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205 results on '"Iwashita, Kazuhiro"'

17. Additional file 4 of Genetic architecture of berry aroma compounds in a QTL (quantitative trait loci) mapping population of interspecific hybrid grapes (Vitis labruscana × Vitis vinifera)

Author

Koyama, Kazuya, Kono, Atsushi, Ban, Yusuke, Bahena-Garrido, Sharon Marie, Ohama, Tomoko, Iwashita, Kazuhiro, Fukuda, Hisashi, and Goto-Yamamoto, Nami

Abstract

Additional file 4: Table S1. Free and bound volatile components identified in ‘Muscat of Alexandria’ and ‘Campbell Early’ berries. Table S2. Meteorological conditions in the Higashihiroshima region during the developmental period of grape berries in 2014 and 2015. Table S5. QTL analysis results showing the hybrid population’s volatile concentrations (Pop AC). Table S6. Newly designed primers used in this study.

Published
2022
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18. Additional file 2 of Genetic architecture of berry aroma compounds in a QTL (quantitative trait loci) mapping population of interspecific hybrid grapes (Vitis labruscana × Vitis vinifera)

Author

Koyama, Kazuya, Kono, Atsushi, Ban, Yusuke, Bahena-Garrido, Sharon Marie, Ohama, Tomoko, Iwashita, Kazuhiro, Fukuda, Hisashi, and Goto-Yamamoto, Nami

Abstract

Additional file 2: Fig. S2. Histograms of volatile concentrations of the hybrid population (Pop AC) for the 2 years. Histograms of 45 volatiles for the 2 years (2014 and 2015) are shown ((a)-(as)). The concentrations of the volatiles are expressed as μg per g of berries. The frequency of a given class for the population is shown on the vertical axis. Arrows indicate the concentration positions of the two-parent cultivars; MA: ‘Muscat of Alexandria’, CE: ‘Campbell Early’. The coefficient of determination between years for each volatile is shown in each histogram’s upper right section.

Published
2022
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19. Additional file 1 of Genetic architecture of berry aroma compounds in a QTL (quantitative trait loci) mapping population of interspecific hybrid grapes (Vitis labruscana × Vitis vinifera)

Author

Koyama, Kazuya, Kono, Atsushi, Ban, Yusuke, Bahena-Garrido, Sharon Marie, Ohama, Tomoko, Iwashita, Kazuhiro, Fukuda, Hisashi, and Goto-Yamamoto, Nami

Abstract

Additional file 1: Fig. S1. Volatile compositions in ‘Muscat of Alexandria’ and ‘Campbell Early’ berries. Both the bound and free volatiles detected were classified into nine chemical groups. Then, the total concentrations of each group averaged over 2 years (a), and the concentrations of individual bound (b) and free (c) monoterpenes of the two cultivars, were shown as pie charts for the comparison.

Published
2022
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20. Additional file 3 of Genetic architecture of berry aroma compounds in a QTL (quantitative trait loci) mapping population of interspecific hybrid grapes (Vitis labruscana × Vitis vinifera)

Author

Koyama, Kazuya, Kono, Atsushi, Ban, Yusuke, Bahena-Garrido, Sharon Marie, Ohama, Tomoko, Iwashita, Kazuhiro, Fukuda, Hisashi, and Goto-Yamamoto, Nami

Abstract

Additional file 3: Fig. S3. Box plot showing the volatiles concentrations for the genotypes of SSR markers and MYB haplotypes nearest to the QTL in the hybrid population (Pop AC). Genotypes of the SSR markers were expressed as allele lengths in bp. Horizontal lines inside boxes show median values, whereas horizontal lines through boxes indicate means. Box height indicate 50% of the data. Different letters (α, β, γ) indicate that the values are significantly different at P < 0.05 by Tukey’s HSD. MA: ‘Muscat of Alexandria’, CE: ‘Campbell Early’.

Published
2022
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27. Proteomic analysis of extracellular proteins from Aspergillus oryzae grown under submerged and solid-state culture conditions

Author

Oda, Ken, Kakizono, Dararat, Yamada, Osamu, Iefuji, Haruyuki, Akita, Osamu, and Iwashita, Kazuhiro

Subjects
Aspergillus -- Growth, Extracellular matrix -- Research, Proteomics -- Research, Bacterial proteins -- Structure, Bacterial proteins -- Chemical properties, Company growth, Biological sciences
Abstract

A comparative proteome analysis of extracellular proteins is carried out under solid-state and submerged culture conditions and protein secretion profiles are determined. A Northern analysis of seven genes from the four groups have indicated that the secretion of alpha-amylase (TAA) and beta-glucosidase (Bgl) are regulated by trapping these proteins in the cell wall in submerged culture and that secretion of glucoamylase A (GlaA) and xylanase G2 (XynG2) are regulated at the posttranscriptional level in the solid-state culture.

