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Insects of the order Lepidoptera are among the most destructive global pests causing billions of dollars in damage annually. A new class of N-arylpyrazole-4-methylpiperidines with potent activity on lepidopteran species has been discovered.Compound 1 was identified in a high throughput insecticide screen to possess modest activity on the lepidopteran insect Plutella xylostella. Optimization of 1 to compound 42 resulted in a compound with excellent activity on Spodoptera exigua, Spodoptera frugiperda, and Helicoverpa zea with LC50 values of 2.8, 1.4, and 12.5 ppm respectively. While the mode of action remains unknown these compounds do not appear to work by many of the known biochemical mechanisms of insect control.The N-arylpyrazole-4-methylpiperidines represent a new class of insecticides with excellent activity on a broad spectrum of lepidopteran pests. Studies to date indicate the potential for a novel mode of action, however, the target site is at present unknown. This article is protected by copyright. All rights reserved.

Acoustic telemetry was used to track salmon smolts during river migration and into the open marine coastal zone. We compared migration direction and speed with particle tracking simulations to test the hypothesis that marine migration pathways are defined by active swimming current following behaviour. Habitat-specific survival rates, movement speeds, depths and directions in riverine, estuarine, and coastal habitats were also quantified. Salmon post-smolts did not disperse at random as they entered the unrestricted, coastal zone of the North sea; rather they chose a common migration pathway. This was not the most direct route to marine feeding grounds (ca. 44° N); north in the direction of the prevailing currents. Particle modelling showed that the actual post-smolt migration route was best predicted by active swimming at 1.2 body length.sec.−1 at a bearing of 70° from north but not by current following behaviour. Fish migrating in larger groups and earlier in the migration period had increased migration success. We conclude that: post-smolts have preferred migration routes that are not predicted by the shortest direction to their ultimate destination; they do not simply use the current advantage to migrate; and that they actively swim, occasionally directly against the current prevailing at the time.

Combining data from multiple acoustic telemetry studies has revealed that west coast England Atlantic salmon (Salmo salar L.) smolts use a northward migration pathway through the Irish Sea to reach their feeding grounds. 100 Atlantic salmon smolts were tagged in May 2020 in the River Derwent, northwest England as part of an Environment Agency/Natural England funded project. Three tagged smolts were detected on marine acoustic receivers distributed across two separate arrays from different projects in the Irish Sea. One fish had migrated approximately 262km in 10 days from the river mouth at Workington Harbour, Cumbria to the northernmost receiver array operated by the SeaMonitor project; this is the longest tracked marine migration of an Atlantic salmon smolt migrating from United Kingdom. This migrating fish displayed behaviours which resulted in fast northward migration. The remaining two fish were detected on a receiver array operated by a third project: “COMPASS”. These detections further provide evidence that migration to reach marine feeding grounds of salmon smolts from rivers draining into the Irish Sea is northerly. The pattern of these detections would not have been possible without the collaborative efforts of three distinct and separately funded projects to share data. Further work is required to fully understand migration trajectories in this species on the west coast of the British Isles.

We have discovered mesoionic pyrido[1,2-a]pyrimidinones as a novel class of insecticides and identified compounds such as dicloromezotiaz for development. During optimization of this area, we also discovered that mesoionic pyrido[1,2-a]pyrimidinones containing 3-biaryl groups at the 3-position of the core ring possess potent biological activity against Lepidoptera species. In this chapter, we will present the discovery, preparation, and optimization of this class of compounds. Biological activity of representative analogs will also be presented.

Determining where fish are distributed across days and seasons is valuable for understanding their ecology, evolution and conservation. The results presented here provide insight into the spatial and temporal distribution of brown trout (native salmonid species) and dace (invasive cyprinid species) in an artificially impounded section of lowland river, demonstrating that both species remain relatively local to their release point and do not exhibit wide-ranging movements from late summer into winter. Commonalities in the movement patterns were observed between the species despite their contrasting life histories, but there were also important differences observed both in their home range and activity patterns over the duration of the study. In general dace were much more active than trout. Both trout and dace exhibited clear crepuscular peaks in movement with higher displacement rates being observed during dawn and dusk periods which remained consistent over the duration of the study. Both species exhibited a high residency which may be a direct result of the artificial barrier present, promoting residency. Trout showed a significant increase in displacement rates and a drop in residency in November which may represent putative spawning behaviour. In general home range sizes remained stable over the tracking period for both species. Home range size was affected by fish length for both species, with larger individuals being more localised then smaller individuals. We propose that the diel patterns observed are primarily driven by foraging activity and opportunity which changes with seasonal influences and onset of potential spawning period and/or overwintering behaviour. This study demonstrates how data derived from telemetry studies can reveal movement behaviours of fish species associated with undertaking basic ecological requirements (feeding, shelter etc.) which are regulated by variation in the environment. Understanding the interplay between the environment and an animal’s behaviour is important from a conservation management perspective with increasing environmental pressures and predicted regime changes. From a fishery management viewpoint these data can feed into stock status monitoring in difficult to monitor impounded lowland riverine habitat and also increase our understanding of how potential human induced changes affect fish populations.

