14 results on '"Jacob Trueb"'
Search Results
2. A wireless and battery-less implant for multimodal closed-loop neuromodulation in small animals
- Author
-
Wei Ouyang, Wei Lu, Yamin Zhang, Yiming Liu, Jong Uk Kim, Haixu Shen, Yunyun Wu, Haiwen Luan, Keith Kilner, Stephen P. Lee, Yinsheng Lu, Yiyuan Yang, Jin Wang, Yongjoon Yu, Amy J. Wegener, Justin A. Moreno, Zhaoqian Xie, Yixin Wu, Sang Min Won, Kyeongha Kwon, Changsheng Wu, Wubin Bai, Hexia Guo, Tzu-li Liu, Hedan Bai, Giuditta Monti, Jason Zhu, Surabhi R. Madhvapathy, Jacob Trueb, Maria Stanslaski, Elizabeth M. Higbee-Dempsey, Iwona Stepien, Nayereh Ghoreishi-Haack, Chad R. Haney, Tae-il Kim, Yonggang Huang, Roozbeh Ghaffari, Anthony R. Banks, Thomas C. Jhou, Cameron H. Good, and John A. Rogers
- Subjects
Biomedical Engineering ,Medicine (miscellaneous) ,Bioengineering ,Computer Science Applications ,Biotechnology - Published
- 2023
3. High performance dual-electrolyte magnesium–iodine batteries that can harmlessly resorb in the environment or in the body
- Author
-
Ivy Huang, Yamin Zhang, Hany M. Arafa, Shupeng Li, Abraham Vazquez-Guardado, Wei Ouyang, Fei Liu, Surabhi Madhvapathy, Joseph Woojin Song, Andreas Tzavelis, Jacob Trueb, Yeonsik Choi, William J. Jeang, Viviane Forsberg, Elizabeth Higbee-Dempsey, Nayereh Ghoreishi-Haack, Iwona Stepien, Keith Bailey, Shuling Han, Zheng Jenny Zhang, Cameron Good, Yonggang Huang, Amay J. Bandodkar, and John A. Rogers
- Subjects
Nuclear Energy and Engineering ,Renewable Energy, Sustainability and the Environment ,Environmental Chemistry ,Pollution - Abstract
High-performance eco- and bio-resorbable magnesium–iodine batteries with >1.8 V output power cardiac pacemakers, wireless environmental monitors, thermal sensors, microcontrollers, and Bluetooth systems.
- Published
- 2022
4. Author Correction: A wireless haptic interface for programmable patterns of touch across large areas of the skin
- Author
-
Yei Hwan Jung, Jae-Young Yoo, Abraham Vázquez-Guardado, Jae-Hwan Kim, Jin-Tae Kim, Haiwen Luan, Minsu Park, Jaeman Lim, Hee-Sup Shin, Chun-Ju Su, Robert Schloen, Jacob Trueb, Raudel Avila, Jan-Kai Chang, Da Som Yang, Yoonseok Park, Hanjun Ryu, Hong-Joon Yoon, Geumbee Lee, Hyoyeong Jeong, Jong Uk Kim, Aadeel Akhtar, Jesse Cornman, Tae-il Kim, Yonggang Huang, and John A. Rogers
- Subjects
Electrical and Electronic Engineering ,Instrumentation ,Electronic, Optical and Magnetic Materials - Published
- 2022
5. Real-Time UV Measurement With a Sun Protection System for Warning Young Adults About Sunburn: Prospective Cohort Study
- Author
-
Zach Christiansen, June K. Robinson, Shiv Patel, Mary J. Kwasny, John A. Rogers, Anthony Banks, Jacob Trueb, Elizabeth Gray, Jaeman Lim, Kyeongha Kwon, Seung Yun Heo, and Jeffrey B. Model
- Subjects
Adult ,medicine.medical_specialty ,Adolescent ,Ultraviolet Rays ,Health Behavior ,Psychological intervention ,Sunburn ,Health Informatics ,Information technology ,preventive medicine ,Young Adult ,030207 dermatology & venereal diseases ,03 medical and health sciences ,0302 clinical medicine ,melanoma ,medicine ,Humans ,Prospective Studies ,030212 general & internal medicine ,Young adult ,Prospective cohort study ,Pandemics ,Preventive healthcare ,Original Paper ,mobile phone ,sun protection ,SARS-CoV-2 ,business.industry ,UV dosimeter ,Incidence (epidemiology) ,COVID-19 ,Odds ratio ,medicine.disease ,T58.5-58.64 ,United States ,health promotion technology ,Skin cancer ,Public aspects of medicine ,RA1-1270 ,business ,Sunscreening Agents ,Demography - Abstract
Background Melanoma is attributable to predisposing phenotypical factors, such as skin that easily sunburns and unprotected exposure to carcinogenic UV radiation. Reducing the proportion of young adults who get sunburned may reduce the incidence of melanoma, a deadly form of skin cancer. Advances in technology have enabled the delivery of real-time UV light exposure and content-relevant health interventions. Objective This study aims to examine the feasibility of young adults performing the following tasks daily: wearing a UV dosimeter, receiving text messages and real-time UV-B doses on their smartphone, and responding to daily web-based surveys about sunburn and sun protection. Methods Young adults aged 18-39 years (n=42) were recruited in the United States in June 2020 via social media. Participants received the UV Guard sun protection system, which consisted of a UV dosimeter and a smartphone app. During 3 consecutive periods, intervention intensity increased as follows: real-time UV-B dose; UV-B dose and daily behavioral facilitation text messages; and UV-B dose, goal setting, and daily text messages to support self-efficacy and self-regulation. Data were self-reported through daily web-based surveys for 28 days, and UV-B doses were transmitted to cloud-based storage. Results Patients’ median age was 22 years (IQR 20, 29), and all patients