95 results on '"Ja-Young Jang"'
Search Results
2. A FOXO1-dependent transcription network is a targetable vulnerability of mantle cell lymphomas
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Ja-Young Jang, Inah Hwang, Heng Pan, Jun Yao, Lapo Alinari, Eddie Imada, Claudio Zanettini, Michael J. Kluk, Yizhe Wang, Yunkyoung Lee, Hua V. Lin, Xiangao Huang, Maurizio Di Liberto, Zhengming Chen, Karla V. Ballman, Lewis C. Cantley, Luigi Marchionni, Giorgio Inghirami, Olivier Elemento, Robert A. Baiocchi, Selina Chen-Kiang, Sandro Belvedere, Hongwu Zheng, and Jihye Paik
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Oncology ,Medicine - Abstract
Targeting lineage-defined transcriptional dependencies has emerged as an effective therapeutic strategy in cancer treatment. Through screening for molecular vulnerabilities of mantle cell lymphoma (MCL), we identified a set of transcription factors (TFs) including FOXO1, EBF1, PAX5, and IRF4 that are essential for MCL propagation. Integrated chromatin immunoprecipitation and sequencing (ChIP-Seq) with transcriptional network reconstruction analysis revealed FOXO1 as a master regulator that acts upstream in the regulatory TF hierarchy. FOXO1 is both necessary and sufficient to drive MCL lineage commitment through supporting the lineage-specific transcription programs. We further show that FOXO1, but not its close paralog FOXO3, can reprogram myeloid leukemia cells and induce B-lineage gene expression. Finally, we demonstrate that cpd10, a small molecule identified from an enriched FOXO1 inhibitor library, induces a robust cytotoxic response in MCL cells in vitro and suppresses MCL progression in vivo. Our findings establish FOXO1 inhibition as a therapeutic strategy targeting lineage-driven transcriptional addiction in MCL.
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- 2022
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3. Anti-inflammatory effect of egg white-chalcanthite and purple bamboo salts mixture on arthritis induced by monosodium iodoacetate in Sprague-Dawley rats
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Tae-Hee Lee, Hyun-kyung Song, Ja-Young Jang, Dong-Yoon Kim, Hyun-Kyung Park, Eun-A Choi, and Beom-Seok Han
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arthritis ,purple bamboo salts (PBS) ,egg white-chalcanthite (EC) ,monosodium iodoacetate (MIA) ,Medicine (General) ,R5-920 ,Biology (General) ,QH301-705.5 - Abstract
Abstract The aim of this study is to investigate the potential of anti-osteoarthritis effects on egg white-chalcanthite (EC), purple bamboo salts (PBS), and a mixture of EC and PBS (EC+PBS). EC is a mixture of egg white and pulverized chalcanthite. PBS has been widely used as one of functional foods in Korea and shows unique features compared with common salt. Osteoarthritis was induced by intra-articular injection of monosodium iodoacetate (MIA, 4mg/kg bw) in Sprague-Dawley (SD) rats. Test substances were administered once daily for 6 weeks at doses of 10 mg EC, EC+100 mg PBS, EC+200 mg PBS before and after MIA injection. Each substance was assessed by blood chemistry parameters, and by serum cytokines including IL-1β and IL-6, and nitric oxide (NO) and prostaglandin-E2 (PGE2). Structural changes of articular cartilage were also evaluated by histopathological examination. As a result, body weight and blood chemistry parameter were not different in all experimental groups. EC+PBS mixture reduced the production of PGE2, NO, IL-1β, and IL-6. In histological grade of osteoarthritis, EC+PBS mixture had a tendency to ameliorate damage of articular cartilage induced by MIA in a dose-dependent manner. In conclusion, EC+PBS mixture was demonstrated to have a potential for anti-inflammatory effect against osteoarthritis induced by MIA in a dose-dependent manner.
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- 2016
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4. Lactobacillus sakei WIKIM30 Ameliorates Atopic Dermatitis-Like Skin Lesions by Inducing Regulatory T Cells and Altering Gut Microbiota Structure in Mice
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Min-Sung Kwon, Seul Ki Lim, Ja-Young Jang, Jieun Lee, Hyo Kyeong Park, Namhee Kim, Misun Yun, Mi-Young Shin, Hee Eun Jo, Young Joon Oh, Seong Woon Roh, and Hak-Jong Choi
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atopic dermatitis ,lactic acid bacteria ,Lactobacillus sakei ,regulatory T cells ,gut microbiota ,kimchi ,Immunologic diseases. Allergy ,RC581-607 - Abstract
Lactobacillus sakei WIKIM30 is a Gram-positive facultative anaerobic bacterium isolated from kimchi, a Korean fermented vegetable food. In this study, we found that WIKIM30 promoted regulatory T cell (Treg) differentiation by inducing dendritic cells with tolerogenic properties. The production of the T helper (Th) 2-associated cytokine interleukin (IL)-4 was decreased, but that of the Treg-associated cytokine IL-10 was increased in splenocytes from ovalbumin-sensitized mice treated with WIKIM30. We also investigated the inhibitory capacity of WIKIM30 on the development of 2,4-dinitrochlorobenzene-induced atopic dermatitis (AD), a Th2-dominant allergic disease in mice. Oral administration of L. sakei WIKIM30 significantly reduced AD-like skin lesions and serum immunoglobulin E and IL-4 levels while decreasing the number of CD4+ T cells and B cells and the levels of Th2 cytokines (IL-4, IL-5, and IL-13) in peripheral lymph nodes and enhancing Treg differentiation and IL-10 secretion in mesenteric lymph nodes. In addition, WIKIM30 modulated gut microbiome profiles that were altered in AD mice, which showed increases in Arthromitus and Ralstonia and a decrease in Ruminococcus abundance. These changes were reversed by WIKIM30 treatment. Notably, the increase in Ruminococcus was highly correlated with Treg-related responses and may contribute to the alleviation of AD responses. Together, these results suggest that oral administration of L. sakei WIKIM30 modulates allergic Th2 responses enhancing Treg generation and increases the relative abundance of intestinal bacteria that are positively related to Treg generation, and therefore has therapeutic potential for the treatment of AD.
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- 2018
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5. Allium hookeri Root Extract Inhibits Adipogenesis by Promoting Lipolysis in High Fat Diet-Induced Obese Mice
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Hyun Ju Kim, Min-Jung Lee, Ja-Young Jang, and Sung-Hyen Lee
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Allium hookeri root ,anti-obesity ,adipogenesis ,lipolysis ,high fat diet ,Nutrition. Foods and food supply ,TX341-641 - Abstract
Allium hookeri (AH) is widely consumed as a herbal medicine. It possesses biological activity against metabolic diseases. The objective of this study was to investigate effects of AH root water extract (AHR) on adipogenesis in 3T3-L1 cells and in high-fat diet (HFD)-induced obese mice. AHR inhibited lipid accumulation during adipocyte differentiation by downregulation of gene expression, such as hormone sensitive lipase (HSL), lipoprotein lipase (LPL) and an adipogenic gene, CCAAT/enhancer binding protein-α in 3T3-L1 preadipocytes. Oral administration of AHR significantly suppressed body weight gain, adipose tissue weight, serum leptin levels, and adipocyte cell size in HFD-induced obese mice. Moreover, AHR significantly decreased hepatic mRNA expression levels of cholesterol synthesis genes, such as 3-hydroxy-3-methylglutaryl CoA reductase, sterol regulatory element-binding transcription factor (SREBP)-2, and low-density lipoprotein receptor, as well as fatty acid synthesis genes, such as SREBP-1c and fatty acid synthase. Serum triglyceride levels were also lowered by AHR, likely as a result of the upregulating gene involved in fatty acid β-oxidation, carnitine palmitoyltransferase 1a, in the liver. AHR treatment activated gene expression of peroxisome proliferator-activated receptor-γ, which might have promoted HSL and LPL-medicated lipolysis, thereby reducing white adipose tissue weight. In conclusion, AHR treatment can improve metabolic alterations induced by HFD in mice by modifying expression levels of genes involved in adipogenesis, lipogenesis, and lipolysis in the white adipose tissue and liver.
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- 2019
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6. Erratum to: Allium hookeri root extract exerts anti-inflammatory effects by nuclear factor-κB down-regulation in lipopolysaccharide-induced RAW264.7 cells
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Ja-Young Jang, Min-Jung Lee, Bo-Ram You, Jong-Sik Jin, Sung-Hyen Lee, Ye-Rang Yun, and Hyun Ju Kim
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Other systems of medicine ,RZ201-999 - Published
- 2017
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7. Specific nephrotoxicity and cardiotoxicity of BT-CAL®, Sigma Anti-bonding Molecule Calcium Carbonate, in mice
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Ja-Young Jang, Jingmei Cai, Jihyun Kim, Jangbeen Kyung, Dajeong Kim, Ehn-Kyoung Choi, Youngeun Kim, Kwang-Sei Kim, Dongsun Park, Hyun-Gu Kang, and Yun-Bae Kim
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Medicine (General) ,R5-920 ,Biology (General) ,QH301-705.5 - Published
- 2016
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8. Anti-atherosclerotic effects of perilla oil in rabbits fed a high-cholesterol diet
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Yeseul Cha, Ja Young Jang, Young-Hwan Ban, Haiyu Guo, Kyungha Shin, Tae-Su Kim, Sung-Pyo Lee, Jieun Choi, Eun-Suk An, Da-Woom Seo, Jung-Min Yon, Ehn-Kyoung Choi, and Yun-Bae Kim
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Hypercholesterolemia ,atherosclerosis ,fatty liver ,lipid peroxidation ,perilla oil ,Medicine (General) ,R5-920 ,Biology (General) ,QH301-705.5 - Abstract
Abstract Anti-atherosclerosis effects of perilla oil were investigated, in comparison with lovastatin, in rabbits fed a high-cholesterol diet (HCD). Hypercholesterolemia was induced in rabbits by feeding the HCD containing 0.5% cholesterol and 1% corn oil, and perilla oil (0.1 or 0.3%) was added to the diet containing 0.5% cholesterol for 10 weeks. HCD greatly increased blood total cholesterol and low-density lipoproteins, and caused thick atheromatous plaques, covering 74% of the aortic wall. Hyper-cholesterolemia also induced lipid accumulation in the liver and kidneys, leading to lipid peroxidation. Perilla oil not only attenuated hypercholesterolemia and atheroma formation, but also reduced fat accumulation and lipid peroxidation in hepatic and renal tissues. The results indicate that perilla oil prevents atherosclerosis and fatty liver by controlling lipid metabolism, and that it could be the first choice oil to improve diet-induced metabolic syndrome.
