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The sterile insect technique (SIT) involves the mass release of sterile males to suppress insect pest populations. SIT has been improved for larval pests by the development of strains for female-specific tetracycline-suppressible (Tet-off) embryonic lethal systems for male-only populations. Here we describe the extension of this approach to the Mexican fruit fly, Anastrepha ludens, using a Tet-off driver construct with the Tet-transactivator (tTA) under embryo-specific Anastrepha suspensa serendipity α (As-sry-α) promoter regulation. In the absence of tetracycline, tTA acts upon a Tet-response element linked to the pro-apoptotic cell death gene lethal effector, head involuation defective (hid), from A. ludens (Alhid(Ala2) ) that contains a sex-specific intron splicing cassette, resulting in female-specific expression of the lethal effector. Parental adults double-homozygous for the driver/effector vectors were expected to yield male-only progeny when reared on Tet-free diet, but a complete lack of oviposited eggs resulted for each of the three strains tested. Ovary dissection revealed nonvitellogenic oocytes in all strains that was reversible by feeding females tetracycline for 5 days after eclosion, resulting in male-only adults in one strain. Presumably the sry-α promoter exhibits prezygotic maternal expression as well as zygotic embryonic expression in A. ludens, resulting in a Tet-off sterility effect in addition to female-specific lethality.

La investigacion se ejecuto en la Finca Experimental “La Maria” propiedad de la Universidad Tecnica Estatal de Quevedo (UTEQ) localizada en el km 7 1 / 2 de la via Quevedo-Mocache; Provincia de Los Rios cuya ubicacion geografica de 1o 6’ 23” de latitud sur y 79o 29’ 12” de longitud oeste y a una altura de 73 msnm. El objetivo principal fue evaluar el comportamiento productivo con la inclusion del 20% de harinas arbustivas y arboreas tropicales. Se utilizaron 40 cuyes machos de 30 dias de edad. Se empleo un diseno completamente al azar con cinco tratamientos, cuatro replicas y la unidad experimental estuvo conformado por 2 cuyes. Para determinar las diferencias entre medias de tratamientos se aplico la prueba de Tukey (P≤0,05). Se evaluaron cinco tratamientos en dietas de: (T0) dieta 100% balanceado, (T1) 80% dieta y 20% harina de Morus alba , (T2) 80% dieta y 20% harina de Erythrina poeppigiana , (T3) 80% dieta y 20% harina de Tithonia diversifolia , (T4) 80% dieta y 20% harina de Hibiscus rosa-sinensis . Las variables bajo estudio fueron: Consumo de alimento de balanceado en materia seca g (CABMS), ganancia de peso g (GP), indice de conversion alimenticia (ICA) y rendimiento a la canal % (RC). La rentabilidad de los tratamientos se la determino a traves de la relacion beneficio-costo (R b/c). El mayor (P

The sterile insect technique (SIT) involves the mass release of sterile males to suppress insect pest populations. SIT has been improved for larval pests by the development of strains for female-specific tetracycline-suppressible (Tet-off) embryonic lethal systems for male-only populations. Here we describe the extension of this approach to the Mexican fruit fly, Anastrepha ludens, using a Tet-off driver construct with the Tet-transactivator (tTA) under embryo-specific Anastrepha suspensa serendipity α (As-sry-α) promoter regulation. In the absence of tetracycline, tTA acts upon a Tet-response element linked to the pro-apoptotic cell death gene lethal effector, head involuation defective (hid), from A. ludens (Alhid(Ala2) ) that contains a sex-specific intron splicing cassette, resulting in female-specific expression of the lethal effector. Parental adults double-homozygous for the driver/effector vectors were expected to yield male-only progeny when reared on Tet-free diet, but a complete lack of oviposited eggs resulted for each of the three strains tested. Ovary dissection revealed nonvitellogenic oocytes in all strains that was reversible by feeding females tetracycline for 5 days after eclosion, resulting in male-only adults in one strain. Presumably the sry-α promoter exhibits prezygotic maternal expression as well as zygotic embryonic expression in A. ludens, resulting in a Tet-off sterility effect in addition to female-specific lethality.

The release of sterile males to suppress the Mexican fruit fly Anastrepha ludens (Loew) population was simulated in field cages to compare the sexual performance of sterile males with wild females in the presence and absence of sterile females. Using different release ratios of sterile and wild individuals, this study showed that the presence of sterile females negatively affected the sexual performance of sterile males in mating with wild females. We found that the sterility induced was proportional to the magnitude of the sterile/fertile ratio. These results indicate that the release of sterile males without sterile females can significantly improve the efficiency of the sterile insect technique for the control of A. ludens.

Results of a study of the inheritance and linkage relationships of eye-color mutations in the Mexican fruit fly, Anastrepha ludens (Loew) (Diptera: Tephritidae), are presented. The mutations white eyes (we) and yellow eyes (ye) are recessive and autosomal, whereas Red eyes (Re) and Violet eyes (Ve) are dominant, and the latter is homozygous lethal. The genes we and ye showed epistatic interaction, the first with ye, Re, and Ve, and the second with Re. The results of the various crosses suggest that only Ve and ye are linked, so the linkage groups have been designated as A- we, B- Ve-ye, and C- Re. Study of these mutants in A. ludens will serve as the basis for the development of a genetic map for this species. The mutations also will be useful for the development of a genetic-sexing method.