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Made available in DSpace on 2022-08-22T19:24:10Z (GMT). No. of bitstreams: 1 RSD-2022-Agronomic-performance-and-chemical-composition-of-genotypes-and-cultivars-of-Megathyrsus-maximus-in-Roraimas-savannas-1.pdf: 109091 bytes, checksum: 1450c4c0f1861c4df1e00af41c9a3ff0 (MD5) Previous issue date: 2022

The effects of defoliation frequency (28, 35, 42 and 49 days) and defoliation intensity (30, 40 and 50 cm above the ground) on green dry matter (GDM) yield, and chemical composition of Panicum maximum cv. Mombaça were evaluated under natural field conditions at the Roraima ́s savannas. Defoliation regimes affect productivity and chemical composition of grass forage. The decrease in the pasture defoliation frequency and intensity improved the accumulation of forage, however it reduces the tissue concentrations of N, P, Ca, Mg and K. Irrespective of defoliation frequencies, the highest levels of N (23.99 g kg-1), P (2.12 g kg-1), Ca (4.22 g kg-1), Mg (2.87 g kg-1) and K (21.45 g kg-1) were recorded for the defoliation intensity at 40 cm above the ground. The use of defoliation frequency around 42 days and defoliation intensity of 42,9 cm above the ground can be considered adequate for the management of pastures of P. maximum cv. Mombaça, in order to provides higher forage productivity and quality, regrowth vigor, larger efficiency of forage utilization, greater tissue renewal and canopy structure more favorable to grazing. Made available in DSpace on 2021-08-04T14:04:04Z (GMT). No. of bitstreams: 1 RSD-2021-Forage-productivity-and-chemical-composition-of-Panicum-maximum-cv.-Mombaca-under-defoliations-intensities-and-frequencies.pdf: 197529 bytes, checksum: fa818da930760d565c0bc3dbda549b22 (MD5) Previous issue date: 2021

In this work were avaluated the effects of pasture rest periods on forage accumulation, chemical composition and morphogenic and structural characteristics of Megathyrsus maximus cv. Tamani in the Roraima´s savannas. Made available in DSpace on 2020-05-27T04:39:55Z (GMT). No. of bitstreams: 1 PubVet-2020-Desempenho-produtivo-composicao-quimica-RR.pdf: 426064 bytes, checksum: 17c74239ac356d03c8f6ed72d38db7b0 (MD5) Previous issue date: 2020 Título em Português: Desempenho produtivo, composição química e morfogênese de Megathyrsus maximus cv. Tamani sob períodos de descanso.

Made available in DSpace on 2020-12-10T09:09:02Z (GMT). No. of bitstreams: 1 1-Pasto-Certo-version-2.0-An-application-about-Brazilian-tropical-forage-cultivars-for-mobile-and-desktop-devices.pdf: 863590 bytes, checksum: ae5d39cfb70a7b726bbdad6e5a85dbc0 (MD5) Previous issue date: 2020

Made available in DSpace on 2020-12-12T22:28:01Z (GMT). No. of bitstreams: 1 5-High-Resolution-Linkage-Map-With-Allele.pdf: 1662161 bytes, checksum: f170934230a975599b17caabc555d7ed (MD5) Previous issue date: 2020

Made available in DSpace on 2020-04-29T04:38:28Z (GMT). No. of bitstreams: 1 RDS-2020-Caracteristicas-morfogenicas-e-estruturais-de-Megathyrsus-maximus-cv.-Centenario-sob-intensidades-de-desfolhacao1.pdf: 415463 bytes, checksum: 80253f1b9068111fc07abe6540190066 (MD5) Previous issue date: 2020

Made available in DSpace on 2019-03-19T00:47:39Z (GMT). No. of bitstreams: 1 PubVet2019AcumulodeforragemcomposicaoquimicaemorfogenesedeMegathyrsusmaximuscv.Queniasobdesfolhacaointermitente1.pdf: 452767 bytes, checksum: 62c06941f22cefa624b9f507bfcbca78 (MD5) Previous issue date: 2019

