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Preparation par polymerisation redox en solution des 2-desoxy-6-O-α-D-glucopyranosyl-2-(N-[2-(methacryloyloxy) ethyl] ureido)-acide gluconique et sorbitol. Le rayon de giration, le 2eme coefficient du viriel et la viscosite intrinseque des 2 polymeres sont determines en solution aqueuse de Na 2 SO 4

New amphiphilic poly(vinylsaccharide)s (poly(7) and poly(8)) were synthesized. Various alkylamino substituted mono- and disaccharides 3 and 5 were obtained by reductive amination of reducing mono- and disaccharides with alkylamines. The reaction of these monofunctionalized sugar derivates with 2-isocyanatoethyl methacrylate (2) leads to well-defined monomers (7 and 8) of the urea type. The monomers were polymerized in aqueous solution to high-molecular-weight polymers, using the redox initiator system (NH4)2S2O8/Na2S2O5. The influence of structural variations by changing the hydrophilic sugar moiety or the lipophilic alkyl part was studied by viscometry, light scattering and measurements of the surface tension.

The β-galactosidase from bovine testes (EC 3.2.1.23) promotes the transfer of a galactose unit to glucose or galactose-containing residues in manifold derivatives, establishing β1→3 linkages. The synthesis of several potentially biologically important oligosaccharides β- d -Gal p -(1→3)-α- d -Glc p -(1→2)-β- d -Fru f 2 , β- d -Gal p -(1→3)-β- d -Gal p -(1→4)-α,β- d -Glc p 4 , β- d -Gal p -(1→3)-α- d -Glc p -(1→4)- d -Glc p -ol/Man p -ol 6 , β- d -Gal p -(1→3)-α- d -Glc p -(1→6)-β- d -Fru f 8 , β- d -Gal p -(1→3)-α- d -Glc p -(1→6)-[α- d -Glc p -(1↔2)]-β- d -Fru f 10 , α- d -Gal p -(1→6)-[β- d -Gal p -(1→3)]-α- d -Glc p -(1↔2)-β- d -Fru f 12 , β- d -Gal p -(1→3)-α- d -Glc p -(1↔2)-β- d -Fru f -(1↔2)-β- d -Fru f 14 has been reached in yields between 7 and 44% by implementation of this specific enzyme. In addition, we found that it is feasible to gain high yields without an enzyme-specific buffer and even making upscaled preparation on a gram scale.