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This article provides insight into an extensive experimental study on applying a material extrusion 3D printing process, commonly referred to as fused deposition modelling (FDM), to the manufacturing of leakproof capsules for macroencapsulating phase change materials (PCM). It aims at providing a solution to the lack of systematic and reliable production of custom PCM macrocapsules. This study presents an experimental research which tested and compared the impact of multiple 3D printing parameters, geometries and materials in the leakproofness of the capsules when submitted to multiple thermal cycles in a climatic chamber. The challenge of encapsulating materials that cycle between liquid and solid state with varying densities and thus cyclical inner pressure oscillations and mechanical fatigue is overcome. Promising results are shown regarding the encapsulation of organic and inorganic PCMs with both PETG (polyethylene terephthalate glycol) and TPU (thermoplastic polyurethane) polymers, whose capsules withstood 35 melting and freezing cycles. Initial PETG capsules lost close to 50 % of their inner PCM content. With the successive improvements made in each iteration, concerning parameter adjustment, the final cylindrical PETG capsules achieved 0 % PCM mass losses. TPU cylindrical capsules surpassed expectations, achieving 100 % retained PCM at first try, proving that the principles behind the leakproofness of the capsules are transversal across polymers. Layer height, extrusion factor and multiplier, cooling intensity, capsule geometry and polymer material have shown to be the most impactful parameters in the leakproofness of the macrocapsules. Parameters such as the PCM infill percentage managed to reduce leakage up to 94 %, and changes in design (rectangular vs cylindrical capsules) had a reduction in leakage of 44 %.

Abstract Sustainable farming of fish species depends on emerging new feed ingredients, which can alter the features of the digestive tract and influence animals’ overall health. Recent research has shown that functional feeds hold great potential for enhancing fish robustness by evoking appropriate responses at the intestine level. However, there is a lack of extensive and accurate descriptions of the morphology of the gastrointestinal tract of most farmed fish. We have characterised the intestine of European seabass thoroughly, by targeting four segments − anterior, mid, posterior and rectum. Results indicated that the anterior segment is mostly associated with absorption-related features; this segment has the largest absorptive area, the longest villi, and the highest number of neutral goblet cells (GC). The posterior segment and rectum have distinct histomorphometric features, but both seem to be important for immunity, displaying the highest count of acid GC and the highest expression of immune-related genes. The strongest proliferating cell nuclear antigen (PCNA) signal was observed in the anterior intestine and rectum, with PCNA+ cells appearing at the base of the villi and the corresponding villi branches. We have also evaluated the impact of a novel feed supplemented with a macro- and microalgae blend and found that there were no differences in terms of growth. However, the alterations observed in the mid intestine of fish fed the blend, such as thickening of the submucosa and lamina propria, an increased number of leucocytes, and higher expression of immune- and oxidative stress-related genes, suggest that algae may have an immunomodulatory effect. In the current article, we have described the morphology and expression patterns of the intestine segments of European seabass in detail and have presented a comprehensive report of the indices and methods used for the semi-quantitative and quantitative histomorphometric assessments, thereby providing useful information for future studies that aim to maintain intestinal health through dietary interventions.

This paper introduces an experimental setup for retrieving horizontal wind speed and direction profiles with a high temporal and vertical resolution for process studies and validation of convection-permitting model simulations. The CMTRACE (tracing convective momentum transport in complex cloudy atmospheres) campaign used collocated wind lidar and cloud radar measurements to retrieve seamless wind profiles from near the surface up to cloud tops. It took place in Cabauw, the Netherlands, between 13 September and 3 October 2021. The intermediate processing steps for generating the level 1 and level 2 data, such as second trip echoes filtering, offset correction, wind retrieval, re-gridding, and flagging, are described. In level 1 (https://doi.org/10.5281/zenodo.6926483, Dias Neto, 2022a), the data from lidar and radars are kept in the original spatial and temporal resolution, while in level 2 (https://doi.org/10.5281/zenodo.6926605, Dias Neto, 2022b), they are regridded to a common spatial and temporal resolution. Statistical analyses of the lidar's and radar's wind speed and direction profiles indicate a correlation higher than 0.95 for both variables. The bias of wind direction and speed calculated between radar's and lidar's observations are 0.24∘ and −0.16 m s−1, respectively. The foreseen initial application of the datasets includes the study of convective momentum transport and its validation in regional weather forecasts and large-eddy simulation hindcasts.

