72 results on '"Iwahara Y"'
Search Results
2. Cold-sensitive ion channels are involved in chilling injury of pig oocytes
- Author
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Edashige, K., primary, Iwahara, Y., additional, Nariai, S., additional, Nishiya, Y., additional, Kitayama, M., additional, Niimi, S., additional, Seki, S., additional, Koshimoto, C., additional, Matsukawa, K., additional, and Kasai, M., additional
- Published
- 2016
- Full Text
- View/download PDF
3. Evaluation of minimal disseminated disease in cryopreserved ovarian tissue from bone and soft tissue sarcoma patients
- Author
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Dolmans, M.M., primary, Iwahara, Y., additional, Donnez, J., additional, Soares, M, additional, Vaerman, J.L., additional, Amorim, C.A., additional, and Poirel, H., additional
- Published
- 2016
- Full Text
- View/download PDF
4. Neutrophil depletion increase silica nanoparticle-induced placental cellular damage
- Author
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Higashisaka, K., primary, Iwahara, Y., additional, Yanagihara, I., additional, Saito, S., additional, Yoshioka, Y., additional, and Tsutsumi, Y., additional
- Published
- 2015
- Full Text
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5. ChemInform Abstract: Manganese-(II) and (III)-Mediated Free-Radical Cyclization of Alkenes, β-Keto Esters and Molecular Oxygen.
- Author
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YAMADA, T., primary, IWAHARA, Y., additional, NISHINO, H., additional, and KUROSAWA, K., additional
- Published
- 2010
- Full Text
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6. Immunoglobulin Prophylaxis against Milkborne Transmission of Human T Cell Leukemia Virus Type I in Rabbits
- Author
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Sawada, T., primary, Iwahara, Y., additional, Ishii, K., additional, Taguchi, H., additional, Hoshino, H., additional, and Miyoshi, I., additional
- Published
- 1991
- Full Text
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7. Transmission of HTLV-I by blood transfusion and its prevention by passive immunization in rabbits
- Author
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Kataoka, R, primary, Takehara, N, additional, Iwahara, Y, additional, Sawada, T, additional, Ohtsuki, Y, additional, Dawei, Y, additional, Hoshino, H, additional, and Miyoshi, I, additional
- Published
- 1990
- Full Text
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8. Transmission of HTLV‐I to rabbits via semen and breast milk from seropositive healthy persons
- Author
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Iwahara, Y., primary, Takehara, N., additional, Kataoka, R., additional, Sawada, T., additional, Ohtsuki, Y., additional, Nakachi, H., additional, Maehama, T., additional, Okayama, T., additional, and Miyoshi, I., additional
- Published
- 1990
- Full Text
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9. Effect of immunization on HTLV-I infection in rabbits.
- Author
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Takehara, N., Iwahara, Y., Uemura, Y., Sawada, T., Ohtsuki, Y., Iwai, H., Hoshino, H., and Miyoshi, I.
- Published
- 1989
- Full Text
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10. ChemInform Abstract: Manganese-(II) and (III)-Mediated Free-Radical Cyclization of Alkenes, β-Keto Esters and Molecular Oxygen.
- Author
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YAMADA, T., IWAHARA, Y., NISHINO, H., and KUROSAWA, K.
- Published
- 1993
- Full Text
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11. Isolation of HTLV-I from muscle of a patient with polymyositis.
- Author
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Ishii, K, Yamato, K, Iwahara, Y, Eguchi, T, Uemura, Y, Takehara, N, Ohtsuki, Y, Taguchi, H, and Miyoshi, I
- Subjects
- *
IMMUNOHISTOCHEMISTRY , *MYOSITIS , *RETROVIRUSES , *VIRAL antibodies - Published
- 1991
- Full Text
- View/download PDF
12. Factors related to a sense of security with medical and long-term care services among community-dwelling middle-aged and older adults in Japan.
- Author
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Fujita J, Fukui S, Fujikawa A, Iwahara Y, and Ishikawa T
- Subjects
- Aged, Cross-Sectional Studies, Female, Humans, Japan, Long-Term Care, Male, Middle Aged, Bereavement, Independent Living
- Abstract
Aim: The aim of this study was to identify factors related to a sense of security with regard to medical and long-term care services among middle-aged and older adults. These are for consideration of strategies for the establishment of a community-based integrated care system., Methods: A cross-sectional survey was conducted in 2400 men and women aged ≥40 years in two cities. Survey items included a scale for sense of security for medical and long-term care, experience of medical and long-term care services, and social and demographic factors. Data were analyzed using univariate and multiple regression analyses., Results: In total, 945 respondents were included in the analysis. In multiple regression analysis, sense of security was significantly higher with ability to consult with medical professionals on medical and long-term care, availability of emotional and instrumental support, norm of reciprocity in the community, community attachment, economic comfort, higher age and male gender, and significantly lower with experience of bereavement at a hospital and depression., Conclusions: These findings stress the importance of facilitating consultation with medical professionals, support for caregivers of terminally ill patients, and mutual support in the community in a community-based integrated care system. Geriatr Gerontol Int 2022; 22: 568-574., (© 2022 Japan Geriatrics Society.)
- Published
- 2022
- Full Text
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13. Massive eosinophilia despite severe aplastic anemia.
- Author
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Takahashi A, Iwahara Y, Machida H, Naruse K, Takeuchi E, and Shinohara T
- Abstract
Competing Interests: The authors declare that there is no conflict of interest.
- Published
- 2022
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14. Factors Related to End-of-Life Care Discussions Among Community-Dwelling People in Japan.
- Author
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Ishikawa T, Fukui S, Fujita J, Fujikawa A, Iwahara Y, and Takahashi K
- Subjects
- Adult, Cross-Sectional Studies, Death, Humans, Independent Living, Japan, Advance Care Planning, Terminal Care psychology
- Abstract
Context: End-of-life discussions regarding healthcare preferences are important to ensure a dignified end of life., Objectives: This study clarifies the factors associated with such discussions among community-dwelling people in Japan., Methods: We conducted a cross-sectional survey of 2400 randomly selected community-dwelling adults over the age of 40 in one urban and rural area each, in Japan. The survey investigated whether the respondents had engaged in discussions about their end-of-life care preferences. Logistic regression analyses were conducted to examine the physical, psychological, social, and demographic factors influencing end-of-life care discussions., Results: Of the final sample comprising 1172 respondents, 41.0% had engaged in end-of-life discussions. Of these, 95.2% had engaged in these discussions with family members, 11.0% with friends and acquaintances, and 4.4% with medical or long-term care personnel. Factors associated with end-of-life discussion engagement included requiring outpatient visits, long-term care requirements, availability of emotional support services, experience of the death of a relative within the past five years, living with a spouse, and being unemployed., Conclusion: The results showed that roughly 41% of the surveyed community-dwelling respondents had engaged in end-of-life discussions. The findings suggest that providing emotional support to relieve anxiety regarding one's health and the awareness of death, and having the opportunity to interact with others to discuss end-of-life issues, promote the engagement in such discussions in Japan. This study suggests that it is necessary to create an environment that provides social support and engenders awareness of the importance of end-of-life discussions while the community-dwelling people are still healthy., (Copyright © 2021 American Academy of Hospice and Palliative Medicine. Published by Elsevier Inc. All rights reserved.)
- Published
- 2022
- Full Text
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15. Measuring well-being in older adults: Identifying an appropriate single-item questionnaire.
