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1. Seroprevalence of SARS-CoV-2 antibodies in social housing areas in Denmark

Author

Fogh, Kamille, Eriksen, Alexandra R. R., Hasselbalch, Rasmus B., Kristensen, Emilie Sofie, Bundgaard, Henning, Nielsen, Susanne D., Jørgensen, Charlotte S., Scharff, Bibi F. S. S., Erikstrup, Christian, Sækmose, Susanne G., Holm, Dorte K., Aagaard, Bitten, Norsk, Jakob, Nielsen, Pernille Brok, Kristensen, Jonas H., Østergaard, Lars, Ellermann-Eriksen, Svend, Andersen, Berit, Nielsen, Henrik, Johansen, Isik S., Wiese, Lothar, Simonsen, Lone, Fischer, Thea K., Folke, Fredrik, Lippert, Freddy, Ostrowski, Sisse R., Ethelberg, Steen, Koch, Anders, Vangsted, Anne-Marie, Krause, Tyra Grove, Fomsgaard, Anders, Nielsen, Claus, Ullum, Henrik, Skov, Robert, and Iversen, Kasper

Published
2022
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2. SARS-CoV-2 antibody prevalence among homeless people and shelter workers in Denmark: a nationwide cross-sectional study

Author

Eriksen, Alexandra R Röthlin, Fogh, Kamille, Hasselbalch, Rasmus B., Bundgaard, Henning, Nielsen, Susanne D., Jørgensen, Charlotte S., Scharff, Bibi F. S. S., Erikstrup, Christian, Sækmose, Susanne G., Holm, Dorte K., Aagaard, Bitten, Kristensen, Jonas H., Bødker, Cecilie A., Norsk, Jakob B., Nielsen, Pernille B., Østergaard, Lars, Ellermann-Eriksen, Svend, Andersen, Berit, Nielsen, Henrik, Johansen, Isik S., Wiese, Lothar, Simonsen, Lone, K.Fischer, Thea, Folke, Fredrik, Lippert, Freddy, Ostrowski, Sisse R., Ethelberg, Steen, Koch, Anders, Vangsted, Anne-Marie, Krause, Tyra, Fomsgaard, Anders, Nielsen, Claus, Ullum, Henrik, Skov, Robert, and Iversen, Kasper

Published
2022
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3. A Cross-Sectional Study of SARS-CoV-2 Antibodies and Risk Factors for Seropositivity in Staff in Day Care Facilities and Preschools in Denmark

Author

Fogh, Kamille, Eriksen, Alexandra R R, Larsen, Tine Graakjær, Hasselbalch, Rasmus B, Bundgaard, Henning, Scharff, Bibi, Nielsen, Susanne D, Jørgensen, Charlotte S, Erikstrup, Christian, Østergaard, Lars, Ellermann-Eriksen, Svend, Andersen, Berit, Nielsen, Henrik, Johansen, Isik S, Wiese, Lothar, Hindhede, Lotte, Mikkelsen, Susan, Sækmose, Susanne G, Aagaard, Bitten, Holm, Dorte K, Harritshøj, Lene, Simonsen, Lone, Fischer, Thea K, Folke, Fredrik, Lippert, Freddy, Ostrowski, Sisse R, Benfield, Thomas, Mølbak, Kåre, Ethelberg, Steen, Koch, Anders, Vangsted, Anne-Marie, Krause, Tyra Grove, Fomsgaard, Anders, Ullum, Henrik, Skov, Robert, Iversen, Kasper, Fogh, Kamille, Eriksen, Alexandra R R, Larsen, Tine Graakjær, Hasselbalch, Rasmus B, Bundgaard, Henning, Scharff, Bibi, Nielsen, Susanne D, Jørgensen, Charlotte S, Erikstrup, Christian, Østergaard, Lars, Ellermann-Eriksen, Svend, Andersen, Berit, Nielsen, Henrik, Johansen, Isik S, Wiese, Lothar, Hindhede, Lotte, Mikkelsen, Susan, Sækmose, Susanne G, Aagaard, Bitten, Holm, Dorte K, Harritshøj, Lene, Simonsen, Lone, Fischer, Thea K, Folke, Fredrik, Lippert, Freddy, Ostrowski, Sisse R, Benfield, Thomas, Mølbak, Kåre, Ethelberg, Steen, Koch, Anders, Vangsted, Anne-Marie, Krause, Tyra Grove, Fomsgaard, Anders, Ullum, Henrik, Skov, Robert, and Iversen, Kasper