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- 2016
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9. The FOXO1TAD-p300-Mediated Lineage Survival Transcription Program Is a Targetable Dependency of Mantle Cell Lymphoma
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Ja-Young Jang, Heng Pan, Maurizio Di Liberto, Lapo Alinari, Giorgio Inghirami, Robert A. Baiocchi, Olivier Elemento, Selina Chen-Kiang, and Jihye Paik
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Immunology ,Cell Biology ,Hematology ,Biochemistry - Published
- 2022
10. Therapy-Induced Transdifferentiation Promotes Glioma Growth Independent of EGFR Signaling
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Jun Yao, Inah Hwang, Hongwu Zheng, J. Li, Qianghu Wang, Lingxiang Wu, Baoli Hu, Dongqing Cao, Ja Young Jang, Haoqiang Ying, Yu Yao, Hwanhee Oh, and Jihye Paik
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Male ,0301 basic medicine ,Cancer Research ,Datasets as Topic ,Apoptosis ,Transcriptome ,Mice ,0302 clinical medicine ,Antineoplastic Combined Chemotherapy Protocols ,RNA-Seq ,Epidermal growth factor receptor ,Mice, Knockout ,education.field_of_study ,biology ,Brain Neoplasms ,Transdifferentiation ,Glioma ,Prognosis ,Progression-Free Survival ,ErbB Receptors ,Gene Expression Regulation, Neoplastic ,Oncology ,030220 oncology & carcinogenesis ,Female ,Signal Transduction ,Slug ,Population ,Transforming Growth Factor beta1 ,Erlotinib Hydrochloride ,03 medical and health sciences ,Downregulation and upregulation ,Cell Line, Tumor ,medicine ,Animals ,Humans ,education ,Adaptor Proteins, Signal Transducing ,Cell Proliferation ,Homeodomain Proteins ,Mesenchymal stem cell ,YAP-Signaling Proteins ,medicine.disease ,biology.organism_classification ,Xenograft Model Antitumor Assays ,Disease Models, Animal ,030104 developmental biology ,Drug Resistance, Neoplasm ,Cell Transdifferentiation ,Cancer research ,biology.protein ,Neoplasm Recurrence, Local ,Transcription Factors - Abstract
EGFR is frequently amplified, mutated, and overexpressed in malignant gliomas. Yet the EGFR-targeted therapies have thus far produced only marginal clinical responses, and the underlying mechanism remains poorly understood. Using an inducible oncogenic EGFR-driven glioma mouse model system, our current study reveals that a small population of glioma cells can evade therapy-initiated apoptosis and potentiate relapse development by adopting a mesenchymal-like phenotypic state that no longer depends on oncogenic EGFR signaling. Transcriptome analyses of proximal and distal treatment responses identified TGFβ/YAP/Slug signaling cascade activation as a major regulatory mechanism that promotes therapy-induced glioma mesenchymal lineage transdifferentiation. Following anti-EGFR treatment, TGFβ secreted from stressed glioma cells acted to promote YAP nuclear translocation that stimulated upregulation of the pro-mesenchymal transcriptional factor SLUG and subsequent glioma lineage transdifferentiation toward a stable therapy-refractory state. Blockade of this adaptive response through suppression of TGFβ-mediated YAP activation significantly delayed anti-EGFR relapse and prolonged animal survival. Together, our findings shed new insight into EGFR-targeted therapy resistance and suggest that combinatorial therapies of targeting both EGFR and mechanisms underlying glioma lineage transdifferentiation could ultimately lead to deeper and more durable responses. Significance: This study demonstrates that molecular reprogramming and lineage transdifferentiation underlie anti-EGFR therapy resistance and are clinically relevant to the development of new combinatorial targeting strategies against malignant gliomas with aberrant EGFR signaling.
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- 2021
11. Human Placenta Hydrolysate Promotes Liver Regeneration via Activation of the Cytokine/Growth Factor-Mediated Pathway and Anti-oxidative Effect
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Jae-Won Joh, Beom Seok Han, Chang Taek Oh, Isaac Lee, Jong Man Kim, Ja Young Jang, Gyu-Seong Choi, Jeom Yong Kim, Dong Sun Park, Hae Jung Han, and Tae Hee Lee
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0301 basic medicine ,Pharmacology ,chemistry.chemical_classification ,Cell growth ,Chemistry ,medicine.medical_treatment ,Glutathione peroxidase ,Growth factor ,Regeneration (biology) ,Pharmaceutical Science ,General Medicine ,Liver regeneration ,Cell biology ,03 medical and health sciences ,030104 developmental biology ,0302 clinical medicine ,Cytokine ,medicine.anatomical_structure ,030220 oncology & carcinogenesis ,Hepatocyte ,medicine ,Hepatocyte growth factor ,medicine.drug - Abstract
Liver regeneration is a very complex process and is regulated by several cytokines and growth factors. It is also known that liver transplantation and the regeneration process cause massive oxidative stress, which interferes with liver regeneration. The placenta is known to contain various physiologically active ingredients such as cytokines, growth factors, and amino acids. In particular, human placenta hydrolysate (hPH) has been found to contain many amino acids. Most of the growth factors found in the placenta are known to be closely related to liver regeneration. Therefore, in this study, we investigated whether hPH is effective in promoting liver regeneration in rats undergoing partial hepatectomy. We confirmed that cell proliferation was significantly increased in HepG2 and human primary cells. Hepatocyte proliferation was also promoted in partial hepatectomized rats by hPH treatment. hPH increased liver regeneration rate, double nucleic cell ratio, mitotic cell ratio, proliferating cell nuclear antigen (PCNA), and Ki-67 positive cells in vivo as well as interleukin (IL)-6, tumor necrosis factor alpha (TNF-α), and hepatocyte growth factor (HGF). Moreover, Kupffer cells secreting IL-6 and TNF-α were activated by hPH treatment. In addition, hPH reduced thiobarbituric acid reactive substances (TBARs) and significantly increased glutathione (GSH), glutathione peroxidase (GPx), and superoxide dismutase (SOD). Taken together, these results suggest that hPH promotes liver regeneration by activating cytokines and growth factors associated with liver regeneration and eliminating oxidative stress.
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- 2019
12. Rotavirus-mediated alteration of gut microbiota and its correlation with physiological characteristics in neonatal calves
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Hak Jong Choi, Ja-Young Jang, Jinho Park, Min-Sung Kwon, Ji Eun Lee, Ru-Hui Song, Suhee Kim, and Do-Hyeon Yu
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DNA, Bacterial ,Diarrhea ,Male ,Rotavirus ,Cattle Diseases ,Gut flora ,medicine.disease_cause ,Applied Microbiology and Biotechnology ,Microbiology ,Rotavirus Infections ,03 medical and health sciences ,Feces ,fluids and secretions ,physiological parameters ,Escherichia ,Lactobacillus ,RNA, Ribosomal, 16S ,medicine ,Microbial Systematics and Evolutionary Microbiology ,Animals ,Phylogeny ,030304 developmental biology ,0303 health sciences ,biology ,gut microbiota ,Bacteria ,030306 microbiology ,General Medicine ,Biodiversity ,Sequence Analysis, DNA ,biology.organism_classification ,Gastrointestinal Microbiome ,Blood chemistry ,Cattle ,Female ,medicine.symptom ,Bacteroides - Abstract
Diarrhea is a fatal disease to neonatal calves, and rotavirus is the main pathogen associated with neonatal calf diarrhea. Although previous studies have reported that the gut microbiota is changed in calves during diarrhea, less is known about whether rotavirus infection alters the structure of the gut microbiota. Here, we characterized fecal microbial communities and identified possible relationships between the gut microbiota profiles and physiological parameters. Five fecal specimens of rotavirus-infected calves from 1 to 30 days after birth and five fecal specimens of age-matched healthy calves were used for the microbial community analysis using the Illumina MiSeq sequencer. Rotavirus infection was associated with reduced rotavirus infection significantly reduced the richness and diversity of the bacterial community. Weighted unique fraction metric analysis exhibited significant differences in community membership and structure between healthy and rotavirus-infected calves. Based on relative abundance analysis and linear discriminant analysis effect size, we found that the representative genera from Lactobacillus, Subdoligranulum, Blautia, and Bacteroides were closely related to healthy calves, while the genera Escherichia and Clostridium were closely affiliated to rotavirus-infected calves. Furthermore, canonical correlation analysis and Pearson correlation coefficient results revealed that the increased relative abundances of Lactobacillus, Subdoligranulum, and Bacteroides were correlated with normal levels of physiological characteristics such as white blood cells, blood urea nitrogen, serum amyloid protein A, and glucose concentration in serum. These results suggest that rotavirus infection alters the structure of the gut microbiota, correlating changes in physiological parameters. This study provides new information on the relationship between gut microbiota and the physiological parameters of rotavirus-mediated diarrheic calves.
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- 2018
13. NABi, a novel β-sheet breaker, inhibits Aβ aggregation and neuronal toxicity: Therapeutic implications for Alzheimer's disease
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Hyangshuk Rhim, Seongman Kang, and Ja Young Jang
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0301 basic medicine ,Programmed cell death ,Amyloid beta ,SOD1 ,Biophysics ,Nanotechnology ,Protein aggregation ,Protein Aggregation, Pathological ,Biochemistry ,Protein Structure, Secondary ,Pathogenesis ,Superoxide dismutase ,Mice ,03 medical and health sciences ,Alzheimer Disease ,Cell Line, Tumor ,Animals ,Humans ,Cytotoxicity ,Molecular Biology ,Neurons ,Amyloid beta-Peptides ,biology ,Superoxide Dismutase ,In vitro ,Cell biology ,030104 developmental biology ,biology.protein - Abstract
Amyloid beta (Aβ) aggregates are an important therapeutic target for Alzheimer's disease (AD), a fatal neurodegenerative disease. To date, AD still remains a big challenge due to no effective treatments. Based on the property that Aβ aggregates have the cross-β-structure, a common structural feature in amyloids, we systemically designed the Aβ-aggregation inhibitor that maintains Aβ-interacting ability but removes toxic part from SOD1 (superoxide dismutase 1)-G93A. We identified NABi (Natural Aβ Binder and Aβ-aggregation inhibitor) composed of β2-3 strands, a novel breaker of Aβ aggregation, which does not self-aggregate and has no cytotoxicity at all. The NABi blocks Aβ-fibril formation in vitro and in vivo and prevents neuronal cell death, a hallmark of AD pathogenesis. Such anti-amyloidogenic properties can provide novel strategies for treating AD. Furthermore, our study provides molecular insights into the design of amyloidogenic inhibitors to cure various neurodegenerative and amyloid-associated diseases, as NABi would regulate aggregation of other toxic β-sheet proteins other than Aβ.
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- 2018
14. FOXO1 Dependent Transcription Network Is a Targetable Vulnerability of Mantle Cell Lymphoma
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Ja-Young Jang, Inah Hwang, Heng Pan, Michael Kluk, Jun Yao, Xiangao Huang, Maurizio Di Liberto, Zhengming Chen, Karla V Ballman, Lewis C Cantley, Giorgio Inghirami, Olivier Elemento, Robert Baiocchi, Selina Chen-Kiang, Sandro Belvedere, Hongwu Zheng, and Jihye Paik
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Immunology ,Cell Biology ,Hematology ,Biochemistry - Abstract
Mantle cell lymphoma (MCL) often has an adverse prognosis and despite aggressive multimodal treatment with conventional and targeted therapies, the median survival of MCL patients remains approximately 4 years. Thus, there is a significant unmet need to find novel targets and rational combination treatments. Targeting lineage vulnerabilities driven by specific transcription factors has been broadly confirmed as an effective intervention in many human cancers. To identify the transcription program dependency of MCL cells, we conducted an unbiased domain-focused CRISPR-Cas9 screening against a library of 8,750 sgRNAs targeting 1,434 transcription factors in MCL cell lines (JEKO1, MAVER1, UPN1, and CCMCL1). We identified Forkhead Box O1 (FOXO1), EBF1, PAX5, and IRF4 as 4 transcription factors that are specifically required for MCL survival and growth. Chromatin-immunoprecipitation and sequencing (ChIP-Seq) analysis further revealed that the four transcription factors act together to orchestrate B cell lineage transcriptional program and MCL cell survival. Despite its well-recognized role as a tumor suppressor, FOXO1 has been implicated as a lineage specific transcription factor involved in mature B cell development. Genetic studies revealed a critical role of FOXO1 in germinal center dark zone formation and lymphomagenesis, raising the possibility that FOXO1 acts as a master transcription factor for lineage survival transcription program of MCL. Indeed, hierarchical interaction analysis revealed that FOXO1 functions as a pioneer factor that facilitate the chromatin access of other B cell lineage transcription factors. We demonstrated that interaction of FOXO1 to its cognate motif stabilizes B cell transcription factor complex and supports MCL progression. Along this line, we show that enforced expression of FOXO1 in myeloid leukemia cells induces transdifferentiation and B-cell specific gene expression. Mechanistically, we demonstrate through tiling CRISPR scanning screen that forkhead DNA binding and c-terminal transactivation domains of FOXO1 are specifically required for the viability of MCL cells. Given our finding of FOXO1 as a lineage-specific oncogene in MCL, we next explored the possibility of developing FOXO1-tageted inhibitors. We screened a library of potential small molecule inhibitors of FOXO1 (Forkhead BioTherapeutics) and identified cpd10 as one of the most potent and selective FOXO1 inhibitors (IC 50=76 ×/÷ 1.7. nM) Through the CCMCL1 MCL-NSG preclinical model, we found that cpd10 (100 mg/kg/daily) was well tolerated without overt toxicities. Importantly, prolonged treatment induced a robust cytotoxic response of MCL cells and suppressed MCL progression in vivo. Altogether, our findings identified FOXO1 as a MCL lineage-survival oncogene that can be exploited as a therapeutic target of future drug development. Disclosures Cantley: Petra Pharmaceuticals: Research Funding; Agios Pharmaceuticals: Membership on an entity's Board of Directors or advisory committees. Elemento: Freenome: Consultancy, Other: Current equity holder in a privately-held company; Volastra Therapeutics: Consultancy, Other: Current equity holder, Research Funding; Champions Oncology: Consultancy; Janssen: Research Funding; Owkin: Consultancy, Other: Current equity holder; AstraZeneca: Research Funding; One Three Biotech: Consultancy, Other: Current equity holder; Eli Lilly: Research Funding; Johnson and Johnson: Research Funding. Baiocchi: Prelude Therapeutics: Consultancy; viracta: Consultancy, Current holder of stock options in a privately-held company; Codiak Biosciences: Research Funding; Atara Biotherapeutics: Consultancy. Belvedere: Forkhead BioTherapeutics: Current Employment. Paik: Forkhead BioTherapeutics: Research Funding.