- Author
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Fukui S, Ishikawa T, Iwahara Y, Fujikawa A, Fujita J, and Takahashi K
- Subjects
- Aged, Diagnostic Self Evaluation, Humans, Psychometrics, Surveys and Questionnaires, Happiness, Quality of Life
- Abstract
Aim: As the older adult population increases globally, it is important for local governments to measure their health and well-being consistently to effectively support this group. Because definitions of subjective well-being - such as happiness, life satisfaction and subjective health - overlap, it is worth considering which single-item indicator local governments can rely on to measure individual well-being. This study, therefore, explored the correlation between three single-item questionnaires on happiness, life satisfaction and subjective health used in government surveys, and established psychometric scales of happiness, life satisfaction and quality of life, and analyzed which of the three single-item indicators most effectively captured the target well-being., Methods: Randomly selected people aged ≥65 years in urban and rural areas (n = 1440) answered the questions on the Happiness Scale, the Satisfaction with Life Scale and the Quality of Life scale, and the three single-item questionnaires (happiness, life satisfaction and subjective health). We analyzed the data using correlation and multiple regression analyses., Results: The results showed a high positive correlation between the scales and the single-item questionnaires. The single-item life satisfaction and subjective health questionnaires were significantly correlated with all the three scales. Although there was not much difference in the adjusted R
2 values between putting three items jointly and separately, the value of single-item life satisfaction was the best and the subjective health was the second best., Conclusion: The results showed that the single-item life satisfaction and subjective health effectively captured the target well-being; these could be the appropriate indicators for a potential optimal index. Geriatr Gerontol Int 2021; 21: 1131-1137., (© 2021 Japan Geriatrics Society.)- Published
- 2021
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16. Seasonal variation in fish school spatial distribution and abundance under the Kuroshio regular pattern and the large meander in Suzu coastal waters.
- Author
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Zhu Y, Minami K, Iwahara Y, Oda K, Hidaka K, Hoson O, Morishita K, Hirota M, Tsuru S, Shirakawa H, and Miyashita K
- Subjects
- Animals, Population Dynamics, Biodiversity, Fisheries, Perciformes physiology, Seasons
- Abstract
The Kuroshio Current can take two paths; usually it follows the regular pattern but occasionally it follows a pattern known as the large meander. In this study, we investigated the abundance of fish that migrate to coastal waters and the spatial distribution of fish schools under both Kuroshio patterns in Suzu district, Kochi prefecture, where the set net is the main fishery industry. We clarified the seasonal variation in the density and distribution of fish schools using a quantitative echo sounder. The effects of the Kuroshio large meander (LM) depended on the season. There was no effect of current pattern in summer or autumn, but in winter and spring the LM altered the marine environment and fish distributions. Cold water masses were formed in the survey area during winter and spring during the LM, and the water temperature dropped significantly compared with during the Kuroshio non-large meander (NLM). This altered the fish species and the distribution of fish schools in the survey area. The catches of Japanese horse mackerels (Trachurus japonicus) and Yellowtails (Seriola quinqueradiata) were much higher during the LM compared with those during the NLM. Unlike these two species, the small-sized pelagic fishes in spring has decreased significantly during the LM., Competing Interests: The authors have declared that no competing interests exist.
- Published
- 2021
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17. Placenta accrete after a frozen-thawed embryo transfer in a systemic lupus erythematosus patient treated with hydroxychloroquine.
- Author
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Saito K, Mano C, Tatsumi T, Ishikawa T, Sekiguchi M, Iwahara Y, Hiramitsu S, and Miyasaka N
- Subjects
- Adult, Blastocyst, Cesarean Section, Cryopreservation, Female, Freezing, Humans, Infant, Newborn, Infertility, Female etiology, Infertility, Female therapy, Japan, Lupus Erythematosus, Systemic complications, Lupus Erythematosus, Systemic surgery, Placenta Accreta diagnosis, Placenta Accreta surgery, Pregnancy, Pregnancy Complications surgery, Pregnancy Outcome, Reproductive Techniques, Assisted, Treatment Outcome, Embryo Transfer adverse effects, Embryo Transfer methods, Hydroxychloroquine therapeutic use, Lupus Erythematosus, Systemic drug therapy, Placenta Accreta etiology, Pregnancy Complications drug therapy
- Abstract
Placenta accreta (PA) is a life-threatening disorder associated with decidual maldevelopment and a thin endometrium. Few cases of systemic lupus erythematosus (SLE) pregnancy complicated by PA have been reported, and the background pathophysiology remains elusive. Here, we report a case of PA in SLE pregnancy treated with hydroxychloroquine. A nulligravida woman with SLE, aged 41 years, visited our hospital because of infertility problems. Her SLE was treated with prednisolone and tacrolimus. We conducted assisted reproductive technology and gained several embryos. An artificial cycle successfully prepared the endometrium for embryo transfer with sufficient thickness. Over time, her SLE exacerbated, and we started hydroxychloroquine administration. Consequently, the endometrium did not respond to hormonal supplementation and remained thin, but we transferred the embryo and managed to achieve pregnancy. On the 38th week of gestation, we conducted labor induction because of elevated blood pressure. Induction was not effective, so we performed cesarean section; PA was observed. We performed compression suturing and were able to stop the hemorrhage. Postoperative uterine infarction and pelvic infection were successfully managed with conservative treatment. The present case highlights the use of hydroxychloroquine during endometrial development and contributes evidence regarding the pathogenesis of PA in pregnancy complicated by SLE.
- Published
- 2020
- Full Text
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18. Large leiomyomatosis peritonealis disseminata after laparoscopic myomectomy: A case report with literature review.
- Author
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Yoshino Y, Yoshiki N, Nakamura R, Iwahara Y, Ishikawa T, and Miyasaka N
- Abstract
Introduction: Leiomyomatosis peritonealis disseminata (LPD) is a rare disease in which multiple leiomyomas are formed intraperitoneally. Several LPD cases were associated with laparoscopic myomectomy using power morcellators; however, LPD with a large tumor size remains extremely rare. We present a case of large LPD occurring after laparoscopic surgery., Presentation of Case: A 26-year-old woman, gravida 0, underwent laparoscopic myomectomy with power morcellation in our institution. After 5 years, follow-up examination revealed pelvic tumors. Although we recommended resection, she refused and only wanted to be followed up. After 9 years from the first surgery, the tumors became symptomatic and were increasing in number (>10 nodules) and size (>15 cm). Needle biopsy detected leiomyoma. Computed tomography angiography showed that omental and mesenteric arteries were feeding the tumors. We performed laparotomy, and all the 19 tumors emerging from the omentum and mesenterium and weighing 7647 g in total were removed without injuring other organs. The maximum diameter of the largest tumor was 34 cm. The pathological diagnosis was nonmalignant LPD with leiomyoma., Discussion: Among all reported cases, our case had the largest LPD size. The tumors reached such a huge size because of two possible reasons: (1) they gradually grew asymptomatically over a long period from the time of diagnosis, and (2) they were fed by particularly large vessels, including the omental and mesenteric arteries., Conclusion: A large LPD is not always symptomatic. After a laparoscopic myomectomy, especially with power morcellation, long-term follow-up is necessary to detect LPD., (Copyright © 2020 The Author(s). Published by Elsevier Ltd.. All rights reserved.)
- Published
- 2020
- Full Text
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19. Pneumocystis jirovecii pneumonia in an HIV-infected patient mimicking acute eosinophilic pneumonia: a case report with a review of the literature.
- Author
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Ohji H, Shinohara T, Kadota N, Okano Y, Naruse K, Iwahara Y, and Ogushi F
- Abstract
Competing Interests: Conflicts of Interest: The authors have no conflicts of interest to declare.
- Published
- 2018
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20. Neutrophil Depletion Exacerbates Pregnancy Complications, Including Placental Damage, Induced by Silica Nanoparticles in Mice.