Man-made barriers have led to river fragmentation, restricting fish migrations to critical habitat. Fragmentation is relevant to the Water Framework and Habitats (Annex II fish) Directives of the European Union. SNIFFER (Water Framework Directive 111) is a United Kingdom-developed fish passability assessment method with passability scores based on published data describing the physiological abilities of different fish species/life stages. SNIFFER is an objective protocol, but final scores require assessor opinion on specific nonquantified elements. The French ICE fish passability assessment protocol covers a larger number of fish species/life stages and removes the requirement for velocity readings (except in a few situations) and expert opinion with assessors following a decision tree process. In most situations, fewer direct measurements are required for the ICE protocol, and the evaluation process is quicker and simpler. Both protocols utilize a similar passability scoring system (0 = total barrier, 0.3 = high impact, 0.6 = low impact, 1 = no risk). Comparison of outcomes for species categories for both protocols was made in paired comparisons for 112 transversal sections (fish passage routes) recorded at 52 barriers (in-river structures) of varying complexity in Irish rivers. Overall scores were found to be in high agreement for species groups at impassable (Score 0) and no risk (Score 1) barriers. Protocol agreement dropped significantly for high-impact (Score 0.3) and low-impact (Score 0.6) barriers. Results are discussed in the context of barrier passability at the 52 structures examined, primarily in the context of Atlantic salmon (Salmo salar L.) and of sea lamprey (Petromyzon marinus L.). In total, 22 of the structures had one or more fishways or fish passage solutions built into them as part of the original design. Both protocols identified substantial problems for sea lamprey and adult salmon at the majority of the fish passage solutions surveyed. The merits and shortcomings of both protocols, for managers assessing fish passability at complex riverine structures, are discussed.

Long-distance migration of Atlantic salmon (Salmo salar) is known to result in high levels of mortality. For a species experiencing global population decline, it is thus vital to better understand migration behaviour, both in the river and marine stages. Atlantic salmon smolts (n = 50) were tracked using acoustic telemetry in the River Deveron, Scotland, and adjacent coastal area. Higher rates of mortality were observed in the river (0.77% per km) than the early marine stage of migration (0.0% per km). Mortality likely resulted from predation. Higher swim speeds were recorded in the early marine stage compared with the river (marine = 7.37 ± 28.20 km/day; river = 5.03 ± 1.73 km/day [mean ± SD]), a potential predator avoidance behaviour. The majority of smolts leaving the river did so in darkness and on a flooding tide. Overall river and marine migration success were linked to nights of lower lunar brightness. Marine migration speed decreased with increasing environmental noise levels, a finding with implications for fisheries management. The migration pathway in the early marine environment did not follow obvious geographical features, such as the coastline. Thus, we suggest that early marine environment pathways are more influenced by complex water currents. These findings highlight factors that influence smolt migration survival and behaviour, areas on which future research should focus.

Rivers support some of Earth's richest biodiversity1 and provide essential ecosystem services to society2, but they are often fragmented by barriers to free flow3. In Europe, attempts to quantify river connectivity have been hampered by the absence of a harmonized barrier database. Here we show that there are at least 1.2 million instream barriers in 36 European countries (with a mean density of 0.74 barriers per kilometre), 68 per cent of which are structures less than two metres in height that are often overlooked. Standardized walkover surveys along 2,715 kilometres of stream length for 147 rivers indicate that existing records underestimate barrier numbers by about 61 per cent. The highest barrier densities occur in the heavily modified rivers of central Europe and the lowest barrier densities occur in the most remote, sparsely populated alpine areas. Across Europe, the main predictors of barrier density are agricultural pressure, density of river-road crossings, extent of surface water and elevation. Relatively unfragmented rivers are still found in the Balkans, the Baltic states and parts of Scandinavia and southern Europe, but these require urgent protection from proposed dam developments. Our findings could inform the implementation of the EU Biodiversity Strategy, which aims to reconnect 25,000 kilometres of Europe's rivers by 2030, but achieving this will require a paradigm shift in river restoration that recognizes the widespread impacts caused by small barriers.

