1. Identification and characterisation of two runx2 homologues in zebrafish with different expression patterns
- Author
-
J. Samallo, T. van der Meulen, H. Schipper, J.L. van Leeuwen, Sander Kranenbarg, and H.G.J.M. Franssen
- Subjects
Male ,Bone Regeneration ,nervous-system ,repression domain ,Molecular Sequence Data ,Biophysics ,Pair-rule gene ,Biology ,Biochemistry ,Genome ,Structural Biology ,Gene duplication ,Gene cluster ,Genetics ,Animals ,Protein Isoforms ,Laboratorium voor Moleculaire Biologie ,Gene family ,Amino Acid Sequence ,Experimental Zoology ,Gene ,Zebrafish ,transcription factor ,Regulation of gene expression ,cbfa1 ,embryonic-development ,targeting signal ,Gene Expression Regulation, Developmental ,Genetic Variation ,Zebrafish Proteins ,biology.organism_classification ,gene-expression ,cleidocranial dysplasia ,Experimentele Zoologie ,osteoblast differentiation ,WIAS ,Female ,Laboratory of Molecular Biology ,acute myeloid-leukemia ,5' Untranslated Regions ,Transcription Factors - Abstract
Genome and gene duplications are considered to be the impetus to generate new genes, as the presence of multiple copies of a gene allows for paralogues to adopt novel function. After at least two rounds of genome/gene duplication, the Runt gene family consists of three members in vertebrates, instead of one in invertebrates. One of the family members, Runx2, plays a key role in the development of bone, a tissue that first occurs in vertebrates. The family has thus gained new gene function in the course of evolution. Two Runx2 genes were cloned in the vertebrate model system the zebrafish (Danio rerio). The expression patterns of the two genes differ and their kinetics differ up to four fold. In addition, splice forms exist that are novel when compared with mammals. Together, these findings comprise opportunities for selection and retention of the paralogues towards divergent and possibly new function. more...
- Published
- 2005
- Full Text
- View/download PDF