1,222 results on '"Jürgen Popp"'
Search Results
2. Raman-Activated, Interactive Sorting of Isotope-Labeled Bacteria
- Author
-
Sepehr Razi, Nicolae Tarcea, Thomas Henkel, Ramya Ravikumar, Aikaterini Pistiki, Annette Wagenhaus, Sophie Girnus, Martin Taubert, Kirsten Küsel, Petra Rösch, and Jürgen Popp
- Subjects
Raman spectroscopy ,microfluidic sorting ,isotope labeling ,bacteria ,Chemical technology ,TP1-1185 - Abstract
Due to its high spatial resolution, Raman microspectroscopy allows for the analysis of single microbial cells. Since Raman spectroscopy analyzes the whole cell content, this method is phenotypic and can therefore be used to evaluate cellular changes. In particular, labeling with stable isotopes (SIPs) enables the versatile use and observation of different metabolic states in microbes. Nevertheless, static measurements can only analyze the present situation and do not allow for further downstream evaluations. Therefore, a combination of Raman analysis and cell sorting is necessary to provide the possibility for further research on selected bacteria in a sample. Here, a new microfluidic approach for Raman-activated continuous-flow sorting of bacteria using an optical setup for image-based particle sorting with synchronous acquisition and analysis of Raman spectra for making the sorting decision is demonstrated, showing that active cells can be successfully sorted by means of this microfluidic chip.
- Published
- 2024
- Full Text
- View/download PDF
3. MICROSTRUCTURAL AND COMPOSITIONAL CHARACTERIZATION OF ROMAN BRONZE COINS FROM KHIRBAT EDH-DHARIH IN JORDAN
- Author
-
Abdulraouf Mayyas, Wassef Al Sekhaneh, Diya Al Fuqara, Ruba Seiseh, Fardous Al-Ajlouny, Zeidoun Al Muheisen, and Jürgen Popp
- Subjects
khirbat edh-dharih ,jordan ,bronze ,coin ,corrosion ,om ,sem-edx ,xrf ,xrd ,atr-ftir ,Prehistoric archaeology ,GN700-890 ,Archaeology ,CC1-960 - Abstract
This study aimed at investigating the chemical and mineralogical compositions of five Roman coins (four copper-based and one silver-based alloys) corrosion products, and explore the topographic and morphological microscopic features of the patinas formed on the surface of the copper-based coins. For this purpose, an interdisciplinary approach to micro-destructive methods—microscopic (OM and SEM), mineralogical (XRD), elemental (XRF and SEM-EDX), and molecular (ATR-FTIR)—was conducted. The results showed that cuprite is the principle patina initially formed on the surface of the copper-based alloys by the redundant interaction with the surrounding environmental burial conditions, which is most likely an oxygenated and moisturized soil. This interaction was also observed in the formation of a secondary patina composed of malachite and azurite, which lately was invaded by the corrosive cycle process (bronze disease) represented by the formation of nantokite, atacamite and paratacamite that affected the cuprite primary patina of the copper-based coins during burial. The silver-based coin also suffered an aggressive attack by oxygen, sulfur and chloride ions during burial and formed oxide, sulfide, and chloride of silver, in addition to the corrosion products of cuprite, atacamite, and carbonate of copper, which is one of the alloying elements of this coin. The findings of this study also show that the copper-based coins were made of quaternary Cu-Sn-Zn-Pb alloy, and the silver-based coin was made of ternary Ag-Cu-Sn alloy. Therefore, the study points out that these coins were suffering from the corrosion phenomenon by the reaction with oxide, sulfide, carbonate, hydroxyl, and chloride ions, which are most likely found in the burial soil and incorporated within the alloy corrosion products. Contamination with Si, Fe, Al, and Ca elements present in the soil was also seen. We recommend protecting these alloys to prevent further degradation that may occur during storage and exposure to the atmosphere after excavation.
- Published
- 2023
4. Design of a Dispersive 1064 nm Fiber Probe Raman Imaging Spectrometer and Its Application to Human Bladder Resectates
- Author
-
Juan David Muñoz-Bolaños, Tanveer Ahmed Shaik, Arkadiusz Miernik, Jürgen Popp, and Christoph Krafft
- Subjects
bladder tumor ,Raman imaging ,1064 nm ,cluster analysis ,Python toolbox ,Technology ,Engineering (General). Civil engineering (General) ,TA1-2040 ,Biology (General) ,QH301-705.5 ,Physics ,QC1-999 ,Chemistry ,QD1-999 - Abstract
This study introduces a compact Raman spectrometer with a 1064 nm excitation laser coupled with a fiber probe and an inexpensive motorized stage, offering a promising alternative to widely used Raman imaging instruments with 785 nm excitation lasers. The benefits of 1064 nm excitation for biomedical applications include further suppression of fluorescence background and deeper tissue penetration. The performance of the 1064 nm instrument in detecting cancer in human bladder resectates is demonstrated. Raman images with 1064 nm excitation were collected ex vivo from 10 human tumor and non-tumor bladder specimens, and the results are compared to previously published Raman images with 785 nm excitation. K-Means cluster (KMC) analysis is used after pre-processing to identify Raman signatures of control, tumor, necrosis, and lipid-rich tissues. Hierarchical cluster analysis (HCA) groups the KMC centroids of all specimens as input. The tools for data processing and hyperspectral analysis were compiled in an open-source Python library called SpectraMap (SpMap). In spite of lower spectral resolution, the 1064 nm Raman instrument can differentiate between tumor and non-tumor bladder tissues in a similar way to 785 nm Raman spectroscopy. These findings hold promise for future clinical hyperspectral Raman imaging, in particular for specimens with intense fluorescence background, e.g., kidney stones that are discussed as another widespread urological application.
- Published
- 2024
- Full Text
- View/download PDF
5. Intestinal epithelial barrier integrity investigated by label-free techniques in ulcerative colitis patients
- Author
-
Elsie Quansah, Elena Gardey, Anuradha Ramoji, Tobias Meyer-Zedler, Bianca Goehrig, Astrid Heutelbeck, Stephanie Hoeppener, Michael Schmitt, Maximillian Waldner, Andreas Stallmach, and Jürgen Popp
- Subjects
Medicine ,Science - Abstract
Abstract The intestinal epithelial barrier, among other compartments such as the mucosal immune system, contributes to the maintenance of intestinal homeostasis. Therefore, any disturbance within the epithelial layer could lead to intestinal permeability and promote mucosal inflammation. Considering that disintegration of the intestinal epithelial barrier is a key element in the etiology of ulcerative colitis, further assessment of barrier integrity could contribute to a better understanding of the role of epithelial barrier defects in ulcerative colitis (UC), one major form of chronic inflammatory bowel disease. Herein, we employ fast, non-destructive, and label-free non-linear methods, namely coherent anti-Stokes Raman scattering (CARS), second harmonic generation (SHG), two-photon excited fluorescence (TPEF), and two-photon fluorescence lifetime imaging (2P-FLIM), to assess the morpho-chemical contributions leading to the dysfunction of the epithelial barrier. For the first time, the formation of epithelial barrier gaps was directly visualized, without sophisticated data analysis procedures, by the 3D analysis of the colonic mucosa from severely inflamed UC patients. The results were compared with histopathological and immunofluorescence images and validated using transmission electron microscopy (TEM) to indicate structural alterations of the apical junction complex as the underlying cause for the formation of the epithelial barrier gaps. Our findings suggest the potential advantage of non-linear multimodal imaging is to give precise, detailed, and direct visualization of the epithelial barrier in the gastrointestinal tract, which can be combined with a fiber probe for future endomicroscopy measurements during real-time in vivo imaging.
- Published
- 2023
- Full Text
- View/download PDF
6. Illuminating the Tiny World: A Navigation Guide for Proper Raman Studies on Microorganisms
- Author
-
Sandra Baaba Frempong, Markus Salbreiter, Sara Mostafapour, Aikaterini Pistiki, Thomas W. Bocklitz, Petra Rösch, and Jürgen Popp
- Subjects
Raman spectroscopy ,bacteria ,single-cell analysis ,sample isolation ,machine learning ,Organic chemistry ,QD241-441 - Abstract
Raman spectroscopy is an emerging method for the identification of bacteria. Nevertheless, a lot of different parameters need to be considered to establish a reliable database capable of identifying real-world samples such as medical or environmental probes. In this review, the establishment of such reliable databases with the proper design in microbiological Raman studies is demonstrated, shining a light into all the parts that require attention. Aspects such as the strain selection, sample preparation and isolation requirements, the phenotypic influence, measurement strategies, as well as the statistical approaches for discrimination of bacteria, are presented. Furthermore, the influence of these aspects on spectra quality, result accuracy, and read-out are discussed. The aim of this review is to serve as a guide for the design of microbiological Raman studies that can support the establishment of this method in different fields.
