Search

Your search keyword '"Janny, Laurent"' showing total 33 results
Start Over Author "Janny, Laurent"
33 results on '"Janny, Laurent"'

10. CFTR p.Arg117His associated with CBAVD and other CFTR-related disorders

Author

Thauvin-Robinet, Christel, Munck, Anne, Huet, Frédéric, de Becdelièvre, Alix, Jimenez, Clément, Lalau, Guy, Gautier, Elodie, Rollet, Jacques, Flori, Jean, Nové-Josserand, Raphaëlle, Soufir, Jean-Claude, Haloun, Alain, Hubert, Dominique, Houssin, Elise, Bellis, Gil, Rault, Gilles, David, Albert, Janny, Laurent, Chiron, Raphaël, Rives, Nathalie, Hairion, Dominique, Collignon, Patrick, Valeri, Antoine, Karsenty, Gilles, Rossi, Annick, Audrézet, Marie-Pierre, Férec, Claude, Leclerc, Julie, Georges, Marie des, Claustres, Mireille, Bienvenu, Thierry, Gérard, Bénédicte, Boisseau, Pierre, Cabet-Bey, Faïza, Cheillan, David, Feldmann, Delphine, Clavel, Christine, Bieth, Eric, Iron, Albert, Simon-Bouy, Brigitte, Izard, Vincent, Steffann, Julie, Viville, Stéphane, Costa, Catherine, Drouineaud, Véronique, Fauque, Patricia, Binquet, Christine, Bonithon-Kopp, Claire, Morris, Mike A, Faivre, Laurence, Goossens, Michel, Roussey, Michel, Girodon, Emmanuelle, Bazin, A, Blayau, M, Bonnefont, JP, Bouligand, J, Chéry, M, Chevalier-Porst, F, Costa, JM, Coude, M, Creveaux, I, Dalstein, V, Gerson, F, Gobin-Limballe, S, Gouget, AM, Kitzis, A, Lagier-Tourenne, C, Magdelaine, C, Malinge, MC, Malzac, P, Mittre, H, Petit, V, Philippe, C, Ray, P, Raynaud, M, Ronsin, C, Schmitt, S, Albert, M, Bassinet, L, Bellon, G, Bonnefoy, S, Bourouillou, G, Bremont, F, Brechard, MP, Chardot, C, Chevalier, MC, Chiesa, J, Ciolkovitch, A, Clement, A, Corvol, H, Counil, F, Costa, P, David, V, Delacourt, C, Delafontaine, D, Delepoulle, F, Delrue, MA, Deneuville, E, Derelle, J, Desrues, B, Dominique, S, Fanton, AL, Foucaud, P, Freour, T, Froment, S, Gaillard, D, Gérardin, M, Giacomini, P, Gambert, C, Gautier, E, Ginies, JL, Ginglinger, E, Gottrand, F, Guichet, A, Guillot, M, Heraud, MC, Houriez-Bertolo, E, Jeandidier, E, Journel, H, Labarière, Lahsinat, K, Langlais, S, Languepin, J, Laurans, M, Lauton, D, Layet, V, Le Bourgeois, M, Le Lannou, D, Lejeune, H, Lenoir, G, Leroy, S, Lestrade, F, Llerena, C, Marc, B, Marchand, S, Marguet, C, Marteletti, O, Massat, G, Masurel-Paulet, A, Mely, L, Menetrey, C, Moisan-Petit, V, Montcouquiol, S, Moreau, L, Odent, S, Pagenault, M, Parent, P, Pautard, JC, Perez-Martin, S, Peter, MO, Pierre, D, Pin, I, Plessis, G, Ramel, S, Rembert-Sagot, F, Roux, C, Royere, D, Sardet, A, Sarles, J, Sermet-Gaudelus, I, Siffroi, JP, Saulnier, JP, Sehabiague, J, Sinet, PM, Tassin, E, Terro, F, Turck, D, Vodoff, MV, Wagner, L, and Weiss, L