The ryanodine receptor (RyR) is an intracellular calcium channel critical to the regulation of insect muscle contraction and the target site of diamide insecticides such as chlorantraniliprole, cyantraniliprole and flubendiamide. To-date, diamides are the only known class of synthetic molecules with high potency against insect RyRs. Target-based screening of an informer library led to discovery of a novel class of RyR activators, pyrrole-2-carboxamides. Efforts to optimize receptor activity resulted in analogs with potency comparable to that of commercial diamides when tested against RyR of the fruit fly, Drosophila melanogaster. Surprisingly, testing of pyrrole-2-carboxamides in whole-insect screens showed poor insecticidal activity, which is partially attributed to differential selectivity among insect receptors and rapid detoxification. Among various lepidopteran species field resistance to diamide insecticides has been well documented and in many cases has been attributed to a single point mutation, G4946E, of the RyR gene. As with diamide insecticides, the G4946E mutation confers greatly reduced sensitivity to pyrrole-2-carboxamides. This, coupled with findings from radioligand binding studies, indicates a shared binding domain between anthranilic diamides and pyrrole-2-carboxamides.

BACKGROUND As the world population grows towards 9 billion by 2050, it is projected that food production will need to increase by 60%. A critical part of this growth includes the safe and effective use of insecticides to reduce the estimated 20–49% loss of global crop yields owing to pests. The development of new insecticides will help to sustain this protection and overcome insecticide resistance. RESULTS A novel class of mesoionic compounds has been discovered, with exceptional insecticidal activity on a range of Hemiptera and Lepidoptera. These compounds bind to the orthosteric site of the nicotinic acetylcholine receptor and result in a highly potent inhibitory action at the receptor with minimal agonism. The synthesis, biological activity, optimization and mode of action will be discussed. CONCLUSION Triflumezopyrim insect control will provide a powerful tool for control of hopper species in rice throughout Asia. Dicloromezotiaz can provide a useful control tool for lepidopteran pests, with an underexploited mode of action among these pests. © 2016 Society of Chemical Industry

A novel class of mesoionic pyrido[1,2-a]pyrimidinones has been discovered with exceptional insecticidal activity controlling a number of insect species. In this communication, we report the part of the optimization program that led to the identification of dicloromezotiaz as a potent insecticide to control a broad range of lepidoptera. Our efforts in discovery, synthesis, structure-activity relationship elucidation, and biological activity evaluation are also presented.

A novel class of mesoionic pyrido[1,2-a]pyrimidinones has been discovered with exceptional insecticidal activity controlling a number of insect species. In this communication, we report the part of the optimization program which led to the discovery of triflumezopyrim as a highly potent insecticide controlling various hopper species. Our efforts in discovery, synthesis, structure-activity relationship elucidation, and biological activity evaluation are also presented.

Monitoring of yellow-phase and silver-phase Anguilla anguilla during their continental life history is necessary for evaluation of stock recovery measures. Eel population data for an Irish lake (Lough Sheelin) were compiled for the period 1993–2014. Catch data from 2009 to 2014 provided minimum estimates of recent silver eel production ranging annually from 0.79 to 1.84 kg/ha. Long-term changes in yellow eel abundance and silver eel size structure were assessed as part of a fishery monitoring programme. Yellow eel catch per unit effort (CPUE) in the lake (from longline surveys) was considerably higher in the 1990s (52.2–62.1 eels/100 hooks) than 2002–2008 (1.9–15.8 eels/100 hooks). Conversely, during 1993–2014, the mean size of silver eels migrating from the lake increased significantly (p

Mortality rates of wild Atlantic salmon Salmo salar smolts implanted with acoustic transmitters were assessed to determine if mortality was size dependent. The routinely accepted, but widely debated, ‘2% transmitter mass: body mass’ rule in biotelemetry was tested by extending the transmitter burden up to 12·7% of body mass in small [mean fork length (LF) 138·3 mm, range 115–168 mm] downstream migrating S. salar smolts. Over the short timescale of emigration (range 11·9–44·5 days) through the lower river and estuary, mortality was not related to S. salar size, nor was a relationship found between mortality probability and transmitter mass: body mass or transmitter length: LF ratios. This study provides further evidence that smolt migration studies can deviate from the ‘2% rule’ of thumb, to more appropriate study-specific measures, which enables the use of fishes representative of the body size in natural populations without undue effects.

