166 results on '"Jantrawut P"'
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2. Determination of vat-photopolymerization parameters for microneedles fabrication and characterization of HPMC/PVP K90 dissolving microneedles utilizing 3D-printed mold
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Baramee Chanabodeechalermrung, Tanpong Chaiwarit, Suruk Udomsom, Pornchai Rachtanapun, Promporn Piboon, and Pensak Jantrawut
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Medicine ,Science - Abstract
Abstract Three-dimensional (3D) printing serves as an alternative method for fabricating microneedle (MN) patches with a high object resolution. In this investigation, four distinct needle shapes: pyramid mounted over a long cube (shape A), cone mounted over a cylinder (shape B), pyramidal shape (shape C), and conical shape (shape D) were designed using computer-aided design (CAD) software with compensated bases of 350, 450 and 550 µm. Polylactic acid (PLA) biophotopolymer resin from eSun and stereolithography (SLA) 3D printer from Anycubic technology were used to print MN patches. The 3D-printed MN patches were employed to construct MN molds, and those molds were used to produce hydroxypropyl methylcellulose (HPMC) and polyvinyl pyrrolidone (PVP) K90 dissolving microneedles (DMNs). Various printing parameters, such as curing time, printing angle, and anti-aliasing (AA), were varied to evaluate suitable printing conditions for each shape. Furthermore, physical appearance, mechanical property, and skin insertion ability of HPMC/PVP K90 DMNs were examined. The results showed that for shape A and C, the suitable curing time and printing angle were 1.5 s and 30° while for shapes B and D, they were 2.0 s and 45°, respectively. All four shapes required AA to eliminate their stair-stepped edges. Additionally, it was demonstrated that all twelve designs of 3D-printed MN patches could be employed for fabricating MN molds. HPMC/PVP K90 DMNs with the needles of shape A and B exhibited better physicochemical properties compared to those of shape C and D. Particularly, both sample 9 and 10 displayed sharp needle without bent tips, coupled with minimal height reduction (
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- 2024
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3. Carboxymethyl Chitosan-Based Materials in Packaging, Food, Pharmaceutical, and Cosmetics
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Rachtanapun, P., Rachtanapun, C., Jantrawut, P., Thanakkasaranee, S., Kasi, G., Tantala, J., Panraksa, P., Chaiwarit, T., and Jayakumar, R., editor
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- 2024
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4. Itaconic Acid Cross-Linked Biomolecule Immobilization Approach on Amine-Functionalized Silica Nanoparticles for Highly Sensitive Enzyme-Linked Immunosorbent Assay (ELISA)
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Suruk Udomsom, Kritsana Kanthasap, Pathinan Paengnakorn, Pensak Jantrawut, Sarawut Kumphune, Sansanee Auephanwiriyakul, Ukrit Mankong, Nipon Theera-Umpon, and Phornsawat Baipaywad
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Chemistry ,QD1-999 - Published
- 2024
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5. Preparation and stability investigation of ultrasound-assisted W/O/W multiple nanoemulsions co-loaded with hydrophobic curcumin and hydrophilic arbutin for tyrosinase inhibition
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Mathukorn Sainakham, Bouachompoo Promma, Arthima Ngernthong, Kanokwan Kiattisin, Korawinwich Boonpisuttinant, Krisada Wuttikul, Pensak Jantrawut, and Warintorn Ruksiriwanich
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Nanoemulsion ,Tyrosinase inhibition ,Anti-melanogenesis ,Cytotoxicity ,Stability test ,Science (General) ,Q1-390 ,Social sciences (General) ,H1-99 - Abstract
In the present, whitening products are most popular in the cosmetics market, and nanoemulsions are effective drug delivery systems through the skin. The objective of this study was to investigate multiple nanoemulsion formulations for lightning skin effects. The method of this study was the selection of active compounds based on synergistic tyrosinase inhibition activity, formulation preparation by low and high energy methods, physicochemical property determination, stability test, cell toxicity, and anti-melanogenesis in cell culture. From the results, it was found that tyrosinase inhibition with substrate l-tyrosine from the mixture of curcumin and alpha-arbutin gave the highest activity with an IC50 of 63.58 ± 4.99 μM, showed a synergistic effect at a CI value of 0.99, and selected these compounds to develop formulations by the low energy method. However, the most formulations prepared by this method were unstable and phase separated, while the high energy method gave the most formulations with good properties, which were selected for further investigation. The best formulation was 2DS which showed internal droplet morphology in the range of nanometers under a TEM microscope. For 3 months stability test, the formulations had no phase separation and gave the slightly changed values of particle size, polydispersity index (PDI), zeta potentials, and pH values. In addition, multiple nanoemulsions also enhanced the stability of active compounds, with the highest percentage of remaining content of curcumin and arbutin at 94.69 and 90.45 %, respectively at 4 °C for 3 months. In a cell culture test on B16F10, 2DS at 0.05 g/ml gave no cell cytotoxicity and anti-melanogenesis at 57.75 ± 5.74 %, the same potency as kojic acid at a concentration of 20 μg/ml. Therefore, this study will be useful to prepare multiple nanoemulsions for further development into novel health care products.
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- 2024
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6. Synergistic Phytochemical and Pharmacological Actions of Hair RiseTM Microemulsion: A Novel Herbal Formulation for Androgenetic Alopecia and Hair Growth Stimulation
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Anurak Muangsanguan, Warintorn Ruksiriwanich, Pichchapa Linsaenkart, Pensak Jantrawut, Pornchai Rachtanapun, Kittisak Jantanasakulwong, Sarana Rose Sommano, Korawan Sringarm, Chaiwat Arjin, Mathukorn Sainakham, and Juan M. Castagnini
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rice bran (Oryza sativa L.) ,shallot bulb (Allium ascalonicum L.) ,licorice root (Glycyrrhiza glabra L.) ,corn kernels (Zea mays L.) ,androgenetic alopecia ,Sonic Hedgehog ,Botany ,QK1-989 - Abstract
Androgenetic alopecia (AGA) is a genetic condition characterized by an excessive response to androgens, leading to hairline regression in men and hair thinning at the vertex in women, which can negatively impact self-esteem. Conventional synthetic treatments for AGA are often limited by their side effects. In contrast, Thai medicinal plants offer a promising alternative with fewer adverse effects. This study investigates the synergistic phytochemical and pharmacological effects of a novel Hair RiseTM microemulsion, formulated with bioactive extracts from rice bran (Oryza sativa), shallot bulb (Allium ascalonicum), licorice root (Glycyrrhiza glabra), and corn kernels (Zea mays), for the treatment of hair loss. The microemulsion, in concentrations of 50%, 75%, and 100% (v/v), significantly enhanced the proliferation of human hair follicle dermal papilla cells (HFDPCs) compared to minoxidil. Additionally, it upregulated critical hair growth signaling pathways, including Wnt/β-catenin (CTNNB1), Sonic Hedgehog (SHH, SMO, GLI1), and vascular endothelial growth factor (VEGF), surpassing standard controls such as minoxidil and purmorphamine. The microemulsion also demonstrated potent anti-inflammatory and antioxidant properties by reducing nitric oxide production and oxidative stress, factors that contribute to inflammation and follicular damage in AGA. Furthermore, Hair RiseTM inhibited 5α-reductase (types 1–3), a key enzyme involved in androgen metabolism, in both human prostate cancer cells (DU-145) and HFDPCs. These findings suggest that Hair RiseTM microemulsion presents a promising natural therapy for promoting hair growth and reducing hair loss via multiple synergistic mechanisms, offering a potent, plant-based alternative to synthetic treatments.
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- 2024
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7. Design and Optimization of 3D-Printed Tablets Containing Mucuna Extracts for Erectile Dysfunction Management: A DoE-Guided Study
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Ratchapoom Wattanawiggan, Sunee Chansakaow, Pensak Jantrawut, Pattaraporn Panraksa, Jutamas Jiaranaikulwanitch, Suruk Udomsom, Patnarin Worajittiphon, and Pratchaya Tipduangta
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3D printing ,erectile dysfunction ,DoE ,Mucuna extract ,optimization ,sustained release ,Botany ,QK1-989 - Abstract
Erectile dysfunction (ED) refers to the inability of the penis to maintain a firm erection during sexual activity. Mucuna, or M. pruriens, contains levodopa, a compound showing promise in ED treatment. However, formulating Mucuna extract into tablet dosage forms is challenging due to its semisolid nature. This study aimed to develop sustained-release tablets containing Mucuna extract via semisolid extrusion 3D printing. Eudragit RS PO (Eudragit) served as a sustained-release polymer, with poly (vinyl alcohol) (PVA) as a co-polymer for forming the tablet matrices. This study had the following two main phases: screening, which identified the factors affecting the printability, and optimization, which focused on the factors influencing the levodopa release and its consistency. The results showed that both the polymeric solid percentage content (PSPC) in the semisolid slurry and the Eudragit-PVA ratio significantly affected the printability. All of the formulations were printable, and the PSPC and Eudragit-PVA ratios were incorporated into the optimized model. The desired formulation, achieving targeted levodopa release and consistency, had a PSPC of 58.8% and a Eudragit-PVA ratio of 2.87:1. In conclusion, semisolid extrusion 3D printing guided by the design of experiments (DoE) proved feasible for producing reliable 3D-printed tablets with consistent active ingredients and desired release rates.
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- 2024
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8. Fabrication and Characterization of Dissolving Microneedles Containing Oryza sativa L. Extract Complex for Enhancement of Transfollicular Delivery
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Tanpong Chaiwarit, Baramee Chanabodeechalermrung, Pensak Jantrawut, Warintorn Ruksiriwanich, and Mathukorn Sainakham
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dissolving microneedles ,Oryza sativa L. extract complex ,transfollicular penetration ,Organic chemistry ,QD241-441 - Abstract
Dissolving microneedles are extensively applied in drug delivery systems to enhance penetration into the skin. In this study, dissolving microneedles fabricated from polyvinylpyrrolidone K90 (PVP-K90) and hydroxypropylmethyl cellulose (HPMC) E50 in different ratios were characterized. The selected formulations incorporated Oryza sativa L. extract complex and its characteristics, transfollicular penetration, and safety were observed. The microneedles, fabricated from PVP K90: HPMC E50 in a ratio of 25:5 (P25H5) and 20:10 (P20H10), revealed excellent morphological structure, proper mechanical strength, and excellent skin insertion. P25H5 microneedles exhibited faster dissolution than P20H10 microneedles. Microneedles containing Oryza sativa L. extract complex showed excellent morphological structure via scanning electron microscopy but decreased mechanical strength. P25H5-O, which exhibited an effective ability to enter skin, was selected for further investigation. This microneedle formulation had a high percentage of drug-loading content, enhanced skin penetration via the transfollicular route, and was safe for keratinocytes. As a result, the dissolving microneedle containing Oryza sativa L. extract complex can be used to enhance transfollicular delivery through the skin with safety.