- Author
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Higashisaka K, Nakashima A, Iwahara Y, Aoki A, Nakayama M, Yanagihara I, Lin Y, Nagano K, Tsunoda SI, Saito S, Yoshioka Y, and Tsutsumi Y
- Subjects
- Animals, Apoptosis, Biomarkers, Disease Models, Animal, Female, Mice, Placenta blood supply, Pregnancy, Pregnancy Complications pathology, Metal Nanoparticles adverse effects, Neutrophils immunology, Neutrophils metabolism, Placenta metabolism, Placenta pathology, Pregnancy Complications etiology, Pregnancy Complications metabolism, Silicon Dioxide adverse effects
- Abstract
Recent advances in nanotechnology have led to the development of nanoparticles with innovative functions in various fields. However, the biological effects of nanoparticles-particularly those on the fetus-need to be investigated in detail, because several previous studies have shown that various nanoparticles induce pregnancy complications in mice. In this regard, our previous findings in mice suggested that the increase in peripheral neutrophil count induced by treatment with silica nanoparticles with a diameter of 70 nm (nSP70) may play a role in the associated pregnancy complications. Therefore, here, we sought to define the role of neutrophils in nSP70-induced pregnancy complications. The peripheral neutrophil count in pregnant BALB/c mice at 24 h after treatment with nSP70 was significantly higher than in saline-treated mice. In addition, maternal body weight, uterine weight, and the number of fetuses in nSP70-treated mice pretreated with anti-antibodies, which deplete neutrophils, were significantly lower than those in nSP70-treated mice pretreated with phosphate-buffered saline or isotype-matched control antibodies. Histology revealed that neutrophil depletion increased nSP70-induced placental damage from the decidua through the spongiotrophoblast layer and narrowed spiral arteries in the placentae. In addition, depletion of neutrophils augmented nSP70-induced cytotoxicity to fetal vessels, which were covered with endothelium. The rate of apoptotic cell death was significantly higher in the placentae of anti-nSP70-treated mice than in those from mice pretreated with isotype-matched control antibodies. Therefore, impairment of placental vessels and apoptotic cell death due to nSP70 exposure is exacerbated in the placentae of nSP70-treated mice pretreated with anti-antibodies. Depletion of neutrophils worsens nSP70-induced pregnancy complications in mice; this exacerbation was due to enhanced impairment of placental vessels and increased apoptotic cell death in maternal placentae. Our results provide basic information regarding the mechanism underlying silica-nanoparticle-induced pregnancy complications.
- Published
- 2018
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21. Hepatosplenic Hodgkin lymphoma without lymphadenopathy following reversible methotrexate-associated lymphoproliferative disorder.
- Author
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Tsukazaki Y, Shinohara T, Tanaka K, Naruse K, Iwahara Y, and Inoue S
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- Aged, 80 and over, Female, Humans, Infliximab therapeutic use, Liver pathology, Methotrexate therapeutic use, Spleen pathology, Arthritis, Rheumatoid drug therapy, Hodgkin Disease diagnosis, Immunosuppressive Agents therapeutic use, Lymphadenopathy diagnosis, Methotrexate adverse effects
- Abstract
Lymphoproliferative disorders (LPDs) occur more frequently in rheumatoid arthritis (RA) patients treated with immunosuppressive agents than in the non-RA population. However, the various forms of disease progression have not yet been elucidated in detail. We encountered a case of Epstein-Barr virus (EBV)-positive atypical polymorphous LPD in the cervical and intraabdominal lymph nodes with hepatosplenomegaly in an 88-year-old female with RA who had taken infliximab and methotrexate (MTX) for six years. Although spontaneous remission occurred following the withdrawal of infliximab and MTX, reversible LPD evolved into hepatosplenic Hodgkin lymphoma without lymphadenopathy presenting as a cholestatic febrile illness. Our findings suggest that the recurrent lesions of MTX-associated LPDs may not always coincide with the primary lesion and may present unexplained findings based on various extranodal diseases.
- Published
- 2017
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22. Non-Hodgkin's lymphoma involving a femur bone and bilateral adrenal glands alone with adrenal insufficiency.
- Author
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Iwahara Y, Shinohara T, Naruse K, and Komatsu Y
- Subjects
- Adrenal Gland Neoplasms drug therapy, Adrenal Gland Neoplasms pathology, Antibodies, Monoclonal, Murine-Derived therapeutic use, Antineoplastic Combined Chemotherapy Protocols therapeutic use, Cyclophosphamide therapeutic use, Doxorubicin therapeutic use, Femoral Neoplasms drug therapy, Femoral Neoplasms pathology, Humans, Lymphoma, Large B-Cell, Diffuse drug therapy, Lymphoma, Large B-Cell, Diffuse pathology, Magnetic Resonance Imaging, Male, Middle Aged, Neoplasms, Multiple Primary drug therapy, Neoplasms, Multiple Primary pathology, Prednisone therapeutic use, Rituximab, Tomography, X-Ray Computed, Vincristine therapeutic use, Adrenal Gland Neoplasms diagnostic imaging, Femoral Neoplasms diagnostic imaging, Lymphoma, Large B-Cell, Diffuse diagnostic imaging, Neoplasms, Multiple Primary diagnostic imaging
- Abstract
Primary bone lymphoma and primary adrenal lymphoma are rare clinicopathological entities of non-Hodgkin's lymphoma (NHL). We present the first case of diffuse large B-cell lymphoma with the involvement of a single bone and both adrenal glands alone with adrenal insufficiency. As primary extranodal NHL may have other unusual extranodal lesions, which may present unexplained clinical findings, patients with primary extranodal NHL require careful systemic examination, even when lymphadenopathy is absent., Competing Interests: Competing interests: None declared., (2017 BMJ Publishing Group Ltd.)
- Published
- 2017
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23. Disseminated tuberculosis after pregnancy progressed to paradoxical response to the treatment: report of two cases.
- Author
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Shinohara T, Kagawa K, Okano Y, Sawada T, Kobayashi T, Takikawa M, Iwahara Y, and Ogushi F
- Subjects
- Acute Disease, Adult, Disease Progression, Female, Humans, Peritonitis, Tuberculous diagnostic imaging, Peritonitis, Tuberculous immunology, Postpartum Period immunology, Pregnancy, Psoas Abscess diagnostic imaging, Psoas Abscess etiology, Psoas Abscess immunology, Puerperal Infection diagnostic imaging, Puerperal Infection immunology, Radiography, Thoracic, Tomography, X-Ray Computed, Treatment Outcome, Tuberculosis, Pulmonary diagnostic imaging, Tuberculosis, Pulmonary immunology, Tuberculosis, Spinal complications, Tuberculosis, Spinal diagnostic imaging, Tuberculosis, Spinal immunology, Antitubercular Agents therapeutic use, Peritonitis, Tuberculous drug therapy, Psoas Abscess drug therapy, Puerperal Infection drug therapy, Tuberculosis, Pulmonary drug therapy, Tuberculosis, Spinal drug therapy
- Abstract
Background: Early postpartum women are more likely to develop tuberculosis than nonpregnant women mainly due to immune reconstitution after delivery. Paradoxical response (PR) during antituberculosis treatment also arises via recovery from immunosuppression. However, no study focused on PR during antituberculosis treatment in a postpartum patient has been reported., Case Presentation: We present two sequential cases (Patient 1: 26-year-old; Patient 2: 29-year-old) of postpartum tuberculosis with pulmonary and extrapulmonary lesions (Patient 1: peritonitis; Patient 2: psoas abscess secondary to spondylitis). Both cases progressed to PR (worsening of pre-existing lung infiltrations (Patients 1, 2) and new contralateral effusion (Patient 2)) in a relatively short time after initiation of treatment (Patient 1: 1 week; Patient 2: 3 weeks), suggesting that immune modulations during pregnancy and delivery may contribute to the pathogenesis of both disseminated tuberculosis and its PR. The pulmonary lesions and effusion of both cases gradually improved without change of chemotherapy regimen., Conclusion: Physicians should recognize PR in tuberculosis patients with postpartum and then evaluate treatment efficacy.
- Published
- 2016
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24. Pyothorax-associated lymphoma (PAL) with biclonal Epstein-Barr virus infection: characterization of a novel PAL cell line with unique features.
- Author
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Taniguchi A, Hashida Y, Nemoto Y, Taguchi T, Iwahara Y, and Daibata M
- Subjects
- Aged, Animals, Blotting, Southern, Cytogenetic Analysis, Empyema, Tuberculous genetics, Epstein-Barr Virus Infections complications, Epstein-Barr Virus Infections genetics, Female, Herpesvirus 4, Human genetics, Humans, Immunophenotyping, In Situ Hybridization, Fluorescence, Lymphoma pathology, Lymphoma, Large B-Cell, Diffuse pathology, Male, Mice, Mice, Inbred BALB C, Mice, Nude, Middle Aged, Mycobacterium tuberculosis pathogenicity, Phenotype, Tumor Cells, Cultured, Empyema, Tuberculous virology, Epstein-Barr Virus Infections virology, Genome, Viral, Herpesvirus 4, Human pathogenicity, Lymphoma etiology, Lymphoma, Large B-Cell, Diffuse etiology
- Abstract
Pyothorax-associated lymphoma (PAL) is a representative form of diffuse large B-cell lymphoma associated with chronic inflammation, in which the Epstein-Barr virus (EBV) genome is consistently detectable in the lymphoma cells of all PAL cases. Cell lines and animal models would be useful for understanding better this rare lymphoma, but reports of PAL-derived cell lines are scarce. We report a new PAL cell line, designated Pal-2, with unique phenotypic expression. Pal-2 is the first PAL cell line that carries a biclonal EBV infection with abundant viral genome and that exhibits tumorigenic capacity once injected into nude mice., (Copyright © 2013 Elsevier Ltd. All rights reserved.)