This study investigated the cumulative impact of weirs on the downstream migration of wild Atlantic salmon (Salmo salar) smolts in the River Foyle, Northern Ireland. In spring of 2013 fish were released in two tributaries of similar length; one tributary (impacted) had seven low‐head weirs along the migration pathway and the other was devoid of such structures (un‐impacted). Salmon smolts fitted with acoustic transmitters were monitored via a passive acoustic telemetry array during downstream migration. In 2014 the study was repeated only in the impacted tributary. Overall freshwater survival rates were high (>94%). There was no significant difference in mortality, movement pattern, delay or travel speeds between rivers or between years at any phase of migration. Escapement of salmon smolts through Lough Foyle (a marine sea lough) to the open ocean was low, approximately 18% in each year. Escapement did not differ between impacted and un‐impacted rivers. This study showed no postpassage effects of weirs on mortality, migration speed or escapement of downstream migrating smolts. This suggests that the elevated mortality at low‐head obstacles described in other studies is not inevitable in all river systems. Migration through rivers with natural riffle‐pool migration may result in similar effects as those from low‐head weirs. Causes of apparent high mortality in the early part of marine migration in this study, are unknown; however similar studies have highlighted the impact of fish predators on smolts.

The unimpeded downstream movement patterns and migration success of small female and male Anguilla anguilla through a catchment in north-west Europe were studied using an acoustic hydrophone array along the River Finn and into the Foyle Estuary in Ireland. Twenty silver-stage A. anguilla (total length, LT, range: 332–520 mm) were trapped 152 km upstream from a coastal marine sea-lough outlet and internally tagged with acoustic transmitters of which 19 initiated downstream migration. Migration speed was highly influenced by river flow within the freshwater (FW) compartment. Anguilla anguilla activity patterns were correlated with environmental influences; light, tidal direction and lunar phase all influenced the initiation of migration of tagged individuals. Migration speed varied significantly between upstream and lower river compartments. Individuals migrated at a slower speed in transitional water and sea-lough compartments compared with the FW compartment. While 88·5% survival was recorded during migration through the upper 121 km of the river and estuary, only 26% of A. anguilla which initiated downstream migration were detected at the outermost end of the acoustic array. Telemetry equipment functioned efficiently, including in the sea-lough, so this suggests high levels of mortality during sea-lough migration, or less likely, long-term sea-lough residence by silver A. anguilla emigrants. This has important implications for eel management plans.

This study examines juvenile Anguilla anguilla (

The behaviour of returning Salmo salar (Linnaeus, 1758) approaching, and attempting to pass low-head weirs remains relatively unknown. A radio telemetry array was created at a low-head weir to enable the behaviour of S. salar (n = 120) to be observed as they approached and attempted to pass the barrier. The majority of fish successfully passed the barrier on their first or second attempt, some individuals required 11 attempts prior to successful passage occurring. Mean delay at the barrier per fish was 47.8 h (±SD 132.0 h), range 15 min to 31 days. Passage success on a fish’s initial attempt was significantly predicted by the amount of searching a fish undertook, fork length, and fat content. Fish were more likely to have a successful first passage attempt if it was smaller with a low fat content and exerted a greater effort in searching for a passage channel. Small-scale barriers cause delays and increased energy expenditure in migrating fish. Barriers may be creating an anthropogenic selection pressure for traits which enable passage success. The impact of a delay at a barrier and increased energy expenditure on reproduction and gonad development remains unknown but is likely to be negatively impacted by instream anthropogenic structures.

A series of quinoline and isoquinoline isoxazolines have been designed as pesticides for crop protection. Herein we reported the chemical synthesis, biological activity and structure–activity relationships. The isoquinoline derivative, such as 3i , is discovered as potent new class of isoxazoline insecticide which is competitive with commercial insecticide Indoxacarb .

