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6 results on '"J L, Ashurst"'

2. The DNA sequence and comparative analysis of human chromosome 10

Author

P, Deloukas, M E, Earthrowl, D V, Grafham, M, Rubenfield, L, French, C A, Steward, S K, Sims, M C, Jones, S, Searle, C, Scott, K, Howe, S E, Hunt, T D, Andrews, J G R, Gilbert, D, Swarbreck, J L, Ashurst, A, Taylor, J, Battles, C P, Bird, R, Ainscough, J P, Almeida, R I S, Ashwell, K D, Ambrose, A K, Babbage, C L, Bagguley, J, Bailey, R, Banerjee, K, Bates, H, Beasley, S, Bray-Allen, A J, Brown, J Y, Brown, D C, Burford, W, Burrill, J, Burton, P, Cahill, D, Camire, N P, Carter, J C, Chapman, S Y, Clark, G, Clarke, C M, Clee, S, Clegg, N, Corby, A, Coulson, P, Dhami, I, Dutta, M, Dunn, L, Faulkner, A, Frankish, J A, Frankland, P, Garner, J, Garnett, S, Gribble, C, Griffiths, R, Grocock, E, Gustafson, S, Hammond, J L, Harley, E, Hart, P D, Heath, T P, Ho, B, Hopkins, J, Horne, P J, Howden, E, Huckle, C, Hynds, C, Johnson, D, Johnson, A, Kana, M, Kay, A M, Kimberley, J K, Kershaw, M, Kokkinaki, G K, Laird, S, Lawlor, H M, Lee, D A, Leongamornlert, G, Laird, C, Lloyd, D M, Lloyd, J, Loveland, J, Lovell, S, McLaren, K E, McLay, A, McMurray, M, Mashreghi-Mohammadi, L, Matthews, S, Milne, T, Nickerson, M, Nguyen, E, Overton-Larty, S A, Palmer, A V, Pearce, A I, Peck, S, Pelan, B, Phillimore, K, Porter, C M, Rice, A, Rogosin, M T, Ross, T, Sarafidou, H K, Sehra, R, Shownkeen, C D, Skuce, M, Smith, L, Standring, N, Sycamore, J, Tester, A, Thorpe, W, Torcasso, A, Tracey, A, Tromans, J, Tsolas, M, Wall, J, Walsh, H, Wang, K, Weinstock, A P, West, D L, Willey, S L, Whitehead, L, Wilming, P W, Wray, L, Young, Y, Chen, R C, Lovering, N K, Moschonas, R, Siebert, K, Fechtel, D, Bentley, R, Durbin, T, Hubbard, L, Doucette-Stamm, S, Beck, D R, Smith, and J, Rogers

Subjects
Base Composition, Multidisciplinary, Pan troglodytes, Chromosomes, Human, Pair 10, Genetics, Medical, Genetic Variation, Proteins, Exons, Genomics, Sequence Analysis, DNA, Physical Chromosome Mapping, Evolution, Molecular, Contig Mapping, Genes, Gene Duplication, Animals, Humans, CpG Islands, Pseudogenes
Abstract

The finished sequence of human chromosome 10 comprises a total of 131,666,441 base pairs. It represents 99.4% of the euchromatic DNA and includes one megabase of heterochromatic sequence within the pericentromeric region of the short and long arm of the chromosome. Sequence annotation revealed 1,357 genes, of which 816 are protein coding, and 430 are pseudogenes. We observed widespread occurrence of overlapping coding genes (either strand) and identified 67 antisense transcripts. Our analysis suggests that both inter- and intrachromosomal segmental duplications have impacted on the gene count on chromosome 10. Multispecies comparative analysis indicated that we can readily annotate the protein-coding genes with current resources. We estimate that over 95% of all coding exons were identified in this study. Assessment of single base changes between the human chromosome 10 and chimpanzee sequence revealed nonsense mutations in only 21 coding genes with respect to the human sequence.