- Published
- 2013
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25. Liver-specific microRNAs as biomarkers of nanomaterial-induced liver damage.
- Author
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Nagano T, Higashisaka K, Kunieda A, Iwahara Y, Tanaka K, Nagano K, Abe Y, Kamada H, Tsunoda S, Nabeshi H, Yoshikawa T, Yoshioka Y, and Tsutsumi Y
- Subjects
- Animals, Female, Liver drug effects, Mice, Mice, Inbred BALB C, Particle Size, Sensitivity and Specificity, Silicon Dioxide toxicity, Biomarkers blood, Chemical and Drug Induced Liver Injury blood, MicroRNAs blood, Nanoparticles toxicity
- Abstract
Although nanomaterials are being used in various fields, their safety is not yet sufficiently understood. We have been attempting to establish a nanomaterials safety-assessment system by using biomarkers to predict nanomaterial-induced adverse biological effects. Here, we focused on microRNAs (miRNAs) because of their tissue-specific expression and high degree of stability in the blood. We previously showed that high intravenous doses of silica nanoparticles of 70 nm diameter (nSP70) induced liver damage in mice. In this study, we compared the effectiveness of serum levels of liver-specific or -enriched miRNAs (miR-122, miR-192, and miR-194) with that of conventional hepatic biomarkers (alanine aminotransferase (ALT) and aspartate aminotransferase (AST)) as biomarkers for nSP70. After mice had been treated with nSP70, their serum miRNAs levels were measured by using quantitative RT-PCR. Serum levels of miR-122 in nSP70-treated mice were the highest among the three miRNAs. The sensitivity of miR-122 for liver damage was at least as good as those of ALT and AST. Like ALT and AST, miR-122 may be a useful biomarker of nSP70. We believe that these findings will help in the establishment of a nanomaterials safety-assessment system.
- Published
- 2013
- Full Text
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26. Video-assisted thoracic surgery lobectomy for lung cancer in a patient with complete situs inversus.
- Author
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Yoshida M, Hino H, Machida H, Hatakeyama N, Okano Y, Iwahara Y, Shinohara T, and Oogushi F
- Subjects
- Aged, Humans, Lung diagnostic imaging, Lung surgery, Male, Radiography, Situs Inversus diagnostic imaging, Lung Neoplasms surgery, Pneumonectomy methods, Thoracic Surgery, Video-Assisted methods
- Abstract
Complete situs inversus is a rare abnormality of autosomal recessive inheritance; it requires particular care during surgery, because the viscus anatomy is a mirror image of the normal anatomy. Reports of surgery for lung cancer in cases of complete situs inversus are very rare. Here, we report a case of lobectomy for lung cancer of the right lower lobe performed using video-assisted thoracic surgery (VATS) in a patient with complete situs inversus. We emphasize the importance of careful examination of the relationship between the bronchus, pulmonary artery, and pulmonary vein in the hilum of the lung in cases of complete situs inversus requiring lung resection for cancer; this is even more necessary when VATS is performed.
- Published
- 2013
- Full Text
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27. Augmentation of arginase Ⅱ expression in the human endometrial epithelium in the secretory phase.
- Author
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Tajima M, Harada T, Ishikawa T, Iwahara Y, and Kubota T
- Subjects
- Blotting, Western, Epithelial Cells enzymology, Epithelium enzymology, Female, Follicular Phase metabolism, Humans, Immunohistochemistry, Isoenzymes analysis, Polyamines analysis, Proline analysis, Reverse Transcriptase Polymerase Chain Reaction, Arginase analysis, Endometrium enzymology, Luteal Phase metabolism
- Abstract
L-arginine is the common substrate for arginase and nitric oxide synthase (NOS). Arginase converts L-arginine to urea and L-ornithine. L-Ornithine is the principal precursor for the production of polyamines and L-proline, which are required for cell proliferation and collagen synthesis. Endothelial NOS is expressed in the human endometrial glandular epithelium, but the expression and physiological roles of arginase in the human endometrium are not clear. The objective of this study was to investigate the expression and distribution patterns of arginases Ⅰ (A-Ⅰ) and Ⅱ (A-Ⅱ) in the human endometrium by using immunohistochemistry, reverse transcription-polymerase chain reaction (RTPCR), and western blotting. A-Ⅰ and A-Ⅱ were detected by immunohistochemistry in human endometrial epithelial cells during the proliferative and secretory phases of the menstrual cycle. RT-PCR showed that A-Ⅰ and A-Ⅱ mRNA were expressed in human endometrial tissue. Western blotting analysis results showed the expression of A-Ⅱ protein. Immunohistochemistry and western blotting results showed that expression levels of A-Ⅱ were significantly higher in the secretory phase than in the proliferative phase. Increased A-Ⅱ levels in the secretory phase may be responsible for endometrial growth by increasing polyamines and proline products.
- Published
- 2012
28. Expression of heme oxygenase in the eutopic and ectopic endometrium in patients with adenomyosis.
- Author
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Iwahara Y, Nagai A, Yoshiki N, Igarashi K, Yamashita K, and Kubota T
- Subjects
- Apoptosis, Female, Humans, Menstrual Cycle metabolism, Oxidative Stress, Adenomyosis enzymology, Endometrium enzymology, Heme Oxygenase (Decyclizing) metabolism, Heme Oxygenase-1 metabolism
- Abstract
Heme oxygenase (HO) is the rate-limiting enzyme that catalyzes the degradation of heme into iron, carbon monoxide, and biliverdin. This enzyme has important functions in cellular homeostasis, including the regulation of oxidative load, apoptosis, and inflammation. Two isoforms of HO, the inducible HO-1 and the constitutive HO-2, are expressed and are known to play a role in the normal human endometrium throughout the menstrual cycle, but there is little evidence for HO expression and behavior in adenomyosis, which is the occurrence of intramural ectopic endometrial tissue. The aim of this study was to investigate the presence and localization of the two HO isoforms in both eutopic and ectopic endometrium of women with adenomyosis during the menstrual cycle. The oxidative stress and apoptosis related to HO-1 expression were also assessed. The expression of HO-1 and HO-2 in both eutopic and ectopic endometrium was confirmed, and their levels in the ectopic endometrium were lower than those in the eutopic endometrium. The cyclic variability of HO expression was lost in the ectopic endometrium during the menstrual cycle, whereas this variability was apparent in the eutopic endometrium. Moreover, HO-1 expression corresponded to apoptotic events in the eutopic endometrium. Constitutive HO-2 expression corresponded to endometrial proliferation and degradation. These results reveal that both HO-1 and HO-2 contribute little in the pathophysiology of adenomyosis.
- Published
- 2012
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29. Cancer of the left lung with persistent left superior vena cava.