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- 2024
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9. Preparation and characterization of ultrasound-assisted nanoemulsions containing natural oil for anti-aging effect
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Kanokwan Kiattisin, Panithan Srithongchai, Warunchana Jaiyong, Korawinwich Boonpisuttinant, Warintorn Ruksiriwanich, Pensak Jantrawut, and Mathukorn Sainakham
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Nanoemulsion ,Natural oil ,Antioxidant activity ,Collagenase inhibition ,Agriculture (General) ,S1-972 ,Nutrition. Foods and food supply ,TX341-641 - Abstract
This research investigated the properties including appearance, rheology, peroxide value and bioactivities of three types of natural oils. Ultrasound-assisted nanoemulsions of the selected natural oils were prepared, evaluated for physical appearance, droplet size, polydispersity index, zeta potential using Zetasizer®, turbidity change measured by UV-VIS spectrophotometer and observed after stability test. After that, nanoemulsions with a particle size less than 200 nm will be selected and evaluated for cytotoxicity and collagen biosynthesis on human skin fibroblasts. The result showed that avocado oil gave the lowest peroxide value and IC50 value by ABTS assay at 6.65 and 30.50 ± 0.98 μg/ml, respectively. Finally, avocado oil, which showed moderate anti-aging activity, was chosen to develop nanoemulsions by an ultrasonic processor. The best nanoemulsion formulation, PS21, contained 8 % avocado oil, 7.5 % PEG 40 hydrogenated castor oil and 2.5 % Span 80 with a small droplet size, narrow polydispersity index and high zeta potential at 68.30 ± 3.08 nm, 0.24 ± 0.08, and −55.10 ± 2.66 mV, respectively. After the stability test, PS21 presented a good physical appearance with no phase separation, an acceptable range of droplet size, a narrow size distribution and slightly changed zeta potentials and turbidity. In the cell culture experiment, cell viability was greater than 80 % for all selected nanoemulsions at a concentration of 0.1 % v/v in comparison to the control group. However, collagen stimulation ranging from 2.27 ± 0.25 to 16.67 ± 2.30 % was observed in only four nanoemulsions, or 50 % of the total sample group. PS21 at a concentration of 0.1 % v/v gave the highest collagen stimulation with no cytotoxicity in human skin fibroblasts. According to all results, avocado oil can be formulated into nanoemulsions with good appearance and stability that are suitable for the further development of novel foods and health care products.
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- 2024
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10. Modification of a Carboxymethyl Cellulose/Poly(vinyl alcohol) Hydrogel Film with Citric Acid and Glutaraldehyde Crosslink Agents to Enhance the Anti-Inflammatory Effectiveness of Triamcinolone Acetonide in Wound Healing
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Kanticha Pratinthong, Winita Punyodom, Pensak Jantrawut, Kittisak Jantanasakulwong, Wirongrong Tongdeesoontorn, Montira Sriyai, Rangsan Panyathip, Sarinthip Thanakkasaranee, Patnarin Worajittiphon, Nuttapol Tanadchangsaeng, and Pornchai Rachtanapun
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hydrogel ,carboxymethyl cellulose ,poly (vinyl alcohol) ,crosslinking ,triamcinolone acetonide ,Organic chemistry ,QD241-441 - Abstract
Anti-inflammatory wound healing involves targeted drug delivery to the wound site using hydrogel materials to prolong drug effectiveness. In this work, hydrogel films were fabricated using carboxymethyl cellulose (CMC) and poly(vinyl alcohol) (PVA) crosslinked with citric acid (CA) and glutaraldehyde (GA) at different concentrations. The crosslinker densities were optimized with various CA (2–10% w/v) and GA (1–5% v/v) concentrations. The optimized crosslink densities in the hydrogel exhibited additional functional group peaks in the FT-IR spectra at 1740 cm−1 for the C=O stretching of the ester linkage in CA and at 1060 cm−1 for the C-O-C stretching of the ether group in GA. Significantly, the internal porous structures of hydrogel composite films improved density, swelling capacities, solubility percentage reduction, and decreased water retention capacities with optimized crosslinker densities. Therefore, these hydrogel composite films were utilized as drug carriers for controlled drug release within 24 h during medical treatment. Moreover, the hydrogel films demonstrated increased triamcinolone acetonide (TAA) absorption with higher crosslinker density, resulting in delayed drug release and improved TAA efficiency in anti-inflammatory activity. As a result, the modified hydrogel showed the capability of being an alternative material with enhanced anti-inflammatory efficiency with hydrogel films.
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- 2024
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11. Potential of green extraction using edible deep eutectic solvents on the bioactivities from Curcuma aromatica rhizome extracts for food application
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Mathukorn Sainakham, Pensak Jantrawut, Kanokwan Kiattisin, Chuda Chittasupho, and Sudarshan Singh
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Curcuma aromatica ,Deep eutectic solvents ,Antioxidant activity ,Monophenolase ,Anti-inflammatory ,Macrophages ,Agriculture (General) ,S1-972 ,Nutrition. Foods and food supply ,TX341-641 - Abstract
Curcuma aromatica (CA) rhizomes are abundant in medicinal phytochemicals and have been reported to promote a variety of health conditions. Deep eutectic solvents (DESs) are an alternative system of liquid at room temperature with advantageous features. The purpose of this study was to evaluate the extraction process, preliminary phytochemical content, bioactivities, anti-inflammatory activity on RAW 264.7 cells and HPLC analysis of edible DESs extracts of CA rhizomes. DESs extracts had extraction yields of total phenolic content ranging from 2.67 ± 0.17 to 19.36 ± 0.26 mg GAE/g extract and total flavonoid content ranging from 30.27 ± 1.36 to 259.19 ± 1.13 mg QE/g extract. The IC50 values of free radical scavenging by DPPH assay, lipid peroxidation inhibition and tyrosinase inhibition of DESs extracts were 20.60 ± 5.50, 11.02 ± 3.56 and 11.18 ± 0.02 mg/ml, respectively. The DESs extracts exhibited nitric oxide radical scavenging ranging from 3.62 ± 1.94 to 48.86 ± 4.30 % and hyaluronidase inhibition ranging from 29.41 ± 0.41 to 40.13 ± 0.56 %. The extracts containing lactic acid and glucose (D2), citric acid and fructose (D5) and citric acid and glucose (D6) exhibited no cytotoxic effects at concentrations ranging from 0.03 to 3.91 μg/ml. The IC50 values of these extracts on anti-inflammatory activity were 0.64 ± 0.01 to 1.25 ± 0.35 μg/ml. In HPLC analysis, D2, D5 and D6 had the content of curcumin per extract at 74.05 ± 0.86, 13.76 ± 0.46 and 31.08 ± 0.72 μg/g, respectively. This study demonstrated the potential application of green extraction using edible DESs on medicinal plants for the further development of novel health care products.
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- 2023
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12. HPMC/PVP K90 Dissolving Microneedles Fabricated from 3D-Printed Master Molds: Impact on Microneedle Morphology, Mechanical Strength, and Topical Dissolving Property
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Baramee Chanabodeechalermrung, Tanpong Chaiwarit, Siripat Chaichit, Suruk Udomsom, Phornsawat Baipaywad, Patnarin Worajittiphon, and Pensak Jantrawut
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dissolving microneedles ,3D printing ,lidocaine HCl ,intradermal drug delivery ,hydroxypropyl methylcellulose ,PVP K90 ,Organic chemistry ,QD241-441 - Abstract
Three-dimensional (3D) printing can be used to fabricate custom microneedle (MN) patches instead of the conventional method. In this work, 3D-printed MN patches were utilized to fabricate a MN mold, and the mold was used to prepare dissolving MNs for topical lidocaine HCl (L) delivery through the skin. Topical creams usually take 1–2 h to induce an anesthetic effect, so the delivery of lidocaine HCl from dissolving MNs can allow for a therapeutic effect to be reached faster than with a topical cream. The dissolving-MN-patch-incorporated lidocaine HCl was constructed from hydroxypropyl methylcellulose (HPMC; H) and polyvinyl pyrrolidone (PVP K90; P) using centrifugation. Additionally, the morphology, mechanical property, skin insertion, dissolving behavior, drug-loading content, drug release of MNs and the chemical interactions among the compositions were also examined. H51P2-L, H501P2-L, and H901P2-L showed an acceptable needle appearance without bent tips or a broken structure, and they had a low % height change (80%). These three formulations exhibited a drug-loading content approaching 100%. Importantly, the composition-dependent dissolving abilities of MNs were revealed. Containing the lowest amount of HPMC in its formulation, H901P2-L showed the fastest dissolving ability, which was related to the high amount of lidocaine HCl released through the skin. Moreover, the results of an FTIR analysis showed no chemical interactions among the two polymers and lidocaine HCl. As a result, HPMC/PVP K90 dissolving microneedles can be used to deliver lidocaine HCl through the skin, resulting in a faster onset of anesthetic action.
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- 2024
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13. Botanical Biometrics: Exploring Morphological, Palynological, and DNA Barcoding Variations in White Kwao Krua (Pueraria candollei Grah. ex Benth. and P. mirifica Airy Shaw & Suvat.)
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Wannaree Charoensup, Aekkhaluck Intharuksa, Suthira Yanaso, Suthiwat Khamnuan, Sunee Chansakaow, Panee Sirisa-ard, Pensak Jantrawut, Charan Ditchaiwong, and Kesorn Chaemcheun
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DNA barcoding ,herbal medicine ,matK ,morphological characteristics ,palynological characters ,phylogentic analysis ,Plant culture ,SB1-1110 - Abstract
White Kwao Krua, a crucial Thai medicinal plant, contains various phytoestrogen substances used to alleviate menopausal symptoms in estrogen-deficient women. It originates from two species, namely Pueraria candollei Wall. ex Benth. and P. mirifica Airy Shaw & Suvat. However, there exists morphological variation, and the taxonomic status between both species is ambiguous, making discrimination challenging. In this study, we aim to clarify and differentiate the morphological characteristics, palynology, and DNA barcoding of both species. The morphological results showed the stipule size is a phenotypic marker for the differentiation of both species during the vegetative stage. The palynological results, however, exhibited similarity. Through an examination of nucleotide sequences and neighbor-joining tree analysis, it was determined that the DNA barcoding of the matK region has the capability to distinguish between P. candollei and P. mirifica at nucleotide position 702. Specifically, P. candollei manifested a G base, contrasting with the C base observed in P. mirifica. This study concludes that stipule size and the matK gene in DNA barcoding serve as a distinctive characteristic for distinguishing between P. candollei and P. mirifica. These methodologies prove valuable for ensuring the accurate identification of white Kwao Krua for horticulturists.
