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1. Otitis Media

Author

Teralandur K. Parthasarathy and Gita J. Malur

Subjects
0303 health sciences, 03 medical and health sciences, 0302 clinical medicine, 030306 microbiology, 030225 pediatrics
Published
2005
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5. Quantitative reconstitution of isolated influenza haemagglutinin into liposomes by the detergent method and the immunogenicity of haemagglutinin liposomes

Author

R, Stahn, H, Schäfer, M, Kunze, J, Malur, A, Ladhoff, and U, Lachmann

Subjects
Mice, Mice, Inbred ICR, Detergents, Liposomes, Animals, Hemagglutinins, Viral, Proteins, Orthomyxoviridae, Lipids
Abstract

The reconstitution of influenza virus haemagglutinin into liposomes from lipid/protein/detergent mixtures by detergent removal provides vesicles that are similar in structure to viral particles. The dissociation properties of haemagglutinin aggregates and the molar ratio of lipid to protein in the starting mixture are the key factors for the individual and total yield of protein incorporation into liposomes. Structural properties of the detergent used as well as special reconstitution conditions are of minor importance for the formation of haemagglutinin liposomes. As determined by radial immunodiffusion-, haemolysis- and fusion experiments, specific properties of haemagglutinin were maintained to a large extent on liposomal incorporation, but its immunogenicity is increased, if the antigen is incorporated into the lipid bilayer of liposomes.

Published
1992

6. Aggregation of influenza virus haemagglutinin in the presence of detergents

Author

R, Stahn, H, Schäfer, J, Malur, and A, Ladhoff

Subjects
Chromatography, Protein Conformation, Detergents, Centrifugation, Density Gradient, Animals, Hemagglutinins, Viral, Hemagglutinin Glycoproteins, Influenza Virus, Orthomyxoviridae, Chickens
Abstract

In the haemagglutinin (HA) detergent mixtures coexist various protein complexes. Using 125I-HA tracer, gel chromatography, and centrifugation techniques we found protein aggregates comprising up to eight HA trimers. Formation of these structures seemed to be a function of the protein storage medium only. By contrast, special properties of the detergent as well as physicochemical conditions during the protein/detergent interaction had nearly no influence.

Published
1991

7. Otitis media

Author

Teralandur K. Parthasarathy, Gita J. Malur, and Vishwa Bhat

Subjects
03 medical and health sciences, Speech and Hearing, 0302 clinical medicine, Otitis, business.industry, 030225 pediatrics, Medicine, 030212 general & internal medicine, medicine.symptom, business, Primer (cosmetics), Microbiology
Published
2003
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8. Preparation and Properties of 125I-Hemagglutinin from Influenza Viruses. Iodination of a Membrane Glycoprotein

Author

H. Schäfer, R. Stahn, G. Petzold, and J. Malur

Subjects
chemistry.chemical_classification, Enzyme, chemistry, Biochemistry, Biophysics, Halogenation, Molecule, General Medicine, Carbohydrate, Hemagglutinin, Glycoprotein, Cleavage (embryo), Micelle
Abstract

Radio-iodination of influenza-hemagglutinin is proceeding with low yields only. It is demonstrated that the reason is not chemical of nature – impurities and/or the reducing properties of the carbohydrate substituents of hemagglutinin itself – but is due to the physical structure of the protein. By enzymatic cleavage and SDS-PAGE of 125I-hemagglutinin information is derived concerning the distribution of 125I-atoms in the tracer molecule. It is demonstrated, that results of this kind are sensibly monitoring differences of the chemical and/or physical properties of the preparations used for iodination. Furthermore, evidence is presented that hemagglutinin aggregation to micelles is without effect on iodination, but has serious implications generally on tracer experiments because of their high kinetic stability.

