Search

This study compares the fertility effects of inducing ovulation using the GnRH analogue, dephereline, versus natural GnRH at the end of a 5-day progesterone(P4)-based protocol for fixed-time artificial insemination (FTAI) in in heat-stressed and non-heat stressed lactating dairy cows. Cows were given GnRH (GnRH group, n = 369) or dephereline (DEPH group, n = 379) and were inseminated 14-20 h later. Dephereline treatment increased corpus luteum (CL) size on Day 7 post-AI compared with GnRH (P

9 páginas, 4 figuras, 1 tabla., Bovine besnoitiosis, caused by the apicomplexan Besnoitia besnoiti, is a chronic and debilitating disease characterized by cutaneous and systemic manifestations that primarily affects adult beef cattle. Previous studies have reported that clinical besnoitiosisis is rare in calves. However, we isolated B. besnoiti from a chronically infected calf for the first time. The identity of the Besnoitia species was determined after parasite isolation and molecular genotyping. According to the results obtained in vitro the new isolate, named as Bb-Spain3, was characterized in a reproducible in vitro model and was categorized as a low invader and low prolific isolate with a slower lytic cycle compared to Bb-Spain 1 isolate. Specific traits that differentiate isolates obtained from adult animals from those infecting calves were not found. Next, we described the first case report of chronic besnoitiosis in a female calf less than 6 months-old with a low body condition. The disease was confirmed by the presence of specific anti-B. besnoiti antibodies and parasite detection in the skin. At post-mortem examination, tissue samples were collected for histological, immunohistochemical and molecular analyses. DNA-parasite was detected in 31 different calf's tissues, being the most highly parasitized tissues the skin and the respiratory and reproductive tracts. In addition, the parasite was also present in heart, eyes, lymph nodes and brain. The high parasite load, a wide intra-organic parasite distribution and the presence of both viable and degenerated cysts, were indicative of a rapid progression of the disease. This case report underlines the need to include the inspection of young animals in besnoitiosis control., This study was financed by the Spanish Ministry of Economy and Competitiveness (AGL2013-46442-R), CYTED (The-matic Network 113RT0469 Protozoovac) and by the Community of Madrid (PLATESA S20137ABI-2906). Carlos Diezma-Díaz was financially supported through a grant from the Spanish Ministry of Economy and Competitiveness (BES-2014-069839), and Alejandro Jiménez-Meléndez was supported through a grant from the Spanish Ministry of Education, Culture and Sports (grant no. FPU 13/05481). M. Fernández is the recipient of a predoctoral contract from the “Junta de Castilla y León”, partially funded by the European Social Fund (European Union).We acknowledge the veterinarians Félix J. Díaz Gómez and Arturo Francisco González and the farmer for the help in samplings and data collection. Finally, we wish also to acknowledge Javier Moreno Gonzalo and Vanessa Navarro Lozano for their excellent technical assistance.