases with decreasing stimulus intensity. However, different slopes of the curves were found. This is explained in terms of the arousal effect of loud auditory stimuli.

Reaction times and the relative latency evaluated by the temporal-order-judgment method for two stimuli of different modalities (visual and auditory) were measured. The difference between reaction times for visual and auditory stimuli was about 40 ms. The relative latency was slightly shorter, however; in conflict with Rutschmann and Link's (1964) previous result, the auditory stimulus must be delayed to be perceived simultaneously with the visual one.

Dielectric hysteresis loop and differential thermal analysis (DTA) studies of collagen were performed for temperatures ranging from a room temperature to 393 K. The material studied was collagen from Achilles tendon of a bullock, which contained 10% of water. The DTA curve for collagen with 10% of water showed a clear minimum at 353 K. The character of the curve proved that around 353 K an endothermic transformation of collagen took place which could be connected with the transition spiral-ball. A hypothetical dielectric hysteresis loop shows a maximum shift along the direction of the electric field applied in the vicinity of 375 K. Low frequencies of the applied electric field are connected with a great dielectric loss influencing the interpretation of collagen ferroelectricity.