1. Progress towards a reference genome for sunflower
- Author
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N.C. Kane, N. Gill, M.G. King, J.E. Bowers, H. Berges, J. Gouzy, E. Bachlava, N.B. Langlade, Z. Lai, M. Stewart, J.M. Burke, P. Vincourt, S.J. Knapp, L.H. Rieseberg, Dept Bot, University of British Columbia (UBC), Dept Plant Biol, University of Georgia [USA], Centre National de Ressources Génomiques Végétales (CNRGV), Institut National de la Recherche Agronomique (INRA), Unité mixte de recherche interactions plantes-microorganismes, Centre National de la Recherche Scientifique (CNRS)-Institut National de la Recherche Agronomique (INRA)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées, Monsanto Company, Indiana University, Indiana University System, Dept Biol, Indiana University [Bloomington], Indiana University System-Indiana University System, Genome Canada, Genome British Columbia, French National Institute for Agricultural Research (INRA), US National Science Foundation [0421630], and US Department of Agriculture [2008-35300-19263, 2008-35300-04579]
- Subjects
0106 biological sciences ,Compositae ,sunflower ,HELIANTHUS-ANNUUS L ,[SDV]Life Sciences [q-bio] ,Horticultural crops ,physical map ,Plant Science ,CULTIVATED SUNFLOWER ,Biology ,RFLP LINKAGE MAP ,SEQUENCE ,01 natural sciences ,DNA sequencing ,03 medical and health sciences ,Helianthus annuus ,Botany ,[SDV.BV]Life Sciences [q-bio]/Vegetal Biology ,reference genome ,Ecology, Evolution, Behavior and Systematics ,030304 developmental biology ,next generation sequencing ,2. Zero hunger ,0303 health sciences ,IDENTIFICATION ,Ecology ,MAIZE GENOME ,Noxious weed ,business.industry ,food and beverages ,Sunflower ,EVOLUTION ,WILD SUNFLOWERS ,Biotechnology ,ARABIDOPSIS-THALIANA ,business ,GENUS HELIANTHUS ,010606 plant biology & botany ,Reference genome - Abstract
International audience; The Compositae is one of the largest and most economically important families of flowering plants and includes a diverse array of food crops, horticultural crops, medicinals, and noxious weeds. Despite its size and economic importance, there is no reference genome sequence for the Compositae, which impedes research and improvement efforts. We report on progress toward sequencing the 3.5 Gb genome of cultivated sunflower (Helianthus annuus), the most important crop in the family. Our sequencing strategy combines whole-genome shotgun sequencing using the Solexa and 454 platforms with the generation of high-density genetic and physical maps that serve as scaffolds for the linear assembly of whole-genome shotgun sequences. The performance of this approach is enhanced by the construction of a sequence-based physical map, which provides unique sequence-based tags every 5-6 kb across the genome. Thus far, our physical map covers similar to 85% of the sunflower genome, and we have generated similar to 80x genome coverage with Solexa reads and 15.5x with 454 reads. Preliminary analyses indicated that similar to 78% of the sunflower genome consists of repetitive sequences. Nonetheless, similar to 76% of contigs >5 kb in size can be assigned to either the physical or genetic map or to both, suggesting that our approach is likely to deliver a highly accurate and contiguous reference genome for sunflower.
- Published
- 2011
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