Abstract

The aim of this study was to provide information about immunity against COVID-19 along with risk factors and behavior among employees in day care facilities and preschools (DCS) in Denmark. In collaboration with the Danish Union of Pedagogues, during February and March 2021, 47,810 members were offered a point-of-care rapid SARS-CoV-2 antibody test (POCT) at work and were invited to fill in an electronic questionnaire covering COVID-19 exposure. Seroprevalence data from Danish blood donors (total Ig enzyme-linked immunosorbent assay [ELISA]) were used as a proxy for the Danish population. A total of 21,018 (45%) DCS employees completed the questionnaire and reported their POCT result {median age, 44.3 years (interquartile range [IQR], [32.7 to 53.6]); females, 84.1%}, of which 20,267 (96.4%) were unvaccinated and included in analysis. A total of 1,857 (9.2%) participants tested seropositive, significantly higher than a seroprevalence at 7.6% (risk ratio [RR], 1.2; 95% confidence interval [CI], 1.14 to 1.27) among 40,541 healthy blood donors (median age, 42 years [IQR, 28 to 53]; males, 51.3%). Exposure at work (RR, 2.9; 95% CI, 2.3 to 3.6) was less of a risk factor than exposure within the household (RR, 12.7; 95% CI, 10.2 to 15.8). Less than 25% of participants reported wearing face protection at work. Most of the participants expressed some degree of fear of contracting COVID-19 both at work and outside work. SARS-CoV-2 seroprevalence was slightly higher in DCS staff than in blood donors, but possible exposure at home was associated with a higher risk than at work. DCS staff expressed fear of contracting COVID-19, though there was limited use of face protection at work. IMPORTANCE Identifying at-risk groups and evaluating preventive interventions in at-risk groups is imperative for the ongoing pandemic as well as for the control of future epidemics. Although DCS staff have a much higher risk of being infected within their own household than at their workplace

Published
2023

6. A Cross-Sectional Study of SARS-CoV-2 Antibodies and Risk Factors for Seropositivity in Staff in Day Care Facilities and Preschools in Denmark

Author

Fogh, Kamille, primary, Eriksen, Alexandra R. R., additional, Larsen, Tine Graakjær, additional, Hasselbalch, Rasmus B., additional, Bundgaard, Henning, additional, Scharff, Bibi F. S. S., additional, Nielsen, Susanne D., additional, Jørgensen, Charlotte S., additional, Erikstrup, Christian, additional, Østergaard, Lars, additional, Ellermann-Eriksen, Svend, additional, Andersen, Berit, additional, Nielsen, Henrik, additional, Johansen, Isik S., additional, Wiese, Lothar, additional, Hindhede, Lotte, additional, Mikkelsen, Susan, additional, Sækmose, Susanne G., additional, Aagaard, Bitten, additional, Holm, Dorte K., additional, Harritshøj, Lene, additional, Simonsen, Lone, additional, Fischer, Thea K., additional, Folke, Fredrik, additional, Lippert, Freddy, additional, Ostrowski, Sisse R., additional, Benfield, Thomas, additional, Mølbak, Kåre, additional, Ethelberg, Steen, additional, Koch, Anders, additional, Vangsted, Anne-Marie, additional, Krause, Tyra Grove, additional, Fomsgaard, Anders, additional, Ullum, Henrik, additional, Skov, Robert, additional, and Iversen, Kasper, additional

Published
2022
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7. Persistent Symptoms and Sequelae After Severe Acute Respiratory Syndrome Coronavirus 2 Infection Not Requiring Hospitalization: Results From Testing Denmark, a Danish Cross-sectional Survey

Author

van Wijhe, Maarten, primary, Fogh, Kamille, additional, Ethelberg, Steen, additional, Karmark Iversen, Kasper, additional, Nielsen, Henrik, additional, Østergaard, Lars, additional, Andersen, Berit, additional, Bundgaard, Henning, additional, Jørgensen, Charlotte S, additional, Scharff, Bibi F SS, additional, Ellermann-Eriksen, Svend, additional, Johansen, Isik S, additional, Fomsgaard, Anders, additional, Grove Krause, Tyra, additional, Wiese, Lothar, additional, Fischer, Thea K, additional, Mølbak, Kåre, additional, Benfield, Thomas, additional, Folke, Fredrik, additional, Lippert, Freddy, additional, Ostrowski, Sisse R, additional, Koch, Anders, additional, Erikstrup, Christian, additional, Vangsted, Anne-Marie, additional, Sørensen, Anna Irene Vedel, additional, Ullum, Henrik, additional, Skov, Robert Leo, additional, Simonsen, Lone, additional, and Nielsen, Susanne Dam, additional

Published
2022
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11. Additional file 2 of SARS-CoV-2 antibody prevalence among homeless people and shelter workers in Denmark: a nationwide cross-sectional study

Author

Eriksen, Alexandra R Röthlin, Fogh, Kamille, Hasselbalch, Rasmus B., Bundgaard, Henning, Nielsen, Susanne D., Jørgensen, Charlotte S., Scharff, Bibi F. S. S., Erikstrup, Christian, Sækmose, Susanne G., Holm, Dorte K., Aagaard, Bitten, Kristensen, Jonas H., Bødker, Cecilie A., Norsk, Jakob B., Nielsen, Pernille B., Østergaard, Lars, Ellermann-Eriksen, Svend, Andersen, Berit, Nielsen, Henrik, Johansen, Isik S., Wiese, Lothar, Simonsen, Lone, K.Fischer, Thea, Folke, Fredrik, Lippert, Freddy, Ostrowski, Sisse R., Ethelberg, Steen, Koch, Anders, Vangsted, Anne-Marie, Krause, Tyra, Fomsgaard, Anders, Nielsen, Claus, Ullum, Henrik, Skov, Robert, and Iversen, Kasper

Subjects
Data_FILES
Abstract

Additional file 2.

