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The aim of the present study was to investigate the use of homeopathic preparations to reduce the harmful effects caused by auxin herbicide residues on seed germination and early development of cucumber seedlings. The statistical design was completely randomized (DIC) in a 3x5+2 factorial scheme, with four replications. The treatments were the combination of three homeopathic preparations (Nux vomica, Carbo vegetabilis and Arsenicum album), in five centesimal dynamizations (6CH; 12CH; 18CH; 24CH and 30CH), plus two controls (T0= distilled water and TH = herbicide (Artys™) without homeopathic treatment). Each experimental unit consisted of transparent acrylic boxes (Gerbox™) with 25 cucumber seeds, distributed over two sheets of paper for germination and moistened with homeopathic treatments. The experimental units were kept in a BOD germination chamber (in a controlled environment at 25 ºC and 12 ho of light). On the tenth day after the installation of the experiment, the following variables were evaluated: percentage of germination, shoot length, root length, phytointoxication, percentage of dead seedlings and total dry mass. The homeopathic treatment of cucumber seeds contaminated with picloram + 2,4-D herbicide (Artys™) causes positive changes, causing interesting effects on seed germination, but it is not efficient for the total neutralization of the effect caused by the herbicide 2 picloram + 2,4-D and development of early cucumber seedlings. However, for some variables, the results are inconclusive with the preparations (Nux vomica, Carbo vegetabilis and Arsenicum album), and their ultradilutions (6CH, 12CH, 18CH, 24CH and 30CH), requiring more research to evaluate and describe the complexity of properties inherent to homeopathic treatment.

Amazonian savannas are isolated patches of open habitats found within the extensive matrix of Amazonian tropical forests. There remains limited evidence on how Amazonian plants from savannas differ in the traits related to drought resistance and water loss control. Previous studies have reported several xeromorphic characteristics of Amazonian savanna plants at the leaf and branch levels that are linked to soil, solar radiation, rainfall and seasonality. How anatomical features relate to plant hydraulic functioning in this ecosystem is less known and instrumental if we want to accurately model transitions in trait states between alternative vegetation in Amazonia. In this context, we combined studies of anatomical and hydraulic traits to understand the structure–function relationships of leaf and wood xylem in plants of Amazonian savannas. We measured 22 leaf, wood and hydraulic traits, including embolism resistance (as P50), Hydraulic Safety Margin (HSM) and isotope-based water use efficiency (WUE), for the seven woody species that account for 75% of the biomass of a typical Amazonian savanna on rocky outcrops in the state of Mato Grosso, Brazil. Few anatomical traits are related to hydraulic traits. Our findings showed wide variation exists among the seven species studied here in resistance to embolism, water use efficiency and structural anatomy, suggesting no unique dominant functional plant strategy to occupy an Amazonian savanna. We found wide variation in resistance to embolism (−1.6 ± 0.1 MPa and −5.0 ± 0.5 MPa) with species that are less efficient in water use (e.g. Kielmeyera rubriflora, Macairea radula, Simarouba versicolor, Parkia cachimboensis and Maprounea guianensis) showing higher stomatal conductance potential, supporting xylem functioning with leaf succulence and/or safer wood anatomical structures and that species that are more efficient in water use (e.g. Norantea guianensis and Alchornea discolor) can exhibit riskier hydraulic strategies. Our results provide a deeper understanding of how branch and leaf structural traits combine to allow for different hydraulic strategies among coexisting plants. In Amazonian savannas, this may mean investing in buffering water loss (e.g. succulence) at leaf level or safer structures (e.g. thicker pit membranes) and architectures (e.g. vessel grouping) in their branch xylem.

Tropical monodominant forests are rare communities with low tree species diversity. Species monodominance is not the product of a single mechanism, but the result of a set of not yet fully understood integrated ecological factors acting together. We compared populations of Brosimum rubescens in monodominant and mixed forests in Southern Amazonia to test whether leaf functional traits are ecological factors related to monodominance. Individuals of B. rubescens in the mixed forest invest in conservative strategies, while those in the monodominant forest invest in acquisitive strategies. Leaf functional traits, such as petiole length and adaxial cuticle thickness, could be associated with the monodominance of B. rubescens. Our study highlights for the first time the power of integrating leaf functional traits as a component of the set of ecological conditions to explain species monodominance. B. rubescens showed different functional strategies to establish and maintain its population in different forests, which makes it a strong competitor for resources, such as water and light, through variation in its leaf functional traits. We also suggest that such high plasticity can be an important condition for the persistence of the species over time.

Despite the uniqueness and reach of the flora from natural savannas in the Brazilian Amazon, and the existence of studies on its origin and diversity, there are no local studies associating floristic patterns with soil properties in savanna enclaves in the Amazon region of the state of Mato Grosso. Floristic composition and diversity were compared between a woody community from a rocky savanna inselberg in a transition region (RTS) between the two largest South American biomes (Cerrado-Amazon), and an enclave of rocky savanna in the Amazon (RAS), and the effects of soil properties were investigated. Floristic comparisons were also made between the two studied communities and two other rocky savanna communities near the Cerrado-Amazon transition. The flora and physical and chemical soil properties in twenty-five 20 × 20 m subplots (1 ha) in each community were sampled and georeferenced. An evident floristic distinction was found between the two studied communities, with low similarity values and a high number of indicator species. The observed and estimated richness and Rényi diversity profiles indicated lower species diversity in RAS than in RTS. Soils were found to be litholic, poorly drained, dystrophic, alic, extremely acidic, sandy and nutrient poor. Species composition and abundance was associated with soil properties in both communities. The clear difference in species composition and diversity between RTS and RAS seem to be shaped by soil properties, geographic isolation and floristic influence from the Cerrado and the Amazon. These results broaden the knowledge regarding the composition and diversity of woody plants of savannas in Amazonian enclaves and Cerrado inselbergs, and provide an important set of floristic and edaphic descriptors for the phytogeography of rocky savannas.

