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BackgroundPregnancies complicated by Coronavirus Disease 2019 (COVID-19) are at an increased risk of severe morbidity due to physiologic changes in immunologic, cardiovascular, and respiratory function. There is little is known about how severity of COVID-19 changes protein and metabolite expression in pregnancy.ObjectiveThis study aims to investigate the pathophysiology behind various clinical trajectories in pregnant patients diagnosed with COVID-19 using multi-omics profiling.Study designThis is a prospective cohort study of 30 pregnant patients at a single tertiary care center. Participants were categorized by severity of COVID-19 disease (control, asymptomatic, mild/moderate, or severe). Maternal serum samples underwent LC-MS-based multiomics analysis for profiling of proteins, lipids, electrolytes, and metabolites. Linear regression models were used to assess how disease severity related to analyte levels. Reactome pathway enrichment analysis was conducted on differential analytes.ResultsOf 30 participants, 25 had confirmed diagnosis of COVID-19 (6 asymptomatic (one post-infection), 13 mild/moderate (all post-infection), 6 severe), and 5 participants were controls. Severe COVID-19 was associated with distinct profiles demonstrating significant proteomic and lipidomic signatures which were enriched for annotations related to complement and antibody activity. (FDR < 0.05). Downregulated analytes were not significantly enriched but consisted of annotation terms related to lipoprotein activity (FDR > 0.2). Post-infection mild/moderate COVID-19 did not have significantly altered serum protein, metabolite, or lipid metabolite levels compared to controls.ConclusionsPregnancies with severe COVID-19 demonstrate greater inflammation and complement activation and dysregulation of serum lipids. This altered multiomic expression provides insight into the pathophysiology of severe COVID-19 in pregnancy and may serve as potential indicators for adverse pregnancy outcomes.

Je-ju-jo-rit-dae is a medicinal plant widely used in oriental medicine. However, its high reproductive rate has become a problem for the environmental ecosystem in Jeju. The extracts of the je-ju-jo-rit-dae are known for their antioxidant and anti-cancer effects. Still, no research has been conducted on significant impact and efficacy as an external skin agent, which has yet to be used commercially. This study performed a highly anti-oxidizing analysis by extracting the handle with methanol as a non-skin material at Je-ju-jo-rit-dae and identified its anti-inflammatory function and collagen synthesis ability. The HPLC analysis of these functional divisions shows Asiatic acid and Asiaticoside among the high levels of flavonoids and triterpenoid. The efficiency of Je-ju-jo-rit-dae found in the study is likely a good material for high-functioning skin external agents. [ABSTRACT FROM AUTHOR]

Recipients of solid organ transplants who become pregnant represent an obstetrically high-risk population. Preconception planning and effective contraception tailored to the individual patient are critical in this group. Planned pregnancies improve both maternal and neonatal outcomes and provide a window of opportunity to mitigate risk and improve lifelong health. Optimal management of these pregnancies is not well defined. Common pregnancy complications after transplantation include hypertension, preterm birth, infection, and metabolic disease. Multidisciplinary preconception and prepartum management, and counseling decrease complications and benefit the maternal-neonatal dyad. [ABSTRACT FROM AUTHOR]