Published
2022
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12. Additional file 1 of SARS-CoV-2 antibody prevalence among homeless people and shelter workers in Denmark: a nationwide cross-sectional study

Author

Eriksen, Alexandra R Röthlin, Fogh, Kamille, Hasselbalch, Rasmus B., Bundgaard, Henning, Nielsen, Susanne D., Jørgensen, Charlotte S., Scharff, Bibi F. S. S., Erikstrup, Christian, Sækmose, Susanne G., Holm, Dorte K., Aagaard, Bitten, Kristensen, Jonas H., Bødker, Cecilie A., Norsk, Jakob B., Nielsen, Pernille B., Østergaard, Lars, Ellermann-Eriksen, Svend, Andersen, Berit, Nielsen, Henrik, Johansen, Isik S., Wiese, Lothar, Simonsen, Lone, K.Fischer, Thea, Folke, Fredrik, Lippert, Freddy, Ostrowski, Sisse R., Ethelberg, Steen, Koch, Anders, Vangsted, Anne-Marie, Krause, Tyra, Fomsgaard, Anders, Nielsen, Claus, Ullum, Henrik, Skov, Robert, and Iversen, Kasper

Subjects
Data_FILES
Abstract

Additional file 1.

Published
2022
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13. Estimation of the Seroprevalence and Infection Fatality Rate of the SARS-CoV-2 Omicron Variant Using Antibody Screening of Danish Blood Donors

Author

Erikstrup, Christian, primary, Damkjær Laksafoss, Anna, additional, Gladov, Josephine, additional, Agergård Kaspersen, Kathrine, additional, Mikkelsen, Susan, additional, Hindhede, Lotte, additional, Kjærgaard Boldsen, Jens, additional, Winther Jørgensen, Signe, additional, Ethelberg, Steen, additional, Kinggaard Holm, Dorte, additional, Topholm, Mie, additional, Nissen, Janna, additional, Schwinn, Michael, additional, Brodersen, Thorsten, additional, Mikkelsen, Christina, additional, Gjørup Sækmose, Susanne, additional, Sørensen, Erik, additional, Holm Harritshøj, Lene, additional, Aagaard, Bitten, additional, Dinh, Khoa Manh, additional, Busch, Michael, additional, Jørgensen, Charlotte S., additional, Grove Krause, Tyra, additional, Ullum, Henrik, additional, Ostrowski, Sisse Rye, additional, Espenhain, Laura, additional, and Pedersen, Ole Birger, additional

Published
2022
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14. Persistent Symptoms and Sequelae after SARS-CoV-2 Infection Not Requiring Hospitalization: Results from Testing Denmark, a Danish Cross-Sectional Survey

Author

van Wijhe, Maarten, primary, Fogh, Kamille, additional, Ethelberg, Steen, additional, Iversen, Kasper Karmark, additional, Nielsen, Henrik, additional, Østergaard, Lars Jørgen, additional, Andersen, Berit, additional, Bundgaard, Henning, additional, Jørgensen, Charlotte S., additional, Scharff, Bibi FSS, additional, Ellermann-Eriksen, Svend, additional, Johansen, Isik S., additional, Fomsgaard, Anders, additional, Grove Krause, Tyra, additional, Wiese, Lothar, additional, Fischer, Thea K., additional, Mølbak, Kåre, additional, Benfield, Thomas, additional, Folke, Fredrik, additional, Lippert, Freddy K., additional, Ostrowski, Sisse Rye, additional, Koch, Anders, additional, Erikstrup, Christian, additional, Vangsted, Anne-Marie, additional, Sørensen, Anna Irene Vedel, additional, Ullum, Henrik, additional, Skov, Robert Leo, additional, Simonsen, Lone, additional, and Nielsen, Susanne Dam, additional

Published
2022
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15. Testing Denmark: a Danish Nationwide Surveillance Study of COVID-19

