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25 results on '"Itou, Kenji"'

3. Efficient quantification of Globodera pallidaandG. rostochiensis(Tylenchida: Heteroderidae) in large amounts of soil using probe-based real-time PCR

Author

Sakata, Itaru, Itou, Kenji, and Kushida, Atsuhiko

Abstract

Real-time quantitative polymerase chain reaction (qPCR) is used to estimate the population densities of the potato cyst nematodes Globodera pallidaStone and Globodera rostochiensis(Wollenweber) Skarbilovich (Tylenchida: Heteroderidae). Since it is difficult to extract nematode DNA from large amounts of soil (≥ 100 g, enough for quantification of cyst nematodes), cyst isolation is required before DNA extraction. However, when isolating cysts from the soil, various impurities are simultaneously isolated, and separating the cysts from these impurities is laborious. Although previous studies have reported methods for extracting DNA from mixtures of cysts and impurities, it is unclear whether such DNA can be used to estimate nematode densities using qPCR. To examine the effects of impurities on the accuracy of qPCR quantification, we extracted DNA from nematode eggs (G. pallidaand G. rostochiensis) mixed with impurities and performed qPCR. The results suggested that the differences in the fields affected the quantification accuracy. Therefore, field-specific standard curves should be set, which are impractical for routine diagnosis. To propose a more practical method, we determined a fixed standard curve for each species and estimated the population densities in field soil samples by qPCR using the standard curves. The estimated population densities significantly correlated with those determined using conventional microscopic inspections. This study revealed that the population densities of G. pallidaand G. rostochiensiscan be estimated from large amounts of soil, probably only approximately, but efficiently, by qPCR using DNA extracted from mixtures of cysts and impurities.

Published
2024
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7. Effect of KT-611 (Naftopidil) on the contraction of human prostatic tissue and its use in benign prostatic obstruction

Author

Yamanaka, Naoto, Yamaguchi, Osamu, Kameoka, Hiroshi, Fukaya, Yasuo, Yokota, Takashi, Shiraiwa, Yasuo, Yokoyama, Jun, Kumakawa, Kenjirou, Itou, Kenji, Kuma, Yoshinobu, Kobayashi, Masato, and Takaiwa, Masashi

Subjects
arblocker, 494.9, KT-611 (naftopidil), Benign prostatic hypertrophy
Abstract

良性前立腺肥大症49例で1日1或いは2回本剤を投与した所, 自・他覚的症状は有意に改善された.残尿量及び残尿率は有意に減少した.平均及び最大尿流量率は有意に増加した.適正量は, 1日1回, 25～75 mg, 或いは1日2回, 25～100 mgと思われた.有害反応及び異常な臨床検査所見は軽度であった, A new alpha 1-adrenergic receptor antagonist, KT-611 (naftopidil) antagonized dose dependently the contraction induced by agonists, noradrenaline and phenylephrine in the human prostatic tissue. KT-611 at a dosage of once or twice a day was evaluated for its effects on 49 patients with benign prostatic hypertrophy. The drug improved subjective and objective symptoms significantly. The residual urine was reduced in volume and percentage significantly. The average and maximum flow rates increased significantly. The optimal dosage was presumed to be in the range of 25 to 75 mg once a day or 25 to 100 mg twice a day. Adverse reactions and abnormal laboratory findings were all slight. KT-611 was concluded to be useful in the treatment of patients with benign prostatic hypertrophy.

Published
1991

8. Safety and efficacy of oral rehydration therapy until 2 h before surgery: a multicenter randomized controlled trial

Author

Itou, Kenji, primary, Fukuyama, Tatsuya, additional, Sasabuchi, Yusuke, additional, Yasuda, Hiroyuki, additional, Suzuki, Norihito, additional, Hinenoya, Hajime, additional, Kim, Chol, additional, Sanui, Masamitsu, additional, Taniguchi, Hideki, additional, Miyao, Hideki, additional, Seo, Norimasa, additional, Takeuchi, Mamoru, additional, Iwao, Yasuhide, additional, Sakamoto, Atsuhiro, additional, Fujita, Yoshihisa, additional, and Suzuki, Toshiyasu, additional

Published
2011
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Author

Yamanaka, Naoto, Yamaguchi, Osamu, Kameoka, Hiroshi, Fukaya, Yasuo, Yokota, Takashi, Shiraiwa, Yasuo, Yokoyama, Jun, Kumakawa, Kenjirou, Itou, Kenji, Kuma, Yoshinobu, Kobayashi, Masato, Takaiwa, Masashi, Yamanaka, Naoto, Yamaguchi, Osamu, Kameoka, Hiroshi, Fukaya, Yasuo, Yokota, Takashi, Shiraiwa, Yasuo, Yokoyama, Jun, Kumakawa, Kenjirou, Itou, Kenji, Kuma, Yoshinobu, Kobayashi, Masato, and Takaiwa, Masashi

Abstract

A new alpha 1-adrenergic receptor antagonist, KT-611 (naftopidil) antagonized dose dependently the contraction induced by agonists, noradrenaline and phenylephrine in the human prostatic tissue. KT-611 at a dosage of once or twice a day was evaluated for its effects on 49 patients with benign prostatic hypertrophy. The drug improved subjective and objective symptoms significantly. The residual urine was reduced in volume and percentage significantly. The average and maximum flow rates increased significantly. The optimal dosage was presumed to be in the range of 25 to 75 mg once a day or 25 to 100 mg twice a day. Adverse reactions and abnormal laboratory findings were all slight. KT-611 was concluded to be useful in the treatment of patients with benign prostatic hypertrophy.

Published
1991

24. Theoretical study of Al(III)-catalyzed conversion of glyoxal to glycolic acid: dual activated 1,2-hydride shift mechanism by protonated Al(OH)3 species.

Author

Ohshima, Takashi, Yamamoto, Yoshihiro, Takaki, Usaji, Inoue, Yoshihisa, Saeki, Takuya, Itou, Kenji, Maegawa, Yusuke, Iwasaki, Takanori, and Mashima, Kazushi

Subjects
METAL ions, ALUMINUM catalysts, GLYOXAL, GLYCOLIC acid, HYDRIDES, DENSITY functionals, HYDROGEN bonding, ACTIVATION (Chemistry), METALLIC oxides
Abstract

Density functional theory calculations demonstrate that Al(III)-catalyzed conversion of glyoxal to glycolic acid proceeds via a 7-membered dual Lewis acid-hydrogen bonding activation transition state of the 1,2-hydride shift, rather than the previously proposed 5-membered metal-alkoxide chelate activation transition state. [ABSTRACT FROM AUTHOR]

Published
2009
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