Search

Your search keyword '"Jamil, K M"' showing total 27 results
Start Over Author "Jamil, K M"
27 results on '"Jamil, K M"'

12. Salmonella enterica Serovar Typhi-Specific Immunoglobulin A Antibody Responses in Plasma and Antibody in Lymphocyte Supernatant Specimens in Bangladeshi Patients with Suspected Typhoid Fever

Author

Sheikh, Alaullah, primary, Bhuiyan, M. Saruar, additional, Khanam, Farhana, additional, Chowdhury, Fahima, additional, Saha, Amit, additional, Ahmed, Dilruba, additional, Jamil, K. M. A., additional, LaRocque, Regina C., additional, Harris, Jason B., additional, Ahmad, Mian Mashhud, additional, Charles, Richelle, additional, Brooks, W. Abdullah, additional, Calderwood, Stephen B., additional, Cravioto, Alejandro, additional, Ryan, Edward T., additional, and Qadri, Firdausi, additional

Published
2009
Full Text
View/download PDF

19. Salmonella entericaSerovar Typhi-Specific Immunoglobulin A Antibody Responses in Plasma and Antibody in Lymphocyte Supernatant Specimens in Bangladeshi Patients with Suspected Typhoid Fever

Author

Sheikh, Alaullah, Bhuiyan, M. Saruar, Khanam, Farhana, Chowdhury, Fahima, Saha, Amit, Ahmed, Dilruba, Jamil, K. M. A., LaRocque, Regina C., Harris, Jason B., Ahmad, Mian Mashhud, Charles, Richelle, Brooks, W. Abdullah, Calderwood, Stephen B., Cravioto, Alejandro, Ryan, Edward T., and Qadri, Firdausi

Abstract

ABSTRACTMany currently available diagnostic tests for typhoid fever lack sensitivity and/or specificity, especially in areas of the world where the disease is endemic. In order to identify a diagnostic test that better correlates with typhoid fever, we evaluated immune responses to Salmonella entericaserovar Typhi (serovar Typhi) in individuals with suspected typhoid fever in Dhaka, Bangladesh. We enrolled 112 individuals with suspected typhoid fever, cultured day 0 blood for serovar Typhi organisms, and performed Widal assays on days 0, 5, and 20. We harvested peripheral blood lymphocytes and analyzed antibody levels in supernatants collected on days 0, 5, and 20 (using an antibody-in-lymphocyte-supernatant [ALS] assay), as well as in plasma on these days. We measured ALS reactivity to a serovar Typhi membrane preparation (MP), a formalin-inactivated whole-cell preparation, and serovar Typhi lipopolysaccharide. We measured responses in healthy Bangladeshi, as well as in Bangladeshi febrile patients with confirmed dengue fever or leptospirosis. We categorized suspected typhoid fever individuals into different groups (groups I to V) based on blood culture results, Widal titer, and clinical features. Responses to MP antigen in the immunoglobulin A isotype were detectable at the time of presentation in the plasma of 81% of patients. The ALS assay, however, tested positive in all patients with documented or highly suspicious typhoid, suggesting that such a response could be the basis of improved diagnostic point-of-care-assay for serovar Typhi infection. It can be important for use in epidemiological studies, as well as in difficult cases involving fevers of unknown origin.

Published
2009
Full Text
View/download PDF

25. Distinct mechanisms of action of V1 antagonists OPC-21268 and [d(CH2)5Tyr(Me)AVP] in mesangial cells.

Author

Jamil KM, Watanabe T, Nakao A, Okuda T, and Kurokawa K

Subjects
Animals, Arginine Vasopressin pharmacology, Cells, Cultured, Deamino Arginine Vasopressin pharmacology, Dose-Response Relationship, Drug, Glomerular Mesangium drug effects, Kinetics, Rats, Receptors, Vasopressin metabolism, Antidiuretic Hormone Receptor Antagonists, Arginine Vasopressin metabolism, Calcium metabolism, Glomerular Mesangium metabolism, Piperidines pharmacology, Quinolones pharmacology
Abstract

The effects of two arginine vasopressin (AVP) V1 receptor antagonists, a peptide analogue [d(CH2)5Tyr(Me)AVP] and an orally active non-peptide OPC-21268, on AVP-stimulated Ca transients and 3H-AVP binding were examined in cultured rat mesangial cells. Although both [d(CH2)5Tyr(Me)AVP] and OPC-21268 dose-dependently inhibited AVP-stimulated Ca transients and 3H-AVP binding, their effects were quite different. Magnitude of inhibition of Ca transients by OPC-21268 was comparable when OPC-21268 was added together with or 5 min prior to AVP. However, the inhibition by [d(CH2)5Tyr(Me)AVP] was greater when it was added prior to AVP than when it was added together with AVP. Moreover, washing mesangial cells after OPC-21268 treatment, but not after [d(CH2)5Tyr(Me)AVP] treatment, completely restored AVP-stimulated Ca transients to the control level without antagonists. These results indicate that the cellular mechanisms for two V1 receptor antagonists are different: while the effect of OPC-21268 as a vasopressin antagonist appears to be competitive and reversible, the effect of the peptide antagonist [d(CH2)5Tyr(Me)AVP] seems to be rather irreversible in nature.

Published
1993
Full Text
View/download PDF

26. Expression of platelet activating factor receptor in renal tubular cell line (LLC-PK1).

Author

Jamil KM, Takano T, Nakao A, Honda Z, Shimizu T, Watanabe T, and Kurokawa K

Subjects
Animals, Blotting, Northern, Calcium metabolism, Cell Line, Cloning, Molecular, DNA Probes, Dogs, Guinea Pigs, Mice, Platelet Activating Factor pharmacology, RNA, Messenger metabolism, Gene Expression, Kidney Tubules metabolism, Platelet Membrane Glycoproteins, Receptors, Cell Surface genetics, Receptors, G-Protein-Coupled
Abstract

Northern blot analyses of poly(A)+ RNA extracted from cloned renal tubule cells, LLC-PK1, MDCK and JTC-12 cells, were performed using an isolated cDNA of guinea pig platelet activating factor (PAF) receptor as a probe. The results showed the expression of single entity of PAF receptor mRNA in LLC-PK1, but not in MDCK or JTC-12 cells. PAF also induced a dose-dependent elevation of intracellular calcium in LLC-PK1 cells as assessed by Fura-2 fluorescence signals, which was completely blocked by a PAF receptor antagonist, WEB 2170. These results indicate a PAF receptor-effector system in a renal tubular cell line LLC-PK1, providing a possible model system for studying physiological roles of PAF receptors in renal tubules.

Published
1992
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results