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Until the years 1945–1946, Uničov and its surrounding area were a region with a predominately ethic German population, which was why the local archaeology, born in 1928 under the auspices of the local Town Museum, was almost exclusively German. Fortunately, the complete archive with documentation of all excavations from the era of its ‘golden age’ has survived to this day and has been digitised in recent years. With the help of period photographs and materials from this unique archive, the book narrates the history of Uničov archaeology, which is closely linked to Mizzi Manethová, one of the ‘first ladies’ of Moravian archaeology. The story is enhanced by a comprehensive overview of sites from individual cadastres in the former judicial district of Uničov, some of which are introduced through their own tales of discovery.

A dual-output thin-disk picosecond laser operating at 100 W with 1 kHz repetition rate is reported in this Letter. By electronically adjusting the amplitude of the optical seed pulses that are injected into the laser cavity, the energy extracted from the gain medium can be shared between two pulses. Amplified double pulses are subsequently spatially separated into two independent beams by a fast Pockels cell, compressed in one common compressor, and frequency-doubled with ∼70% efficiency. This approach significantly decreases strain on the optics, as well as nonlinear effects, and is advantageous for power scaling.

BACKGROUND: The evaluation of postural trunk muscle function is a critical component of clinical assessment in patients with musculoskeletal pain and dysfunction. Postural activation of the trunk muscles has been evaluated by various methods. This study evaluates the correlation between subjective assessment of postural trunk muscle function with an objective measurement of abdominal wall expansion. METHODS: Twenty-five healthy participants (16 women, 9 men, age 22.4 years) were assessed. The subjective assessment was performed by two experienced Dynamic Neuromuscular Stabilization (DNS) clinicians evaluating the quality of trunk stabilization using five postural stability tests through palpation and observation. Interrater reliability was determined using an intraclass correlation coefficients (ICC). Objective measurement was performed using a new device (DNS Brace) which externally measures abdominal wall pressure. Spearman rank correlations were calculated for both palpation and observation measures with DNS Brace data. RESULTS: The interrater reliability (ICC2,k) estimates demonstrated moderate reliability in palpation measures for three DNS tests: Hip flexion test, Diaphragm test, & Intra-abdominal pressure regulation test (IAPRT) (ICC = 0.645-0.707). For observation measures, good reliability was found in IAPRT (ICC = 0.835), and three tests demonstrated moderate reliability: Hip flexion test, Diaphragm test, & Breathing Stereotype (ICC = 0.577-0.695). Correlation analysis demonstrated several moderate to strong correlations between palpation and DNS brace values (Assessor 1): IAPRT, r(s) = 0.580, p = 0.002, Diaphragm test, r(s) = 0.543, p = 0.005, (Assessor 2): IAPRT, r(s) = 0.776, p < 0.001, Breathing Stereotype, r(s) = 0.625, p = 0.001, Diaphragm test, r(s) = 0.519, p = 0.008, Hip Flexion test, r(s) = 0.536, p = 0.006, and Arm Elevation test, r(s) = 0.460, p = 0.021. For observation, several moderate correlations were demonstrated with DNS brace values (Assessor 1): Arm Elevation test, r(s) = 0.472, p = 0.017, (Assessor 2) Diaphragm test, r(s) = 0.540, p = 0.005, IAPRT r(s) = 0.475, p = 0.016, Hip Flexion test, r(s) = 0.485, p = 0.014, and Arm Elevation, r(s) = 0.451, p = 0.024. CONCLUSION: Based on inter-rater reliability and DNS brace correlations with trained DNS professionals, the IAPRT, Diaphragm test, and Hip Flexion test may prove useful when assessing asymptomatic individuals. More research is needed in order to establish the utility of DNS brace and clinical testing both in asymptomatic and back pain populations. DNS tests must be supplemented by further examinations for definitive clinical decision making.

Context Opencast mining of minerals has a considerable impact on the landscape and local ecosystems including bird communities. Suitable landscape restoration methods can replace habitats lost due to mining activities. Objectives Our study aimed to identify how factors such as restoration management, spatial distribution and vegetation characteristics of restored habitats can affect the risk of bird nest predation. Methods In 2018 and 2019 we conducted a field experiment using 726 artificial ground bird nests installed on four spoil heaps and in the surrounding landscape of North Bohemian Brown Coal Basin in the Czech Republic. Results Nests on the post-mining sites suffered from a significantly higher risk of predation (75.4%) than nests in the surrounding landscape (60.3%), especially on technically reclaimed sites (71.8%) compared to near-natural habitats (64.7%). A generalized linear mixed model confirmed a statistically significant impact of horizontally more heterogeneous but vertically not very diversified vegetation cover of the site on a higher risk of predation of nests installed both inside and outside post-mining sites. The risk of predation of nests adjacent to the spoil heaps decreased with increasing distance of the nest from the heap. Conclusions We consider spoil heaps as centres and sources of nest predation. We interpret the significant impact of the vegetation structure on risk of predation as result of post-mining rehabilitation approach. The higher risk of nest predation on post-mining sites reflected the character of technically reclaimed habitats. We recommend a greater extent of natural restoration methods in current rehabilitation policy.