In Ant-gardens, ants build nests with organic materials and take specific seeds of some species of epiphytes which germinate. In this mutualistic interaction, a variety of other epiphytes could colonize the nests and develop, but it doesn't happen. We assume that there is chemical allelopathy with epiphytes inhibiting the germination and growth of other species of epiphytes, and with ants cutting off non-mutualistic epiphyte species. To investigate this, we performed chemical and prune tests which determine the composition of the epiphyte species of Ant-Gardens of parabiotic ants, Camponotus femoratus (Fabricius, 1804) and Crematogaster levior Longino, 2003. For the chemical allelopathic test, we administered the extract of the stem and leaves of Peperomia macrostachya (Vahl) A. Dietr. and Codonanthe calcarata (Miq.) Hanst. (the most common species in parabiotic AGs) in different concentrations. For the prune test, we used seedlings of the mutualistic plant Peperomia macrostachya and non-mutualistic plant Cucumis sativus as a control, then we inspected the nests to evaluate the ants pruning the seedlings. In the chemical allelopathy tests, the species Codonanthe calcarata decreased the germination speed indexes in relation to the control (distilled water). On the contrary, the length and weight of the seedlings were positively influenced by epiphyte extract. In the prune test, most of the plants pruned were non-mutualistic. The results of the chemical allelopathy and prune experiments showed that both mutualistic epiphytes and ants play a decisive role in the composition of epiphytes in Ant-Gardens.

Produzir tomates na Amazônia é um desafio, devido às altas temperaturas e precipitação. O objetivo deste estudo foi identificar o híbrido de tomateiro com maior desempenho produtivo e qualidade de fruto sob cultivo protegido em condições de altas temperaturas. O cultivo foi realizado em sistema tutorado em meia estaca, sem podas, sob cultivo protegido do tipo arco, em Nova Mutum – MT. O delineamento experimental adotado foi de blocos ao acaso, com cinco repetições. Foram aferidos parâmetros produtivos, físico-químicos, bioquímicos de frutos e a anatomia foliar. O híbrido ‘Lampião’ ‘Fascínio’, ‘Vedette’ e ‘Shanty’ se destacaram em produção e qualidade nutricional, comparado aos demais. Os parâmetros da anatomia foliar analisados demonstraram alterações adaptativas à luminosidade difusa, umidade e temperatura, não demonstrando correlação com a produção dos híbridos avaliados. Quanto a coloração do fruto, o híbrido ‘Candieiro’ apresentando coloração mais vermelha. O híbrido ‘Mariana’ mostrou menor desempenho em produtividade, altura da planta e concentração de licopeno, demonstrando menor adaptação ao ambiente protegido sob altas temperaturas.

The presence of weeds amid pastures has caused significant damage to cattle farmers due to the difficulty in controlling these species. Paspalum virgatum stands out as an aggressive weed in Amazonian agroecosystems. Proper management of weed species is threatened by their aggressiveness, adaptation to low fertility soils and morphological, physiological and biochemical similarities with the grasses used as fodder. It is also possible that part of these characteristics of aggressiveness occur due to the ability of weeds to produce allelochemicals, resulting in damage to surrounding plants. The objective of this study was to qualitatively verify the presence of secondary metabolites in P. virgatum species under different water availability. The analyzed species were initially grown separately under three conditions of water availability: water deficit, field capacity and flooded environment. The water deficit treatment was conducted to verify weed ability to develop under conditions of extreme water shortage. This is a characteristic related to its rusticity, as found in grasses in the Amazon region. Excess water treatments were also conducted, since this is a common condition found in areas with partial flooding of a few months during the rainy season in the Amazon. After 120 days, weeds were collected and separated into roots, stems and leaves. The material was subsequently dried, ground and subjected to procedures to determine secondary metabolites. Phenolic compounds, flavonoids and alkaloids were identified in all conditions of plant development. The flavonoid detection test presented an intense yellow color. This is a strong indication of the presence of this class in stems and leaves of plants under flood and in leaves of plants under field capacity soil conditions. An intense presence of phenolic compounds was verified in stems and leaves, regardless of treatment. General alkaloids were found in great intensity in all samples. Saponins were found in leaves of P. virgatum under water stress (flooding and water deficit). All the metabolites were found in the plants, diverging between treatments and parts of the plant. It was concluded that the species presented higher steroid content in the roots when growing in places with excess of water. In conditions of full water supply, the presence of steroid was less intense in the roots, leaves and stems, result also found in roots and leaves of plants under water deficit. Alkaloids were less intensely present in the roots, regardless of the water treatment in which the plant was submitted. Results demonstrated that there are secondary metabolites responsible for some physiological ability of the weed to withstand excess or water deficit, indicating the need for further studies

This study aimed to understand the functionality and structural adjustments of wood in a rocky-outcrop plant community in an Amazonian savannah by correlating anatomical traits of wood associated w...

Entre as interações mutualísticas envolvendo formigas e plantas, o exemplo mais complexo conhecido chama-se jardins de formigas. Nesta interação, as formigas constroem o ninho na vegetação, plantam sementes de epífitas e as protegem, quando crescem. Em troca, as epífitas controlam a umidade do ninho, oferecem estrutura para o seu crescimento e alimento para as formigas. Neste artigo, contextualizamos e situamos o leitor quanto à interação mutulística denominada de jardins de formigas, compilamos informações disponíveis na literatura e trazemos dados de pesquisas ainda não publicadas, mas em andamento, sobre este assunto.