Author

Fogh, Kamille, primary, Strange, Jarl E., additional, Scharff, Bibi F. S. S., additional, Eriksen, Alexandra R. R., additional, Hasselbalch, Rasmus B., additional, Bundgaard, Henning, additional, Nielsen, Susanne D., additional, Jørgensen, Charlotte S., additional, Erikstrup, Christian, additional, Norsk, Jakob, additional, Nielsen, Pernille Brok, additional, Kristensen, Jonas H., additional, Østergaard, Lars, additional, Ellermann-Eriksen, Svend, additional, Andersen, Berit, additional, Nielsen, Henrik, additional, Johansen, Isik S., additional, Wiese, Lothar, additional, Simonsen, Lone, additional, Fischer, Thea K., additional, Folke, Fredrik, additional, Lippert, Freddy, additional, Ostrowski, Sisse R., additional, Benfield, Thomas, additional, Mølbak, Kåre, additional, Ethelberg, Steen, additional, Koch, Anders, additional, Sönksen, Ute Wolff, additional, Vangsted, Anne-Marie, additional, Krause, Tyra Grove, additional, Fomsgaard, Anders, additional, Ullum, Henrik, additional, Skov, Robert, additional, and Iversen, Kasper, additional

Published
2021
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16. Persistent Symptoms and Sequelae After Severe Acute Respiratory Syndrome Coronavirus 2 Infection Not Requiring Hospitalization: Results From Testing Denmark, a Danish Cross-sectional Survey.

Author

Wijhe, Maarten van, Fogh, Kamille, Ethelberg, Steen, Iversen, Kasper Karmark, Nielsen, Henrik, Østergaard, Lars, Andersen, Berit, Bundgaard, Henning, Jørgensen, Charlotte S, Scharff, Bibi F SS, Ellermann-Eriksen, Svend, Johansen, Isik S, Fomsgaard, Anders, Krause, Tyra Grove, Wiese, Lothar, Fischer, Thea K, Mølbak, Kåre, Benfield, Thomas, Folke, Fredrik, and Lippert, Freddy

Subjects
COVID-19, SARS-CoV-2, TASTE disorders, CORONAVIRUS diseases, POST-acute COVID-19 syndrome, SYMPTOMS
Abstract

Background Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection is associated with persistent symptoms ("long COVID"). We assessed the burden of long COVID among nonhospitalized adults with polymerase chain reaction (PCR)–confirmed SARS-CoV-2 infection. Methods In the fall of 2020, a cross-sectional survey was performed in the adult Danish general population. This included a self-administered point-of-care test for SARS-CoV-2 antibodies, the Short Form Health Survey (SF-12), and coronavirus disease 2019 (COVID-19)–associated symptom questions. Nonhospitalized respondents with a positive SARS-CoV-2 PCR test ≥12 weeks before the survey (cases) were matched (1:10) to seronegative controls on age, sex, and body mass index. Propensity score–weighted odds ratios (ORs) and ORs for risk factors were estimated for each health outcome. Results In total, 742 cases and 7420 controls were included. The attributable risk of at least 1 long-COVID symptom was 25.0 per 100 cases (95% confidence interval [CI], 22.2–27.4). Compared to controls, cases reported worse general health (OR, 5.9 [95% CI, 5.0–7.0]) and had higher odds for a broad range of symptoms, particularly loss of taste (OR, 11.8 [95% CI, 9.5–14.6]) and smell (OR, 11.2 [95% CI, 9.1–13.9]). Physical and Mental Component Summary scores were also significantly reduced with differences of −2.5 (95% CI, −3.1 to −1.8) and −2.0 (95% CI, −2.7 to −1.2), respectively. Female sex and severity of initial infection were major risk factors for long COVID. Conclusions Nonhospitalized SARS-CoV-2 PCR–positive individuals had significantly reduced physical and mental health, and 1 in 4 reported persistence of at least 1 long-COVID symptom. [ABSTRACT FROM AUTHOR]

Published
2023
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17. Comparison of sixteen serological SARS-CoV-2 immunoassays in sixteen clinical laboratories

Author

Harritshøj, Lene H, Gybel-Brask, Mikkel, Afzal, Shoaib, Kamstrup, Pia R, Jørgensen, Charlotte S, Thomsen, Marianne Kragh, Hilsted, Linda, Friis-Hansen, Lennart, Szecsi, Pal B, Pedersen, Lise, Nielsen, Lene, Hansen, Cecilie B, Garred, Peter, Korsholm, Trine-Line, Mikkelsen, Susan, Nielsen, Kirstine O, Møller, Bjarne K, Hansen, Anne T, Iversen, Kasper K, Nielsen, Pernille B, Hasselbalch, Rasmus B, Fogh, Kamille, Norsk, Jakob B, Kristensen, Jonas Henrik, Schønning, Kristian, Kirkby, Nikolai S, Nielsen, Alex C Y, Landsy, Lone H, Loftager, Mette, Holm, Dorte K, Nilsson, Anna C, Sækmose, Susanne G, Grum-Schwensen, Birgitte, Aagaard, Bitten, Jensen, Thøger G, Nielsen, Dorte M, Ullum, Henrik, Dessau, Ram B C, Harritshøj, Lene H, Gybel-Brask, Mikkel, Afzal, Shoaib, Kamstrup, Pia R, Jørgensen, Charlotte S, Thomsen, Marianne Kragh, Hilsted, Linda, Friis-Hansen, Lennart, Szecsi, Pal B, Pedersen, Lise, Nielsen, Lene, Hansen, Cecilie B, Garred, Peter, Korsholm, Trine-Line, Mikkelsen, Susan, Nielsen, Kirstine O, Møller, Bjarne K, Hansen, Anne T, Iversen, Kasper K, Nielsen, Pernille B, Hasselbalch, Rasmus B, Fogh, Kamille, Norsk, Jakob B, Kristensen, Jonas Henrik, Schønning, Kristian, Kirkby, Nikolai S, Nielsen, Alex C Y, Landsy, Lone H, Loftager, Mette, Holm, Dorte K, Nilsson, Anna C, Sækmose, Susanne G, Grum-Schwensen, Birgitte, Aagaard, Bitten, Jensen, Thøger G, Nielsen, Dorte M, Ullum, Henrik, and Dessau, Ram B C