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Yoshida M, Hino H, Machida H, Hatakeyama N, Okano Y, Iwahara Y, Shinohara T, and Oogushi F
- Subjects
- Adenocarcinoma diagnosis, Adenocarcinoma surgery, Adenocarcinoma of Lung, Aged, 80 and over, Biopsy, Bronchoscopy, Diagnosis, Differential, Female, Follow-Up Studies, Humans, Imaging, Three-Dimensional, Lung Neoplasms diagnosis, Lung Neoplasms surgery, Pneumonectomy, Positron-Emission Tomography, Tomography, X-Ray Computed, Vascular Malformations diagnosis, Vascular Malformations surgery, Vascular Surgical Procedures methods, Adenocarcinoma complications, Lung Neoplasms complications, Vascular Malformations complications, Vena Cava, Superior abnormalities
- Abstract
As operations for lung cancer become more common, more anomalies of various pulmonary arteries and veins are being encountered. Persistent left superior vena cava (PLSVC) is caused by abnormal development of the sinus venosus in early fetal life. In cases of PLSVC with left lung cancer, particular care must be exercised during the excision of the pulmonary vein, focusing on the region into which the PLSVC flows. In such anomalous cases, proper excision of the pulmonary artery and vein and lymph node dissection require a firm grasp of anatomic detail using preoperative computed tomographic scanning., (Copyright © 2012 The Society of Thoracic Surgeons. Published by Elsevier Inc. All rights reserved.)
- Published
- 2012
- Full Text
- View/download PDF
30. [Rib-originated fibrous dysplasia: report of a case].
- Author
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Yoshida M, Hino H, Abe S, Machida H, Hatakeyama N, Okano Y, Iwahara Y, Shinohara T, and Oogushi F
- Subjects
- Fibrous Dysplasia of Bone pathology, Humans, Male, Middle Aged, Fibrous Dysplasia of Bone surgery, Ribs
- Abstract
The incidence of fibrous dysplasia (FD) is not frequent in the case of benign bone tumors of the chest wall, and differential diagnosis between FD and the malignancy on the basis of imaging findings is difficult. We report a case of a painful FD lesion (size, 9×8 cm) that originated from the 5th rib of a 52-year-old man and was surgically resected. His symptoms improved after the operation. Painful and large FD lesions should be resected because of a difficulty in differential diagnosis from malignant tumors.
- Published
- 2012
31. CD5-positive diffuse large B cell lymphoma infiltrating the central nervous system presenting Guillain-Barré-like syndrome after chemotherapy.
- Author
-
Machida H, Shinohara T, Hatakeyama N, Okano Y, Nakano M, Tobiume M, Naruse K, Iwahara Y, and Ogushi F
- Subjects
- Aged, 80 and over, Antigens, Neoplasm genetics, Antigens, Neoplasm immunology, CD5 Antigens genetics, Cell Movement, Central Nervous System drug effects, Central Nervous System immunology, Cytarabine administration & dosage, Female, Guillain-Barre Syndrome drug therapy, Guillain-Barre Syndrome etiology, Guillain-Barre Syndrome immunology, Guillain-Barre Syndrome pathology, Humans, Immunoglobulins, Intravenous adverse effects, Lymphoma, Large B-Cell, Diffuse cerebrospinal fluid, Lymphoma, Large B-Cell, Diffuse drug therapy, Lymphoma, Large B-Cell, Diffuse immunology, Methotrexate administration & dosage, Prednisolone administration & dosage, Steroids adverse effects, Antineoplastic Combined Chemotherapy Protocols, CD5 Antigens immunology, Central Nervous System pathology, Lymphoma, Large B-Cell, Diffuse pathology
- Abstract
An 83-year-old woman was admitted to our hospital with abdominal pain. Examination revealed mediastinal lymphoadenopathy, hepatosplenomegaly, and infiltration of abnormal cells into the bone marrow with hemophagocytosis, and CD5-positive diffuse large B cell lymphoma was diagnosed. Chemotherapy was administered and progressive weakness of the limbs, resembling a Guillain-Barré-like syndrome, subsequently appeared. Cerebrospinal fluid examination indicated lymphoma cell infiltration. Although immune globulin and steroid therapies were not effective, intrathecal injection of methotrexate, predonisolone, and cytarabine improved these symptoms. Subsequent to chemotherapy, cell surface antigen changes were observed in the cerebrospinal fluid relative to those in bone marrow.
- Published
- 2012
- Full Text
- View/download PDF
32. [Exacerbation of cranial nerurological symptoms by platelet transfusion before the diagnosis of thrombotic thrombocytopenic purpura].
- Author
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Machida H, Shinohara T, Hatakeyama N, Okano Y, Nakano M, Tobiume M, Iwahara Y, and Ogushi F
- Subjects
- ADAM Proteins blood, ADAMTS13 Protein, Anemia, Hemolytic, Autoimmune, Biomarkers, Diagnosis, Differential, Disease Progression, Female, Humans, Middle Aged, Purpura, Thrombotic Thrombocytopenic complications, Purpura, Thrombotic Thrombocytopenic diagnosis, Thrombocytopenia, Cranial Nerve Diseases etiology, Platelet Transfusion adverse effects, Purpura, Thrombotic Thrombocytopenic therapy
- Abstract
A 47-year-old woman was transported to our hospital because of vomiting and syncope after breakfast. Physical examination revealed icterus and anemia of bulbar conjunctivas, and abnormal neurological findings were detected. Laboratory data indicated marked anemia and thrombocytopenia (Hb 5.2 g/dl, Plt. 0.6×10(4)/μl), but no leukocyte abnormalities were found. Transaminase was slightly elevated, and serum indirect bilirubin in was also elevated. Based on these data, we initially suspected Evan's syndrome, which involves idiopathic thrombocytopenic purpura with autoimmune hemolytic anemia. So we transfused red blood cells, performed platelet transfusion, and administered steroids, but there was no response to these therapies. On the 4th day of admission, she developed a stroke followed by coma. After the stroke, we diagnosed the case as thrombotic thrombocytopenic purpura (TTP) because laboratory findings showed diminished activity of ADAMTS 13 (a disintegrin-like metalloproteinase with thrombospondin type 1 motifs 13) and ADAMTS 13 antigen. It is important to suspect TTP when hemolytic anemia with thrombocytopenia is observed, and to check the activity and antigen of ADAMTS13 immediately for the diagnosis. Platelet transfusion should be done cautiously in these cases.
- Published
- 2012
33. [A case of pulmonary nontuberculous mycobacteriosis induced by tocilizumab treatment for rheumatoid arthritis].
- Author
-
Inayama M, Shinohara T, Hosokawa E, Machida H, Iwahara Y, and Ogushi F
- Subjects
- Aged, Female, Humans, Antibodies, Monoclonal, Humanized adverse effects, Arthritis, Rheumatoid drug therapy, Lung Diseases etiology, Mycobacterium avium-intracellulare Infection etiology
- Abstract
A 65-year-old woman whose rheumatoid arthritis was treated with tocilizumab (TCZ) was found in chest radiography to have a new consolidation in the right lower lung field. Positive Mycobacterium intracellulare and Mycobacterium avium cultures in sputum and bronchial secretions yielded a diagnosis of pulmonary nontuberculous mycobacteriosis. The most common adverse TCZ effect is infection. This case highlights the fact that those treated with TCZ should be considered at elevated risk for developing nontuberculous mycobacteriosis.
- Published
- 2011
- Full Text
- View/download PDF
34. Acute myeloid leukemia with myelodysplasia-related changes mimicking acute promyelocytic leukemia.
- Author
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Machida H, Shinohara T, Iwahara Y, Hatakeyama N, Okano Y, Nakano M, Hosokawa E, Abe S, Naruse K, and Ogushi F
- Subjects
- Aged, Antineoplastic Agents therapeutic use, Chromosome Deletion, Chromosomes, Human, Pair 3 genetics, Diagnosis, Differential, Humans, Leukemia, Myeloid, Acute drug therapy, Leukemia, Myeloid, Acute genetics, Male, Oncogene Proteins, Fusion genetics, Tretinoin therapeutic use, Leukemia, Myeloid, Acute diagnosis, Leukemia, Promyelocytic, Acute diagnosis, Myelodysplastic Syndromes diagnosis
- Abstract
We describe a patient with acute myeloid leukemia with myelodysplasia-related changes (AML-MRC) that clinically resembled acute promyelocytic leukemia (APL). The karyotype of his leukemic cells was 46, XY, del (3) (q?) and did not include a chromosomal translocation involving the retinoic acid receptor-α gene. However, retinoic acid syndrome developed, and partial remission was achieved after treatment with all-trans retinoic acid (ATRA) followed by chemotherapy. Our case might provide new insights into the mechanism of the growth inhibitory effect of ATRA on APL-like cells.