- Published
- 2024
- Full Text
- View/download PDF
7. Siamese Networks for Clinically Relevant Bacteria Classification Based on Raman Spectroscopy
- Author
-
Jhonatan Contreras, Sara Mostafapour, Jürgen Popp, and Thomas Bocklitz
- Subjects
Siamese networks ,machine learning ,bacteria classification ,Raman spectroscopy ,Organic chemistry ,QD241-441 - Abstract
Identifying bacterial strains is essential in microbiology for various practical applications, such as disease diagnosis and quality monitoring of food and water. Classical machine learning algorithms have been utilized to identify bacteria based on their Raman spectra. However, convolutional neural networks (CNNs) offer higher classification accuracy, but they require extensive training sets and retraining of previous untrained class targets can be costly and time-consuming. Siamese networks have emerged as a promising solution. They are composed of two CNNs with the same structure and a final network that acts as a distance metric, converting the classification problem into a similarity problem. Classical machine learning approaches, shallow and deep CNNs, and two Siamese network variants were tailored and tested on Raman spectral datasets of bacteria. The methods were evaluated based on mean sensitivity, training time, prediction time, and the number of parameters. In this comparison, Siamese-model2 achieved the highest mean sensitivity of 83.61 ± 4.73 and demonstrated remarkable performance in handling unbalanced and limited data scenarios, achieving a prediction accuracy of 73%. Therefore, the choice of model depends on the specific trade-off between accuracy, (prediction/training) time, and resources for the particular application. Classical machine learning models and shallow CNN models may be more suitable if time and computational resources are a concern. Siamese networks are a good choice for small datasets and CNN for extensive data.
- Published
- 2024
- Full Text
- View/download PDF
8. Thiol‐ene Reaction as Reversible Covalent Bond for the Design of Shape‐Memory Polymers
- Author
-
Farhad Shohraty, Julian Hniopek, Josefine Meurer, Stefan Zechel, Michael Schmitt, Jürgen Popp, and Martin D. Hager
- Subjects
(reversible) Michael addition ,reversible covalent polymer networks ,(reversible) thiol‐ene reaction ,shape‐memory polymers ,Materials of engineering and construction. Mechanics of materials ,TA401-492 ,Engineering (General). Civil engineering (General) ,TA1-2040 - Abstract
Abstract Besides a stable phase, shape‐memory polymers require an additional switchable moiety. In addition to thermal transitions and supramolecular interactions, these units can also be based on covalent bonds. Herein, the use of the reversible thiol‐ene reaction as reversible cross‐linker for the design of shape‐memory polymers is demonstrated. A facile route to polymer networks with a thiol‐ene acceptor and a comonomer (butyl methacrylate or 2‐ethylhexyl methacrylate) cross‐linked by dithiols is introduced. The thermal and mechanical properties of the resulting polymers are characterized in detail. Hereby, the polymers feature excellent shape‐memory behavior with fixity and recovery rates above 90%. This study shows that the thiol‐ene cross‐linker can function as both, the stable and the switchable structural moiety rendering the usage of a covalent cross‐linker unnecessary. This partial reversibility can also be proven by temperature‐depending Raman spectroscopy.
- Published
- 2023
- Full Text
- View/download PDF
9. Label-free multimodal imaging of infected Galleria mellonella larvae
- Author
-
Elsie Quansah, Anuradha Ramoji, Lara Thieme, Kamran Mirza, Bianca Goering, Oliwia Makarewicz, Astrid Heutelbeck, Tobias Meyer-Zedler, Mathias W. Pletz, Michael Schmitt, and Jürgen Popp
- Subjects
Medicine ,Science - Abstract
Abstract Non-linear imaging modalities have enabled us to obtain unique morpho-chemical insights into the tissue architecture of various biological model organisms in a label-free manner. However, these imaging techniques have so far not been applied to analyze the Galleria mellonella infection model. This study utilizes for the first time the strength of multimodal imaging techniques to explore infection-related changes in the Galleria mellonella larvae due to massive E. faecalis bacterial infection. Multimodal imaging techniques such as fluorescent lifetime imaging (FLIM), coherent anti-Stokes Raman scattering (CARS), two-photon excited fluorescence (TPEF), and second harmonic generation (SHG) were implemented in conjunction with histological HE images to analyze infection-associated tissue damage. The changes in the larvae in response to the infection, such as melanization, vacuolization, nodule formation, and hemocyte infiltration as a defense mechanism of insects against microbial pathogens, were visualized after Enterococcus faecalis was administered. Furthermore, multimodal imaging served for the analysis of implant-associated biofilm infections by visualizing biofilm adherence on medical stainless steel and ePTFE implants within the larvae. Our results suggest that infection-related changes as well as the integrity of the tissue of G. mellonella larvae can be studied with high morphological and chemical contrast in a label-free manner.
- Published
- 2022
- Full Text
- View/download PDF
10. Optical photothermal infrared spectroscopy with simultaneously acquired Raman spectroscopy for two-dimensional microplastic identification
- Author
-
Julia Sophie Böke, Jürgen Popp, and Christoph Krafft
- Subjects
Medicine ,Science - Abstract
Abstract In recent years, vibrational spectroscopic techniques based on Fourier transform infrared (FTIR) or Raman microspectroscopy have been suggested to fulfill the unmet need for microplastic particle detection and identification. Inter-system comparison of spectra from reference polymers enables assessing the reproducibility between instruments and advantages of emerging quantum cascade laser-based optical photothermal infrared (O-PTIR) spectroscopy. In our work, IR and Raman spectra of nine plastics, namely polyethylene, polypropylene, polyvinyl chloride, polyethylene terephthalate, polycarbonate, polystyrene, silicone, polylactide acid and polymethylmethacrylate were simultaneously acquired using an O-PTIR microscope in non-contact, reflection mode. Comprehensive band assignments were presented. We determined the agreement of O-PTIR with standalone attenuated total reflection FTIR and Raman spectrometers based on the hit quality index (HQI) and introduced a two-dimensional identification (2D-HQI) approach using both Raman- and IR-HQIs. Finally, microplastic particles were prepared as test samples from known materials by wet grinding, O-PTIR data were collected and subjected to the 2D-HQI identification approach. We concluded that this framework offers improved material identification of microplastic particles in environmental, nutritious and biological matrices.
- Published
- 2022
- Full Text
- View/download PDF
11. Real-time molecular imaging of near-surface tissue using Raman spectroscopy
- Author
-
Wei Yang, Florian Knorr, Ines Latka, Matthias Vogt, Gunther O. Hofmann, Jürgen Popp, and Iwan W. Schie
- Subjects
Applied optics. Photonics ,TA1501-1820 ,Optics. Light ,QC350-467 - Abstract
A fiber optic probe-based Raman imaging system with real-time molecular virtual reality data visualization of chemical boundaries for clinical tissue-boundary demarcation.
- Published
- 2022
- Full Text
- View/download PDF
12. Correcting systematic errors by hybrid 2D correlation loss functions in nonlinear inverse modelling.
- Author
-
Thomas G Mayerhöfer, Isao Noda, Susanne Pahlow, Rainer Heintzmann, and Jürgen Popp
- Subjects
Medicine ,Science - Abstract
Recently a new family of loss functions called smart error sums has been suggested. These loss functions account for correlations within experimental data and force modeled data to obey these correlations. As a result, multiplicative systematic errors of experimental data can be revealed and corrected. The smart error sums are based on 2D correlation analysis which is a comparably recent methodology for analyzing spectroscopic data that has found broad application. In this contribution we mathematically generalize and break down this methodology and the smart error sums to uncover the mathematic roots and simplify it to craft a general tool beyond spectroscopic modelling. This reduction also allows a simplified discussion about limits and prospects of this new method including one of its potential future uses as a sophisticated loss function in deep learning. To support its deployment, the work includes computer code to allow reproduction of the basic results.
- Published
- 2023
- Full Text
- View/download PDF
13. Paper-Based Biosensor for the Detection of Sepsis Using MMP-9 Biomarker in FIP Mice Model
- Author
-
Nuha Khalid Alekhmimi, Zeyad Raddadi, Abdulelah A. Alabdulwahed, Shimaa Eissa, Dana Cialla-May, Jürgen Popp, Khaled Al-Kattan, and Mohammed Zourob
- Subjects
sepsis ,biomarkers ,matrix metalloproteinase ,MMP-9 ,early detection ,sepsis diagnosis ,Biotechnology ,TP248.13-248.65 - Abstract
Sepsis is an immune response to a microbial invasion that causes organ injury and dysfunction due to a systemic inflammatory response. Sepsis is a serious, life-threatening condition and a widely recognized global health challenge. Given its high death rate, it is critical to diagnose sepsis and start treatment as early as possible. There is an urgent need for a sensitive and rapid screening method for detecting sepsis. In this study, we investigated the use of MMP-9 as a biomarker for sepsis. A colorimetric paper-based biosensor was used for the detection of MMP-9 utilizing peptide-magnetic nanoparticle conjugates. The method is based on the cleavage of the MMP-9-specific peptide by the protease leading to the detaching of the magnetic beads from the sensor surface and changing of color. A fecal intraperitoneal (FIP) challenge was used to induce sepsis in mice, and an MMP-9 secretion was measured by taking blood and Bronchoalveolar Lavage (BAL) fluid samples at 1 h, 2 h, 4 h, and 20 h (early sepsis) post-challenge intervals. The results of the paper-based sensor for the detection of MMP-9 levels in blood samples and BAL samples were compared with ELISA and Western Blot. We found that both blood and BAL levels of MMP-9 increased immediately and could be detected as early as 1 h in FIP mice post-challenge. Our work adds evidence to the assertion that MMP-9 is a reliable biomarker for the detection of sepsis at early stages.