- Published
- 2021
15. Virgibacillus kimchii sp. nov., a halophilic bacterium isolated from kimchi
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Min-Sung Kwon, Ja-Young Jang, Nam Hee Kim, Myung-Ji Seo, Mi-Young Shin, Hyo Kyeong Park, Hak Jong Choi, Young J. Oh, Ji Eun Lee, and Seul Ki Lim
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DNA, Bacterial ,0301 basic medicine ,030106 microbiology ,Brassica ,Sodium Chloride ,Applied Microbiology and Biotechnology ,Microbiology ,03 medical and health sciences ,chemistry.chemical_compound ,RNA, Ribosomal, 16S ,Virgibacillus ,Phylogeny ,Base Composition ,Bacillaceae ,biology ,Fatty Acids ,Gracilibacillus ,General Medicine ,biology.organism_classification ,16S ribosomal RNA ,Halophile ,Bacterial Typing Techniques ,030104 developmental biology ,chemistry ,Fermentation ,Peptidoglycan ,Fermented Foods ,Bacteria - Abstract
A novel halophilic bacterium, strain K7(T), was isolated from kimchi, a traditional Korean fermented food. The strain is Gram-positive, motile, and produces terminal endospores. The isolate is facultative aerobic and grows at salinities of 0.0-25.0% (w/v) NaCl (optimum 10-15% NaCl), pH 5.5-8.5 (optimum pH 7.0-7.5), and 15-42°C (optimum 37°C). The predominant isoprenoid quinone in the strain is menaquinone-7 and the peptidoglycan of the strain is meso-diaminopimelic acid. The major fatty acids of the strain are anteisio-C15:0, iso-C15:0, and, C16:0 (other components were < 10.0%), while the major polar lipids are diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine, and three unidentified lipids. A phylogenetic analysis of 16S rRNA gene sequence similarity showed that the isolated strain was a cluster of the genus Gracilibacillus. High levels of gene sequence similarity were observed between strain K7(T) and Gracilibacillus orientalis XH-63(T) (96.5%), and between the present strain and Gracilibacillus xinjiangensis (96.5%). The DNA G+C content of this strain is 37.7 mol%. Based on these findings, strain K7(T) is proposed as a novel species: Gracilibacillus kimchii sp. nov. The type strain is K7(T) (KACC 18669(T); JCM 31344(T)).
- Published
- 2017
16. ALS-linked mutant SOD1 proteins promote Aβ aggregates in ALS through direct interaction with Aβ
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Ja Young Jang, Hyungmin Cho, Hyangshuk Rhim, Seongman Kang, and Hye Yoon Park
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0301 basic medicine ,Genetically modified mouse ,Programmed cell death ,SOD1 ,Mutant ,Biophysics ,Apoptosis ,Mice, Transgenic ,Biology ,Biochemistry ,Pathogenesis ,Mice ,Structure-Activity Relationship ,03 medical and health sciences ,Superoxide Dismutase-1 ,medicine ,Animals ,Humans ,Amyotrophic lateral sclerosis ,Molecular Biology ,Mutant sod1 ,Neurons ,Amyloid beta-Peptides ,Binding Sites ,Amyotrophic Lateral Sclerosis ,Cell Biology ,medicine.disease ,Cell biology ,HEK293 Cells ,030104 developmental biology ,Intracellular ,Protein Binding - Abstract
Amyotrophic lateral sclerosis (ALS) is a neurodegenerative disease characterized by progressive degeneration of motor neurons. Aggregation of ALS-linked mutant Cu/Zn superoxide dismutase (SOD1) is a hallmark of a subset of familial ALS (fALS). Recently, intracellular amyloid-β (Aβ) is detected in motor neurons of both sporadic and familial ALS. We have previously shown that intracellular Aβ specifically interacts with G93A, an ALS-linked SOD1 mutant. However, little is known about the pathological and biological effect of this interaction in neurons. In this study, we have demonstrated that the Aβ-binding region is exposed on the SOD1 surface through the conformational changes due to misfolding of SOD1. Interestingly, we found that the intracellular aggregation of Aβ is enhanced through the direct interaction of Aβ with the Aβ-binding region exposed to misfolded SOD1. Ultimately, increased Aβ aggregation by this interaction promotes neuronal cell death. Consistent with this result, Aβ aggregates was three-fold higher in the brains of G93A transgenic mice than those of non Tg. Our study provides the first direct evidence that Aβ, an AD-linked factor, is associated to the pathogenesis of ALS and provides molecular clues to understand common aggregation mechanisms in the pathogenesis of neurodegenerative diseases. Furthermore, it will provide new insights into the development of therapeutic approaches for ALS.
- Published
- 2017
17. Optimization of Herbicidin A Production in Submerged Culture of Streptomyces scopuliridis M40
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Sang-Il Lee, Hyun Jung Gwak, Hae Woong Park, Hak Jong Choi, Chang-Jin Kim, Keon Jin Lee, Sanghyun Ha, Jung-Sub Choi, Jin-Cheol Kim, Hyeong Hwan Kim, Jong-Hee Lee, Tae-Woon Kim, and Ja-Young Jang
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0106 biological sciences ,Morphology (linguistics) ,biology ,Hypha ,Substrate (chemistry) ,chemistry.chemical_element ,General Medicine ,010402 general chemistry ,biology.organism_classification ,01 natural sciences ,Applied Microbiology and Biotechnology ,Streptomyces ,Nitrogen ,0104 chemical sciences ,chemistry ,010608 biotechnology ,Bioreactor ,Fermentation ,Food science ,Streptomyces scopuliridis ,Biotechnology - Abstract
Herbicidin A is a potent herbicide against dicotyledonous plants as well as an antibiotic against phytopathogens. In this study, fermentation parameters for herbicidin A production in submerged culture of Streptomyces scopuliridis M40 were investigated. The herbicidin A concentration varied with the C/N ratio. High C/N ratios (>4) resulted in a herbicidin A production of more than 900 mg/l, whereas maximally 600 mg/l was obtained at ratios between 1 and 3.5. In 5-L batch fermentation, there was a positive correlation between the oxygen uptake rate (OUR) and herbicidin A production. Once the OUR increased, the substrate consumption rate increased, leading to an increase in volumetric productivity. Mechanical shear force affected the hyphal morphology and OUR. When the medium value of hyphal size ranged from 150 to 180 μm, high volumetric production of herbicidin A was obtained with OUR values >137 mg O2/l·h. The highest herbicidin A concentration of 956.6 mg/l was obtained at 500 rpm, and coincided with the highest relative abundance of hyphae of 100-200 μm length and the highest OUR during cultivation. Based on a constant impeller tip speed, which affects hyphal morphology, herbicidin A production was successfully scaled up from a 5-L jar to a 500-L pilot vessel.
- Published
- 2017
18. The New Approach for Establishing the Cellular Response Guideline for Medical Applications of Argon-Plasma Jet: Mitochondria and Colorimetric Polydiacetylene as Innovative Parameters
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Seongman Kang, Eun Ha Choi, Young Joon Hong, Ja Young Jang, Hyo-Chang Lee, Hyangshuk Rhim, Min Kyung Nam, Seongho Jeon, Chin-Wook Chung, and Jong-Man Kim
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Materials science ,Argon ,Biomedical Engineering ,Plasma jet ,Analytical chemistry ,Pharmaceutical Science ,Medicine (miscellaneous) ,chemistry.chemical_element ,Bioengineering ,04 agricultural and veterinary sciences ,Mitochondrion ,Stress indicator ,040401 food science ,Mitochondrial morphology ,Mitochondrial fragmentation ,0404 agricultural biotechnology ,chemistry ,Direct exposure ,Biophysics ,General Materials Science - Abstract
Argon plasma jet (Ar-PJ) has been widely used in clinical medicine; however, the cellular effects of Ar-PJ therapy applying to living tissues have not been clarified yet. It is necessary to investigate cellular responses to Ar-PJ in establishing guidelines on the therapeutic use of Ar-PJ. Interestingly, in the Ar-PJ-treated cells, the fragmented mitochondria, a typical cellular stress indicator, were discovered even in the cells located in the live zones (1∼3 zones). Using microscopic measurements of the mitochondrial length, we found that the fragmented mitochondria were mainly in the zones 1 and 2, the closest to the direct exposure point of Ar-PJ. Whereas, the mitochondria in the zone 4 retained their lengths to normal. This quantitative measurement of mitochondrial morphology was combined with the color scores of the polymerizable supramolecular (PS) sensor in diagnostic categories. The results demonstrate that the mitochondrial length (0.98∼3.94 μm) is inversely proportional to the PS sensor color scores (87∼0) in the zones 1∼4. On the combination of these three diagnostic parameters, the effective range of Ar-PJ for cellular responses was determined: the zones 1∼3, the color scores 87∼12 and the mitochondrial lengths 0.98∼2.57 μm. Our study is the first demonstration of mitochondrial fragmentation in response to Ar-PJ and the first attempt to establish the diagnostic guideline for Ar-PJ therapies by combinations with biological, physical and chemical aspects. Thus, this study will make great advances in the field of bioplasma applications.