Published
2013
Full Text
View/download PDF

11. Liste des auteurs

Author

Elodie, Adda-Herzog, Louise, Adjiman, Naouel, Ahdad-Yata, Sylvia, Alvarez, Jean-Marie, Antoine, Christophe, Arnoult, Paul, Atlan, Jean-Marc, Ayoubi, Paul, Barrière, Alexandre, Bellucci, Achraf, Benammar, Marion, Bendayan, Moncef, Benkhalifa, Valérie, Bernard, Florence, Boitrelle, Corrine, Bordonné, Philippe, Bouchard, Yasmine, Boumerdassi, Charlotte, Bourdin, Mathilde, Bourdon, Rosalie, Cabry, Perrine, Capmas, Marie, Carbonnel, Isabelle, Cedrin-Durnerin, Charles, Chapron, Ahmed, Chargui, Sophie, Christin-Maitre, Jocelyne, De Laveaucoupet, Dominique, de Ziegler, Elodie, Debras, Christine, Decanter, Jacques, Demouzon, Didier, Dewailly, Gauthier, Dietrich, Ludovic, Dumont, Sylvie, Epelboin, Florence, Eustache, Marc, Even, Renato, Fanchin, Patricia, Fauque, Stéphanie, Fay, Aurélie, Feraille, Hervé, Fernandez, Xavier, Ferraretto, Lucile, Ferreux, Meriem, Filali, Julie, Firmin, Muriel, Flis-Trèves, Camille, Fossard, Thomas, Freour, René, Frydman, Michael, Grynberg, Camille, Grysole, Véronique, Guegan, Samir, Hamamah, Estelle, Heggarty, Hesters, Laetitia, Vincent, Izard, Laetitia, Jacquesson, Margaux, Jegaden, Léa, Karpel, Julie, Labrosse, Tatiana, Lecot-Connan, Elodie, Lefranc, Hélène, Letur, Jean-Marc, Levaillant, Marie-Astrid, Llabador, Noureddine, Louanjli, Sophie, Loubersac, Chloé, Maignien, Moudou, Mamoune Mbaye, Louis, Marcellin, Nathalie, Massin, d'Argent Emmanuelle, Mathieu, Marie-Laure, Maurin, Anne, Mayeur, Charlotte, Methorst, Anne Élodie, Millischer, Pierre, Miron, Debbie, Montjean, Arnold, Munnich, Jana, Muroňová, François, Olivennes, Anne, Oppenheimer, Catherine, Patrat, Sarah, Peyrelevade, Olivier, Pirrello, Paul, Pirtea, Khaled, Pocate-Cheriet, Xavier, Pollet-Villard, Marine, Poulain, Anne-Gaëlle, Pourcelot, Julien, Puntonet, Pierre, Ray, Arnaud, Reignier, Virginie, Rio, Nathalie, Rives, Catherine, Rongières, Pietro, Santulli, Inès, Sellami, Lise, Selleret, Christophe, Sifer, Charlotte, Sonigo, Julie, Steffann, Myriam, Szejer, Charles, Tibi, Jessica, Vandame, Yves, Ville, Jonathan, Zerbib, Achour-Frydman, Nelly, Alter, Laura, Hoffet, Aurélie Amar, Avril, Catherine, Belaisch-Allart, Joëlle, Belhadrie-Mansouri, Naima, Bergere, Marianne, Boistot, Lucile, Boyer, Pierre, Brugnon, Florence, Catteau, Aurore, Chauffour, Candice, Celton, Noémie, Copin, Henri, Dejou-Bouillet, Lydie, Deutsch-Bringer, Sophie, Devaux, Aviva, Dolto, Catherine, Dumont, Martine, Duros, Solène, Ferraretto, Xavier, Ferretti, Caterina, Gayet, Vanessa, Gervoise-Boyer, Marie, Giguère, Claudie, Gremeau, Anne-Sophie, Gronier, Héloïse, Guérin, Jean-François, Haouzi, Delphine, Hédon, Bernard, Hugues, Jean-Noël, Janny, Laurent, Kadoch, Isaac-Jacques, Le Bras-Mayeur, Anne, Lédée, Nathalie, Lemoine, Mathilde, Lévy, Rachel, de Royer, Marie-Astrid Llabador, Lornage, Jacqueline, Lubin, Vanessa, Marzouk, Paul, Merviel, Philippe, Mestres, Stéphanie, Molina-Gomes, Denise, Montagut, Jacques, Nataf, Eric, Oger, Pierre, Pouly, Jean-Luc, Rayssiguier, Romy, Rossin, Betty, Salle, Bruno, Scalici, Elodie, Selva, Jacqueline, Siraudin, Cendrine, Splingart, Carole, Torre, Antoine, Valière, Martine, Vialard, François, Vinolas, Claire, and Yazbeck, Chadi