- Published
- 2023
- Full Text
- View/download PDF
14. Application of Raman spectroscopy in the hospital environment
- Author
-
Aikaterini Pistiki, Markus Salbreiter, Salwa Sultan, Petra Rösch, and Jürgen Popp
- Subjects
biofilms ,host response ,pathogen identification ,Raman spectroscopy ,Applied optics. Photonics ,TA1501-1820 ,Medical technology ,R855-855.5 - Abstract
Abstract The hospital environment is a field with unique microbiological characteristics. Pathogens evolve and spread in different areas of the hospital affecting patients and staff. In addition, a constant circulation of pathogens between the hospital and the outer environment is ongoing. In this context, an extensive management is required in order to minimize the harmful effect of hospital flora on humans as well as the natural environment. Raman spectroscopy has been shown to be an effective tool for this purpose since it is applicable in a variety of biological samples ranging from the patient samples to the hospital's wastewater. It enables the detection of infection, bacterial species identification, antimicrobial resistance determination, epidemiological typing as well as infection control, mandatory for hospital management. The biggest advantages of this analytical method are the limited time and minimal resources required in its workflow. In the current review the Raman‐based analytical methods that have been developed over years in the field of microbiology are presented and their applicability in the different areas of the hospital environment is discussed.
- Published
- 2022
- Full Text
- View/download PDF
15. Raman spectroscopic cellomics for the detection of SARS‐CoV‐2‐associated neutrophil activation after TNF‐α stimulation
- Author
-
Aikaterini Pistiki, Franziska Hornung, Anja Silge, Anuradha Ramoji, Oleg Ryabchykov, Thomas W. Bocklitz, Karina Weber, Bettina Löffler, Jürgen Popp, and Stefanie Deinhardt‐Emmer
- Subjects
Medicine (General) ,R5-920 - Published
- 2022
- Full Text
- View/download PDF
16. Comparison of Different Label-Free Raman Spectroscopy Approaches for the Discrimination of Clinical MRSA and MSSA Isolates
- Author
-
Aikaterini Pistiki, Stefan Monecke, Haodong Shen, Oleg Ryabchykov, Thomas W. Bocklitz, Petra Rösch, Ralf Ehricht, and Jürgen Popp
- Subjects
MRSA ,Raman spectroscopy ,785 nm fiber probe ,532-nm single-cell analysis ,Raman microscopy ,UV resonance Raman spectroscopy ,Microbiology ,QR1-502 - Abstract
ABSTRACT Methicillin-resistant Staphylococcus aureus (MRSA) is classified as one of the priority pathogens that threaten human health. Resistance detection with conventional microbiological methods takes several days, forcing physicians to administer empirical antimicrobial treatment that is not always appropriate. A need exists for a rapid, accurate, and cost-effective method that allows targeted antimicrobial therapy in limited time. In this pilot study, we investigate the efficacy of three different label-free Raman spectroscopic approaches to differentiate methicillin-resistant and -susceptible clinical isolates of S. aureus (MSSA). Single-cell analysis using 532 nm excitation was shown to be the most suitable approach since it captures information on the overall biochemical composition of the bacteria, predicting 87.5% of the strains correctly. UV resonance Raman microspectroscopy provided a balanced accuracy of 62.5% and was not sensitive enough in discriminating MRSA from MSSA. Excitation of 785 nm directly on the petri dish provided a balanced accuracy of 87.5%. However, the difference between the strains was derived from the dominant staphyloxanthin bands in the MRSA, a cell component not associated with the presence of methicillin resistance. This is the first step toward the development of label-free Raman spectroscopy for the discrimination of MRSA and MSSA using single-cell analysis with 532 nm excitation. IMPORTANCE Label-free Raman spectra capture the high chemical complexity of bacterial cells. Many different Raman approaches have been developed using different excitation wavelength and cell analysis methods. This study highlights the major importance of selecting the most suitable Raman approach, capable of providing spectral features that can be associated with the cell mechanism under investigation. It is shown that the approach of choice for differentiating MRSA from MSSA should be single-cell analysis with 532 nm excitation since it captures the difference in the overall biochemical composition. These results should be taken into consideration in future studies aiming for the development of label-free Raman spectroscopy as a clinical analytical tool for antimicrobial resistance determination.
- Published
- 2022
- Full Text
- View/download PDF
17. In-depth characterization of self-healing polymers based on π–π interactions
- Author
-
Josefine Meurer, Julian Hniopek, Johannes Ahner, Michael Schmitt, Jürgen Popp, Stefan Zechel, Kalina Peneva, and Martin D. Hager
- Subjects
characterization of polymers ,π–π-interactions ,self-healing polymers ,supramolecular polymers ,Science ,Organic chemistry ,QD241-441 - Abstract
The self-healing behavior of two supramolecular polymers based on π–π-interactions featuring different polymer backbones is presented. For this purpose, these polymers were synthesized utilizing a polycondensation of a perylene tetracarboxylic dianhydride with polyether-based diamines and the resulting materials were investigated using various analytical techniques. Thus, the molecular structure of the polymers could be correlated with the ability for self-healing. Moreover, the mechanical behavior was studied using rheology. The activation of the supramolecular interactions results in a breaking of these noncovalent bonds, which was investigated using IR spectroscopy, leading to a sufficient increase in mobility and, finally, a healing of the mechanical damage. This scratch-healing behavior was also quantified in detail using an indenter.
- Published
- 2021
- Full Text
- View/download PDF
18. Raman Spectroscopic Differentiation of Streptococcus pneumoniae From Other Streptococci Using Laboratory Strains and Clinical Isolates
- Author
-
Marcel Dahms, Simone Eiserloh, Jürgen Rödel, Oliwia Makarewicz, Thomas Bocklitz, Jürgen Popp, and Ute Neugebauer
- Subjects
pneumococcus ,bacteria ,raman spectroscopy ,binary PLS-DA classification models ,streptococcus ,clinical isolates ,Microbiology ,QR1-502 - Abstract
Streptococcus pneumoniae, commonly referred to as pneumococci, can cause severe and invasive infections, which are major causes of communicable disease morbidity and mortality in Europe and globally. The differentiation of S. pneumoniae from other Streptococcus species, especially from other oral streptococci, has proved to be particularly difficult and tedious. In this work, we evaluate if Raman spectroscopy holds potential for a reliable differentiation of S. pneumoniae from other streptococci. Raman spectra of eight different S. pneumoniae strains and four other Streptococcus species (S. sanguinis, S. thermophilus, S. dysgalactiae, S. pyogenes) were recorded and their spectral features analyzed. Together with Raman spectra of 59 Streptococcus patient isolates, they were used to train and optimize binary classification models (PLS-DA). The effect of normalization on the model accuracy was compared, as one example for optimization potential for future modelling. Optimized models were used to identify S. pneumoniae from other streptococci in an independent, previously unknown data set of 28 patient isolates. For this small data set balanced accuracy of around 70% could be achieved. Improvement of the classification rate is expected with optimized model parameters and algorithms as well as with a larger spectral data base for training.
- Published
- 2022
- Full Text
- View/download PDF
19. Biosensing Platform for the Detection of Biomarkers for ALI/ARDS in Bronchoalveolar Lavage Fluid of LPS Mice Model
- Author
-
Nuha Khalid Alekhmimi, Dana Cialla-May, Qasem Ramadan, Shimaa Eissa, Jürgen Popp, Khaled Al-Kattan, and Mohammed Zourob
- Subjects
ALI/ARDS ,biosensors ,PR3 ,human NE ,MMP-2 ,Biotechnology ,TP248.13-248.65 - Abstract
Acute respiratory distress syndrome (ARDS) is a worldwide health concern. The pathophysiological features of ALI/ARDS include a pulmonary immunological response. The development of a rapid and low-cost biosensing platform for the detection of ARDS is urgently needed. In this study, we report the development of a paper-based multiplexed sensing platform to detect human NE, PR3 and MMP-2 proteases. Through monitoring the three proteases in infected mice after the intra-nasal administration of LPS, we showed that these proteases played an essential role in ALI/ARDS. The paper-based sensor utilized a colorimetric detection approach based on the cleavage of peptide–magnetic nanoparticle conjugates, which led to a change in the gold nanoparticle-modified paper sensor. The multiplexing of human NE, PR3 and MMP-2 proteases was tested and compared after 30 min, 2 h, 4 h and 24 h of LPS administration. The multiplexing platform of the three analytes led to relatively marked peptide cleavage occurring only after 30 min and 24 h. The results demonstrated that MMP-2, PR3 and human NE can provide a promising biosensing platform for ALI/ARDS in infected mice at different stages. MMP-2 was detected at all stages (30 min–24 h); however, the detection of human NE and PR3 can be useful for early- (30 min) and late-stage (24 h) detection of ALI/ARDS. Further studies are necessary to apply these potential diagnostic biosensing platforms to detect ARDS in patients.