Published
1987
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9. Analyse von Materialien der Industrie, des Handels und der Landwirtschaft

Author

A. Sylvester, D. Klockow, A. Roşcovanu, M. Mengel, J. Malur, E. A. Dewaid, K. H. Neeb, M. Matucha, H. Sorantin, L. Johannsen, K. Henning, H. Pohl, W. Sternik, H. Bauer, E. Hradský, A. Schmidt, H. A. Rohleder, A. Niemann, B. R. Glutz, H. G. Wahrenholz, B. Ernst, H. Schwarz, M. Přibyl, M. Olivier, A. Dewald, and K. Wünscher

Subjects
Clinical Biochemistry, General Materials Science, General Medicine, Biochemistry, Analytical Chemistry
Published
1968
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10. Analyse von biologischem Material

Author

E. Wiegand, A. Niemann, W. Sternik, E. Müller, P. Haas, J. Malur, W. Czysz, H. Sorantin, M. Přibyl, K. Henning, K. Baranowsky, U. Haevecker, M. Müller, P. Buehler, H. Winkler, B. R. Glutz, A. E. Bühler, H. Bieling, A. Roşcovanu, M. Olivier, P. Gerhards, H. Bende, U. Gerhardt, A. Emr, G. Peters, J. Gasparič, G. Krall, F. Enzmann, and I. Peitzer

Subjects
Clinical Biochemistry, General Materials Science, General Medicine, Biochemistry, Analytical Chemistry
Published
1968
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11. Referate

Author

W. Czysz, H. Pohl, K. H. Neeb, M. Pavlova, P. Haas, B. Tvaroha, K. Henning, H. Wagner, H. Berge, I. Havezov, M. Olivier, K. H. Birr, H. A. Rohleder, R. Winkler, M. Hřivnáč, M. Müller, H. Hoffmann, H. -M. Lüschow, B. Tvarohs, A. Schurcidt, H. G. Warenholz, H. Sorantin, C. Keller, G. Schiewe, H. Pohn, L. Johannsen, J. Malur, P. Gerhards, N. Piperov, M. Oliver, D. Klockow, P. Bühler, F. Jančik, B. R. Glutz, H. G. Wahrenholz, L. Sommer, N. Krasnobaeva, A. Sylvester, M. Matucha, I. Pfitzer, H. Monien, W. Sternik, A. Niemann, M. Meyer zur Heyde, G. Peters, E. Müller, and Z. Stejskal

Subjects
Clinical Biochemistry, General Materials Science, General Medicine, Biochemistry, Analytical Chemistry
Published
1969
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12. Zur Bestimmung des Chloridgehalts von Zinksulfidluminophoren

Author

J. Malur

Subjects
Chemistry, Clinical Biochemistry, General Materials Science, General Medicine, Analytical Chemistry, Nuclear chemistry
Abstract

Zur Bestimmung chloridischer Verunreinigungen in Zinksulfidluminophoren wird eine potentiometrische Titrationsmethode vorgeschlagen. Verluste bei der Auflosung der Proben werden durch die Verwendung einer geschlossenen Apparatur vermieden. Die untere Erfassungsgrenze liegt bei etwa 10−5 g Cl/g ZnS. Die Chloridgehalte von ZnS-Luminophoren, welche mit chloridischen Mineralisatoren hergestellt wurden, ergaben sich zu 10−5 bis 2,5 · 10−4 g Cl/g ZnS. Eine Diskussion der Ergebnisse ist an anderer Stelle vorgesehen.

Published
1965
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13. Untersuchungen über die Grenzen der potentiometrischen Simultanbestimmung von Zink und Cadmium

Author

J. Malur and H. Treptow

Subjects
Chemistry, Clinical Biochemistry, General Materials Science, General Medicine, Analytical Chemistry, Nuclear chemistry
Abstract

Die Methode nach Agasjan1 zur potentiometrischen Simultanbestimmung von Zink und Cadmium mit Kaliumhexacyanoferrat(II) wurde uberarbeitet und auf ihre Anwendbarkeit zur Bestimmung kleiner Mengen der einen neben gro\en Mengen der anderen Ionenart gepruft. Diese Methode, die vor allem fur die Bestimmung der MengenverhAltnisse in Zinkcadmiumsulfid-Luminophoren, aber auch fur Ahnliche Probleme herangezogen werden kann, liefert gute Ergebnisse in den geschilderten Variationen, wobei die Metalle in Form ihrer Chloride, Sulfate oder Nitrate vorliegen sollen.