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- 2024
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14. Carboxymethyl Chitosan-Based Materials in Packaging, Food, Pharmaceutical, and Cosmetics
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Rachtanapun, P., primary, Rachtanapun, C., additional, Jantrawut, P., additional, Thanakkasaranee, S., additional, Kasi, G., additional, Tantala, J., additional, Panraksa, P., additional, and Chaiwarit, T., additional
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- 2023
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15. Bamboo Pulp Toughening Poly (Lactic Acid) Composite Using Reactive Epoxy Resin
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Krittameth Kiattipornpithak, Pornchai Rachtanapun, Sarinthip Thanakkasaranee, Pensak Jantrawut, Warintorn Ruksiriwanich, Sarana Rose Sommano, Noppol Leksawasdi, Thorsak Kittikorn, and Kittisak Jantanasakulwong
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poly (lactic acid) ,bamboo pulp ,epoxy resin ,reaction ,reactive melt blending ,reactive into cross-linking reaction ,Organic chemistry ,QD241-441 - Abstract
A novel poly (lactic acid) (PLA) composite with excellent mechanical properties, toughness, thermal stability, and water resistance was developed using a reactive melt-blending technique. PLA was melt mixed with epoxy resin (EPOXY) and bamboo pulp (PULP) to improve its reaction and mechanical properties. FTIR analysis confirmed the successful reaction of the PLA/EPOXY/PULP composites; the epoxy groups of EPOXY reacted with the –COOH groups of PLA and the –OH groups of PULP. The PLA/EPOXY/PULP5 composite showed a high tensile strength (67 MPa) and high toughness of 762 folding cycles, whereas the highest tensile strength was 77 MPa in the PLA/EPOXY5/PULP20 sample. SEM images presented a gap between the PLA and PULP; gap size decreased with the addition of EPOXY. The Tg of the PLA decreased with the EPOXY plasticizer effect, whereas the Tm did not significantly change. PULP induced crystallinity and increased Vicat softening of the PLA/PULP and PLA/EPOXY/PULP composites. The EPOXY reaction of the PLA/PULP composites improved their tensile properties, toughness, thermal stability, and water resistance.
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- 2023
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16. Surface-Modified Carboxylated Cellulose Nanofiber Hydrogels for Prolonged Release of Polyhexamethylene Biguanide Hydrochloride (PHMB) for Antimicrobial Applications
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Pichapar O-chongpian, Tanpong Chaiwarit, Kittisak Jantanasakulwong, Pornchai Rachtanapun, Patnarin Worajittiphon, Nutthapong Kantrong, and Pensak Jantrawut
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carboxylated cellulose nanofibers ,hydrogel ,TEMPO oxidization ,polyhexamethylene biguanide hydrochloride ,antimicrobial applications ,Organic chemistry ,QD241-441 - Abstract
The surface modification of cellulose nanofibers (CNFs) using a 2,2,6,6-tetramethylpiperidine-1-oxyl (TEMPO)/sodium bromide (NaBr)/sodium hypochlorite (NaClO) system was successful in improving their hydrophilicity. Following that, we fabricated hydrogels containing carboxylated cellulose nanofibers (c-CNFs) and loaded them with polyhexamethylene biguanide (PHMB) using a physical crosslinking method, aiming for efficient antimicrobial uses. The morphological and physicochemical properties of all hydrogel formulations were characterized, and the results revealed that the 7% c-CNFs-2 h loaded with PHMB formulation exhibited desirable characteristics such as regular shape, high porosity, good mechanical properties, suitable gel content, and a good maximum swelling degree. The successful integration of PHMB into the c-CNF matrix was confirmed by FTIR analysis. Furthermore, the 7% c-CNFs-2 h loaded with the PHMB formulation demonstrated PHMB contents exceeding 80% and exhibited a prolonged drug release pattern for up to 3 days. Moreover, this formulation displayed antibacterial activity against S. aureus and P. aeruginosa. In conclusion, the novel approach of c-CNF hydrogels loaded with PHMB through physical crosslinking shows promise as a potential system for prolonged drug release in topical drug delivery while also exhibiting excellent antibacterial activity.
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- 2023
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17. Reactive Blending of Modified Thermoplastic Starch Chlorhexidine Gluconate and Poly(butylene succinate) Blending with Epoxy Compatibilizer
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Nanthicha Thajai, Pornchai Rachtanapun, Sarinthip Thanakkasaranee, Winita Punyodom, Patnarin Worajittiphon, Yuthana Phimolsiripol, Noppol Leksawasdi, Sukunya Ross, Pensak Jantrawut, and Kittisak Jantanasakulwong
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biopolymer ,modified starch ,chlorhexidine gluconate ,poly (butylene succinate) ,epoxy resin ,Organic chemistry ,QD241-441 - Abstract
Biodegradable starch-based polymers were developed by melt-blending modified thermoplastic starch (MTPS) with poly(butylene succinate) (PBS) blended with epoxy resin (Er). A modified thermoplastic starch blend with chlorhexidine gluconate (MTPSCh) was prepared by melt-blending cassava starch with glycerol and chlorhexidine gluconate (CHG) 1.0% wt. The Er was melt-blended with PBS (PBSE) at concentrations of 0.50%, 1.0%, 2.5%, and 5.0% (wt%/wt%). The mechanical properties, water resistance, and morphology of the MTPSCh/PBSE blends were investigated. The MTPSCh/PBSE2.5% blend showed an improvement in tensile strength (8.1 MPa) and elongation at break (86%) compared to the TPSCh/PBS blend (2.6 MPa and 53%, respectively). In addition, water contact angle measurements indicated an increase in the hydrophobicity of the MTPSCh/PBSE blends. Thermogravimetric analysis showed an improvement in thermal stability when PBS was added to the MTPSCh blends. Fourier transform infrared spectroscopy data confirmed a new reaction between the amino groups of CHG in MTPSCh and the epoxy groups of Er in PBSE, which improved the interfacial adhesion of the MTPSCh/PBSE blends. This reaction improved the mechanical properties, water resistance, morphology, and thermal stability of the TPSCh/PBSE blends.
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- 2023
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18. Sericin cocoon bio-compatibilizer for reactive blending of thermoplastic cassava starch
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Thanongsak Chaiyaso, Pornchai Rachtanapun, Nanthicha Thajai, Krittameth Kiattipornpithak, Pensak Jantrawut, Warintorn Ruksiriwanich, Phisit Seesuriyachan, Noppol Leksawasdi, Yuthana Phimolsiripol, Charin Techapun, Sarana Rose Sommano, Toshiaki Ougizawa, Kamon Yakul, and Kittisak Jantanasakulwong
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Medicine ,Science - Abstract
Abstract Cassava starch was blended with glycerol to prepare thermoplastic starch (TPS). Thermoplastic starch was premixed with sericin (TPSS) by solution mixing and then melt-blended with polyethylene grafted maleic anhydride (PEMAH). The effect of sericin on the mechanical properties, morphology, thermal properties, rheology, and reaction mechanism was investigated. The tensile strength and elongation at break of the TPSS10/PEMAH blend were improved to 12.2 MPa and 100.4%, respectively. The TPS/PEMAH morphology presented polyethylene grafted maleic anhydride particles (2 μm) dispersed in the thermoplastic starch matrix, which decreased in size to approximately 200 nm when 5% sericin was used. The melting temperature of polyethylene grafted maleic anhydride (121 °C) decreased to 111 °C because of the small crystal size of the polyethylene grafted maleic anhydride phase. The viscosity of TPS/PEMAH increased with increasing sericin content because of the chain extension. Fourier-transform infrared spectroscopy confirmed the reaction between the amino groups of sericin and the maleic anhydride groups of polyethylene grafted maleic anhydride. This reaction reduced the interfacial tension between thermoplastic starch and polyethylene grafted maleic anhydride, which improved the compatibility, mechanical properties, and morphology of the blend.
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- 2021
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19. Corn starch reactive blending with latex from natural rubber using Na+ ions augmented carboxymethyl cellulose as a crosslinking agent
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Noppol Leksawasdi, Thanongsak Chaiyaso, Pornchai Rachtanapun, Sarinthip Thanakkasaranee, Pensak Jantrawut, Warintorn Ruksiriwanich, Phisit Seesuriyachan, Yuthana Phimolsiripol, Charin Techapun, Sarana Rose Sommano, Toshiaki Ougizawa, and Kittisak Jantanasakulwong
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Medicine ,Science - Abstract
Abstract A mixture of corn starch and glycerol plasticizer (CSG) was blended with latex natural rubber (LNR) and carboxymethyl cellulose (CMC). The addition of 10 phr of CMC improved the Young’s modulus (6.7 MPa), tensile strength (8 MPa), and elongation at break (80%) of the CSG/LNR blend. The morphology of the CSG/LNR/CMC blends showed a uniform distribution of LNR particles (1–3 µm) in the CSG matrix. The addition of CMC enhanced the swelling ability and water droplet contact angle of the blends owing to the swelling properties, interfacial crosslinking, and amphiphilic structure of CMC. Fourier transform infrared spectroscopy confirmed the reaction between the C=C bond of LNR and the carboxyl groups (–COO−) of CMC, in which the Na+ ions in CMC acted as a catalyst. Notably, the mechanical properties of the CSG/LNR/CMC blend were improved owing to the miscibility of CSG/CMC and the CMC/LNR interfacial reaction. The CSG/LNR/CMC biodegradable polymer with high mechanical properties and interfacial tension can be used for packaging, agriculture, and medical applications.
