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The textile industry needs to adopt environmentally sustainable approaches to address ecologically damaging practices. Whilst driven by initiatives such as Textiles 2030, it is current students who will carry this agenda forward. This project investigated pedagogical approaches to develop sustainable textiles for the fashion design curriculum. Pilot studies, using bacterial cellulose (BC) as a material for millinery, revealed members of the public were prepared to experiment with this novel material, and BC was compatible with traditional hat-making techniques. A further study challenged secondary school students, based on an experiential learning model, to grow their own BC biofilm, exploring this as a sustainable apparel fabric. Initial attitudes of reluctance developed into acceptance once engaged in the practical activity. This study illustrates that with appropriate communication and education strategies, the principles of sustainability in fashion, and the acceptability of novel materials, can be engendered in different audiences.

The health benefits of physical activity are well documented. Patients with hypertrophic cardiomyopathy (HCM) are often discouraged from participating in physical activity due to a perceived increase in the risk of sudden cardiac death (SCD). As a result, only 45% of patients with HCM meet the minimum guidelines for physical activity, and many report an intentional reduction in exercise following diagnosis. Despite most SCD being unrelated to HCM, guidelines traditionally focused on the avoidance of potential risk through restriction of exercise, without clear recommendations on how to negate the negative health impact of inactivity. Retrospective reviews have demonstrated that the majority of cardiac arrests in patients with HCM occurred at rest or on mild exertion and that the overall incidence of HCM-related SCD is significantly lower than previously reported. We will discuss current international guidelines and recommendations and consider the outcomes of various studies that have investigated the effects of exercise of different intensities on patients with HCM. In light of the growing evidence suggesting that carefully guided exercise can be both beneficial and safe in patients with HCM, we ask whether it is time to let the shackles off exercise restriction in HCM.

Bacterial cellulose (BC) has been suggested as a sustainable alternative textile for apparel. Previous studies have evaluated the production of BC sheets and the suitability of these to form garment shapes. The laboratory measured physical performance characteristics of BC from an apparel perspective remain relatively unexplored. The aim of this study was to produce reproducible sheets of BC, enabling the evaluation of the performance of the BC in an apparel textile testing context, and comparison to other textile materials. Grown in sterile black tea with glucose, the BC presented as a mesh of non-woven nanofibers, and thus comparison was made with three non-woven fabrics. It has also been suggested that BC could be used as ‘vegetable’ leather; therefore, performance comparisons were conducted with animal skins. Utilizing British, European and International standard test methods, the selected fabrics were evaluated for their performance in tensile, elongation, moisture vapor permeability and abrasion tests, relevant for an apparel end-use. Tensile strength testing revealed that BC is weaker than its animal counterparts but does display similar physical characteristics at the point of failure; however, it displayed a higher tensile strength than the non-woven fabrics chosen for comparison. BC was the least breathable and most moisture-retentive of all the fabrics tested, raising questions regarding its suitability and comfort for apparel applications in its untreated state. However, BC displayed superior performance when tested for resistance to abrasion, suggesting it could be best utilized in the form of encapsulated patches in items subjected to this type of damage.

During the coronavirus pandemic, second-year students on the B.Sc. molecular biology and genetics degree at Istanbul Technical University sat an open-ended online exam for a microbiology course in which one of the compulsory questions asked how the course had helped them during the first phase of the pandemic (April–July 2020). Fifty of 69 students gave consent for their (anonymous) responses to be analysed in order to discern any key ways in which their knowledge had been applied. The aim of the study was to investigate whether taking an advanced microbiology course increases understanding of the Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) pandemic and has a positive impact on student behaviours with respect to public health practices. Findings were divided into four major themes: course content (information), application of course content to behavioural change (practice), professionalism and their ‘audience’ whilst at home in lockdown (family and friends). Social distancing, wearing face masks, and hand and surface hygiene were described as important behaviours, with this practice informed by their basic microbiology knowledge. This paper describes a scenario where rote assessment can be used to assess wider scientific literacy with respect to application in society, providing students with an opportunity to incorporate and apply their learning into real-life situations, whilst tutors can assess constructivist learning, conceptual understanding and impact on student behaviour., The microbiology examination assignment at Istanbul Technical University during the coronavirus pandemic is an excellent example of assessing wider scientific literacy with respect to application in society.

