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7 results on '"JYOTHISHWARAN, G."'

2. A modified freeze–thaw method for efficient transformation of Agrobacterium tumefaciens

Author

Jyothishwaran, G, Kotresha, D, Selvaraj, T, Srideshikan, SH, Rajvanshi, PK, and Jayabaskaran, C

Subjects
fungi, food and beverages, Biochemistry
Abstract

There is a great potential for genetic manipulation of crop and medicinal plants to enhance productivity through increasing pest and microbial disease resistance and environmental stress tolerance, and also for studying gene function and regulation of physiological and developmental processes$^1$. Transgenic plants that transmit the introduced trait to progeny generations are produced using various DNA delivery methods such as particle acceleration$^2$, electroporation$^3$ and polyethylene glycol permeabilization of protoplasts$^4$. However, most commonly used method for obtaining transgenic plants is by the Agrobacterium tumefaciens-mediated transformation$^{5,6}$. Agrobacterium can transfer DNA to a remarkably broad group of organisms – numerous dicot and monocot angiosperm species$^5$, gymnosperms$^6$ and fungi, including yeast$^7$, ascomycetes$^8$ and basidiomycetes$^9$. Recently, Agrobacterium was reported to transfer DNA to human cells$^{10}$.

Published
2007

7. Antifertility effect of ethanolic extract of Amalakyadi churna in male albino mice.

Author

Seetharam YN, Sujeeth H, Jyothishwaran G, Barad A, Sharanabasappa G, Umareddy B, Vijaykumar MB, and Patil SB

Subjects
Animals, Complex Mixtures chemistry, DNA biosynthesis, Fruit chemistry, Male, Mice, Organ Size drug effects, Protein Biosynthesis, RNA biosynthesis, Sperm Count, Testis drug effects, Testis enzymology, Testis metabolism, Contraceptive Agents, Male pharmacology, Plant Extracts pharmacology
Abstract

Aim: To evaluate the antifertility activity of the ethanolic extract of Amalakyadi churna by oral administration in male albino mice., Methods: The ethanol extract of Amalakyadi churna at the dose of 250 mg/kg and 400 mg/kg body weight was administered orally for 30 days to adult male mice. On day 31, the mice were sacrificed and the testis and accessory reproductive organs were removed and weighed. The organs were processed for biochemical estimation and histological work., Results: Treatment with Amalakyadi churna resulted in decrease in the weights of testis and accessory reproductive organs. The diameters of testis, seminiferous tubules and Leydig cell nucleus were decreased. The spermatogenic elements, like spermatogonia, spermatocytes and spermatids in the testis were reduced significantly as well as the sperm count in cauda epididymis. There was a significant reduction in the protein, glycogen, DNA and RNA contents and the activity of acid phosphatase in the testis of extract treated mice compared with the control. The cholesterol content and the alkaline phophatase activity were increased significantly in treated mice., Conclusion: Amalakyadi churna extract arrests spermatogenesis in male mice without noticeable side effects.

Published
2003

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