Published
2004

3. The DNA sequence and analysis of human chromosome 13

Author

A, Dunham, L H, Matthews, J, Burton, J L, Ashurst, K L, Howe, K J, Ashcroft, D M, Beare, D C, Burford, S E, Hunt, S, Griffiths-Jones, M C, Jones, S J, Keenan, K, Oliver, C E, Scott, R, Ainscough, J P, Almeida, K D, Ambrose, D T, Andrews, R I S, Ashwell, A K, Babbage, C L, Bagguley, J, Bailey, R, Bannerjee, K F, Barlow, K, Bates, H, Beasley, C P, Bird, S, Bray-Allen, A J, Brown, J Y, Brown, W, Burrill, C, Carder, N P, Carter, J C, Chapman, M E, Clamp, S Y, Clark, G, Clarke, C M, Clee, S C M, Clegg, V, Cobley, J E, Collins, N, Corby, G J, Coville, P, Deloukas, P, Dhami, I, Dunham, M, Dunn, M E, Earthrowl, A G, Ellington, L, Faulkner, A G, Frankish, J, Frankland, L, French, P, Garner, J, Garnett, J G R, Gilbert, C J, Gilson, J, Ghori, D V, Grafham, S M, Gribble, C, Griffiths, R E, Hall, S, Hammond, J L, Harley, E A, Hart, P D, Heath, P J, Howden, E J, Huckle, P J, Hunt, A R, Hunt, C, Johnson, D, Johnson, M, Kay, A M, Kimberley, A, King, G K, Laird, C J, Langford, S, Lawlor, D A, Leongamornlert, D M, Lloyd, C, Lloyd, J E, Loveland, J, Lovell, S, Martin, M, Mashreghi-Mohammadi, S J, McLaren, A, McMurray, S, Milne, M J F, Moore, T, Nickerson, S A, Palmer, A V, Pearce, A I, Peck, S, Pelan, B, Phillimore, K M, Porter, C M, Rice, S, Searle, H K, Sehra, R, Shownkeen, C D, Skuce, M, Smith, C A, Steward, N, Sycamore, J, Tester, D W, Thomas, A, Tracey, A, Tromans, B, Tubby, M, Wall, J M, Wallis, A P, West, S L, Whitehead, D L, Willey, L, Wilming, P W, Wray, M W, Wright, L, Young, A, Coulson, R, Durbin, T, Hubbard, J E, Sulston, S, Beck, D R, Bentley, J, Rogers, and M T, Ross

Subjects
RNA, Untranslated, Multidisciplinary, Chromosomes, Human, Pair 13, Genes, Genetics, Medical, Chromosome Mapping, Humans, Sequence Analysis, DNA, Physical Chromosome Mapping, Pseudogenes, Article
Abstract

Chromosome 13 is the largest acrocentric human chromosome. It carries genes involved in cancer including the breast cancer type 2 (BRCA2) and retinoblastoma (RB1) genes, is frequently rearranged in B-cell chronic lymphocytic leukaemia, and contains the DAOA locus associated with bipolar disorder and schizophrenia. We describe completion and analysis of 95.5 megabases (Mb) of sequence from chromosome 13, which contains 633 genes and 296 pseudogenes. We estimate that more than 95.4% of the protein-coding genes of this chromosome have been identified, on the basis of comparison with other vertebrate genome sequences. Additionally, 105 putative non-coding RNA genes were found. Chromosome 13 has one of the lowest gene densities (6.5 genes per Mb) among human chromosomes, and contains a central region of 38 Mb where the gene density drops to only 3.1 genes per Mb.

Published
2004

4. Plant expression cassettes for enhanced translational efficiency

Author

M. J. Dowson Day, J. L. Ashurst, and R. A. Dixon

Subjects
Genetics, Translational efficiency, Transgene, fungi, food and beverages, RNA, Plant Science, Biology, Proteomics, biology.organism_classification, Ribosome, Terminator (genetics), Tobacco mosaic virus, Cauliflower mosaic virus, Molecular Biology
Abstract

A number of plant expression cassettes have been constructed that are designed to ensure efficient ribosome recruitment, thereby improving translational efficiency. For this purpose the casettes employ the untranslated leader of the tobacco mosaic virus (TMV) RNA. They also comprise the cauliflower mosaic virus (CaMV) 35 S promoter, with duplicated upstream elements, and the noplaine synthase (nos) terminator. Cloning nests were included 3′ to the TMV leader and 5′ to the promoter to facilitate the construction of transcriptional or translational fusions and promoter modifications. In both a transient assay system and transgenic tobacco these cassettes were found to facilitate highexpression levels of a cytosolic and plastid-targeted reporter protein (β-glucuronidase). Also they enabled the detection of a hitherto very poorly expressed protein.

