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33 results on '"J. J. Carrero"'

1. OP07 Absolute and relative risks of kidney and urological complications in patients with Inflammatory Bowel Disease

Author

Y Yang, J F Ludviggson, O Olén, A Sjölander, and J J Carrero

Subjects
Gastroenterology, General Medicine
Abstract

Background Kidney-related complications are considered relatively common extraintestinal manifestations of inflammatory bowel disease (IBD), but absolute risks are not well characterized. We aimed at providing a comprehensive characterization of the burden of kidney-related complications in people with incident IBD by analysing issued clinical diagnoses and trajectories of estimated glomerular filtration rate (eGFR) in the complete population of Stockholm, Sweden. Methods We included 1,682,795 participants aged 11 or older, free from IBD or chronic kidney disease (CKD) diagnosis and who had a measurement of eGFR during 2006-2018. We analysed the association between developing IBD (time-varying exposure) and the risk of receiving a CKD diagnosis, acute kidney injury (AKI), kidney stones, or secondary amyloidosis, and experiencing the composite of declining their eGFR at least 30% from baseline or kidney failure (collectively termed as CKD progression). Absolute risks were calculated at 5- and 10-year of follow-up, and relative risks were calculated with Cox regression. We compared kidney-related risks overall and for Crohn’s disease (CD) and ulcerative colitis (UC) separately. Results After median 9 years, 10,117 participants newly developed IBD. Their mean age was 45 years. Compared with non-IBD periods, developing IBD associated with higher relative risks: the HR (95% CI) for the risk of receiving a CKD diagnosis was 1.24 (1.10-1.40), 1.11 (1.00-1.24) for the risk of CKD progression, and 1.25 (1.14-1.36) for the composite outcome of these two events. Within 10 years from IBD diagnosis, 6.4% (5.8-7.0%) of participants received a diagnosis of CKD, but 11.4% (10.4-12.4%) had a clinically relevant reduction in eGFR. The risks of AKI (HR 1.97 [1.70-2.29]; 10-y absolute risk 3.6%), kidney stones (HR 1.69 [1.48-1.93]; 10-y risk 5.6%) and secondary amyloidosis (HR 2.77 [1.44-5.35]; 10-y risk 0.2%) were also higher in persons developing IBD compared to non-IBD periods. In general, CD patients exhibited higher absolute and relative kidney risks than UC patients. Conclusion One in ten persons with IBD develop chronic kidney disease within 10 years from diagnosis, with many of these events not being identified through diagnostic codes. This, together with higher risks of AKI and kidney stones emphasizes the need for monitoring of kidney function and established protocols for referral to nephrological/urological care.

Published
2023

2. Association between use of beta-blockers and mortality/morbidity in patients with heart failure with reduced, midrange or preserved ejection fraction and advanced chronic kidney disease

Author

Gianluigi Savarese, Edouard L Fu, Lars H. Lund, Friedo W. Dekker, J. J. Carrero, and A Uijl

Subjects
medicine.medical_specialty, Ejection fraction, business.industry, Atrial fibrillation, medicine.disease, Comorbidity, Pharmacotherapy, Internal medicine, Heart failure, medicine, Cardiology, Cardiology and Cardiovascular Medicine, Heart failure with preserved ejection fraction, business, Beta (finance), Kidney disease
Abstract

Background Beta-blockers reduce mortality and morbidity in patients with heart failure (HF) with reduced ejection fraction (HFrEF). However, patients with advanced chronic kidney disease (CKD) were underrepresented in landmark trials. Purpose We evaluated if beta-blockers are associated with improved survival and cardiovascular outcomes in patients with HFrEF and advanced CKD, and if potential benefits of beta-blockers would extend also to HFpEF and HFmrEF with advanced CKD. Methods We identified 3906 persons with an ejection fraction Results In HFrEF and advanced CKD, 89% received beta-blockers. Overall, median (IQR) age was 81 (74–86) years, 36% were women and median eGFR was 26 (20–28) ml/min/1.73m2. During a median of 1.3 years follow-up, 2086 (53.4%) individuals had a subsequent HF hospitalization, and 2954 (75.6%) individuals died, of which 2089 (70.1%) due to cardiovascular causes. Beta-blocker use was associated with a significant reduction in 5-year all-cause mortality [adjusted hazard ratio (HR) 0.86; 95% confidence interval (CI) 0.76–0.96)] and CV mortality/HF hospitalization (HR 0.87; 95% CI 0.77–0.98). The magnitude of the associations between beta-blocker use and outcomes was similar to that observed for HFrEF patients with mild/moderate CKD [all-cause mortality: 0.85 (95% CI 0.78–0.91); CV mortality/HF hospitalization: 0.88 (95% CI 0.82–0.96)]. Adjusted HRs were 0.88 (95% CI 0.77–1.02) and 1.07 (95% CI 0.92–1.24) for individuals with HFpEF and advanced CKD and 0.95 (95% CI 0.80–1.13) and 1.13 (95% CI 0.94–1.36) for individuals with HFmrEF and advanced CKD, for all-cause mortality and CV mortality/HF hospitalization, respectively. Conclusion Despite lack of trial evidence, the use of beta-blockers in patients with HFrEF and advanced CKD was high in routine Swedish care, and was independently associated with reduced mortality to the same degree as HFrEF with moderate CKD. However, these benefits were not observed in patients with HFpEF or HFmrEF with severe CKD. Funding Acknowledgement Type of funding source: None

Published
2020

3. Multiplex proteomics for prediction of major cardiovascular events in type 2 diabetes

Author

Nowak, C., Carlsson, A. C., Östgren, C. J., Nyström, F. H., Alam, M., Feldreich, T. R., Sundström, Johan, Roig, J. J. Carrero, Leppert, Jerzy, Hedberg, Pär O., Cordeiro, A. C., Lind, Lars, Ingelsson, E., Fall, Tove, Arnlöv, J., Nowak, C., Carlsson, A. C., Östgren, C. J., Nyström, F. H., Alam, M., Feldreich, T. R., Sundström, Johan, Roig, J. J. Carrero, Leppert, Jerzy, Hedberg, Pär O., Cordeiro, A. C., Lind, Lars, Ingelsson, E., Fall, Tove, and Arnlöv, J.

Published
2018

4. CKD NUTRITION, INFLAMMATION AND OXIDATIVE STRESS

Author

Abdelouahed Khalil, Anteo Di Napoli, Stephen Zewinger, Mariusz Flisiński, Domenico Trimboli, Maurizio Bossola, Marina Biagioli, Odile Azoulay, Michele Buemi, Piergiorgio Bolasco, Andreea Ilyes, Ángeles Álvarez, Valeria Cernaro, Polichronis Alivanis, Gabriel Stefan, Mehmet Haberal, Marcelo Costa Batista, Jerzy Chudek, Mujdat Batur Canoz, Branka Mitic, Abdul Rashid Qureshi, Anna Stefańska, Christiaan L. Meuwese, Anna Kamińska, Vera Jankowski, Yeon Soon Jung, Luisa Sereni, Rafał Donderski, Ho Sik Shin, Ciprian Stoica, Peter Stenvinkel, Insa E. Emrich, Rosanna Coppo, Boris Zingerman, Rachid Saile, Sonja Radenkovic, Yener Koc, Ilona Miśkowiec-Wiśniewska, Tatjana Cvetkovic, Danilo Fliser, Grigore Dogaru, Simonetta Palleschi, Milica Bozic, Urszula Spiechowicz-Zatoń, Kevin J. Woollard, Serpil Nebioğlu, Erik W. Holy, Hark Rim, Mahmut Gok, Adriana Arena, Iryna Dudar, Goran Paunovic, Elvira Fernández, Maria Inês Barreto-Silva, Jacek Manitius, Naohito Isoyama, Demet Yavuz, Michael Böhm, Jose M. Valdivielso, Paolo Maria Ghezzi, Silvia Regina Manfredi, Paweł Stróżecki, Yusuf Oguz, Maria L Lusini, Miomir Stojanovic, Gabriel Mircescu, Mikio Sugano, Paula Maria Gliga, Zeynep Bal, Mirela Liana Gliga, Elisa Loiacono, Hilmi Umut Ünal, Viktoria Alekseeva, Sun Chul Kim, Hyun Yul Rhew, José Tarcísio Giffoni de Carvalho, Foteini Lamprianou, Ekrem Kara, Sang-Kyung Jo, Barbara E. Stähli, Abdulkadir Unsal, Francesco Franco, Murat Karaman, Shunsuke Goto, Sumie Goto, Richard J. Johnson, Tamer Sakaci, Christophe Dubois, Roman Junik, Nikolaos Karvouniaris, Nikitas Moschos, Timo Speer, Kathleen Claes, Maria Teresa Ingegneri, Gunnar H. Heine, Pieter Evenepoel, Zahava Gamzo, Juan Jesus Carrero, Keiji Kono, Bertrand Gondouin, Sylwia Rotkegel, Mehmet Kanbay, Sebahattin Sari, Roxanne Darbousset, Shinichi Nishi, Andrzej Brymora, Bahar Gurlek Demirci, Vincenzo Bellizzi, Joanna Siódmiak, Domenico Di Lallo, Ye Na Kim, Latife Atasoy Karakas, Adam M. Zawada, Alexander Akhmedov, Mustafa Sevinc, Tuncay Sahutoglu, Hrvoje Cvija, Giovanni G. Camici, Ayse C Akbasli, Mahmut I Yilmaz, Christos Paliouras, Karolina Paunovic, Bulent Erbay, Carla Cavalheiro da Silva Lemos, Frances Costa-Silva, Marina Davoli, Liesbeth Viaene, Fatma Nurhan Ozdemir, Mehtap Erkmen Uyar, Kentaro Nakai, Adrian Covic, Danijela Tasic, Jarosław Ciepał, Mauro Atti, Gunnar Heine, Stéphane Burtey, Domenico Santoro, Benaya Rozen-Zvi, Simone Vargas, Lucia Rohrer, Gaetano Montalto, Hakki Cetinkaya, Turan Colak, Bert Bammens, Alessandro Amore, Tayfun Eyileten, Paul Leurs, Liliana Viasu, Theodoros Haviatsos, Yalcin Solak, Antonino Sidoti, Carmen de Pablo, Tayfun EyIeten, Vincenzo Savica, Maria Dolores Sanchez-Niño, Yasemin Gulcan Kurt, Gordana Kocic, Joachim Jankowski, Andrea Pisacane, Katarzyna Wyskida, Kenan Keven, Sunna Snaedal, Zorica Dimitrijevic, Nikolaos Papagiannis, Abdülgaffar Vural, Taner Basturk, Mutlu Saglam, Björn Anderstam, Hilmi Umut Unal, Serena Chicca, Aline Trevisan Peres, Maria Eugênia Fernandes Canziani, Alberto Ortiz, Mahmut Ilker Yilmaz, Bengt Lindholm, Stéphane Poitevin, Kiryong Park, Paolo M. Ghezzi, Antonio Pisani, Marialuisa Caiazzo, Noreddine Ghalim, Viktor Krot, Silvia Lucisano, Maria Aparecida Dalboni, Eleonora Riccio, Sarah Seiler, Giorgos Ntetskas, Oskar Svedberg, Carmela Aloisi, Sangeon Gwoo, Peter Bárány, Maria Bożentowicz-Wikarek, Alpaslan Altunoglu, O.M. Loboda, Elbis Ahbap, Dariusz Klein, Tuncer Cayci, Siren Sezer, Rosaria Lupica, Giuseppe Vita, Ruben Poesen, Björn Meijers, Nagba Yendoubé Gbandjaba, Magdalena Olszanecka-Glinianowicz, Burak Sayin, Won Yong Cho, Beata Marie Redublo Quinto, J. J. Carrero, Yuriko Yonekura, Felix C. Tanner, Miguel Cendoroglo, Olof Heimbürger, Cuneyt Akgol, Paola Michelozzi, Sarah Triem, Yoshiharu Ito, Emanouil Anastasakis, GrażYna Odrowa˛ż-Sypniewska, Caren Cristina Grabulosa, Aniceta Brzozowska, Hyung Kyu Kim, Marek Kretowicz, Maria Inês Barreto Silva, Thereza Christina Barja-Fidalgo, Yusuke Yamashita, Hideki Fujii, Simona Stancu, Luigi Tazza, Giuseppe Palladino, Nilufer Bayraktar, Rachel Bregman, Rahman Yavuz, Cengizhan Acikel, Barbara Rossi, Cristina Capusa, Massimo Sabbatini, Myung Gyu Kim, Guido Bellinghieri, Renata de Souza Mendes, Dorin Tarta, Radmila Veličković-Radovanović, Kyrill S. Rogacev, Kostantinos Roufas, and Thomas F. Lüscher