- Published
- 2023
- Full Text
- View/download PDF
20. Simple, Fast and Convenient Magnetic Bead-Based Sample Preparation for Detecting Viruses via Raman-Spectroscopy
- Author
-
Susanne Pahlow, Marie Richard-Lacroix, Franziska Hornung, Nilay Köse-Vogel, Thomas G. Mayerhöfer, Julian Hniopek, Oleg Ryabchykov, Thomas Bocklitz, Karina Weber, Ralf Ehricht, Bettina Löffler, Stefanie Deinhardt-Emmer, and Jürgen Popp
- Subjects
viruses ,SARS-CoV-2 ,Raman spectroscopy ,magnetic beads ,sample preparation ,Biotechnology ,TP248.13-248.65 - Abstract
We introduce a magnetic bead-based sample preparation scheme for enabling the Raman spectroscopic differentiation of severe acute respiratory syndrome coronavirus type 2 (SARS-CoV-2)-positive and -negative samples. The beads were functionalized with the angiotensin-converting enzyme 2 (ACE2) receptor protein, which is used as a recognition element to selectively enrich SARS-CoV-2 on the surface of the magnetic beads. The subsequent Raman measurements directly enable discriminating SARS-CoV-2-positive and -negative samples. The proposed approach is also applicable for other virus species when the specific recognition element is exchanged. A series of Raman spectra were measured on three types of samples, namely SARS-CoV-2, Influenza A H1N1 virus and a negative control. For each sample type, eight independent replicates were considered. All of the spectra are dominated by the magnetic bead substrate and no obvious differences between the sample types are apparent. In order to address the subtle differences in the spectra, we calculated different correlation coefficients, namely the Pearson coefficient and the Normalized cross correlation coefficient. By comparing the correlation with the negative control, differentiating between SARS-CoV-2 and Influenza A virus is possible. This study provides a first step towards the detection and potential classification of different viruses with the use of conventional Raman spectroscopy.
- Published
- 2023
- Full Text
- View/download PDF
21. Raman Spectroscopic Imaging of Human Bladder Resectates towards Intraoperative Cancer Assessment
- Author
-
Christoph Krafft, Jürgen Popp, Peter Bronsert, and Arkadiusz Miernik
- Subjects
Raman spectroscopy ,bladder cancer ,hyperspectral unmixing ,microplastic ,pigment ,Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,RC254-282 - Abstract
Raman spectroscopy offers label-free assessment of bladder tissue for in vivo and ex vivo intraoperative applications. In a retrospective study, control and cancer specimens were prepared from ten human bladder resectates. Raman microspectroscopic images were collected from whole tissue samples in a closed chamber at 785 nm laser excitation using a 20× objective lens and 250 µm step size. Without further preprocessing, Raman images were decomposed by the hyperspectral unmixing algorithm vertex component analysis into endmember spectra and their abundancies. Hierarchical cluster analysis distinguished endmember Raman spectra that were assigned to normal bladder, bladder cancer, necrosis, epithelium and lipid inclusions. Interestingly, Raman spectra of microplastic particles, pigments or carotenoids were detected in 13 out of 20 specimens inside tissue and near tissue margins and their identity was confirmed by spectral library surveys. Hypotheses about the origin of these foreign materials are discussed. In conclusion, our Raman workflow and data processing protocol with minimal user interference offers advantages for future clinical translation such as intraoperative tumor detection and label-free material identification in complex matrices.
- Published
- 2023
- Full Text
- View/download PDF
22. Deep learning-based classification of blue light cystoscopy imaging during transurethral resection of bladder tumors
- Author
-
Nairveen Ali, Christian Bolenz, Tilman Todenhöfer, Arnulf Stenzel, Peer Deetmar, Martin Kriegmair, Thomas Knoll, Stefan Porubsky, Arndt Hartmann, Jürgen Popp, Maximilian C. Kriegmair, and Thomas Bocklitz
- Subjects
Medicine ,Science - Abstract
Abstract Bladder cancer is one of the top 10 frequently occurring cancers and leads to most cancer deaths worldwide. Recently, blue light (BL) cystoscopy-based photodynamic diagnosis was introduced as a unique technology to enhance the detection of bladder cancer, particularly for the detection of flat and small lesions. Here, we aim to demonstrate a BL image-based artificial intelligence (AI) diagnostic platform using 216 BL images, that were acquired in four different urological departments and pathologically identified with respect to cancer malignancy, invasiveness, and grading. Thereafter, four pre-trained convolution neural networks were utilized to predict image malignancy, invasiveness, and grading. The results indicated that the classification sensitivity and specificity of malignant lesions are 95.77% and 87.84%, while the mean sensitivity and mean specificity of tumor invasiveness are 88% and 96.56%, respectively. This small multicenter clinical study clearly shows the potential of AI based classification of BL images allowing for better treatment decisions and potentially higher detection rates.
- Published
- 2021
- Full Text
- View/download PDF
23. Morpho-molecular signal correlation between optical coherence tomography and Raman spectroscopy for superior image interpretation and clinical diagnosis
- Author
-
Iwan W. Schie, Fabian Placzek, Florian Knorr, Eliana Cordero, Lara M. Wurster, Gregers G. Hermann, Karin Mogensen, Thomas Hasselager, Wolfgang Drexler, Jürgen Popp, and Rainer A. Leitgeb
- Subjects
Medicine ,Science - Abstract
Abstract The combination of manifold optical imaging modalities resulting in multimodal optical systems allows to discover a larger number of biomarkers than using a single modality. The goal of multimodal imaging systems is to increase the diagnostic performance through the combination of complementary modalities, e.g. optical coherence tomography (OCT) and Raman spectroscopy (RS). The physical signal origins of OCT and RS are distinctly different, i.e. in OCT it is elastic back scattering of photons, due to a change in refractive index, while in RS it is the inelastic scattering between photons and molecules. Despite those diverse characteristics both modalities are also linked via scattering properties and molecular composition of tissue. Here, we investigate for the first time the relation of co-registered OCT and RS signals of human bladder tissue, to demonstrate that the signals of these complementary modalities are inherently intertwined, enabling a direct but more importantly improved interpretation and better understanding of the other modality. This work demonstrates that the benefit for using two complementary imaging approaches is, not only the increased diagnostic value, but the increased information and better understanding of the signal origins of both modalities. This evaluation confirms the advantages for using multimodal imaging systems and also paves the way for significant further improved understanding and clinically interpretation of both modalities in the future.
- Published
- 2021
- Full Text
- View/download PDF
24. Rapid Colorimetric Detection of Pseudomonas aeruginosa in Clinical Isolates Using a Magnetic Nanoparticle Biosensor
- Author
-
Sahar Alhogail, Ghadeer A.R.Y. Suaifan, Floris J. Bikker, Wendy E. Kaman, Karina Weber, Dana Cialla-May, Jürgen Popp, and Mohammed M. Zourob
- Subjects
Chemistry ,QD1-999 - Published
- 2019
- Full Text
- View/download PDF
25. New Methods for the Functionalization of Polymer Matrices with Thiomolybdate Clusters Applied for Hydrogen Evolution Reaction Catalysis
- Author
-
Nadine Hannewald, Julian Hniopek, Magdalena Heiland, Stefan Zechel, Michael Schmitt, Carsten Streb, Jürgen Popp, Martin D. Hager, and Ulrich S. Schubert
- Subjects
hydrogen evolution reaction ,hydrogen generation ,polymer catalysts ,Environmental technology. Sanitary engineering ,TD1-1066 ,Renewable energy sources ,TJ807-830 - Abstract
The embedding of molecular reactive species such as catalysts into polymers opens up new avenues for advanced composite design for energy technologies and beyond. Herein, two new approaches for the connection of molecular molybdenum sulfide clusters and synthetic polymers are presented. The approach one is based on the utilization of a molecular molybdenum sulfide cluster as a potential chain transfer agent in a free radical polymerization process. The second one is a postpolymerization functionalization of a thiol end‐functionalized polymer with a thiomolybdate cluster. Detailed characterization using Raman spectroscopy reveals the successful functionalization in the latter approach, whereas in the other one, a noncovalent interaction is suggested. Furthermore, visible light‐driven hydrogen evolution demonstrates that the molecular molybdenum sulfide cluster retains its catalytic activity after the combination with polymers.
- Published
- 2021
- Full Text
- View/download PDF
26. Leukocyte Activation Profile Assessed by Raman Spectroscopy Helps Diagnosing Infection and Sepsis
- Author
-
Anuradha Ramoji, PhD, Daniel Thomas-Rüddel, MD, Oleg Ryabchykov, PhD, Michael Bauer, MD, Natalie Arend, MSc, Evangelos J. Giamarellos-Bourboulis, MD, Jesper Eugen-Olsen, PhD, Michael Kiehntopf, MD, Thomas Bocklitz, PhD, Jürgen Popp, PhD, Frank Bloos, MD, PhD, and Ute Neugebauer, PhD
- Subjects
Medical emergencies. Critical care. Intensive care. First aid ,RC86-88.9 - Abstract
Objectives:. Leukocytes are first responders to infection. Their activation state can reveal information about specific host immune response and identify dysregulation in sepsis. This study aims to use the Raman spectroscopic fingerprints of blood-derived leukocytes to differentiate inflammation, infection, and sepsis in hospitalized patients. Diagnostic sensitivity and specificity shall demonstrate the added value of the direct characterization of leukocyte’s phenotype. Design:. Prospective nonrandomized, single-center, observational phase-II study (DRKS00006265). Setting:. Jena University Hospital, Germany. Patients:. Sixty-one hospitalized patients (19 with sterile inflammation, 23 with infection without organ dysfunction, 18 with sepsis according to Sepsis-3 definition). Interventions:. None (blood withdrawal). Measurements AND MAIN RESULTS:. Individual peripheral blood leukocytes were characterized by Raman spectroscopy. Reference diagnostics included established clinical scores, blood count, and biomarkers (C-reactive protein, procalcitonin and interleukin-6). Binary classification models using Raman data were able to distinguish patients with infection from patients without infection, as well as sepsis patients from patients without sepsis, with accuracies achieved with established biomarkers. Compared with biomarker information alone, an increase of 10% (to 93%) accuracy for the detection of infection and an increase of 18% (to 92%) for detection of sepsis were reached by adding the Raman information. Leukocytes from sepsis patients showed different Raman spectral features in comparison to the patients with infection that point to the special immune phenotype of sepsis patients. Conclusions:. Raman spectroscopy can extract information on leukocyte’s activation state in a nondestructive, label-free manner to differentiate sterile inflammation, infection, and sepsis.
