1. Temperature dependence of lipogenesis in isolated hepatocytes from rainbow trout (Salmo gairdneri).
- Author
-
Voss B and Jankowsky HD
- Subjects
- Acclimatization, Animals, Carbon Radioisotopes, In Vitro Techniques, Kinetics, Lactates metabolism, NAD metabolism, NADP metabolism, Oxidation-Reduction, Temperature, Tritium, Trout, Lipids biosynthesis, Liver metabolism
- Abstract
Temperature dependence of lipogenesis in trout liver cells was investigated in the presence of 5 mM lactate using either [14C]lactate or [3H]water. A ratio of 3H/14C-incorporation greater than one is found, irrespective of temperature. Acclimation of fish to 4, 10 or 16 degrees C affects neither the height of lipid synthesis nor its temperature sensitivity. The distribution of [14C]lactate between the main lipid classes and the capacities for cholesterol- and triacylglycerol-synthesis are correlated to the glycogen stores of the hepatocytes. A comparison of fatty acid synthesis and cholesterogenesis in livers of normal fed rat and of trout suggests a capability for lipogenesis in trout somewhat similar to that in mammals.
- Published
- 1986
- Full Text
- View/download PDF