Abstract

Serological SARS-CoV-2 assays are needed to support clinical diagnosis and epidemiological investigations. Recently, assays for large-scale detection of total antibodies (total-Ab) and immunoglobulin (Ig) G and M against SARS-CoV-2 antigens have been developed, but there are limited data on the diagnostic accuracy of these assays. This study was a Danish national collaboration and evaluated fifteen commercial and one in-house anti-SARS-CoV-2 assays in sixteen laboratories. Sensitivity was evaluated using 150 samples from individuals with asymptomatic, mild or moderate COVID-19; nonhospitalized or hospitalized, confirmed by nucleic acid amplification tests (NAAT), collected 13-73 days either from symptom onset or from positive NAAT (patients without symptoms). Specificity and cross reactivity were evaluated in samples collected prior to the SARS-CoV-2 epidemic from >586 blood donors and patients with autoimmune diseases, cytomegalovirus or Epstein-Barr virus infections and acute viral infections. A specificity of ≥99% was achieved by all total-Ab and IgG assays except one, Diasorin/LiaisonXL-IgG (97.2%). Sensitivities in descending order were: Wantai/ELISA total-Ab (96.7%), CUH-NOVO/in-house ELISA total-Ab (96.0%), Ortho/Vitros total-Ab (95.3%), YHLO/iFlash-IgG (94.0%), Ortho/Vitros-IgG (93.3%), Siemens/Atellica total-Ab (93.2%), Roche/Elecsys total-Ab (92.7%), Abbott/Architect-IgG (90.0%), Abbott/Alinity-IgG (median 88.0%), Diasorin/LiaisonXL-IgG (median 84.6%), Siemens/Vista total-Ab (81.0%), Euroimmun/ELISA-IgG (78.0%), and Snibe/Maglumi-IgG (median 78.0%). However, confidence intervals overlapped for several assays. The IgM results were variable, with the Wantai/ELISA-IgM showing the highest sensitivity (82.7%) and specificity (99%). The rate of seropositivity increased with time from symptom onset and symptom severity.

Published
2021

19. SARS-CoV-2 antibody prevalence among homeless people, sex workers and shelter workers in Denmark: a nationwide cross-sectional study

Author

Röthlin Eriksen, Alexandra R, primary, Fogh, Kamille, additional, Hasselbalch, Rasmus B, additional, Bundgaard, Henning, additional, Nielsen, Susanne D, additional, Jørgensen, Charlotte S, additional, Scharff, Bibi FSS, additional, Erikstrup, Christian, additional, Sækmose, Susanne G., additional, Holm, Dorte K, additional, Aagaard, Bitten, additional, Kristensen, Jonas H, additional, Bødker, Cecilie A, additional, Norsk, Jakob, additional, Nielsen, Pernille Brok, additional, Østergaard, Lars, additional, Ellermann-Eriksen, Svend, additional, Andersen, Berit, additional, Nielsen, Henrik, additional, Johansen, Isik S., additional, Wiese, Lothar, additional, Simonsen, Lone, additional, K.Fischer, Thea, additional, Folke, Fredrik, additional, Lippert, Freddy, additional, Ostrowski, Sisse R, additional, Ethelberg, Steen, additional, Koch, Anders, additional, Vangsted, Anne-Marie, additional, Krause, Tyra, additional, Fomsgaard, Anders, additional, Nielsen, Claus, additional, Ullum, Henrik, additional, Skov, Robert, additional, and Iversen, Kasper, additional

Published
2021
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20. Seroprevalence of SARS-CoV-2 antibodies in social housing areas in Denmark

