The study was conducted to address the nutritional analysis of juice and citric acid content, total soluble solids, and sugar concentration among theselected acidic and sub acidic citrus fruits, which are available locally in Robe Town, South Eastern Ethiopia. Four acidic and sub acidic citrus fruit samples including Sweet lemon; Lime, Pineapple, and Mango were collected and analyzed through different analytical methods on the same day. The obtained results indicated that the % of juice content high in sweet lemon than other fruits, being an acidic fruit lime contained the highest concentration of citric acid as compared to other fruits. But the contents of Mango and Sweet lime were found to be highly rich in total soluble solids and sugar content than other fruits. On the other hand, Sweet lemon and ineapple have possessed sugar concentration. Hence, the present study concluded based on observations, each fruit has specific characteristics having high nutritional value and the daily intake of fruits enhances the immunity system to defend against many diseases.