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The specific Kerr constant of rabbit skeletal light meromyosin was measured in various buffer systems between pH 8.7 and 9.6 using transient electric birefringence and showed the generally expected decrease with solution conductivity. Analysis of birefringence rise times and held reversal transients showed that this decrease was connected with large changes in what appeared to be a permanent dipole moment. Comparison of the data with some theoretical predictions (O'Konski, C. T.; Krause, S. J. Phys. Chem. 1970, 74, 3243) indicated that the changes in the specific Kerr constant were most probably due to a slow induced dipole moment resulting from ion atmosphere polarization

Size exclusion chromatography (SEC) of poly(vinylpyrrolidoneco-vinylacetate), PVPVA, and poly(vinylpyrrolidone-co-dimethylamincethylmethacrylate-co-vinylcaprolactan),PVPDMMAEMAVC, is evaluated in terms of resolution between polymer and solvent peaks using aqueous and nonaqueous mobile phases. A 1:1 (v/v) water/methanol, 0.1M LiNO2 mobile phase is used with four Waters Ultrahydrogel columns with pore sizes of 120A, 500A, 1000A, and 2000A. DMF, 0.1M LiNO3 mobile phase is used with three different two column sets. Two of the column sets are comprised of a mixed bed packing of poly(styrene-co-divinyl benzene), obtained from Shodex (KD80M), followed by either an Ultrahydrogel 120A or a PLge1 100A. The third two column set is a PLge1 104A followed by a PLgel 500A. Any of the second columns in these sets improve separation between the trailing end of the polymer peak and the leading end of the solvent peak but the column set using an Ultrahydrogel 120A yields a separation of these peaks whose valley is ...