BACKGROUND Given the physical properties of insecticides, there is often some movement of these compounds within crop plants following foliar application. In this context, movement of two formulations of cyantraniliprole, an anthranilic diamide, was characterized for translocation to new growth, distribution within a leaf and penetration through the leaf cuticle. RESULTS Upward movement of cyantraniliprole to new plant growth via the xylem was confirmed using 14C-radiolabeled cyantraniliprole and from Helicoverpa zea mortality on tomato leaves that had not been directly treated. Within a leaf there was significant acropetal movement (base to apex) of cyantraniliprole, but no significant basipetal movement (apex to base). Translaminar movement, the ability of a compound to penetrate the leaf cuticle, was demonstrated in a variety of plants, both with and without the use of adjuvants, by treating only the adaxial surface of the leaf and measuring control of diamondback moth (Plutella xylostella), green peach aphid (Myzus persicae) and sweetpotato whitefly (Bemisia tabaci) exposed in clip cages to the untreated abaxial surface. CONCLUSION The plant mobility and plant protection of cyantraniliprole is discussed with implications for use in insect resistance management and integrated pest management programs. © 2014 Society of Chemical Industry

N-Substituted amino-2(5H)-oxazolones A are a novel class of insecticides acting as nicotinic acetylcholine receptor (nAChR) agonists and show potent activity against hemipteran insect species. Here we report the discovery and preparation of this class of chemistry. Our efforts in SAR elucidation, biological activity evaluation, as well as mode-of-action studies are also presented.

Anthranilic diamides are an exceptionally active class of insect control chemistry that selectively activates insect ryanodine receptors causing mortality from uncontrolled release of calcium ion stores in muscle cells. Work in this area led to the successful commercialization of chlorantraniliprole for control of Lepidoptera and other insect pests at very low application rates. In search of lower logP analogs with improved plant systemic properties, exploration of cyano-substituted anthranilic diamides culminated in the discovery of a second product candidate, cyantraniliprole, having excellent activity against a wide range of pests from multiple insect orders. Here we report on the chemistry, biology and structure-activity trends for a series of cyanoanthranilic diamides from which cyantraniliprole was selected for commercial development.

Isoxazoline insecticides have been shown to be potent blockers of insect GABA receptors with excellent activity on a broad pest range, including Lepidoptera and Hemiptera. Herein we report on the synthesis, biological activity and mode-of-action for a class of 4-heterocyclic aryl isoxazoline insecticides.

Phenotypic variation in populations of fishes that inhabit postglacial lakes is often associated with trophic specialisations. A common sympatric foraging divergence seen in Arctic charr is into either plankton or littoral-zoobenthos feeding specialisms. In this study, we report a sympatric polymorphic Arctic charr population which is not centred on this divergence but instead manifests as a plankton (pelagic)—profundal zoobenthos foraging specialisms. The head shape of profundal fish was round and robust, the body thick set and pectoral fins long and wide. In contrast, the head of pelagic fish was pointed and slender, the body fusiform in shape and with short, narrow pectoral fins. There was no difference between profundal and pelagic fish in gill raker number. Body lipid content was significantly higher in pelagic fish as were the number or Diphyllobothrium cysts. The carbon isotope ratio was more heavily depleted in profundal fish. There was no dietary overlap in the prey items recovered from stomach contents of profundal and pelagic fish. We suggest the proximate driver behind the sympatric divergence was the successful exploitation of the profundal zone. The consequences of this have led to the development of adaptations in morphology and behaviour to support and maintain this divergence.

A novel class of mesoionic pyrido[1,2-a]pyrimidinones has been discovered with exceptional insecticidal activity controlling a number of insect species. In this communication, we report the part of the optimization program which led to the discovery of triflumezopyrim as a highly potent insecticide controlling various hopper species. Our efforts in discovery, synthesis, structure-activity relationship elucidation, and biological activity evaluation are also presented.

A novel class of mesoionic pyrido[1,2-a]pyrimidinones has been discovered with exceptional insecticidal activity controlling a number of insect species, particularly hemiptera and lepidoptera. Mode-of-action studies showed that they act on nicotinic acetylcholine receptors (nAChRs) primarily as inhibitors. Here we report the discovery, evolution, and preparation of this class of chemistry. Our efforts in structure–activity relationship elucidation and biological activity evaluation are also presented.

Infection patterns of the invasive Anguillicola crassus nematode were investigated in a population of the European eel Anguilla anguilla where parasite invasion is very recent, Loch Lomond, Scotland. Intensity levels of the parasite were associated with differences in fish ontogeny and trophic ecology. Although eels foraged on both fish and invertebrates, individuals which were smaller and fed on invertebrates (>70% contribution to diet) were found to contain a greater number of swim bladder parasites compared to larger eel with a predominance of fish (>60% contribution) in their diet. Within affected fish, a significant negative relationship was found between fish length and parasite intensity, with smaller individuals having higher parasite intensity than larger individuals. This study indicates that food intake and infection risk are linked in this recently infected host-parasite system. From a management perspective increasing our understanding of how infection intensity and repeated exposure is linked to resource use in an ecosystem is important for the future management of this endangered species in Europe.

