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The historical path from the analysis of morphological adaptation to morphogenetic, but with a new information content: morphogenetic → molecular → supramolecular → ... is increasingly acquiring not only a physico-chemical interpretation, but also the path to ecological biotechnology, aimed at the goals and objectives of the developed protective equipment , which contribute to the development in the understanding of the general patterns that biology faces. In this paper, the features of the biological specificity of morphogenesis and structural stability are considered from the perspective of supramolecular biochemistry of the genetic and proteomic basis of total chromatin. It is noted that in the model system, collection germs of wheat seeds, and in the process of their organ-specific, coordinated-regular growth when switching development subprograms: "donor" spring → winter "donor" → again spring, respectively, positioning occurs, in the time period of growth, core histone (Н3+Н4)'': spring-mesocotyl (42 h) → winter-root system (42 h) → spring-whole highly differentiated embryo (24 h, 30 h). Based on the data obtained, a targeted approach to assessing and predicting the prospects of ecogenetic-biotechnological developments is possible.

The molecular-genetic dynamics of the proteome reorganization of the total chromatin matrix of winter wheat removed from the spring wheat is considered from the perspective of the concept of supramolecular chemistry, which is emerging as an interdisciplinary science. The zones of localization of linker and core histones of nucleosomes in genetic subsystems (mesocotyl, root → coleoptile) are shown. A morphogenetic feature of the localization of the core histone (H3 + H4) ꞌꞌ, which claims to be structural stability under stress conditions, was revealed. Perhaps, through the analysis of the physicochemical points of localization of the interaction of histones with the "linker" and "core" DNA of nucleosomes, an approach can be developed according to R. Tom's terminology: "logical and mathematical schemes of the theory and practice of biological specificity."

Based on the experimental data obtained, the issues of proteomic dynamics of the localization of barrier elements directly between the transcriptionally active and inactive zones of total interphase chromatin, as well as the initiation of replication (origin) at the level of individual sites of signal and stress systems perception from the perspective of epigenetics are discussed. These data will be useful to those who are engaged in the development of logical-mathematical schemes of the theory and practice of biological specificity and can enter the database of the ontology of the growth and development stages of kariogenic plants

A fundamental property of many organisms is the ability to feel, to assess direction of the signal action and respond to the environmental conditions. It is known that chromatin plays a major role in organizing the regulation of gene activity. However, our understanding of how state of the suprastructure organization of chromatin and its proteins reacts not only to changes in the environment, but also on the development of specific signals remains largely unclear. In the course of this work, we have analyzed the result of the various ways of chromatin modifications: the regulatory Arg-X protease-processing and inhibition of protein deacetylation with sodium butyrate. Sodium butyrate causes cell cycle arrest in the G0/G1 phase, and promotes of duration of the transcriptional activity of chromatin. Experiments on molecular-genetic state of the chromatin matrix were carried out at the induction of growth morphogenesis in the physiological period of active water absorption of mature seeds and wheat germs, which were purposefully transformed and formed in different environmental conditions. During focused, long-term transforming of spring wheat Artemovka into winter wheat Mironovskaya 808 and the last of them again into Mironovskaya Spring wheat while stopping of the cell cycle in the G0/G1 phase, mainly occurs the active Arg-X protease-processing at the level of non-histone proteins, and linker histones of suprastructures chromatin. We assume that the regulatory proteolytic processing and prolongation of acetylation of proteins can be interconnected in the regulation of conformational transitions of chromatin at the different levels of its organization: both suprastructures and at the more profound proteomic level of non-histone and histone blocks, and have its peculiarities during the period of transcriptional activation. We hope that the study peculiarities of locations of regulatory proteolysis in the conditions of inhibition of deacetylation in spring and winter forms of wheat can give a new possibility for understanding the epigenetic code of plants in order to increase crop yields and quality.

Investigation morphogenetic processes of how the plant is formed for interact with the environment is necessary to understanding the fundamental problems of stress supramolecular biochemistry of developmental biology. Modern biology has established that the molecular morphogenesis belonging to the number of morphological processes. The molecular morphogenesis is directly based on well-studied forces of intermolecular interactions. From the progress of understanding the morphogenesis, expected not only purely cognitive, but also the practical results of biotechnology. The purpose of work was the analysis of shielding of morphogenetic suprastructures of interphase chromatin matrix to the localization of sensitivity of the regulatory proteolysis in the conditions of adaptation of vegetative phase of wheat to the stressful environmental factors. It was shown that in the conditions of long-term adaptation to cold shock of wheat at the level of suprastructures, of the packaged in the nuclear matrix of the genome, is carried out the total shielding of arginine-X zones for the regulatory protease-processing. Perhaps these zones represents one of the elements of the LCR (locus control regions), which are related to the histone code and influence the architectural organization of the cell nucleus, which is formed to facilitate life of organism in complex environmental conditions. These data will be useful for those who involved in the development of mathematical-logic schemes of theory and practice of biological specificity and may be included in the ontology of stages of plant growth and development.

Research of fundamental molecular and genetic processes of plant interaction with the environment, is a progressive field of understanding the fundamental problems of stress supramolecular biochemistry of developmental biology. The purpose of the work was the analysis of localization shielded to protease processing proteins of suprastructures of interphase chromatin matrix in the conditions of adaptation during vegetative phase of wheat to stressful environment factors. It is shown that in the conditions of perennial adaptation to cold shock of wheat at the level of chromatin suprastructures tightly bound to the nuclear matrix there is a total shielding of arginine-X sites to protease-processing. Perhaps these are zones that affect to the architecture organization of the cell nucleus that can help to survive in complex environmental conditions. According to the priorities in the study of agricultural plants, put forward by EPIC (The Epigenomics of Plants International Consortium) in 2011 for the next decade, was included the point of necessity to understand the molecular basis of the interactions of genotype and environment that change the characteristics of plants in different conditions of the environment. These data will be useful for those who involved in the development of mathematical logic schemes of the theory and practice of biological specificity, and it could be included in the ontology of the stages plant growth and development.

A rapid method is developed for the quantitative determination of 5-35 g/L of isonicotinic hydrazide (INH, isoniazide) in aqueous media. The method is based on the formation of copper(I) oxide in the reaction of isonicotinic hydrazide with copper ions on the surface of indicator tablets and the subsequent recording of the analytical signal by reflectance spectrophotometry and colorimetry. Aluminum oxide is proposed as a solid-phase substrate for the production of indicator tablets. Isonicotinic hydrazide can be determined in the tablet form of the Isoniazid preparation. Keywords: isoniazid, colorimetry, indicator tablets, analysis of pharmaceutical preparations, reflectance spectrophotometry DOI: 10.1134/S1061934818030139, The determination of the concentration of medicinal substances in pharmaceutical products is an important analytical task. A number of studies were devoted to the development of new methods for the [...]