Alpinia spp. play an important ecological role as a source of nectar in the understory of tropical and subtropical forests and many are common. The perennial species Alpinia purpurata (Vieill.) K. Schum. is native to the tropical rainforests of Asia and has been used as an ornamental cut plant because of its lush inflorescences and post-harvest durability. The internal and external morphology, floral biology, and reproductive system of four A. purpurata cultivars were investigated to contribute to studies on the genetic improvement of this species. The inflorescence of A. purpurata is terminal and globose, and has flowers protected by colorful and showy bracts. The white flowers are tubular and bisexual. The floral anthesis of these four cultivars lasted 9 to 12 h, and the pollen viability was above 80% throughout the pre-flowering period in all evaluated cultivars. During anthesis, the stigma and anther crest were receptive because of the presence of stigmatic fluid. Fruiting was observed after hand cross-pollination, whereas no fruiting was recorded in the treatments with spontaneous self-pollination, hand self-pollination or geitonogamy. Natural pollination induced low fruiting, suggesting low pollinator availability in the studied area. The obligatory xenogamy of A. purpurata facilitates the development of new hybrids for the ornamental plant market. RESUMEN Alpinia spp. desempeñan un importante papel ecológico, como fuente de néctar, en el sotobosque de los bosques tropicales y subtropicales, donde muchas de estas especies son comunes. La especie perenne Alpinia purpurata (Vieill.) K. Schum. es nativa de las selvas tropicales de Asia y se ha utilizado como planta de corte ornamental debido a las exuberantes inflorescencias y la durabilidad posterior a la cosecha. Se investigó la morfología interna y externa, la biología floral y el sistema reproductivo de cuatro cultivares de A. purpurata, para apoyar los estudios de mejora genética de la especie. La inflorescencia de A. purpurata es terminal, globosa y tiene flores protegidas por brácteas coloridas y vistosas. Las flores blancas son tubulares bisexuales. La antesis floral de los cuatro cultivares duró entre 9 y 12 h. La viabilidad del polen fue superior al 80% durante todo el período de pre-floración en todos los cultivares evaluados. Durante la antesis, el estigma y la cresta de la antera fueron receptivos. La fructificación de los cultivares se observó después de la polinización cruzada manual. No se registró fructificación en los tratamientos de autopolinización espontánea, autopolinización manual y geitonogamia. La polinización natural indujo baja fructificación, lo que sugiere una baja disponibilidad de polinizadores en el área estudiada. La xenogamia obligatoria de A. purpurata es un facilitador para el desarrollo de nuevos híbridos para el mercado de plantas ornamentales.

O objetivo deste trabalho foi determinar a influência da procedência, tipo de estaca e da concentração de ácido indol-3-butírico (AIB) no enraizamento de estacas de mangabeira. As procedências testadas foram: Cáceres e Chapada dos Guimarães, Mato Grosso, das quais foram obtidas estacas herbáceas e lenhosas de árvores adultas. As estacas foram imersas em soluções de AIB nas concentrações de 0, 2.000, 4.000, 6.000, 8.000 e 10.000 mg.L-1 por 10 segundos e plantadas em bandejas de poliestireno expandido contendo areia lavada como substrato. O cultivo ocorreu em casa de vegetação por 60 dias. Análises anatômicas foram realizadas para investigação de possíveis impedimentos à iniciação radicial. O delineamento utilizado foi o inteiramente casualizado em arranjo fatorial 6 x 2 (seis concentrações de AIB e duas procedências), com 4 repetições de 10 estacas por unidade experimental. Não houve enraizamento das estacas de mangabeira, sendo constatada mortalidade total das mesmas. A dificuldade enraizamento da espécie não pode ser justificada pela estrutura anatômica do caule, o qual não possui qualquer impedimento à emissão de raízes.

Weeds are species evolutionarily adapted to development in areas of disorders caused by man or not. Adoption of practices to control and integrated weed management, it is essential the knowledge related to germination and environmental factors that interfere with seed germination processes. In this context, the present work aimed to evaluate the behavior of Macroptilium lathyroides seeds in response to different levels of osmotic potential induced NaCl and CaCl2 solutions. The experiment has been organized in a completely randomized design in a factorial 2 x 6, consisting of two salts in concentrations 6 with 4 repetitions. The seeds were kept in on moistened gerboxes germibox roles with NaCl and CaCl2 solutions at the potentials of 0.0; - 0.2; - 0.4; -0.6; -0.8 and -1.0 MPa. Daily reviews by germination, IVG and end ten days were determined shoot length, root length and plantlet dry weight were performed. The results demonstrated that salt stress induced by NaCl and CaCl2 provokes a gradual reduction in germination of M. lathyroides there is no tolerance limit. The NaCl The salt was more critical root development and of the seedlings dry weight.

A descoberta das propriedades terapêuticas das plantas é algo antigo, mas para sua utilização, precisa-se identificar as espécies corretamente, e muitas vezes, caracteres morfológicos não são o suficiente. Portanto, o objetivo deste trabalho foi diferenciar através da anatomia, histoquímica e fitoquímica três espécies do gênero Ocimum, amplamente utilizadas para produção de remédios naturais. Para tanto, foram coletadas Ocimum basilicum, O. gratissimum e Ocimum campechianum em quintais urbanos no município de Alta Floresta, estado de Mato Grosso, Brasil. Anatomicamente as espécies Ocimum basilicum e O. gratissimum são similares, enquanto a diferença de O. campechianum se dá pelo número de feixes vasculares e estrutura de raiz. Os testes histoquímicos mostraram a semelhança nos compostos produzidos de potencial medicinal, assim como a fitoquímica revelou a presença de taninos, flavonoides e alcaloides, compostos antioxidantes, expectorantes e anestésicos. A partir dos resultados obtidos, podemos concluir que as espécies possuem anatomia similar assim como a presença de compostos secundários, os quais são tradicionalmente indicados para uso medicinal.