Subjects
Transplantation, medicine.medical_specialty, Framingham Risk Score, Cardiovascular History, business.industry, Hazard ratio, Renal function, medicine.disease, Gastroenterology, Endocrinology, Nephrology, Diabetes mellitus, Internal medicine, medicine, Myocardial infarction, Prospective cohort study, business, Kidney disease
Abstract

Introduction and Aims: Serum p-cresyl sulfate associates with cardiovascular disease in patients at different stages of chronic kidney disease. p-Cresyl sulfate concentrations are determined by intestinal uptake of p-cresol, human metabolism to p-cresyl sulfate and renal clearance. Whether intestinal uptake of p-cresol itself is associated with cardiovascular disease in patients with renal disease has not been studied to date. Methods: We performed a prospective study in patients with chronic kidney disease stage 1-5 (clinicaltrials.gov NCT00441623). Intestinal uptake of p-cresol, under steady state conditions, was estimated from 24h urinary excretion of p-cresyl sulfate. Primary endpoint was time to first cardiovascular event, i.e. cardiac death, myocardial infarction/ischemia, ventricular arythmia, cardiovascular surgery, cerebrovascular accident or symptomatic peripheral arterial disease. Statistical analysis was done using Kaplan Meier estimates and Cox proportional hazard analyses. Results: In a cohort of 200 patients, median 24h urinary excretion of p-cresyl sulfate was 457.47 µmol (IQR 252.68-697.17). After a median follow-up of 52 months, 25 patients reached the primary endpoint (tertile 1/2/3: 5/6/14 events, log rank P 0.0368, see figure). Higher urinary excretion of p-cresyl sulfate was related with cardiovascular events (univariate hazard ratio per 100 µmol increase: 1.112, P 0.0015). In multivariate analysis, urinary excretion of p-cresyl sulfate remained a predictor of cardiovascular events, independent of markers of renal function (Hazard ratio 1.120, P 0.0022) and in different models with other cardiovascular risk factors (Framingham risk factors, cardiovascular history, diabetes mellitus and biochemical parameters). The independent association between urinary excretion of p-cresyl sulfate and outcome persisted after correction for serum p-cresyl sulfate. Conclusions: Intestinal uptake of p-cresol associates with cardiovascular disease independent of renal function. Insights into mechanisms governing intestinal generation and absorption of p-cresol may lead to identification of novel therapeutic targets to reduce cardiovascular disease risk in patients with chronic kidney disease.

Published
2014

5. SP660HIGH-VOLUME ON-LINE HEMODIAFILTRATION MAY PREVENT PROTEIN-ENERGY WASTING IN HEMODIALYSIS PATIENTS: A 1-YEAR PROSPECTIVE CONTROLLED STUDY

Author

Antonio Mora, Cristina Castro-Alonso, José Luis Górriz, Mercedes González-Moya, Luis M Pallardó, Ana Ávila, Mariola D Molina, Sandra Beltrán, Denis Fouque, Pablo Molina, Julia Kanter, J. J. Carrero, Nuria Estañ, and Belén Vizcaíno

Subjects
Transplantation, medicine.medical_specialty, business.industry, medicine.medical_treatment, Urology, Protein energy wasting, medicine.disease, Cachexia, Volume (thermodynamics), Nephrology, On line hemodiafiltration, medicine, Hemodialysis, business
Published
2018