had sun-sensitive skin. Sunburns were experienced during the study by fewer subjects (n=18) than those in the preceding 28 days (n=30). In July and August, the face was the most commonly sunburned area among 13 body locations; 52% (22/42) of sunburns occurred before the study and 45% (19/42) occurred during the study. The mean daily UV-B dose decreased during the 3 periods; however, this was not statistically significant. Young adults were most often exercising outdoors from 2 to 6 PM, walking from 10 AM to 6 PM, and relaxing from noon to 2 PM. Sunburn was most often experienced during exercise (odds ratio [OR] 5.65, 95% CI 1.60-6.10) and relaxation (OR 3.69, 95% CI 1.03-4.67) relative to those that did not exercise or relax in each category. The self-reported exit survey indicated that participants felt that they spent less time outdoors this summer compared to the last summer because of the COVID-19 pandemic and work. In addition, 38% (16/42) of the participants changed their use of sun protection based on their app-reported UV exposure, and 48% (20/42) shifted the time they went outside to periods with less-intense UV exposure. A total of 79% (33/42) of the participants were willing to continue using the UV Guard system outside of a research setting. Conclusions In this proof-of-concept research, young adults demonstrated that they used the UV Guard system; however, optimization was needed. Although some sun protection behaviors changed, sunburn was not prevented in all participants, especially during outdoor exercise. Trial Registration ClinicalTrials.gov NCT03344796; http://clinicaltrials.gov/ct2/show/NCT03344796
- Published
- 2021
6. Real-Time UV Measurement With a Sun Protection System for Warning Young Adults About Sunburn: Prospective Cohort Study (Preprint)
- Author
-
June K Robinson, Shiv Patel, Seung Yun Heo, Elizabeth Gray, Jaeman Lim, Kyeongha Kwon, Zach Christiansen, Jeffrey Model, Jacob Trueb, Anthony Banks, Mary Kwasny, and John A Rogers
- Abstract
BACKGROUND Melanoma is attributable to predisposing phenotypical factors, such as skin that easily sunburns and unprotected exposure to carcinogenic UV radiation. Reducing the proportion of young adults who get sunburned may reduce the incidence of melanoma, a deadly form of skin cancer. Advances in technology have enabled the delivery of real-time UV light exposure and content-relevant health interventions. OBJECTIVE This study aims to examine the feasibility of young adults performing the following tasks daily: wearing a UV dosimeter, receiving text messages and real-time UV-B doses on their smartphone, and responding to daily web-based surveys about sunburn and sun protection. METHODS Young adults aged 18-39 years (n=42) were recruited in the United States in June 2020 via social media. Participants received the UV Guard sun protection system, which consisted of a UV dosimeter and a smartphone app. During 3 consecutive periods, intervention intensity increased as follows: real-time UV-B dose; UV-B dose and daily behavioral facilitation text messages; and UV-B dose, goal setting, and daily text messages to support self-efficacy and self-regulation. Data were self-reported through daily web-based surveys for 28 days, and UV-B doses were transmitted to cloud-based storage. RESULTS Patients’ median age was 22 years (IQR 20, 29), and all patients had sun-sensitive skin. Sunburns were experienced during the study by fewer subjects (n=18) than those in the preceding 28 days (n=30). In July and August, the face was the most commonly sunburned area among 13 body locations; 52% (22/42) of sunburns occurred before the study and 45% (19/42) occurred during the study. The mean daily UV-B dose decreased during the 3 periods; however, this was not statistically significant. Young adults were most often exercising outdoors from 2 to 6 PM, walking from 10 AM to 6 PM, and relaxing from noon to 2 PM. Sunburn was most often experienced during exercise (odds ratio [OR] 5.65, 95% CI 1.60-6.10) and relaxation (OR 3.69, 95% CI 1.03-4.67) relative to those that did not exercise or relax in each category. The self-reported exit survey indicated that participants felt that they spent less time outdoors this summer compared to the last summer because of the COVID-19 pandemic and work. In addition, 38% (16/42) of the participants changed their use of sun protection based on their app-reported UV exposure, and 48% (20/42) shifted the time they went outside to periods with less-intense UV exposure. A total of 79% (33/42) of the participants were willing to continue using the UV Guard system outside of a research setting. CONCLUSIONS In this proof-of-concept research, young adults demonstrated that they used the UV Guard system; however, optimization was needed. Although some sun protection behaviors changed, sunburn was not prevented in all participants, especially during outdoor exercise. CLINICALTRIAL ClinicalTrials.gov NCT03344796; http://clinicaltrials.gov/ct2/show/NCT03344796