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- 2021
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20. Wound Healing Effect of Supercritical Carbon Dioxide Datura metel L. Leaves Extracts: An In Vitro Study of Anti-Inflammation, Cell Migration, MMP-2 Inhibition, and the Modulation of the Sonic Hedgehog Pathway in Human Fibroblasts
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Warintorn Ruksiriwanich, Pichchapa Linsaenkart, Anurak Muangsanguan, Korawan Sringarm, Pensak Jantrawut, Chaiwat Arjin, Sarana Rose Sommano, Yuthana Phimolsiripol, and Francisco J. Barba
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Datura metel ,cell migration ,sonic hedgehog pathway ,stimulation of collagen synthesis ,supercritical carbon dioxide extraction ,wound healing ,Botany ,QK1-989 - Abstract
Datura metel L. (thorn apple) has been used in Thai folk wisdom for wound care. In this study, we chose supercritical carbon dioxide extraction (scCO2) to develop crude extraction from the leaves of the thorn apple. The phytochemical profiles were observed using liquid chromatography–quadrupole time-of-flight mass spectrometry (LC-QTOF-MS). The biological activities of D. metel were performed through antioxidant assays, anti-inflammation based on the Griess reaction, the migration assay, the expression of matrix metalloproteinase-2 (MMP-2), and regulatory genes in fibroblasts. Dm1 and Dm2 extracts were obtained from scCO2 procedures at different pressures of 300 and 500 bar, respectively. Bioactive compounds, including farnesyl acetone, schisanhenol B, and loliolide, were identified in both extracts. The antioxidant properties of both D. metel extracts were comparable to those of l-ascorbic acid in hydrogen peroxide-induced fibroblasts with no significant difference. Additionally, Dm1 and Dm2 significantly inhibited the nitrite production levels of 1.23 ± 0.19 and 1.52 ± 0.05 μM, respectively, against the lipopolysaccharide-treated group (3.82 ± 0.39 μM). Interestingly, Dm1 obviously demonstrated the percentage of wound closure with 58.46 ± 7.61 and 82.62 ± 6.66% after 36 and 48 h of treatment, which were comparable to the commercial deproteinized dialysate from the calf blood extract. Moreover, both extracts were comparable to l-ascorbic acid treatment in their ability to suppress the expression of MMP-2: an enzyme that breaks down collagen. The gene expressions of SHH, SMO, and GLI1 that control the sonic hedgehog pathway were also clearly upregulated by Dm1. Consequently, the scCO2 technique could be applied in D. metel extraction and contribute to potentially effective wound closure.
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- 2023
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21. Relationship between Phenotypes and Chemical Profiling of Boesenbergia rotunda Collected from Different Habitats of Northern Thailand
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Sureerat Thomya, Malaiporn Wongkaew, Weenun Bundithya, Chompunut Lumsangkul, Pensak Jantrawut, and Sarana Rose Sommano
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antioxidant ,chemical composition ,panduratin A ,pinostrobin ,Zingiberaceae ,Agriculture - Abstract
Fingerroot [Boesenbergia rotunda (Linn.) Mansf] is known to consist of compounds such as panduratin A and pinostrobin with inhibitory property against SARS-CoV-2 infection at both the pre-entry and post-infection phases. Consequently, demand for good phenotype selection has increased in the post-pandemic era. However, the availability of important active ingredients may be influenced by genetic variables and agronomic aspects. Thus, the purpose of this study was to investigate the relationship between characteristics and genotype, and to preliminarily compare the phytochemical profiles of the landraces collected in Northern Thailand. Five local landraces and two wild types were gathered for ex situ collection for morphological assessments, genetic evaluation, and bioactive ingredients (mainly antioxidative potentials and amounts of the active flavonoids). The morphological data were able to distinguish the plant samples to those of wild, cultivated, and adaptive types, which was confirmed by their distinctive genetic variations. However, there was no correlation between the physical attributes and the amount of their bioactive constituents. It was also observed that the adaptation of plants to environmental conditions had a pronounced impact on secondary metabolite biosynthesis, and that such adaptations were likely influenced by genetic differentiation. The findings from this study could potentially be used to improve the cultivation, selection, and breeding of this plant species for desired traits such as increased bioactive compound content, or for conservation and restoration efforts of the landraces and wild types.
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- 2023
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22. Natural Melanogenesis Inhibitor, Antioxidant, and Collagen Biosynthesis Stimulator of Phytochemicals in Rice Bran and Husk Extracts from Purple Glutinous Rice (Oryza sativa L. cv. Pieisu 1 CMU) for Cosmetic Application
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Pichchapa Linsaenkart, Warintorn Ruksiriwanich, Pensak Jantrawut, Chuda Chittasupho, Pornchai Rachtanapun, Kittisak Jantanasakulwong, Sarana Rose Sommano, Chanakan Prom-u-thai, Sansanee Jamjod, Chaiwat Arjin, Korawan Sringarm, and Francisco J. Barba
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anthocyanin ,antioxidant ,collagen stimulator ,matrix metalloproteinase 2 inhibition ,melanogenesis inhibitor ,Oryza sativa ,Botany ,QK1-989 - Abstract
Oryza sativa L. cv. Pieisu 1 CMU (PES1CMU) has a high anthocyanin content in the colored bran and high phenolic content in the husk. Biologically active compounds in plants are available as dietary supplements and cosmetics. To expand the utilization of natural resources, PES1CMU will be a natural remedy for skin hyperpigmentation and aging. Cell-free tyrosinase inhibition and scavenging assays were used to screen all extracts, including PES1CMU-rice bran oil (RBO), PES1CMU-defatted rice bran (DFRB), and PES1CMU-husk (H). PES1CMU extracts were first examined in IBMX-stimulated B16 cells and H2O2-induced fibroblasts. The results exhibited that PES1CMU-DFRB was the most effective inhibitor of mushroom tyrosinase, intracellular melanin production (fold change of 1.11 ± 0.01), and tyrosinase activity (fold change of 1.22 ± 0.10) in IBMX-stimulated B16 cells. Particularly, PES1CMU-DFRB showed a comparable whitening effect to the standard arbutin with no significant difference (p > 0.05). Moreover, PES1CMU-DFRB and PES1CMU-H demonstrated strong scavenging activities. After accelerated cell aging caused by H2O2 exposure in fibroblasts, the levels of malondialdehyde production in all PES1CMU-treated fibroblasts were comparable with those of standard l-ascorbic acid (p > 0.05). Besides, PES1CMU-DFRB and PES1CMU-H treatment significantly inhibited collagen degradation against MMP-2 compared to l-ascorbic acid-treated cells (p > 0.05). PES1CMU rice-processing wastes (DFRB and H) could become potential natural sources for dermatocosmetic constituents in skin anti-aging and whitening products.
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- 2023
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23. Regulatory Effects of Thai Rice By-Product Extracts from Oryza sativa L. cv. Bue Bang 3 CMU and Bue Bang 4 CMU on Melanin Production, Nitric Oxide Secretion, and Steroid 5α-Reductase Inhibition
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Warintorn Ruksiriwanich, Pichchapa Linsaenkart, Chiranan Khantham, Anurak Muangsanguan, Korawan Sringarm, Pensak Jantrawut, Chanakan Prom-u-thai, Sansanee Jamjod, Supapohn Yamuangmorn, Chaiwat Arjin, Pornchai Rachtanapun, Kittisak Jantanasakulwong, Yuthana Phimolsiripol, Francisco J. Barba, Sarana Rose Sommano, Romchat Chutoprapat, and Korawinwich Boonpisuttinant
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androgenetic alopecia ,Bue Bang 3 CMU ,Bue Bang 4 CMU ,rice by-products ,hair graying ,hair growth promotion ,Botany ,QK1-989 - Abstract
Alopecia and gray hair are common hair abnormalities affecting physical appearance and causing psychological problems. Chemical treatments partially restore hair disorders but have distressing side effects. Bioactive plant compounds constitute promising sources of potential medicinal substances instead of chemical agents, producing high side effects. In this study, we focused on the waste of local rice cultivars: Bue Bang 3 CMU (BB3CMU) and Bue Bang 4 CMU (BB4CMU) from the north of Thailand. The rice bran oil (RBO), defatted rice bran extract (DFRB), and rice husk (H) were determined for in vitro hair revitalization in melanin production, nitric oxide (NO) secretion, and steroid 5α-reductase inhibition. The results indicated that BB4CMU-RBO with high contents of iron, zinc, and free fatty acids showed a comparable induction of melanin production on melanocytes (130.18 ± 9.13% of control) to the standard drug theophylline with no significant difference (p > 0.05). This promising melanin induction could be related to activating the NO secretion pathway, with the NO secretion level at 1.43 ± 0.05 µM. In addition, BB4CMU-RBO illustrated a significant inhibitory effect on both steroid 5α-reductase genes (SRD5A) type 1 and type 2, which relates to its primary source of tocopherols. Hence, rice bran oil from the Thai rice variety BB4CMU could be applied as a promising hair revitalizing candidate, from natural resources, to help promote hair growth and re-pigmentation effects.
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- 2023
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24. Mango Pectic Oligosaccharides: A Novel Prebiotic for Functional Food
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Malaiporn Wongkaew, Pipat Tangjaidee, Noppol Leksawasdi, Kittisak Jantanasakulwong, Pornchai Rachtanapun, Phisit Seesuriyachan, Yuthana Phimolsiripol, Thanongsak Chaiyaso, Warintorn Ruksiriwanich, Pensak Jantrawut, and Sarana Rose Sommano
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fruit biomass ,intestinal microflora ,lactic acid bacteria ,short-chain fatty acids ,probiotic ,Nutrition. Foods and food supply ,TX341-641 - Abstract
Prebiotics are functional food ingredients that assist probiotic growth and render many other health benefits. Mango peel is the biomass of the processing industry and has recently been value-added as a dietary fiber pectin. Besides its general use as a food additive, mango peel pectin (MPP) is partially hydrolyzed by pectinase to obtain pectic oligosaccharides (POSs) that have recently gained attention as novel prebiotic products and in medical research. This review describes probiotic candidates responsible for the digestion of pectin derivatives and the advantages of POSs as functional additives and their current best retrieval options. Mango pectic oligosaccharide (MPOS) recovery from low methoxyl MPP from mango with prebiotic performance both in vivo and in vitro environments is discussed. Current research gaps and potential developments in the field are also explored. The overall worthiness of this article is the potential use of the cheap-green food processing bioresource for high-value components.