A alelopatia é uma estratégia da planta para sua defesa e delimitação territorial, muito comum em plantas daninhas. O gervão (Stachytarpheta cayennensis) é uma espécie de planta daninha frequente em áreas de cultivo e urbanas na região amazônica do Brasil. Poucos trabalhos sobre alelopatia têm destacado as alterações anatômicas que os metabólitos secundários causam nas plantas afetadas. O objetivo desse trabalho foi analisar a ação alelopática de cinco concentrações de extrato alcoólico (0; 0,5; 1; 2; e 4%) de S. cayennensis a nível de alterações anatômicas em plântulas de Cucumis sativus. Os resultados evidenciaram aumento de espessura nas estruturas anatômicas dos órgãos analisados de pepino em extratos aquosos e alcoólicos, demonstrando acentuado espessamento conforme o aumento das concentrações dos extratos da espécie em estudo. A confirmação de que a liberação dessas substâncias intervêm no desenvolvimento de C. sativus serve como importantes informações a serem utilizadas no desenvolvimento de estratégias alternativas ao uso de herbicidas em outras culturas, visando o manejo ecológico de plantas daninhas.

Qualea genus is commonly found in different phytophysiognomies of the Brazilian Cerrado, growing in open ecosystems to closed forest areas, under high irradiances, fires, low availability of nutrients and seasonal water deficit. We compared the leaf structural traits of Qualea grandiflora Mart., Qualea multiflora Mart., and Qualea parviflora Mart., in a cerradão area in the Cerrado-Amazonian forest transition. Leaf samples were collected at Mário Viana Municipal Biological Reserve, in Nova Xavantina, Mato Grosso state, Brasil, and processed according to the usual techniques for optical microscopy. Thick cuticle, stomatal ridges, stomata distributed between the stomatal crypts, density and distribution of tector trichomes, hypodermis, isobilateral mesophyll with few intercellular spaces, abundance of sclerenchyma associated to vascular bundles and the presence of vascular bundle sheath extensions are typical anatomic traits of xerophytic environments. We found qualitative structural differences among the species, with Qualea grandiflora having stomata with ridges distributed among stomatal crypts, and the presence of a hypodermis in Qualea multiflora, and Qualea parviflora. The results suggest that the species are xeromorphic in the region of Cerrado-Amazonian transition having leaf adaptive traits to deal with the environmental variations of their habitat.

Eumachia DC. é um gênero de Rubiaceae que recentemente teve sua circunscrição ampliada devido a estudos baseados em dados moleculares e distribuição geográfica. Rubiaceae, representanda por 13.235 espécies distribuídas em 614 gêneros é uma das maiores famílias de Angiospermas. Diante dessa grande diversidade e presença em diferentes estratos vegetacionais, espécies da família Rubiaceae possuem grande importância para estudos relacionados a ecologia, conservação e preservação em regiões tropicais. A anatomia ecológica pode ser uma abordagem útil para compreender as mudanças estruturais e entender os processos de colonização e sobrevivência de espécies. O objetivo deste estudo foi comparar a anatomia foliar de Eumachia kappleri (Miq.) Delprete & J.H. Kirkbr., ocorrente em três diferentes áreas, buscando identificar caracteres-chave que mostrem estratégias adaptativas em seus ambientes naturais. Os indivíduos foram coletados em três áreas inseridas em uma região onde ocorrem duas estações climáticas definidas. Para o estudo dos caracteres anatômicos, em cada área foram identificados três indivíduos e, destes, coletadas cinco folhas adultas, antes totalmente expostas ao sol, que foram fixadas em FAA50 por 48 horas e depois conservadas em etanol 70%. Posteriormente, com técnicas usuais em anatomia vegetal e com uso de lâminas de aço, foram obtidas secções transversais e procedeu-se a dissociação de epidermes. Lâminas histológicas semi-permanentes foram montadas, para visualização e registro fotográfico em fotomicroscópio fotônico trinocular acoplado a um computador. Para o estudo quantitativo serviram onze atributos anatômicos, analisados com o auxílio do software de análise de imagem. Após normalizar os dados necessários, uma matriz de correlação foi construída e posteriormente uma MANOVA foi realizada. Após identificar quais variáveis colaboraram para a significância da MANOVA, foi aplicado o teste de Tukey. Através da MANOVA foi possível identificar diferença significativa entre as áreas. Na maioria das variáveis morfoanatômicas os indivíduos da área Reserva FA (Reserva do Floresta Amazônica Hotel Resort) apresentaram medidas significativamente maiores que as outras duas áreas. A análise da anatomia foliar mostrou que E. kappleri apresenta características anatômicas que são consideradas comuns à família, porém quando comparadas em ambientes distintos, diferem em alguns aspectos. Conclui-se que algumas das variações anatômicas entre as amostras analisadas como espessura de epiderme e mesofilo favorecem a aclimatação da espécie às clareiras florestais, tornando-a bem ajustada em ambientes abertos.

Indroduction Rupture of and abdominal aortic aneurysm (AAA) in a kidney transplant patient is a rare and rarely reported event. Emergent treatment can be challenging and should achieve effective aortic repair while minimizing ischemic damage to the renal graft during aortic cross-clamping. Several renal protective measures have been proposed such as permanent or temporary shunts, renal cold perfusion and general hypothermia. Case Report We report the effective treatment of a para-renal AAA in a patient with a functional renal allograft. A temporary extra-corporeal axillofemoral shunt was constructed to maintain graft's perfusion during open surgical repair. EVAR was not an option due to a short aortic neck. The postoperative period was complicated by colon ischemia and aortic graft infection. At 3 years follow-up the patient was well and graft's function was unchanged. Conclusion This case is a reminder that renal graft protection must be accounted for when AAA rupture occurs in kidney transplant patients. We reviewed the literature to find previously reported cases and how they were managed.