6. Epidemiology and outcome research in CKD 5D

Author

L. Coentrao, C. Ribeiro, C. Santos-Araujo, R. Neto, M. Pestana, W. Kleophas, A. Karaboyas, Y. LI, J. Bommer, R. Pisoni, B. Robinson, F. Port, G. Celik, B. Burcak Annagur, M. Yilmaz, T. Demir, F. Kara, K. Trigka, P. Dousdampanis, N. Vaitsis, S. Aggelakou-Vaitsi, K. Turkmen, I. Guney, F. Turgut, L. Altintepe, H. Z. Tonbul, E. Abdel-Rahman, P. Sclauzero, G. Galli, G. Barbati, M. Carraro, G. O. Panzetta, M. Van Diepen, M. Schroijen, O. Dekkers, F. Dekker, A. Sikole, G. Severova- Andreevska, L. Trajceska, S. Gelev, V. Amitov, S. Pavleska- Kuzmanovska, H. Rayner, R. Vanholder, M. Hecking, B. Jung, M. Leung, F. Huynh, T. Chung, S. Marchuk, M. Kiaii, L. Er, R. Werb, C. Chan-Yan, M. Beaulieu, P. Malindretos, P. Makri, G. Zagkotsis, G. Koutroumbas, G. Loukas, E. Nikolaou, M. Pavlou, E. Gourgoulianni, M. Paparizou, M. Markou, E. Syrgani, C. Syrganis, J. Raimann, L. A. Usvyat, V. Bhalani, N. W. Levin, P. Kotanko, X. Huang, P. Stenvinkel, A. R. Qureshi, U. Riserus, T. Cederholm, P. Barany, O. Heimburger, B. Lindholm, J. J. Carrero, J. H. Chang, J. Y. Sung, J. Y. Jung, H. H. Lee, W. Chung, S. Kim, J. S. Han, K. Y. Na, A. Fragoso, A. Pinho, A. Malho, A. P. Silva, E. Morgado, P. Leao Neves, N. Joki, Y. Tanaka, M. Iwasaki, S. Kubo, T. Hayashi, Y. Takahashi, K. Hirahata, Y. Imamura, H. Hase, C. Castledine, J. Gilg, C. Rogers, Y. Ben-Shlomo, F. Caskey, J. S. Sandhu, G. S. Bajwa, S. Kansal, J. Sandhu, A. Jayanti, M. Nikam, L. Ebah, A. Summers, S. Mitra, J. Agar, A. Perkins, R. Simmonds, A. Tjipto, S. Amet, V. Launay-Vacher, M. Laville, A. Tricotel, C. Frances, B. Stengel, J.-Y. Gauvrit, N. Grenier, G. Reinhardt, O. Clement, N. Janus, L. Rouillon, G. Choukroun, G. Deray, A. Bernasconi, R. Waisman, A. P. Montoya, A. A. Liste, R. Hermes, G. Muguerza, R. Heguilen, E. L. Iliescu, V. Martina, M. A. Rizzo, P. Magenta, L. Lubatti, G. Rombola, M. Gallieni, C. Loirat, H. Mellerio, M. Labeguerie, B. Andriss, E. Savoye, M. Lassale, C. Jacquelinet, C. Alberti, Y. Aggarwal, J. Baharani, S. Tabrizian, S. Ossareh, M. Zebarjadi, P. Azevedo, F. Travassos, I. Frade, M. Almeida, J. Queiros, F. Silva, A. Cabrita, R. Rodrigues, C. Couchoud, J. Kitty, S. Benedicte, C. Fergus, C. Cecile, B. Sahar, V. Emmanuel, J. Christian, E. Rene, H. Barahimi, M. Mahdavi-Mazdeh, M. Nafar, M. Petruzzi, M. De Benedittis, M. Sciancalepore, L. Gargano, P. Natale, M. C. Vecchio, V. Saglimbene, F. Pellegrini, G. Gentile, P. Stroumza, L. Frantzen, M. Leal, M. Torok, A. Bednarek, J. Dulawa, E. Celia, R. Gelfman, J. Hegbrant, C. Wollheim, S. Palmer, D. W. Johnson, P. J. Ford, J. C. Craig, G. F. Strippoli, M. Ruospo, B. El Hayek, B. Hayek, E. Baamonde, E. Bosch, J. I. Ramirez, G. Perez, A. Ramirez, A. Toledo, M. M. Lago, C. Garcia-Canton, M. D. Checa, B. Canaud, B. Lantz, A. Granger-Vallee, P. Lertdumrongluk, N. Molinari, J. Ethier, M. Jadoul, B. Gillespie, C. Bond, S. Wang, T. Alfieri, P. Braunhofer, B. Newsome, M. Wang, B. Bieber, M. Guidinger, L. Zuo, X. Yu, X. Yang, J. Qian, N. Chen, J. Albert, Y. Yan, S. Ramirez, M. Beresan, A. Lapidus, M. Canteli, A. Tong, B. Manns, J. Craig, G. Strippoli, M. Mortazavi, B. Vahdatpour, S. Shahidi, A. Ghasempour, D. Taheri, S. Dolatkhah, A. Emami Naieni, M. Ghassami, M. Khan, K. Abdulnabi, P. Pai, M. Vecchio, M. A. Muqueet, M. J. Hasan, M. A. Kashem, P. K. Dutta, F. X. Liu, L. Noe, T. Quock, N. Neil, G. Inglese, M. Motamed Najjar, B. Bahmani, A. Shafiabadi, J. Helve, M. Haapio, P.-H. Groop, C. Gronhagen-Riska, P. Finne, R. Sund, M. Cai, S. Baweja, A. Clements, A. Kent, R. Reilly, N. Taylor, S. Holt, L. Mcmahon, M. Carter, F. M. Van der Sande, J. Kooman, R. Malhotra, G. Ouellet, E. L. Penne, S. Thijssen, M. Etter, A. Tashman, A. Guinsburg, A. Grassmann, C. Barth, C. Marelli, D. Marcelli, G. Von Gersdorff, I. Bayh, L. Scatizzi, M. Lam, M. Schaller, T. Toffelmire, Y. Wang, P. Sheppard, L. Neri, V. A. Andreucci, L. A. Rocca-Rey, S. V. Bertoli, D. Brancaccio, G. De Berardis, G. Lucisano, D. Johnson, A. Nicolucci, C. Bonifati, S. D. Navaneethan, V. Montinaro, M. Zsom, A. Bednarek-Skublewska, G. Graziano, J. N. Ferrari, A. Santoro, A. Zucchelli, G. Triolo, S. Maffei, S. De Cosmo, V. M. Manfreda, L. Juillard, A. Rousset, F. Butel, S. Girardot-Seguin, T. Hannedouche, M. Isnard, Y. Berland, P. Vanhille, J.-P. Ortiz, G. Janin, P. Nicoud, M. Touam, E. Bruce, B. Grace, P. Clayton, A. Cass, S. Mcdonald, Y. Furumatsu, T. Kitamura, N. Fujii, S. Ogata, H. Nakamoto, K. Iseki, Y. Tsubakihara, C.-C. Chien, J.-J. Wang, J.-C. Hwang, H.-Y. Wang, W.-C. Kan, N. Kuster, L. Patrier, A.-S. Bargnoux, M. Morena, A.-M. Dupuy, S. Badiou, J.-P. Cristol, J.-M. Desmet, V. Fernandes, F. Collart, N. Spinogatti, J.-M. Pochet, M. Dratwa, E. Goffin, J. Nortier, D. S. Zilisteanu, M. Voiculescu, E. Rusu, C. Achim, R. Bobeica, S. Balanica, T. Atasie, S. Florence, S. Anne-Marie, L. Michel, C. Cyrille, A. Strakosha, N. Pasko, S. Kodra, N. Thereska, A. Lowney, E. Lowney, R. Grant, M. Murphy, L. Casserly, T. O' Brien, W. D. Plant, J. Radic, D. Ljutic, V. Kovacic, M. Radic, K. Dodig-Curkovic, M. Sain, I. Jelicic, T. Hamano, C. Nakano, S. Yonemoto, A. Okuno, M. Katayama, Y. Isaka, M. Nordio, A. Limido, M. Postorino, M. Nichelatti, M. Khil, I. Dudar, V. Khil, I. Shifris, M. Momtaz, A. R. Soliman, M. I. El Lawindi, P. Dzekova-Vidimliski, S. Pavleska-Kuzmanovska, I. Nikolov, G. Selim, T. Shoji, R. Kakiya, N. Tatsumi-Shimomura, Y. Tsujimoto, T. Tabata, H. Shima, K. Mori, S. Fukumoto, H. Tahara, H. Koyama, M. Emoto, E. Ishimura, Y. Nishizawa, and M. Inaba

Subjects
Transplantation, medicine.medical_specialty, Nephrology, business.industry, Epidemiology, Medicine, business, Intensive care medicine, Outcome (game theory)
Published
2012