Author

Fogh, Kamille, primary, Eriksen, Alexandra RR, additional, Hasselbalch, Rasmus B, additional, Kristensen, Emilie Sofie, additional, Bundgaard, Henning, additional, Nielsen, Susanne D, additional, Jørgensen, Charlotte S, additional, Scharff, Bibi FSS, additional, Erikstrup, Christian, additional, Sækmose, Susanne G, additional, Holm, Dorte K, additional, Aagaard, Bitten, additional, Norsk, Jakob, additional, Nielsen, Pernille Brok, additional, Kristensen, Jonas H, additional, Østergaard, Lars, additional, Ellermann-Eriksen, Svend, additional, Andersen, Berit, additional, Nielsen, Henrik, additional, Johansen, Isik S, additional, Wiese, Lothar, additional, Simonsen, Lone, additional, Fischer, Thea K., additional, Folke, Fredrik, additional, Lippert, Freddy, additional, Ostrowski, Sisse R, additional, Ethelberg, Steen, additional, Koch, Anders, additional, Vangsted, Anne-Marie, additional, Krause, Tyra Grove, additional, Fomsgaard, Anders, additional, Nielsen, Claus, additional, Ullum, Henrik, additional, Skov, Robert, additional, and Iversen, Kasper, additional

Published
2021
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21. Comparison of 16 Serological SARS-CoV-2 Immunoassays in 16 Clinical Laboratories

Author

Harritshøj, Lene H., primary, Gybel-Brask, Mikkel, additional, Afzal, Shoaib, additional, Kamstrup, Pia R., additional, Jørgensen, Charlotte S., additional, Thomsen, Marianne Kragh, additional, Hilsted, Linda, additional, Friis-Hansen, Lennart, additional, Szecsi, Pal B., additional, Pedersen, Lise, additional, Nielsen, Lene, additional, Hansen, Cecilie B., additional, Garred, Peter, additional, Korsholm, Trine-Line, additional, Mikkelsen, Susan, additional, Nielsen, Kirstine O., additional, Møller, Bjarne K., additional, Hansen, Anne T., additional, Iversen, Kasper K., additional, Nielsen, Pernille B., additional, Hasselbalch, Rasmus B., additional, Fogh, Kamille, additional, Norsk, Jakob B., additional, Kristensen, Jonas Henrik, additional, Schønning, Kristian, additional, Kirkby, Nikolai S., additional, Nielsen, Alex C. Y., additional, Landsy, Lone H., additional, Loftager, Mette, additional, Holm, Dorte K., additional, Nilsson, Anna C., additional, Sækmose, Susanne G., additional, Grum-Schwensen, Birgitte, additional, Aagaard, Bitten, additional, Jensen, Thøger G., additional, Nielsen, Dorte M., additional, Ullum, Henrik, additional, and Dessau, Ram B., additional

Published
2021
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22. Comparison of sixteen serological SARS-CoV-2 immunoassays in sixteen clinical laboratories

Author

Harritshøj, Lene H., primary, Gybel-Brask, Mikkel, additional, Afzal, Shoaib, additional, Kamstrup, Pia R., additional, Jørgensen, Charlotte S., additional, Thomsen, Marianne Kragh, additional, Hilsted, Linda, additional, Friis-Hansen, Lennart, additional, Szecsi, Pal B., additional, Pedersen, Lise, additional, Nielsen, Lene, additional, Hansen, Cecilie B., additional, Garred, Peter, additional, Korsholm, Trine-Line, additional, Mikkelsen, Susan, additional, Nielsen, Kirstine O., additional, Møller, Bjarne K., additional, Hansen, Anne T., additional, Iversen, Kasper K., additional, Nielsen, Pernille B., additional, Hasselbalch, Rasmus B., additional, Fogh, Kamille, additional, Norsk, Jakob B., additional, Kristensen, Jonas Henrik, additional, Schønning, Kristian, additional, Kirkby, Nikolai S., additional, Nielsen, Alex C. Y., additional, Landsy, Lone H., additional, Loftager, Mette, additional, Holm, Dorte K., additional, Nilsson, Anna C., additional, Sækmose, Susanne G., additional, Grum-Schwensen, Birgitte, additional, Aagaard, Bitten, additional, Jensen, Thøger G., additional, Nielsen, Dorte M., additional, Ullum, Henrik, additional, and Dessau, Ram B.C., additional

Published
2020
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27. 926 Specific Antibody Response to Two Pneumococcal Vaccines in Crohn's Disease Patients Treated With Immunosuppressive Drugs Alone or in Combination With Biological Therapy

Author

Kantsø, Bjørn, primary, Halkjaer, Sofie, additional, Thomsen, Ole, additional, Belard, Erika, additional, Gottschalck, Ida Benedikte, additional, Jørgensen, Charlotte S., additional, Slotved, Hans-Christian, additional, Ingels, Helene Andrea S., additional, and Petersen, Andreas M., additional

Published
2015
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29. Dimerization and oligomerization of the chaperone calreticulin

Author

Jørgensen, Charlotte S, Ryder, L Rebekka, Steinø, Anne, Højrup, Peter, Hansen, Jesper, Beyer, N Helena, Heegaard, Niels H H, and Houen, Gunnar

Subjects
Silver Staining, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization, Electrophoresis, Polyacrylamide Gel, Hydrogen-Ion Concentration, Calreticulin, Crystallography, X-Ray, Dimerization, Heat
Abstract