- Published
- 2011
- Full Text
- View/download PDF
35. Immunoglobulin D-lambda type multiple myeloma presenting with FDG-PET/CT negative bone marrow involvement.
- Author
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Machida H, Shinohara T, Hino H, Yoshida M, Hatakeyama N, Okano Y, Inayama M, Hosokawa E, Abe S, Naruse K, Iwahara Y, and Ogushi F
- Subjects
- Aged, Bone Marrow diagnostic imaging, Bone Marrow pathology, Diagnosis, Differential, Fluorodeoxyglucose F18, Humans, Magnetic Resonance Imaging, Male, Monoclonal Gammopathy of Undetermined Significance diagnosis, Multiple Myeloma diagnosis, Plasmacytoma diagnosis, Plasmacytoma diagnostic imaging, Plasmacytoma immunology, Positron-Emission Tomography, Radiopharmaceuticals, Tomography, X-Ray Computed, Immunoglobulin D metabolism, Multiple Myeloma diagnostic imaging, Multiple Myeloma immunology
- Abstract
We here report a rare case of a patient with IgD-lambda-positive multiple myeloma presenting with FDG-PET/CT negative bone marrow involvement. A 72-year-old man was admitted to our hospital for evaluation of a paravertebral tumor of the chest. Thoracotomy was performed and a histopathological evaluation of resected intrathoracic tumor demonstrated a plasmacytic neoplasma. Initially we thought that this case was a solitary plasmacytoma because there were no positive findings on postoperative FDG-PET/CT. However, bone marrow aspiration study demonstrated massive infiltration of myeloma cells (72%). It is necessary to recognize that IgD-lambda type myeloma cells may not be sufficiently metabolically active to form high uptake on FDG-PET/CT.
- Published
- 2011
- Full Text
- View/download PDF
36. Autopsy case of rapid progressive atypical carcinoid of the lung discovered with multiple nodular shadows.
- Author
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Okano Y, Inayama M, Hatakeyama N, Hino H, Iwahara Y, Motoki T, Naruse K, Yokota Y, and Ogushi F
- Subjects
- Aged, 80 and over, Antineoplastic Combined Chemotherapy Protocols therapeutic use, Autopsy, Bone Neoplasms secondary, Carcinoid Tumor therapy, Disease Progression, Fatal Outcome, Humans, Liver Neoplasms secondary, Lung Neoplasms therapy, Male, Thoracic Surgery, Video-Assisted, Time Factors, Tomography, X-Ray Computed, Carcinoid Tumor diagnostic imaging, Carcinoid Tumor pathology, Lung diagnostic imaging, Lung Neoplasms diagnostic imaging, Lung Neoplasms pathology
- Abstract
We report an autopsy case of rapid progressive atypical carcinoid of the lung discovered as multiple nodular shadows. The patient was an 82-year-old man with non-productive cough. Both chest radiography and computed tomography revealed multiple nodules in both the lung and pleural effusion. Multiple metastases to bone and liver were also noted. Samples from the wall-side pleural lesion were obtained by video-assisted thoracoscopic surgery under local anesthesia, and histological examination led to a diagnosis of atypical carcinoid. Treatment was ineffective and the patient died on day 39 after admission. The primary site was identified as the lung after autopsy. We believe that early detection is crucial in the treatment of atypical carcinoid due to poor prognosis.
- Published
- 2008
- Full Text
- View/download PDF
37. [A case of adult onset idiopathic pulmonary hemosiderosis markedly improved by steroid therapy].
- Author
-
Inayama M, Hino H, Takezaki A, Otsuka S, Machida H, Hatakeyam N, Iwahara Y, Motoki T, Ohtsuki Y, and Fumitaka O
- Subjects
- Biopsy, Hemosiderosis pathology, Humans, Lung Diseases pathology, Male, Middle Aged, Glucocorticoids therapeutic use, Hemosiderosis drug therapy, Lung Diseases drug therapy, Prednisolone therapeutic use
- Abstract
A case of adult onset idiopathic pulmonary haemosiderosis (IPH) was reported. A 53-year-old man was admitted to our hospital because of repeated bloody sputum on June 2, 2006. Chest radiograph on admission disclosed diffuse infiltrative shadows in both lung fields, and one month later these shadows became more marked. The chest CT on July 5, 2006 showed patchy areas of ground-glass opacity and consolidation, exhibiting a distinctly peripheral distribution. Bronchoscopic findings revealed oozing bleeding from the orifice of B5 in the right lung and B9 in the left lung. We employed video-assisted thoracoscopic surgery for lung biopsy and he as primary IPH was diagnosed clinicopathologically. His symptoms and radiographic findings were markedly improved after steroid therapy, followed by no signs of recurrence. It may be important to establish a definitive diagnosis early, even in IPH, using VATS, for further effective therapy.
- Published
- 2007
38. Adnexal torsion of mature cystic teratoma with hemorrhagic infarction: helpful findings on successive plain abdominal radiographs.
- Author
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Morita S, Takada Y, Katoh A, Iwahara Y, Kohyama A, Kuwae C, and Kita M
- Subjects
- Adnexal Diseases surgery, Female, Hemorrhage diagnostic imaging, Hemorrhage surgery, Humans, Middle Aged, Ovarian Neoplasms surgery, Teratoma surgery, Tomography, X-Ray Computed, Torsion Abnormality surgery, Adnexal Diseases diagnostic imaging, Ovarian Neoplasms diagnostic imaging, Teratoma diagnostic imaging, Torsion Abnormality diagnostic imaging
- Abstract
Adnexal torsion is a relatively rare cause of lower abdominal pain in women, but in many cases it leads to a serious condition. We present a case of adnexal torsion of a mature cystic teratoma in which hemorrhagic infarction was reflected by progressive enlargement and wall thickening on successive plain abdominal radiographs. There has been no other report describing such changes of adnexal torsion on plain abdominal radiographs.
- Published
- 2007
- Full Text
- View/download PDF
39. [Transient worsening of chest radiograph and development of lymphadenopathy during chemotherapy for miliary tuberculosis].
- Author
-
Iwahara Y, Motoki T, and Ogushi F
- Subjects
- Adult, Antitubercular Agents therapeutic use, Female, Humans, Radiography, Thoracic, Tuberculosis, Lymph Node etiology, Tuberculosis, Miliary diagnostic imaging, Tuberculosis, Miliary drug therapy
- Abstract
A-37-year old woman was referred to our hospital because of bilateral pulmonary micronodular shadows on chest X-ray. Prednisolone was reported to be administered for her coughs and dyspnea more than a month, but was discontinued recently. Under the diagnosis as miliary tuberculosis, we started to treat her with the combined use of pyrazinamide, isoniazid, rifampicin, and ethambutol. Then her symptoms subsided gradually. Two months later, however, high fever developed, followed by exacerbation of the radiographic shadows, and marked cervical and mediastinal lymphadenopathy. We considered them so-called paradoxical worsening, and continued the antituberculosis therapy unchanged. Those clinical manifestations began to subside about 4 months after the initiation of the treatment. Paradoxical worsening has been described as a relatively rare manifestation, and seem to be attributable to prompt recovery of the immunity to mycobacterial antigens after the use of antituberculous therapy. We considered that, in this case, disseminated tuberculosis and firstly administered steroid that might suppress immune function, and discontinuation of steroid therapy followed by the bactericidal antituberculous chemotherapy were associated with the development of the paradoxical reactions, by analogy with immune reconstitution syndrome frequently reported in HIV-related tuberculosis patients.
- Published
- 2006
40. [Case of multi-drug resistant tuberculosis complicated with bronchoesophageal fistula].