7. Peritoneal dialysis

Author

J. Liu, Y. Liu, Y. Xu, X. Zhao, J. Qian, B. Sun, C. Xing, R. Kanda, C. Hamada, T. Nakano, K. Wakabayashi, H. Io, S. Horikoshi, Y. Tomino, N. Ishimatsu, T. Miyamoto, H. Morimoto, J. Nakamata, R. Baba, K. Kanegae, R. Serino, N. Kabashima, Y. Otsuji, Y. Doi, M. Tamura, T. Kusumoto, K. Fukami, S.-I. Yamagishi, S. Ueda, Y. Kaida, T. Hazama, Y. Nakayama, R. Ando, N. Obara, S. Okuda, M. Matsumoto, Y. Furuno, H. Bang-Gee, L. Mazzotta, A. Rosati, A. Carlini, V. T. Henriques, E. Zangiacomi Martinez, J. C. Divino-Filho, R. Pecoits-Filho, J. A. Cardeal Da Costa, T. Gama Axelsson, B. Lindholm, J. J. Carrero, O. Heimburger, P. Stenvinkel, A. R. Qureshi, M. Akazawa, T. Uno, E. Kanda, Y. Maeda, M. Aktsiali, S. Antonopoulou, K. Tsiolaki, N. Bakirtzi, A. Patrinou, M. Georgopoulou, P. Liaveri, N. Afentakis, G. Tsirpanlis, T. Hasegawa, H. Nishiwaki, M. Hirose, D. Komukai, H. Tayama, F. Koiwa, A. Yoshimura, S. L. Lui, S. Lui, S. Yung, C. Tang, F. Ng, W. K. Lo, T. M. Chan, H. M. Koo, F. M. Doh, D. E. Yoo, H. J. Oh, T.-H. Yoo, K. H. Choi, S.-W. Kang, D. S. Han, S. H. Han, N. Fernandes, M. G. Bastos, M. R. Gianotti Franco, A. Chaoubah, M. D. Gloria Lima, S. Kang, J. Do, K. Cho, J. Park, K. Yoon, J.-B. Chen, B.-C. Cheng, T.-C. Chen, Y.-J. Su, C.-H. Wu, Y. Park, J. Jeon, M. Tsikeloudi, P. Pateinakis, K. Patsatsi, E. Manou, D. Sotiriadis, D. Tsakiris, L. Teixeira, A. Rodrigues, M. J. Carvalho, A. Cabrita, D. Mendonca, M. Bruschi, G. Candiano, L. Santucci, S. Luzio, R. Cannavo, G. M. Ghiggeri, E. Verrina, Y. Varadarajan, B. Raju, K.-H. Cho, J.-W. Park, K.-W. Yoon, T.-W. Kim, M. Kimmel, N. Braun, J. Latus, M. D. Alscher, D. Struijk, S. Van Esch, R. T. Krediet, T. Van den Beukel, T. Hoekstra, L. Tirapani, K. De Andrade Bastos, M. Bastos, F. Dekker, T. Yasuhisa, H. Kanai, K. Harada, Y. Kawai, H. Sugiyama, Y. Ito, K. Tsuruya, H. Yoshida, H. Maruyama, S. Goto, M. Nakayama, H. Nakamoto, H. Morinaga, S. Matsuo, H. Makino, M. C. DI Gioia, P. Gallar, N. Laso, I. Rodriguez, G. Cobo, A. Oliet, J. Hynostroza, J. C. Herrero, C. Mon, M. Ortiz, A. Vigil, T. Tomo, J. Portoles, S. Uta, A. M. Tato, P. Lopez-Sanchez, M. Rivera, R. Rodriguez-Pena, G. Del Peso, M. Ortega, C. Felipe, E. Tsampikaki, G. Aperis, A. Kaikis, C. Paliouras, N. Karvouniaris, M. Maragaki, P. Alivanis, B. Kortus-Gotze, T. Hoferhusch, J. Hoyer, F. Martino, M. Kaushik, M. P. Rodighiero, C. Creapldi, C. Ronco, A. Lacquaniti, V. Donato, M. R. Fazio, S. Lucisano, V. Cernaro, R. Lupica, M. Buemi, C. Aloisi, N. Bavbek Ruzgaresen, S. Secilmis, H. Yilmaz, A. Akcay, M. Duranay, N. Akalin, M. R. Altiparmak, S. Trabulus, A. S. Yalin, R. Ataman, K. Serdengecti, K. Schneider, B. Bator, B. Niko, F. Peter, C. Ulmer, L. Joerg, K. Martin, B. Dagmar, O. German, R. Fabian, D. Juergen, S. Stephan, A. Dominik, P. Fritz, B. Rettenmaier, S. Hirschburger, S. Segerer, D. Biegger, T. Lang, G. Ott, M. Habib, M. Korte, M. Hagen, F. Dor, M. Betjes, R. Zietse, C. Scharpf, T. I. Chang, D. H. Shin, D.-S. Han, H. Y. Choi, Y. K. Lee, B. S. Kim, T. H. Yoo, H. C. Park, H. Y. Lee, N. Horimoto, K. Tuji, S. Kitamura, R. Isshiki, M. Iwagami, D. Tsutsumi, Y. Mochida, K. Ishioka, M. Oka, K. Maesato, H. Moriya, T. Ohtake, S. Hidaka, S. Kobayashi, C. Higuchi, Y. Tanihata, M. Ishii, H. Sugimoto, N. Sato, A. Kyono, T. Ogawa, H. Nishimura, K. Otsuka, J.-Y. Do, C. Du Halgouet, A. Latifa, V. Anne Sophie, D. Emmanuel, R. Christine, V. Francois, T. Grzelak, L. Czyzewska-Majchrzak, M. Kramkowska, H. Witmanowski, K. Czyzewska, K. Janda, M. Krzanowski, P. Dumnicka, W. Sulowicz, M. Rroji, S. Seferi, M. Barbullushi, E. Likaj, E. Petrela, N. Thereska, G. Cabiddu, E. Dessi, A. Arceri, P. Laura, E. Manca, M. Conti, R. Cao, A. Pani, C.-T. Liao, O. Vega Vega, A. Mendoza de la Garza, R. Correa-Rotter, A. Ueda, K. Nagai, M. Morimoto, A. Hirayama, S. Owada, Y. Tonozuka, C. Saito, K. Yamagata, A. Matsuda, Y. Tayama, M. Iwanaga, C. Noiri, M. Hatano, T. Kiba, K. Kanozawa, H. Katou, H. Hasegawa, T. Mitarai, S. Ros-Ruiz, L. Fuentes-Sanchez, C. Jironda-Gallegos, E. Gutierrez-Vilches, P. Garcia-Frias, D. Hernandez-Marrero, S. Lee, X. Lai, W. Chen, Z. Guo, M. Braide, V. Cristina, S. G. Popa, M. Maria, M. Eugen, P. DI Loreto, N. Spahia, L. O. Sanchez Macias, K. I. Lares Castellanos, J. A. Hernandez Pacheco, R. Correa Rotter, A. Pedro Ventura, S. Olivia, V. Joana, F. Francisco, C. Maria Joao, C. Antonio, A. S. Rodrigues, N. Atas, Y. Erten, K. Onec, S. Inal, S. Topal, A. Akyel, B. Celik, G. U. Okyay, Y. Tavil, M. Zeiler, T. Monteburini, R. M. Agostinelli, R. Marinelli, S. Santarelli, C. Yaylaci, G. Sahin, G. Guz, S. Sindel, A. Pinho, A. Malho Guedes, A. Fragoso, H. Carreira, I. Pinto, I. Bernardo, P. Leao, B. Kusnierz-Cabala, A. Krasniak, E. Chowaniec, B. Tabor-Ciepiela, K. Turkmen, O. Ozbek, M. Kayrak, C. Samur, I. Guler, H. Z. Tonbul, K. Rusai, R. Herzog, K. Kratochwill, L. Kuster, C. Aufricht, C.-M. Meier, D. Fliser, M. K. Schilling, M. Klingele, M. Fukasawa, M. Takeda, M. Kamiyama, Y. R. Song, H. J. Kim, S. G. Kim, J.-K. Kim, J. W. Noh, J. W. Yoon, and J.-R. Koo

Subjects
Transplantation, Nephrology
Published
2012

9. Relationship between renal function and renal volume in autosomal dominant polycystic kidney disease: cross-sectional study

Author

M J, Torres-Sánchez, E, Ávila-Barranco, R J, Esteban de la Rosa, R, Fernández-Castillo, M A, Esteban, J J, Carrero, M, García-Valverde, and J A, Bravo-Soto

Abstract

To determine in patients with autosomal dominant polycystic kidney disease the relationship between total renal volume (the sum of both kidneys, TRV) as measured by magnetic resonance and renal function; and its behaviour according to sex and the presence of arterial hypertension, hypercholesterolaemia and hyperglycemia.Cross-sectional study including patients with autosomal dominant polycystic kidney disease who underwent periodic reviews at Nephrology external consultations at Hospital de las Nieves de Granada, and who underwent an magnetic resonance to estimate renal volume between January 2008 and March 2011.We evaluated 67 patients (59.7% women, average age of 48±14.4 years) and found a significant positive association between TRV and serum creatinine or urea, which was reversed compared with estimated glomerular filtration by MDRD-4 and Cockcroft-Gault. Women showed an average serum creatinine level and a significantly lower TRV level compared with males. Subgroups affected by arterial hypertension and hyperuricemia presented average values for serum creatinine and urea, higher for TRV and lower for estimated glomerular filtration. The hypercholesterolaemia subgroup showed higher average values for urea and lower for estimated glomerular filtration, without detecting significant differences compared with TRV.The volume of polycystic kidneys measured by magnetic resonance is associated with renal function, and can be useful as a complementary study to monitor disease progression. The presence of arterial hypertension, hyperuricemia or hypercholesterolaemia is associated with a poorer renal function.

Published
2015

11. SP006VASOPRESSIN-RELATED COPEPTIN IS A NOVEL PREDICTOR OF EARLY ENDOTHELIAL DYSFUNCTION IN PATIENTS WITH ADULT POLYCYSTIC KIDNEY DISEASE

Author

Jonas Axelsson, Oktay Oymak, Ozcan Orscelik, Ozkan Gungor, Murat Hayri Sipahioglu, Ismail Kocyigit, Mahmut Ilker Yilmaz, Bulent Tokgoz, J. J. Carrero, Ahmet Sen, Aydin Unal, and Eray Eroglu

Subjects
Transplantation, medicine.medical_specialty, Copeptin, Adult Polycystic Kidney Disease, Nephrology, business.industry, Internal medicine, Cardiology, Medicine, In patient, Endothelial dysfunction, business, medicine.disease
Published
2017

13. MP330LOWER CALCIUM IS ASSOCIATED WITH KIDNEY FUNCTION DECLINE IN INDIVIDUALS WITH CHRONICKIDNEY DISEASE

Author

Abdul Rashid Qureshi, Joris I. Rotmans, Marie Evans, Marc G. Vervloet, M. van Diepen, Cynthia J. Janmaat, Peter Bárány, Alessandro Gasparini, J. J. Carrero, Carl Gustaf Elinder, Johan Ärnlöv, and Friedo W. Dekker

Subjects
Transplantation, chemistry, Nephrology, business.industry, Physiology, Medicine, Renal function, chemistry.chemical_element, Disease, Calcium, business
Published
2016

14. CKD NUTRITION

Author

A. Yoshifuji, S. Wakino, J. Irie, H. Minakuchi, K. Hasegawa, H. Tokuyama, K. Hayashi, H. Itoh, H. Xu, X. Huang, U. Riserus, T. Cederholm, J. Arnlov, P. Sjogren, B. Lindholm, J. J. Carrero, R. Poesen, K. Windey, P. Evenepoel, V. De Preter, K. Verbeke, B. Meijers, C. L. De Roij Van Zuijdewijn, M. J. Nube, M. L. Bots, P. J. Blankestijn, M. A. Van Den Dorpel, P. M. Ter Wee, M. P. Grooteman, R. Masereeuw, and P. H. Van Den Broek

Subjects
Transplantation, Nephrology
Published
2014

15. Protein-Energy Malnutrition/Wasting During Peritoneal Dialysis

Author

Jonas Axelsson, M. Chan, Olof Heimbürger, J. J. Carrero, Peter Stenvinkel, and Bengt Lindholm

Subjects
medicine.medical_specialty, Protein–energy malnutrition, business.industry, Esrd patient, medicine.medical_treatment, Internal medicine, Medicine, medicine.symptom, business, medicine.disease, Gastroenterology, Wasting, Peritoneal dialysis
Published
2009

16. Low serum fetuin-A concentration predicts poor outcome only in the presence of inflammation in prevalent haemodialysis patients

Author

G, Metry, P, Stenvinkel, A R, Qureshi, J J, Carrero, M I, Yilmaz, P, Bárány, S, Snaedal, O, Heimbürger, B, Lindholm, and M E, Suliman

Subjects
Inflammation, Male, Cross-Sectional Studies, Renal Dialysis, alpha-2-HS-Glycoprotein, Humans, Kidney Failure, Chronic, Female, Blood Proteins, Middle Aged, Biomarkers, Aged
Abstract

Fetuin-A, a negative acute phase protein that inhibits vascular calcification, has a controversial association with mortality in chronic kidney disease (CKD) patients. Chronic inflammation, which is common in CKD, may promote vascular calcification.We investigated the impact of inflammation on the relationship between serum fetuin-A and mortality (42 months) in 222 prevalent haemodialysis (HD) patients.Serum fetuin correlated negatively with comorbidity score (assessed by Davies score) and circulating inflammatory markers. Patients with low fetuin-A levels (median) had higher mortality (Hazard ratio 'HR' 2.2; CI 1.4-3.5, P0.001), but this association was lost after adjustment for age, gender, comorbidities score, dialysis vintage and inflammation (CRPmedian). In inflamed patients with low fetuin a significantly independent association with mortality (HR 2.3; CI 1.2-4.5, P = 0.01) was observed compared to non-inflamed patients with high fetuin-A, after adjusting for the same variables. Non-inflamed patients with low fetuin-A and inflamed patients with high fetuin-A did not have increased mortality compared to non-inflamed patients with high fetuin-A.The results show that low levels of serum fetuin-A are associated with increased mortality in HD patients only in the presence of inflammation. This suggests that coexistence of a low serum fetuin-A level and low-grade inflammation exerts an additive effect on the risk of death in HD patients.