The dendritic growth layer (DGL), defined as the temperature region between −20 and −10 ∘C, plays an important role for ice depositional growth, aggregation and potentially secondary ice processes. The DGL has been found in the past to exhibit specific observational signatures in polarimetric and vertically pointing radar observations. However, consistent conclusions about their physical interpretation have often not been reached. In this study, we exploit a unique 3-months dataset of mid-latitude winter clouds observed with vertically pointing triple-frequency (X-, Ka-, W-band) and polarimetric W-band Doppler radars. In addition to standard radar moments, we also analyse the multi-wavelength and polarimetric Doppler spectra. New variables, such as the maximum of the spectral differential reflectivity (ZDR) (sZDRmax), allows us to analyse the ZDR signal of asymmetric ice particles independent of the presence of low ZDR producing aggregates. This unique dataset enables us to investigate correlations between enhanced aggregation and evolution of small ice particles in the DGL. For this, the multi-frequency observations are used to classify all profiles according to their maximum average aggregate size within the DGL. The strong correlation between aggregate class and specific differential phase shift (KDP) confirms the expected link between ice particle concentration and aggregation. Interestingly, no correlation between aggregation class and sZDRmax is visible. This indicates that aggregation is rather independent of the aspect ratio and density of ice crystals. A distinct reduction of mean Doppler velocity in the DGL is found to be strongest for cases with largest aggregate sizes. Analyses of spectral edge velocities suggest that the reduction is the combined result of the formation of new ice particles with low fall velocity and a weak updraft. It appears most likely that this updraft is the result of latent heat released by enhanced depositional growth. Clearly, the strongest correlations of aggregate class with other variables are found inside the DGL. Surprisingly, no correlation between aggregate class and concentration or aspect ratio of particles falling from above into the DGL could be found. Only a weak correlation between the mean particle size falling into the DGL and maximum aggregate size within the DGL is apparent. In addition to the correlation analysis, the dataset also allows study of the evolution of radar variables as a function of temperature. We find the ice particle concentration continuously increasing from −18 ∘C towards the bottom of the DGL. Aggregation increases more rapidly from −15 ∘C towards warmer temperatures. Surprisingly, KDP and sZDRmax are not reduced by the intensifying aggregation below −15 ∘C but rather reach their maximum values in the lower half of the DGL. Also below the DGL, KDP and sZDRmax remain enhanced until −4 ∘C. Only there, additional aggregation appears to deplete ice crystals and therefore reduce KDP and sZDRmax. The simultaneous increase of aggregation and particle concentration inside the DGL necessitates a source mechanism for new ice crystals. As primary ice nucleation is expected to decrease towards warmer temperatures, secondary ice processes are a likely explanation for the increase in ice particle concentration. Previous laboratory experiments strongly point towards ice collisional fragmentation as a possible mechanism for new particle generation. The presence of an updraft in the temperature region of maximum depositional growth might also suggest an important positive feedback mechanism between ice microphysics and dynamics which might further enhance ice particle growth in the DGL.