Genomic selection is an efficient approach to get shorter breeding cycles in recurrent selection programs and greater genetic gains with selection of superior individuals. Despite advances in genotyping techniques, genetic studies for polyploid species have been limited to a rough approximation of studies in diploid species. The major challenge is to distinguish the different types of heterozygotes present in polyploid populations. In this work, we evaluated different genomic prediction models applied to a recurrent selection population of 530 genotypes of Panicum maximum, an autotetraploid forage grass. We ,also investigated the effect of the allele dosage in the prediction, i.e., considering tetraploid (GS-TD) or diploid (GS-DD) allele dosage. A longitudinal linear mixed model was fitted for each one of the six phenotypic traits, considering different covariance matrices for genetic and residual effects. A total of 41,424 genotypingby- sequencing markers were obtained using 96-plex and Pst1 restriction enzyme, and quantitative genotype calling was performed. Six predictive models were generalized to tetraploid species and predictive ability was estimated by a replicated fivefold cross-validation process. GS-TD and GS-DD models were performed considering 1,223 informative markers. Overall, GS-TD data yielded higher predictive abilities than with GS-DD data. However, different predictive models had similar predictive ability performance. In this work, we provide bioinformatic and modeling guidelines to consider tetraploid dosage and observed that genomic selection may lead to additional gains in recurrent selection program of P. maximum. Made available in DSpace on 2019-12-31T00:38:25Z (GMT). No. of bitstreams: 1 GenomicSelectionwithAlleleDosage.pdf: 1504705 bytes, checksum: 070a9fc1afa0161e4c6ea7502c5f52f8 (MD5) Previous issue date: 2019

Made available in DSpace on 2019-06-26T00:40:18Z (GMT). No. of bitstreams: 1 2019RDSRespostadepastagensdeMegathyrsusmaximuscv.Zuriafrequenciadedesfolhacao.pdf: 222339 bytes, checksum: 6b2b471330f22f7a596aacd5b04beaf8 (MD5) Previous issue date: 2019

Made available in DSpace on 2019-03-19T00:48:03Z (GMT). No. of bitstreams: 1 PubVet2019ProdutividadedeforragemecaracteristicasmorfogenicaseestruturaisdeMegathyrsusmaximuscv.Zurisobniveisdedesfolhacao.pdf: 358889 bytes, checksum: e3bf6c8bcd3e4153f22e8c92a98389bd (MD5) Previous issue date: 2019

In experiments with selection of perennial plants, the initial performance is expected to be maintained throughout the productive life. The probability of repeating the agronomic response can be evaluated by the repeatability coefficient. The objective was to estimate repeatability coefficients of agronomic characteristics in Panicum maximum Jacq. using different methods and identify the best combinations between harvests according to the genotypic stabilization. Seventeen genotypes were evaluated in a randomized complete block design with three replicates. The coefficients of repeatability were estimated by the methods: ANOVA, structural analysis based on the mean of the correlation coefficients (SACOR), analysis of principal components based on the covariance matrix (PCCOV) and correlation matrix (PCCOR). In the genotypic stabilization, coefficients estimated by ANOVA and PCCOR were used. For total DM, repeatability coefficients ranged from 0.3500 to 0.4300 by the ANOVA and PCCOR methods, respectively. High repeatability coefficients were found for the characteristic leaf DM, while low coefficients were observed for leaf and stem percentages and leaf:stem ratio. For the genotypic stabilization of total DM and leaf:stem ratio, the best coefficients were observed for the combination of harvests 5 to 8. There are variations in the estimate of repeatability by different methods and the highest coefficients were those of the principal components based on the correlation and covariance matrices. For the genotypic stabilization, the best coefficients of repeatability and determination were observed for the harvests performed in the second rainy period. Made available in DSpace on 2019-12-31T00:38:51Z (GMT). No. of bitstreams: 1 Repeatabilityandgenotypicstability.pdf: 375500 bytes, checksum: ebdf11750194b8c14759b604db7f7927 (MD5) Previous issue date: 2019

Made available in DSpace on 2018-05-16T00:47:46Z (GMT). No. of bitstreams: 1 PubVet2018RendimentodeforragemcomposicaoquimicaemorfogenesedeMegathyrsusmaximuscv.Tanzania1sobfrequenciasdedesfolhacao.pdf: 455112 bytes, checksum: cb4bd5dcb30c5dfd3358d2717567d63c (MD5) Previous issue date: 2018-05-15

Ainda há muitas cultivares por vir, cada uma com vantagens específicas. A tendência é que sejam liberadas espécies menos generalistas, sou seja, adaptadas às demandas específicas do setor produtivo.

