1. Differences in cerato-ulmin production between the EAN, NAN and non-aggressive subgroups of Ophiostoma ulmi
- Author
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Wayne C. Richards, C. M. Brasier, Shozo Takai, and J. H. Nordin
- Subjects
Veterinary medicine ,biology ,Toxin ,Plant Science ,Phytotoxin ,Horticulture ,biology.organism_classification ,medicine.disease_cause ,Pathogenicity ,Ulmaceae ,chemistry.chemical_compound ,chemistry ,Ophiostoma ulmi ,Botany ,Genetics ,medicine ,Dutch elm disease ,Mycotoxin ,Agronomy and Crop Science ,Pathogen - Abstract
A test of comparative in vitro cerato-ulmin wilt toxin production in the aggressive and non-aggressive subgroups of the Dutch elm disease pathogen Ophiostoma ulmi was carried out by turbidity and ELISA methods. Ten non-aggressive, ten EAN aggressive and ten NAN aggressive isolates were tested from a range of geographical sources. In liquid shake cultures the non-aggressive isolates produced the greatest and the NAN aggressives the least mean biomass. Despite considerable variation in cerato-ulmin production by individual isolates in three separate experiments, both the turbidity and ELISA methods showed a clear separation of the non-aggressive and aggressive subgroups. Non-aggressive isolates produced little or no cerato-ulmin (ELISA range of means 0–56.0 ng/ml) and EAN and NAN aggressive isolates moderate to high levels (EAN 1.6–89.0 × 104 ng/ml and NAN 0.2–300 × 104 ng/ml). In the aggressive isolates no correlation was detected between cerato-ulmin production and either biomass or pathogenicity to clonal Commelin elm. The role of cerato-ulmin in the pathogenicity of O. ulmi is discussed.
- Published
- 1990
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