- Author
-
Hatakeyama N, Okano Y, Miki M, Iwahara Y, Nakamura Y, Motoki T, and Ogushi F
- Subjects
- Aged, Bronchial Fistula drug therapy, Esophageal Fistula drug therapy, Humans, Male, Treatment Outcome, Tuberculosis, Multidrug-Resistant diagnosis, Tuberculosis, Pulmonary diagnosis, Antitubercular Agents administration & dosage, Bronchial Fistula etiology, Esophageal Fistula etiology, Tuberculosis, Multidrug-Resistant complications, Tuberculosis, Multidrug-Resistant drug therapy, Tuberculosis, Pulmonary complications, Tuberculosis, Pulmonary drug therapy
- Abstract
A 71-year-old man visited the surgical department of our hospital due to back pain in November, 1999. Esophagogram showed bronchoesophageal fistula and a plain chest radiograph showed a nodular shadow in the left upper area with left pleural effusion. As the symptom disappeared on treatment with an antimicrobial agent, he was followed up as an outpatient. In August, 2001, he presented to the Respiratory Department of our hospital with low-grade fever and productive cough. Since tubercle bacilli were detected in a sputum culture, combined treatment with antituberculous chemotherapy was started. But, none of the symptoms were improved. These bacilli were identified as being multi-drug-resistant. After the chemotherapy regimen was changed, the symptoms disappeared and the esophagorespiratory fistula was closed. We report a rare case of esophagorespiratory fistula with multi-drug resistant tuberculosis, which was improved by chemotherapy.
- Published
- 2004
41. Copper deficiency anaemia.
- Author
-
Miyoshi I, Saito T, and Iwahara Y
- Subjects
- Adult, Anemia, Hypochromic pathology, Humans, Male, Anemia, Hypochromic etiology, Copper deficiency
- Published
- 2004
- Full Text
- View/download PDF
42. Isonitrile hydratase from Pseudomonas putida N19-2. Cloning, sequencing, gene expression, and identification of its active acid residue.
- Author
-
Goda M, Hashimoto Y, Takase M, Herai S, Iwahara Y, Higashibata H, and Kobayashi M
- Subjects
- Amino Acid Sequence, Base Sequence, Cloning, Molecular, DNA, Bacterial, Electrophoresis, Polyacrylamide Gel, Hydro-Lyases chemistry, Hydro-Lyases isolation & purification, Hydro-Lyases metabolism, Molecular Sequence Data, Plasmids, Recombinant Proteins chemistry, Recombinant Proteins genetics, Recombinant Proteins isolation & purification, Recombinant Proteins metabolism, Sequence Homology, Amino Acid, Hydro-Lyases genetics, Pseudomonas putida enzymology
- Abstract
Isonitrile hydratase is a novel enzyme in Pseudomonas putida N19-2 that catalyzes the conversion of isonitriles to N-substituted formamides. Based on N-terminal and internal amino acid sequences, a 535-bp DNA fragment corresponding to a portion of the isonitrile hydratase gene was amplified, which was used as a probe to clone a 6.4-kb DNA fragment containing the whole gene. Sequence analysis of the 6.4-kb fragment revealed that the isonitrile hydratase gene (inhA) was 684 nucleotides long and encoded a protein with a molecular mass of 24,211 Da. Overexpression of inhA in Escherichia coli gave a large amount of soluble isonitrile hydratase exhibiting the same molecular and catalytic properties as the native enzyme from the Pseudomonas strain. The predicted amino acid sequence of inhA showed low similarity to that of an intracellular protease in Pyrococcus horikoshii (PH1704), and an active cysteine residue in the protease was conserved in the isonitrile hydratase at the corresponding position (Cys-101). A mutant enzyme containing Ala instead of Cys-101 did not exhibit isonitrile hydratase activity at all, demonstrating the essential role of this residue in the catalytic function.
- Published
- 2002
- Full Text
- View/download PDF
43. [A case of Legionella pneumonia successfully treated intravenously with both erythromycin and ciprofloxacin].
- Author
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Okano Y, Motoki T, Miki M, Hatakeyama N, Iwahara Y, Nakamura Y, and Ogushi F
- Subjects
- Humans, Hyponatremia complications, Injections, Intravenous, Legionnaires' Disease complications, Male, Middle Aged, Anti-Infective Agents administration & dosage, Ciprofloxacin administration & dosage, Legionnaires' Disease drug therapy
- Abstract
A 54-year-old man was admitted to our hospital complaining of a high fever and watery diarrhea. The chest radiograph on admission revealed a homogeneous consolidation of the left upper lobe. Laboratory findings included proteinuria, oligouria, hematuria, myoglobinuria, hyponatremia, and serum CPK elevation. On the basis of these findings, a tentative diagnosis of Legionella pneumonia was made. He was treated with sulbactam/cefoperazon and erythromycin, but his high fever remained and the consolidation shadow deteriorated. He was therefore given both erythromycin and ciprofloxacin intravenously. After several days the fever had returned to normal, the appearance of the chest radiograph had improved, and his symptoms were quickly relieved. This case suggests that intravenous administration of ciprofloxacin and erythromycin can be an effective treatment against Legionella pneumonia.
- Published
- 2001
44. Polymerase chain reaction amplification of Asp f I and alkaline protease genes from fungus balls: clinical application in pulmonary aspergillosis.
- Author
-
Urata T, Kobayashi M, Imamura J, Tanaka Y, Muneishi H, Iwahara Y, Uemura Y, Taguchi H, and Miyoshi I
- Subjects
- Adult, Aged, Antigens, Fungal genetics, Antigens, Plant, Aspergillosis diagnosis, Aspergillus fumigatus enzymology, Aspergillus fumigatus pathogenicity, Base Sequence, DNA Primers genetics, DNA, Fungal genetics, DNA, Fungal isolation & purification, Female, Fungal Proteins genetics, Humans, Lung Diseases, Fungal diagnosis, Male, Middle Aged, Polymerase Chain Reaction methods, Polymerase Chain Reaction statistics & numerical data, Sensitivity and Specificity, Serine Endopeptidases genetics, Sputum microbiology, Virulence, Allergens, Aspergillosis microbiology, Aspergillus fumigatus genetics, Genes, Fungal, Lung Diseases, Fungal microbiology
- Abstract
Asp fI(18 kDa) and alkaline protease (33 kDa) are the 2 major antigens which are derived from Aspergillus (A.) fumigatus and have been implicated as possible virulence factors in the pathogenesis of Aspergillus-induced diseases. We attempted to detect fragments of genes encoding both proteins from fungus balls obtained at surgery or autopsy by polymerase chain reaction (PCR) amplification and then used PCR to test clinical samples. Frozen-stored fungus ball samples from a patient with acute myeloid leukemia complicated by Aspergillus pneumonia and from a patient with pulmonary aspergilloma were studied. We successfully amplified a 315 bp PCR product, the target sequence for Asp f I, and a 747 bp PCR product as a target sequence for alkaline protease (ALP) in both cases. In addition, 13 clinical samples including sputum specimens from patients with pulmonary aspergillosis were also examined. PCR analysis for the Asp f I (ALP) gene in clinical samples showed positive results in 5/10 (6/10) patients with pulmonary aspergilloma and in 3/3 (1/ 3) patients with invasive pulmonary aspergillosis. Culture data on A. fumigatus revealed positive results in 3/9 patients with pulmonary aspergilloma and in 2/3 patients with invasive pulmonary aspergillosis. This method can be used to recognize the involvement of A. fumigatus in various clinical settings where conventional culture results are not readily available.
- Published
- 1997
- Full Text
- View/download PDF
45. Bloom's syndrome complicated by myelodysplastic syndrome and multiple neoplasia.
- Author
-
Iwahara Y, Ishii K, Watanabe S, Taguchi H, Hara H, and Miyoshi I
- Subjects
- Adult, Diabetes Complications, Humans, Male, Pedigree, Anemia, Refractory, with Excess of Blasts complications, Bloom Syndrome genetics, Neoplasms, Multiple Primary complications
- Abstract
The patient, a Japanese male born to a highly consanguineous family, was diagnosed as Bloom's syndrome at the age of 33 when he presented with diabetes mellitus and refractory anemia with excess blasts. Chromosome abnormalities of bone marrow cells included 5q-, -7/7q-, and unusual translocations. During the ensuing years, he developed squamous cell carcinoma of the external auditory meatus, adenocarcinoma of the colon, and squamous cell carcinoma of the tonsil. The patient died of pneumonia at the age of 38. Autopsy revealed intestinal polyposis and hemochromatosis secondary to massive blood transfusions.