Published
2023
Full Text
View/download PDF

14. Liver X Receptors (LXRs) Alpha and Beta Play Distinct Roles in the Mouse Epididymis1

Author

Whitfield, Marjorie, Ouvrier, Aurélia, Cadet, Rémi, Damon-Soubeyrand, Christelle, Guiton, Rachel, Janny, Laurent, Kocer, Ayhan, Marceau, Geoffroy, Pons-Rejraji, Hanae, Trousson, Amalia, Drevet, Joël R, Saez, Fabrice, Drevet, Joël R., Clermont Université, Génétique, Reproduction et Développement - Clermont Auvergne (GReD), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Clermont Auvergne (UCA)-Centre National de la Recherche Scientifique (CNRS), Biologie du Développement et Reproduction (BDR), Institut National de la Recherche Agronomique (INRA), CHU Clermont-Ferrand, Régulation de la transcription et maladies génétiques (RTMG), Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), Génétique, Reproduction et Développement (GReD), Centre National de la Recherche Scientifique (CNRS)-Université Clermont Auvergne [2017-2020] (UCA [2017-2020])-Institut National de la Santé et de la Recherche Médicale (INSERM), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Clermont Auvergne [2017-2020] (UCA [2017-2020])-Centre National de la Recherche Scientifique (CNRS), and Centre National de la Recherche Scientifique (CNRS)

Subjects
0301 basic medicine, Gene isoform, medicine.medical_specialty, Transgene, [SDV]Life Sciences [q-bio], Biology, cholesterol homeostasis, lipids, 03 medical and health sciences, 0302 clinical medicine, Capacitation, spermatozoa, Internal medicine, medicine, Liver X receptor, 030219 obstetrics & reproductive medicine, Spermatozoon, urogenital system, Lipid metabolism, Cell Biology, General Medicine, Epididymis, Sperm, 030104 developmental biology, medicine.anatomical_structure, Endocrinology, Reproductive Medicine, inflammation, lipids (amino acids, peptides, and proteins), LXR, epididymis
Abstract

International audience; After its production in the testis, a spermatozoon has to undergo posttesticular maturation steps to become fully motile and fertile. The first step is epididymal maturation, during which immature spermatozoa are transformed into biochemically mature cells ready to proceed to the next step, capacitation, a physiological process occurring in the female genital tract. The biochemical transformations include modification of sperm lipid composition during epididymal transit, with significant changes in fatty acids, phospholipids, and sterols between the caput and the cauda epididymal spermatozoa. Although quantitative aspects of these changes are well documented for several mammalian species, molecular mechanisms governing these steps are poorly understood. Transgenic male mice invalidated for the two liver X receptors (LXRalpha and LXRbeta, nuclear oxysterol receptors regulating cholesterol and lipid metabolism) become sterile when aging, showing an epididymal phenotype. We used single-knockout-model mice to characterize the role of each LXR isoform during sperm maturation in the epididymis. We show here that although a certain redundancy exists in the functions of the two LXR isoforms, some physiological processes are more under the influence of only one of them. In both cases, aging males showed slight subfertility, associated with dyslipidemia, emphasizing the importance of lipid metabolism in relation with male fertility.