This study aimed to isolate and identify endophytic fungi from the forage grass P. maximum and evaluate their ability to inhibit the growth of plant pathogenic fungi. One sample from P. purpureum grass was also included. Surface disinfected stem fragments were used for endophytic fungal isolation. One hundred and twenty-six endophytic fungi were isolated, of which 118 were from P. maximum and eight from P. purpureum. Morphological characteristics and internal transcribed spacer (ITS) and 18S (NS) sequence comparisons identified most isolated endophytic fungi as belonging to the phylum Ascomycota, with Sarocladium being the dominant genus. The isolates were subjected to in vitro antagonism tests against pathogenic fungi, and 31 endophytic fungi inhibited the growth of Bipolaris maydis, Penicillium expansum, and Sclerotinia minor. The results expand our knowledge of the diversity of endophytes associated with tropical grasses and suggest that they may represent new sources of antifungal metabolites for biocontrol and biotechnological purposes. Made available in DSpace on 2019-07-05T00:52:54Z (GMT). No. of bitstreams: 1 FungalendophytesofPanicummaximum.pdf: 308948 bytes, checksum: 906943033eabb016539b917e21260361 (MD5) Previous issue date: 2018

O efeito de intensidades de desfolhação (20, 30, 40 e 50 cm acima do solo) sobre a produção de forragem e características morfogênicas e estruturais de Megathyrsus maximus cv. Tanzânia-1 foi avaliado nos cerrados de Roraima. Made available in DSpace on 2018-06-07T01:07:52Z (GMT). No. of bitstreams: 1 PUBVETv12Caracteristicas.pdf: 496557 bytes, checksum: c5e604a57937d59cd386f376df9f92c8 (MD5) Previous issue date: 2018-06-06

Made available in DSpace on 2017-09-27T10:31:19Z (GMT). No. of bitstreams: 1 PubVet2017ProdutividadedeforragemcomposicaoquimicaemorfogenesedeMegathyrsusmaximuscv.Mombacasobperiodosdedescanso.pdf: 327288 bytes, checksum: 5adcd5df5ee678f658ac86c17a047186 (MD5) Previous issue date: 2017-09-26

Made available in DSpace on 2017-09-27T10:30:59Z (GMT). No. of bitstreams: 1 PubVet2017AcumulodeforragememorfogenesedeMegathyrsusmaximuscv.Mombacasobniveisdefosforo1.pdf: 381179 bytes, checksum: dbdf8b04f09c627d5ffebf8378eb7c66 (MD5) Previous issue date: 2017-09-26

Selection of superior forage genotypes is based on agronomic traits assayed in repeated measures. The questions are how repeatable the performance of individual genotypes is and how many harvests are needed to select the best genotypes. The objectives were to estimate repeatability coefficients of dry matter yield (DMY) and forage quality, their phenotypic stability and the number of harvests needed for an accurate selection. Two randomized complete block design experiments data with 24 genotypes each, undergoing 12 and 16 harvests, over a period of 2 and 3 years, respectively, were used. The DMY repeatability estimates ranged from 0.42 to 0.55, suggesting a low heritability. The mean numbers of repeated measures were 5 and 7 harvests for 0.80 and 0.85 accuracy, respectively. The inclusion of the first two harvests negatively affects the estimates. Repeatability for quality traits ranged from 0.30 to 0.69, indicating low to moderate heritability. Made available in DSpace on 2017-10-14T10:33:19Z (GMT). No. of bitstreams: 1 RepeatabilitynumberofharvestsandphenotypicstabilityofdrymatteryieldandqualitytraitsofPanicummaximumjacq.pdf: 157585 bytes, checksum: 750e23dd26d49456c87b8fcf5956887b (MD5) Previous issue date: 2017-10-13 Título em português: Repetibilidade, número de colheitas e estabilidade fenotípica da produção de matéria seca e de características de qualidade de Panicum maximum jacq.

Made available in DSpace on 2019-03-23T00:42:39Z (GMT). No. of bitstreams: 1 84139671PB.pdf: 1217108 bytes, checksum: 7d4aae8e8bf35fb6c64c9db451f605f4 (MD5) Previous issue date: 2016