- Published
- 2017
19. Anti-atherosclerotic effects of perilla oil in rabbits fed a high-cholesterol diet
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Ja Young Jang, Da-Woom Seo, Young-Hwan Ban, Sung-Pyo Lee, Yun-Bae Kim, Ehn-Kyoung Choi, Kyungha Shin, Jieun Choi, Jung-Min Yon, Haiyu Guo, Tae-Su Kim, Yeseul Cha, and Eun-Suk An
- Subjects
medicine.medical_specialty ,Hypercholesterolemia ,030204 cardiovascular system & hematology ,Lipid peroxidation ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,Internal medicine ,medicine ,perilla oil ,lcsh:QH301-705.5 ,fatty liver ,lcsh:R5-920 ,Cholesterol ,Fatty liver ,Lipid metabolism ,lipid peroxidation ,medicine.disease ,Perilla oil ,Atheroma ,Endocrinology ,chemistry ,Biochemistry ,lcsh:Biology (General) ,Original Article ,lipids (amino acids, peptides, and proteins) ,Lovastatin ,atherosclerosis ,lcsh:Medicine (General) ,030217 neurology & neurosurgery ,Corn oil ,medicine.drug - Abstract
Anti-atherosclerosis effects of perilla oil were investigated, in comparison with lovastatin, in rabbits fed a high-cholesterol diet (HCD). Hypercholesterolemia was induced in rabbits by feeding the HCD containing 0.5% cholesterol and 1% corn oil, and perilla oil (0.1 or 0.3%) was added to the diet containing 0.5% cholesterol for 10 weeks. HCD greatly increased blood total cholesterol and low-density lipoproteins, and caused thick atheromatous plaques, covering 74% of the aortic wall. Hyper-cholesterolemia also induced lipid accumulation in the liver and kidneys, leading to lipid peroxidation. Perilla oil not only attenuated hypercholesterolemia and atheroma formation, but also reduced fat accumulation and lipid peroxidation in hepatic and renal tissues. The results indicate that perilla oil prevents atherosclerosis and fatty liver by controlling lipid metabolism, and that it could be the first choice oil to improve diet-induced metabolic syndrome.
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- 2016
20. Anti-inflammatory effect of egg white-chalcanthite and purple bamboo salts mixture on arthritis induced by monosodium iodoacetate in Sprague-Dawley rats
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Dong-Yoon Kim, Ja-Young Jang, Beom-Seok Han, Hyun-kyung Song, Hyun-Kyung Park, Eun-A Choi, and Tae Hee Lee
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0301 basic medicine ,Monosodium iodoacetate ,medicine.drug_class ,Arthritis ,Osteoarthritis ,engineering.material ,Pharmacology ,Anti-inflammatory ,Nitric oxide ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,medicine ,lcsh:QH301-705.5 ,030203 arthritis & rheumatology ,Chalcanthite ,lcsh:R5-920 ,Chemistry ,purple bamboo salts (PBS) ,medicine.disease ,egg white-chalcanthite (EC) ,030104 developmental biology ,Biochemistry ,Blood chemistry ,arthritis ,lcsh:Biology (General) ,engineering ,monosodium iodoacetate (MIA) ,Original Article ,lcsh:Medicine (General) ,Egg white - Abstract
The aim of this study is to investigate the potential of anti-osteoarthritis effects on egg white-chalcanthite (EC), purple bamboo salts (PBS), and a mixture of EC and PBS (EC+PBS). EC is a mixture of egg white and pulverized chalcanthite. PBS has been widely used as one of functional foods in Korea and shows unique features compared with common salt. Osteoarthritis was induced by intra-articular injection of monosodium iodoacetate (MIA, 4mg/kg bw) in Sprague-Dawley (SD) rats. Test substances were administered once daily for 6 weeks at doses of 10 mg EC, EC+100 mg PBS, EC+200 mg PBS before and after MIA injection. Each substance was assessed by blood chemistry parameters, and by serum cytokines including IL-1β and IL-6, and nitric oxide (NO) and prostaglandin-E2 (PGE2). Structural changes of articular cartilage were also evaluated by histopathological examination. As a result, body weight and blood chemistry parameter were not different in all experimental groups. EC+PBS mixture reduced the production of PGE2, NO, IL-1β, and IL-6. In histological grade of osteoarthritis, EC+PBS mixture had a tendency to ameliorate damage of articular cartilage induced by MIA in a dose-dependent manner. In conclusion, EC+PBS mixture was demonstrated to have a potential for anti-inflammatory effect against osteoarthritis induced by MIA in a dose-dependent manner.
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- 2016
21. Salicibibacter halophilus sp. nov., a moderately halophilic bacterium isolated from kimchi
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Young J. Oh, Hak Jong Choi, Ja-Young Jang, Seul Ki Lim, Min-Sung Kwon, Joon Yong Kim, and Hyo Kyeong Park
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DNA, Bacterial ,Diamino acid ,Peptidoglycan ,Sodium Chloride ,Diaminopimelic Acid ,Applied Microbiology and Biotechnology ,Microbiology ,Genome ,03 medical and health sciences ,chemistry.chemical_compound ,RNA, Ribosomal, 16S ,Republic of Korea ,Genome size ,Bacillaceae ,Phylogeny ,030304 developmental biology ,0303 health sciences ,Base Composition ,biology ,Phylogenetic tree ,Whole Genome Sequencing ,030306 microbiology ,Fatty Acids ,Vitamin K 2 ,General Medicine ,Genomics ,Salt Tolerance ,Sequence Analysis, DNA ,Hydrogen-Ion Concentration ,16S ribosomal RNA ,biology.organism_classification ,Halophile ,Bacterial Typing Techniques ,Halobacteriales ,chemistry ,Biochemistry ,Genes, Bacterial ,Fermented Foods ,Bacteria - Abstract
A Gram-stain-positive, rod-shaped, alkalitolerant, and halophilic bacterium–designated as strain NKC3-5T–was isolated from kimchi that was collected from the Geumsan area in the Republic of Korea. Cells of isolated strain NKC3-5T were 0.5–0.7 μm wide and 1.4–2.8 μm long. The strain NKC3-5T could grow at up to 20.0% (w/v) NaCl (optimum 10%), pH 6.5–10.0 (optimum pH 9.0), and 25–40°C (optimum 35°C). The cells were able to reduce nitrate under aerobic conditions, which is the first report in the genus Salicibibacter. The genome size and genomic G + C content of strain NKC3-5T were 3,754,174 bp and 45.9 mol%, respectively; it contained 3,630 coding sequences, 16S rRNA genes (six 16S, five 5S, and five 23S), and 59 tRNA genes. Phylogenetic analysis based on 16S rRNA showed that strain NKC3-5T clustered with bacterium Salicibibacter kimchii NKC1-1T, with a similarity of 96.2–97.6%, but formed a distinct branch with other published species of the family Bacillaceae. In addition, OrthoANI value between strain NKC3-5T and Salicibibacter kimchii NKC1-1T was far lower than the species demarcation threshold. Using functional genome annotation, the result found that carbohydrate, amino acid, and vitamin metabolism related genes were highly distributed in the genome of strain NKC3-5T. Comparative genomic analysis revealed that strain NKC3-5T had 716 pan-genome orthologous groups (POGs), dominated with carbohydrate metabolism. Phylogenomic analysis based on the concatenated core POGs revealed that strain NKC3-5T was closely related to Salicibibacter kimchii. The predominant polar lipids were phosphatidylglycerol and two unidentified lipids. Anteiso-C15:0, iso-C17:0, anteiso-C17:0, and iso-C15:0 were the major cellular fatty acids, and menaquinone-7 was the major isoprenoid quinone present in strain NKC3-5T. Cell wall peptidoglycan analysis of strain NKC3-5T showed that meso-diaminopimelic acid was the diagnostic diamino acid. The phephenotypic, genomic, phylogenetic, and chemotaxonomic properties reveal that the strain represents a novel species of the genus Salicibibacter, for which the name Salicibibacter halophilus sp. nov. is proposed, with the type strain NKC3-5T (= KACC 21230T = JCM 33437T).
- Published
- 2019
22. Human Placenta Hydrolysate Promotes Liver Regeneration via Activation of the Cytokine/Growth Factor-Mediated Pathway and Anti-oxidative Effect
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Tae Hee, Lee, Dong Sun, Park, Ja Young, Jang, Isaac, Lee, Jong Man, Kim, Gyu Seong, Choi, Chang Taek, Oh, Jeom Yong, Kim, Hae Jung, Han, Beom Seok, Han, and Jae Won, Joh
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Male ,Placenta ,Antioxidants ,Cell Line ,Liver Regeneration ,Rats, Sprague-Dawley ,Oxidative Stress ,Pregnancy ,Animals ,Hepatectomy ,Humans ,Intercellular Signaling Peptides and Proteins ,Female ,Signal Transduction - Abstract
Liver regeneration is a very complex process and is regulated by several cytokines and growth factors. It is also known that liver transplantation and the regeneration process cause massive oxidative stress, which interferes with liver regeneration. The placenta is known to contain various physiologically active ingredients such as cytokines, growth factors, and amino acids. In particular, human placenta hydrolysate (hPH) has been found to contain many amino acids. Most of the growth factors found in the placenta are known to be closely related to liver regeneration. Therefore, in this study, we investigated whether hPH is effective in promoting liver regeneration in rats undergoing partial hepatectomy. We confirmed that cell proliferation was significantly increased in HepG2 and human primary cells. Hepatocyte proliferation was also promoted in partial hepatectomized rats by hPH treatment. hPH increased liver regeneration rate, double nucleic cell ratio, mitotic cell ratio, proliferating cell nuclear antigen (PCNA), and K
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- 2019
23. Protective effect of soy powder and microencapsulation on freeze-dried Lactobacillus brevis WK12 and Lactococcus lactis WK11 during storage
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Hae Woong Park, Ja-Young Jang, Hak Jong Choi, Hyun Jung Gwak, Jong-Hee Lee, Tae-Woon Kim, and Sang-Il Lee
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food.ingredient ,biology ,Chemistry ,Lactobacillus brevis ,Lactococcus lactis ,biology.organism_classification ,Applied Microbiology and Biotechnology ,Trehalose ,Lactic acid ,chemistry.chemical_compound ,Freeze-drying ,food ,Skimmed milk ,Yeast extract ,Food science ,Bacteria ,Food Science ,Biotechnology - Abstract
Food-grade cryoprotective agents, i.e., skim milk, soy powder, yeast extract, and trehalose were tested for viability of lactic acid bacteria (LAB, Lactobacillus brevis WK12 and Lactococcus lactis WK11) during freeze drying. Ten percentage of soy powder showed a strong protective effect upon viability of both LAB, showing 1.85×1011 CFU/mL for L. brevis WK12 and 1.89×1011 CFU/mL for L. lactis WK11. Microencapsulation in Ca-alginate beads increased viability of LAB, compared to nonencapsulated cells. Moreover, there was a synergetic effect on the stability of LAB when encapsulated LAB in Ca-alginate beads were soaked in 10% of soy powder prior to freeze-drying. When temperature increased, however, survival of LAB decreased. Four weeks after storage the highest viability was observed at -18°C for L. brevis WK12 and L. lactis WK11, showing 1.80×1011 CFU/mL and 1.78×1011 CFU/mL, respectively. These results suggest that soy powder may be useful for enhancing viability of LAB with microencapsulation.