Resumo A plasticidade fenotípica geralmente observada em caracteres morfológicos e anatômicos foliares pode ser influenciada por diversos fatores, como luminosidade, disponibilidade de nutrientes no solo, umidade e herbivoria, entre outros. Testamos essa plasticidade em Miconia nervosa (Melastomataceae) em um fragmento florestal no sul da Amazônia, Mato Grosso - Brasil, buscando verificar possíveis estratégias de aclimatação morfoanatômica à luminosidade. Coletamos folhas totalmente expandidas de 15 indivíduos adultos expostos diretamente à luz solar, nas margens de um lago, e 15 sob o sombreamento do dossel. Avaliamos quantitativamente oito variáveis morfológicas e seis anatômicas, das quais seis e quatro, respectivamente, diferiram significativamente entre folhas de sol e sombra. Morfologicamente, as folhas de sol apresentaram maior tamanho da lâmina foliar e do pecíolo e, anatomicamente, células epidérmicas com paredes levemente mais espessadas, maiores densidades e índice estomático. Folhas de sombra apresentaram epiderme significativamente mais espessa em ambas as faces. As folhas de M. nervosa apresentaram grau mediano a alto de plasticidade nas variáveis morfoanatômicas conforme índice de plasticidade fenotípica, com caracteres mistos de ambientes de sol e sombra. Sugerimos que a distribuição dos indivíduos de M. nervosa na floresta está relacionada à disponibilidade de luz e umidade do solo. Abstract The phenotype plasticity usually observed in morphological and anatomical leaf traits may be influenced by several factors, such as luminosity, soil nutrient availability, humidity and herbivory, among others. We tested leaf plasticity in Miconia nervosa (Melastomataceae) in a forest fragment in Southern Amazonia, Mato Grosso State - Brazil, seeking to verify morpho-anatomical strategies of light acclimation. We collected fully expanded leaves of 15 individuals exposed directly to sunlight, on a lake edge, and 15 under canopy shading. We evaluated quantitatively eight morphological variables and six anatomical variables, of which six and four, respectively, differed significantly between sun and shade leaves. Morphologically, sun leaves showed larger leaf and petiole sizes and anatomically, epidermal cells with slightly thickened walls, higher stomatal index and density. Shade leaves showed significantly thicker epidermis on both leaf faces. Leaves of M. nervosa exhibited medium to high degree of plasticity in morpho-anatomical traits, according to the plasticity index, with mixed characteristics of sun and shade environments. We suggest that the distribution of M. nervosa individuals in the forest is related to the availability of light and soil moisture.

Made available in DSpace on 2022-04-28T19:29:52Z (GMT). No. of bitstreams: 0 Previous issue date: 2020-09-01 Green manure stands out in the organic vegetable production as complementary fertilizer alternative to organic compound incorporation, contributing to reduce production costs and improve soil physical, chemical and biological features. The aim of the current study is to assess green manure and spontaneous vegetation species in cropping systems focused on the organic cabbage production, as well as their influence on soil microbial community. The treatments consisted of green manures such as black velvet beans (Stizolobium aterrimum), Crotalaria spectabilis, jack beans (Canavalia ensiformis), pigeon peas (Cajanus cajan cv. Iapar 43) and spontaneous vegetation (fallow) in two cropping systems (with and without incorporation of green manure plants). Evaluations of green manure, cabbage crop and soil microbial activity were carried out. Green manure: fresh and dry mass, nitrogen and accumulated nitrogen content; Cabbage culture: horizontal and vertical diameter of the head, commercial and total mass; Microbial soil activity: soil microbial biomass carbon, soil basal respiration and metabolic quotient. For fresh and dry mass, jack beans and pigeons pea presented higher values, indicating good performance of these materials. For both, nitrogen content and accumulated nitrogen, the jack beans were stood. Regarding cabbage culture, the spontaneous vegetation decreased the horizontal diameter of the head when the incorporation did not occur. As for the commercial mass, for jack beans the cultivation with incorporation was superior. When the incorporation of the covering plants occurred, the crotalaria spectabilis produced less commercial mass and total head mass. Still in relation to total head mass, jack beans and spontaneous vegetation had lower mass when no incorporation occurred. As to soil microbial biomass carbon the spontaneous vegetation presented higher value, differing only from black velvet beans. The use of jack beans as a cover crop associated with the incorporation system was shown to be promising for the production of cabbage. Postgraduate Program in Environments and Production Systems Mato Grosso State University, Tangará da Serra Postgraduate Program in Amazonian Biodiversity and Agroecosystems Mato Grosso State University, Alta Floresta Postgraduate Program in Agriculture Paulista State University Postgraduate Program in Agriculture Paulista State University