As the world population grows towards 9 billion by 2050, it is projected that food production will need to increase by 60%. A critical part of this growth includes the safe and effective use of insecticides to reduce the estimated 20-49% loss of global crop yields owing to pests. The development of new insecticides will help to sustain this protection and overcome insecticide resistance.A novel class of mesoionic compounds has been discovered, with exceptional insecticidal activity on a range of Hemiptera and Lepidoptera. These compounds bind to the orthosteric site of the nicotinic acetylcholine receptor and result in a highly potent inhibitory action at the receptor with minimal agonism. The synthesis, biological activity, optimization and mode of action will be discussed.Triflumezopyrim insect control will provide a powerful tool for control of hopper species in rice throughout Asia. Dicloromezotiaz can provide a useful control tool for lepidopteran pests, with an underexploited mode of action among these pests. © 2016 Society of Chemical Industry.

We investigated color preference, seasonal abundance, spatial distribution and species composition of thrips in northern highbush blueberries, Vaccinium corymbosum L, in New jersey (USA). White sticky traps were more attractive to thrips compared with yellow or blue traps. Thrips captures using white sticky traps showed that their flight activity begins 20-30 d after the onset of flowering, with 10, 50 and 90% of trap captures observed at 383, 647 and 1231 degree-day accumulations, respectively (10 degrees C base temperature). Two methods were used to study thrips distribution within a blueberry bush. First, white sticky traps were placed within the bush canopy at three different heights. The highest numbers of thrips were caught on traps in the middle and top one-third of the canopy while the lowest numbers were caught in the bottom one-third. A second method determined the distribution of thrips on the blueberry plant at different heights and phenological stages. The highest numbers of thrips were found on young leaves at lower parts of the canopy, whereas flowers and fruit had fewer thrips and none were found on buds; these thrips were identified as, Scirtothrips ruthveni (88% of adults) and Frankliniella tritici (12%). The distribution of thrips within a blueberry planting was investigated using an evenly-spaced grid of white sticky traps in combination with on bush beating-tray samples. Thrips counts from traps correlated with direct counts on the bush across the entire blueberry field (macro-scale level); however, within the field (micro-scale level), there was no correlation between the number of thrips on traps and on individual bushes near traps. Early in the season, trap counts were higher on bushes closer to the forest, indicative of movement of thrips from wild hosts into blueberry fields. However, this was not the case for direct on bush counts or trap counts for the later part of the season, where there was no clear forest "edge" effect. Percent fruit injury due to thrips feeding was low, and it correlated with thrips counts on bushes but not from counts on traps. Overall, our data show that thrips counts on sticky traps need to be interpreted with care because these numbers weakly correlated with the numbers of thrips on bushes at the micro-scale level and percent fruit injury; however, they can be useful predictors of thrips activity across entire blueberry fields (macro-scale).

The leafminer Caloptilia porphyretica Braun (Lepidoptera: Gracillariidae), has become a frequent pest in commercial highbush blueberries, Vaccinium corymbosum L., in New Jersey, but little is known about its seasonal abundance, life history, and parasitism rates. Monitoring programs were conducted from 2003 to 2006 on seven blueberry farms to determine the relative abundance of this leafminer by using pheromone-baited traps and by sampling vegetative and flower/fruit clusters and new shoot growth. We found at least three distinct generations per year, with populations reaching their highest peak in the second generation. Laboratory studies characterized the life history of C. porphyretica and its parasitoid Pholetesor sp. prob. salalicus (Mason). The developmental period of C. porphyretica, from egg to adult, took 927, 838, and 912 degree-days (DD) at 20, 25, and 30°C, respectively, by using a developmental threshold of 4.8°C. This was equivalent to an average of 892 DD to complete development to...

Diamide insecticides have emerged as one of the most promising new classes of insecticide chemistry owing to their excellent insecticidal efficacy and high margins of mammalian safety. Chlorantraniliprole and flubendiamide, the first two insecticides from this class, demonstrate exceptional activity across a broad range of pests in the order Lepidoptera. This chemistry has been confirmed to control insects via activation of ryanodine receptors which leads to uncontrolled calcium release in muscle. The high levels of mammalian safety are attributed to a strong selectivity for insect over mammalian receptors.