- Published
- 2021
- Full Text
- View/download PDF
27. corr2D: Implementation of Two-Dimensional Correlation Analysis in R
- Author
-
Robert Geitner, Robby Fritzsch, Jürgen Popp, and Thomas W. Bocklitz
- Subjects
correlation analysis ,2d correlation ,spectroscopy ,r package ,corr2d ,Statistics ,HA1-4737 - Abstract
In the package corr2D two-dimensional correlation analysis is implemented in R. This paper describes how two-dimensional correlation analysis is done in the package and how the mathematical equations are translated into R code. The paper features a simple tutorial with executable code for beginners, insight into the calculations done before the correlation analysis, a detailed look at the parallelization of the fast Fourier transformation based correlation analysis and a speed test of the calculation. The package corr2D offers the possibility to preprocess, correlate and postprocess spectroscopic data using exclusively the R language. Thus, corr2D is a welcome addition to the toolbox of spectroscopists and makes two-dimensional correlation analysis more accessible and transparent.
- Published
- 2019
- Full Text
- View/download PDF
28. Rapid Isolation and Identification of Pneumonia-Associated Pathogens from Sputum Samples Combining an Innovative Sample Preparation Strategy and Array-Based Detection
- Author
-
Susanne Pahlow, Lydia Lehniger, Stefanie Hentschel, Barbara Seise, Sascha D. Braun, Ralf Ehricht, Albrecht Berg, Jürgen Popp, and Karina Weber
- Subjects
Chemistry ,QD1-999 - Published
- 2019
- Full Text
- View/download PDF
29. Revealing the Chemical Composition of Birch Pollen Grains by Raman Spectroscopic Imaging
- Author
-
Clara Stiebing, Nele Post, Claudia Schindler, Bianca Göhrig, Harald Lux, Jürgen Popp, Astrid Heutelbeck, and Iwan W. Schie
- Subjects
vibrational spectroscopy ,Betula ,allergy ,sporopollenin ,multiple curve resolution ,Biology (General) ,QH301-705.5 ,Chemistry ,QD1-999 - Abstract
The investigation of the biochemical composition of pollen grains is of the utmost interest for several environmental aspects, such as their allergenic potential and their changes in growth conditions due to climatic factors. In order to fully understand the composition of pollen grains, not only is an in-depth analysis of their molecular components necessary but also spatial information of, e.g., the thickness of the outer shell, should be recorded. However, there is a lack of studies using molecular imaging methods for a spatially resolved biochemical composition on a single-grain level. In this study, Raman spectroscopy was implemented as an analytical tool to investigate birch pollen by imaging single pollen grains and analyzing their spectral profiles. The imaging modality allowed us to reveal the layered structure of pollen grains based on the biochemical information of the recorded Raman spectra. Seven different birch pollen species collected at two different locations in Germany were investigated and compared. Using chemometric algorithms such as hierarchical cluster analysis and multiple-curve resolution, several components of the grain wall, such as sporopollenin, as well as the inner core presenting high starch concentrations, were identified and quantified. Differences in the concentrations of, e.g., sporopollenin, lipids and proteins in the pollen species at the two different collection sites were found, and are discussed in connection with germination and other growth processes.
- Published
- 2022
- Full Text
- View/download PDF
30. Synthesis and Characterization of Metallopolymer Networks Featuring Triple Shape-Memory Ability Based on Different Reversible Metal Complexes
- Author
-
Josefine Meurer, Thomas Bätz, Julian Hniopek, Milena Jäger, Stefan Zechel, Michael Schmitt, Jürgen Popp, Martin D. Hager, and Ulrich S. Schubert
- Subjects
smart materials ,responsive polymers ,triple shape-memory ,metallopolymers ,metal ligand interaction ,Organic chemistry ,QD241-441 - Abstract
This study presents the synthesis and characterization of metallopolymer networks with a triple shape-memory ability. A covalently crosslinked polymer network featuring two different additional ligands in its side chains is synthesized via free radical polymerization (FRP). The subsequent addition of different metal salts leads to the selective formation of complexes with two different association constants (Ka), proven via isothermal titration calorimetry (ITC). Those two supramolecular crosslinks feature different activation temperatures and can act as two individual switching units enabling the fixation and recovery of two temporary shapes. The presented samples were investigated in a detailed fashion via differential scanning calorimetry (DSC), thermogravimetric analysis (TGA), and FT-Raman spectroscopy. Furthermore, thermo-mechanical analyses (TMA) revealed excellent dual and triple shape-memory abilities of the presented metallopolymer networks.
- Published
- 2022
- Full Text
- View/download PDF
31. Three step flow focusing enables image-based discrimination and sorting of late stage 1 Haematococcus pluvialis cells.
- Author
-
Daniel Kraus, Andreas Kleiber, Enrico Ehrhardt, Matthias Leifheit, Peter Horbert, Matthias Urban, Nils Gleichmann, Günter Mayer, Jürgen Popp, and Thomas Henkel
- Subjects
Medicine ,Science - Abstract
Label-free and gentle separation of cell stages with desired target properties from mixed stage populations are a major research task in modern biotechnological cultivation process and optimization of micro algae. The reported microfluidic sorter system (MSS) allows the subsequent investigation of separated subpopulations. The implementation of a viability preserving MSS is shown for separation of late stage 1 Haematococcus pluvialis (HP) cells form a mixed stage population. The MSS combines a three-step flow focusing unit for aligning the cells in single file transportation mode at the center of the microfluidic channel with a pure hydrodynamic sorter structure for cell sorting. Lateral displacement of the cells into one of the two outlet channels is generated by piezo-actuated pump chambers. In-line decision making for sorting is based on a user-definable set of image features and properties. The reported MSS significantly increased the purity of target cells in the sorted population (94%) in comparison to the initial mixed stage population (19%).
- Published
- 2021
- Full Text
- View/download PDF
32. Aptamers: Potential Diagnostic and Therapeutic Agents for Blood Diseases
- Author
-
Maher M. Aljohani, Dana Cialla-May, Jürgen Popp, Raja Chinnappan, Khaled Al-Kattan, and Mohammed Zourob
- Subjects
aptamers ,diagnostic ,therapeutic ,blood diseases ,Organic chemistry ,QD241-441 - Abstract
Aptamers are RNA/DNA oligonucleotide molecules that specifically bind to a targeted complementary molecule. As potential recognition elements with promising diagnostic and therapeutic applications, aptamers, such as monoclonal antibodies, could provide many treatment and diagnostic options for blood diseases. Aptamers present several superior features over antibodies, including a simple in vitro selection and production, ease of modification and conjugation, high stability, and low immunogenicity. Emerging as promising alternatives to antibodies, aptamers could overcome the present limitations of monoclonal antibody therapy to provide novel diagnostic, therapeutic, and preventive treatments for blood diseases. Researchers in several biomedical areas, such as biomarker detection, diagnosis, imaging, and targeted therapy, have widely investigated aptamers, and several aptamers have been developed over the past two decades. One of these is the pegaptanib sodium injection, an aptamer-based therapeutic that functions as an anti-angiogenic medicine, and it is the first aptamer approved by the U.S. Food and Drug Administration (FDA) for therapeutic use. Several other aptamers are now in clinical trials. In this review, we highlight the current state of aptamers in the clinical trial program and introduce some promising aptamers currently in pre-clinical development for blood diseases.
- Published
- 2022
- Full Text
- View/download PDF
33. Investigating Origins of FLIm Contrast in Atherosclerotic Lesions Using Combined FLIm-Raman Spectroscopy
- Author
-
Julien Bec, Tanveer Ahmed Shaik, Christoph Krafft, Thomas W. Bocklitz, Alba Alfonso-Garcia, Kenneth B. Margulies, Jürgen Popp, and Laura Marcu
- Subjects
imaging ,spectroscopy ,atherosclerosis ,Raman spectroscopy ,FLIm ,time resolved fluorescence ,Diseases of the circulatory (Cardiovascular) system ,RC666-701 - Abstract
Background: Fluorescence lifetime imaging (FLIm) is a spectroscopic imaging technique able to characterize the composition of luminal surface of arterial vessels. Studies of human coronary samples demonstrated that distinct atherosclerotic lesion types are characterized by FLIm features associate with distinct tissue molecular makeup. While conventional histology has provided indications about potential sources of molecular contrast, specific information about the origin of FLIm signals is lacking. Here we investigate whether Raman spectroscopy, a technique able to evaluate chemical content of biological samples, can provide additional insight into the origin of FLIm contrast.Methods: Six human coronary artery samples were imaged using FLIm (355 nm excitation)-Raman spectroscopy (785 nm excitation) via a multimodal fiber optic probe. The spatial distribution of molecular contrast in FLIm images was analyzed in relationship with histological findings. Raman data was investigated using an endmember technique and compared with histological findings. A descriptive modeling approach based on multivariate regression was used to identify Raman bands related with changes in lifetime in four spectral channels (violet: 387/35 nm, blue: 443/29 nm, green: 546/38 nm, and red: 628/53 nm).Results: Fluorescence lifetime variations in the violet, blue and green spectral bands were observed for distinct areas of each tissue sample associated with distinct pathologies. Analysis of Raman signals from areas associated with normal, pathological intimal thickening, and fibrocalcific regions demonstrated the presence of hydroxyapatite, collagenous proteins, carotene, cholesterol, and triglycerides. The FLIm and Raman descriptive modeling analysis indicated that lifetime increase in the violet spectral band was associated with increased presence of cholesterol and carotenes, a new finding consistent with LDL accumulation in atherosclerotic lesions, and not with collagen proteins, as expected from earlier studies.Conclusions: The systematic, quantitative analysis of the multimodal FLIm-Raman dataset using a descriptive modeling approach led to the identification of LDL accumulation as the primary source of lifetime contrast in atherosclerotic lesions in the violet spectral range. Earlier FLIm validation studies relying on histopathological findings had associated this contrast to increased collagen content, also present in advanced lesions, thus demonstrating the benefits of alternative validation methods.