The determinants of voter turnout in local elections are examined using global cross-national time-series comparative evidence from 97 countries. Despite the vast research agenda on turnout, only a...

Post-translational modifications of proteins enable swift physiological adaptation of cells to altered growth conditions and stress. Aside from protein phosphorylation, acetylation on e-amino group...

Thin disk regenerative amplifiers have suitable characteristics to pump picosecond OPCPA. By optimizing the trigger pulse to the MZM, a dual-output laser operating at 100 W, 1 kHz repetition rate, compressed to the transform limit is reported.

We report on the progress in developing a high energy, 1 kHz OPCPA auxiliary laser (F-SYNC) which is intended to supplement the L1-Allegra beamline. The auxiliary pulses are synchronized to the main laser with femtosecond precision and arbitrary delay.

We report on the performance improvements of the L1 Allegra laser system. The pump laser upgrade with the thin-disk multipass amplifier allows for the energy ramp-up, while a newly developed synchronized auxiliary laser system provides the capability of the pump-probe experiments at arbitrary delays.

BACKGROUND: Anorectal dysfunction (ARD), especially bowel incontinence, frequently compromises the quality of life in multiple sclerosis (MS) patients. The effect of rehabilitation procedures has not been clearly established. OBJECTIVE: To determine the effect of an individualized rehabilitation approach on bowel incontinence and anorectal pressures. METHODS: MS patients with ARD underwent 6-months of individually targeted biofeedback rehabilitation. High resolution anorectal manometry (HRAM) and St. Mark’s Fecal Incontinence Scores (SMIS) were completed prior to rehabilitation, after 10 weeks of supervised physiotherapy, and after 3 months of self-treatment. RESULTS: Ten patients (50%) completed the study. Repeated measures analysis of variance (ANOVA) demonstrated significant improvement in the SMIS questionnaire over time [14.00 baseline vs. 9.70 after supervised physiotherapy vs. 9.30 after self-treatment (p = 0.005)]. No significant improvements over time were noted in any HRAM readings: maximal pressure [49.85 mmHg baseline vs. 57.60 after supervised physiotherapy vs. 60.88 after self-treatment (p = 0.58)], pressure endurance [36.41 vs. 46.89 vs. 49.95 (p = 0.53)], resting pressure [55.83, vs 52.69 vs. 51.84 (p = 0.704)], or area under the curve [230.0 vs. 520.8 vs. 501.9 (p = 0.16)]. CONCLUSIONS: The proposed individualized rehabilitation program supports a positive overall effect on anorectal dysfunction in MS patients.

We report on a 1 kHz, 515 nm laser system, based on a commercially available 230 W average power Yb:YAG thin-disk regenerative amplifier, developed for pumping one of the last optical parametric chirped pulse amplification (OPCPA) stages of the Allegra laser system at ELI Beamlines. To avoid problems with self-focusing of picosecond pulses, the 1030 nm output pulses are compressed and frequency doubled with an LBO crystal in vacuum. Additionally, development of a thermal management system was needed to ensure stable phase matching conditions at high average power. The resulting 515 nm pulses have an energy of more than 120 mJ with SHG efficiency of 60% and an average RMS stability of 1.1% for more than 8 h.

The L1 Allegra is an OPCPA-based, high average power, high repetition rate laser system pumped by thin-disc based regenerative amplifiers currently under development at the ELI-Beamlines center in Czech Republic. The repetition rate is 1 kHz, pulse duration is below 15 fs and the wavelength centered around 820 nm with a maximum design pulse energy of 100 mJ. To avoid problems with self-focusing, a large portion of the system was placed inside vacuum, including the compressors and second-harmonic crystals for the last three 1030 nm pump lasers, the final three OPA stages, and the chirped mirror compressor. In order to reach the designed output energy of the whole system, the pump lasers need to be efficient, stable, and providing enough pump power for each of the amplification stages. Pulse compression of the final three pump lasers as well as efficient conversion to the second harmonic frequency in vacuum has posed several challenges and we report on their solutions and results. The vacuum environment causes difficulties for two main reasons. The first one is laser-induced-contamination (LIC) degrading the optical surfaces of dielectric gratings, mirrors and crystals, due to the presence of degassing components contaminating the vacuum chambers. The second reason is second-harmonic generation crystal mounts heating up, requiring regular phase matching corrections by rotation of the crystal mounts. The LIC problem was solved by regular cleaning of the chambers by means of an RF-plasma source, and the heating problem was solved by implementing active temperature stabilization by means of installing thermo-electric coolers on the crystal holders. To increase the efficiency of the second-harmonic generation, beam profiles of the pump lasers had to be improved. The original Faraday rotators, present in the linearly-designed regenerative amplifiers, caused non-Gaussian beam profiles due to the self focusing inside the rotators. By using KTF crystals inside a new type of rotators, the spatial profile of the pump lasers is more Gaussian, allowing the efficiency of the SHG to be higher, almost by 25%. All the solved problems recently allowed the system to reach a short pulse output energy of 56 mJ, paving a way to reach 100 mJ successfuly in the future.