Equatorial Kelvin and mixed Rossby–gravity (MRG) waves in the tropical tropopause layer and stratosphere represented in recent reanalyses for the period of 1981–2010 are compared in terms of spectral characteristics, spatial structures, long-term variations, and their forcing of the quasi-biennial oscillation (QBO). For both wave types, the spectral distributions are broadly similar among most of the reanalyses, while the peak amplitudes exhibit considerable spread. The longitudinal distributions and spatial patterns of wave perturbations show reasonable agreement between the reanalyses. A few exceptions to the similarity of the spectral shapes and spatial structures among them are also noted. While the interannual variations of wave activity appear to be coherent for both the Kelvin and MRG waves, there is substantial variability in long-term trends among the reanalyses. Most of the reanalyses which assimilate satellite data exhibit large increasing trends in wave variance (∼15 %–50 % increase in 30 years at 100–10 hPa), whereas one reanalysis (Japanese 55-year Reanalysis assimilating conventional observations only; JRA-55C) produced without satellite data does not. Several discontinuities are found around 1998 in the time series of the Kelvin and MRG wave variances, which manifest in different ways depending on the reanalysis, and are indicative of impacts of the transition of satellite measurements during that year. The equatorial wave forcing of the QBO, estimated by the Eliassen–Palm (EP) flux divergence, occurs in similar phase-speed ranges in the lower stratosphere among the reanalyses. However, the EP flux and its divergence are found to be dependent on the zonal-mean winds represented in reanalyses, exhibiting different magnitudes, altitudes, and phase-speed ranges of the Kelvin wave forcing between the reanalyses, especially at 20–10 hPa. In addition, at around 20 hPa, a wave signal which appears only in easterly mean winds with westward phase speeds is found and discussed.

This paper describes a 2-month dataset of ground-based triple-frequency (X, Ka, and W band) Doppler radar observations during the winter season obtained at the Jülich ObservatorY for Cloud Evolution Core Facility (JOYCE-CF), Germany. All relevant post-processing steps, such as re-gridding and offset and attenuation correction, as well as quality flagging, are described. The dataset contains all necessary information required to recover data at intermediate processing steps for user-specific applications and corrections (https://doi.org/10.5281/zenodo.1341389; Dias Neto et al., 2019). The large number of ice clouds included in the dataset allows for a first statistical analysis of their multifrequency radar signatures. The reflectivity differences quantified by dual-wavelength ratios (DWRs) reveal temperature regimes where aggregation seems to be triggered. Overall, the aggregation signatures found in the triple-frequency space agree with and corroborate conclusions from previous studies. The combination of DWRs with mean Doppler velocity and linear depolarization ratio enables us to distinguish signatures of rimed particles and melting snowflakes. The riming signatures in the DWRs agree well with results found in previous triple-frequency studies. Close to the melting layer, however, we find very large DWRs (up to 20 dB), which have not been reported before. A combined analysis of these extreme DWR with mean Doppler velocity and a linear depolarization ratio allows this signature to be separated, which is most likely related to strong aggregation, from the triple-frequency characteristics of melting particles.

Sunscreens have been employed on daily skin care for centuries. Their role in protecting the skin from sun damage, avoiding accelerated photoaging and even limiting the risk of development of skin cancer is unquestionable. Although several chemical and physical filters are approved as sunscreens for human use, their safety profile is dependent on their concentration in the formulation which governs their acceptance by the regulatory agencies. A strategic delivery of such molecules should provide a UV protection and limit the skin penetration. Solid lipid nanoparticles (SLN) and nanostructured lipid carriers (NLC) may offer an alternative approach to achieve a synergistic effect on the UV protection when loaded with sunscreens as particles themselves also have a UV light scattering effect. Besides, the lipid character of SLN and NLC improves the encapsulation of lipophilic compounds, with enhanced loading capacity. Silica nanoparticles have also been employed in sunscreen formulations. Due to the formed sol-gel complexes, which covalently entrap sunscreen molecules, a controlled release is also achieved. In the present work, we have developed a new sunscreen formulation composed of hybrid SLN-Silica particles loaded with octyl methoxycinnamate (Parsol®MCX), and their further incorporation into a hydrogel for skin administration. Hybrid SLN-silica particles of 210.0 ± 3.341 nm of mean size, polydispersity below 0.3, zeta potential of ca. |7| mV, loading capacity of 19.9% and encapsulation efficiency of 98.3% have been produced. Despite the slight negative surface charge, the developed hybrid nanoparticles remained physicochemically stable over the study period. Turbiscan transmission profiles confirmed the colloidal stability of the formulations under stress conditions. The texture profile analysis of Parsol-SLN and Parsol-SLN-Si revealed semi-solid properties (e.g. adhesiveness, hardness, cohesiveness, springiness, gumminess, chewiness, resilience) suitable for topical application, together with the bioadhesiveness in the skin of pig ears. The non-irritation profile of the hybrid nanoparticles before and after dispersion into Carbopol hydrogels was confirmed by HET-CAM test.