- Published
- 2020
- Full Text
- View/download PDF
34. Response of the wood-decay fungus Schizophyllum commune to co-occurring microorganisms.
- Author
-
Katrin Krause, Elke-Martina Jung, Julia Lindner, Imam Hardiman, Jessica Poetschner, Soumya Madhavan, Christian Matthäus, Marco Kai, Riya Christina Menezes, Jürgen Popp, Aleš Svatoš, and Erika Kothe
- Subjects
Medicine ,Science - Abstract
Microorganisms are constantly interacting in a given environment by a constant exchange of signaling molecules. In timber, wood-decay fungi will come into contact with other fungi and bacteria. In naturally bleached wood, dark, pigmented lines arising from confrontation of two fungi often hint at such interactions. The metabolites (and pigment) exchange was investigated using the lignicolous basidiomycete Schizophyllum commune, and co-occurring fungi and bacteria inoculated directly on sterilized wood, or on media. In interactions with competitive wood degrading fungi, yeasts or bacteria, different competition strategies and communication types were observed, and stress reactions, as well as competitor-induced enzymes or pigments were analyzed. Melanin, indole, flavonoids and carotenoids were shown to be induced in S. commune interactions. The induced genes included multi-copper oxidases lcc1, lcc2, mco1, mco2, mco3 and mco4, possibly involved in both pigment production and lignin degradation typical for wood bleaching by wood-decay fungi.
- Published
- 2020
- Full Text
- View/download PDF
35. A Model System for Sensitive Detection of Viable E. coli Bacteria Combining Direct Viability PCR and a Novel Microarray-Based Detection Approach
- Author
-
Lydia Lehniger, Anne Rudloff, Sibyll Pollok, Norman Große, Kristin Wessel, Monique Brendel, Jürgen Popp, and Karina Weber
- Subjects
E. coli ,water monitoring ,direct viability nested PCR ,microarray-based DNA hybridization ,Biochemistry ,QD415-436 - Abstract
We established an innovative approach that included direct, viability, and nested PCR for rapid and reliable identification of the fecal indicator organism Escherichia coli (E. coli). Direct PCR enabled successful amplification of the target uidA gene, omitting a prior DNA isolation or purification step. Furthermore, we applied viability PCR (v-PCR) to ensure the detection of only relevant viable bacterial cells. The principle involves the binding of propidium monoazide (PMA), a selective nucleic acid intercalating dye, to accessible DNA of heat killed bacteria cells and, consequently, allows viable and heat killed E. coli cells to be discriminated. To ensure high sensitivity, direct v-PCR was followed by a nested PCR step. The resulting amplicons were analyzed by a rapid 30 min microarray-based DNA hybridization assay for species-specific DNA detection of E. coli. A positive signal was indicated by enzymatically generated silver nanoparticle deposits, which served as robust endpoint signals allowing an immediate visual readout. The presented novel protocol allows the detection of 1 × 101 viable E. coli cells per PCR run.
- Published
- 2021
- Full Text
- View/download PDF
36. Biochemical Analysis of Leukocytes after In Vitro and In Vivo Activation with Bacterial and Fungal Pathogens Using Raman Spectroscopy
- Author
-
Aikaterini Pistiki, Anuradha Ramoji, Oleg Ryabchykov, Daniel Thomas-Rüddel, Adrian T. Press, Oliwia Makarewicz, Evangelos J. Giamarellos-Bourboulis, Michael Bauer, Thomas Bocklitz, Jürgen Popp, and Ute Neugebauer
- Subjects
Raman microspectroscopy ,leukocytes ,infection model ,Staphylococcus aureus ,Klebsiella pneumoniae ,Candida albicans ,Biology (General) ,QH301-705.5 ,Chemistry ,QD1-999 - Abstract
Biochemical information from activated leukocytes provide valuable diagnostic information. In this study, Raman spectroscopy was applied as a label-free analytical technique to characterize the activation pattern of leukocyte subpopulations in an in vitro infection model. Neutrophils, monocytes, and lymphocytes were isolated from healthy volunteers and stimulated with heat-inactivated clinical isolates of Candida albicans, Staphylococcus aureus, and Klebsiella pneumoniae. Binary classification models could identify the presence of infection for monocytes and lymphocytes, classify the type of infection as bacterial or fungal for neutrophils, monocytes, and lymphocytes and distinguish the cause of infection as Gram-negative or Gram-positive bacteria in the monocyte subpopulation. Changes in single-cell Raman spectra, upon leukocyte stimulation, can be explained with biochemical changes due to the leukocyte’s specific reaction to each type of pathogen. Raman spectra of leukocytes from the in vitro infection model were compared with spectra from leukocytes of patients with infection (DRKS-ID: DRKS00006265) with the same pathogen groups, and a good agreement was revealed. Our study elucidates the potential of Raman spectroscopy-based single-cell analysis for the differentiation of circulating leukocyte subtypes and identification of the infection by probing the molecular phenotype of those cells.
- Published
- 2021
- Full Text
- View/download PDF
37. Raman Stable Isotope Probing of Bacteria in Visible and Deep UV-Ranges
- Author
-
Georgette Azemtsop Matanfack, Aikaterini Pistiki, Petra Rösch, and Jürgen Popp
- Subjects
Raman microspectroscopy ,Raman UV-resonance ,bacterial cells ,DNA labeling ,metabolic activity ,Science - Abstract
Raman stable isotope probing (Raman-SIP) is an excellent technique that can be used to access the overall metabolism of microorganisms. Recent studies have mainly used an excitation wavelength in the visible range to characterize isotopically labeled bacteria. In this work, we used UV resonance Raman spectroscopy (UVRR) to evaluate the spectral red-shifts caused by the uptake of isotopes (13C, 15N, 2H(D) and 18O) in E. coli cells. Moreover, we present a new approach based on the extraction of labeled DNA in combination with UVRR to identify metabolically active cells. The proof-of-principle study on E. coli revealed heterogeneities in the Raman features of both the bacterial cells and the extracted DNA after labeling with 13C, 15N, and D. The wavelength of choice for studying 18O- and deuterium-labeled cells is 532 nm is, while 13C-labeled cells can be investigated with visible and deep UV wavelengths. However, 15N-labeled cells are best studied at the excitation wavelength of 244 nm since nucleic acids are in resonance at this wavelength. These results highlight the potential of the presented approach to identify active bacterial cells. This work can serve as a basis for the development of new techniques for the rapid and efficient detection of active bacteria cells without the need for a cultivation step.
- Published
- 2021
- Full Text
- View/download PDF
38. Noise Sources and Requirements for Confocal Raman Spectrometers in Biosensor Applications
- Author
-
Izabella J. Jahn, Alexej Grjasnow, Henry John, Karina Weber, Jürgen Popp, and Walter Hauswald
- Subjects
confocal Raman spectrometer ,biosensor ,signal-to-noise ,bacteria ,fluorescence background ,Chemical technology ,TP1-1185 - Abstract
Raman spectroscopy probes the biochemical composition of samples in a non-destructive, non-invasive and label-free fashion yielding specific information on a molecular level. Nevertheless, the Raman effect is very weak. The detection of all inelastically scattered photons with highest efficiency is therefore crucial as well as the identification of all noise sources present in the system. Here we provide a study for performance comparison and assessment of different spectrometers for confocal Raman spectroscopy in biosensor applications. A low-cost, home-built Raman spectrometer with a complementary metal-oxide-semiconductor (CMOS) camera, a middle price-class mini charge-coupled device (CCD) Raman spectrometer and a laboratory grade confocal Raman system with a deeply cooled CCD detector are compared. It is often overlooked that the sample itself is the most important “optical” component in a Raman spectrometer and its properties contribute most significantly to the signal-to-noise ratio. For this purpose, different representative samples: a crystalline silicon wafer, a polypropylene sample and E. coli bacteria were measured under similar conditions using the three confocal Raman spectrometers. We show that biosensor applications do not in every case profit from the most expensive equipment. Finally, a small Raman database of three different bacteria species is set up with the middle price-class mini CCD Raman spectrometer in order to demonstrate the potential of a compact setup for pathogen discrimination.