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- 2022
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25. Effects of storage temperature on the quality of eggs coated by cassava starch blended with carboxymethyl cellulose and paraffin wax
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Pornchai Rachtanapun, Nattagarn Homsaard, Araya Kodsangma, Suphat Phongthai, Noppol Leksawasdi, Yuthana Phimolsiripol, Phisit Seesuriyachan, Thanongsak Chaiyaso, Suwit Chotinan, Pensak Jantrawut, Warintorn Ruksiriwanich, Sutee Wangtueai, Sarana Rose Sommano, Wirongrong Tongdeesoontorn, Korawan Sringarm, and Kittisak Jantanasakulwong
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carboxymethyl cellulose ,Manihot esculenta ,egg quality ,nutrition ,microbial ,Animal culture ,SF1-1100 - Abstract
ABSTRACT: A blend of cassava starch (CS), carboxymethyl cellulose (CMC), and paraffin was prepared as a coating material to maintain the quality of eggs during 4 wk of storage at different temperatures. The efficacy of the CS/CMC/paraffin (6/1/0.5% w/v) coating was investigated in terms of the Haugh unit (HU), weight loss, pH, and microbial load at the end of storage. The best egg storage temperature was 4°C, which maintained an HU of grade AA in coated and uncoated eggs for 4 wk. Lower weight loss (2.14%) was observed in coated eggs at 4°C storage than at 30°C storage (3.26%). The pH in the albumen of coated and uncoated eggs at 4°C increased from 6.84 to 6.88 and 7.01 to 7.03, respectively, after 4 wk of storage. No microbes were detected in the coated and uncoated eggs at 4°C. The maximum microbial count was 728 ± 35 cfu/mL in uncoated eggs at 30°C storage. Egg coating prevented microbial contamination of eggs stored at 30°C for 4 wk. The freshness of the eggs did not affect the nutrient content. The egg-coating material effectively maintained egg quality, prevented microbial contamination of eggs, and increased the shelf life of eggs at storage temperatures of 25 and 30°C.
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- 2022
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26. Author Correction: Corn starch reactive blending with latex from natural rubber using Na+ ions augmented carboxymethyl cellulose as a crosslinking agent
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Leksawasdi, Noppol, Chaiyaso, Thanongsak, Rachtanapun, Pornchai, Thanakkasaranee, Sarinthip, Jantrawut, Pensak, Ruksiriwanich, Warintorn, Seesuriyachan, Phisit, Phimolsiripol, Yuthana, Techapun, Charin, Sommano, Sarana Rose, Ougizawa, Toshiaki, and Jantanasakulwong, Kittisak
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- 2021
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27. Sericin cocoon bio-compatibilizer for reactive blending of thermoplastic cassava starch
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Chaiyaso, Thanongsak, Rachtanapun, Pornchai, Thajai, Nanthicha, Kiattipornpithak, Krittameth, Jantrawut, Pensak, Ruksiriwanich, Warintorn, Seesuriyachan, Phisit, Leksawasdi, Noppol, Phimolsiripol, Yuthana, Techapun, Charin, Sommano, Sarana Rose, Ougizawa, Toshiaki, Yakul, Kamon, and Jantanasakulwong, Kittisak
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- 2021
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28. Corn starch reactive blending with latex from natural rubber using Na+ ions augmented carboxymethyl cellulose as a crosslinking agent
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Leksawasdi, Noppol, Chaiyaso, Thanongsak, Rachtanapun, Pornchai, Thanakkasaranee, Sarinthip, Jantrawut, Pensak, Ruksiriwanich, Warintorn, Seesuriyachan, Phisit, Phimolsiripol, Yuthana, Techapun, Charin, Sommano, Sarana Rose, Ougizawa, Toshiaki, and Jantanasakulwong, Kittisak
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- 2021
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29. Guava (Psidium guajava L.) Leaf Extract as Bioactive Substances for Anti-Androgen and Antioxidant Activities
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Warintorn Ruksiriwanich, Chiranan Khantham, Anurak Muangsanguan, Yuthana Phimolsiripol, Francisco J. Barba, Korawan Sringarm, Pornchai Rachtanapun, Kittisak Jantanasakulwong, Pensak Jantrawut, Chuda Chittasupho, Romchat Chutoprapat, Korawinwich Boonpisuttinant, and Sarana Rose Sommano
- Subjects
androgenetic alopecia ,anti-hair loss ,dermal papilla ,DU-145 ,guava ,hair follicle ,Botany ,QK1-989 - Abstract
Leaves of guava (Psidium guajava L.) have been used in Thai folk medicine without any supporting evidence as a traditional herbal remedy for hair loss. Androgenetic alopecia (AGA) is chronic hair loss caused by effects of androgens in those with a genetic predisposition, resulting in hair follicle miniaturization. Our objectives were to provide the mechanistic assessment of guava leaf extract on gene expressions related to the androgen pathway in well-known in vitro models, hair follicle dermal papilla cells (HFDPC), and human prostate cancer cells (DU-145), and to determine its bioactive constituents and antioxidant activities. LC-MS analysis demonstrated that the main components of the ethanolic extract of guava leaves are phenolic substances, specifically catechin, gallic acid, and quercetin, which contribute to its scavenging and metal chelating abilities. The guava leaf extract substantially downregulated SRD5A1, SRD5A2, and SRD5A3 genes in the DU-145 model, suggesting that the extract could minimize hair loss by inhibiting the synthesis of a potent androgen (dihydrotestosterone). SRD5A suppression by gallic acid and quercetin was verified. Our study reveals new perspectives on guava leaf extract’s anti-androgen properties. This extract could be developed as alternative products or therapeutic adjuvants for the treatment of AGA and other androgen-related disorders.
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- 2022
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30. Fabrication and Evaluation of Water Hyacinth Cellulose-Composited Hydrogel Containing Quercetin for Topical Antibacterial Applications
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Tanpong Chaiwarit, Baramee Chanabodeechalermrung, Nutthapong Kantrong, Chuda Chittasupho, and Pensak Jantrawut
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water hyacinth ,cellulose ,hydrogel ,quercetin ,antibacterial ,Science ,Chemistry ,QD1-999 ,Inorganic chemistry ,QD146-197 ,General. Including alchemy ,QD1-65 - Abstract
Water hyacinth is an aquatic weed species that grows rapidly. In particular, it causes negative impacts on the aquatic environment and ecological system. However, water hyacinth is rich in cellulose, which is a biodegradable material. This study isolated cellulose from the water hyacinth petiole. It was then used to fabricate composite hydrogels made with water hyacinth cellulose (C), alginate (A), and pectin (P) at different mass ratios. The selected water hyacinth cellulose-based hydrogel was incorporated with quercetin, and its properties were evaluated. The FTIR and XRD of extracted water hyacinth cellulose indicated specific characteristics of cellulose. The hydrogel which consisted of the water hyacinth cellulose alginate characterized pectin: pectin had a mass ratio of 2.5:0.5:0.5 (C2.5A0.5P0.5), showed good puncture strength (2.16 ± 0.14 N/mm2), the highest swelling index (173.28 ± 4.94%), and gel content (39.35 ± 0.53%). The FTIR showed an interaction between water hyacinth cellulose and quercetin with hydrogen bonding. The C2.5A0.5P0.5 hydrogel containing quercetin possessed 92.07 ± 5.77% of quercetin-loaded efficiency. It also exhibited good antibacterial activity against S. aureus and P. aeruginosa due to hydrogel properties, and no toxicity to human cells. This study indicated that water hyacinth cellulose-composited hydrogel is suitable for topical antibacterial applications.
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- 2022
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31. Fabrication of Hydroxypropyl Methylcellulose Orodispersible Film Loaded Mirtazapine Using a Syringe Extrusion 3D Printer
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Tanpong Chaiwarit, Niphattha Aodsab, Pimonnart Promyos, Pattaraporn Panraksa, Suruk Udomsom, and Pensak Jantrawut
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orodispersible film ,3D-printing ,hydroxypropyl methylcellulose ,mirtazapine ,depression ,Pharmacy and materia medica ,RS1-441 - Abstract
Depression is a mental illness causing a continuous negative feeling and loss of interest and affects physical and mental health. Mirtazapine (MTZ) is an effective medicine for treating depression, but patients lack compliance. However, transforming a pharmaceutical dosage form to an orodispersible film (ODF) could resolve this issue. This study aims to fabricate ODF-loading mirtazapine, using a syringe extrusion 3D printer, and compare its properties with the solvent-casting method. The ODFs were prepared by dissolving the mirtazapine in a hydroxypropyl methylcellulose E15 solution, and then fabricated by a 3D printer or casting. The 3D printing was accurate and precise in fabricating the ODFs. The SEM micrographs showed that the mirtazapine-printed ODF (3D-MTZ) was porous, with crystals of mirtazapine on the film’s surface. The 3D-MTZ exhibited better mechanical properties than the mirtazapine-casted ODF (C-MTZ), due to the 3D-printing process. The disintegration time of the 3D-MTZ in a simulated salivary fluid, pH 6.8 at 37 °C, was 24.38 s, which is faster than the C-MTZ (46.75 s). The in vitro dissolution study, in 0.1 N HCl at 37 °C, found the 3D-MTZ quickly released the drug by more than 80% in 5 min. This study manifested that 3D-printing technology can potentially be applied for the fabrication of ODF-containing mirtazapine.
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- 2022
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32. Sparking Nano-Metals on a Surface of Polyethylene Terephthalate and Its Application: Anti-Coronavirus and Anti-Fogging Properties
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Kittisak Jantanasakulwong, Sarinthip Thanakkasaranee, Phisit Seesuriyachan, Pisith Singjai, Aphisit Saenjaiban, Siriphan Photphroet, Kanticha Pratinthong, Yuthana Phimolsiripol, Noppol Leksawasdi, Thanongsak Chaiyaso, Sarana Rose Sommano, Pensak Jantrawut, Siriwadee Chomdej, Suwit Chotinan, Francisco J. Barba, Joe M. Regenstein, Alissara Reungsang, and Pornchai Rachtanapun
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sparking process ,polyethylene terephthalate ,anti-virus ,nanoparticle ,coronavirus ,Biology (General) ,QH301-705.5 ,Chemistry ,QD1-999 - Abstract
The nano-metal-treated PET films with anti-virus and anti-fogging ability were developed using sparking nano-metal particles of Ag, Zn, and Ti wires on polyethylene terephthalate (PET) films. Ag nanoparticles were detected on the PET surface, while a continuous aggregate morphology was observed with Zn and Ti sparking. The color of the Ag-PET films changed to brown with increasing repeat sparking times, but not with the Zn-PET and Ti-PET films. The water contact angle of the nano-metal-treated PET films decreased with increasing repeat sparking times. The RT-PCR anti-virus test confirmed the high anti-virus efficiency of the nano-metal-treated PET films due to the fine particle distribution, high polarity, and binding of the nano-metal ions to the coronavirus, which was destroyed by heat after UV irradiation. A highly transparent, anti-fogging, and anti-virus face shield was prepared using the Zn-PET film. Sparking was an effective technique to prepare the alternative anti-virus and anti-fogging films for medical biomaterial applications because of their low cost, convenience, and fast processing.