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- 2015
24. Lactic Acid Bacteria in Kimchi
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Jieun Lee, Ja-Young Jang, and Hak-Jong Choi
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chemistry.chemical_compound ,chemistry ,biology ,Food science ,biology.organism_classification ,Bacteria ,Lactic acid - Published
- 2018
25. The New Approach for Establishing the Cellular Response Guideline for Medical Applications of Polydiacetylene as Innovative Parameters
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Min-Kyung, Nam, Hyo-Chanag, Lee, Young Joon, Hong, Ja-Young, Jang, Eun Ha, Choi, Chin-Wook, Chung, Seongho, Jeon, Jong-Man, Kim, Seongman, Kang, and Hyangshuk, Rhim
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Mice, Inbred C57BL ,Mice ,Argon Plasma Coagulation ,Polymers ,Animals ,Polyynes ,Colorimetry ,Argon ,Cells, Cultured ,Polyacetylene Polymer ,Mitochondria - Abstract
Argon plasma jet (Ar-PJ) has been widely used in clinical medicine; however, the cellular effects of Ar-PJ therapy applying to living tissues have not been clarified yet. It is necessary to investigate cellular responses to Ar-PJ in establishing guidelines on the therapeutic use of Ar-PJ. Interestingly, in the Ar-PJ-treated cells, the fragmented mitochondria, a typical cellular stress indicator, were discovered even in the cells located in the live zones (1∼3 zones). Using microscopic measurements of the mitochondrial length, we found that the fragmented mitochondria were mainly in the zones 1 and 2, the closest to the direct exposure point of Ar-PJ. Whereas, the mitochondria in the zone 4 retained their lengths to normal. This quantitative measurement of mitochondrial morphology was combined with the color scores of the polymerizable supramolecular (PS) sensor in diagnostic categories. The results demonstrate that the mitochondrial length (0.98∼3.94 μm) is inversely proportional to the PS sensor color scores (87∼0) in the zones 1∼4. On the combination of these three diagnostic parameters, the effective range of Ar-PJ for cellular responses was determined: the zones 1∼3, the color scores 87∼12 and the mitochondrial lengths 0.98∼2.57 μm. Our study is the first demonstration of mitochondrial fragmentation in response to Ar-PJ and the first attempt to establish the diagnostic guideline for Ar-PJ therapies by combinations with biological, physical and chemical aspects. Thus, this study will make great advances in the field of bioplasma applications.
- Published
- 2018
26. Mixture of Extracts of Cynanchum wilfordii and Phlomis umbrosa Turcz. Does Not Have an Estrogenic Effect in Ovariectomized Rats
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Sang-Keun Hong, Tae Hee Lee, Song-Hee Han, Yu Ri Kim, Ja-Young Jang, Hyun-kyung Song, and Beom-Seok Han
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medicine.medical_specialty ,Traditional medicine ,Triglyceride ,Cholesterol ,medicine.drug_class ,Body weight ,Growth hormone ,Applied Microbiology and Biotechnology ,chemistry.chemical_compound ,Endocrinology ,chemistry ,Estrogen ,Internal medicine ,Cynanchum wilfordii ,medicine ,Ovariectomized rat ,Phlomis umbrosa ,Food Science ,Biotechnology - Abstract
Institute of Life Sciences & BiotechnologyAbstract Cynanchum wilfordii and Phlomis umbrosa Turcz. are known to contain isoflavone, a representative phytoestrogen.This study was performed to determine whether the extract mixture of C. wilfordii and P. umbrosa Turcz. would inducean estrogenic effect in ovariectomized rats. The extracts were administered to the ovariectomized rats at 30, 60, 120 mg/kg per day for 4 weeks, respectively. They showed no estrogenic effect, which was indicated by the decrease in uteruswall thickness as well as the increase in body weight and the level of cholesterol and triglyceride. The extracts also hadno effect on the concentrations of estrogen and growth hormone in the serum. However, the increase in alkalinephosphatase activity, which leads to protection against the bone loss caused by ovariectomy, was noted on administrationof the extract. Therefore, it seemed that the extracts of C. wilfordii and P. umbrosa Turcz. had no estrogenic effect in rats.Keywords: Cynanchum wilfordii, Phlomis umbrosa Turcz, isoflavone, estrogen, growth hormone ovariectomized rat
- Published
- 2015
27. A Dunnione Compound MB12662 Improves Cisplatin-Induced Tissue Injury and Emesis
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Dongsun Park, Jeong Hee Jeon, Yun-Bae Kim, Ehn-Kyoung Choi, Sang Ku Yoo, Seong Soo Joo, Tae Hwan Kwak, In Geun Jo, Ja Young Jang, and Okjin Kim
- Subjects
Pathology ,medicine.medical_specialty ,Pharmacology ,Biochemistry ,Nephrotoxicity ,Immune system ,Atrophy ,Drug Discovery ,medicine ,Splenocyte ,Retching ,Cisplatin ,business.industry ,MB12662 ,Emesis ,medicine.disease ,medicine.anatomical_structure ,Intestinal injury ,Vomiting ,Molecular Medicine ,Original Article ,Dunnione ,Bone marrow ,medicine.symptom ,business ,medicine.drug - Abstract
The present study was aimed to investigate the effects of MB12662, a synthetic dunnione compound, on cisplatin-induced vomiting reflexes and intestinal, renal, immune system, and hematopoietic toxicities in ferrets and mice, respectively. Male ICR mice were orally administered MB12662 (5, 10, 25 or 50 mg/kg) for 10 days, during which intraperitoneally challenged with cisplatin (3.5 mg/kg) from day 4 to 7, and sacrificed on day 10 for the pathological examination. Male ferrets were orally administered MB12662 (25, 50 or 100 mg/kg) for 7 days, subcutaneously challenged with cisplatin (5 mg/kg), and monitored for vomiting reflexes and survival of the animals. Four-day injection of cisplatin (3.5 mg/kg) to mice caused body weight loss and degeneration and atrophy of intestinal villi, reducing villi/crypt ratio to a half level of control animals. Cisplatin also induced renal and hepatic toxicities, and depletion of splenocytes and bone marrow progenitor cells. The systemic toxicities including decreased villi/crypt ratio, immune system atrophy, splenocyte depletion, and decreased cellularity in bone marrow were improved by MB12662. Cisplatin (5 mg/kg) induced retching and emetic responses of ferrets, which were remarkably attenuated by MB12662 in a dose-dependent manner. All the ferrets pretreated with MB12662 survived the challenge of cisplatin, in comparison with 40% mortality in vehicle-treated animals, and blood parameters of nephrotoxicity and hepatotoxicity were markedly recovered. It is expected that MB12662 could be a candidate for the body protection against burden, including emesis, of chemotherapeutic agents.
- Published
- 2015
28. High-pressure processing of milk alleviates atopic dermatitis in DNCB-induced Balb/c mice
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Young Jun Oh, Eun-Ji Choi, Jung Tae Jeon, Hyun Ju Kim, Jong-Hee Lee, Tae-Woon Kim, Sun Young Park, Ja-Young Jang, Jong Soo Kang, Ga Young Jeon, Hae Woong Park, Ji Eun Lee, Hak Jong Choi, Seul Ki Lim, and Min-Sung Kwon
- Subjects
0301 basic medicine ,biology ,business.industry ,food and beverages ,Hypoallergenic ,Atopic dermatitis ,biology.organism_classification ,medicine.disease ,Biochemistry ,Serum ige ,BALB/c ,Pascalization ,03 medical and health sciences ,030104 developmental biology ,Milk products ,High pressure ,Immunology ,Medicine ,business ,Food Science - Abstract
High pressure (HP) is recognized as the alternative process technology of heat treatment in food production. Although milk was the first food product to be treated with HP, the allergenicity of HP-treated milk remains to be elucidated. Thus, this study was conducted to investigate the effects of HP treatment on the alleviation of atopic dermatitis (AD) caused by milk in a mouse model. To investigate the effect of HP treatment on the alleviation of AD, Balb/c mice were fed HP-treated milk orally for 7 days following induction of AD with 1-chloro-2,4-dinitro-benzene (DNCB). Mice fed with HP-treated milk exhibited markedly lower serum IgE level than DNCB-treated mice. These results suggest that HP may be applicable as an alternative process for the development of hypoallergenic milk products.
- Published
- 2015
29. Extending the shelf life of kimchi with Lactococcus lactis strain as a starter culture
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Hak Jong Choi, Mo Eun Lee, Hae-Won Lee, Jong-Hee Lee, Tae-Woon Kim, Yu-Ryang Pyun, Ja-Young Jang, and Hae Woong Park
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education.field_of_study ,Strain (chemistry) ,Population ,Lactococcus lactis ,technology, industry, and agriculture ,food and beverages ,Biology ,Shelf life ,biology.organism_classification ,Applied Microbiology and Biotechnology ,Starter ,bacteria ,Fermentation ,Food science ,education ,Food Science ,Biotechnology - Abstract
Lactococcus lactis subsp. lactis WK11 was introduced as a starter in kimchi fermentation to investigate the role of starter cultures in extending the shelf life of kimchi. Kimchi, with an initial inoculum size of 107 CFU/g as well as control kimchi without the starter culture, were prepared and fermented for 56 days at 8°C. The addition of L. lactis prolonged the optimal fermentation period (pH 4.2, acidity 0.6–0.8%) up to more than 2 times. The starter culture, L. lactis WK11, persisted throughout the kimchi fermentation, as confirmed by regular monitoring of the L. lactis population by PCR-DGGE. Taken together, our results suggest that L. lactis starter culture may be useful for extending the shelf-life of kimchi without adverse effects of kimchi with a longer shelf life.
- Published
- 2015
30. Effect of Starter Cultures on the Antioxidant Activities of Allium hookeri Root-Hot Water Extract
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Young Jun Oh, Hak Jong Choi, Ja-Young Jang, Sung Hyun Lee, Hyun Ju Kim, Ji Eun Lee, and Eun-Ji Choi
- Subjects
Streptococcus thermophilus ,ABTS ,biology ,DPPH ,Allium hookeri ,food and beverages ,Phenolic acid ,biology.organism_classification ,Southeast asian ,chemistry.chemical_compound ,Horticulture ,Lactobacillus acidophilus ,Starter ,chemistry ,General Earth and Planetary Sciences ,General Environmental Science - Abstract
Allium hookeri, a member of the onion family, has long been mainly cultivated for food and medicinal use in Southeast Asian countries, owing to its various biological properties. However, no studies of the anti-oxidative effects of fermented A. hookeri root extracts have been conducted to date. Therefore, this study investigated the effect of different starter cultures on the antioxidant activities of hot water extract of A. hookeri root by using the following five strains: Lactobacillus acidophilus, Bifidobacterum longum, Enterococcus faecium, Streptococcus thermophilus, and Saccharomyces cerevisiae. DPPH and ABTS radical scavenging activity, total phenolic acid contents, and total antioxidant capacity were higher in the hot water extract of A. hookeri root fermented with starter cultures than those of A. hookeri root. Among hot water extract of A. hookeri root fermented with starter cultures, fementing with S. cerevisiae showed the highest antioxidant activities. The results of this study provide new evidence of the anti-oxidative properties of A. hookeri root with starter cultures, indicating that it may be highly valuable as a natural product owing to its high-quality functional components.
- Published
- 2015
31. Ethanol extracts of unroasted Coffea canephora robusta beans suppress adipogenesis in preadipocytes and fat accumulation in rats fed a high-fat diet
- Author
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Jingmei Cai, Sung-Pyo Lee, Youngeun Kim, Tae-Su Kim, Ehn-Kyoung Choi, Yun-Bae Kim, Jihyun Kim, Heon-Sang Jeong, Kyungha Shin, Ja Young Jang, and Jong-Choon Kim
- Subjects
medicine.medical_specialty ,biology ,Leptin ,Blood lipids ,medicine.disease ,Coffea canephora ,biology.organism_classification ,Applied Microbiology and Biotechnology ,Obesity ,Endocrinology ,Biochemistry ,In vivo ,Adipogenesis ,Internal medicine ,Hyperlipidemia ,medicine ,Steatosis ,Food Science ,Biotechnology - Abstract
Anti-adipogenic and anti-obesity effects of a green coffee been extract (GCE) from unroasted Coffea canephora Robusta beans were investigated in vitro and in vivo. Insulin-induced adipogenesis in 3T3-L1 preadipocytes was inhibited by 25.6 and 87.9% following treatment with 100 and 500 μg/mL GCE, respectively. Expression of adipogenesis-specific genes was down-regulated. Body weight gains of C57BL/6 mice fed a high-fat diet (HFD) were suppressed in a concentration-dependent manner using GCE (0.3 or 1% in the diet), exhibiting a marked lower gain in 1% GCE-fed mice than in normal diet-fed animals. Decreases in body fat weights and the size of adipocytes were confirmed. GCE supplementation reduced concentrations of blood lipids and leptin, and attenuated hepatic steatosis. GCE inhibits adipogenesis by modulating cell signaling and improves dietary obesity by reducing lipid accumulation and the size of adipocytes.