Published
2008

17. Clinical and biochemical implications of low thyroid hormone levels (total and free forms) in euthyroid patients with chronic kidney disease

Author

J J, Carrero, A R, Qureshi, J, Axelsson, M I, Yilmaz, S, Rehnmark, M R, Witt, P, Bárány, O, Heimbürger, M E, Suliman, A, Alvestrand, B, Lindholm, and P, Stenvinkel

Subjects
Adult, Male, Thyroid Hormones, Interleukin-6, Wasting Syndrome, Middle Aged, Prognosis, C-Reactive Protein, Cardiovascular Diseases, Renal Dialysis, Humans, Kidney Failure, Chronic, Triiodothyronine, Female, Epidemiologic Methods, Biomarkers, Aged
Abstract

In this study, we explore the associations of decreased thyroid hormone levels with inflammation, wasting and survival in biochemically euthyroid patients with end-stage renal disease (ESRD).After exclusion of 23 patients with thyroid-stimulating hormone (TSH) values outside the normal range (0.1-4.5 mIU L(-1)), 187 clinically and biochemically euthyroid incident ESRD stage 5 patients starting dialysis were followed for a median of 20 (range 1-60) months. Measurements of total and free forms of thyroid hormones, s-albumin, hs-CRP, interleukin (IL)-6, vascular adhesion molecule (VCAM)-1 and insulin-like growth factor 1 (IGF-1) were performed at baseline.In this population, 17 out of 210 patients (8%) were defined as subclinically hypothyroid. Multivariate analysis, according to receiver operating characteristic (ROC) curves, showed that mortality was best predicted by total triiodothyronine (T3). When using the cut-off levels derived from ROC, low T3 levels were associated with increased inflammation (higher hs-CRP, IL-6 and VCAM-1) and lower concentration of both s-albumin and IGF-1. Finally, low T3 but not low free triiodothyronine was associated with worse all-cause (Likelihood ratio = 45.4; P0.0001) and cardiovascular mortality (Likelihood ratio = 47.8; P0.0001) after adjustment for confounding factors.This study showed that low T3 levels are independent predictors of all-cause and also cardiovascular disease mortality in biochemically euthyroid patients, perhaps due to an intimate association with inflammation. Based on these results, the use of T3 levels in studies assessing the relationship between thyroid dysfunction and mortality risk is recommended.

Published
2007

18. Peritoneal dialysis - clinical

Author

Ozlem Yayar, J. J. Carrero, Simon J. Davies, Olof Heimbürger, Anouk T.N. van Diepen, Kenan Ates, Daniele Ciurlino, Tolga Yildirim, Christiaan L. Meuwese, Friedo W. Dekker, Hatice Keles, Alattin Kali, James Chess, Oktay Karatan, Jonaz Ripsweden, Zafer Ercan, Peter Bárány, Bengt Lindholm, Marc Dorval, Sadie van Esch, Michael J. Bankart, Seyit Ibrahim Akdag, Ayhan Haspulat, Hyunjin Noh, Ozgur Merhametsiz, Abdul Rashid Qureshi, Andrea Stucchi, Claudio Musetti, Raymond T. Krediet, Mehmet Buyukbakkal, Peter Stenvinkel, Jun Young Do, Zeynep Kendi Celebi, Nicholas Topley, Iván Cabezas-Rodríguez, Mark Lambie, Baris Eser, Dick G. Struijk, Mehmet Deniz Ayli, Bulent Erdogan, Silvio Bertoli, and Hi Bahl Lee

Subjects
Transplantation, medicine.medical_specialty, Nephrology, business.industry, medicine.medical_treatment, Urology, medicine, business, Peritoneal dialysis
Published
2013

19. Renal function and outcome in patients with stable coronary artery disease undergoing coronary angiography. Data from 6 years of consecutive patients in a nationwide registry

Author

Stefan James, J-J Carrero-Roig, Bo Lagerqvist, Tomas Jernberg, Matthew Evans, Robert Edfors, Karolina Szummer, and Jonas Spaak

Subjects
medicine.medical_specialty, Creatinine, business.industry, medicine.medical_treatment, Renal function, Revascularization, medicine.disease, Comorbidity, Coronary artery disease, chemistry.chemical_compound, chemistry, Internal medicine, Diabetes mellitus, Epidemiology, medicine, Cardiology, Myocardial infarction, Cardiology and Cardiovascular Medicine, business
Abstract

Renal function and outcome in patients with stable coronary artery disease undergoing coronary angiography. Data from 6 years of consecutive patients in a nationwide registry

Published
2013

20. Epidemiology & outcome in CKD 5D (1)

Author

W. Winkelmayer, J. Liu, A. Brookhart, H.-Y. Wang, W.-C. Kan, C.-C. Chien, T.-C. Fang, H.-F. Lin, Y.-H. Li, C.-H. Wang, C.-L. Chou, M. Yazawa, Y. Shibagaki, K. Kimura, S. Ohira, K. Ryo, T. Hasegawa, N. Hanafusa, Y. Tsubakihara, K. Iseki, H.-Y. Chen, I.-C. Cheng, Y.-J. Pan, Y.-L. Chiu, S.-P. Hsu, M.-F. Pai, J.-Y. Yang, Y.-S. Peng, T.-J. Tsai, K.-D. Wu, P. Dzekova-Vidimliski, G. Severova-Andreevska, S. Pavlevska, L. Trajceska, G. Selim, S. Gelev, A. Sikole, M. Hecking, A. Karaboyas, R. Saran, A. Sen, M. Inaba, W. H. Horl, R. Pisoni, B. Robinson, G. Sunder-Plassmann, F. K. Port, S. Chiroli, L. Perrault, D. Mitchell, C. Mattin, R. Krause, H. J. Roth, H.-J. Schober-Halstenberg, G. Edenharter, U. Frei, R. Wilson, M. Adena, P. Hodgkins, M. Keith, M. Smyth, C. Couchoud, R. Galland, N.-k. Man, J. Chanliau, V. Lemaitre, J. Traeger, G. von Gersdorff, O. Vega, M. Schaller, L. Usvyat, N. Levin, C. Barth, P. Kotanko, L. Rosales, S. Thijssen, H. Schmid, H. Schiffl, A. Romanos, S. Lederer, K. H. Chu, B. Lam, C. Tang, S. Wong, A. Cheuk, K. F. Yim, H. L. Tang, W. Lee, K. S. Fung, H. Chan, T. K. Ng, K. L. Tong, M. Doyle, A. Severn, J. Traynor, W. Metcalfe, J. Boyd, S. Cairns, J. Reilly, A. Henderson, K. Simpson, D. Tovbin, A. Douvdevani, V. Novack, A. Abd Elkadir, M. Zlotnik, Z. Djuric, N. Dimkovic, J. Popovic, Y. Furumatsu, S. Yamazaki, Y. Hayashino, M. Takegami, Y. Yamamoto, N. Kakudate, T. Wakita, T. Akizawa, T. Akiba, A. Saito, K. Kurokawa, S. Fukuhara, G. Voronovitsky, L. Pinelli, L. Paganti, J. Silva, R. Garofalo, E. Reiss, J. Gimenez Torrado, P. Lafroscia, M. Lugo, S. Laplante, P. Vanovertveld, M. Nordio, A. Limido, U. Maggiore, M. Nichelatti, M. Postorino, G. Quintaliani, L. Ebah, D. Kanigicherla, M. Nikam, G. Dutton, S. Mitra, L. Attipoe, J. Baharani, G. Magrini, A. Martorell, Y. Mashima, T. Konta, K. Kudo, K. Suzuki, A. Ikeda, S. Takasaki, I. Kubota, J. Chudek, K. Wieczorowska-Tobis, A. Wiecek, null Members of the \\'PolSenior\\' Study Group, J. M. des Grottes, F. Collart, H. Maheut, D. A. Goodkin, B. Bieber, B. M. Robinson, M. Jadoul, M. Djogan, I. Dudar, T. Sergeyeva, K. Yamagata, H. Nishi, S. Nishi, K. Hommel, M. Madsen, T. M. Blicher, A.-L. Kamper, I. Masakane, S. Ito, M. Seino, M. Ito, J. Nagasawa, H. C. Rayner, D. S. Fuller, B. W. Gillespie, H. Morgenstern, F. Tentori, R. L. Pisoni, J.-J. Wang, J.-C. Hwang, D. Mladenovska, G. Severova, V. Amitov, P. Yadav, J. J. Carrero, D. J. Jager, M. Verduijn, P. Ravani, J. De Meester, J. G. Heaf, P. Finne, A. J. Hoitsma, J. Pascual, F. Jarraya, A. V. Reisaeter, F. W. Dekker, K. J. Jager, H. Sammut, M. S. A. Ahmed, J. Sheppard, N. Attwood, G. Cserep, K. Sinnamon, I. Katsipi, A. Tatsiopoulos, C. Doulgerakis, P. Papanikolaou, E. Kardouli, G. Lamprinoudis, K. Kintzoglanakis, M. Gennadiou, J. Kyriazis, A. Granger Vallee, E. Covic, M. Morena, A. Fournier, B. Canaud, D. Bolignano, S. Rastelli, G. Curatola, G. Caridi, R. Tripepi, G. Tripepi, R. Politi, F. Catalano, D. Delfino, M. Ciccarelli, F. Mallamaci, and C. Zoccali