Objetivou-se com este trabalho avaliar a diversidade genética e a importância relativa de caracteres morfológicos para o estudo da diversidade genética em genótipos de Stylosanthes capitata com base em caracteres morfológicos. Foram avaliadas 10 famílias de polinização aberta (297-2, 49-5, 1064-4, 49-4, 1095-5, 141-6, 1064-6, 297-6, 625-3, 111-3), previamente selecionadas pelo programa de melhoramento da Embrapa Gado de Corte e a cultivar Campo Grande como testemunha. O delineamento experimental foi inteiramente casualizado, com quatro repetições. Foram avaliadas as características morfológicas: forma de crescimento, comprimento, largura, relação comprimento:largura dos folíolos centrais e laterais, altura da planta, comprimento da haste e número de ramificações secundárias. A matriz de distância generalizada do Mahalanobis foi obtida utilizando-se as médias fenotípicas para cada característica e a matriz de variâncias e covariâncias residuais. Em posse da matriz de dissimilaridade, as famílias e a cultivar Campo Grande foram agrupadas utilizando o método de Ward. A análise de variáveis canônicas (VC) também foi realizada utilizando-se a média fenotípica dos caracteres avaliados e a matriz de variâncias e covariâncias residuais. Por meio da análise de agrupamento pelo método de Ward foi possível reunir os 11 genótipos em cinco grupos. A análise de variáveis canônicas foi realizada com grande eficiência, pois permitiu resumir a variação total observada nos nove caracteres morfológicos em apenas três VC, que acumularam 81,65% da variação total dos dados. Também foi possível realizar o estudo da importância das características para o agrupamento. Os caracteres morfológicos largura do folíolo central, relação comprimento largura dos folíolos central e lateral, largura do folíolo lateral e ramificações secundárias figuram entre os de maior importância para a avaliação da diversidade genética entre genótipos de Stylosanthes capitata. Made available in DSpace on 2018-02-24T00:38:04Z (GMT). No. of bitstreams: 1 57DiversidadegeneticaemacessosdeStylosanthescapitata2015.pdf: 274562 bytes, checksum: 09af0142501aa816d7d92c3f5714cea5 (MD5) Previous issue date: 2018-02-23

The objective of this study was to evaluate the genotypic stability of agronomic traits in hybrids of Panicum maximum. Hybrids originating from the crosses between two sexual parents and cultivars Mombasa and Tanzania were evaluated in an incomplete-block design. Evaluated traits were total dry matter, leaf dry matter, stem dry matter, percentage of leaves, and leaf:stem ratio. These traits were evaluated in six harvests. Overall, higher repeatability was observed for the traits total dry matter and leaf dry matter, especially when harvests 4, 5, and 6, occurring in spring and summer, were evaluated. The leaf dry matter was the trait that provided the greatest repeatability and determination. The repeatability of stem dry matter, percentage of leaves, and leaf:stem ratio had a low magnitude, even when the coefficient was estimated based on the harvests of better stabilization for the other variables. The hybrids achieved genotypic stabilization in the harvests made in the second rainy season. Harvests made in the rainy season provide greater repeatability and determination, and the inclusion of the dry-season harvest is detrimental to the process of selection of low-repeatability traits such as percentage of leaves. Made available in DSpace on 2018-02-23T00:32:09Z (GMT). No. of bitstreams: 1 48GenotypicstabilizationofagronomictraitsinPanicummaximumJacq.hybrids2015.pdf: 726903 bytes, checksum: 1c995621037287f847ae6795baa494d1 (MD5) Previous issue date: 2018-02-22

O experimento foi conduzido objetivando-se determinar os coeficientes de repetibilidade, por quatro métodos (Análise de Variância, de Componentes prin-cipais - matriz de correlação e covariância e a Análise estrutural), o número de cortes necessários para aumento de acurácia e a estabilização fenotípica de características de produção em acessos de Panicum maximum. O experimento foi conduzido em delineamento inteiramente casualizado com 35 genótipos e três repetições. Foram realizados cinco cortes e as amostras foram pesadas e secas para obtenção da produção de matéria seca total, de colmo, folhas e material morto. Os coeficientes de repetibilidade nos diferentes métodos, para todas as características, oscilaram entre 0,1867 e 0,6583. As estimativas da repetibilidade obtidas pelo método da análise de variância foram quase sempre menores que as obtidas pelos demais métodos. Pelo método dos componen-tes principais (baseado na matriz de covariância), as estimativas foram sempre maiores em relação aos demais métodos. Portanto, é possível recomendar esse método para estimativas do coeficiente de repetibilidade para as características avaliadas nesse estudo. Considerando satisfatórios os níveis de 80 ou 90% de confiabilidade para avaliação da superioridade relativa dos acessos para todas as características avaliadas, as cinco medições realizadas são suficientes para escolha do melhor acesso.