Published
1961
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14. Qualitative und quantitative Analyse

Author

K. Behrends, K. Henning, M. Přibyl, F. Jančik, A. Emr, K. H. Birr, H. Blaha, D. Rittweger-Heiligmann, P. Haas, B. R. Glutz, B. Rossmann, M. Meyer Zur Heyde, E. Dewald, J. Malur, B. Ernst, M. Bartušek, A. E. Bühler, M. Müller, A. Roşcovanu, P. Gerhards, and H. Winkler

Subjects
Clinical Biochemistry, General Materials Science, General Medicine, Biochemistry, Analytical Chemistry
Published
1968
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15. I. Allgemeine analytische Methoden, Apparate und Reagentien

Author

W. Schmidt, W. Sternik, D. Ceauşescu, H. Wagner, J. Malur, H. Berge, K. Bormann, B. R. Glutz, M. Olivier, M. Müller, D. Klockow, H. Monien, H. G. Eulenhöeer, H. Sorantin, G. Schiewe, K. Maas, L. Johannsen, K. Henning, and L. Sommer

Subjects
Clinical Biochemistry, General Materials Science, General Medicine, Biochemistry, Analytical Chemistry
Published
1968
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16. 3. Analysenmethoden auf dem Gebiete der Pharmazie

Author

K. Baranowsky, M. Matucha, J. Rasch, M. PŘibyl, B. Rossmann, A. Dewald, D. Rittweger-Heiligmann, H. Winkler, W. Czysz, K. Henning, Z. Stejskal, J. Malur, G. Schiewe, L. Johannsen, E. Hradský, H. Wagner, M. Müller, A. E. Bühler, E. Müller, P. Gerhards, M. Heyde, M. Mengel, B. Tvaroha, F. Jančik, and U. Gerhardt

Subjects
Clinical Biochemistry, General Materials Science, General Medicine, Biochemistry, Analytical Chemistry
Published
1968
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17. I. Allgemeine analytische Methoden, Apparate und Reagentien

Author

O. Fuchs, H. M. Lüschow, K. H. Neeb, M. Bartušek, J. Plank, W. Sternik, K. Henning, H. Schwarz, L. Sommer, M. Oliver, L. J. Ottendorfer, E. Dittrich, P. Haas, M. Meyer Zur Heyde, W. Czysz, M. Michlmayr, J. Malur, H. Berge, J. Rasch, H. Sorantin, C. Keller, B. Tvaroha, D. Ceauşescu, A. Roşcovanu, K. H. Bier, M. Mengel, M. Hřivnáč, M. Matucha, and E. Müller

Subjects
Clinical Biochemistry, General Materials Science, General Medicine, Biochemistry, Analytical Chemistry
Published
1968
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18. Analyse von biologischem Material

Author

O. Fuchs, J. Malur, M. Michlmayr, A. Emr, K. Henning, F. Böhmländer, H. Sorantin, E. Wiegand, H. Bende, D. Rittweger-Heiligmann, G. Peters, K. Baranowsky, A. E. Bühler, B. R. Glutz, J. Rasch, W. Czysz, M. Müller, H. Winkler, A. Niemann, U. Gerhardt, J. Gasparič, I. Pfitzer, P. Gerhards, F. Enzmann, P. Haas, and E. Häbeeli

Subjects
Clinical Biochemistry, General Materials Science, General Medicine, Biochemistry, Analytical Chemistry
Published
1968
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19. Referate

Author

H. Pohl, B. Tvaroha, A. Schmidt, H. Schwarz, M. Bartušek, W. Sternik, H. Wagner, J. Šenkýř, W. Ringsdorf, H. Monien, F. Jančik, M. Meyer zur Heyde, H. Hoffmann, P. -H. Herzhoff, A. Niemann, K. H. Birr, G. Ciuhandu, R. H. Sterzel, J. Hanuš, G. Böck, Z. Stejskal, H. Berge, S. Elias, J. Churáček, K. Cammann, E. Müller, B. R. Glutz, L. Johannsen, J. Rasch, H. Rüssel, J. Kyri, W. Schmidt, B. Roßmann, G. Denk, F. Böhmländer, A. Maurer, K. Baranowsky, D. Rittweger-Heiligmann, C. Kopp, R. Winkler, M. Přibyl, J. Gasparič, W. Dedek, J. Malur, A. Nieman, A. Roşcovanu, I. Bauer, W. Czysz, U. Gerhardt, B. Seifert, P. Gerhards, I. Tryptophan, G. Peters, A. Nimann, E. A. Bühler, and A. Emr