- Published
- 1993
- Full Text
- View/download PDF
46. Neutralizing antibody to vesicular stomatitis virus (HTLV-I) pseudotype in infants born to seropositive mothers.
- Author
-
Iwahara Y, Sawada T, Taguchi H, Hoshino H, Umemoto M, Take H, Foung S, and Miyoshi I
- Subjects
- Adult, Female, HTLV-I Infections immunology, Humans, Infant, Newborn, Neutralization Tests, Serologic Tests, Viral Core Proteins immunology, Viral Structural Proteins immunology, Antibodies, Viral analysis, HTLV-I Antibodies analysis, Vesicular stomatitis Indiana virus immunology
- Abstract
Breast feeding is the major route of mother-to-child transmission of human T-cell leukemia virus type I (HTLV-I). Our experiments with rabbits have shown that passive immunization is capable of blocking cell-to-cell infection of HTLV-I by blood transfusion or breast feeding. In this study, sera were collected serially from 3 infants born to seropositive mothers and were tested for the presence of neutralizing antibody to vesicular stomatitis virus (HTLV-I) pseudotype as well as antibodies to viral structural proteins. There was a good correlation between neutralizing and viral antibody titers, both of which were detectable until 3-6 months after birth. Whether maternally transmitted neutralizing antibody is protective against perinatal infection of HTLV-I remains to be studied.
- Published
- 1993
- Full Text
- View/download PDF
47. Aberrant expression of the p53 tumor suppressor gene in adult T-cell leukemia and HTLV-I-infected cells.
- Author
-
Yamato K, Oka T, Hiroi M, Iwahara Y, Sugito S, Tsuchida N, and Miyoshi I
- Subjects
- Adult, Base Sequence, Cell Line, Gene Expression, Gene Products, tax analysis, Gene Products, tax genetics, Genes, pX, Humans, Immunoblotting, Molecular Sequence Data, Oligodeoxyribonucleotides, Polymerase Chain Reaction methods, Restriction Mapping, Tumor Cells, Cultured, Tumor Suppressor Protein p53 analysis, Tumor Suppressor Protein p53 genetics, Genes, p53, HTLV-I Infections genetics, Human T-lymphotropic virus 1 genetics, Leukemia-Lymphoma, Adult T-Cell genetics
- Abstract
By immunoprecipitation analysis, enhanced p53 expression was detected in 3 of 4 adult T-cell leukemia (ATL) cell lines, 1 of 3 HTLV-I-infected cell lines and 1 of 5 fresh ATL samples, compared with phytohemagglutinin-stimulated peripheral blood lymphocytes. Among these 5 high expressers, p53 missense mutations were indicated in 2 ATL cell lines and 1 fresh ATL sample by extensive p53 cDNA and genomic DNA polymerase chain reaction single-strand conformation polymorphism analysis. No mutation was found throughout the entire coding region of the remaining 2 high expressers (1 ATL and 1 HTLV-I-infected cell lines) and low expressers of p53 (2 HTLV-I-infected cell lines). Tax oncoprotein expression was found in these 2 high p53 expressers in which p53 mutation was not present, but not in low p53 expressers or cells carrying this mutation. The levels of p53 mRNA were similar among the samples regardless of p53 levels. Posttranscriptional mechanisms other than missense mutation would thus appear to increase p53 in the Tax-expressing cells but not in cells containing undetectable levels of Tax. No complex formation between p53 and Tax was observed.
- Published
- 1993
- Full Text
- View/download PDF
48. [Intensive induction chemotherapy of adult acute myelogenous leukemia by continuing daunorubicin, behenoyl-cytosine arabinoside, 6-mercaptopurine and prednisolone until marrow aplasia].
- Author
-
Taguchi H, Kubonishi I, Takehara N, Uemura Y, Iwahara Y, Eguchi T, Miyagi T, Sugito S, Muneishi H, and Tanaka Y
- Subjects
- Administration, Oral, Adolescent, Adult, Aged, Antineoplastic Combined Chemotherapy Protocols adverse effects, Cytarabine administration & dosage, Cytarabine adverse effects, Daunorubicin administration & dosage, Daunorubicin adverse effects, Drug Administration Schedule, Female, Humans, Infusions, Intravenous, Injections, Intravenous, Leukemia, Myeloid, Acute mortality, Male, Mercaptopurine administration & dosage, Mercaptopurine adverse effects, Middle Aged, Prednisolone administration & dosage, Prednisolone adverse effects, Remission Induction, Sepsis chemically induced, Survival Rate, Antineoplastic Combined Chemotherapy Protocols therapeutic use, Bone Marrow drug effects, Leukemia, Myeloid, Acute drug therapy
- Abstract
Intensive induction chemotherapy was applied to 25 patients with acute myelogenous leukemia by continuing drugs (daunorubicin, behenoyl-cytosine arabinoside, 6-mercaptopurine and prednisolone) until the achievement of severe bone marrow aplasia (leukemic cells less than 1,000/microliters). Complete remission (CR) was achieved in 18 (72%). Numbers of partial remission and an early death were 5 (20%) and 2 (8%), respectively. Although median nadirs of white blood cells (WBC) and platelet counts (Pl) (205/microliters and 8,200/microliters, respectively) were remarkably low, recovery of WBC (over 1,000/microliters) and Pl (over 50,000/microliters) were achieved in 23.8 and 24.5 days, after an initiation of the chemotherapy. Sepsis was a most frequently observed complication during induction stage and a duration of fever was 2-48 days (median 15). Median duration of CR was 22.9 months. Unexpectedly, 11 of 17 CR (except one with bone marrow transplanted) relapsed after 4.2-41.4 months (median; 9.4), but 6 (35.3%) still remain in first CR for 30.5-72.9 months (median; 51.4). A long-term survival might be obtained by intensifying induction chemotherapy in about one fourth of patients, but the intensification or application of non-cross resistant anti-leukemic agents in post-remission therapy may be required to avoid relapses even if induction is intensified.
- Published
- 1992
49. Excretion of HTLV-I in saliva.
- Author
-
Miyoshi I, Sawada T, Iwahara Y, Ishii K, Kubonishi I, and Taguchi H
- Subjects
- Adult, Aged, Carrier State, Female, Humans, Male, Middle Aged, HTLV-I Infections microbiology, Human T-lymphotropic virus 1 isolation & purification, Leukemia-Lymphoma, Adult T-Cell microbiology, Saliva microbiology
- Published
- 1992
50. Immunoglobulin prophylaxis against HTLV-I in a rabbit model.
- Author
-
Miyoshi I, Takehara N, Sawada T, Iwahara Y, Kataoka R, Yang D, and Hoshino H
- Subjects
- Animals, Deltaretrovirus Antibodies analysis, Disease Models, Animal, Gene Amplification, HTLV-I Infections genetics, HTLV-I Infections transmission, Humans, Rabbits, HTLV-I Infections prevention & control, Immunization, Passive
- Abstract
We have investigated the protective effect of human T-cell leukemia virus I (HTLV-I) immune globulin (HTLVIG) against HTLV-I in rabbits. HTLVIG containing 77 mg/ml of IgG was prepared from pooled plasma from seropositive healthy persons. In the first experiment, four groups (A, B, C, and D) of three rabbits were transfused with 5 ml blood from an HTLV-I-infected rabbit. Groups A, B, and C were infused 24 h later with 10, 5, and 2 ml HTLVIG, respectively, while group D was infused with 10 ml HTLVIG 48 h later. Seroconversion for HTLV-I occurred in none of group A, one of group B, and all of groups C and D after 2-5 weeks. In the second experiment, four litters (E, F, G, and H) born to another virus-infected rabbit and consisting of 7, 5, 7, and 7 newborns, respectively, were used. Litters E and H were allowed to grow normally as controls, while litters F and G were given intraperitoneal inoculation of 3 ml/kg of HTLVIG weekly four times until weaning. Although three of litters E and H each seroconverted after 5-8 weeks, none of litters F, and one of litter G became antibody-positive after 10 weeks. Presence or absence of HTLV-I infection in all these animals was confirmed by transfusion assay or gene amplification. These results indicate that passive immunization protects rabbits against blood- and milk-borne transmission of HTLV-I.
- Published
- 1992
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