Soil fertility of the Brazilian savanna (Cerrado) is natu-rally poor. Extensive areas of pastures located in the central part of the territory are cultivated with Brachiaria grasses, which are less demanding for soil nutrients and lime than other species (Rao et al. 1998). On the other hand, Panicum maximum cultivars, such as the high yielding Mombaça grass recommended for intensive beef and dairy cattle systems (Euclides et al. 2008), must be seeded with a higher amount of fertilizer, especially phosphorus (P). Consequently, an effort is underway to select P. maximum genotypes with low P demand and high responsiveness. The objective of this study was to evaluate dry matter yields of genotypes of P. maximum in response to doses of P and lime in the Brazilian Cerrado. Made available in DSpace on 2018-12-14T00:00:15Z (GMT). No. of bitstreams: 1 44DrymatteryieldofpromisingPanicummaximumgenotypesinresponsetophosphorusandlimingonBraziliansavannah2013.pdf: 327728 bytes, checksum: 22b9adda626c4c02b17769afe08831fa (MD5) Previous issue date: 2018-02-22

Made available in DSpace on 2020-04-24T14:32:55Z (GMT). No. of bitstreams: 1 v71n1a03-SA-2013-0102.indd-Scientia-agricola-v71n1a03.pdf: 4102879 bytes, checksum: 3ccf96841dfbdb4858131169a3843d04 (MD5) Previous issue date: 2014

Guinea grass (Panicum maximum Jacq.) is a tropical African grass often used to feed beef cattle, which is an important economic activity in Brazil. Brazil is the leader in global meat exportation because of its exclusively pasture-raised bovine herds. Guinea grass also has potential uses in bioenergy production due to its elevated biomass generation through the C4 photosynthesis pathway. We generated approximately 13 Gb of data from Illumina sequencing of P. maximum leaves. Four different genotypes were sequenced, and the combined reads were assembled de novo into 38,192 unigenes and annotated; approximately 63% of the unigenes had homology to other proteins in the NCBI non-redundant protein database. Functional classification through COG (Clusters of Orthologous Groups), GO (Gene Ontology) and KEGG (Kyoto Encyclopedia of Genes and Genomes) analyses showed that the unigenes from Guinea grass leaves are involved in a wide range of biological processes and metabolic pathways, including C4 photosynthesis and lignocellulose generation, which are important for cattle grazing and bioenergy production. The most abundant transcripts were involved in carbon fixation, photosynthesis, RNA translation and heavy metal cellular homeostasis. Finally, we identified a number of potential molecular markers, including 5,035 microsatellites (SSRs) and 346,456 single nucleotide polymorphisms (SNPs). To the best of our knowledge, this is the first study to characterize the complete leaf transcriptome of P. maximum using highthroughput sequencing. The biological information provided here will aid in gene expression studies and marker-assisted selection-based breeding research in tropical grasses.

This experiment was carried out with the objectives of determining repeatability coefficients, the number of harvests necessary to increase accuracy and the genotypic stability of yield characteristics in Brachiaria brizantha cultivars in the northeast region of Brazil in the period from July 2009 to March 2011. The experimental design used was completely randomized with three treatments (Brachiaria brizantha var. Marandu, Xaraés and Piatã) and three replications. Each plot was harvested seven times: five in the rainy season and two in the dry season. Immediately after each harvest, the forage was weighed and dried to obtain total, stem, leaf and dead dry matter yields. For the estimation of the repeatability coefficient, analysis of variance, principal components (correlation and covariance matrices) and structural analyses were used. The repeatability coefficients (r) obtained by the different methods, for all characteristics evaluated, varied from 0.009 to 0.837. The estimates of the repeatability coefficients obtained for all characteristics evaluated by the method of analyses of variance were almost always lower than those obtained by the other methods. By the principal components method (based on the covariance matrix), the estimates were always higher than those from other methods. Considering that levels of 80 or 90% confidence are satisfactory in the evaluation of the relative superiority of the cultivars for all the evaluated characteristics, with the exception of dead matter yield, the seven harvests are sufficient for the choice of the best cultivar to be used in the northeast region of Brazil. Made available in DSpace on 2018-02-23T00:31:44Z (GMT). No. of bitstreams: 1 46RepeatabilityofagronomiccharactersinBrachiariabrizanthacultivars2013.pdf: 322049 bytes, checksum: 2a2969945ae1898faeae823b4f673faa (MD5) Previous issue date: 2018-02-22