Este trabalho se constituiu em um estudo de ação-reflexão sobre a prática pedagógica de Educação Ambiental (EA) desenvolvida no contexto do ensino de Química na Escola Municipal Monsenhor Stanislaw localizada no município de Olivedos-PB. O objetivo da pesquisa foi desenvolver e analisar ações pedagógicas de inserção de práticas educativas ambientais articuladas com o ensino de Química, com a implantação de coleta seletiva na escola, oficinas de reciclagens tais como a de papel reciclado, óleo utilizado em frituras para fabricação de sabão e reutilização de garrafas PET´S. Para a reflexão sobre a prática vivenciada, foi feita uma coleta de dados, por meio de registro de observações em diário de campo, aplicação de questionários, fotografias como documentos visuais, realização de várias oficinas de caráter educacional relacionado ao meio ambiente. As ações desenvolvidas consistiram na abordagem de temas ambientais por meio do livro didático; no desenvolvimento de um projeto de Educação Ambiental para identificar problemas ambientais em volta da escola como também na cidade em geral. Pôde-se observar a importância do uso do livro didático, mas também evidencia a necessidade do desenvolvimento de projetos de ações comunitárias de educação ambiental (EA) e de projetos que envolvam a comunidade escolar. Constatou-se que a EA no ensino de Química requer um processo longo de construção de ações coletivas, em que alunos e professores sejam sensibilizados e mobilizados para se engajarem em ações socioambientais. Isso significa construir um processo contínuo de renovar estratégias, recriar ações, dialogar nas aulas, utilizar técnicas para desenvolver no aluno autonomia e criatividade. Nesse sentido, envolver práticas educativas ambientais na comunidade escolar é deixar aflorar os valores, as atitudes, os conceitos e as habilidades para transformá-las em ação com a esperança em criar trilhas para construir uma nova realidade.

The present study defines new interpolation functions for hydrological data. These functions are applied to generate climatological maps of temperature–salinity distribution with a 25 m depth interval and a 30 km space interval (MEDTRANS data set). The data undergo rigorous initial data quality control, having passed several filtering procedures. The gridding is done on neutral density surfaces, which allows better representation of thermohaline fronts for the same gridding radius. The multi-pass Barnes optimum interpolation procedure with spatially variable size of the gridding window is used. The shape of the window accounts for the dominant along-isobath direction of water mass transport over steeply sloping topography. A local ratio of topographic to planetary β-effects is used to define the shape of the window as a function of the relative importance of the topographic influence. The N/f ratio is applied to account for the baroclinic compensation decay of the topographic influence on water mass transport with the distance from the bottom. The gridded fields are available at the website of the Centre of Oceanography of the University of Lisbon (http://co.fc.ul.pt/en/data). The MEDTRANS climatology gives more details of the distribution of water characteristics in the subtropical northeastern Atlantic than other alternative climatologies and is able to reproduce a number of dynamic features described in the literature: the acceleration in the meanders of the Azores current; the cyclonic gyre in the Gulf of Cadiz; and the splitting and separation of the Mediterranean Water (MW) outflow in two veins near the Gorringe and Galicia banks. Seasonal climatologies, computed for the warm (May–October) and cold (November–April) seasons, reveal stronger zonal extension of the upper ocean patterns during the warm season, as compared to the cold one.