Phage therapy recently passed a key milestone with success of the first regulated clinical trial using systemic administration. In this single-arm non-comparative safety study, phages were administered intravenously to patients with invasive Staphylococcus aureus infections with no adverse reactions reported. Here, we examined features of 78 lytic S. aureus phages, most of which were propagated using a S. carnosus host modified to be broadly susceptible to staphylococcal phage infection. Use of this host eliminates the threat of contamination with staphylococcal prophage — the main vector of S. aureus horizontal gene transfer. We determined the host range of these phages against an international collection of 185 S. aureus isolates with 56 different multilocus sequence types that included multiple representatives of all epidemic MRSA and MSSA clonal complexes. Forty of our 78 phages were able to infect > 90% of study isolates, 15 were able to infect > 95%, and two could infect all 184 clinical isolates, but not a phage-resistant mutant generated in a previous study. We selected the 10 phages with the widest host range for in vitro characterization by planktonic culture time-kill analysis against four isolates:- modified S. carnosus strain TM300H, methicillin-sensitive isolates D329 and 15981, and MRSA isolate 252. Six of these 10 phages were able to rapidly kill, reducing cell numbers of at least three isolates. The four best-performing phages, in this assay, were further shown to be highly effective in reducing 48 h biofilms on polystyrene formed by eight ST22 and eight ST36 MRSA isolates. Genomes of 22 of the widest host-range phages showed they belonged to the Twortvirinae subfamily of the order Caudovirales in three main groups corresponding to Silviavirus, and two distinct groups of Kayvirus. These genomes assembled as single-linear dsDNAs with an average length of 140 kb and a GC content of c. 30%. Phages that could infect > 96% of S. aureus isolates were found in all three groups, and these have great potential as therapeutic candidates if, in future studies, they can be formulated to maximize their efficacy and eliminate emergence of phage resistance by using appropriate combinations.

Aims/Background Heart failure affects approximately 1 million people in the UK, adversely affecting quality of life, functional capacity and cognitive health. Iron deficiency complicates heart failure in approximately 50% of patients. Giving intravenous ferric carboxymaltose has been shown to improve quality of life in patients with heart failure (New York Heart Association class and Kansas City Cardiomyopathy Questionnaire). Methods A quality improvement project was designed to assess the feasibility, safety and cost implications of establishing an intravenous iron service in the authors' centre. Results Between July and December 2019 61 patients who were screened met the inclusion criteria and were administered intravenous ferric carboxymaltose. There were statistically significant improvements in ferritin levels (83.3 ug/litre to 433 ug/litre; PConclusions Intravenous iron replacement with ferric carboxymaltose is safe and cost effective, and should be considered in eligible iron-deficient patients with symptomatic heart failure. Integration with another day case intravenous service represented the most logistically simple and economically viable method of service delivery.

© 2020 by the authors. Licensee MDPI, Basel, Switzerland. There are few peer-reviewed publications about public engagement with science that are written by microbiologists; those that exist tend to be a narrative of an event rather than a hypothesis-driven investigation. However, it is relatively easy for experienced scientists to use a scientific method in their approach to public engagement. This short communication describes three public engagement activities hosted by the authors, focused on biofilm control: hand hygiene, plaque control and an externally applied antimicrobial coating. In each case, audience engagement was assessed using quantitative and/or qualitative methods. A critical evaluation of the findings enabled the construction of a public engagement ‘tick list’ for future events that would enable a hypothesis-driven approach with more effective communication activities and more robust evaluation.