Vacuum chambers are frequently used in high-energy, high-peak-power laser systems to prevent deleterious nonlinear effects, which can result from propagation in air. In the vacuum sections of the Allegra laser system at ELI-Beamlines, we observed degradation of several optical elements due to laser-induced contamination (LIC). This contamination is present on surfaces with laser intensity above 30 G W / c m 2 with wavelengths of 515, 800, and 1030 nm. It can lead to undesired absorption on diffraction gratings, mirrors, and crystals and ultimately to degradation of the laser beam profile. Because the Allegra laser is intended to be a high-uptime source for users, such progressive degradation is unacceptable for operation. Here, we evaluate three methods of removing LIC from optics in vacuum. One of them, the radio-frequency-generated plasma cleaning, appears to be a suitable solution from the perspective of operating a reliable, on-demand source for users.

The setup of Symbiotic 8 for Test-Comp 2021 brings radical changes in the test generation for property. Similarly as in Symbiotic 7, we generate tests by running our fork of symbolic executor Klee on the analyzed program. Symbiotic 8, however, runs several instances of Klee in parallel. We run one instance of Klee on the original program and, simultaneously, we create one (intentionally unsound) program slice for every program-terminating instruction in the program and run Klee on these slices. Apart from this principal change, we also improved other components of the tool, mainly the program slicer. Further, our fork of Klee now supports symbolic pointer arithmetics and comparison of symbolic addresses.

Symbiotic 8 extends the traditional combination of static analyses, instrumentation, program slicing, and symbolic execution with one substantial novelty, namely a technique mixing symbolic execution with k-induction. This technique can prove the correctness of programs with possibly unbounded loops, which cannot be done by classic symbolic execution.Symbiotic 8 delivers also several other improvements. In particular, we have modified our fork of the symbolic executorKleeto support the comparison of symbolic pointers. Further, we have tuned the shape analysis toolPredator(integrated already inSymbiotic 7) to perform better onllvmbitcode. We have also developed a light-weight analysis of relations between variables that can prove the absence of out-of-bound accesses to arrays.

We show that a spontaneous mutation that upregulates transcription of an operon encoding ribosomal proteins and causes overproduction of the downstream-encoded α subunit (RpoA) of RNA polymerase causes global effects on gene expression levels and proteome composition of Bordetella pertussis. Nevertheless, the resulting important downregulation of the BvgAS-controlled expression of virulence factors of the whooping cough agent did not compromise its capacity to persist for prolonged periods inside primary human macrophage cells, and it even enhanced its capacity to persist in infected mouse lungs., The BvgS/BvgA two-component system controls expression of ∼550 genes of Bordetella pertussis, of which, ∼245 virulence-related genes are positively regulated by the BvgS-phosphorylated transcriptional regulator protein BvgA (BvgA∼P). We found that a single G-to-T nucleotide transversion in the 5′-untranslated region (5′-UTR) of the rplN gene enhanced transcription of the ribosomal protein operon and of the rpoA gene and provoked global dysregulation of B. pertussis genome expression. This comprised overproduction of the alpha subunit (RpoA) of the DNA-dependent RNA polymerase, downregulated BvgA and BvgS protein production, and impaired production and secretion of virulence factors by the mutant. Nonetheless, the mutant survived like the parental bacteria for >2 weeks inside infected primary human macrophages and persisted within infected mouse lungs for a longer period than wild-type B. pertussis. These observations suggest that downregulation of virulence factor production by bacteria internalized into host cells may enable persistence of the whooping cough agent in the airways. IMPORTANCE We show that a spontaneous mutation that upregulates transcription of an operon encoding ribosomal proteins and causes overproduction of the downstream-encoded α subunit (RpoA) of RNA polymerase causes global effects on gene expression levels and proteome composition of Bordetella pertussis. Nevertheless, the resulting important downregulation of the BvgAS-controlled expression of virulence factors of the whooping cough agent did not compromise its capacity to persist for prolonged periods inside primary human macrophage cells, and it even enhanced its capacity to persist in infected mouse lungs. These observations suggest that the modulation of BvgAS-controlled expression of virulence factors may occur also during natural infections of human airways by Bordetella pertussis and may possibly account for long-term persistence of the pathogen within infected cells of the airways.