Subjects
Transplantation, medicine.medical_specialty, Nephrology, business.industry, Epidemiology, medicine, Intensive care medicine, business, Outcome (game theory)
Published
2011

21. Treatment of malnutrition and anaemia

Author

Z. Xu, Y. Chen, A. Huang, Z. Varghese, J. Moorhead, S. Powis, Q. Li, X. Ruan, K. M. Espe, J. Raila, A. Henze, V. Krane, F. J. Schweigert, B. Hocher, C. Wanner, C. Drechsler, N. Sahni, K. L. Gupta, R. Prasad, S. V. Rana, A. Bhalla, J. J. Carrero, P. Barany, M. I. Yilmaz, A. R. Qureshi, A. Sonmez, O. Heimburger, T. Ozgurtas, M. Yenicesu, B. Lindholm, P. Stenvinkel, A. Schneider, D. H. Krieter, U. Fraass, M. P. Schneider, K. Leu, R. Mortensen, A. Worth, S. Singh, P. Schatz, P. Young, D. Wojchowski, and J. Green

Subjects
Transplantation, Malnutrition, Pediatrics, medicine.medical_specialty, Nephrology, business.industry, medicine, medicine.disease, business
Published
2011

22. Pathophysiology and clinical studies in CKD 1-5

Author

M. El Sharkawy, K. Elsaeed, M. Kamel, A. Aziz, C. Del Pozo, A. Balk, J. Castello-Banyuls, D. Navarro, B. Pere, C. C. Faura, J. J. Ballesta, N. Rodig, R. Vilalta, J. Hernandez, J. Camacho Diaz, A.- L. Lapeyraque, J. Sherwinter, R. Gruppo, O. Fremont, V. Baudouin, C. Langman, G. D. Simonetti, C. Loirat, P. Muus, C. Legendre, K. Douglas, M. Hourmant, Y. Delmas, M. Herthelius, A. Trivelli, T. Goodship, C. Bedrosian, C. Licht, N. Schlesinger, H.- Y. Lin, M. De Meulemeester, J. Rovensky, G. Krammer, A. Balfour, A. So, J. J. Carrero, A. Sonmez, M. Saglam, P. Stenvinkel, H. Yaman, A. R. Quresi, M. Yenicesu, M. I. Yilmaz, E. McQuarrie, M. Freel, P. Mark, R. Patel, T. Steedman, R. Fraser, H. Dargie, J. Connell, A. Jardine, S. W. Oh, H. J. Chin, K. Y. Na, D. W. Chae, C. Alfieri, S. Vettoretti, C. Cafforio, R. Floreani, C. Bonanomi, G. Danzi, P. Messa, A. Whelton, P. MacDonald, B. Hunt, L. Gunawardhana, E. Rusu, M. Voiculescu, D. Zilisteanu, M. Ecobici, I. Arsenescu, G. Ismail, C. Macarie, D. Chan, A. Irish, G. Watts, G. Dogra, T. Krueger, G. Schlieper, M. Cozzolino, K. U. Eckardt, M. Jadoul, M. Ketteler, K. Leunissen, L. C. Rump, A. Wiecek, R. Westenfeld, R. D. Hilgers, A. H. Mahnken, L. J. Schurgers, J. Floege, M. Onuigbo, N. Onuigbo, F. Trevisani, M. T. Sciarrone Alibrandi, R. Bertini, F. Montorsi, S. Delli Carpini, T. C. Camerota, S. Antoniolli, L. Citterio, M. Querques, L. Merlino, P. Manunta, L. Ebah, J. Morgan, P. Brenchley, S. Mitra, B. Krumme, J. Boehler, T. Mettang, F. Strutz, O. Georginova, S. Rykova, M. Gafarova, K. Smyr, I. Sokolova, T. Krasnova, and L. Kozlovskaya

Subjects
Transplantation, Nephrology
Published
2011

23. Protein-energy wasting, inflammation and oxidative stress

Author

Kanji Shishido, Georgia Antoniadi, Csilla Z Madarász, Luigi Lombardi, Jose Antonio Sanchez Tomero, Milada Kostirova, Evaggelos Kapeleris, P. Barany, Pedro Figueroa, Suresh C. Tiwari, Adam Remport, Mari Nakamura, Carmen Sanchez, Simona Stancu, Stefan Reuter, Philip Bangen, Marie Vinglerova, Ankur Gupta, Shyam Prakash, Christoforos Papadopoulos, Monika Lichodziejewska-Niemierko, L. Nordfors, Mhairi K. Sigrist, Saso Gelev, Vladimir Pusevski, Siamak Ahmadzade, Bolesław Rutkowski, Archit Patel, Katalin Fornadi, Rui Filipe, Tomohito Matsunaga, Ernesto Rocha, Guillermina Barril, Marta Kalousová, A. Barrientos, Tomoyuki Yamakawa, Marta Codognotto, Artemisia Dona, Kuan-Yu Hung, Yoshiki Nishizawa, Theodoros Eleftheriadis, G. Mircescu, Dana Lixandru, Umapati Hegde, Karina Furaz, I. Bobek, Mohan Rajapurkar, Sylvie Dusilová-Sulková, C. Sintimbreanu, Kathrin Koch, F. Coronel, Noriyuki Kato, Olof Heimbürger, Abdul Rashid Qureshi, Kyoko Nagasue, Liliana González-Espinoza, Marianna Zsom, Maryam Sharifian, Tibor Fülöp, All Nephrologists from Normandy, Alberto de Lorenzo, Marta Novak, Oto Mestek, Alireza Hamidian Jahromi, George Ntatsis, L. Cabrerizo, S. Soni, Atin Kumar, Bayram Edemir, Ana Bernardo, Ewa Aleksandrowicz, Mohammadmahdi Sagheb, Senji Okuno, Andrea Malagoli, Antonio C. Cordeiro, Grammati Galaktidou, Maria Lopez Picasso, Hirokazu Honda, Vassilios Liakopoulos, J. J. Carrero, Nozomu Hosaka, Peter Stenvinkel, Yashar Talebi, Ghanbarali Raissjalali, George Fares, Ramin Radmehr, Tomáš Zima, Miguel Medina, M.A. Rubio, Daisuke Sanada, Christopher W. McIntyre, Mar Ruperto, Kenway Ng, Jan T. Kielstein, Istvan Kiss, Sandeep Mahajan, M. Schalling, Eniko Sárváry, Ales Kubena, Makrouhi Sonikian, Dimitrios Poulikakos, Antonio Piccoli, Sanjay K. Agarwal, Enrique Rojas Campos, Effie Ioannidou, J. Axelsson, Athanassia Kokkinari, Jacky Potier, F. Nalesso, Alexandra Scholze, Stavroula Ziakka, M.J. de la Cruz, Ioannis Stefanidis, Masaaki Inaba, Eleftheria Ferentinou, Daniela Mladenovska, Ambar Khaira, Alfonso M. Cueto-Manzano, M.E. Suliman, C. Ronco, John E. Sanderson, Jonas Axelsson, Jorgen Hegbrant, Isabel Dias, Sanaz Shabani, María Elisa Casos, Luciano De Paola, Suhas Lele, Shiho Kojima, Bengt Lindholm, O. Véber, M. de Cal, Ute Neugebauer, Katharina Krueger, Chen-Hua Liu, Martin Giorgi, Kiyoshi Maekawa, Kaori Kohno, A.R. Qureshi, Laura Cortés-Sanabria, Benjamín Gómez, Ioannis Koutis, Daniel Cruz, Raj Kumar Sharma, Shinichi Mashiba, Giovanni F.M. Strippoli, Bogdan Manolescu, Mohammadreza Khalilzade, M. Rahsaz, Detlef Lang, Anja Juehling, Gabriella Beko, Anupma Kaul, Jamshid Roozbeh, Stefan Heidenreich, Hung-Bin Tsai, Helen Jefferies, R. Martinescu, Rama Tripathi, J.A. Herrero, Anna Rudas, József Eller, Carolina Batis, Amirali Sohrabpoor, Banibrata Mukhopadhyay, Masashi Ueda, Aleksandar Sikole, Pavlina Dzekova, Nicolas Papagalanis, Vladimir Tesar, Lajos Zsom, Susan Ordaz, Cristina Capusa, László Rosivall, Catherine Wells, Tadao Akizawa, Mei-Tsu Chen, Justyna Golebiewska, Charlotta Wollheim, Mikołaj Majkowicz, Rodolfo Valtuille, Youko Katoh, Fabiola Martin del Campo, Anurag Singh, Alfonso Martn Cueto-Manzano, P. Stenvinkel, O.M. Rathi, Pau-Chung Chen, I. Perez Flores, Adolfo Romeo, Juan Jesus Carrero, Michele Buemi, Wieslawa Lysiak-Szydlowska, Linda Cardona, Dimitra Bimplaki, Patricia Pena, Raha Afshariani, Gabriel Mircescu, P. Matilla, Keiko Takahashi, Liliana Gonzalez Espinoza, Navide Ahmadinajad, Ligia Petrescu, Juana Gonzalez, W.I. Alkrekshi, Isabel Fonseca, Mandy Man-Mei Sea, A. Witasp, Hiroki Suzuki, Gulsen Selim, Davide Bolignano, Andre A. Kaplan, Sophia Trompouki, Stephen G. John, Christopher W.K. Lam, Filippos Karakassis, Michael F. Flessner, Makoto Ishizaki, Iris H.S. Chan, Miklos Z. Molnar, Hamid Tayebi Khosroshahi, Jia-Horng Kao, Sishir Gang, Dimosthenis Vlassopoulos, Maria E. Czira, Makoto Watanabe, Elmer Andrés Fernández, R. Martin, Angela Y.M. Wang, Parin Hedayati, Labrini Takouli, Lada Trajceska, Akos Ujszaszi, Agostino Naso, Hermann Pavenstädt, P. Lentini, Ioannis Karabinis, Stefanie M. Bode-Boeger, Peter Bárány, Chara Spiliopoulou, B. Lindholm, Alfonso Martín Cueto Manzano, Jean Woo, Jose Montalban, Anna Dongari-Bagtzoglou, Irina Stoian, Martin Tepel, Giuseppe Coppolino, Kalpesh Gohel, Eiji Ishimura, Istvan Mucsi, Liliana Gonzalez, Roxana Martinescu, Anoop Saraya, Iakovos Skarakis, and Mariana Ayala