- Published
- 2021
- Full Text
- View/download PDF
39. Surface-Enhanced Raman Spectroscopy to Characterize Different Fractions of Extracellular Vesicles from Control and Prostate Cancer Patients
- Author
-
Eric Boateng Osei, Liliia Paniushkina, Konrad Wilhelm, Jürgen Popp, Irina Nazarenko, and Christoph Krafft
- Subjects
extracellular vesicles ,Raman spectroscopy ,surface enhanced Raman spectroscopy ,Biology (General) ,QH301-705.5 - Abstract
Extracellular vesicles (EVs) are membrane-enclosed structures ranging in size from about 60 to 800 nm that are released by the cells into the extracellular space; they have attracted interest as easily available biomarkers for cancer diagnostics. In this study, EVs from plasma of control and prostate cancer patients were fractionated by differential centrifugation at 5000× g, 12,000× g and 120,000× g. The remaining supernatants were purified by ultrafiltration to produce EV-depleted free-circulating (fc) fractions. Spontaneous Raman and surface-enhanced Raman spectroscopy (SERS) at 785 nm excitation using silver nanoparticles (AgNPs) were employed as label-free techniques to collect fingerprint spectra and identify the fractions that best discriminate between control and cancer patients. SERS spectra from 10 µL droplets showed an enhanced Raman signature of EV-enriched fractions that were much more intense for cancer patients than controls. The Raman spectra of dehydrated pellets of EV-enriched fractions without AgNPs were dominated by spectral contributions of proteins and showed variations in S-S stretch, tryptophan and protein secondary structure bands between control and cancer fractions. We conclude that the AgNPs-mediated SERS effect strongly enhances Raman bands in EV-enriched fractions, and the fractions, EV12 and EV120 provide the best separation of cancer and control patients by Raman and SERS spectra.
- Published
- 2021
- Full Text
- View/download PDF
40. Raman imaging of macrophages incubated with triglyceride-enriched oxLDL visualizes translocation of lipids between endocytic vesicles and lipid droplets
- Author
-
Clara Stiebing, Lisa Schmölz, Maria Wallert, Christian Matthäus, Stefan Lorkowski, and Jürgen Popp
- Subjects
Raman spectroscopy ,β-carotene ,tripalmitate ,endocytosis ,atherosclerosis ,oxidized low density lipoprotein ,Biochemistry ,QD415-436 - Abstract
Raman spectroscopic imaging was used to investigate the uptake of oxidized LDLs (oxLDLs) by human macrophages. To better understand the endocytic pathway and the intracellular fate of modified lipoproteins is of foremost interest with regard to the development of atherosclerotic plaques. To obtain information on the storage process of lipids caused by oxLDL uptake, Raman spectroscopic imaging was used because of its unique chemical specificity, especially for lipids. For the present study, a protocol was established to incorporate deuterated tripalmitate into oxLDL. Subsequently, human THP-1 macrophages were incubated for different time points and their chemical composition was analyzed using Raman spectroscopic imaging. β-Carotene was found to be a reliable marker molecule for the uptake of lipoproteins into macrophages. In addition, lipoprotein administration led to small endocytic vesicles with different concentrations of deuterated lipids within the cells. For the first time, the translocation of deuterated lipids from endocytic vesicles into lipid droplets over time is reported in mature human THP-1 macrophages.
- Published
- 2017
- Full Text
- View/download PDF
41. Thermal illumination limits in 3D Raman microscopy: A comparison of different sample illumination strategies to obtain maximum imaging speed.
- Author
-
Walter Hauswald, Ronny Förster, Jürgen Popp, and Rainer Heintzmann
- Subjects
Medicine ,Science - Abstract
Confocal Raman microscopy is a powerful tool for material science and biomedical research. However, the low Raman scattering cross-section limits the working speed, which reduces the applicability for large and sensitive samples. Here, we discuss the fundamental physical limits of Raman spectroscopy with respect to signal-to-noise, sample load and how to achieve maximal imaging speed. For this, we develop a simple model to describe arbitrary far field light microscopes and their thermal influence on the sample. This model is used to compare the practical applicability of point- and line-confocal microscopes as well as wide-field-, light sheet- and light line illumination, for the measurement of 3D biological samples. The parallelization degree of the illumination can positively affect the imaging speed as long as it is not limited by thermal sample heating. In case of heat build-up inside the sample, the advantages of parallelization can be lost due to the required attenuation of excitation and the working speed can drop below that of a sequential method. We show that for point like illumination, the exposure time is thermally not as critical for the sample as the irradiance, while for volume like illumination, the exposure time and irradiance result in the same thermal effect. The results of our theoretical study are experimentally confirmed and suggest new concepts of Raman microscopy, thus extending its applicability. The developed model can be applied to Raman imaging as well as to other modes (e.g. two- or three- photon imaging, STED, PALM/STORM, MINFLUX) where thermal effects impose a practical limit due to the high irradiance required.
- Published
- 2019
- Full Text
- View/download PDF
42. Author Correction: Systematic evaluation of particle loss during handling in the percutaneous transluminal angioplasty for eight different drug-coated balloons
- Author
-
Andreas Heinrich, Martin S. Engler, Felix V. Güttler, Christian Matthäus, Jürgen Popp, and Ulf K.-M. Teichgräber
- Subjects
Medicine ,Science - Abstract
An amendment to this paper has been published and can be accessed via a link at the top of the paper.
- Published
- 2021
- Full Text
- View/download PDF
43. Vibrational Spectroscopic Investigation of Blood Plasma and Serum by Drop Coating Deposition for Clinical Application
- Author
-
Jing Huang, Nairveen Ali, Elsie Quansah, Shuxia Guo, Michel Noutsias, Tobias Meyer-Zedler, Thomas Bocklitz, Jürgen Popp, Ute Neugebauer, and Anuradha Ramoji
- Subjects
plasma ,serum ,coffee-ring effect ,cardiac patients ,vibrational spectroscopy ,fluorescence lifetime ,Biology (General) ,QH301-705.5 ,Chemistry ,QD1-999 - Abstract
In recent decades, vibrational spectroscopic methods such as Raman and FT-IR spectroscopy are widely applied to investigate plasma and serum samples. These methods are combined with drop coating deposition techniques to pre-concentrate the biomolecules in the dried droplet to improve the detected vibrational signal. However, most often encountered challenge is the inhomogeneous redistribution of biomolecules due to the coffee-ring effect. In this study, the variation in biomolecule distribution within the dried-sample droplet has been investigated using Raman and FT-IR spectroscopy and fluorescence lifetime imaging method. The plasma-sample from healthy donors were investigated to show the spectral differences between the inner and outer-ring region of the dried-sample droplet. Further, the preferred location of deposition of the most abundant protein albumin in the blood during the drying process of the plasma has been illustrated by using deuterated albumin. Subsequently, two patients with different cardiac-related diseases were investigated exemplarily to illustrate the variation in the pattern of plasma and serum biomolecule distribution during the drying process and its impact on patient-stratification. The study shows that a uniform sampling position of the droplet, both at the inner and the outer ring, is necessary for thorough clinical characterization of the patient’s plasma and serum sample using vibrational spectroscopy.
- Published
- 2021
- Full Text
- View/download PDF
44. Wide Field Spectral Imaging with Shifted Excitation Raman Difference Spectroscopy Using the Nod and Shuffle Technique
- Author
-
Florian Korinth, Elmar Schmälzlin, Clara Stiebing, Tanya Urrutia, Genoveva Micheva, Christer Sandin, André Müller, Martin Maiwald, Bernd Sumpf, Christoph Krafft, Günther Tränkle, Martin M. Roth, and Jürgen Popp
- Subjects
Raman spectroscopy ,SERDS ,wide field imaging ,nod and shuffle ,photobleaching ,autofluorescence ,Chemical technology ,TP1-1185 - Abstract
Wide field Raman imaging using the integral field spectroscopy approach was used as a fast, one shot imaging method for the simultaneous collection of all spectra composing a Raman image. For the suppression of autofluorescence and background signals such as room light, shifted excitation Raman difference spectroscopy (SERDS) was applied to remove background artifacts in Raman spectra. To reduce acquisition times in wide field SERDS imaging, we adapted the nod and shuffle technique from astrophysics and implemented it into a wide field SERDS imaging setup. In our adapted version, the nod corresponds to the change in excitation wavelength, whereas the shuffle corresponds to the shifting of charges up and down on a Charge-Coupled Device (CCD) chip synchronous to the change in excitation wavelength. We coupled this improved wide field SERDS imaging setup to diode lasers with 784.4/785.5 and 457.7/458.9 nm excitation and applied it to samples such as paracetamol and aspirin tablets, polystyrene and polymethyl methacrylate beads, as well as pork meat using multiple accumulations with acquisition times in the range of 50 to 200 ms. The results tackle two main challenges of SERDS imaging: gradual photobleaching changes the autofluorescence background, and multiple readouts of CCD detector prolong the acquisition time.
- Published
- 2020
- Full Text
- View/download PDF
45. Raman Signal Enhancement Tunable by Gold-Covered Porous Silicon Films with Different Morphology
- Author
-
Svetlana N. Agafilushkina, Olga Žukovskaja, Sergey A. Dyakov, Karina Weber, Vladimir Sivakov, Jürgen Popp, Dana Cialla-May, and Liubov A. Osminkina
- Subjects
porous silicon ,gold ,surface-enhanced Raman scattering ,plasmonics ,sensorics ,Chemical technology ,TP1-1185 - Abstract
The ease of fabrication, large surface area, tunable pore size and morphology as well surface modification capabilities of a porous silicon (PSi) layer make it widely used for sensoric applications. The pore size of a PSi layer can be an important parameter when used as a matrix for creating surface-enhanced Raman scattering (SERS) surfaces. Here, we evaluated the SERS activity of PSi with pores ranging in size from meso to macro, the surface of which was coated with gold nanoparticles (Au NPs). We found that different pore diameters in the PSi layers provide different morphology of the gold coating, from an almost monolayer to 50 nm distance between nanoparticles. Methylene blue (MB) and 4-mercaptopyridine (4-MPy) were used to describe the SERS activity of obtained Au/PSi surfaces. The best Raman signal enhancement was shown when the internal diameter of torus-shaped Au NPs is around 35 nm. To understand the role of plasmonic resonances in the observed SERS spectrum, we performed electromagnetic simulations of Raman scattering intensity as a function of the internal diameter. The results of these simulations are consistent with the obtained experimental data.