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- 2022
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33. Dual Crosslinked Ion-Based Bacterial Cellulose Composite Hydrogel Containing Polyhexamethylene Biguanide
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Baramee Chanabodeechalermrung, Tanpong Chaiwarit, Sarana Rose Sommano, Pornchai Rachtanapun, Nutthapong Kantrong, Chuda Chittasupho, and Pensak Jantrawut
- Subjects
bacterial cellulose ,crosslinking ,hydrogel ,alginate ,pectin ,Chemical technology ,TP1-1185 ,Chemical engineering ,TP155-156 - Abstract
Composite bacterial cellulose (BC) based hydrogel with alginate (A) or pectin (P) or alginate and pectin was fabricated via a physical crosslinking technique using calcium chloride (CaCl2) solution and incorporated with polyhexamethylene biguanide (PHMB) as an effective antimicrobial drug by immersion method. After that, the physicochemical properties of all hydrogel formulations were characterized. The result showed that the formulations with PHMB performed better physicochemical properties than the hydrogel without PHMB. Fourier transform infrared spectroscopy (FT-IR) showed the interaction between PHMB and the carboxylic group of alginate and pectin. BC/A-PHMB hydrogel performed suitable mechanical strength, fluid uptake ability, water retention property, drug content, high integrity value, and maximum swelling degree. Moreover, in vitro cell viability of BC/A-PHMB hydrogel revealed high biocompatibility with human keratinocyte cell line (HaCaT) and demonstrated prolong released of PHMB in Tris-HCl buffer pH 7.4, while rapid release in phosphate buffer saline pH 7.4. BC/A-PHMB hydrogel demonstrated good anti-bacterial activity against S. aureus and P. aeruginosa. In conclusion, BC/A-PHMB hydrogel could be a potential dual crosslinked ion-based hydrogel for wound dressing with anti-bacterial activity.
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- 2022
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34. Itaconic Acid Cross-Linked Biomolecule Immobilization Approach on Amine-Functionalized Silica Nanoparticles for Highly Sensitive Enzyme-Linked Immunosorbent Assay (ELISA).
- Author
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Udomsom, Suruk, Kanthasap, Kritsana, Paengnakorn, Pathinan, Jantrawut, Pensak, Kumphune, Sarawut, Auephanwiriyakul, Sansanee, Mankong, Ukrit, Theera-Umpon, Nipon, and Baipaywad, Phornsawat
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- 2024
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35. Author Correction: Corn starch reactive blending with latex from natural rubber using Na+ ions augmented carboxymethyl cellulose as a crosslinking agent
- Author
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Noppol Leksawasdi, Thanongsak Chaiyaso, Pornchai Rachtanapun, Sarinthip Thanakkasaranee, Pensak Jantrawut, Warintorn Ruksiriwanich, Phisit Seesuriyachan, Yuthana Phimolsiripol, Charin Techapun, Sarana Rose Sommano, Toshiaki Ougizawa, and Kittisak Jantanasakulwong
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Medicine ,Science - Published
- 2021
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36. Formulation and Characterization of Nicotine Microemulsion-Loaded Fast-Dissolving Films for Smoking Cessation
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Kantaporn Kheawfu, Pattaraporn Panraksa, and Pensak Jantrawut
- Subjects
nicotine ,microemulsion ,fast-dissolving film ,buccal film ,Organic chemistry ,QD241-441 - Abstract
The present study aimed to develop a nicotine microemulsion (NCT-ME) and incorporate it into a fast-dissolving film. The NCT-ME was prepared by mixing the specified proportions of nicotine (NCT), surfactant, co-solvent, and water. The NCT-ME was measured by its average droplet size, size distribution, zeta potential, and morphology. NCT-ME fast-dissolving films were prepared by the solvent casting technique. The films were characterized by morphology, weight, thickness, disintegration time, and mechanical strength properties and the determined NCT loading efficiency and in vitro drug release. The results showed that almost all NCT-MEs presented droplet sizes of less than 100 nm with a spherical form, narrow size distribution, and zeta potentials of −10.6 to −73.7 mV. There was no difference in weight and thickness between all NCT-ME films, but significant changes in the disintegration times were noticed in NCT40-Smix[PEG-40H(2:1)]10 film. The mechanical properties of films varied with changes in type of surfactant. About 80% of the drug release was observed to be between 3 and 30 min. The drug release kinetics were fitted with the Higuchi matrix model. The NCT40-Smix[P-80(1:1)]10 film showed the highest dissolution rate. It was concluded that the developed ME-loaded fast-dissolving film can increase drug release to a greater extent than the films without ME.
- Published
- 2022
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37. Mass Spectrometry-Based Metabolomics of Phytocannabinoids from Non-Cannabis Plant Origins
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Sarana Rose Sommano, Piyachat Sunanta, Noppol Leksawasdi, Kittisak Jantanasakulwong, Pornchai Rachtanapun, Phisit Seesuriyachan, Yuthana Phimolsiripol, Korawan Sringarm, Warintorn Ruksiriwanich, Pensak Jantrawut, and Chuda Chittasupho
- Subjects
Cannabis sativa L. ,cannabinomic ,endocannabinoid ,phytocannabinoids ,mass spectrometry ,metabolite profiling ,Organic chemistry ,QD241-441 - Abstract
Phytocannabinoids are isoprenylated resorcinyl polyketides produced mostly in glandular trichomes of Cannabis sativa L. These discoveries led to the identification of cannabinoid receptors, which modulate psychotropic and pharmacological reactions and are found primarily in the human central nervous system. As a result of the biogenetic process, aliphatic ketide phytocannabinoids are exclusively found in the cannabis species and have a limited natural distribution, whereas phenethyl-type phytocannabinoids are present in higher plants, liverworts, and fungi. The development of cannabinomics has uncovered evidence of new sources containing various phytocannabinoid derivatives. Phytocannabinoids have been isolated as artifacts from their carboxylated forms (pre-cannabinoids or acidic cannabinoids) from plant sources. In this review, the overview of the phytocannabinoid biosynthesis is presented. Different non-cannabis plant sources are described either from those belonging to the angiosperm species and bryophytes, together with their metabolomic structures. Lastly, we discuss the legal framework for the ingestion of these biological materials which currently receive the attention as a legal high.
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- 2022
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38. Development of Carboxymethyl Chitosan Nanoparticles Prepared by Ultrasound-Assisted Technique for a Clindamycin HCl Carrier
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Tanpong Chaiwarit, Sarana Rose Sommano, Pornchai Rachtanapun, Nutthapong Kantrong, Warintorn Ruksiriwanich, Mont Kumpugdee-Vollrath, and Pensak Jantrawut
- Subjects
carboxymethyl chitosan ,experimental design ,nanoparticle ,ultrasound ,clindamycin ,Organic chemistry ,QD241-441 - Abstract
Polymeric nanoparticles are one method to modify the drug release of small hydrophilic molecules. In this study, clindamycin HCl was used as a model drug loaded in carboxymethyl chitosan nanoparticles cross-linked with Ca2+ ions (CMCS-Ca2+). The ultrasonication with experimental design was used to produce CMCS-Ca2+ nanoparticles loading clindamycin HCl. The model showed that the size of nanoparticles decreased when amplitude and time increased. The nanoparticle size of 318.40 ± 7.56 nm, decreased significantly from 543.63 ± 55.07 nm (p < 0.05), was obtained from 75% of amplitude and 180 s of time, which was one of the optimal conditions. The clindamycin loading content in this condition was 34.68 ± 2.54%. The drug content in nanoparticles showed an inverse relationship with the size of the nanoparticles. The sodium carboxymethylcellulose film loading clindamycin HCl nanoparticles exhibited extended release with 69.88 ± 2.03% drug release at 60 min and a gradual increase to 94.99 ± 4.70% at 24 h, and demonstrated good antibacterial activity against S. aureus and C. acne with 40.72 ± 1.23 and 48.70 ± 1.99 mm of the zone of inhibition at 24 h, respectively. Thus, CMCS-Ca2+ nanoparticles produced by the ultrasound-assisted technique could be a potential delivery system to modify the drug release of small hydrophilic antibiotics.
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- 2022
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39. Volatile Organic Compounds from Basil Essential Oils: Plant Taxonomy, Biological Activities, and Their Applications in Tropical Fruit Productions
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Tibet Tangpao, Nutthawut Charoimek, Patipon Teerakitchotikan, Noppol Leksawasdi, Kittisak Jantanasakulwong, Pornchai Rachtanapun, Phisit Seesuriyachan, Yuthana Phimolsiripol, Thanongsak Chaiyaso, Warintorn Ruksiriwanich, Pensak Jantrawut, Hien Van Doan, Ratchadawan Cheewangkoon, and Sarana Rose Sommano
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essential oil ,integrated pest management ,Oriental fruit fly ,pomology ,post-harvest disease control ,Plant culture ,SB1-1110 - Abstract
Basils of the genus Ocimum are aromatic plants grown widely throughout the tropical and temperate regions. The essential oils obtained from their aerial parts are enriched with volatile organic compounds with high market demand for food and pharmaceutical industries. The volatile organic compounds have been shown to exhibit biological activities. Therefore, their novel applications have been extensively explored in the last few decades. The most widely available basils in the tropical areas include white holy basil (O. sanctum var. Shyama), red holy basil (O. sanctum var. Rama), Thai basil (O. basilicum var. thyrsiflorum), lemon basil (O. citriodorum), and tree basil (O. gratissimum). Over 60 volatiles of different classes have been exclusively described, and some of them could be useful as biomarkers for genotype specification. The major volatile ingredient is the phenylpropanoids, such as methyl eugenol, which has the potential as a natural product for mitigating Oriental fruit fly (Bactrocera dorsalis) during tropical fruit production. Moreover, basil essential oils are also used to control diseases of the fruits during post-harvest storage. As a result, the application of basil essential oils as a sustainable defect control strategy for tropical fruit value chains seems intriguing. This review provides comprehensive information on plant taxonomy and volatile compositions of the essential oil fractions from different basil species. Their biological activities and applications are also discussed, mainly during the pre- and post-production of tropical fruits. Additionally, the available techniques to enhance the efficacy of the volatile active compounds are also described.
