Search

Analysis of the ATP levels, fatty acid compositions and protein profiles during the transition from the spiral to the coccoid form of the Campylobacter jejuni, indicate that the cocci formation is not an effective process. The cocci formed at 4 degree centigrade and the spiral form participate in the contamination cycle of C. jejuni. At low temperatures the fatty acid composition of cocci and spiral forms are same, the levels of intracellular ATP are highest and there is no change in the protein profiles.

Enzyme-linked immunosorbent assay (ELISA) is the most commonly used serological diagnostic technique. A number of different ELISA formats can be used for the detection of bacterial plant pathogens and in particular Erwinia amylovora and Clavibacter michiganensis subsp. sepedonicus.

Immunofluorescence microscopy is a very sensitive serological test which harnesses both the power of antibodies to bind to targets along with the use of the fluorescence microscope to visualise the structures to which they bind. Antibody binding is visualised by the fluorescent emission from a marker molecule bound to the antibody. The technique is of particular use in bacteriology and pathology where the location and morphology of the bacterial cells can be viewed along with the location of the fluorescently labelled antibodies.