Published
2006

28. Genome sequencing and analysis of Aspergillus oryzae

Author

Machida, Masayuki, Asai, Kiyoshi, Sano, Motoaki, Tanaka, Toshihiro, Kumagai, Toshitaka, Terai, Goro, Kusumoto, Ken-Ichi, Arima, Toshihide, Akita, Osamu, Kashiwagi, Yutaka, Abe, Keietsu, Gomi, Katsuya, Horiuchi, Hiroyuki, Kitamoto, Katsuhiko, Kobayashi, Tetsuo, Takeuchi, Michio, Denning, David W., Galagan, James E., Nierman, William C., Yu, Jiujiang, Archer, David B., Bennett, Joan W., Bhatnagar, Deepak, Cleveland, Thomas E., Fedorova, Natalie D., Gotoh, Osamu, Horikawa, Hiroshi, Hosoyama, Akira, Ichinomiya, Masayuki, Igarashi, Rie, Iwashita, Kazuhiro, Juvvadi, Praveen Rao, Kato, Masashi, Kato, Yumiko, Kin, Taishin, Kokubun, Akira, Maeda, Hiroshi, Maeyama, Noriko, Maruyama, Jun-ichi, Nagasaki, Hideki, Nakajima, Tasuku, Oda, Ken, Okada, Kinya, Paulsen, Ian, Sakamoto, Kazutoshi, Sawano, Toshihiko, Takahashi, Mikio, Takase, Kumiko, Terabayashi, Yasunobu, Wortman, Jennifer R., Yamada, Osamu, Yamagata, Youhei, Anazawa, Hideharu, Hata, Yoji, Koide, Yoshinao, Komori, Takashi, Koyama, Yasuji, Minetoki, Toshitaka, Suharnan, Sivasundaram, Tanaka, Akimitsu, Isono, Katsumi, Kuhara, Satoru, Ogasawara, Naotake, and Kikuchi, Hisashi

Subjects
Environmental issues, Science and technology, Zoology and wildlife conservation
Abstract

Author(s): Masayuki Machida (corresponding author) [1]; Kiyoshi Asai [2]; Motoaki Sano [1]; Toshihiro Tanaka [3]; Toshitaka Kumagai [2]; Goro Terai [2, 20]; Ken-Ichi Kusumoto [4]; Toshihide Arima [5]; Osamu Akita [...]

Published
2005
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29. The AWA1 gene is required for the foam-forming phenotype and cell surface hydrophobicity of sake yeast

Author

Shimoi, Hitoshi, Sakamoto, Kazutoshi, Okuda, Masaki, Atthi, Ratchanee, Iwashita, Kazuhiro, and Ito, Kiyoshi

Subjects
Microbiological research -- Analysis, Yeast -- Genetic aspects, Phenotype -- Genetic aspects, Plasma membranes -- Genetic aspects, Fermentation -- Physiological aspects, Biological sciences
Abstract

Research has been conducted on the sake yeasts which form foam layer during the fermentation process. The results indicate that the cell wall protein Awa1p is responsible for the foam formation and cell surface hydrophobicity of this yeast.

Published
2002

32. The bglA gene of Aspergillus kawachii encodes both extracellular and cell wall-bound beta-glucosidases

Author

Iwashita, Kazuhiro, Nagahara, Tatsuya, Kimura, Hitoshi, Takano, Makoto, Shimoi, Hitoshi, and Ito, Kiyoshi

Subjects
Molecular microbiology -- Research, Aspergillus -- Genetic aspects, Glucosidases -- Genetic aspects, Biological sciences
Abstract

The gene encoding beta-glucosidase in Aspergillus kawachii has been cloned and its sequence analyzed. It was found that both of the extracellular beta-glucosidases and the cell wall-bound glucosidase are encoded by the gene, now designated bglA.

Published
1999

34. Analysis of metabolites in Japanese alcoholic beverage sake made from the sake rice Koshitanrei

Author

Ichikawa, Eri, Shougo Hirata, Hata, Yuko, Yazawa, Hisashi, Tamura, Hiroyasu, Mitsuoki Kaneoke, Iwashita, Kazuhiro, and Hirata, Dai

Subjects
viruses, food and beverages, skin and connective tissue diseases
Abstract

In sake brewing, the steamed rice is used in two ways, added to sake-mash (as kake-mai) and making koji. The rice is an important determinant for the quality of sake, as the metabolites in sake affect its taste/aroma. The sake rice Koshitanrei (KOS) was developed in Niigata Prefecture by genetically crossing two sake rice, Gohyakumangoku and Yamadanishiki. However, the metabolites in sake from KOS have not been analyzed. Here, to investigate the characteristic metabolites in sake from KOS, we performed two types of small-scale sake-fermentation tests changing only the rice used for kake-mai or total rice (both kake-mai and koji) by these three rice cultivars and examined the effect of KOS on sake metabolites by the metabolome analysis method using UPLC-QTOF-MS. We identified the peaks/metabolites, whose intensity in sake from KOS was higher/lower than those from the other cultivars. The brewing properties of KOS were partially characterized by this analysis. Metabolites in sake affect the taste and aroma. Specific metabolites affected by the sake rice Koshitanrei were identified by sake metabolome analysis method.