- Published
- 2020
- Full Text
- View/download PDF
46. Eosinophils and Neutrophils—Molecular Differences Revealed by Spontaneous Raman, CARS and Fluorescence Microscopy
- Author
-
Aleksandra Dorosz, Marek Grosicki, Jakub Dybas, Ewelina Matuszyk, Marko Rodewald, Tobias Meyer, Jürgen Popp, Kamilla Malek, and Malgorzata Baranska
- Subjects
eosinophils ,neutrophils ,eosinophil peroxidase ,myeloperoxidase ,lipid bodies ,Raman microscopy ,Cytology ,QH573-671 - Abstract
Leukocytes are a part of the immune system that plays an important role in the host’s defense against viral, bacterial, and fungal infections. Among the human leukocytes, two granulocytes, neutrophils (Ne) and eosinophils (EOS) play an important role in the innate immune system. For that purpose, eosinophils and neutrophils contain specific granules containing protoporphyrin-type proteins such as eosinophil peroxidase (EPO) and myeloperoxidase (MPO), respectively, which contribute directly to their anti-infection activity. Since both proteins are structurally and functionally different, they could potentially be a marker of both cells’ types. To prove this hypothesis, UV−Vis absorption spectroscopy and Raman imaging were applied to analyze EPO and MPO and their content in leukocytes isolated from the whole blood. Moreover, leukocytes can contain lipidic structures, called lipid bodies (LBs), which are linked to the regulation of immune responses and are considered to be a marker of cell inflammation. In this work, we showed how to determine the number of LBs in two types of granulocytes, EOS and Ne, using fluorescence and coherent anti-Stokes Raman scattering (CARS) microscopy. Spectroscopic differences of EPO and MPO can be used to identify these cells in blood samples, while the detection of LBs can indicate the cell inflammation process.
- Published
- 2020
- Full Text
- View/download PDF
47. FLIm and Raman Spectroscopy for Investigating Biochemical Changes of Bovine Pericardium upon Genipin Cross-Linking
- Author
-
Tanveer Ahmed Shaik, Alba Alfonso-Garcia, Martin Richter, Florian Korinth, Christoph Krafft, Laura Marcu, and Jürgen Popp
- Subjects
genipin ,cross-linking ,FLIm ,Raman spectroscopy ,tissue engineering ,Organic chemistry ,QD241-441 - Abstract
Biomaterials used in tissue engineering and regenerative medicine applications benefit from longitudinal monitoring in a non-destructive manner. Label-free imaging based on fluorescence lifetime imaging (FLIm) and Raman spectroscopy were used to monitor the degree of genipin (GE) cross-linking of antigen-removed bovine pericardium (ARBP) at three incubation time points (0.5, 1.0, and 2.5 h). Fluorescence lifetime decreased and the emission spectrum redshifted compared to that of uncross-linked ARBP. The Raman signature of GE-ARBP was resonance-enhanced due to the GE cross-linker that generated new Raman bands at 1165, 1326, 1350, 1380, 1402, 1470, 1506, 1535, 1574, 1630, 1728, and 1741 cm−1. These were validated through density functional theory calculations as cross-linker-specific bands. A multivariate multiple regression model was developed to enhance the biochemical specificity of FLIm parameters fluorescence intensity ratio (R2 = 0.92) and lifetime (R2 = 0.94)) with Raman spectral results. FLIm and Raman spectroscopy detected biochemical changes occurring in the collagenous tissue during the cross-linking process that were characterized by the formation of a blue pigment which affected the tissue fluorescence and scattering properties. In conclusion, FLIm parameters and Raman spectroscopy were used to monitor the degree of cross-linking non-destructively.
- Published
- 2020
- Full Text
- View/download PDF
48. Shape-Memory Metallopolymer Networks Based on a Triazole–Pyridine Ligand
- Author
-
Josefine Meurer, Julian Hniopek, Stefan Zechel, Marcel Enke, Jürgen Vitz, Michael Schmitt, Jürgen Popp, Martin D. Hager, and Ulrich S. Schubert
- Subjects
shape-memory polymer ,metallopolymer ,triazole-pyridine-metal complex ,stimuli responsive polymer ,Organic chemistry ,QD241-441 - Abstract
Shape memory polymers represent an interesting class of stimuli-responsive polymers. With their ability to memorize and recover their original shape, they could be useful in almost every area of our daily life. We herein present the synthesis of shape-memory metallopolymers in which the switching unit is designed by using bis(pyridine−triazole) metal complexes. The polymer networks were synthesized via free radical polymerization of methyl-, ethyl- or butyl-methacrylate, tri(ethylene glycol) dimethacrylate and a methacrylate moiety of the triazole−pyridine ligand. By the addition of zinc(II) or cobalt(II) acetate it was possible to achieve metallopolymer networks featuring shape-memory abilities. The successful formation of the metal-ligand complex was proven by Fourier transform infrared (FT-IR) spectroscopy and by 1H NMR spectroscopy. Furthermore, the shape-recovery behavior was studied in detailed fashion and even triple-shape memory behavior could be revealed.
- Published
- 2019
- Full Text
- View/download PDF
49. Application of High-Throughput Screening Raman Spectroscopy (HTS-RS) for Label-Free Identification and Molecular Characterization of Pollen
- Author
-
Abdullah S. Mondol, Milind D. Patel, Jan Rüger, Clara Stiebing, Andreas Kleiber, Thomas Henkel, Jürgen Popp, and Iwan W. Schie
- Subjects
raman spectroscopy ,high throughput screening ,pollen detection ,pca-svm ,hca ,Chemical technology ,TP1-1185 - Abstract
Pollen studies play a critical role in various fields of science. In the last couple of decades, replacement of manual identification of pollen by image-based methods using pollen morphological features was a great leap forward, but challenges for pollen with similar morphology remain, and additional approaches are required. Spectroscopy approaches for identification of pollen, such as Raman spectroscopy has potential benefits over traditional methods, due to the investigation of the intrinsic molecular composition of a sample. However, current Raman-based characterization of pollen is complex and time-consuming, resulting in low throughput and limiting the statistical significance of the acquired data. Previously demonstrated high-throughput screening Raman spectroscopy (HTS-RS) eliminates the complexity as well as human interaction by incorporation full automation of the data acquisition process. Here, we present a customization of HTS-RS for pollen identification, enabling sampling of a large number of pollen in comparison to other state-of-the-art Raman pollen investigations. We show that using Raman spectra we are able to provide a preliminary estimation of pollen types based on growth habits using hierarchical cluster analysis (HCA) as well as good taxonomy of 37 different Pollen using principal component analysis-support vector machine (PCA-SVM) with good accuracy even for the pollen specimens sharing similar morphological features. Our results suggest that HTS-RS platform meets the demands for automated pollen detection making it an alternative method for research concerning pollen.
- Published
- 2019
- Full Text
- View/download PDF
50. Raman and infrared spectroscopy reveal that proliferating and quiescent human fibroblast cells age by biochemically similar but not identical processes.
- Author
-
Katharina Eberhardt, Christian Matthäus, Shiva Marthandan, Stephan Diekmann, and Jürgen Popp
- Subjects
Medicine ,Science - Abstract
Dermal fibroblast cells can adopt different cell states such as proliferation, quiescence, apoptosis or senescence, in order to ensure tissue homeostasis. Proliferating (dividing) cells pass through the phases of the cell cycle, while quiescent and senescent cells exist in a non-proliferating cell cycle-arrested state. However, the reversible quiescence state is in contrast to the irreversible senescence state. Long-term quiescent cells transit into senescence indicating that cells age also when not passing through the cell cycle. Here, by label-free in vitro vibrational spectroscopy, we studied the biomolecular composition of quiescent dermal fibroblast cells and compared them with those of proliferating and senescent cells. Spectra were examined by multivariate statistical analysis using a PLS-LDA classification model, revealing differences in the biomolecular composition between the cell states mainly associated with protein alterations (variations in the side chain residues of amino acids and protein secondary structure), but also within nucleic acids and lipids. We observed spectral changes in quiescent compared to proliferating cells, which increased with quiescence cultivation time. Raman and infrared spectroscopy, which yield complementary biochemical information, clearly distinguished contact-inhibited from serum-starved quiescent cells. Furthermore, the spectra displayed spectral differences between quiescent cells and proliferating cells, which had recovered from quiescence. This became more distinct with increasing quiescence cultivation time. When comparing proliferating, (in particular long-term) quiescent and senescent cells, we found that Raman as well as infrared spectroscopy can separate these three cellular states from each other due to differences in their biomolecular composition. Our spectroscopic analysis shows that proliferating and quiescent fibroblast cells age by similar but biochemically not identical processes. Despite their aging induced changes, over long time periods quiescent cells can return into the cell cycle. Finally however, the cell cycle arrest becomes irreversible indicating senescence.
- Published
- 2018
- Full Text
- View/download PDF
Catalog
Discovery Service for Jio Institute Digital Library
For full access to our library's resources, please sign in.