Stable picosecond supercontinuum generated in long crystals is an excellent means of seeding broadband, high-energy CPA systems. The generated output energy and spectrum can be almost three times as stable as the pump for a wide range of input pulse parameters. In this work, we show this is an intrinsic property for crystals longer than the filament and for a range of input energy values. We present a description of the stability mechanism in both the visible and infrared regions together with experimental data that support the theoretical explanation.

Each patient has his/her own individual characteristics. It is given by his/her main diagnosis and its detailed description...

We present latest progress and experimental capabilities of user-oriented XUV beamline based on high harmonic generation (HHG). The focal length of the HHG was recently extended to 5 meters, upscaling the overall XUV photon flux.

We report on the current state of readiness of the 1 kHz, 15 fs L1 ALLEGRA laser system for long term reliable operation driving user experiments based on HHG. The highly automated system, based on short pulse OPCPA, currently generates pulses with energy of 30 mJ at central wavelength of 820 nm.

Does direct democracy lead to increased electoral turnout? Research in the United States has demonstrated this effect and proposed two reasons for it: (1) the process of being involved in direct political participation may educate citizens and lead to their increased long-term political engagement (this explanation draws on the theory of participatory democracy) and/or (2) underlying issues and competitive campaigning draw public interest and cause higher turnout, although this effect is election-specific and short term. Recent empirical findings overwhelmingly favor the latter hypothesis although both mechanisms seem to apply. However, research on this topic is made difficult by the fact that direct democracy and elections take place simultaneously in the United States, which makes it hard to disentangle the effects of each. We present a study from the Czech Republic where direct democracy is not tied to elections. We analyze the effects of local/municipality direct democracy and demonstrate that it leads to increased turnout in upcoming local and national elections. We demonstrate a strong effect sparked by competitive direct democracy campaigns as well as a relatively weak long-term institutional effect of direct democracy.

Three main paths are being developed within the ELI research program for transforming driving laser pulses into bursts of bright short wavelength radiation: high-order harmonic generation in gases, plasma X-ray sources and sources based on relativistic electron beams accelerated in laser plasma. For each of these research areas, dedicated beamlines are built to provide a unique combination of X-ray sources to the scientific community. The application of these beamlines has a well-defined balance between fundamental science and applications in different fields of science and technology. Here we summarize the current status of those user beamlines and we introduce new diagnostics devices developed within the implementation phase of the project, namely compact XUV spectrometer and beam profiler that is using only one fixed detector and an imaging Michelson interferometer with increased sensitivity for low density gas jet characterization.

We report on the status of the Allegra laser beamline currently being commissioned at the ELI-Beamlines facility. The beamline will be used to drive X-ray sources for research in material science, molecular physics, and biophysics, where a high repetition rate is essential. Designed for user operation, significant attention is given to stability, reliability, and automation of the system. A photo of the beamline is shown in Fig. 1(a).

The paper deals with the design and implementation of a system for monitoring the level of liquid in a remote reservoir without power supply from electric network. The hardware of the solution is based on Arduino Mega platform with GSM / GPRS sending data and level measurement using hydrostatic pressure gauge. An internet server is used to collect data in a database and to present data to users in a form of web pages. The work focuses on high precision, low power consumption and low cost of the final system. The paper describes the developed hardware and software. The paper also present testing of the system in real conditions.

The Allegra femtosecond laser system is the main driver for high harmonic and plasma x-ray secondary sources at ELI-Beamlines operating at a 1 kHz rep rate. The system is based on OPCPA technology and consists of seven amplification stages pumped by thin-disk picosecond lasers. It is designed to reach 30 mJ output in the first phase of operation and to be ramped up to 50 mJ by engaging an additional pump laser. The amplified pulse is compressed to sub-20fs by an array of chirped mirrors and higher order dispersion is pre-compensated for by a Dazzler AOPDF in the front-end. In this paper we present the overview of Allegra system and the current status of deployment with a special focus on the high average power OPCPA in vacuum.

A passive peak intensity stabilization method based on optimal combination of Kerr nonlinearity and linear dispersion is presented. By analyzing the Kerr nonlinearity with a 3D numerical model it was found that if a Gaussian laser pulse acquires a certain amount of nonlinear phase and is consequently over-compressed to have sight negative chirp, the uctuations in peak intensity caused by energy uctuations are reduced. Thus, even if the energy of laser pulses uctuates the peak intensity can be stabilized. The simulations for realistic pulse parameters show an increase in peak intensity stability by well-over an order of magnitude. We demonstrate this process experimentally using a thin disk pump laser at ELI{Beamlines