Two meddies were identified in the Iberian Basin using shipboard ADCP (Meddy 1) and Argo float (Meddy 2) in contrasting background conditions. Meddy 1 was observed while interacting with the Azores Current (AzC), while Meddy 2 was observed in a much calmer dynamical background, north from the AzC jet. In both cases the meddies formed a clear anticyclonic surface signal, detectable in altimetry as well as in sea-surface temperature (SST). Analysis of the in situ observations of the dynamic signal over Meddy 1 showed that the signal, generated by the moving meddy, dominated the AzC dynamics at least up to the base of the seasonal thermocline even at the late stages of its interaction with the jet. The centre of rotation of the surface signal was shifted south-westward from the axis of the meddy by about 18 km, and its dynamic radius was 2 times bigger than that of the meddy. In the centre of the anticyclonic surface signals of both meddies, SST was colder than that of the surrounding water, in contrast to warm SST anomalies in the cores of surface anticyclones generated by meandering surface currents. The latter difference gives ground for identification of meddies (as well as other sub-surface anticyclones) in comparatively dynamically calm regions using coupled altimetry–SST remote sensing data. An identification of Meddy 1 prior to the shipboard ADCP measurements was the first successful experience. At the same time, SST anomalies over the meddies were rather weak, often unstable and statistically significant only over periods of months.

Resumo: Introduçâo: Em Abril de 2009 o Centers for Disease Control and Prevention anunciou a identificação de um novo vírus influenza em dois doentes na Califórnia, o qual viria a ser designado como influenza A (H1N1) 2009. No dia 11 de Junho de 2009 o Director-Geral da Organização Mundial de Saúde declarou estarmos perante uma pandemia por vírus influenza A (H1N1). Em Portugal o primeiro caso de gripe por vírus influenza A (H1N1) foi registado no dia 29 de Abril de 2009, tendo na região Norte sido declarado o primeiro caso pouco tempo depois. Objectivos: Neste artigo pretende-se dar uma visão global sobre as características da onda pandémica de Outono/Inverno no Norte de Portugal. Resultados: Registaram-se 64 195 casos de síndrome gripal nos serviços públicos de saúde da região entre a semana 40 de 2009 e a semana 4 de 2010. A taxa acumulada de casos registados foi de 17,1 por mil habitantes. A maior parte dos casos ocorreu no sexo feminino e em menores de 20 anos de idade. O pico da onda de Outono/Inverno foi atingido na semana 48, mas a distribuição geográfica e temporal da onda pandémica foi muito heterogénea na região. A taxa de hospitalização por síndrome gripal na população foi mais elevada nos menores de 10 anos de idade e decresceu nos grupos etários mais velhos. Registaram-se 44 óbitos em casos confirmados de Gripe A na região (taxa de mortalidade â 1,2/100 000) sendo o risco de morrer mais baixo nos grupos etários mais jovens. O pico dos óbitos ocorreu duas semanas mais tarde do que o pico dos casos. Conclusôes: Admitimos que o impacto da onda pandémica de Outono/Inverno no Norte de Portugal foi reduzido. Consideramos, no entanto, a importância de prosseguir e reforçar a vigilân-cia da gripe na região. Abstract: Introduction: In April 2009 the Centers for Disease Control and Prevention announced the identification of a novel influenza virus in two patients in California, called influenza A (H1N1) 2009. On 11 June 2009 the Director-General of the World Health Organization declared a pandemic of influenza A (H1N1). In Portugal the first case of pandemic influenza A (H1N1) was reported on 29th April and the Northern Region of the country registered the first cases soon after that. Aim: This report pretends to give an overview of the characteristics of Autumn/Winter pandemic wave in the North of Portugal. Results: A total of 64 195 cases of influenza-like illness were registered in public health services in the region between week 40, 2009 and week 4, 2010. The cumulative attack rate of those cases was 17.1/1 000 inhabitants. Most of the cases occurred in females and in the under 20 years. The peak of the Autumn/Winter wave was attained in week 48, but geographic and time distribution of the pandemic was heterogeneous in the region. Hospitalization rate for influenza-like illness cases in the population was higher for the under 10 years and decreased with age. Forty four deaths in pandemic influenza A (H1N1) laboratory-confirmed cases occurred in the region (mortality rate â 1.2/100 000) and the risk of death was lower in younger age groups. The peak of deaths occurred two weeks latter than the peak of cases. Conclusion: We can assume that the Autumn/Winter pandemic wave impact was mild in the Northern Region of Portugal. We consider the importance of pursuing and reinforcing influenza surveillance in the region. Palavras-chave: Gripe, H1N1, Pandemia, 2009, Keywords: Influenza, H1N1, Pandemics, 2009