Introduction Heart failure (HF) affects approximately 1 million people in the UK, adversely affecting quality of life, functional capacity and cognitive health. This results in frequent hospitalisation and significant healthcare costs1, 2. Iron deficiency complicates heart failure in approximately 50% of patients3, and is increasingly recognised as a significant contributor to morbidity in this group. Intravenous ferric carboxymaltose (FCM) has been shown to improve quality of life (New York Heart Association [NYHA] class and Kansas City Cardiomyopathy Questionnaire [KCCQ]), performance in 6-minute walk test (6MWT), reduce hospitalisations, and is reflected in international guidelines4-7. We aimed to assess the feasibility, safety and cost implications of establishing an IV iron service for patients with HF in a large tertiary cardiology centre. Method Over a 6-month period (July-December 2019), outpatients with symptomatic heart failure (NYHA class ≥2) and severe left ventricular systolic dysfunction (LVEF ≤40%) were screened, and serum iron studies performed. Patients with iron deficiency (ferritin Results Fifty-two patients underwent IV iron replacement (69% male, mean age 66 years) with no significant adverse events or hospital admissions. The financial impact to the trust was a net income of £55 per patient (FCM treatment £290, remuneration £345). Ferritin increased significantly 83.3ug/L to 433ug/L (p Conclusion Utilisation of the existing iron infusion service facilitated the delivery of IV iron replacement for patients with heart failure with little need for additional training and resources. Delivery of the service did not incur additional cost to the trust and in fact there was a small net gain. In-keeping with published data we demonstrated comparable improvements in serum iron parameters and a trend towards improvement in NYHA class. IV iron replacement with FCM is safe and affordable and should be considered in all iron deficient patients with symptomatic heart failure. Conflict of Interest Yes - recieved honorarium from Vifor

© 2020, © 2020 Informa UK Limited, trading as Taylor & Francis Group. Introduction: Pseudomonas aeruginosa is a common, ubiquitous bacterium that is found in natural environments but is also a successful opportunistic pathogen of humans and plants. The reasons for this flexibility and evolutionary success can be attributed to its ability to readily acquire new genes to ensure its survival enabling it to survive desiccation, the action of antimicrobial compounds and invade new territories such as modern hospitals with high levels of antibiotic usage. Areas covered: Literature was searched using PubMed and Web of science (05/19 to 05/20). Identified studies paint a picture of a dynamic, highly variable population shaped by frequent intra- and inter-species horizontal gene transfer resulting in a species able to resist the action of antibiotics and deploy multiple virulence strategies controlled by complex quorum-sensing systems. We investigate possible control measures including anti-virulence and environmental control measures. Expert opinion: P.aeruginosa is a resilient, richly diverse species but also a global health threat due to the emergence and global dissemination of successful multiresistant clones that resist all antibiotics. Genomics offers the potential for rapid identification of ‘high-risk’ clones to guide chemotherapy, but novel control measures are also required to slow the species progression to pan-resistance.

© 2020 Access to safe drinking water free from microbial pollution is an issue of global concern. The use of photocatalytic thin films in water treatment has focused on titanium dioxide, which requires UV-activation, proving a potential barrier to upscaling and implementation in the real world. Visible-light-activated photocatalytic thin films, such as bismuth oxide, have recently been shown to have antimicrobial properties. However, more understanding of the photocatalytic effect on the microbial population in water is required. Glass beads coated with bismuth oxide were incubated with either Microcystis aeruginosa, Anabaena sp. or free-floating genomic DNA. The presence of bismuth oxide-coated glass beads was able to rapidly stop a population of cyanobacteria from increasing. The coated beads were also able to degrade genomic DNA. Leachate from the beads showed no increase in toxicity against human liver cells. This data demonstrates the efficacy of bismuth oxide-coated glass beads for controlling potentially dangerous cyanobacterial populations, whilst potentially reducing the amount of free-floating genomic DNA (an essential issue in the face of antimicrobial resistance) – all of which should be essential considerations in emerging water treatment technologies.

In response to the accepted risk of emerging antimicrobial resistance, many organizations and institutions have developed and delivered events and activities designed to raise awareness of the issue and to change the behaviour of the intended audience. However, few of these events for a general public audience are documented or able to be sourced by those who might wish to repeat, adapt or modify, particularly those events that are successful. ‘Insider knowledge’ appears to be the best search tool. Moreover, evaluation of the success or impact of the event is rarely published. It would be useful if there were a ‘hub’ where descriptions of such activities could be deposited, enabling the building of a significant resource with real academic value.