No presente trabalho, foi avaliado o efeito da salinidade sobre a germinação de sementes e desenvolvimento pós-seminal de Ochroma pyramidale através de três agentes osmóticos (NaCl, CaCl2 e KCl), em dois experimentos. No primeiro, foi avaliado o estresse salino na germinação em esquema fatorial de 3 x 6, utilizando NaCl, CaCl2 e KCl, e seis potenciais osmóticos (0,0; -0,1; -0,2; -0,4; -0,6 e -0,8 MPa). No segundo experimento, estudou-se o crescimento inicial de plântulas, utilizando os mesmos agentes osmóticos, em quatro potenciais osmóticos (0,0; -0,1; -0,2 e -0,3 MPa), no delineamento inteiramente ao acaso esquema fatorial 3 x 4. O efeito da salinidade interferiu na germinação de sementes O. pyramidale, havendo redução à medida que os potenciais se tornaram mais negativos à produção de massa seca de plântula, percentual de germinação e velocidade do processo germinativo. No potencial osmótico de -0,8 MPa, ocorreu inibição total, evidenciando que o CaCl2 apresentou um efeito inibitório mais acentuado que o NaCl, seguido pelo KCl. No desenvolvimento de plantas, o NaCl provocou maior sensibilidade, comparando-se ao CaCl2 nos maiores potenciais osmóticos.Palavras-chave: Pau-de-balsa; germinabilidade; potencial osmótico; estresse salino. EFFECT OF SALINITY IN THE GERMINATION AND DEVELOPMENT OF Ochroma pyramidale SEEDLINGS ABSTRACT: In the present study, the effect of salinity on seed germination and post-seminal development of Ochroma pyramidale was evaluated through three osmotic agents (NaCl, CaCl2 and KCl) in two experiments. In the first experiment, salt stress in germination was evaluated in a factorial scheme 3 x 6, using NaCl, CaCl2 and KCl; and six osmotic potentials (0.0, -0.1, -0.2, -0.4, -0.6 and -0.8 MPa). In the second experiment, the initial development of plants, using the same osmotic agents, was studied in four osmotic potentials (0.0; -0.1; -0.2 and -0.3 MPa), in a completely randomized design 3 x 4 factorial scheme. The effect of salinity interfered on the germination of O. pyramidale seeds, with reduction as the potentials became more negative for the production of dry seedling mass, percentage of germination and germination speed. In the osmotic potential of -0.8 MPa, total inhibition occurred, evidencing that CaCl2 had a stronger inhibitory effect than NaCl, followed by KCl. In the development of plants, NaCl caused greater sensitivity when compared to CaCl2 in the highest osmotic potentials.Keywords: Pau-de-Balsa wood; germinability; osmotic potential; saline stress.

Resumo Este estudo objetivou caracterizar a morfoanatomia, histoquímica e fitoquímica dos órgãos vegetativos de Hymenea courbaril, visando detectar estruturas secretoras ou células secretoras que armazenam metabólitos secundários, cujas atribuições conferem potencial medicinal à espécie. A coleta do material vegetal foi realizada em indivíduos nativos de H. courbaril no município de Alta Floresta, MT. O material botânico (folíolos e caule) foi fixado, foram realizadas secções transversais e paradérmicas nos órgãos, montadas em lâminas histológicas e o material fresco foi submetido a testes histoquímicos e fitoquímicos. Foram detectados canais de secreção no folíolo e caule de H. courbaril, a caracterização fito-histoquímica deu positivo para metabólitos secundários como taninos, esteroides, flavonoides, compostos fenólicos, lipídios, saponinas e glicosídeos, a presença destes compostos pode conferir à espécie atividades biológicas importantes no tratamento de diversos tipos de doenças. Além disso, tais substâncias desempenham um importante papel ecológico de manutenção e continuidade da espécie na natureza, como mecanismos contra a herbivoria e a desidratação. Abstract This study aimed to characterize the morphology, histochemistry and phytochemistry of the vegetative organs of Hymenaea courbaril, aiming to detect secretory structures and secretory cells that store secondary metabolites, whose attributions confer medicinal potential to the species. The collection of plant material was carried out in native individuals of H. courbaril in the municipality of Alta Floresta, MT. The plant material (leaflets and stem) was fixed, transverse and paradermic sections were performed on the organs, mounted on histological slides, and the fresh material was submitted to histochemical and phytochemical tests. Secretion channels were detected in the leaflet and stem of H. courbaril, the phyto-histochemical characterization proved positive for secondary metabolites such as tannins, steroids, flavonoids, phenolic compounds, lipids, saponins and glycosides, the presence of these compounds may confer important biological activities in the treatment of various types of diseases. In addition, such substances present an important ecological role in the maintenance and continuity of the species in nature as mechanisms against herbivory and dehydration.

The diversity of environments in the Neotropical region has different environmental filters on vegetation. The southern face of the Amazon is exposed to high rainfall seasonality and poor soils, which imposes a severe environmental filtering. Plants with structural and functional adaptations can occur in specific locations due to microenvironmental characteristics. We investigated how anatomical-leaf attributes of seven co-occurring species of savanna and forest formations respond to the environmental conditions of their phytophysiognomies using usual optical microscopy techniques and statistical models. The species have different strategies to support environmental conditions. Even under a more limiting rainfall regime, plants living in seasonal forests closely related with savannic areas (Cerradao area - CDA) are less endowed with xeromorphic characteristics, which we can attribute to the conditions of continuous canopy and soil, comparatively more clayey than savanna soils. The species associated with rocky outcrops more closely related to savannic habitats (Rupestrian Cerrado of Transition area - RCT) showed greater investment in thickness of epidermal cell walls and the total thickness of the central vein. These species, together with the species associated with rocky outcrops more closely related to forest habitats (Amazon Savanna on Rocky Outcrops area - ASR), demonstrated greater investment in cuticle, straight anticline walls, straight periclinal walls or straight and convex periclinal walls, epidermis thickness, parenchyma, secretory ducts, collenchyma and sclerenchymatous fibers. Our findings reveal species’ different responses to environmental conditions: ASR and RCT species present a likely longer (temporal) selective process with a high investment in hydraulic safety, but RCT species showed greater xeromorphism; CDA forms a less xeromorphic microenvironment. The leaf xeromorphism is not only determined by the rainfall levels or soil fertility characteristics, but also by the degree of exposure of these plants to solar radiation and local seasonality.