Subjects
Clinical Biochemistry, General Materials Science, General Medicine, Biochemistry, Analytical Chemistry
Published
1971
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20. 1. Elementaranalyse

Author

D. Klockow, H. Bieling, M. Přibyl, K. Henning, J. Malur, A. Sylvester, J. Gusparič, L. Sommer, A. Roscovanu, J. Gasparič, and W. Bögershausen

Subjects
Clinical Biochemistry, General Materials Science, General Medicine, Biochemistry, Analytical Chemistry
Published
1968
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21. 3. Analysenmethoden auf dem Gebiete der Pharmazie

Author

O. Fuchs, J. Malur, A. Roşcovanu, P. Haas, E. Dittrich, K. Baranowsky, G. Peters, U. Haevecker, I. Plank, M. Přibyl, W. Czysz, A. Niemann, Z. Stejskal, and K. Henning

Subjects
Clinical Biochemistry, General Materials Science, General Medicine, Biochemistry, Analytical Chemistry
Published
1968
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23. 1. Analyse von Lebensrnitteln

Author

K. Baranowsky, F. Enzmann, K. Behrends, K. Philippi, J. Gasparič, P. Enzmann, O. Fuchs, A. Niemann, M. Přibyl, B. Rossmann, K. Henning, J. Malinowski, B. E. Glutz, J. Plank, P. Haas, J. Malur, E. Dumkow, K. Wünscher, G. Peters, G. Schiewe, D. Rittweger-Heiligmann, A. Schmidt, H. G. Euleneöfer, K. Bormann, and C. Hesse

Subjects
Engineering, business.industry, Management science, Clinical Biochemistry, Medical laboratory, General Materials Science, Analytical Chemistry (journal), General Medicine, business, Biochemistry, Analytical Chemistry
Published
1968
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24. Analyse von Materialien der Industrie, des Handels und der Landwirtschaft

Author

O. Fuchs, H. Wagner, B. Tvaroha, K. Henning, C. Keller, K. -H. Birr, J. Malinowski, G. Peters, K. H. Neeb, M. Hřivnáč, E. Dittrich, P. Buehler, B. Rossmann, A. Niemann, H. Winkler, E. Müller, K. Baranowsky, L. Johannsen, M. Olivier, H. Bieling, M. Müller, G. Krall, A. Emr, J. Malur, M. Přibyl, P. Haas, E. Dumkow, D. Ceausecscu, J. Gasparič, J. Plank, and W. Czysz

Subjects
Clinical Biochemistry, General Materials Science, General Medicine, Biochemistry, Analytical Chemistry
Published
1968
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25. 4. Analyse von biologischem Material

Author

J. Malur, P. Gerhards, H. Winkler, A. Niemann, A. E. Bühler, M. Olivier, H. Bieling, E. Müller, M. Meyer zur Heyde, D. Rittweger-Heiligmann, M. Mengel, B. Ernst, and K. Henning

Subjects
Clinical Biochemistry, General Materials Science, General Medicine, Analytical Chemistry
Published
1968
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26. 2. Analyse von Materialien der Industrie, des Handels und der landwirtschaft

Author

M. Mengel, W. Schmidt, L. Sommer, M. Přibyl, M. Matucha, K. H. Birr, Martin C. Müller, H. Sorantin, P. Jančik, G. Schiewe, H. Monien, M. Olivier, B. Rossmann, M. Michlmayr, A. Emr, D. CeauŞescu, H. Berge, H. Bieling, J. Malur, H. Pohl, A. Niemann, A. Roscovanu, G. Peters, H. G. Eulenhöfer, J. Plank, W. Sternik, B. R. Glutz, E. Hradský, B. Tvaroha, L. Johannsen, K. Henning, H. M. Lüschow, P. Gerhaeds, W. Bögershausen, G. Knerr, F. Jančik, M. Meyer zur Heyde, and H. A. Rohleder