- Published
- 2014
32. Cold atmospheric plasma (CAP), a novel physicochemical source, induces neural differentiation through cross-talk between the specific RONS cascade and Trk/Ras/ERK signaling pathway
- Author
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Eun Ha Choi, Ja Young Jang, Jun Sup Lim, Jinsung Choi, Young June Hong, Hyangshuk Rhim, and Seongman Kang
- Subjects
0301 basic medicine ,Plasma Gases ,MAP Kinase Signaling System ,Biophysics ,Bioengineering ,Receptors, Nerve Growth Factor ,Mitochondrion ,Nitric Oxide ,Nitric oxide ,Biomaterials ,03 medical and health sciences ,chemistry.chemical_compound ,Mice ,Catecholamines ,Cytosol ,Neural Stem Cells ,Cell Line, Tumor ,Extracellular ,Animals ,Receptor ,Zebrafish ,Neurons ,Cell Differentiation ,Hydrogen Peroxide ,Reactive Nitrogen Species ,Cell biology ,Mitochondria ,030104 developmental biology ,chemistry ,Mechanics of Materials ,Trk receptor ,Ceramics and Composites ,ras Proteins ,Signal transduction ,Intracellular - Abstract
Plasma, formed by ionization of gas molecules or atoms, is the most abundant form of matter and consists of highly reactive physicochemical species. In the physics and chemistry fields, plasma has been extensively studied; however, the exact action mechanisms of plasma on biological systems, including cells and humans, are not well known. Recent evidence suggests that cold atmospheric plasma (CAP), which refers to plasma used in the biomedical field , may regulate diverse cellular processes, including neural differentiation. However, the mechanism by which these physicochemical signals, elicited by reactive oxygen and nitrogen species (RONS), are transmitted to biological system remains elusive. In this study, we elucidated the physicochemical and biological (PCB) connection between the CAP cascade and Trk/Ras/ERK signaling pathway , which resulted in neural differentiation. Excited atomic oxygen in the plasma phase led to the formation of RONS in the PCB network, which then interacted with reactive atoms in the extracellular liquid phase to form nitric oxide (NO). Production of large amounts of superoxide radical ( O2−) in the mitochondria of cells exposed to CAP demonstrated that extracellular NO induced the reversible inhibition of mitochondrial complex IV. We also demonstrated that cytosolic hydrogen peroxide, formed by O2− dismutation, act as an intracellular messenger to specifically activate the Trk/Ras/ERK signaling pathway. This study is the first to elucidate the mechanism linking physicochemical signals from the CAP cascade to the intracellular neural differentiation signaling pathway, providing physical, chemical and biological insights into the development of therapeutic techniques to treat neurological diseases.
- Published
- 2017
33. Optimization of Herbicidin A Production in Submerged Culture of
- Author
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Sanghyun, Ha, Keon Jin, Lee, Sang Il, Lee, Hyun Jung, Gwak, Jong-Hee, Lee, Tae-Woon, Kim, Hak-Jong, Choi, Ja-Young, Jang, Jung-Sub, Choi, Chang-Jin, Kim, Jin-Cheol, Kim, Hyeong Hwan, Kim, and Hae Woong, Park
- Subjects
Bacteriological Techniques ,Time Factors ,Rotation ,Nitrogen ,Cell Culture Techniques ,Hyphae ,Temperature ,Pilot Projects ,Purine Nucleosides ,Zea mays ,Carbon ,Streptomyces ,Culture Media ,Oxygen ,Bioreactors ,Glucose ,Batch Cell Culture Techniques ,Fermentation ,Soybeans - Abstract
Herbicidin A is a potent herbicide against dicotyledonous plants as well as an antibiotic against phytopathogens. In this study, fermentation parameters for herbicidin A production in submerged culture of
- Published
- 2017
34. Allium hookeri root extract exerts anti-inflammatory effects by nuclear factor-κB down-regulation in lipopolysaccharide-induced RAW264.7 cells
- Author
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Ye-Rang Yun, Bo-Ram You, Min Jung Lee, Jong-Sik Jin, Ja-Young Jang, Hyunju Kim, and Sung-Hyen Lee
- Subjects
Lipopolysaccharides ,0301 basic medicine ,Antioxidant ,Lipopolysaccharide ,medicine.drug_class ,medicine.medical_treatment ,Down-Regulation ,Nitric Oxide Synthase Type II ,Inflammation ,Pharmacology ,NF-κB ,Anti-inflammatory ,Allium ,Nitric oxide ,Mice ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,medicine ,Animals ,Allium hookeri ,chemistry.chemical_classification ,Reactive oxygen species ,biology ,Plant Extracts ,business.industry ,Anti-Inflammatory Agents, Non-Steroidal ,NF-kappa B ,General Medicine ,COX-2 ,biology.organism_classification ,iNOS ,Nitric oxide synthase ,RAW 264.7 Cells ,030104 developmental biology ,Complementary and alternative medicine ,chemistry ,Biochemistry ,Cyclooxygenase 2 ,030220 oncology & carcinogenesis ,biology.protein ,Erratum ,medicine.symptom ,business ,Research Article - Abstract
Background Allium hookeri (AH) is widely consumed as a vegetable and herbal medicine in southeastern Asia. AH has been reported antioxidant, antimicrobial, improvement of bone health and antidiabetic effects. In the present study, we investigated the inhibitory effect of a methanol extract of AH root (AHE) on inflammatory response in lipopolysaccharide (LPS)-induced RAW264.7 cells. Methods Initially, characterization of organic sulfur compounds in AHE was determined using high performance liquid chromatography-electrospray ionization-mass spectrometry (HPLC-ESI-MS). Cells were incubated with LPS and AHE for 24 h. The productions of nitric oxide (NO), reactive oxygen species (ROS), and inflammation-related cytokines were examined. Gene and protein expression of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) were assessed by polymerase chain reaction and Western blotting. Key factor, nuclear factor kappa B (NF-κB) was also determined. Results AHE contained organosulfur compounds such as alliin and S-allylcysteine by HPLC-ESI-MS. AHE significantly inhibited NO, ROS, and cytokines production in LPS-induced RAW264.7 cells. In addition, AHE treatment inhibited iNOS and COX-2 mRNA and protein levels, leading to a decrease in iNOS-derived NO level. Furthermore, NF-κB activation was, at least in part, suppressed by AHE treatment. Conclusion Our data suggest that AHE treatment inhibits the inflammation condition through suppression of iNOS and COX-2 expression via NF-κB down-regulation.
- Published
- 2017
35. Weissella cibaria WIKIM28 ameliorates atopic dermatitis-like skin lesions by inducing tolerogenic dendritic cells and regulatory T cells in BALB/c mice
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Hae Woong Park, Young J. Oh, Young-Do Nam, Seul Ki Lim, Myung-Ji Seo, Min-Sung Kwon, Ji Eun Lee, Seong Woon Roh, Ja-Young Jang, Jong-Hee Lee, and Hak Jong Choi
- Subjects
0301 basic medicine ,Administration, Oral ,Immunoglobulin E ,T-Lymphocytes, Regulatory ,Article ,Immune tolerance ,Dermatitis, Atopic ,03 medical and health sciences ,Bacterial Proteins ,Immune Tolerance ,Medicine ,Mesenteric lymph nodes ,Animals ,Immunologic Factors ,IL-2 receptor ,Weissella cibaria ,Skin ,Mice, Inbred BALB C ,Multidisciplinary ,biology ,business.industry ,FOXP3 ,Interleukin ,Atopic dermatitis ,Dendritic Cells ,medicine.disease ,biology.organism_classification ,Disease Models, Animal ,030104 developmental biology ,medicine.anatomical_structure ,Treatment Outcome ,Weissella ,Immunology ,biology.protein ,business - Abstract
The occurrence of atopic dermatitis (AD), a chronic inflammatory skin disease, has been increasing steadily in children and adults in recent decades. In this study, we evaluated the ability of the lactic acid bacterium Weissella cibaria WIKIM28 isolated from gatkimchi, a Korean fermented vegetable preparation made from mustard leaves, to suppress the development of AD induced by 2,4-dinitrochlorobenzene in a murine model. Oral administration of W. cibaria WIKIM28 reduced AD-like skin lesions, epidermal thickening, and serum immunoglobulin E levels. Furthermore, the production of type 2 helper T (Th2) cytokines such as interleukin (IL)-4, IL-5, and IL-13 decreased in peripheral lymph node cells. Moreover, the intake of W. cibaria WIKIM28 increased the proportion of CD4+CD25+Foxp3+ regulatory T (Treg) cells in mesenteric lymph nodes (MLNs) and IL-10 levels in polyclonally stimulated MLN cells. In conclusion, the oral administration of W. cibaria WIKIM28 isolated from gatkimchi ameliorated AD-like symptoms by suppressing allergic Th2 responses and inducing Treg responses. These results suggest that W. cibaria WIKIM28 may be applicable as a probiotic for the prevention and amelioration of AD.
- Published
- 2017
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36. Use of Food-Grade Protective Agents to Improve the Viability of Freeze-Dried Lactic Acid Bacteria
- Author
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Ja Young Jang, Hyun Jung Gwak, Na Ra Lee, Jong-Hee Lee, Tae-Woon Kim, Hae Woong Park, and Hak Jong Choi
- Subjects
chemistry.chemical_compound ,Freeze-drying ,chemistry ,biology ,Protective Agents ,Food grade ,biology.organism_classification ,Applied Microbiology and Biotechnology ,Bacteria ,Food Science ,Biotechnology ,Microbiology ,Lactic acid - Published
- 2014
37. Effects of High Pressure Treatment on the Microbiological and Chemical Properties of Milk
- Author
-
Hae Woong Park, Jung Tae Jeon, Young Jun Oh, Eun-Ji Choi, Sun Young Park, Ja-Young Jang, Hyunju Kim, Jong-Hee Lee, Tae-Woon Kim, Ji Eun Lee, Ga Young Jeon, Hak Jong Choi, and Seul Ki Lim
- Subjects
Chemistry ,High pressure ,Environmental chemistry ,Chemical property ,Applied Microbiology and Biotechnology ,Microbiology ,Biotechnology - Published
- 2014
38. Immunomodulatory Effects of Kimchi in Chinese Healthy College Students: A Randomized Controlled Trial
- Author
-
Mi Ae Lee, Ja-Young Jang, Ryowon Choue, Hansongyi Lee, and Do Yeon Kim
- Subjects
History ,medicine.medical_specialty ,Chinese ,Traditional medicine ,business.industry ,Anthropometry ,Computer Science Applications ,Education ,law.invention ,Immunomodulation ,Randomized controlled trial ,Kimchi ,law ,Internal medicine ,Fermentation ,Randomized Controlled Trial ,Medicine ,Original Article ,business ,Body mass index ,Lymphocyte subsets - Abstract
This study examined the potential immunomodulatory effects of Kimchi, a traditional fermented Korean vegetable, in healthy Chinese college students. The four-week clinical-trial (randomized, open-label, prospective, controlled) was followed by a one week wash-out period. Healthy Chinese college students (over 20 years of age with a body mass index of 18.5-23.0 kg/m 2 ) volunteered for this study. Forty-three students were randomly classified into two groups, Kimchi (n = 21, supplemented with 100 g of Kimchi per day) or non-Kimchi (n = 22, supplemented with 100 g of radish per day, control) groups. During the four-week intervention period, students were asked to maintain their usual diet and activity, and instructed not to take any medications, functional food products, or dietary supplements. Anthropometrics, nutritional intake, and blood immune parameters (lymphocyte subsets, cytokines, and immunoglobulins) were measured before and after the four weeks of intervention. Thirty-nine students (19 in the Kimchi group, 20 in the non-Kimchi group) finished the study. After the intervention, no significant changes were observed in lymphocyte subsets (T-cell, B-cell, NK cell), pro-inflammatory cytokines (IL-6, TNF- α), anti-inflammatory cy tokines (IL-4 and IL-10), and immunoglobulins (Ig A, G, and M) between groups in either the Kimchi or non-Kimchi. These results suggest that the short-term consumption of Kimchi has no immunomodulatory effects in healthy Chinese college students.