O conhecimento anatômico de espécies do gênero Theobroma é importante para o estabelecimento e manutenção de coleções de germoplasma, tendo em vista estudos agronômicos e fitopatológicos e programas de melhoramento genético. Este estudo objetivou caracterizar a anatomia das folhas de Theobroma speciosum, registrando as diferenças estruturais observadas entre folhas de sol e folhas de sombra. Folhas de indivíduos de Theobroma speciosum foram coletadas no Parque Nacional do Juruena e em pastagens na zona rural do município de Alta Floresta – MT. Foram analisados a espessura do mesofilo foliar, dos parênquimas paliçádico e lacunoso, da nervura mediana e do feixe vascular. A análise de agrupamento foi realizada utilizando o método Hierárquico UPGMA, com base na distância euclidiana média padronizada. Foram observados tricomas glandulares do tipo claviforme e estrelados sésseis. A epiderme é unisseriada, o mesofilo é dorsiventral, entretanto os parênquimas são pouco distintos. Feixes vasculares colaterais estão dispersos no mesofilo, formando bainhas que se estendem até as epidermes. A nervura central, em secção transversal, possui formato biconvexo, com sistema vascular fechado envolto por bainha esclerenquimática. A constituição do mesofilo das folhas variou com o ambiente. Plantas expostas a pleno sol apresentam células mais alongadas no parênquima paliçádico, parênquima lacunoso com mais camadas e maior quantidade de tricomas, enquanto que as folhas de sombra apresentaram células com muitos espaços intercelulares no mesofilo. O dendrograma mostrou a formação de dois grupos distintos: o grupo I composto pelas plantas do Parque e o grupo II pelas plantas das pastagens. Tal agrupamento demonstrou alterações anatômicas significativas ocasionadas pelas variações da intensidade luminosa sobre as folhas, o que evidenciou grande capacidade adaptativa de Theobroma speciosum.

Roots and leaves of Panicum maximum Tanzânia, Mombaça and Massai; Urochloa brizantha Piatã, Marandu and Xaraés; Urochloa humidicola Llanero; Urochloa ruziziensis Ruzizienses; Urochloa hybrida Mulato II and Cynodon nlemfuensis Estrela-roxa were analyzed, seeking to identify characters for better adaptation to the environment that may interfere with digestibility of tissue from the point of view of the rumen in cattle. Were planted ten cultivars in a completely randomized blocks with three repetitions. Was collected vegetative material, which histological slides were prepared from middle third of the sections of roots and leaves. Were observed differences (p>0.05) in the roots: higher volume of epidermal cells (28.62 µm) and overall diameter (1926.41 µm) of Llanero; thicker vascular cylinder (975.09 µm) and more protoxylem (42.25) in Estrela-roxa and occurrence of aerenchyma in cultivars Piatã, Mulato II, Xaraés, Massai, Llanero and Estrela-roxa; Were found higher proportions of bulliform cells in the leaves (121.07 µm) and thicker leaf mesophyll in U. humidicola Llanero (263.63 µm); higher proportion of sclerenchyma fibers in Xaraés and Marandu; lower results for amount of fibers in P. maximum Massai. We conclude that the cultivars Estrela-roxa, Llanero and Massai have greater adaptability to the environment and better nutritional quality. Resumo Foram analisadas raízes e folhas de Panicum maximum Tanzânia, Mombaça e Massai; Urochloa brizantha Piatã, Marandu e Xaraés; Urochloa humidicola Llanero; Urochloa ruziziensis Ruzizienses; Urochloa hybrida Mulato II e Cynodon nlemfuensis Estrela-roxa, procurando identificar caracteres relacionados à melhor adaptação ao ambiente e à qualidade nutritiva das forrageiras. As dez cultivares foram semeadas em blocos inteiramente casualizados com três repetições. Após estabelecidas foi coletado material vegetativo, do qual lâminas histológicas foram confeccionadas a partir de secções do terço médio de raízes e folhas. Foram observadas diferenças significativas (p>0,05) nas raízes: maior volume de células epidérmicas (28,62 µm) e diâmetro total (1926,41 µm) de Llanero; em Estrela-roxa maior espessura do cilindro vascular (975,09 µm) e número maior de protoxilemas (42,25) e formação de aerênquimas nas cultivares Piatã, Mulato II, Xaraés, Massai, Llanero, e Estrela-roxa; Nas folhas, foram constatadas maiores proporções de células buliformes (121,07 µm) e mesofilo foliar mais espesso (263,63 µm) em Llanero; em Xaraés e Marandu maiores proporções de fibras esclerenquimáticas; em Massai menores resultados para quantidade de fibras. Conclui-se que as cultivares Estrela-roxa, Llanero e Massai apresentam maior adaptabilidade ao ambiente e melhor qualidade nutritiva.

Este estudo descreve as alteracoes teciduais em individuos de Urochloa brizantha cv. Marandu de populacoes sob acometimento da sindrome da morte das pastagens (SMP). Foram coletados individuos em tres estadios morfologicos distintos, em uma propriedade rural da Amazonia Meridional, no norte de Mato Grosso. Lâminas histologicas foram montadas a partir de seccoes do terco medio de raizes e das folhas, nelas foram avaliadas as proporcoes e caracteristicas dos tecidos em individuos saudaveis e comparadas com individuos em estadio intermediario e estadio mais avancado da SMP. Nas raizes, foi observada diminuicao do espessamento das paredes celulares da exoderme e endoderme, crescente desorganizacao das celulas corticais, lise celular no floema e medula parenquimatica. Nas folhas, ocorreu diminuicao de tricomas, aumento do tamanho das celulas buliformes, diminuicao de fibras e do mesofilo na nervura central. Aparentemente, a SMP provocou as primeiras alteracoes nas raizes, dificultando o fluxo de substâncias e o funcionamento dos tecidos.