- Published
- 2020
7. Robust Visualization and Discrimination of Nanoparticles by Interferometric Imaging
- Author
-
Derin Sevenler, Jacob Trueb, Oguzhan Avci, M. Selim Ünlü, and John H. Connor
- Subjects
business.industry ,Computer science ,False positives and false negatives ,Nanoparticle ,Nanotechnology ,02 engineering and technology ,021001 nanoscience & nanotechnology ,01 natural sciences ,Article ,Atomic and Molecular Physics, and Optics ,Visualization ,010309 optics ,Interferometry ,Software ,Interferometric imaging ,0103 physical sciences ,Computer vision ,Artificial intelligence ,Electrical and Electronic Engineering ,Image sensor ,0210 nano-technology ,business ,Biosensor - Abstract
Single-molecule and single-nanoparticle biosensors are a growing frontier in diagnostics. Digital biosensors are those which enumerate all specifically immobilized biomolecules or biological nanoparticles, and thereby achieve limits of detection usually beyond the reach of ensemble measurements. Here we review modern optical techniques for single nanoparticle detection and describe the single-particle interferometric reflectance imaging sensor (SP-IRIS). We present challenges associated with reliably detecting faint nanoparticles with SP-IRIS, and describe image acquisition processes and software modifications to address them. Specifically, we describe a image acquisition processing method for the discrimination and accurate counting of nanoparticles that greatly reduces both the number of false positives and false negatives. These engineering improvements are critical steps in the translation of SP-IRIS towards applications in medical diagnostics.
- Published
- 2017
8. Beating the reaction limits of biosensor sensitivity with dynamic tracking of single binding events
- Author
-
Jacob Trueb, M. Selim Ünlü, and Derin Sevenler
- Subjects
Analyte ,FOS: Physical sciences ,Metal Nanoparticles ,Biosensing Techniques ,02 engineering and technology ,Tracking (particle physics) ,03 medical and health sciences ,Molecular recognition ,Multiplex ,Sensitivity (control systems) ,Physics - Biological Physics ,030304 developmental biology ,Detection limit ,0303 health sciences ,Nanotubes ,Multidisciplinary ,Chemistry ,DNA ,021001 nanoscience & nanotechnology ,Endpoint Assays ,Interferometry ,Biological Physics (physics.bio-ph) ,Physical Sciences ,Gold ,0210 nano-technology ,Biological system ,Biosensor - Abstract
The clinical need for ultra-sensitive molecular analysis has motivated the development of several endpoint assay technologies capable of single molecule readout. These endpoint assays are now primarily limited by the affinity and specificity of the molecular recognition agents for the analyte of interest. In contrast, a kinetic assay with single molecule readout could distinguish between low abundance, high affinity (specific analyte) and high abundance, low affinity (nonspecific background) binding by measuring the duration of individual binding events at equilibrium. Here we describe such a kinetic assay, in which individual binding events are detected and monitored during sample incubation. This method uses plasmonic gold nanorods and interferometric reflectance imaging to detect thousands of individual binding events across a multiplex solid phase sensor with a large area approaching that of leading bead-based endpoint assay technologies. A dynamic tracking procedure is used to measure the duration of each event. From this, the total rates of binding and de-binding as well as the distribution of binding event durations are determined. We observe a limit of detection of 15 femtomolar for a proof-of-concept synthetic DNA analyte in a 12-plex assay format., 16 pages, 7 figures
- Published
- 2018
9. Interferometric Reflectance Imaging Sensor using Si-based Microfluidics
- Author
-
F. Ekiz Kanik, Jacob Trueb, Derin Sevenler, N. Lortlar Ünlü, M. Selim Ünlü, J. Needham, A. Yalcin Ozkumur, Celalettin Yurdakul, and Matthew T. Geib
- Subjects
Interferometry ,Materials science ,Optical sensing ,Microfluidics ,Nanotechnology ,IRIS (biosensor) ,Sample preparation ,Sensitivity (control systems) ,Image sensor ,Reflectivity - Abstract
There is a need for biological sensing and diagnostics tools with sensitivity compared to existing state-of-the-art technologies without complicated assays, sample preparation, and bulky equipment. Our platform technology, IRIS offers kinetic analysis of biomolecular binding and detection of proteins, nucleic acids, and individual biological nanoparticles in a simple assay format and with high sensitivity. We have shown that low-cost and disposable sensor chips and microfluidic cartridges compatible with this optical sensing technology can be manufactured using standard Si processing techniques.