- Published
- 2014
39. Safety Evaluation of Heavy Metal in Salted Vegetable Foods from Diverse Origin in Korea
- Author
-
Tae-Woon Kim, Miran Kang, Ja-Young Jang, Sung-Hee Park, HaeWoong Park, Eung Soo Han, Hyunju Kim, Jong-Hee Lee, and Hak-Jong Choi
- Subjects
Metal ,Cadmium ,Materials science ,chemistry ,visual_art ,visual_art.visual_art_medium ,chemistry.chemical_element ,Food science - Abstract
This study was conducted to estimate the contents of heavy metals in salted vegetable foods fromdiverse origin in Korea which were sold in Korea. The levels of heavy metals were determined using an ICP-MS. Thevalues of metals [minimum~maximum (mean), mg/kg] in kimchi were as follows; Pb 0.0~0.074(0.018), Cd 0.0~0.027(0.004), As 0.0~0.024(0.002), Hg 0~0.002(0.0). The weekly average intakes of lead, cadmium, arsenic and mercuryfrom kimchi take 0.06~0.13% PTWI (Provisional Tolerable Weekly Intakes) that the FAO/WHO Joint Food Additiveand Contaminants Committee has set to evaluate their safeties. Key words : Safety, Fermented vegetable foods, lead, cadmium 중금속은 비중이 4 이상 되는 금속원소로서 86종 가량이 있으며, 이들 중 약 27종만이 생체의 기능유지에 필수적이며, 필요량에서 부족하거나 다량일 경우 건강상의 장해를 일으킨다. 대다수의 금속은 영양학적으로 무의미하며 그 중에서 동·식물체에 유해성이 큰 중금속으로는 수은(Hg), 카드뮴(Cd), 납(Pb), 비소(As), 주석(Sn), 아연(Zn),구리(Cu), 크롬(Cr), 망간(Mn) 등이 이에 해당한다. 이들금속의 농도, 존재하는 화학성, 배설 속도, 건강 상태 등다양한 인자에 따라 생체에서의 유해 여부에 차이가 있다. 그런데 중금속은 자신이 가지고 있는 독성뿐만 아니라 축적성이 있어 먹이 연쇄에 따라 크게 농축된다. 특히, 수은, 카드뮴, 납은 식품 중에서 공통적으로 볼 수 있는 독성 물질로 생체 조직과 강한 결합을 하여 생체 내에서 축적되어 천천히 제거되는 유해 금속이다
- Published
- 2014
40. Comparative effects of plant oils on the cerebral hemorrhage in stroke-prone spontaneously hypertensive rats
- Author
-
Sung-Pyo Lee, Dongsun Park, Ehn-Kyoung Choi, Ja-Young Jang, Junsoo Lee, Byeongwoo Ahn, Jingmei Cai, Tae-Su Kim, Kwang Sei Kim, Kyungha Shin, Jihyun Kim, and Yun-Bae Kim
- Subjects
Male ,0301 basic medicine ,Trans fat ,food.ingredient ,Medicine (miscellaneous) ,Physiology ,Infarction ,Hyperlipidemias ,030204 cardiovascular system & hematology ,Kidney ,Rats, Inbred WKY ,Random Allocation ,03 medical and health sciences ,0302 clinical medicine ,food ,Dietary Fats, Unsaturated ,Rats, Inbred SHR ,Fatty Acids, Omega-3 ,Convulsion ,Hyperlipidemia ,Animals ,Plant Oils ,Medicine ,Sodium Chloride, Dietary ,Canola ,Stroke ,Cerebral Hemorrhage ,Metabolic Syndrome ,Neurons ,Nutrition and Dietetics ,Platelet Count ,business.industry ,General Neuroscience ,Brain ,alpha-Linolenic Acid ,General Medicine ,medicine.disease ,Dietary Fats ,Survival Analysis ,Thrombocytopenia ,Perilla oil ,030104 developmental biology ,Anesthesia ,Hypertension ,Rapeseed Oil ,medicine.symptom ,Metabolic syndrome ,business - Abstract
Since oils and fats can induce metabolic syndrome, leading to cardiovascular and cerebrovascular diseases, the present study was performed to find out whether the plant oils affect the cerebral hemorrhage in stroke-prone spontaneously hypertensive (SHR-SP) rats.From 47 days of age, male SHR-SP rats were given drinking water containing 1% NaCl to induce hypertension, and simultaneously fed semi-purified diets containing 10% perilla oil, canola oil, or shortening. The onset time of convulsion following cerebral hemorrhage was recorded, and the areas of hemorrhage and infarction were analyzed in the stroke brains.In comparison with 58-day survival of SHR-SP rats during feeding NaCl alone, perilla oil extended the survival time to 68.5 days, whereas canola oil shortened it to 45.7 days. Feeding perilla oil greatly reduced the total volume of cerebral hemorrhage from 17.27% in the control group to 4.53%, while shortening increased the lesions to 21.23%. In a microscopic analysis, perilla oil also markedly decreased the hemorrhagic and infarction lesions to 1/10 of those in control rats, in contrast to an exacerbating effect of shortening. In blood analyses, perilla oil reduced blood total cholesterol and low-density lipoproteins which were increased in SHR-SP, but canola oil further increased them and markedly lowered platelet counts.Perilla oil delayed and attenuated cerebral hemorrhage by improving hyperlipidemia in hypertensive stroke animals, in contrast to the aggravating potential of canola oil and shortening. It is suggested that perilla oil should be the first choice oil for improving metabolic syndrome in hypertensive persons at risk of hemorrhagic stroke.
- Published
- 2014
41. Antibacterial Activity of Lactic Acid Bacteria Isolated from Kimchi
- Author
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Jong-Hee Lee, Sun Young Park, Hae Woong Park, Tae-Woon Kim, Hak-Jong Choi, and Ja Young Jang
- Subjects
chemistry.chemical_compound ,biology ,Chemistry ,General Medicine ,Food science ,Antibacterial activity ,biology.organism_classification ,Bacteria ,Lactic acid - Published
- 2014
42. Allium hookeri Root Extract Inhibits Adipogenesis by Promoting Lipolysis in High Fat Diet-Induced Obese Mice
- Author
-
Min Jung Lee, Hyunju Kim, Ja-Young Jang, and Sung-Hyen Lee
- Subjects
0301 basic medicine ,medicine.medical_specialty ,anti-obesity ,Adipose tissue ,lcsh:TX341-641 ,Hormone-sensitive lipase ,White adipose tissue ,Article ,adipogenesis ,03 medical and health sciences ,chemistry.chemical_compound ,Adipocyte ,Internal medicine ,medicine ,Lipolysis ,030109 nutrition & dietetics ,Nutrition and Dietetics ,Allium hookeri root ,biology ,high fat diet ,food and beverages ,Fatty acid synthase ,030104 developmental biology ,Endocrinology ,chemistry ,Adipogenesis ,Lipogenesis ,lipolysis ,biology.protein ,lipids (amino acids, peptides, and proteins) ,lcsh:Nutrition. Foods and food supply ,Food Science - Abstract
Allium hookeri (AH) is widely consumed as a herbal medicine. It possesses biological activity against metabolic diseases. The objective of this study was to investigate effects of AH root water extract (AHR) on adipogenesis in 3T3-L1 cells and in high-fat diet (HFD)-induced obese mice. AHR inhibited lipid accumulation during adipocyte differentiation by downregulation of gene expression, such as hormone sensitive lipase (HSL), lipoprotein lipase (LPL) and an adipogenic gene, CCAAT/enhancer binding protein-&alpha, in 3T3-L1 preadipocytes. Oral administration of AHR significantly suppressed body weight gain, adipose tissue weight, serum leptin levels, and adipocyte cell size in HFD-induced obese mice. Moreover, AHR significantly decreased hepatic mRNA expression levels of cholesterol synthesis genes, such as 3-hydroxy-3-methylglutaryl CoA reductase, sterol regulatory element-binding transcription factor (SREBP)-2, and low-density lipoprotein receptor, as well as fatty acid synthesis genes, such as SREBP-1c and fatty acid synthase. Serum triglyceride levels were also lowered by AHR, likely as a result of the upregulating gene involved in fatty acid &beta, oxidation, carnitine palmitoyltransferase 1a, in the liver. AHR treatment activated gene expression of peroxisome proliferator-activated receptor-&gamma, which might have promoted HSL and LPL-medicated lipolysis, thereby reducing white adipose tissue weight. In conclusion, AHR treatment can improve metabolic alterations induced by HFD in mice by modifying expression levels of genes involved in adipogenesis, lipogenesis, and lipolysis in the white adipose tissue and liver.
- Published
- 2019
43. Quality characteristics of kimchi with Allium hookeri root powder added
- Author
-
Eugene Kim, Ja-Young Jang, Hak Jong Choi, Bo Ram You, and Hyunju Kim
- Subjects
chemistry.chemical_classification ,Vitamin ,Taste ,Vitamin C ,biology ,Chemistry ,Allium hookeri ,biology.organism_classification ,Reducing sugar ,Salinity ,Horticulture ,chemistry.chemical_compound ,Fermentation ,Food science ,Quality characteristics ,Food Science - Abstract
This study was conducted to investigate the quality characteristics of kimchi with Allium hookeri powder (AHP) added during eight weeks fermentation at 4°C. AHP was added to salted cabbage at concentrations of 0, 0.1, 1, and 5% (w/w). The quality characteristics of the AHR-added kimchi were determined by measuring pH, acidity, salinity, reducing sugar, microbial amounts, and sensory properties. The vitamin B1 and vitamin C contents of the Allium hookeri root were 0.04 mg/100 g and 5.76 mg/100 g, respectively. As for the mineral contents, the K content was highest, followed by the Ca, Mg, and Fe contents. The pH was higher in the kimchi with 0.1%, 1%, and 5% AHP than in the kimchi without AHP during the eight-week fermentation. The salinity ranged from 2.02% to 2.37% over the eight weeks. The microbial cells and lactobacilli increased rapidly throughout the fermentation in the exponential phase and hardly increased in the stationary phase. In the sensory evaluations, the overall acceptance, taste, and texture of the kimchi with 1% AHP added were highest. In conclusion, the kimchi with 0.1%, 1%, and 5% AHP generally showed better quality than the kimchi without AHP. Especially, the kimchi with 1% AHP had the best scores in quality and overall acceptance during the fermentation.