Subjects
Transplantation, medicine.medical_specialty, Endocrinology, Nephrology, business.industry, Internal medicine, medicine, Inflammation, Protein energy wasting, medicine.symptom, business, medicine.disease_cause, Oxidative stress
Published
2009

24. Sandfly saliva of Lutzomyia ovallesi (Diptera: Psychodidae) as a possible marker for the transmission of Leishmania in Venezuela Andes region.

Author

Nieves E, Sánchez Y, Sánchez H, Rondón M, González N, and Carrero J

Subjects
Adolescent, Adult, Animals, Female, Humans, Male, Saliva chemistry, Salivary Proteins and Peptides analysis, Venezuela, Antibodies blood, Biomarkers blood, Insect Proteins analysis, Insect Proteins immunology, Psychodidae chemistry
Abstract

Background & Objectives: The saliva of the Phlebotominae is highly immunogenic to the vertebrate host and is a determining factor in the Leishmania infection. The aim of this work was to study the saliva of Lutzomyia ovallesi as a possible risk marker for the transmission of Leishmania., Methods: Two populations of L. ovallesi from different geographical areas and subjected to different environmental conditions were compared by geometric morphometry of the wings, by protein profile analysis of salivary glands and by assessing the presence of anti-saliva protein in human sera confronted with laboratory L. ovallesi saliva., Results: The results showed differences in the isometric size and structure of the wings but no allometric effects. Protein profiles of salivary glands of both the L. ovallesi populations studied were found to be similar, based on 11 protein bands with molecular weights ranging from 16 to 99 kDa. Anti-saliva antibodies were present in human sera, but human sera infected and uninfected with leishmaniasis could not be differentiated., Interpretation & Conclusion: We conclude that the saliva of laboratory-reared L. ovallesi is representative of that of the wild population. It is suggested to study the presence of anti-saliva antibodies in other species of sandflies and mosquitoes.

Published
2012

25. [Retrorectal cystic hamartoma (tailgut cyst): radiologic and pathologic findings].

Author

Cuartero V, López J, Carrero J, and Nevado M

Subjects
Adult, Female, Hamartoma diagnostic imaging, Humans, Magnetic Resonance Imaging, Pregnancy, Pregnancy Complications diagnostic imaging, Rectal Neoplasms diagnostic imaging, Ultrasonography, Hamartoma diagnosis, Pregnancy Complications diagnosis, Rectal Neoplasms diagnosis
Abstract

Cystic hamartoma is a rare congenital lesion that presents as a presacral mass. It arises from remnants of the embryonic postanal gut. It is more common in women and usually presents in middle age. It is usually asymptomatic. It is difficult to distinguish the imaging appearance of cystic hamartoma from that of many other presacral cysts; therefore, histologic analysis is essential for the definitive diagnosis. Complete surgical excision is indicated to establish the diagnosis and avoid complications (infection and malignant transformation).

Published
2008
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26. Glycerol 3-phosphate inhibits swarming and aggregation of Myxococcus xanthus.

Author

Moraleda-Muñoz A, Carrero-Lérida J, Extremera AL, Arias JM, and Muñoz-Dorado J

Subjects
Cloning, Molecular, Gene Expression Regulation, Bacterial, Gene Expression Regulation, Enzymologic, Membrane Transport Proteins genetics, Molecular Sequence Data, Morphogenesis drug effects, Movement drug effects, Myxococcus xanthus drug effects, Phenotype, Glycerophosphates pharmacology, Membrane Transport Proteins metabolism, Movement physiology, Myxococcus xanthus physiology
Abstract

We have cloned a gene of Myxococcus xanthus with similarities to the permease for glycerol 3-phosphate (G3P) of other bacteria. Expression of the gene increased significantly during the first hours of starvation. Swarming of the wild-type strain was inhibited and aggregation was delayed by G3P. Conversely, a DeltaglpT strain aggregated even on rich medium. These results indicate that G3P may function to regulate the timing of aggregation in M. xanthus.

Published
2001
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27. Two-photon fluorescence lifetime imaging microscopy of macrophage-mediated antigen processing.

Author

French T, So PT, Weaver DJ Jr, Coelho-Sampaio T, Gratton E, Voss EW Jr, and Carrero J

Subjects
Animals, Blotting, Western, Cells, Cultured, Dextrans immunology, Dextrans metabolism, Endocytosis, Endopeptidases metabolism, Enzyme-Linked Immunosorbent Assay, Flow Cytometry, Image Processing, Computer-Assisted, Mice, Peptides analysis, Peptides chemical synthesis, Polylysine immunology, Polylysine metabolism, Serum Albumin, Bovine immunology, Serum Albumin, Bovine metabolism, Vacuoles immunology, Vacuoles metabolism, Antigen Presentation, Macrophages immunology, Microscopy, Fluorescence methods
Abstract

Two-photon fluorescence lifetime imaging microscopy was used noninvasively to monitor a fluorescent antigen during macrophage-mediated endocytosis, intracellular vacuolar encapsulation, and protease-dependent processing. Fluorescein-conjugated bovine serum albumin (FITC-BSA) served as the soluble exogenous antigen. As a relatively nonfluorescent probe in the native state, the antigen was designed to reflect sequential intracellular antigen processing events through time-dependent changes in fluorescence properties. Using two-photon lifetime imaging microscopy, antigen processing events were monitored continuously for several hours. During this time, the initial fluorescein fluorescence lifetime of 0.5 ns increased to approximately 3.0 ns. Control experiments using fluorescein conjugated poly-L-lysine and poly-D-lysine demonstrated that the increase in fluorescence parameters observed with FITC-BSA were due to intracellular proteolysis since addition of the inert D-isomer did not promote an increase in fluorescence lifetime or intensity. Comparisons of intravacuolar and extracellular FITC-dextran concentration suggested active localization of dextran in the vacuoles by the macrophage. In addition, the kinetics of degradation observed using two-photon microscopy were similar to results obtained on the flow cytometer, thus validating the use of flow cytometry for future studies.

Published
1997
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28. Anti-metatype antibody stabilization of Fv 4-4-20 variable domain dynamics.

Author

Carrero J, Mallender WD, and Voss EW Jr

Subjects
Animals, Antibodies chemistry, Antibodies, Monoclonal chemistry, Binding Sites, Antibody, Cricetinae, Fluoresceins, Kinetics, Ligands, Mice, Recombinant Proteins chemistry, Recombinant Proteins immunology, Spectrometry, Fluorescence, Antibodies immunology, Antibodies, Monoclonal immunology
Abstract

Anti-metatype (anti-Met) antibodies are immunoglobulins that specifically recognize and stabilize antibodies in their liganded or metatypic state, but lack specificity for either the hapten or the unliganded antibody. Autologous anti-Met antibodies were previously observed in vivo, suggesting that a metatypic autoantibody response could play a physiological role in the immune network, e.g. controlling the clearance of immune complexes from circulation. The first elicited anti-Met antibodies were against the fluorescein-liganded high affinity murine anti-fluorescein monoclonal antibody 4-4-20. The fluorescein-hapten system has proved to be an invaluable tool for both the recognition and characterization of the metatypic response by utilization of its spectral properties. In this investigation, hydrostatic pressure measurements, in conjunction with fluorescence spectroscopy, were performed on the recombinant Fv derivative (Fv 4-4-20) of the high affinity anti-fluorescein monoclonal antibody 4-4-20 complexed to anti-Met antibodies to study the influence of anti-Met antibodies of Fv 4-4-20 intervariable domain interactions. Anti-Met antibodies bound to liganded Fv 4-4-20 were observed to cause a change in the fluorescence properties of fluorescein that was not observed when anti-Met antibodies were bound to the liganded parent immunoglobulin. The variation of these spectral properties upon addition of anti-Met antibodies was shown to be correlated with dissociation of the variable domains in Fv 4-4-20 in response to its interaction with the anti-Met antibody. The ability to cause variable domain dissociation was dependent on whether monoclonal or polyclonal anti-Met antibodies were bound to the metatype. A model was proposed that elucidated the interaction of anti-Met antibodies, polyclonal and monoclonal, with variable domains of the primary anti-antigen antibody.