Published
1994

5. The DNA sequence and analysis of human chromosome 6

Author

A. J. Mungall, S. A. Palmer, S. K. Sims, C. A. Edwards, J. L. Ashurst, L. Wilming, M. C. Jones, R. Horton, S. E. Hunt, C. E. Scott, J. G. R. Gilbert, M. E. Clamp, G. Bethel, S. Milne, R. Ainscough, J. P. Almeida, K. D. Ambrose, T. D. Andrews, R. I. S. Ashwell, A. K. Babbage, C. L. Bagguley, J. Bailey, R. Banerjee, D. J. Barker, K. F. Barlow, K. Bates, D. M. Beare, H. Beasley, O. Beasley, C. P. Bird, S. Blakey, S. Bray-Allen, J. Brook, A. J. Brown, J. Y. Brown, D. C. Burford, W. Burrill, J. Burton, C. Carder, N. P. Carter, J. C. Chapman, S. Y. Clark, G. Clark, C. M. Clee, S. Clegg, V. Cobley, R. E. Collier, J. E. Collins, L. K. Colman, N. R. Corby, G. J. Coville, K. M. Culley, P. Dhami, J. Davies, M. Dunn, M. E. Earthrowl, A. E. Ellington, K. A. Evans, L. Faulkner, M. D. Francis, A. Frankish, J. Frankland, L. French, P. Garner, J. Garnett, M. J. R. Ghori, L. M. Gilby, C. J. Gillson, R. J. Glithero, D. V. Grafham, M. Grant, S. Gribble, C. Griffiths, M. Griffiths, R. Hall, K. S. Halls, S. Hammond, J. L. Harley, E. A. Hart, P. D. Heath, R. Heathcott, S. J. Holmes, P. J. Howden, K. L. Howe, G. R. Howell, E. Huckle, S. J. Humphray, M. D. Humphries, A. R. Hunt, C. M. Johnson, A. A. Joy, M. Kay, S. J. Keenan, A. M. Kimberley, A. King, G. K. Laird, C. Langford, S. Lawlor, D. A. Leongamornlert, M. Leversha, C. R. Lloyd, D. M. Lloyd, J. E. Loveland, J. Lovell, S. Martin, M. Mashreghi-Mohammadi, G. L. Maslen, L. Matthews, O. T. McCann, S. J. McLaren, K. McLay, A. McMurray, M. J. F. Moore, J. C. Mullikin, D. Niblett, T. Nickerson, K. L. Novik, K. Oliver, E. K. Overton-Larty, A. Parker, R. Patel, A. V. Pearce, A. I. Peck, B. Phillimore, S. Phillips, R. W. Plumb, K. M. Porter, Y. Ramsey, S. A. Ranby, C. M. Rice, M. T. Ross, S. M. Searle, H. K. Sehra, E. Sheridan, C. D. Skuce, S. Smith, M. Smith, L. Spraggon, S. L. Squares, C. A. Steward, N. Sycamore, G. Tamlyn-Hall, J. Tester, A. J. Theaker, D. W. Thomas, A. Thorpe, A. Tracey, A. Tromans, B. Tubby, M. Wall, J. M. Wallis, A. P. West, S. S. White, S. L. Whitehead, H. Whittaker, A. Wild, D. J. Willey, T. E. Wilmer, J. M. Wood, P. W. Wray, J. C. Wyatt, L. Young, R. M. Younger, D. R. Bentley, A. Coulson, R. Durbin, T. Hubbard, J. E. Sulston, I. Dunham, J. Rogers, and S. Beck

Subjects
Multidisciplinary, Genes, RNA, Transfer, HLA-B Antigens, Genetic Diseases, Inborn, Animals, Humans, Chromosomes, Human, Pair 6, Exons, Sequence Analysis, DNA, Physical Chromosome Mapping, Pseudogenes
Abstract

Chromosome 6 is a metacentric chromosome that constitutes about 6% of the human genome. The finished sequence comprises 166,880,988 base pairs, representing the largest chromosome sequenced so far. The entire sequence has been subjected to high-quality manual annotation, resulting in the evidence-supported identification of 1,557 genes and 633 pseudogenes. Here we report that at least 96% of the protein-coding genes have been identified, as assessed by multi-species comparative sequence analysis, and provide evidence for the presence of further, otherwise unsupported exons/genes. Among these are genes directly implicated in cancer, schizophrenia, autoimmunity and many other diseases. Chromosome 6 harbours the largest transfer RNA gene cluster in the genome; we show that this cluster co-localizes with a region of high transcriptional activity. Within the essential immune loci of the major histocompatibility complex, we find HLA-B to be the most polymorphic gene on chromosome 6 and in the human genome.

Published
2003

6. Studies of the Potential for Expression of Nitrogenase Fe-Protein in Cells of Higher Plants

Author

J. Watts, J. L. Ashurst, M. J. Dowson-Day, Richard A. Dixon, and M. J. Merrick

Subjects
Translational efficiency, biology, Chemistry, Two-hybrid screening, Nicotiana tabacum, Electroporation, fungi, food and beverages, Nitrogenase, Protoplast, biology.organism_classification, Chloroplast, Biochemistry, Gene
Abstract

The potential for expression of the nitrogenase Fe-protein in Nicotiana tabacum has been examined by construction of hybrid nifH and nifM genes which should target their polypeptide products to the plant chloroplast. In vitro import of these proteins into isolated chloroplasts has been demonstrated both aerobically and anaerobically. Initial attempts to express the engineered genes in transformed plants were unsuccessful, possibly because of the low translational efficiency of the constructs used. Modified plant vectors have now been constructed using viral leader sequences. After electroporation into tobacco mesophyll protoplasts these vectors show significantly improved expression as measured by transient expression assays with s-glucuronidase as the reporter. The improved vectors are now being assessed in transformed plants.

Published
1991

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