Published
2019
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44. Whole-genome sequencing of sake yeast Saccharomyces cerevisiae Kyokai no.7

Author

Akao, Takeshi, Yashiro, Isao, Hosoyama, Akira, Kitagaki, Hiroshi, Horikawa, Hiroshi, Watanabe, Daisuke, Ando, Yoshinori, Harashima, Satoshi, Inoue, Toyohisa, Inoue, Yoshiharu, Kajiwara, Susumu, Kitamoto, Katsuhiko, Kitamoto, Noriyuki, Kobayashi, Osamu, Kuhara, Satoru, Masubuchi, Takashi, Mizoguchi, Haruhiko, Nakao, Yoshihiro, Nakazato, Atsumi, Namise, Masahiro, Oba, Takahiro, Ogata, Tomoo, Ohta, Akinori, Sato, Masahide, Shibasaki, Seiji, Takatsume, Yoshifumi, Tanimoto, Shota, Tsuboi, Hirokazu, Nishimura, Akira, Yoda, Koji, Ishikawa, Takeaki, Iwashita, Kazuhiro, Fujita, Nobuyuki, and Shimoi, Hitoshi

Subjects
genome sequence, sake yeast, loss of heterozygosity, Saccharomyces cerevisiae, diploid
Published
2011

45. Characterization of amylolytic enzyme overproducing mutant of Aspergillus luchuensis obtained by ion beam mutagenesis

Author

Kojo, Toshihiro, primary, Kadooka, Chihiro, additional, Komohara, Marisa, additional, Onitsuka, Shiori, additional, Tanimura, Miya, additional, Muroi, Yukiko, additional, Kurazono, Shugo, additional, Shiraishi, Yohei, additional, Oda, Ken, additional, Iwashita, Kazuhiro, additional, Onoue, Masahira, additional, Okutsu, Kayu, additional, Yoshizaki, Yumiko, additional, Takamine, Kazunori, additional, Futagami, Taiki, additional, Mori, Kazuki, additional, and Tamaki, Hisanori, additional

Published
2017
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49. Effect of kojistarter on metabolites in Japanese alcoholic beverage sake made from the sake rice Koshitanrei

Author

Ichikawa, Eri, Hirata, Shougo, Hata, Yuko, Yazawa, Hisashi, Tamura, Hiroyasu, Kaneoke, Mitsuoki, Iwashita, Kazuhiro, and Hirata, Dai

Abstract

ABSTRACTIn sake brewing, the steamed rice is used in 2 ways, added to sake-mash and making rice-koji. Rice-kojiis made from the steamed rice by using kojistarter, and its quality is an important determinant of the aroma/taste of sake. The sake rice Koshitanrei(KOS) was developed in Niigata Prefecture by crossing 2 sake rice varieties, Gohyakumangokuand Yamadanishiki. Recently, we reported the characteristic components/metabolites in sake made from KOS by conducting metabolome analysis using UPLC-QTOF-MS. In this study, to investigate the effect of kojistarter and sake rice cultivars on the sake metabolites, we performed small-scale sake-making tests using the above 3 rice cultivars and 3 kojistarters. Finally, we demonstrated that some of the characteristic components/metabolites of sake from KOS are affected by the kojistarter. Thus, in addition to rice cultivar, kojistarter plays an important role for establishment/maintenance of the quality of the final product.

Published
2020
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50. Inter-strain expression of sequence-diverse HET domain genes severely inhibits growth of Aspergillus oryzae.

Author

Mori, Noriko, Katayama, Takuya, Saito, Ryota, Iwashita, Kazuhiro, and Maruyama, Jun-ichi

Subjects
KOJI, NUCLEOTIDE sequence, GENES, CELL fusion, POTENTIAL functions, MYCOLOGY
Abstract

In the Pezizomycotina (filamentous ascomycete) species, genes that encode proteins with an HET domain (Pfam: PF06985) are reportedly involved in heterokaryon incompatibility (HI) in which cell death or growth defects are induced after fusion of cells that are genetically incompatible owing to diversities in their nucleotide sequence. HET domain genes are commonly found in Pezizomycotina genomes and are functionally characterized in only a few species. Here, we compared 44 HET domain genes between an incompatible strain pair of Aspergillus oryzae RIB40 and RIB128 and performed inter-strain expression of 37 sequence-diverse genes for mimicking HI. Four HET domain genes were identified to cause severe growth inhibition in a strain- or sequence-specific manner. Furthermore, SNPs responsible for the inhibition of cell growth were identified. This study provides an important insight into the physiological significance of sequence diversity of HET domain genes and their potential functions in HI of A. oryzae. Inter-strain expression of sequence-diverse HET domain genes severely inhibited growth in an incompatible strain pair of Aspergillus oryzae, and SNPs responsible for the growth inhibition were identified. [ABSTRACT FROM AUTHOR]

Published
2019
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