Published
2016
Full Text
View/download PDF

17. Epididymis Response Partly Compensates for Spermatozoa Oxidative Defects in snGPx4 and GPx5 Double Mutant Mice

Author

Noblanc, Anaïs, Peltier, Manon, Damon-Soubeyrand, Christelle, Kerchkove, Nicolas, Chabory, Eléonore, Vernet, Patrick, Saez, Fabrice, Cadet, Rémi, Janny, Laurent, Pons-Rejraji, Hanae, Conrad, Marcus, Drevet, Joël, Kocer, Ayhan, Bishop, Alexander James Roy, Génétique, Reproduction et Développement (GReD), Centre National de la Recherche Scientifique (CNRS)-Université Clermont Auvergne [2017-2020] (UCA [2017-2020])-Institut National de la Santé et de la Recherche Médicale (INSERM), Réparation du Génome Mitochondrial Normal et Pathologique (RGM), Université Blaise Pascal - Clermont-Ferrand 2 (UBP), Clermont Université, Pharmacy, Biologie du développement et reproduction (BDR), École nationale vétérinaire d'Alfort (ENVA)-Institut National de la Recherche Agronomique (INRA)-Centre National de la Recherche Scientifique (CNRS), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Clermont Auvergne [2017-2020] (UCA [2017-2020])-Centre National de la Recherche Scientifique (CNRS), Centre National de la Recherche Scientifique (CNRS)-École nationale vétérinaire d'Alfort (ENVA)-Institut National de la Recherche Agronomique (INRA), École nationale vétérinaire - Alfort (ENVA)-Institut National de la Recherche Agronomique (INRA)-Centre National de la Recherche Scientifique (CNRS), Génétique, Reproduction et Développement - Clermont Auvergne (GReD), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Clermont Auvergne (UCA)-Centre National de la Recherche Scientifique (CNRS), Biologie du Développement et Reproduction (BDR), and Institut National de la Recherche Agronomique (INRA)

Subjects
Male, Anatomy and Physiology, Mouse, [SDV]Life Sciences [q-bio], lcsh:Medicine, GPX4, Biochemistry, GPX5, chemistry.chemical_compound, Mice, 0302 clinical medicine, Reproductive Physiology, Molecular Cell Biology, Phospholipid-hydroperoxide glutathione peroxidase, lcsh:Science, ComputingMilieux_MISCELLANEOUS, Epididymis, chemistry.chemical_classification, 0303 health sciences, metabolism [Spermatozoa], 030219 obstetrics & reproductive medicine, Multidisciplinary, Glutathione peroxidase, Animal Models, Spermatozoa, Oxygen Metabolism, Signaling Cascades, Cell biology, medicine.anatomical_structure, Medicine, DNA fragmentation, Cellular Types, Thioredoxin, physiology [Epididymis], Research Article, Signal Transduction, Urology, genetics [Glutathione Peroxidase], Mice, Transgenic, Biology, Stress Signaling Cascade, 03 medical and health sciences, Model Organisms, physiology [Spermatozoa], medicine, Animals, ddc:610, 030304 developmental biology, Glutathione Peroxidase, lcsh:R, Reproductive System, selenium-independent glutathione peroxidase, [SDV.BDLR]Life Sciences [q-bio]/Reproductive Biology, Phospholipid Hydroperoxide Glutathione Peroxidase, Sperm, Mice, Mutant Strains, Oxidative Stress, Metabolism, Germ Cells, chemistry, Infertility, Immunology, lcsh:Q
Abstract