The design of passive damping treatments using viscoelastic materials requires both an accurate numerical analysis approach, usually making use of the finite element method, and a realistic means of material description. While the former has been widely studied and several and valuable approaches have become available during the last years, the latter is still an issue requiring additional efforts. The experimental characterization, the data modeling and finally the constitutive models able to be directly used along analytic and numerical analysis, are still important research areas. Several viscoelastic models, able to be directly applied into a finite element analysis either in a time or in a frequency domain analysis, are available and have been widely used during the last years in most of the published work. Despite the general description and straightforward use that such modeling approaches may provide, temperature effect is usually disregarded and isothermal analysis are usually performed. Moreover, this temperature effect is naturally not directly considered as an input parameter for most of the viscoelastic material models and isothermal conditions are also considered in the experimental characterization data analysis. This work presents an extended viscoelastic model, based on well known isothermal models, where the temperature-frequency superposition effect is directly considered. The extended model is applied to the analysis of the experimental data using a data fitting procedure to identify a set of global parameters able to represent the effect of the frequency and the temperature.

A strategy to obtain functional tissues engineering with desired biomechanical properties was used to develop scaffolds with morphologies mimicking the native environment to guide tissue regeneration. Non-woven scaffolds, with fibre dimensions at a nanometre scale, can mimic the physical structure of natural extracellular matrices (ECM). Though its clinical application is yet limited, nano/micro fibrous scaffolds produced by electrospinning gains more and more interest in different Tissue Engineering fields. The electrospinning technique is controlled by several parameters, such as polymer solution and processing ambient, being one of the most important parameters the solution viscosity, which allows defining the minimum viscosity needed to obtain fibres. This research work investigates the rheological behaviour of PCL solutions to produce nanoscale fibre meshes for cartilage application. Poly (e-caprolactone) (PCL) solutions were prepared using glacial acetic acid (AA) and glacial acetic acid with triethylamine (AA/TEA) at different concentrations. It was necessary to double the value of the critical concentration (c*), that is 10 wt% for PCL/AA and 9,6wt% for PCL/AA/TEA, to prepare suitable fibres. Results also show that a more homogenous mesh can be produced by adding TEA.

Primary cutaneous follicle center lymphoma (PCFCL) is characterized by a proliferation of follicle center cells in the skin. A definitive diagnosis is frequently delayed because of difficulties in interpretation of the histopathologic findings. It has an excellent prognosis with a 5-year survival over 95% and its risk of transformation has not been established. We describe a case report of man with a gastric diffuse large B-cell lymphoma (DLBCL) referred to our clinic because of nodules in the back that had gradually developed over a period of 10 years. A biopsy performed 3 years before was interpreted as reactive follicular hyperplasia. A new skin biopsy revealed a diffuse large B-cell lymphoma and immunoglobulin heavy chain gene rearrangements from the initial skin biopsy (PCBCL) and the DLBCL gastric biopsy were studied by polymerase chain reaction and an identical clonal rearrangement was detected which was highly suggestive of a transformation lymphoma.