Subjects
Engineering, business.industry, Clinical Biochemistry, Library science, General Materials Science, Analytical Chemistry (journal), General Medicine, business, Biochemistry, Analytical Chemistry
Published
1968
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27. Qualitative und quantitative Analyse

Author

Martin C. Müller, M. Olivier, M. Přibyl, D. Rittweger-Heiligmann, M. Michlmayr, Lintorf M. Müller, W. Czysz, J. Rasch, J. Plank, C. Hesse, Oliver Fuchs, H. Bieling, J. Malur, J. Gasparič, M. Meyer zur Heyde, H. Hoffmann, B. Tvaroha, A. Schmidt, H. Wagner, A. Niemann, F. Aulinger, Andreas Bühler, K. Henning, G. Ciuhandu, K. Behrends, K. H. Birr, and A. Emr

Subjects
Engineering, business.industry, Management science, Clinical Biochemistry, Medical laboratory, General Materials Science, Analytical Chemistry (journal), General Medicine, business, Biochemistry, Analytical Chemistry
Published
1968
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28. Gewinnung von lumineszierendem Zinksulfid durch Kristallisation aus Salzschmelzen

Author

J. Malur

Subjects
General Materials Science, General Chemistry, Condensed Matter Physics
Abstract

Zinksulfidkristalle wurden aus Bariumthiozinkatschmelzen erhalten. Es werden Einzelheiten uber das Schmelzdiagramm sowie uber die Lumineszenzeigenschaften dotierter Kristalle mitgeteilt. Zinc sulfide crystals were grown from barium thiozincate melts. Details about the melting diagram and the luminescence proporties are given.

Published
1966
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29. 2. Qualitative und quantitative Analyse

Author

O. Fuchs, M. Mengel, K. Henning, M. Meyer zur Heyde, H. Pohl, A. Schmidt, W. Czysz, M. Olivier, J. Malur, B. R. Glutz, L. Johannsen, D. Rittweger-Heiligmann, M. Přibyl, A. Dewald, A. Niemann, H. Bieling, E. Müller, M. Bartušek, J. Plank, J. Gasparič, P. Gerhards, M. Matucha, F. Aulinger, K. H. Birr, H. Monien, A. Emr, B. Tvaroha, B. Rossmann, E. Dewald, P. Haas, E. Dumkow, and C. Hesse

Subjects
Clinical Biochemistry, General Materials Science, General Medicine, Biochemistry, Analytical Chemistry
Published
1968
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30. 2. Analyse von Materialien der Industrie, des Handels und der Landwirtschaft

Author

K. H. Birr, L. Sommer, B. Tvaroha, K. H. Neeb, J. Malinowski, J. Gasparič, P. Haas, G. Winkhaus, M. Müller, A. Niemann, K. Henning, A. E. Bühler, D. Rittweger-Heiligmann, E. Hradský, J. Malur, H. Hoffmann, A. Roşcovanu, H. Bende, B. R. Glutz, K. Behrends, H. -M. Lüschow, W. Sternik, and J. Plank

Subjects
Clinical Biochemistry, General Materials Science, General Medicine, Biochemistry, Analytical Chemistry
Published
1968
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31. Elementaranalyse

Author

D. Klockow, A. Niemann, P. Gerhards, K. Henning, J. Malur, K. Behrends, M. Přibyl, L. Johannsen, and H. Blaha

Subjects
Clinical Biochemistry, General Materials Science, General Medicine, Biochemistry, Analytical Chemistry
Published
1968
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32. Analysis of function-related interactions of ATP, sodium and potassium ions with Na+- and K+-transporting ATPase studied with a thiol reagent as tool

Author

R, Grosse, K, Eckert, J, Malur, and K R, Repke

Subjects
Kidney Medulla, Kinetics, Oxadiazoles, 4-Chloro-7-nitrobenzofurazan, Adenosine Triphosphate, Spectrophotometry, Swine, Sodium, Potassium, Animals, Magnesium, Ribonucleotides, Sodium-Potassium-Exchanging ATPase
Abstract