Bi TiO /Bi Ti O composite photocatalyst was investigated as an alternative to the conventionally used titanium dioxide, to tackle the well-known drawbacks of fast charge-carriers recombination and low quantum efficiency of TiO . Polycrystalline Bi TiO /Bi Ti O thin films were produced by pulsed DC reactive magnetron co-sputtering, a method of high industrial relevance, and compared to titanium dioxide coatings produced with the same technique. Following the deposition process, optimum thermal treatment temperature and length were established, to obtain crystallisation. The synthesised coatings were thoroughly analysed with a range of techniques, including Raman spectroscopy, XRD, SEM, TEM, EDX, XPS and AFM. The photocatalytic properties of Bi TiO /Bi Ti O composite were assessed through methylene blue degradation and E. coli inactivation tests under UV-A irradiation. The results indicated considerably higher efficiency of the composite photocatalyst when compared to TiO . Moreover, the reusability assessment of Bi TiO /Bi Ti O thin films revealed an incremental performance increase after each consecutive test, leading to a 6-fold increase in photocatalytic activity between the first and 15th cycle. Time-resolved photoluminescence and XPS analysis highlighted an increased presence of oxygen vacancies, forming over repeated usage of bismuth titanate, leading to longer lifetimes of photogenerated species and enhanced photocatalytic performance. 12 20 4 3 12 2 12 20 4 3 12 12 20 4 3 12 2 12 20 4 3 12

Pseudomonas aeruginosa is a ubiquitous environmental microorganism that is also a common cause of nosocomial infections that vary in severity from chronic wound infections to pneumonia, bloodstream infections and sepsis. Its ability to survive in many different environments and persistently colonize human tissue is linked to its presence within biofilms that form on indwelling device surfaces such as plastics and stainless steel. Biofilm promotes bacterial adhesion and survival on surfaces, reduces susceptibility to desiccation, and the actions of antibiotics and disinfectants. Recent genome sequencing studies demonstrate that P. aeruginosa is a highly diverse species with a very large pan-genome consistent with its adaptability to differing environments. However, most MDR infections are caused by a small number of “high-risk” clones or lineages that recently emerged and spread globally.In our 2017 study of the resistome of P. aeruginosa we confirmed the power of genome-wide association (GWAS) techniques to explore the genetic basis of several antibiotic resistance phenotypes and discovered 46 novel putative resistance polymorphisms. In this study we sought to examine genetic associations within a subset of these isolates with simple biofilm phenotypes. We examined the genetic basis for biofilm production on polystyrene at room temperature (22°C) and body temperature (37°C) within a total of 280 isolates. 69% of isolates (n=193) produced more biofilm mass at 22°C, whilst those producing more biofilm at 37°C had reduced optical density 540 variation. We found statistically significant associations with IpxO and other genes associated with arsenic resistance to be significantly associated with this trait. IpxO which encodes a lipid A hydroxylase and arsenic reduction genes have previously been found to be associated with biofilm production in this species. We analyzed 260 ST111 and ST235 genomes and found considerable genetic variation between isolates in their content of genes previously found associated with biofilm production. This is indicative of a highly variable and flexible population within these clades with frequent emergence of successful sub-lineages. Analysis of 48 of these isolates’ ability to form biofilm on stainless steel surfaces showed that a ‘good’ biofilm-forming phenotype had significant intra-clone variation, independent of core genome phylogeny with pan-genome analysis, suggesting a possible association and involvement of components of the type IV secretion system. However, GWAS and pan-GWAS analyses yielded weaker statistical significance. This study confirms GWAS and pan-GWAS trait associations can be performed for biofilm phenotype and produce data in agreement with each other. This panel of 280 study isolates, matched to genomic data has potential for the investigation of other phenotypes in P. aeruginosa perhaps as part of a growing database / collection. A representative, curated, genome sequenced collection should increase in usefulness as it grows offering increasing statistical power.ImportanceP. aeruginosa is a major cause of multiply antibiotic infections worldwide but it is also found in many hospital and natural environments, especially aquatic ones. In this study we examined genetic polymorphism associated with biofilm production at room temperature and at body temperature, the biofilm associated gene repertoire of two major MDR clones and also genetic polymorphisms associated with biofilm production on stainless steel. Using these genome-wide and pan-genome wide association methods we identified / confirmed potential key genes involved in biofilm production and survival of P. aeruginosa. The study demonstrates the potential usefulness of large, genome sequenced isolate collections such as ours, to better understand the genetics underlying phenotypic diversity in this species.