- Published
- 2018
10. Digital detection of biomarkers for high-sensitivity diagnostics at low-cost
- Author
-
John H. Connor, Oguzhan Avci, M. Selim Ünlü, Jacob Trueb, George G. Daaboul, Steve Scherr, Derin Sevenler, Nese Lortlar Ünlü, and Elif Seymour
- Subjects
Materials science ,Dynamic imaging ,010401 analytical chemistry ,Nanotechnology ,Single sample ,02 engineering and technology ,021001 nanoscience & nanotechnology ,01 natural sciences ,Reflectivity ,0104 chemical sciences ,Interferometry ,IRIS (biosensor) ,Sensitivity (control systems) ,Image sensor ,0210 nano-technology - Abstract
We have demonstrated Interferometric Reflectance Imaging Sensor (IRIS) with the ability to detect single nanoscale particles. By extending single-particle IRIS to in-liquid dynamic imaging, we demonstrated real-time digital detection of individual viral pathogens as well as single molecules labeled with Au nanoparticles. With this technique we demonstrate real-time simultaneous detection of multiple targets in a single sample, as well as quantitative dynamic detection of individual biomolecular interactions for reaction kinetics measurements. This approach promises to simplify and reduce the cost of rapid diagnostics.
- Published
- 2016
11. Real-Time Capture and Visualization of Individual Viruses in Complex Media
- Author
-
Bennett B. Goldberg, M. Selim Ünlü, John H. Connor, George G. Daaboul, Derin Sevenler, Helen E. Fawcett, Jacob Trueb, and Steven M. Scherr
- Subjects
0301 basic medicine ,Serum ,Antibody microarray ,Microfluidics ,General Physics and Astronomy ,Nanotechnology ,02 engineering and technology ,Biosensing Techniques ,Article ,03 medical and health sciences ,Vesicular Stomatitis ,General Materials Science ,Image sensor ,Immunoassay ,biology ,General Engineering ,Vesiculovirus ,021001 nanoscience & nanotechnology ,biology.organism_classification ,Ebolavirus ,Recombinant Proteins ,Visualization ,Fluorescent labelling ,030104 developmental biology ,Vesicular stomatitis virus ,0210 nano-technology ,Biosensor ,Antibodies, Immobilized - Abstract
Label-free imaging of individual viruses and nanoparticles directly in complex solutions is important for virology research and biosensing applications. A successful visualization technique should be rapid, sensitive, and inexpensive, while needing minimal sample preparation or user expertise. Current approaches typically require fluorescent labeling or the use of an electron microscope, which are expensive and time-consuming to use. We have developed an imaging technique for real-time, sensitive, and label-free visualization of viruses and nanoparticles directly in complex solutions such as serum. By combining the advantages of a single-particle reflectance imaging sensor, with microfluidics, we perform real-time digital detection of individual 100 nm vesicular stomatitis viruses as they bind to an antibody microarray. Using this approach, we have shown capture and visualization of a recombinant vesicular stomatitis virus Ebola model (rVSV-ZEBOV) at 100 PFU/mL in undiluted fetal bovine serum in less than 30 min.