We report here that spermatozoa of mice lacking both the sperm nucleus glutathione peroxidase 4 (snGPx4) and the epididymal glutathione peroxidase 5 (GPx5) activities display sperm nucleus structural abnormalities including delayed and defective nuclear compaction, nuclear instability and DNA damage. We show that to counteract the GPx activity losses, the epididymis of the double KO animals mounted an antioxydant response resulting in a strong increase in the global H(2)O(2)-scavenger activity especially in the cauda epididymis. Quantitative RT-PCR data show that together with the up-regulation of epididymal scavengers (of the thioredoxin/peroxiredoxin system as well as glutathione-S-transferases) the epididymis of double mutant animals increased the expression of several disulfide isomerases in an attempt to recover normal disulfide-bridging activity. Despite these compensatory mechanisms cauda-stored spermatozoa of double mutant animals show high levels of DNA oxidation, increased fragmentation and greater susceptibility to nuclear decondensation. Nevertheless, the enzymatic epididymal salvage response is sufficient to maintain full fertility of double KO males whatever their age, crossed with young WT female mice.

Published
2012
Full Text
View/download PDF

19. Evaluation of atorvastatin efficacy and toxicity on spermatozoa, accessory glands and gonadal hormones of healthy men: a pilot prospective clinical trial

Author

Pons-Rejraji, Hanae, primary, Brugnon, Florence, additional, Sion, Benoit, additional, Maqdasy, Salwan, additional, Gouby, Gerald, additional, Pereira, Bruno, additional, Marceau, Geoffroy, additional, Gremeau, Anne-Sophie, additional, Drevet, Joel, additional, Grizard, Genevieve, additional, Janny, Laurent, additional, and Tauveron, Igor, additional

Published
2014
Full Text
View/download PDF

20. Liste des collaborateurs

Author

Achour-Frydman, Nelly, Ahdad-Yata, Naouel, Alter, Laura, Alvarez, Silvia, Hoffet, Aurélie Amar, Antoine, Jean-Marie, Atlan, Paul, Avril, Catherine, Ayoubi, Jean-Marc, Barrière, Paul, Belaisch-Allart, Joëlle, Belhadrie-Mansouri, Naima, Benammar, Achraf, Benkhalifa, Moncef, Bergere, Marianne, Boistot, Lucile, Boitrelle, Florence, Bouchard, Philippe, Bourdon, Mathilde, Boyer, Pierre, Brugnon, Florence, Capmas, Perrine, Catteau, Aurore, Chapron, Charles, Chauffour, Candice, Celton, Noémie, Copin, Henri, Decanter, Christine, Dejou-Bouillet, Lydie, Deutsch-Bringer, Sophie, Devaux, Aviva, Dewailly, Didier, Dolto, Catherine, Dumont, Martine, Duros, Solène, Epelboin, Sylvie, Fanchin, Renato, Fay, Stéphanie, Fernandez, Hervé, Ferraretto, Xavier, Ferretti, Caterina, Flis-Trèves, Muriel, Frydman, René, Gayet, Vanessa, Gervoise-Boyer, Marie, Giguère, Claudie, Gremeau, Anne-Sophie, Gronier, Héloïse, Grynberg, Michaël, Grysole, Camille, Guérin, Jean-François, Hamamah, Samir, Haouzi, Delphine, Hédon, Bernard, Hesters, Laetitia, Hugues, Jean-Noël, Izard, Vincent, Janny, Laurent, Kadoch, Isaac-Jacques, Karpel, Léa, Le Bras-Mayeur, Anne, Lédée, Nathalie, Lemoine, Mathilde, Letur, Hélène, Rachel, Lévy, de Royer, Marie-Astrid Llabador, Lornage, Jacqueline, Lubin, Vanessa, Marcellin, Louis, Marzouk, Paul, Mathieu d'Argent, Emmanuelle, Maurin, Marie-Laure, Merviel, Philippe, Mestres, Stéphanie, Methorst, Charlotte, Molina-Gomes, Denise, Montagut, Jacques, Montjean, Debbie, Nataf, Eric, Oger, Pierre, Olivennes, François, Oppenheimer, Anne, Patrat, Catherine, Peyrelevade, Sarah, Pirrello, Olivier, Pirtea, Paul, Pocate Cheriet, Khaled-Razvan, Pollet-Villard, Xavier, Poulain, Marine, Pouly, Jean-Luc, Pourcelot, Anne-Gaëlle, Rayssiguier, Romy, Rongieres, Catherine, Rossin, Betty, Salle, Bruno, Santulli, Pietro, Scalici, Elodie, Selleret, Lise, Selva, Jacqueline, Sifer, Christophe, Siraudin, Cendrine, Sonigo, Charlotte, Splingart, Carole, Steffann, Julie, Tibi, Charles, Torre, Antoine, Valière, Martine, Vialard, François, Vinolas, Claire, Yazbeck, Chadi, and de Ziegler, Dominique