True peripheral artery aneurysms proximal to a longstanding arteriovenous fistula is a well-recognized complication. Late aneurysmal degeneration is rare. This study analyzed the characteristics, therapeutic options, and outcomes of true donor brachial artery aneurysms (DBAA) after arteriovenous fistula (AVF) for hemodialysis. We retrospectively collected the data of patients with DBAA after AVF creation, surgically repaired between January 2001 and September 2015. We excluded patients with pseudoaneurysms, anastomotic aneurysms, and infected aneurysms. We recorded patient's demographics, type of access, aneurysm characteristics, symptoms, treatment, and follow-up. Ten patients were treated for aneurysmal degeneration of the brachial artery. Average aneurysm diameter was 37.5 mm. All cases had, at least, one previous distal AVF, ligated or thrombosed, at the time of diagnosis. The first access was created in mean 137 months before the diagnosis of DBAA. Nine patients had previous medical history of renal transplant and were under immunosuppressive therapy. All patients were symptomatic at the time of diagnosis. In all cases, the treatment was aneurysmectomy followed by interposition bypass. One patient developed a postoperative hematoma with the need of surgical drainage. At 50 months of follow-up, one patient was submitted to percutaneous angioplasty due to an anastomotic stenosis. No other complications occurred during the entire follow-up period (mean: 69 months). The pathogenesis underlying DBAA remains unclear. Increased blood flow after AVF creation, immunosuppressive therapy, and ligation/thrombosis of the AVF may contribute to aneurysm formation. Surgical treatment by aneurysmectomy and bypass, with autogenous conducts, is a safe and effective option.

This is among the first studies that associate anatomical characteristics to the environment in the transition region between the two largest phytogeographic domains of South America. We aimed to analyze the leaf anatomy of 22 plant species occurring in the Amazonian savanna in the state of Mato Grosso, Brazil, with the purpose of identifying key characters that show adaptive species strategies in their natural environments. Our hypothesis was that species typical of forest habitats would not present xeromorphic characteristics, while species typical of savanna habitats would present such characteristics. We fixed the samples in FAA 50 , 1 that were stored in alcohol 70%, later they were free-hand cut with a razor blade and stained with astra blue and basic fuchsin. For the analysis of the leaf epidermis, we used the Jeffrey modified method of separation. Characteristics such as thick cuticle, epidermis with thickened wall and straight, presence of silica in the epidermis, tector trichomes, hypostomatic leaves, stomatic crests, dorsiventral mesophyll, presence of collenchyma and vascular bundles were associated with adaptive strategies of the plants to the environment of Amazonian savanna. The anatomic characters presented here are of great importance for the species that live in savanna environments, demonstrating plasticity and contributing to the protection of the leaves to the diverse biotic and abiotic factors to which they are subject, ensuring thus the survival of the species. We demonstrate that plant species structure of Amazonian savanna is most likely shaped by environment determinism, and this fact has implications on how we expect such plants evolve in a climate changing world.

espanolRESUMEN Aspidosperma macrocarpon Mart. (Apocynaceae) es empleada en la medicina popular para el tratamiento de la malaria y el dengue. El objetivo de este trabajo fue caracterizar morfoanatomicamente, histoquimicamente y fitoquimicamente el tronco y la lamina foliar de A. macrocarpon, buscando identificar compuestos secundarios con potencial farmacologico para el tratamiento de ambas enfermedades. Muestras de tallos verdes y hojas de A. macrocarpon fueron recolectados en propiedades particulares en el municipio de Alta Floresta, Mato Grosso, Brasil. Las secciones transversales y paradermicas en los organos fueron obtenidas a mano libre, con ayuda de lamina de acero, doblemente coloreadas y montadas en laminas histologicas. El material fresco fue sometido a pruebas histoquimicas y fitoquimicas para deteccion de saponinas, taninos, alcaloides, flavonoides, glucosidos cardiotonicos, antraquinonas, esteroides y terpenos. A. macrocarpon presenta tricomas glandulares, celulas secretoras y cavidades. Detectamos la presencia de taninos, flavonoides, compuestos fenolicos, glucosidos cardiotonicos y alcaloides, que pueden conferir a esta especie actividad antiplasmodica y propiedades antivirales. El presente estudio fue el primero en identificar aspectos de la composicion histoquimica y fitoquimica de ramas jovenes y hojas de A. macrocarpon que pueden estar asociados al uso popular de esa planta para el tratamiento de la malaria y del dengue en una region de transicion entre los biomas Cerrado y Amazonia, donde la malaria y el dengue ocurren endemicamente. EnglishABSTRACT Aspidosperma macrocarpon Mart. (Apocynaceae) is used in popular medicine to treat malaria and dengue. The aim of the present study was to morphoanatomically, histochemistry and phytochemically characterize A. macrocarpon young branches and leaves, seeking to indicate secondary compounds presenting medicinal potential to treat these diseases. Aspidosperma macrocarpon young branches and leaves were collected in private properties located in municipality of Alta Floresta, Mato Grosso State, Brazil. Sectional and paradermic sections were set freehand using a steelblade, were doubly stained and assembled on histological slides. The fresh material was subjected to histochemical and phytochemical tests for detection of saponins, tannins, alkaloids, flavonoids, cardiotonic glycosides, anthraquinone, steroids and terpenes. Aspidosperma macrocarpon presented glandular trichomes, cavities and secretory cells. We detected the presence of tannins, flavonoid, phenolic compounds, cardiotonic glycosides and alkaloids, which may confer antiplasmodic activity and antiviral properties to the species. As far as it is known, the present study was the first to identify aspects of the histochemical and phytochemical composition of young branches and leaves A. macrocarpon which may be associated with the popular use of this plant to treat malaria and dengue in a transition region between the Cerrado and Amazon biomes, where malaria and dengue occur endemically.