Published
1996
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29. Temperature and pH dependence of fluorescein binding within the monoclonal antibody 9-40 active site as monitored by hydrostatic pressure.

Author

Carrero J and Voss EW Jr

Subjects
Antibodies, Monoclonal metabolism, Binding Sites, Fluorescein, Fluoresceins metabolism, Fluorescence Polarization, Fluorescent Dyes metabolism, Hydrogen-Ion Concentration, Hydrostatic Pressure, Iodides, Kinetics, Ligands, Mathematics, Models, Theoretical, Protein Conformation, Temperature, Thermodynamics, Antibodies, Monoclonal chemistry, Fluoresceins chemistry, Fluorescent Dyes chemistry
Abstract

In a comparative study, the thermodynamic parameter, DeltaV, was obtained using hydrostatic pressure-induced dissociation of fluorescein (Fl) from the active site of monoclonal antibody (mAb) 9-40 and its mutant and native derivatives equilibrated at six pH values (8.0, 7.5, 7.0, 6.5, 6.0, and 5.5) and four temperatures (35, 25, 15, and 5 degrees C). mAb 9-40 and its Fab and single-chain Fv (scFv) derivatives at pH 8.0 were found to have identical Fl dissociation behavior under pressure as a function of temperature. The pressure dissociation at 25 degrees C as a function of pH showed a sigmoidal dependence of DeltaV with a midpoint value at pH 7.4 for mAb 9-40. Comparison of experimental results for scFv 9-40/212 with its mutant scFv 9-40/212Arg-34L indicated that the pH dependence of mAb 9-40 was due to the titration of His-34L in the active site. Iodide quenching of bound Fl showed that the hapten in this active site was solvent accessible. Imperfect packing, which leads to increased conformational dynamics, was determined as a possible cause of the low affinity for mAb 9-40.

Published
1996
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30. Comparative properties of the single chain antibody and Fv derivatives of mAb 4-4-20. Relationship between interdomain interactions and the high affinity for fluorescein ligand.

Author

Mallender WD, Carrero J, and Voss EW Jr

Subjects
Amino Acid Sequence, Animals, Antibodies, Monoclonal biosynthesis, Bacterial Outer Membrane Proteins biosynthesis, Bacterial Outer Membrane Proteins chemistry, Binding Sites, Antibody, Circular Dichroism, Cloning, Molecular, Enzyme-Linked Immunosorbent Assay, Escherichia coli, Fluorescein, Immunoglobulin Fab Fragments chemistry, Immunoglobulin G biosynthesis, Kinetics, Mice, Mice, Inbred BALB C, Models, Structural, Molecular Sequence Data, Protein Conformation, Recombinant Proteins biosynthesis, Recombinant Proteins chemistry, Structure-Activity Relationship, Antibodies, Monoclonal chemistry, Fluoresceins, Fluorescent Dyes, Immunoglobulin G chemistry, Immunoglobulin Light Chains chemistry, Immunoglobulin Variable Region chemistry
Abstract

Recombinant Fv derivative of the high affinity murine anti-fluorescein monoclonal antibody 4-4-20 was constructed and expressed in high yields, relative to the single chain antibody (SCA) derivative (2 3-fold), in Escherichia coli. Both variable heavy (VH) and variable light (VL) domains, that accumulated as insoluble inclusion bodies, were isolated, denatured, mixed, refolded, and affinity-purified to yield active Fv 4-4-20. Affinity-purified Fv 4-4-20 showed identical ligand binding properties compared with the SCA construct, both were slightly lower than the affinities expressed by Fab or IgG 4-4-20. Proper protein folding was shown to be domain-independent by in vitro mixing of individually refolded variable domains to yield functional Fv protein. In solid phase and solution phase assays, Fv 4-4-20 closely approximated the SCA derivative in terms of both idiotype and metatype, confirming identical active site structures and conformations. The equilibrium dissociation constant (Kd) for the VL/VH association (1.43 x 10(-7) M), which was determined using the change in fluorescein spectral properties upon ligand binding, was relatively low considering the high affinity displayed by the Fv protein for fluorescein (Kd, 2.9 x 10(-10) M). Thus, domain-domain stability in the Fv and SCA 4-4-20 proteins cannot be the sole cause of reduced affinity (2-3-fold) for fluorescein as compared with the Fab or IgG form of 4-4-20. With their identical ligand binding and structural properties, the decreased SCA or Fv affinity for fluorescein must be an ultimate consequence of deletion of the CH1 and CL constant domains. Collectively, these results verify the importance of constant domain interactions in antibody variable domain structure-function analyses and future antibody engineering endeavors.

Published
1996
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31. Macrophage mediated processing of an exogenous antigenic fluorescent probe: time-dependent elucidation of the processing pathway.

Author

Weaver DJ Jr, Cherukuri A, Carrero J, Coelho-Sampaio T, Durack G, and Voss EW Jr

Subjects
Adenosine Triphosphate biosynthesis, Alkalies pharmacology, Ammonium Chloride pharmacology, Animals, Antigen Presentation drug effects, Cell Line immunology, Flow Cytometry, Fluorescein-5-isothiocyanate, Fluorescence Polarization, Kinetics, Macrophages cytology, Mice, Polymers, Protease Inhibitors pharmacology, Serum Albumin, Bovine immunology, Serum Albumin, Bovine metabolism, Time Factors, Antigen Presentation immunology, Macrophages immunology
Abstract

To elucidate time-dependent pathways and mechanisms involved in antigen processing, a fluorescent probe suitable to monitor several steps within this pathway was developed. Previous studies utilizing two-photon fluorescence microscopy with time resolved and intensity imaging demonstrated that the probe, fluorescein derivatized BSA, was localized to the endocytic system and degraded over an extended period of time. However, an additional method, flow cytometry, was required to monitor the kinetics of these intracellular events and to better assess the total cell population. Flow cytometric studies indicated that the antigen entered an acidic intracellular environment consistent with the endocytic system of the macrophage. Additional experiments suggested that minimal proteolytic degradation began 10 min after addition of the antigenic probe while extensive enzymatic degradation did not occur until 180-200 min. Inhibitor studies indicated that degradation of the probe was dependent upon both acidic pH and ATP synthesis as well as all four classes of proteases. Experiments involving specific protease inhibitors also revealed that various classes of proteases were active at different time points throughout the processing of the probe. By combining these results with additional kinetic data, a model for the sequence of events involved in the processing of FITC10BSA was proposed. More importantly, these studies represented some of the first time-dependent kinetic measurements of antigen processing in living cells.

Published
1996

32. Relative conformational stabilities of single-chain pocket and groove-shaped antibody active sites including HCDR transplant intermediates.

Author

Gulliver GA, Rumbley CA, Carrero J, and Voss EW Jr

Subjects
Antibodies, Monoclonal chemistry, Guanidine, Guanidines chemistry, Protein Conformation, Protein Denaturation, Protein Folding, Recombinant Fusion Proteins chemistry, Spectrometry, Fluorescence, Binding Sites, Antibody, Immunoglobulin Heavy Chains chemistry, Immunoglobulin Variable Region chemistry
Abstract

Stability measurements of SCA 04-01/212 (anti-ssDNA) which possesses a groove-shaped active site were performed by Gdn-HCl-induced unfolding, analyzed assuming a simple two-state equilibrium, and expressed as the free energy of unfolding, delta Gn-u. A delta Gn-u of 1.44 +/- 0.13 kcal/mol was determined experimentally for SCA 04-01/212. In addition, the conformational stabilities of HCDR transplants, hybrid antibody molecules resulting from the transplantation of HCDRs from SCA 4-4-20 (anti-fluorescein) into the corresponding regions of 04-01 in all combinations, were determined using the identical protocol applied to SCA 04-01. On the basis of the results of these stability experiments, the HCDR transplants were categorized into three groups, representing low, intermediate, and high stability. Data were discussed in terms of the relationships between structure-function and conformational stability pertaining to the groove-shaped antibody active site of SCA 04-01/212 and the pocket-shaped active site of SCA 4-4-20/212.

Published
1995
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33. Oxygen penetration and diffusion into myoglobin revealed by quenching of zincprotoporphyrin IX fluorescence.

Author

Carrero J, Jameson DM, and Gratton E

Subjects
Chemical Phenomena, Chemistry, Physical, Diffusion, Fluorescence, Kinetics, Mathematical Computing, Models, Chemical, Myoglobin metabolism, Oxygen metabolism, Solvents chemistry, Temperature, Thermodynamics, Viscosity, Myoglobin chemistry, Oxygen chemistry, Protoporphyrins chemistry
Abstract

Oxygen quenching experiments were carried out on zincprotoporphyrin IX reconstituted myoglobin (MbFe-->Zn) at different temperatures and two solvent viscosities. The data were fit to a dynamic model for quenching of fluorophores in protein interiors previously presented (Biophysical J., 45 (1984) 789-794). The parameters associated with the oxygen entry rate (k+), exit rate (k-), and migration rate (chi) in the protein were obtained at six temperatures and two viscosities (1 and 8 cp), along with the activation enthalpies associated with the above rates (k+ and k-). The partition coefficient (alpha) was calculated at each temperature along with the free energy, delta G0, associated with this partition. The rate parameters (k+, k-, chi) and the partition coefficient (alpha) have also been determined for the sample in 40% sucrose (8 cp), to evaluate the effect of bulk solvent viscosities on these values. The steady-state Stern-Volmer quenching plot was calculated using the rate parameters obtained from the analysis (of the dynamic model). Comparison of the Stern-Volmer points obtained using the dynamic model and those obtained experimentally showed excellent agreement.

Published
1995
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