The Panicum maximum breeding program coordinated by the Brazilian Agricultural Research Corporation (Embrapa) has been evaluating and selecting genotypes under different soil and climatic conditions, with the objective to release new cultivars adapted to the diverse regions of Brazil. For the Amazon biome, small-plot experiments carried out in Acre between 2003 and 2005 allowed the selection of some promising genotypes (Valentim and Andrade 2005; Valentim et al. 2006; Andrade and Valentim 2009), with higher potential for forage growth than cultivars on the market. The objec-tive of this work was to compare 2 new P. maximum genotypes with cv. Tanzânia in relation to carrying ca-pacity and animal performance, when managed under rotational stocking in the Amazon biome. Publicado também nos Anais do 22º INTERNATIONAL GRASSLAND CONGRESS, Sidney, 2013.

Brazil has the largest commercial beef cattle herd and is the main beef exporter in the world. Cultivated pastures are the basis for the Brazilian beef production, and occupy an area of 101.4 million hectares. However, very few forage cultivars are commercially available, and the majority of these are of apomictic reproduction, thus genetically homogeneous. Tropical forage breeding is at its infancy, but much investment and efforts have been applied in the last three decades and some new cultivars have been released. In this paper, origin of different species, modes of reproduction, breeding programs and targets are discussed and the resulting new cultivars released are presented. O Brasil tem o maior rebanho bovino comercial e é o principal exportador de carne do mundo. Pastagens cultivadas são a base para a produção de carne brasileira, e ocupam uma área de 101,4 milhões de hectares. Entretanto, poucas cultivares de forrageiras estão disponíveis comercialmente, e a maioria delas são de reprodução por apomixia, portanto, geneticamente homogêneas. O melhoramento de forrageiras tropicais ainda está na sua infância, porém muitos esforços e investimentos foram despendidos nas últimas três décadas e algumas novas cultivares foram lançadas. Neste trabalho são apresentados e discutidos a origem das diferentes espécies, o modo reprodutivo, os programas de melhoramento e as novas cultivares resultantes lançadas.

O objetivo deste trabalho foi avaliar as características anatômicas e morfofisiológicas de lâminas foliares de genótipos de Panicum maximum e determinar quais as mais relevantes para a discriminação precoce do valor nutritivo de genótipos promissores. O experimento foi realizado entre janeiro de 2006 e novembro de 2007, em Dourados, MS. Foram avaliados 23 genótipos de P. maximum pré-selecionados do programa de melhoramento da Embrapa Gado de Corte. Utilizou-se o delineamento experimental de blocos ao acaso, com três repetições. Foram avaliadas 27 características anatômicas e morfofisiológicas de lâminas foliares em cada genótipo. Para cada característica estudada, os resultados foram agrupados pelo teste de Scott‑Knott. A importância relativa das características avaliadas para a avaliação da divergência genética foi determinada pelo método de Singh. As características que melhor discriminaram os genótipos foram as proporções ? na seção transversal das lâminas ? das epidermes adaxial, abaxial, adaxial + abaxial, do mesofilo e do tecido vascular + esclerênquima. A área foliar específica, a área foliar e o comprimento de lâmina foram as características morfofisiológicas que mais contribuíram para a discriminação dos genótipos; porém, sua importância relativa foi muito menor que a das características anatômicas. A proporção ocupada pela bainha parenquimática dos feixes e a largura das lâminas apresentam instabilidade fenotípica.

The Guineagrass (Panicum maximum Jacq.) is one of the most important tropical forage grasses, but genetic knowledge and tools regarding this species are still limited. Therefore, 20 novel polymorphic microsatellite markers were developed, validated, and employed in estimating genetic relationships among 25 P. maximum genotypes selected from a Brazilian germplasm collection. In addition, they were tested for cross-species amplification in four other forage grass species. The number of alleles observed for each locus ranged from 4 to 12 (average 6.7). The values of polymorphism information content (PIC) varied from 0.41 to 0.83 (average 0.61) and the discriminating power (D) ranged from 0.53 to 0.98 (average 0.72). Cross-amplification demonstrated the potential transferability of these microsatellites to four tropical forage grass species. Cluster analysis based on the unweighted pair group method revealed three distinct groups: two clusters consisted of P. maximum genotypes and a third cluster, consisted of the other tropical forage grass species. The data demonstrated that the microsatellites developed herein have potential for germplasm characterization and genetic diversity analysis in P. maximum and other forage grass species.