This paper presents near-infrared spectropolarimetric and velocimetric observations of the young planet-hosting T Tauri star PDS 70, collected with SPIRou at the 3.6-m Canada–France–Hawaii Telescope from 2020 to 2024. Clear Zeeman signatures from magnetic fields at the surface of PDS 70 are detected in our data set of 40 circularly polarized spectra. Longitudinal fields inferred from Zeeman signatures, ranging from |$-116$| to 176 G, are modulated on a time-scale of |$3.008\pm 0.006$| d, confirming that this is the rotation period of PDS 70. Applying Zeeman–Doppler imaging to subsets of unpolarized and circularly polarized line profiles, we show that PDS 70 hosts low-contrast brightness spots and a large-scale magnetic field in its photosphere, featuring in particular a dipole component of strength 200–420 G that evolves on a time-scale of months. From the broadening of spectral lines, we also infer that PDS 70 hosts a small-scale field of |$2.51\pm 0.12$| kG. Radial velocities derived from unpolarized line profiles are rotationally modulated as well, and exhibit additional longer term chromatic variability, most likely attributable to magnetic activity rather than to a close-in giant planet (with a 3 |$\sigma$| upper limit on its minimum mass of |$\simeq$| 4 at a distance of |$\simeq$| 0.2 au). We finally confirm that accretion occurs at the surface of PDS 70, generating modulated redshifted absorption in the 1083.3-nm He  i triplet, and show that the large-scale magnetic field, often strong enough to disrupt the inner accretion disc up to the corotation radius, weakens as the star gets fainter and redder (as in 2022), suggesting that dust from the disc more easily penetrates the stellar magnetosphere in such phases. [ABSTRACT FROM AUTHOR]

Objective To evaluate whether there is an association between altered maternal lipid profile and the lipid profile of the newborn in a maternity hospital. Subjects and method Cross-sectional study with 435 parturients and their respective newborns. Blood samples from the newborns were collected during delivery by venipuncture of the umbilical cord close to the placenta. Blood samples from the parturients were collected in the pre-delivery room or right after delivery. The concentrations of total cholesterol, triglycerides and HDL-c were determined by an enzymatic colorimetric method and LDL-c was calculated by the Friedewald formula. Results There was no significant difference in mean concentrations of total cholesterol, LDL-c, HDL-c and triglycerides in neonates according to altered or non-altered maternal total cholesterol, LDL-c, HDL-c and triglycerides. Conclusions Change in maternal lipid profile is not significantly associated with the mean concentrations of total cholesterol, LDL-c, HDL-c and triglycerides in newborns.

Background: Several studies have shown that active compounds of Asclepias subulata (cardenolides) have antiproliferative effect on human cancer cells. Cardenolides isolated from A. subulata can be used as active chemical markers to elaborate phytopharmaceutical preparations. The aim of this work was to evaluate the antiproliferative effect of a standardized extract of the aerial parts, based on Asclepias subulata cardenolides. Methods: Four standardized extracts were prepared by HPLC-DAD depending on the concentration of calotropin and the antiproliferative activity was measured for the MTT assay, on the A549, MCF-7, HeLa, PC3 and ARPE cell lines. The concentrations of calotropin used for the standardization of the extracts were 10, 7.6, 5 and 1 mg/dL. Results: Standardization of the A. subulata extract based on calotropin at 7.6 mg/g dry weight was achieved and the antiproliferative activity was evaluated over A549, HeLa and MCF-7 cell lines, obtaining proliferation percentages of 3.8 to 13.4% . Conclusions: The standardized extracts of A. subulata at different concentrations of calotropin showed antiproliferative activity against all the cell lines evaluated. The greatest effect was observed against the HeLa cell line.

Background: Several studies have shown that active compounds of Asclepias subulata (cardenolides) have antiproliferative effect on human cancer cells. Cardenolides isolated from A. subulata can be used as active chemical markers to elaborate phytopharmaceutical preparations. To evaluate the antiproliferative effect of a standardized extract of the aerial parts, based on Asclepias subulata cardenolides. Methods: Four standardized extracts were prepared by HPLC-DAD depending on the concentration of calotropin and the antiproliferative activity was measured for the MTT assay, on the A549, MCF-7, HeLa, PC3 and ARPE cell lines. The concentrations of calotropin used for the standardization of the extracts were 10, 7.6, 5 and 1 mg/dL. Results: Standardization of the A. subulata extract based on calotropin at 7.6 mg/g dry weight was achieved and the antiproliferative activity was evaluated over A549, HeLa and MCF-7 cell lines, obtaining proliferation percentages of 3.8 to 13.4% . Conclusions: The standardized extracts of A. subulata at different concentrations of calotropin showed antiproliferative activity against all the cell lines evaluated. The greatest effect was observed against the HeLa cell line.