Aims Video-assisted thoracoscopic surgery (VATS) ablation has been advocated as a treatment option for non-paroxysmal atrial fibrillation (AF) in recent guidelines. Real-life data on its safety and efficacy during a centre’s early experience are sparse. Methods and results Thirty patients (28 persistent/longstanding persistent AF) underwent standalone VATS ablation for AF by an experienced thoracoscopic surgeon, with the first 20 cases proctored by external surgeons. Procedural and follow-up outcomes were collected prospectively, and compared with 90 propensity-matched patients undergoing contemporaneous catheter ablation (CA). Six (20.0%) patients undergoing VATS ablation experienced ≥1 major complication (death n = 1, stroke n = 2, conversion to sternotomy n = 3, and phrenic nerve injury n = 2). This was significantly higher than the 1.1% major complication rate (tamponade requiring drainage n = 1) seen with CA (P

Test methods for efficacy assessment of antimicrobial coatings are not modelled on a hospital environment, and instead use high humidity (>90%) high temperature (37 °C), and no airflow. Therefore, an inoculum will not dry, resulting in an antimicrobial surface exhibiting prolonged antimicrobial activity, as moisture is critical to activity. Liquids will dry quicker in a hospital ward, resulting in a reduced antimicrobial efficacy compared to the existing test, rendering the test results artificially favourable to the antimicrobial claim of the product. This study aimed to assess how hospital room environmental conditions can affect the drying time of an inoculum, and to use this data to inform test parameters for antimicrobial efficacy testing based on the hospital ward. The drying time of different droplet sizes, in a range of environmental conditions likely found in a hospital ward, were recorded (n = 630), and used to create a model to inform users of the experimental conditions required to provide a drying time similar to what can be expected in the hospital ward. Drying time data demonstrated significant (p < 0.05) variance when humidity, temperature, and airflow were assessed. A mathematical model was created to select environmental conditions for in vitro antimicrobial efficacy testing. Drying time in different environmental conditions demonstrates that experimental set-ups affect the amount of time an inoculum stays wet, which in turn may affect the efficacy of an antimicrobial surface. This should be an important consideration for hospitals and other potential users, whilst future tests predict efficacy in the intended end-use environment.

The aim of the present paper is to assess the antimicrobial activity of novel narrow band gap semiconductor photocatalysts under visible light irradiation, compared to titanium dioxide, which is the conventionally used photocatalytic material. Bismuth oxide, bismuth tungstate and titanium dioxide coatings were prepared using pulsed DC reactive magnetron sputter deposition onto batches of 2 mm spherical glass beads that were agitated during the deposition process to ensure uniform coverage. Additional coatings were deposited onto flat glass substrates for specific analytical techniques. Following deposition, the coatings were annealed in air at 673 K for 30 min to enable crystal structure development. Annealed coatings were analysed with SEM, EDX, XRD, XPS, AFM, UV–vis spectroscopy and water contact angle measurements. The photocatalytic properties of the coatings were initially assessed via a Rhodamine B dye degradation test under visible light irradiation. Antimicrobial efficiency of the coatings was tested via inactivation of E. coli; additionally, bacterial adhesion experiments were performed for all types of the studied coatings. It was found that the performance of bismuth oxide for both dye degradation and bacterial inactivation experiments under visible light was superior to that observed for either bismuth tungstate or titanium dioxide. Moreover, bismuth oxide coatings (and to a lesser extent – bismuth tungstate), due to its hydrophobic nature was able to inhibit bacterial adhesion to the surface.

Introduction The safety and efficacy of Standalone video assisted thoracoscopic surgical (VATS) ablation for atrial fibrillation (AF) has not been established. In particular, comparative data with catheter ablation (CA) are scarce. Methods We prospectively collected data on 30 consecutive VATS AF ablations (mean age 58±10 years, 26 male) performed by an a single surgeon, the first 20 of which were proctored by external surgeons. We compared peri-procedural complications and medium-term arrhythmia free survival to those of 90 propensity-matched patients who underwent RF CA during the same time period. Results VATS and catheter patients were well matched with regard to all measured demographics (table 1). 6 (20.0%) patients undergoing VATS experienced 1 major complication (death n=1, stroke n=2, conversion to sternotomy n=3, phrenic nerve injury n=2). This was significantly higher than the 1 (1.1%) major complication rate (tamponade requiring drainage) seen with CA; p Conclusions Standalone VATS AF ablation is associated with similar success rates to catheter ablation, but with a significantly higher rate of major complications.