Udgivelsesdato: 2003-Oct The chaperone calreticulin is a highly conserved eukaryotic protein mainly located in the endoplasmic reticulum. It contains a free cysteine SH group but does not form disulfide-bridged dimers under physiological conditions, indicating that the SH group may not be fully accessible in the native protein. Using PAGE, urea gradient gel electrophoresis, capillary electrophoresis and MS, we show that dimerization through the SH group can be induced by lowering the pH to 5-6, heating, or under conditions that favour partial unfolding such as urea concentrations above 2.6 m or SDS concentrations above 0.025%. Moreover, we show that calreticulin also has the ability to self-oligomerize through noncovalent interactions at urea concentrations above 2.6 m at pH below 4.6 or above pH 10, at temperatures above 40 degrees C, or in the presence of high concentrations of organic solvents (25%), conditions that favour partial unfolding or an intramolecular local conformational change that allows oligomerization, resulting in a heterogeneous mixture of oligomers consisting of up to 10 calreticulin monomers. The oligomeric calreticulin was very stable, but oligomerization was partially reversed by addition of 8 m urea or 1% SDS, and heat-induced oligomerization could be inhibited by 8 m urea or 1% SDS when present during heating. Comparison of the binding properties of monomeric and oligomeric calreticulin in solid-phase assays showed increased binding to peptides and denatured proteins when calreticulin was oligomerized. Thus, calreticulin shares the ability to self-oligomerize with other important chaperones such as GRP94 and HSP90, a property possibly associated with their chaperone activity.

Published
2003
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30. Protein chemical characterization of Gc globulin (vitamin D-binding protein) isoforms; Gc-1f, Gc-1s and Gc-2.

Author

Christiansen, Maja, Jørgensen, Charlotte S, Laursen, Inga, Hirschberg, Daniel, Højrup, Peter, Houen, Gunnar, Christiansen, Maja, Jørgensen, Charlotte S, Laursen, Inga, Hirschberg, Daniel, Højrup, Peter, and Houen, Gunnar

Abstract

Gc globulin, also called vitamin D-binding protein, is a plasma protein involved in the extracellular actin-scavenger system, vitamin D transport and possibly also other biological activities. Low levels of Gc globulin have been found to correlate with multiple organ failure and non-survival of patients with fulminant hepatic failure and trauma. Here, we characterize the dominant isoforms of plasma-derived Gc globulin from Cohn fraction IV paste with respect to amino acid sequence and posttranslational modifications. Gc globulin was purified in large scale and the isoforms separated by ion exchange chromatography. The separated isoforms and several commercial preparations of individual isoforms were characterized by mass spectrometry. This revealed that the major isoforms were non-glycosylated. Compared to the Gc-1f isoform the other dominating isoforms represented an Asp/Glu substitution (Gc-1s) and a Thr/Lys substitution (Gc-2) in agreement with DNA sequencing studies. The commercial preparations were found to represent mainly one or two isoforms. An O-linked glycan with a mass of 656 Da and terminating with a sialic acid residue was detected on a minor proportion of Gc globulin molecules.

Published
2007
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31. Conformational stability of calreticulin

Author

Jørgensen, Charlotte S, Trandum, Christa, Larsen, Nanna Brink, Ryder, L Rebekka, Gajhede, Michael, Skov, Lars, Højrup, Peter, Barkholt, Vibeke, Houen, Gunnar, Jørgensen, Charlotte S, Trandum, Christa, Larsen, Nanna Brink, Ryder, L Rebekka, Gajhede, Michael, Skov, Lars, Højrup, Peter, Barkholt, Vibeke, and Houen, Gunnar

Abstract

The conformational stability of calreticulin was investigated. Apparent unfolding temperatures (Tm) increased from 31 degrees C at pH 5 to 51 degrees C at pH 9, but electrophoretic analysis revealed that calreticulin oligomerized instead of unfolding. Structural analyses showed that the single C-terminal alpha-helix was of major importance to the conformational stability of calreticulin.

Published
2005

36. A Cross-Sectional Study of SARS-CoV-2 Antibodies and Risk Factors for Seropositivity in Staff in Day Care Facilities and Preschools in Denmark.

Author

Fogh K, Eriksen ARR, Larsen TG, Hasselbalch RB, Bundgaard H, Scharff BFSS, Nielsen SD, Jørgensen CS, Erikstrup C, Østergaard L, Ellermann-Eriksen S, Andersen B, Nielsen H, Johansen IS, Wiese L, Hindhede L, Mikkelsen S, Sækmose SG, Aagaard B, Holm DK, Harritshøj L, Simonsen L, Fischer TK, Folke F, Lippert F, Ostrowski SR, Benfield T, Mølbak K, Ethelberg S, Koch A, Vangsted AM, Krause TG, Fomsgaard A, Ullum H, Skov R, and Iversen K