The paper describes the interaction of ATP, Na+ and K+ with (NaK)-ATPase exploiting the inactivation by reaction with NBD-chloride as an analytical tool for the evaluation of enzyme ligandation with the various effectors. 1. The inactivation of (NaK)-ATPase by reaction with NBD-chloride showing under all conditions studied a pseudo first-order rate rests on the alkylation of thiol groups in or near catalytic centre. ATP bound to catalytic centre prevents from enzyme inactivation by NDD-chloride through protection of these thiol groups from alkylation. Na+ and K+ affect the reactivity of the thiol groups towards NBD-chloride either indirectly via influencing ATP binding or more directly via changing the conformation of catalytic centre. Proceeding from these interrelations, the interaction of the various effectors with the enzyme was analyzed. 2. The K'D-values of various nucleotides determined by our approach correspond to the values obtained by independent methods. As shown for the first time, two catalytic centres per enzyme molecule exist. They exhibit high or low affinity to both ATP and ADP apparently caused by anticooperative interaction of the half-units of the enzyme through intersubunit communication ("half-of-the-sites reactivity"). 3. In the absence of ATP, Na+ or K+ ligandation of (NaK)-ATPase produce opposite effects on the reactivity of the thiol groups of catalytic centres reflecting different changes of their conformation. This corresponds to the well-known antagonistic effect of Na+ and K+ on some partial reactions of (NaK)-ATPase. The Na+ and K+ concentrations required to change thiol reactivity are rather high, i.e. the ionophoric centres for both Na+ and K+ are not readily accessible for cation complexation in the absence of enzyme complexation with ATP. 4. Na+ being without effect on ATP binding to the enzyme also does not influence the inactivating reaction with NBD-chloride while K+ by decreasing ATP binding dramatically decreases the protective effect of ATP. The K+ affinity of the enzyme-ATP complex is by more than two orders of magnitude higher than that of free enzyme. Na+ ligandation of the K+-liganded enzyme-ATP complex reverses the effect of K+ ligandation and produces a protective effect which distinctly surpasses that of the complexation of free enzyme with ATP. Hence, the enzyme molecule carries simultaneously ionophoric centres for both Na+ and K+. 5. The findings that per enzyme molecule ionophoric centres for Na+ and K+, and two catalytic centres with anticooperative interaction coexist corroborate the corresponding basic predictions of the flip-flop concept of (NaK)-ATPase pump mechanism, and explain some peculiar kinetic features of transport and enzyme activities of (NaK)-ATPase.

Published
1978

33. Identification of the ouabain-binding peptide of (nak)-atpase)

Author

M, Kott, E, Spitzer, J, Beer, J, Malur, and K R, Repke

Subjects
Adenosine Triphosphatases, Swine, Animals, Energy Metabolism, Kidney, Ouabain, Peptides, Protein Binding
Abstract

The [3H]-ouabain-(NaK)-ATPase complex when treated in the cold with sodium dodecyl sulfate (SDS) dissociates into a larger and smaller peptide called alpha and beta, resp.. Analysis of the released peptides by SDS-polyacrylamide gel electrophoresis reveals that [3H]-ouabain co-migrates with the alpha-peptide only, being apparently identic with the ouabain receptor molecule. The percentage occupancy of the receptor peptide with [3H]-ouabain can be increased up to 90% evidencing the stabilization of the [3H]-ouabain-alpha-peptide complex by SDS-exposure and release from the oligomeric enzyme. A hypothetic explanation for the seemingly paradoxical stabilising effect of SDS on the complex is offered.

Published
1975

36. [Model studies on participation of a hydrogen bridge binding in the complex formation between cardenolide compounds and Na+ + K+-activated Mg2+-dependent adenosine triphosphate phosphohydrolase]

Author

J, Malur and K R, Repke

Subjects
Adenosine Triphosphatases, Quinine, Biochemical Phenomena, Infrared Rays, Pyridines, Dimethylformamide, Esters, Acetates, Ketones, Biochemistry, Cardiac Glycosides, Butyrates, Lactones, Models, Chemical, Depression, Chemical, Cardanolides, Sulfhydryl Compounds, Protons, Hydrogen
Published
1970

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