Cost is a potential limiting factor in the adoption of mating disruption to control oriental beetle, Anomala orientalis (Waterhouse) (Coleoptera: Scarabaeidae). A 3-yr study was conducted in 1-ha blueberry, Vaccinium corymbosum L., plots to test the possibility of lowering cost by reducing the number of point-source dispensers and pheromone [(Z)-7-tetradecen-2-one] concentrations, while maintaining mating disruption. Trap shutdown, as measured by the disruption index (DI), caged females, and sentinel potted-plants with tethered females were used to assess the success of mating disruption. Disrupted plots had DI values of > or = 93% in all years, and a lower percentage of mated females, compared with control plots. However, DI values were > or = 95% at > or = 50 dispensers per ha. When 25 dispensers containing 0.05 g of active ingredient (AI) were used per hectare, the numbers of males in female cages and larvae in sentinel pots were similar to controls. Thus, dispenser density was critical for successful mating disruption of oriental beetles. Male oriental beetles approach the dispensers at all times of the day according to field observations, indicative of competitive attraction as a potential mechanism for mating disruption. However, at peak activity, greater male attraction was observed to dispensers containing 0.1 g of pheromone than 0.05 or 0.025 g, demonstrating the importance of pheromone rate. Although dispensers continued to emit pheromone for at least 7 wk in the field, emission rates dropped to levels close to 0 after 3 wk. We conclude that deployment of > or = 50 dispensers/ha at > or = 0.1 g (AI) per dispenser is the most effective rate for mating disruption of oriental beetle in blueberries.

A series of highly active fluorinated anthranilic diamide insecticides have been prepared and their biological activity assessed on two aphid species in the search for systemically active compounds that control Hemiptera. In addition, we have demonstrated a new synthesis of N-aryl 3-fluoropyrazoles.

Fly longevity is critical to sterile release programs for Mediterranean fruit flies (medflies) because the longer sterile flies are present after a release, the greater the probability of mating. Current release programs provide sterile, adult medflies with sucrose in an agar matrix for 2-3 d before release. We used cages to compare the effects of different diets on the longevity of medfly, Ceratitis capitata (Wiedemann). A diet of dry hydrolyzed yeast + sucrose supplied during the pre-release interval did not significantly affect field survivorship of medfly adult males relative to the standard sucrose diet. Post-release diets, simulating nitrogen and sugar sources that released medflies may find after release, had significant effects on medfly survivorship. Hydrolyzed yeast + sucrose resulted in the highest medfly survivorship, followed by sucrose, and then water alone. Finally, diets containing hydrolyzed yeast were not found to have significant amounts of protein and thus are more likely nitr...

A series of greenhouse and field assays evaluated two entomopathogenic nematode species and two neonicotinoid insecticides for remedial control of third-instar oriental beetle, Anomala (= Exomala ) orientalis (Waterhouse), in highbush blueberry. In two greenhouse and two field assays, Steinernema scarabaei Stock & Koppenhofer provided 50–95% control, the effect of imidacloprid varied from no effect to 60% control, and Heterorhabditis bacteriophora Poinar and thiamethoxam were ineffective. The combination of imidacloprid with S. scarabaei or H. bacteriophora was not more effective than the better of imidacloprid or nematode alone. S. scarabaei , should it become commercially available, could be an effective and economic alternative to preventive control with imidacloprid.

Laboratory and field assays using insecticides for organic pest management were conducted on the blueberry maggot, Rhagoletis mendax Curran. Topical exposure of flies to spinosad (Entrust), pyrethrum (PyGanic 1.4 EC), azadirachtin (Aza-Direct), and phosmet (Imidan 70-W) resulted in significantly higher mortality compared with the water control after 2 and 24 h. After 24 h, there were no significant differences in fly mortality among treatments of Entrust, PyGanic, or Imidan, whereas fly mortality to Aza-Direct was significantly lower. Another laboratory assay evaluated mortality of flies after residual exposure to these insecticides on leaves, after 24 and 48 h. In this assay, there were no significant differences in fly mortality after 48 h among treatments of PyGanic, Aza-Direct, and the water control, whereas significantly higher fly mortality resulted from exposure to Entrust and Imidan. A repellency assay found no measurable effects of Aza-Direct. Large-scale field trials found no treatment effect for number of adults of the blueberry maggot captured in sticky traps; however, there were significantly lower levels of fruit-infesting larvae in treated plots compared with the untreated control. Spinosad bait (GF-120 NF Naturalyte Fruit Fly Bait), Entrust, and PyGanic were not different from imidacloprid (Provado 1.6 F). However, there was a significantly higher infestation in the plot treated with azadirachtin (Agroneem) compared with Provado. Overall, the insecticides evaluated in these trials showed good ability to control blueberry maggot, suggesting that they can be incorporated in a blueberry maggot management program under organic standards.