© 2018 Royal Society of Biology. Vaccination coverage in the United Kingdom is below the level recommended by the World Health Organisation, and when vaccination coverage is not sufficient, outbreaks of infectious diseases can occur. In 2015, coverage of the first dose of the Measles-Mumps-Rubella vaccine declined in the United Kingdom for the first time since 2008, indicating a need to raise public awareness and understanding of the importance of vaccination to public health. Identifying 16 – 18-year olds as a target audience, being future parents who would make decisions regarding vaccination of their children, a digital educational resource (‘SimFection’) was developed to deliver key messages about the spread and control of vaccine-preventable infectious diseases (identified via school curricula). The process of development utilised an iterative approach, involving a cyclic process of prototyping, testing, analysis and refinement with a range of audiences including students, schoolchildren, and trainee teachers. The completed resource is now available online for free download.

The insertion of prosthetic devices into the oral cavity affects the oral microflora and results in accumulation of microorganisms on the prosthetic surface. Such fouling of denture surfaces can lead to a number of oral diseases and consequently to the replacement of the denture. Here, we report the post-synthesis introduction of silver in zeolite-loaded dental acrylic (DAZ) resins that does not influence the mechanical or aesthetic properties of the DA resins, and provides them with a long-term antimicrobial activity. Na-FAU zeolite (2 wt%) was incorporated into DA resin, which was conventionally processed and cut into 10 mm × 20 mm × 3 mm coupons. The Na+ in the zeolite was then exchanged with Ag+ via immersion of the DAZ coupons in 0.01 M AgNO3 solution to obtain DAZ/Ag-treated coupons used in antimicrobial tests. Antimicrobial tests showed that the DAZ/Ag-treated coupons were active against Candida albicans (a reference and a clinically relevant strain), Streptococcus mutans and Fusobacterium nucleatum. Ag leaching tests on the Ag-charged coupons at 1, 2, 3, 4, 7, 14, 30 and 45 days of incubation in distilled water at 37 °C, indicated sustained release of silver. Antimicrobial tests using a reference Candida albicans strain showed that the leached coupons retained antimicrobial activity after 45 days immersion in distilled water, but, after 60 days incubation no antimicrobial activity was observed. Cytotoxicity assay results indicated that the DAZ/Ag-treated coupons showed no additional cytotoxicity compared to neat dental acrylic coupons.

A ZnO@ZIF-8 powder on a gram scale was prepared via treatment of ZIF-8 with silver nitrate to induce spontaneous formation of ZnO nanorods on the surface of the ZIF-8 crystals. The crystal structure, phase purity, and physicochemical characteristics of ZnO@ZIF-8 were determined by X-ray diffraction, high-resolution electron microscopy, energy-dispersive spectroscopy, and nitrogen adsorption. The antimicrobial potential of ZnO@ZIF-8 for reduction of microorganisms often implicated with catheter-associated urinary tract infections (CAUTIs) was studied in detail using four target pathogens, Escherichia coli, Klebsiella pneumoniae, Proteus mirabilis, and Staphylococcus aureus. The ability of the compound to kill all four microorganisms in suspension was established, and a minimum bactericidal concentration of 0.25 mg mL−1 was determined for each microorganism. ZnO@ZIF-8 compound was found to be no more toxic to Galleria mellonella than distilled water, which was assessed by injection of Galleria with 10 μL of ZnO@ZIF-8 of concentrations of up to 2 mg mL−1. ZnO@ZIF-8 suspensions (2 mg mL−1 concentration) were able to reduce well-established biofilms of all four organisms containing between 107 and 109 CFU mL−1 to below limit of detection (BLD) over a 24 h period. Silicone-embedded ZnO@ZIF-8 (2 or 4 wt % ZnO@ZIF-8 loading) also demonstrated antimicrobial properties with all four microorganisms being eliminated from the surface within 24 h. The ZnO@ZIF-8 high potency and rapid antibiofilm activity against all four test organisms coupled with its nontoxicity offer a new avenue for control of microbial colonization of catheters, which would ultimately result in reduction of CAUTIs.