Subjects
Adult, Female, Humans, Male, Cross-Sectional Studies, Denmark epidemiology, Risk Factors, SARS-CoV-2, Seroepidemiologic Studies, Antibodies, Viral, COVID-19 epidemiology, Child Day Care Centers, Schools, Faculty
Abstract

The aim of this study was to provide information about immunity against COVID-19 along with risk factors and behavior among employees in day care facilities and preschools (DCS) in Denmark. In collaboration with the Danish Union of Pedagogues, during February and March 2021, 47,810 members were offered a point-of-care rapid SARS-CoV-2 antibody test (POCT) at work and were invited to fill in an electronic questionnaire covering COVID-19 exposure. Seroprevalence data from Danish blood donors (total Ig enzyme-linked immunosorbent assay [ELISA]) were used as a proxy for the Danish population. A total of 21,018 (45%) DCS employees completed the questionnaire and reported their POCT result {median age, 44.3 years (interquartile range [IQR], [32.7 to 53.6]); females, 84.1%}, of which 20,267 (96.4%) were unvaccinated and included in analysis. A total of 1,857 (9.2%) participants tested seropositive, significantly higher than a seroprevalence at 7.6% (risk ratio [RR], 1.2; 95% confidence interval [CI], 1.14 to 1.27) among 40,541 healthy blood donors (median age, 42 years [IQR, 28 to 53]; males, 51.3%). Exposure at work (RR, 2.9; 95% CI, 2.3 to 3.6) was less of a risk factor than exposure within the household (RR, 12.7; 95% CI, 10.2 to 15.8). Less than 25% of participants reported wearing face protection at work. Most of the participants expressed some degree of fear of contracting COVID-19 both at work and outside work. SARS-CoV-2 seroprevalence was slightly higher in DCS staff than in blood donors, but possible exposure at home was associated with a higher risk than at work. DCS staff expressed fear of contracting COVID-19, though there was limited use of face protection at work. IMPORTANCE Identifying at-risk groups and evaluating preventive interventions in at-risk groups is imperative for the ongoing pandemic as well as for the control of future epidemics. Although DCS staff have a much higher risk of being infected within their own household than at their workplace, most are fearful of being infected with COVID-19 or bringing COVID-19 to work. This represents an interesting dilemma and an important issue which should be addressed by public health authorities for risk communication and pandemic planning. This study design can be used in a strategy for ongoing surveillance of COVID-19 immunity or other infections in the population. The findings of this study can be used to assess the need for future preventive interventions in DCS, such as the use of personal protective equipment.

Published
2023
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37. Large-scale purification and characterization of non-glycosylated Gc globulin (vitamin D-binding protein) from plasma fraction IV.

Author

Jørgensen CS, Christiansen M, Laursen I, Krogsøe LB, Højrup P, Blou L, and Houen G

Subjects
Chromatography, Gel, Chromatography, Ion Exchange, Electrophoresis methods, Humans, Liver Failure, Acute drug therapy, Multiple Organ Failure drug therapy, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization, Vitamin D-Binding Protein blood, Vitamin D-Binding Protein chemistry, Vitamin D-Binding Protein therapeutic use, Vitamin D-Binding Protein isolation & purification
Abstract

Gc globulin, also called vitamin D-binding protein, is a plasma protein involved in the extracellular actin-scavenger system. Low levels of Gc globulin have been found to correlate with multiple organ failure and nonsurvival of patients with fulminant hepatic failure and trauma. Therefore substitution therapy with Gc globulin might be beneficial for such patients, increasing their chance of survival. In the present study, we describe a large-scale purification process for the production of a virus-safe human plasma-derived Gc globulin from Cohn fraction IV paste. The process includes three ion-exchange-chromatography steps, followed by a gel filtration, and two virus-reduction steps are implemented. The Gc globulin product was characterized with respect to purity, functional activity, glycosylation and, finally, with respect to content of endotoxin. From the results, it can be concluded that human Gc globulin purified from Cohn fraction IV is non-glycosylated. The purified Gc globulin is able to mask the presence of endotoxin by 20%.

Published
2006
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38. Conformational stability of calreticulin.

Author

Jørgensen CS, Trandum C, Larsen N, Ryder LR, Gajhede M, Skov LK, Højrup P, Barkholt V, and Houen G

Subjects
Calcium chemistry, Calcium pharmacology, Calorimetry, Differential Scanning, Cations, Divalent chemistry, Circular Dichroism, Humans, Hydrogen-Ion Concentration, Protein Conformation, Protein Denaturation, Protein Folding, Temperature, Calreticulin chemistry, Calreticulin metabolism
Abstract

The conformational stability of calreticulin was investigated. Apparent unfolding temperatures (Tm) increased from 31 degrees C at pH 5 to 51 degrees C at pH 9, but electrophoretic analysis revealed that calreticulin oligomerized instead of unfolding. Structural analyses showed that the single C-terminal alpha-helix was of major importance to the conformational stability of calreticulin.

Published
2005
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