- Published
- 2013
44. Mixture of TwoLactobacillus plantarumStrains Modulates the Gut Microbiota Structure and Regulatory T Cell Response in Diet-Induced Obese Mice
- Author
-
Min-Sung Kwon, Ji Eun Lee, Hyo Kyeong Park, Hee Eun Jo, Ja-Young Jang, Hak Jong Choi, Byung Hee Ryu, Young J. Oh, Misun Yun, Nam Hee Kim, Mi-Young Shin, Wooha Joo, Mi Yeon Ko, Seul Ki Lim, and Ji Hyun Lee
- Subjects
Male ,0301 basic medicine ,medicine.medical_specialty ,Regulatory T cell ,030209 endocrinology & metabolism ,Gut flora ,Weight Gain ,T-Lymphocytes, Regulatory ,Mice ,03 medical and health sciences ,0302 clinical medicine ,Downregulation and upregulation ,Immunity ,3T3-L1 Cells ,Internal medicine ,medicine ,Animals ,Obesity ,Triglycerides ,biology ,Probiotics ,Lipid Metabolism ,biology.organism_classification ,medicine.disease ,Gastrointestinal Microbiome ,Mice, Inbred C57BL ,Disease Models, Animal ,030104 developmental biology ,medicine.anatomical_structure ,Endocrinology ,Gene Expression Regulation ,Anti-Obesity Agents ,Diet-induced obese ,Akkermansia muciniphila ,Lactobacillus plantarum ,Food Science ,Biotechnology - Abstract
Scope The gut microbiota has been linked to diet-induced obesity, and microorganisms that influence obesity have important health implications. In this study, the anti-obesity effects of two Lactobacillus plantarum strains (DSR M2 and DSR 920) isolated from kimchi are investigated. Methods and results Mice are fed a normal or high-fat diet with or without DSR M2 and DSR 920 (DSR, 1 × 109 CFU d-1 ) for 12 weeks. DSR improves the obesity state, as evidenced by the i) suppressed obesity-related markers, e.g., gains in body weight and fat mass, ii) reduced serum and liver triglyceride levels, iii) upregulated β-oxidation and downregulated lipogenesis-related genes in the liver, iv) reduced serum leptin levels, v) altered microbial communities, vi) increased regulatory T cell immunity, and vii) suppressed inflammatory response. In addition, correlation analysis shows that Akkermansia muciniphila and the genus Anaerostipes, which are increased in the DSR group, are negatively correlated with obesity-related markers, but Mucispirillum schaedleri, which is increased in the high-fat-diet (HFD) group, is positively correlated with serum leptin level. Conclusion Lactobacillus plantarum DSR M2 and DSR 920 are candidate probiotics for the prevention and amelioration of obesity.
- Published
- 2018
45. Lactic Acid Bacteria in Kimchi and Their Immunomodulatory Activities
- Author
-
Tae-Woon Kim and Ja-Young Jang
- Subjects
chemistry.chemical_compound ,chemistry ,General Medicine ,Food science ,Biology ,biology.organism_classification ,Bacteria ,Lactic acid - Published
- 2013
46. Anti-obesity effects of glabridin-rich supercritical carbon dioxide extract of licorice in high-fat-fed obese mice
- Author
-
Ja-Young Jang, Tae-Youl Ha, Jiyun Ahn, Suna Kim, and Hyunjung Lee
- Subjects
Male ,medicine.medical_specialty ,Adipose Tissue, White ,Mice, Obese ,Adipose tissue ,White adipose tissue ,Diet, High-Fat ,Toxicology ,Mice ,chemistry.chemical_compound ,Carnitine palmitoyltransferase 1 ,Phenols ,Downregulation and upregulation ,3T3-L1 Cells ,Internal medicine ,Adipocytes ,CCAAT-Enhancer-Binding Protein-alpha ,Glycyrrhiza ,medicine ,Animals ,Adipogenesis ,Dose-Response Relationship, Drug ,biology ,Plant Extracts ,Chemistry ,Body Weight ,General Medicine ,Carbon Dioxide ,biology.organism_classification ,Isoflavones ,Mice, Inbred C57BL ,PPAR gamma ,Endocrinology ,Anti-Obesity Agents ,sense organs ,Phosphoenolpyruvate carboxykinase ,Glabridin ,Food Science - Abstract
Licorice (Glycyrrhiza glabra Linne) is a well-known medicinal plant and glabridin is an isoflavan isolated from licorice. In this study, we investigated the anti-obesity effect of glabridin and glabridin-rich supercritical fluid extract of licorice (LSC). Glabridin effectively inhibited adipogenesis in 3T3-L1 cells. Moreover, LSC showed inhibitory effect on adipogenesis in a dose-dependent manner. The inhibitory effect of LSC resulted from inhibiting the induction of the transcriptional factors CCAAT enhancer binding protein alpha and peroxisome proliferator-activated receptor gamma. Then we fed mice with high-fat diet containing none, 0.1% and 0.25% LSC for 8weeks to explore the anti-obesity effect of LSC in vivo. LSC significantly reduced weight gain by high-fat diet in a dose-dependent manner. The reductions of the hypertrophy of white adipose tissue and of fat cell size were also observed. In the liver, LSC supplementation effectively inhibited high-fat diet-induced hepatic steatosis through downregulation of gluconeogenesis related phosphoenolpyruvate carboxykinase and glucose 6-phosphatase and upregulation of the β-oxidation related carnitine palmitoyltransferase 1. Taken together, our results suggest that glabridin and glabridin-rich licorice extract would be effective anti-obesity agents.
- Published
- 2013
47. Gracilibacillus kimchii sp. nov., a halophilic bacterium isolated from kimchi
- Author
-
Young Joon Oh, Hae-Won Lee, Seul Ki Lim, Min-Sung Kwon, Jieun Lee, Ja-Young Jang, Hae Woong Park, Young-Do Nam, Myung-Ji Seo, and Hak-Jong Choi
- Subjects
0301 basic medicine ,Base Composition ,030106 microbiology ,Fatty Acids ,General Medicine ,Brassica ,Sodium Chloride ,Applied Microbiology and Biotechnology ,Microbiology ,03 medical and health sciences ,030104 developmental biology ,Fermentation ,Vegetables ,Bacillaceae ,Phylogeny - Abstract
A novel halophilic bacterium, strain K7(T), was isolated from kimchi, a traditional Korean fermented food. The strain is Gram-positive, motile, and produces terminal endospores. The isolate is facultative aerobic and grows at salinities of 0.0-25.0% (w/v) NaCl (optimum 10-15% NaCl), pH 5.5-8.5 (optimum pH 7.0-7.5), and 15-42°C (optimum 37°C). The predominant isoprenoid quinone in the strain is menaquinone-7 and the peptidoglycan of the strain is meso-diaminopimelic acid. The major fatty acids of the strain are anteisio-C15:0, iso-C15:0, and, C16:0 (other components were10.0%), while the major polar lipids are diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine, and three unidentified lipids. A phylogenetic analysis of 16S rRNA gene sequence similarity showed that the isolated strain was a cluster of the genus Gracilibacillus. High levels of gene sequence similarity were observed between strain K7(T) and Gracilibacillus orientalis XH-63(T) (96.5%), and between the present strain and Gracilibacillus xinjiangensis (96.5%). The DNA G+C content of this strain is 37.7 mol%. Based on these findings, strain K7(T) is proposed as a novel species: Gracilibacillus kimchii sp. nov. The type strain is K7(T) (KACC 18669(T); JCM 31344(T)).
- Published
- 2016
48. Kimchi suppresses 7-ketocholesterol-induced endoplasmic reticulum stress in macrophages
- Author
-
Miran Kang, Sung-Hee Park, Tae-Woon Kim, Hyun Ju Kim, Yeong-Ok Song, Yu Bin Sung, and Ja Young Jang
- Subjects
Chemistry ,ATF6 ,Endoplasmic reticulum ,Glutathione ,CHOP ,Applied Microbiology and Biotechnology ,Cell biology ,chemistry.chemical_compound ,Biochemistry ,Apoptosis ,Unfolded protein response ,Macrophage ,lipids (amino acids, peptides, and proteins) ,Cytotoxicity ,Food Science ,Biotechnology - Abstract
The oxidation of low-density lipoproteins (oxLDL) is considered as a key step in the development of atherosclerosis. Oxysterols such as 7-ketocholesterol (7-KC) have been reported to be partially responsible for the cytotoxicity of oxLDL and induce endoplasmic reticulum (ER) stress which eventually causes apoptosis. We aimed to investigate whether ER stress is induced by 7-KC and can be prevented by the kimchi methanol extract (KME) in RAW264.7 macrophage cells. ER stress induced by 7-KC was characterized by the activation of ER stress markers (GRP78, CHOP, and ATF6). ER stress could aggravate macrophage apoptosis, as assessed by caspase-3 activity. ER stress and apoptosis were inhibited by the KME and glutathione (GSH). We conclude that 7-KC causes aberrant ER stress and apoptosis, all of which are inhibited by KME and GSH. The inhibitory expression of ER stress markers highlights its new protective role against oxLDL-induced ER stress, apoptosis, and subsequent atherosclerosis.
- Published
- 2012
49. Anti-obesity effect of a standardised ethanol extract fromCurcuma longaL. fermented withAspergillus oryzaeinob/obmice and primary mouse adipocytes
- Author
-
Jin-Nyoung Ho, Ja Young Jang, Ho-Geun Yoon, Yongjae Kim, Sunoh Kim, Woojin Jun, and Jeongmin Lee
- Subjects
Nutrition and Dietetics ,Primary (chemistry) ,Ethanol ,biology ,Traditional medicine ,biology.organism_classification ,chemistry.chemical_compound ,Aspergillus oryzae ,chemistry ,Biochemistry ,Anti obesity ,Fermentation ,Curcuma ,Agronomy and Crop Science ,Food Science ,Biotechnology - Published
- 2012
50. Lentibacillus kimchii sp. nov., an extremely halophilic bacterium isolated from kimchi, a Korean fermented vegetable
- Author
-
Seul Ki Lim, Young-Do Nam, Hae Woong Park, Hae-Won Lee, Young Joon Oh, Myung-Ji Seo, Ji Eun Lee, Hak Jong Choi, Min-Sung Kwon, Jong-Hee Lee, Ja-Young Jang, and Seong Woon Roh
- Subjects
0301 basic medicine ,DNA, Bacterial ,Peptidoglycan ,Biology ,Sodium Chloride ,Diaminopimelic Acid ,Microbiology ,DNA, Ribosomal ,Cell wall ,03 medical and health sciences ,chemistry.chemical_compound ,Glycolipid ,Cell Wall ,RNA, Ribosomal, 16S ,Vegetables ,Molecular Biology ,Bacillaceae ,Phospholipids ,Phylogeny ,Oxidase test ,Fatty Acids ,Vitamin K 2 ,General Medicine ,Sequence Analysis, DNA ,16S ribosomal RNA ,biology.organism_classification ,Halophile ,Bacterial Typing Techniques ,Halobacteriales ,030104 developmental biology ,Phenotype ,chemistry ,Fermentation ,Food Microbiology ,Bacteria - Abstract
A Gram-positive, aerobic, non-motile and extremely halophilic bacterial strain, designated K9(T), was isolated from kimchi, a Korean fermented food. The strain was observed as endospore-forming rod-shaped cells showing oxidase and catalase activity. It was found to grow at 10.0-30.0 % (w/v) NaCl (optimum, 15.0-20.0 %), pH 7.0-8.0 (optimum, pH 7.5) and 15-40 °C (optimum, 30 °C). The polar lipids of strain K9(T) were identified as phosphatidylglycerol, three unidentified phospholipids and an unidentified glycolipid. The isoprenoid quinone was identified as menaquinone-7. The major cellular fatty acids (>20 % of the total) were found to be anteisio-C15:0 and anteisio-C17:0. The cell wall peptidoglycan composition was determined to contain meso-diaminopimelic acid. The G + C content of genomic DNA was determined to be 48.2 mol %. Phylogenetic analysis based on the 16S rRNA gene sequence revealed that the isolated strain is closely related to Lentibacillus salinarum AHS-1(T) (96.7 % sequence similarity). Based on its phenotypic, chemotaxonomic and phylogenetic data, strain K9(T) is considered to represent a novel species of the genus Lentibacillus, for which the name Lentibacillus kimchii sp. nov., is proposed. The type strain is K9(T) (=KACC 18490(T) = JCM 30234(T)).
- Published
- 2015
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