Published
2016
Full Text
View/download PDF

21. Indoleamine 2,3-Dioxygenase 1 (Ido1) Is Involved in the Control of Mouse Caput Epididymis Immune Environment

Author

Jrad-Lamine, Aicha, primary, Henry-Berger, Joelle, additional, Damon-Soubeyrand, Christelle, additional, Saez, Fabrice, additional, Kocer, Ayhan, additional, Janny, Laurent, additional, Pons-Rejraji, Hanae, additional, Munn, David H., additional, Mellor, Andrew L., additional, Gharbi, Najoua, additional, Cadet, Rémi, additional, Guiton, Rachel, additional, Aitken, Robert J., additional, and Drevet, Joël R., additional

Published
2013
Full Text
View/download PDF

23. Correction: Epididymis Response Partly Compensates for Spermatozoa Oxidative Defects in snGPx4 and GPx5 Double Mutant Mice

Author

Noblanc, Anaïs, primary, Peltier, Manon, additional, Damon-Soubeyrand, Christelle, additional, Kerckhove, Nicolas, additional, Chabory, Eléonore, additional, Vernet, Patrick, additional, Saez, Fabrice, additional, Cadet, Rémi, additional, Janny, Laurent, additional, Pons-Rejraji, Hanae, additional, Conrad, Marcus, additional, Drevet, Joël R., additional, and Kocer, Ayhan, additional

Published
2012
Full Text
View/download PDF

24. Dietary Cholesterol-Induced Post-Testicular Infertility

Author

Ouvrier, Aurélia, primary, Alves, Georges, additional, Damon-Soubeyrand, Christelle, additional, Marceau, Geoffroy, additional, Cadet, Rémi, additional, Janny, Laurent, additional, Brugnon, Florence, additional, Kocer, Ayhan, additional, Pommier, Aurélien, additional, Lobaccaro, Jean-Marc A., additional, Drevet, Joël R., additional, and Saez, Fabrice, additional

Published
2011
Full Text
View/download PDF

25. Outcome of intracytoplasmic sperm injection for a couple in which the man is carrier of CFTR p.[R74W;V201M;D1270N] and p.P841R mutations and his spouse a heterozygous carrier of p.F508del mutation of the cystic fibrosis transmembrane conductance regulator gene

Author

Brugnon, Florence, primary, Bilan, Frederic, additional, Heraud, Marie-Christine, additional, Grizard, Genevieve, additional, Janny, Laurent, additional, and Creveaux, Isabelle, additional

Published
2008
Full Text
View/download PDF

30. Principes de préparation et de congélation des spermatozoïdes testiculaires humains.

Author

Grizard, Geneviève, Force, André, Janny, Laurent, and Boucher, Daniel

Abstract

Copyright of Andrologie (11662654) is the property of Springer Nature and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Published
2003
Full Text
View/download PDF

31. Gamete intrafallopian transfer: benefits of programmed stimulation

Author

Pouly, Jean Luc, Janny, Laurent, Canis, Michel, Vye, Pascale, and Boyer, Christine

Abstract

Fifty-six cycles of gamete intrafallopian transfer (GIFT) were performed after programming by administration of norethisterone in the previous cycle. Ovarian hyperstimulation was achieved with Clomifene citrate and human menopausal gonadotropins. Only one GIFT was performed during a weekend (1.8%). The implantation rate was 41.1% and the evolutive pregnancy rate 30.4%. The endocrinological influence of these regimens are discussed by comparison of those observed in an in vitro fertilization program. The main influence is a decreased serum luteinizing hormone (LH) level in the preovulatory phase.