The feasibility of disrupting sexual communication in oriental beetle, Anomala orientalis (Waterhouse), was evaluated by placing 50–75 ChemTica dispensers/ha (50–75 g active ingredient/ha) that released (Z)-7-tetradecen-2-one, the major sex pheromone component of oriental beetle, or red rubber septa deployed at the same density, but loaded with only 5–7.5 g active ingredient/ha, in 2002 and 2003. In both years, pheromone trap captures in plots treated with dispensers and rubber septa were significantly lower compared with pheromone trap captures in untreated control plots. Significantly fewer grubs were found in 2002 in blueberry plants placed in the two disrupter treatments compared with untreated control plots. In 2003, fewer females recovered from disrupter plots were found mated compared with females placed in untreated control plot. These studies indicate that sexual communication in the oriental beetle can be disrupted by deploying retrievable dispensers releasing high rates of pheromone at...

The mating behavior of sterile, laboratory-reared, male Mediterranean fruit flies, Ceratitis capitata (Wiedemann), was evaluated in field-cage mating competition tests with wild flies after exposing the laboratory males to ginger root oil extract. Without exposure to ginger root oil, sterile males obtained 12.6%, 69.0%, and 72.8% of the total matings with wild females when present in 1:1, 5:1, and 10:1 ratios of sterile males to wild males, respectively. Sterile males, exposed to ginger root oil for 3 h, 1 d before mating trials, in a 1:1 ratio with wild males, achieved 62.3% of the matings with wild females. These data suggest that exposure to ginger oil can elevate sterile male mating competitiveness to a similar degree as elevated ratios of sterile to wild males. Incorporating the use of ginger root oil extract into sterile release programs may thus increase the effectiveness of the sterile insect technique, and/or allow a reduction in the number of sterile flies that are released.

A fixed receiver array was used to examine the movement patterns and space use of the European eel Anguilla anguilla in an oligotrophic Irish lake between July and September. We assessed home range size, temporal change in spatial behaviour and activity patterns of broad-headed (n = 11) and narrow-headed (n = 8) morphotypes. Broad-headed individuals displayed a larger home range (mean KUD95 (km2):0.296 ± 0.04 S.E.) in comparison to narrow-headed individuals (mean KUD95 (km2):0.143 ± 0.02 S.E.). Eel activity was strongly dependent on light conditions. Narrow-headed individuals’ movement peaks occurred at dawn and dusk in comparison to broad-headed individuals which exhibited a more stable movement pattern throughout night and into dawn, suggesting that narrow-headed eels are more crepuscular in nature whereas broad-headed individuals are more nocturnal. Lunar phase period also influenced eel movement within the lake. These results provide valuable insights into the spatio-temporal distribution of yellow eels in a lake system, demonstrating that individuality in foraging behaviour has direct influence on spatial patterns.

OxyContin (oxycodone hydrochloride controlled release) is a long-acting preparation of oxycodone that is used as an opioid analgesic to treat chronic pain conditions. We report a patient who ingested a massive quantity of OxyContin and had altered mental status, noncardiogenic pulmonary edema, and hypoventilation that proved refractory to naloxone administration. She required mechanical ventilation for 3 days before recovering completely. The severity and length of poisoning was likely related both to the quantity and formulation of the oxycodone ingested.

Given the physical properties of insecticides, there is often some movement of these compounds within crop plants following foliar application. In this context, movement of two formulations of cyantraniliprole, an anthranilic diamide, was characterized for translocation to new growth, distribution within a leaf and penetration through the leaf cuticle.Upward movement of cyantraniliprole to new plant growth via the xylem was confirmed using (14) C-radiolabeled cyantraniliprole and from Helicoverpa zea mortality on tomato leaves that had not been directly treated. Within a leaf there was significant acropetal movement (base to apex) of cyantraniliprole, but no significant basipetal movement (apex to base). Translaminar movement, the ability of a compound to penetrate the leaf cuticle, was demonstrated in a variety of plants, both with and without the use of adjuvants, by treating only the adaxial surface of the leaf and measuring control of diamondback moth (Plutella xylostella), green peach aphid (Myzus persicae) and sweetpotato whitefly (Bemisia tabaci) exposed in clip cages to the untreated abaxial surface.The plant mobility and plant protection of cyantraniliprole is discussed with implications for use in insect resistance management and integrated pest management programs.