The increase in Antimicrobial resistance (AMR) microorganisms has been exacerbated by exposure to antimicrobial drugs (e.g. antibiotics). A solution to AMR may require academic researchers to not only contribute to the drug discovery pipeline through laboratory research, but also to engage and inform non-specialist audiences using a variety of interventions in order to change behaviour towards our use of antibiotics. In this paper, the authors describe a hands-on public engagement event focusing on AMR. 'A Spoonful of Soil', was created by drawing on the past experiences of the delivering team (also described), with planning focusing on clear concise messages, selection of an appropriate audience and ensuring the event would be of significant interest to the audience. The event had a significant footfall of over 300 visitors. Key messages which aimed to raise awareness of AMR and educate visitors on the actions and behaviours that can help address the global issue of AMR were delivered by appropriate experts successfully, however success in reaching audience cannot be concluded from the feedback and evaluation gathered.

© 2018 Royal Society of Biology. A series of events was developed to engage audiences in discussion around the importance of microorganisms in the production of fermented foods, particularly through tasting. The events were designed to attract different audiences: families attending a science museum; families in their community space; and adults in different informal eating environments. Information was provided, alongside interactive activities where possible. Feedback was positive in that the audiences enjoyed the format, the food and the events themselves, although science itself was not always specifically mentioned. The dining experience format provided a versatile and informal opportunity for engagement between experts and non-experts, and is suggested as a valuable template for similar activities, assuming appropriate budgeting and advertising issues are addressed, alongside appropriate objective setting and effective evaluation.

Antimicrobial resistance is a global threat with catastrophic forecasts in terms of human and economic losses. The so-called ESKAPE pathogens ( Enterococcus species, Staphylococcus aureus , Klebsiella pneumoniae , Acinetobacter baumanii , Pseudomonas aeruginosa and Enterobacter species) represent a range of species of particular concern because they cause many serious hospital infections, and can show resistance toward available commercial antibiotics. Copper-containing zeolite nanocrystals (10–30 nm) with FAU-type structure (Cu-FAU), in the form of stable colloidal suspensions, were prepared at high yield in the absence of organic templates and studied for their activity against ESKAPE microorganisms. The materials were active against all six ESKAPE species. The survival of Staphylococcus aureus, Klebsiella pneumoniae and Pseudomonas aeruginosa on stainless-steel coupons after direct treatment with the Cu-FAU zeolite suspensions was determined quantitatively. Complete decontamination (5-log reduction in bacterial counts) was achieved within 20 min for P. aeruginosa , and within 10 min for the K. pneumoniae and S. aureus . This result is significant, particularly for sanitization of surfaces in healthcare settings, with the potential to initiate a new direction of research to help address the global antimicrobial resistance threat.

Listeria monocytogenes is a pathogenic bacterium, with human disease and infection linked to dairy products, seafood, ready-to-eat meat and rawundercooked meats. Stainless steel is the most common food preparation surface and therefore, it is important to understand how food storage conditions such as surface materials, temperature and relative humidity can affect survival of L. monocytogenes. In this study, survival of L. monocytogenes on stainless steel was investigated at three temperatures (4, 10 and 21°C), each approx. 11, 50 and 85% humidity. Results indicate that the lower the temperature, the more cells were recovered in all three humidity environments, while medium humidity enhances survival, irrespective of temperature. Lower humidity decreases recovery at all temperatures. These data support the guidance noted above that humidity control is important, and that lower humidity environments are less likely to support retention of viable L. monocytogenes on a stainless steel surface.Understanding survival of potential food-borne pathogens is essential for the safe production and preparation of food. While it has long been 'common knowledge' that relative humidity can affect the growth and survival of micro-organisms, this study systematically describes the survival of L. monocytogenes on stainless steel under varying humidity and temperatures for the first time. The outcomes from this paper will allow those involved with food manufacture and preparation to make informed judgement on environmental conditions relating to humidity control, which is lacking in the food standards guidelines.