Published
1989
Full Text
View/download PDF

32. Liver X Receptors (LXRs) Alpha and Beta Play Distinct Roles in the Mouse Epididymis.

Author

Whitfield M, Ouvrier A, Cadet R, Damon-Soubeyrand C, Guiton R, Janny L, Kocer A, Marceau G, Pons-Rejraji H, Trousson A, Drevet JR, and Saez F

Subjects
Aging, Animals, Cholesterol metabolism, Epididymis pathology, Female, Homeostasis, Infertility, Male genetics, Infertility, Male metabolism, Lipid Metabolism, Liver X Receptors genetics, Male, Mice, Mice, Knockout, Pregnancy, Pregnancy Rate, Protein Isoforms, Epididymis metabolism, Gene Expression Regulation physiology, Liver X Receptors metabolism
Abstract

After its production in the testis, a spermatozoon has to undergo posttesticular maturation steps to become fully motile and fertile. The first step is epididymal maturation, during which immature spermatozoa are transformed into biochemically mature cells ready to proceed to the next step, capacitation, a physiological process occurring in the female genital tract. The biochemical transformations include modification of sperm lipid composition during epididymal transit, with significant changes in fatty acids, phospholipids, and sterols between the caput and the cauda epididymal spermatozoa. Although quantitative aspects of these changes are well documented for several mammalian species, molecular mechanisms governing these steps are poorly understood. Transgenic male mice invalidated for the two liver X receptors (LXRalpha and LXRbeta, nuclear oxysterol receptors regulating cholesterol and lipid metabolism) become sterile when aging, showing an epididymal phenotype. We used single-knockout-model mice to characterize the role of each LXR isoform during sperm maturation in the epididymis. We show here that although a certain redundancy exists in the functions of the two LXR isoforms, some physiological processes are more under the influence of only one of them. In both cases, aging males showed slight subfertility, associated with dyslipidemia, emphasizing the importance of lipid metabolism in relation with male fertility., (© 2016 by the Society for the Study of Reproduction, Inc.)

Published
2016
Full Text
View/download PDF

33. Epididymis response partly compensates for spermatozoa oxidative defects in snGPx4 and GPx5 double mutant mice.

Author

Noblanc A, Peltier M, Damon-Soubeyrand C, Kerchkove N, Chabory E, Vernet P, Saez F, Cadet R, Janny L, Pons-Rejraji H, Conrad M, Drevet JR, and Kocer A

Subjects
Animals, Male, Mice, Mice, Mutant Strains, Mice, Transgenic, Phospholipid Hydroperoxide Glutathione Peroxidase, Spermatozoa metabolism, Epididymis physiology, Glutathione Peroxidase genetics, Oxidative Stress, Spermatozoa physiology
Abstract

We report here that spermatozoa of mice lacking both the sperm nucleus glutathione peroxidase 4 (snGPx4) and the epididymal glutathione peroxidase 5 (GPx5) activities display sperm nucleus structural abnormalities including delayed and defective nuclear compaction, nuclear instability and DNA damage. We show that to counteract the GPx activity losses, the epididymis of the double KO animals mounted an antioxydant response resulting in a strong increase in the global H(2)O(2)-scavenger activity especially in the cauda epididymis. Quantitative RT-PCR data show that together with the up-regulation of epididymal scavengers (of the thioredoxin/peroxiredoxin system as well as glutathione-S-transferases) the epididymis of double mutant animals increased the expression of several disulfide isomerases in an attempt to recover normal disulfide-bridging activity. Despite these compensatory mechanisms cauda-stored spermatozoa of double mutant animals show high levels of DNA oxidation, increased fragmentation and greater susceptibility to nuclear decondensation. Nevertheless, the enzymatic epididymal salvage response is sufficient to maintain full fertility of double KO males whatever their age, crossed with young WT female mice.

Published
2012
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results