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- 2022
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40. In Vitro and In Vivo Regulation of SRD5A mRNA Expression of Supercritical Carbon Dioxide Extract from Asparagus racemosus Willd. Root as Anti-Sebum and Pore-Minimizing Active Ingredients
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Warintorn Ruksiriwanich, Chiranan Khantham, Pichchapa Linsaenkart, Tanakarn Chaitep, Pensak Jantrawut, Chuda Chittasupho, Pornchai Rachtanapun, Kittisak Jantanasakulwong, Yuthana Phimolsiripol, Sarana Rose Sommano, Chaiwat Arjin, Houda Berrada, Francisco J. Barba, and Korawan Sringarm
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anti-sebum efficacy ,Asparagus racemosus ,facial-pore-minimizing efficacy ,facial sebum production ,5-alpha reductase enzymes ,human volunteer ,Organic chemistry ,QD241-441 - Abstract
Oily skin from overactive sebaceous glands affects self-confidence and personality. There is report of an association between steroid 5-alpha reductase gene (SRD5A) expression and facial sebum production. There is no study of the effect of Asparagus racemosus Willd. root extract on the regulation of SRD5A mRNA expression and anti-sebum efficacy. This study extracted A. racemosus using the supercritical carbon dioxide fluid technique with ethanol and investigated its biological compounds and activities. The A. racemosus root extract had a high content of polyphenolic compounds, including quercetin, naringenin, and p-coumaric acid, and DPPH scavenging activity comparable to that of the standard L-ascorbic acid. A. racemosus root extract showed not only a significant reduction in SRD5A1 and SRD5A2 mRNA expression by about 45.45% and 90.86%, respectively, but also a reduction in the in vivo anti-sebum efficacy in male volunteers, with significantly superior percentage changes in facial sebum production and a reduction in the percentages of pore area after 15 and 30 days of treatment. It can be concluded that A. racemosus root extract with a high content of polyphenol compounds, great antioxidant effects, promising downregulation of SRD5A1 and SRD5A2, and predominant facial sebum reduction and pore-minimizing efficacy could be a candidate for an anti-sebum and pore-minimizing active ingredient to serve in functional cosmetic applications.
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- 2022
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41. ‘Tablet-in-Syringe’: A Novel Dosing Mechanism for Dysphagic Patients Containing Fast-Disintegrating Tablets Fabricated Using Semisolid Extrusion 3D Printing
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Pattaraporn Panraksa, Bin Zhang, Pornchai Rachtanapun, Kittisak Jantanasakulwong, Sheng Qi, and Pensak Jantrawut
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3D printing ,extrusion-based 3D printing ,semisolid extrusion 3D printing ,dysphagia ,fast-disintegrating tablets ,phenytoin sodium ,Pharmacy and materia medica ,RS1-441 - Abstract
With the ability to fabricate personalized dosage forms and considerably shorter manufacturing time, semisolid extrusion (SSE) 3D printing has rapidly grown in popularity in recent years as a novel, versatile manufacturing method that powers a wide range of applications in the pharmaceutical field. In this work, the feasibility of using SSE 3D printing to fabricate fast-disintegrating tablets (FDTs) that are pre-filled in dosing syringes was evaluated. The novel design approach, ‘tablet-in-syringe’, was aimed to ease the oral drug administration and improve the dosing accuracy for dysphagic patients. The effect of varying polymer (hydroxypropyl methylcellulose E15) concentrations and printing parameters (e.g., extrusion rate) on dimensional accuracy, physicochemical properties, disintegration time, and content uniformity of 3D-printed FDTs was studied. An overall comparison of results demonstrated that the best FDT formulation among those developed was with a polymer:drug ratio (w/w) of 1:30, printed at extrusion rate of 3.5 μL/s. The diameter of printed filaments of this formulation was observed to be similar to the nozzle diameter (22G), proving that good printing accuracy was achieved. This FDTs also had the fastest disintegration time (0.81 ± 0.14 min) and a drug (phenytoin sodium, as the model drug) content uniformity that met pharmacopeial specifications. Although the flow characteristics of the dissolved formulation still need improvement, our findings suggested that the novel ‘tablet-in-syringe’ could potentially be considered as a promising fast-disintegrating drug delivery system that can be personalized and manufactured at—or close to—the point of care for dysphagic patients using SSE.
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- 2022
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42. Morphology, Mechanical, and Water Barrier Properties of Carboxymethyl Rice Starch Films: Sodium Hydroxide Effect
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Pornchai Rachtanapun, Sarinthip Thanakkasaranee, Rafael A. Auras, Nareekan Chaiwong, Kittisak Jantanasakulwong, Pensak Jantrawut, Yuthana Phimolsiripol, Phisit Seesuriyachan, Noppol Leksawasdi, Thanongsak Chaiyaso, Sarana Rose Somman, Warintorn Ruksiriwanich, Warinporn Klunklin, Alissara Reungsang, and Thi Minh Phuong Ngo
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mechanical properties ,morphology ,water vapor permeability ,solubility ,NaOH ,Organic chemistry ,QD241-441 - Abstract
Carboxymethyl rice starch films were prepared from carboxymethyl rice starch (CMSr) treated with sodium hydroxide (NaOH) at 10–50% w/v. The objective of this research was to determine the effect of NaOH concentrations on morphology, mechanical properties, and water barrier properties of the CMSr films. The degree of substitution (DS) and morphology of native rice starch and CMSr powders were examined. Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD), and differential scanning calorimetry (DSC) were used to investigate the chemical structure, crystallinity, and thermal properties of the CMSr films. As the NaOH concentrations increased, the DS of CMSr powders increased, which affected the morphology of CMSr powders; a polyhedral shape of the native rice starch was deformed. In addition, the increase in NaOH concentrations of the synthesis of CMSr resulted in an increase in water solubility, elongation at break, and water vapor permeability (WVP) of CMSr films. On the other hand, the water contact angle, melting temperature, and the tensile strength of the CMSr films decreased with increasing NaOH concentrations. However, the tensile strength of the CMSr films was relatively low. Therefore, such a property needs to be improved and the application of the developed films should be investigated in the future work.
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- 2022
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43. Antioxidation, Anti-Inflammation, and Regulation of SRD5A Gene Expression of Oryza sativa cv. Bue Bang 3 CMU Husk and Bran Extracts as Androgenetic Alopecia Molecular Treatment Substances
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Chiranan Khantham, Pichchapa Linsaenkart, Tanakarn Chaitep, Pensak Jantrawut, Chuda Chittasupho, Pornchai Rachtanapun, Kittisak Jantanasakulwong, Yuthana Phimolsiripol, Sarana Rose Sommano, Chanakan Prom-u-thai, Sansanee Jamjod, Chaiwat Arjin, Korawan Sringarm, Houda Berrada, Francisco J. Barba, Francisco David Carmona, Wutigri Nimlamool, and Warintorn Ruksiriwanich
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androgenetic alopecia ,anti-hair loss ,anti-inflammation ,bioactive compounds ,Oryza sativa ,5α-reductase gene expression ,Botany ,QK1-989 - Abstract
Androgenetic alopecia (AGA), a hair loss disorder, is a genetic predisposition to sensitive androgens, inflammation, and oxidative stress. Unfortunately, current treatments with synthetic medicines contain a restricted mechanism along with side effects, whereas the bioactive constituents of plant extracts are multifunctional, with fewer side effects. The massive amounts of rice husk and bran are agricultural wastes that may cause pollution and environmental problems. Owing to these rationales, the local rice variety, Bue Bang 3 CMU (BB3CMU), which is grown in northern Thailand, was evaluated for the valuable utilization of rice by-products, husk (BB3CMU-H) and bran (BB3CMU-RB) extracts, for AGA treatment regarding antioxidant, anti-inflammatory, anti-androgenic activities, and the characterization of bioactive compounds. Our study verified that BB3CMU-H had the highest level of polyphenols, contributing to its greater antioxidant activity. Conversely, BB3CMU-RB was the predominant source of tocopherols, resulting in better anti-androgenic activities regarding the downregulation of steroid 5α-reductase genes (SRD5A). Notably, anti-inflammation via the attenuation of nitric oxide productions was observed in BB3CMU-H (0.06 ± 0.13 μM) and BB3CMU-RB (0.13 ± 0.01 μM), which were significantly comparable to diclofenac sodium salt (0.13 ± 0.19 μM). Therefore, the combination of BB3CMU-H and BB3CMU-RB could be utilized in cosmeceutical and pharmaceutical applications for AGA patients.
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- 2022
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44. High Efficiency In Vitro Wound Healing of Dictyophora indusiata Extracts via Anti-Inflammatory and Collagen Stimulating (MMP-2 Inhibition) Mechanisms
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Yasir Nazir, Pichchapa Linsaenkart, Chiranan Khantham, Tanakarn Chaitep, Pensak Jantrawut, Chuda Chittasupho, Pornchai Rachtanapun, Kittisak Jantanasakulwong, Yuthana Phimolsiripol, Sarana Rose Sommano, Jiraporn Tocharus, Salin Mingmalairak, Anchali Wongsa, Chaiwat Arjin, Korawan Sringarm, Houda Berrada, Francisco J. Barba, and Warintorn Ruksiriwanich
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anti-inflammatory ,bamboo mushroom ,collagen stimulating activity ,Dictyophora indusiata ,matrix metalloproteinase-2 activity ,wound healing ,Biology (General) ,QH301-705.5 - Abstract
Dictyophora indusiata or Phallus indusiatus is widely used as not only traditional medicine, functional foods, but also, skin care agents. Biological activities of the fruiting body from D. indusiata were widely reported, while the studies on the application of immature bamboo mushroom extracts were limited especially in the wound healing effect. Wound healing process composed of 4 stages including hemostasis, inflammation, proliferation, and remodelling. This study divided the egg stage of bamboo mushroom into 3 parts: peel and green mixture (PGW), core (CW), and whole mushroom (WW). Then, aqueous extracts were investigated for their nucleotide sequencing, biological compound contents, and wound healing effect. The anti-inflammatory determination via the levels of cytokine releasing from macrophages, and the collagen stimulation activity on fibroblasts by matrix metalloproteinase-2 (MMP-2) inhibitory activity were determined to serve for the wound healing process promotion in the stage 2–4 (wound inflammation, proliferation, and remodelling of the skin). All D. indusiata extracts showed good antioxidant potential, significantly anti-inflammatory activity in the decreasing of the nitric oxide (NO), interleukin-1 (IL-1), interleukin-1 (IL-6), and tumour necrosis factor-α (TNF-α) secretion from macrophage cells (p < 0.05), and the effective collagen stimulation via MMP-2 inhibition. In particular, CW extract containing high content of catechin (68.761 ± 0.010 mg/g extract) which could significantly suppress NO secretion (0.06 ± 0.02 µmol/L) better than the standard anti-inflammatory drug diclofenac (0.12 ± 0.02 µmol/L) and their MMP-2 inhibition (41.33 ± 9.44%) was comparable to L-ascorbic acid (50.65 ± 2.53%). These findings support that CW of D. indusiata could be an essential natural active ingredient for skin wound healing pharmaceutical products.