- Published
- 2016
12. An interferometric reflectance imaging sensor for point of care viral diagnostics
- Author
-
David S. Freedman, George G. Daaboul, Ahmet Tuysuzoglu, John H. Connor, Carlos López, Alexander P. Reddington, Helen E. Fawcett, Jacob Trueb, W. Clem Karl, and M. Selim Ünlü
- Subjects
Engineering ,business.industry ,Interface (computing) ,Point-of-Care Systems ,Real-time computing ,Biomedical Engineering ,Primary care ,Biosensing Techniques ,Equipment Design ,Viral test ,Reflectivity ,Article ,Data acquisition ,Interferometry ,Virus Diseases ,Viruses ,Humans ,Nanoparticles ,Image sensor ,Medical diagnosis ,business ,Software ,Point of care ,Biomedical engineering - Abstract
The use of in vitro diagnostic devices is transitioning from the laboratory to the primary care setting to address early disease detection needs. Time critical viral diagnoses are often made without support due to the experimental time required in today's standard tests. Available rapid point of care (POC) viral tests are less reliable, requiring a follow-on confirmatory test before conclusions can be drawn. The development of a reliable POC viral test for the primary care setting would decrease the time for diagnosis leading to a lower chance of transmission and improve recovery. The single particle interferometric reflectance imaging sensor (SP-IRIS) has been shown to be a sensitive and specific-detection platform in serum and whole blood. This paper presents a step towards a POC viral assay through a SP-IRIS prototype with automated data acquisition and analysis and a simple, easy-to-use software interface. Decreasing operation complexity highlights the potential of SP-IRIS as a sensitive and specific POC diagnostic tool. With the integration of a microfluidic cartridge, this automated instrument will allow an untrained user to run a sample-to-answer viral assay in the POC setting.
- Published
- 2013
13. A portable, pressure driven, room temperature nucleic acid extraction and storage system for point of care molecular diagnostics
- Author
-
Jacob Trueb, Mark Mazzochette, Catherine M. Klapperich, Francis Jareczek, Andre Sharon, Samantha Byrnes, Andy Fan, Alexis F. Sauer-Budge, and Publica
- Subjects
Detection limit ,Materials science ,business.industry ,General Chemical Engineering ,General Engineering ,Nanotechnology ,Molecular diagnostics ,Article ,Analytical Chemistry ,Cartridge ,Orders of magnitude (specific energy) ,Computer data storage ,Nucleic acid ,Solid phase extraction ,business ,Process engineering ,Point of care - Abstract
Many new and exciting portable HIV viral load testing technologies are emerging for use in global medicine. While the potential to provide fast, isothermal, and quantitative molecular diagnostic information to clinicians in the field will soon be a reality, many of these technologies lack a robust front end for sample clean up and nucleic acid preparation. Such a technology would enable many different downstream molecular assays. Here, we present a portable system for centrifuge-free room temperature nucleic acid extraction from small volumes of whole blood (70 mL), using only thermally stable reagents compatible with storage and transport in low resource settings. Quantitative reverse transcriptase polymerase chain reaction (qRT-PCR) analysis of simulated samples demonstrate a lower limit of detection of 1000 copies per mL, with the ability to detect differences in viral load across four orders of magnitude. The system can also be used to store extracted RNA on detachable cartridges for up to one week at ambient temperature, and can be operated using only hand generated air pressure.
- Published
- 2013
14. A point-of-care diagnostic prototype for high-throughput, multiplexed single-virus detection
- Author
-
David S. Freedman, M.S. Unlu, Helen E. Fawcett, George G. Daaboul, Jacob Trueb, John H. Connor, and Alexander P. Reddington
- Subjects
Ebola virus ,business.industry ,West Nile virus ,viruses ,medicine.disease_cause ,Reflectivity ,Virology ,Virus ,Virus detection ,Pandemic ,medicine ,IRIS (biosensor) ,business ,Point of care - Abstract
Attention to viral infections has increased after multiple recent outbreaks: the global 2009 H1N1 influenza pandemic, the variant-strain Ebola virus epidemic of 2007, and the West Nile virus epidemic of 2003–2004. Despite advances in antiviral drugs, diagnostics have lagged behind [1] [2]. These outbreaks underline the growing need for early virus discovery through rapid and reliable point-of-care (POC) diagnostics. POC diagnostics are limited to symptomatic patients as they rely on antibody-antigen interactions for detection. Plaque titer and real-time PCR are the standard methods for pre-seroconversion detection of an infection. Currently, these methods are laborious and do not lend well to POC applications. The Interferometric Reflectance Imaging Sensor (IRIS) is a portable, label-free, and multiplexed method for pre-seroconversion detection of single viral particles.
- Published
- 2012
Catalog
Discovery Service for Jio Institute Digital Library
For full access to our library's resources, please sign in.