1. Nanoscale integration of single cell biologics discovery processes using optofluidic manipulation and monitoring
- Author
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Les P. Miranda, Ching Chen, Irwin Chen, Philip Jess, Aaron George Winters, Christopher Tan, Ming C. Wu, Philip Tagari, Fen-Fen Lin, Agi Hamburger, Jennitte Stevens, Marissa Mock, J. Tanner Nevill, Trent P. Munro, Su Chong, Kim Le, Marsela Jorgolli, and Han Xu
- Subjects
0106 biological sciences ,0301 basic medicine ,Process (engineering) ,advanced biotechnology ,Bioengineering ,Review ,01 natural sciences ,Applied Microbiology and Biotechnology ,drug discovery ,Automation ,03 medical and health sciences ,Lab-On-A-Chip Devices ,010608 biotechnology ,Humans ,Bioprocess ,digital cell biology ,Biological Products ,nanoscale cell culture ,single cell technology ,Bioprocess Engineering and Supporting Technologies ,Drug discovery ,business.industry ,optical manipulation techniques ,Data science ,Therapeutic modalities ,030104 developmental biology ,Workflow ,Science research ,Analytics ,bioassay development ,business ,Biotechnology - Abstract
The new and rapid advancement in the complexity of biologics drug discovery has been driven by a deeper understanding of biological systems combined with innovative new therapeutic modalities, paving the way to breakthrough therapies for previously intractable diseases. These exciting times in biomedical innovation require the development of novel technologies to facilitate the sophisticated, multifaceted, high‐paced workflows necessary to support modern large molecule drug discovery. A high‐level aspiration is a true integration of “lab‐on‐a‐chip” methods that vastly miniaturize cellulmical experiments could transform the speed, cost, and success of multiple workstreams in biologics development. Several microscale bioprocess technologies have been established that incrementally address these needs, yet each is inflexibly designed for a very specific process thus limiting an integrated holistic application. A more fully integrated nanoscale approach that incorporates manipulation, culture, analytics, and traceable digital record keeping of thousands of single cells in a relevant nanoenvironment would be a transformative technology capable of keeping pace with today's rapid and complex drug discovery demands. The recent advent of optical manipulation of cells using light‐induced electrokinetics with micro‐ and nanoscale cell culture is poised to revolutionize both fundamental and applied biological research. In this review, we summarize the current state of the art for optical manipulation techniques and discuss emerging biological applications of this technology. In particular, we focus on promising prospects for drug discovery workflows, including antibody discovery, bioassay development, antibody engineering, and cell line development, which are enabled by the automation and industrialization of an integrated optoelectronic single‐cell manipulation and culture platform. Continued development of such platforms will be well positioned to overcome many of the challenges currently associated with fragmented, low‐throughput bioprocess workflows in biopharma and life science research.
- Published
- 2019