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- 2021
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45. Composite Nanocellulose Fibers-Based Hydrogels Loading Clindamycin HCl with Ca2+ and Citric Acid as Crosslinking Agents for Pharmaceutical Applications
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Pichapar O-chongpian, Mingkwan Na Takuathung, Chuda Chittasupho, Warintorn Ruksiriwanich, Tanpong Chaiwarit, Phornsawat Baipaywad, and Pensak Jantrawut
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nanocellulose fiber ,hydrogel ,low methoxyl pectin ,sodium alginate ,clindamycin ,Organic chemistry ,QD241-441 - Abstract
Biocomposite hydrogels based on nanocellulose fibers (CNFs), low methoxy pectin (LMP), and sodium alginate (SA) were fabricated via the chemical crosslinking technique. The selected CNFs-based hydrogels were loaded with clindamycin hydrochloride (CM), an effective antibiotic as a model drug. The properties of the selected CNFs-based hydrogels loaded CM were characterized. The results showed that CNFs-based hydrogels composed of CNFs/LMP/SA at 1:1:1 and 2:0.5:0.5 mass ratios exhibited high drug content, suitable gel content, and high maximum swelling degree. In vitro assessment of cell viability revealed that the CM-incorporated composite CNFs-based hydrogels using calcium ion and citric acid as crosslinking agents exhibited high cytocompatibility with human keratinocytes cells. In vitro drug release experiment showed the prolonged release of CM and the hydrogel which has a greater CNFs portion (C2P0.5A0.5/Ca + Ci/CM) demonstrated lower drug release than the hydrogel having a lesser CNFs portion (C1P1A1/Ca + Ci/CM). The proportion of hydrophilic materials which were low methoxy pectin and sodium alginate in the matrix system influences drug release. In conclusion, biocomposite CNFs-based hydrogels composed of CNFs/LMP/SA at 1:1:1 and 2:0.5:0.5 mass ratios, loading CM with calcium ion and citric acid as crosslinking agents were successfully developed for the first time, suggesting their potential for pharmaceutical applications, such as a drug delivery system for healing infected wounds.
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- 2021
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46. Effect of Egg-Coating Material Properties by Blending Cassava Starch with Methyl Celluloses and Waxes on Egg Quality
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Pornchai Rachtanapun, Nattagarn Homsaard, Araya Kodsangma, Noppol Leksawasdi, Yuthana Phimolsiripol, Suphat Phongthai, Julaluk Khemacheewakul, Phisit Seesuriyachan, Thanongsak Chaiyaso, Suwit Chotinan, Pensak Jantrawut, Warintorn Ruksiriwanich, Sutee Wangtueai, Sarana Rose Sommano, Wirongrong Tongdeesoontorn, and Kittisak Jantanasakulwong
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Manihot esculenta ,carboxymethyl cellulose ,paraffin ,Haugh unit ,weight loss ,freshness ,Organic chemistry ,QD241-441 - Abstract
An egg-coating material was developed to extend the shelf-life and freshness of eggs by blending cassava starch (CS) with gelling agents and waxes. The effects of the properties of this egg coating on egg quality were investigated. Hydroxypropyl methylcellulose (HPMC), carboxymethyl cellulose (CMC), beeswax, and paraffin wax were used. CS blended with low-molecular-weight paraffin (Paraffin(L)) and CMC coating material displayed a tensile strength of 4 MPa, 34% elongation at break, 0.0039 g day−1 m−2 water vapor permeability, and a water contact angle of 89° at 3 min. Eggs coated with CS/CMC/Paraffin(L) solutions had a Haugh unit value of 72 (AA grade) and exhibited a weight loss of 2.4% in 4 weeks. CMC improved the compatibility of CS and Paraffin(L). This improvement and the hydrophobicity of Paraffin(L) provided suitable mechanical and water-resistance properties to the coating material that helped to maintain the quality of the coated AA-grade eggs with low weight loss for 4 weeks.
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- 2021
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47. Mango Peel Pectin: Recovery, Functionality and Sustainable Uses
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Malaiporn Wongkaew, Pikulthong Chaimongkol, Noppol Leksawasdi, Kittisak Jantanasakulwong, Pornchai Rachtanapun, Phisit Seesuriyachan, Yuthana Phimolsiripol, Thanongsak Chaiyaso, Warintorn Ruksiriwanich, Pensak Jantrawut, and Sarana Rose Sommano
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extraction technique ,fruit characteristic ,mango peel biorefinery ,pectic polysaccharide ,pectin source ,Organic chemistry ,QD241-441 - Abstract
Concerns regarding the overconsumption of natural resources has provoked the recovery of biopolymers from food processing biomass. Furthermore, the current market opportunity for pectin in other areas has increased, necessitating the search for alternative pectin resources. This is also a step towards the sustainable and circular green economy. Mango peel is the byproduct of agro-processing and has been used for high value-added components such as polysaccharide biopolymers. Pectin derived from the peel is yet to be exploited to its greatest extent, particularly in terms of its separation and physiochemical properties, which limit its applicability to dietary fiber in culinary applications. The functionality of the mango peel pectin (MPP) strongly depends on the molecular size and degree of esterification which highlight the importance of isolation and characterisation of pectin from this novel resource. This article therefore provides a useful overview of mango peel as a potential biomaterial for the recovery of MPP. Different extraction techniques and the integrated recovery were also discussed. The utilisation of MPP in different industrial schemes are also detailed out from different perspectives such as the pharmaceutical and biotechnology industries. This review convincingly expresses the significance of MPP, providing a sustainable opportunity for food and pharmaceutical development.
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- 2021
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48. High Substitution Synthesis of Carboxymethyl Chitosan for Properties Improvement of Carboxymethyl Chitosan Films Depending on Particle Sizes
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Sarinthip Thanakkasaranee, Kittisak Jantanasakulwong, Yuthana Phimolsiripol, Noppol Leksawasdi, Phisit Seesuriyachan, Thanongsak Chaiyaso, Pensak Jantrawut, Warintorn Ruksiriwanich, Sarana Rose Sommano, Winita Punyodom, Alissara Reungsang, Thi Minh Phuong Ngo, Parichat Thipchai, Wirongrong Tongdeesoontorn, and Pornchai Rachtanapun
- Subjects
chitosan ,carboxymethyl chitosan ,particle size ,water solubility ,degree of substitution ,Organic chemistry ,QD241-441 - Abstract
This study investigated the effect of chitosan particle sizes on the properties of carboxymethyl chitosan (CMCh) powders and films. Chitosan powders with different particle sizes (75, 125, 250, 450 and 850 µm) were used to synthesize the CMCh powders. The yield, degree of substitution (DS), and water solubility of the CMCh powders were then determined. The CMCh films prepared with CMCh based on chitosan with different particle sizes were fabricated by a solution casting technique. The water solubility, mechanical properties, and water vapor transmission rate (WVTR) of the CMCh films were measured. As the chitosan particle size decreased, the yield, DS, and water solubility of the synthesized CMCh powders increased. The increase in water solubility was due to an increase in the polarity of the CMCh powder, from a higher conversion of chitosan into CMCh. In addition, the higher conversion of chitosan was also related to a higher surface area in the substitution reaction provided by chitosan powder with a smaller particle size. As the particle size of chitosan decreased, the tensile strength, elongation at break, and WVTR of the CMCh films increased. This study demonstrated that a greater improvement in water solubility of the CMCh powders and films can be achieved by using chitosan powder with a smaller size.
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- 2021
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49. Characterization of Hydrophilic Polymers as a Syringe Extrusion 3D Printing Material for Orodispersible Film
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Pattaraporn Panraksa, Sheng Qi, Suruk Udomsom, Pratchaya Tipduangta, Pornchai Rachtanapun, Kittisak Jantanasakulwong, and Pensak Jantrawut
- Subjects
syringe extrusion 3D printing ,orodispersible film ,hydrophilic polymer ,disintegration ,Organic chemistry ,QD241-441 - Abstract
The application of hydrophilic polymers in designing and three-dimensional (3D) printing of pharmaceutical products in various dosage forms has recently been paid much attention. Use of hydrophilic polymers and syringe extrusion 3D printing technology in the fabrication of orodispersible films (ODFs) might hold great potential in rapid drug delivery, personalized medicine, and manufacturing time savings. In this study, the feasibility of 3D-printed ODFs fabrication through a syringe extrusion 3D printing technique and using five different hydrophilic polymers (e.g., hydroxypropyl methylcellulose E15, hydroxypropyl methylcellulose E50, high methoxyl pectin, sodium carboxymethylcellulose, and hydroxyethylcellulose) as film-forming polymers and printing materials has been investigated. Rheology properties and printability of printing gels and physicochemical and mechanical properties of 3D-printed ODFs were evaluated. Amongst the investigated hydrophilic polymers, sodium carboxymethylcellulose at a concentration of 5% w/v (SCMC-5) showed promising results with a good printing resolution and accurate dimensions of the 3D-printed ODFs. In addition, SCMC-5 3D-printed ODFs exhibited the fastest disintegration time within 3 s due to high wettability, roughness and porosity on the surface. However, the results of the mechanical properties study showed that SCMC-5 3D printed ODFs were rigid and brittle, thus requiring special packaging to prevent them from any damage before practical use.
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- 2021
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50. Biopolymer Hydrogel Scaffolds Containing Doxorubicin as A Localized Drug Delivery System for Inhibiting Lung Cancer Cell Proliferation
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Chuda Chittasupho, Jakrapong Angklomklew, Thanu Thongnopkoon, Wongwit Senavongse, Pensak Jantrawut, and Warintorn Ruksiriwanich
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gelatin ,sodium carboxymethyl cellulose ,scaffold ,A549 cells ,freeze drying ,Organic chemistry ,QD241-441 - Abstract
A hydrogel scaffold is a localized drug delivery system that can maintain the therapeutic level of drug concentration at the tumor site. In this study, the biopolymer hydrogel scaffold encapsulating doxorubicin was fabricated from gelatin, sodium carboxymethyl cellulose, and gelatin/sodium carboxymethyl cellulose mixture using a lyophilization technique. The effects of a crosslinker on scaffold morphology and pore size were determined using scanning electron microscopy. The encapsulation efficiency and the release profile of doxorubicin from the hydrogel scaffolds were determined using UV-Vis spectrophotometry. The anti-proliferative effect of the scaffolds against the lung cancer cell line was investigated using an MTT assay. The results showed that scaffolds made from different types of natural polymer had different pore configurations and pore sizes. All scaffolds had high encapsulation efficiency and drug-controlled release profiles. The viability and proliferation of A549 cells, treated with gelatin, gelatin/SCMC, and SCMC scaffolds containing doxorubicin significantly decreased compared with control. These hydrogel scaffolds might provide a promising approach for developing a superior localized drug delivery system to kill lung cancer cells.
- Published
- 2021
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