Search

Your search keyword '"Itagaki Yasuharu"' showing total 28 results
Start Over Author "Itagaki Yasuharu"
28 results on '"Itagaki Yasuharu"'

13. Anaphylactic reaction to dietary oats

Author

Inuo, Chisato, primary, Kondo, Yasuto, additional, Itagaki, Yasuharu, additional, Kurihara, Kazuyuki, additional, Tsuge, Ikuya, additional, Yoshikawa, Tetsushi, additional, and Urisu, Atsuo, additional

Published
2013
Full Text
View/download PDF

22. Evaluation of Allergenicity of Acid-Hydrolyzed Wheat Protein Using an in vitro Elicitation Test.

Author

Nakamura, Rika, Nakamura, Ryosuke, adachi, Reiko, Itagaki, Yasuharu, Fukutomi, Yuma, and Teshima, Reiko

Subjects
WHEAT, FOOD allergy, GLUTEN, HYDROLYSIS, IMMUNOGLOBULIN E, ALLERGIES, PATIENTS
Abstract

Background: We performed an in vitro elicitation test to de-termine the ability of different types of wheat-allergic pa-tients' IgE to induce humanized mast cell activation after the addition of various time-treated acid-hydrolyzed wheat pro-teins (HWPs). Methods: The reactivity of heat- and various time-treated acid-hydrolyzed glutens (acid-HGs) and com-mercial acid-HWP (HWP1), using serum IgE from wheat al-lergy accompanied by skin and rhinoconjunctlval sensitiza-tion to HWP1 in the facial soap, pediatric subjects with food allergy to native wheat, adult wheat-dependent exercise-in-duced anaphylaxis subjects, and nonatopic healthy subjects, was elucidated by dot blot and a luciferase assay-based in vitro elicitation test (EXiLE test). Results: Serum from sub-jects sensitized with HWP1 reacted only to acid-HGs (acid-HGs treated for 0.5-3 or 6 h), but not native gluten, in the results of the dot blot. In contrast, sera from pediatric sub-jects sensitized with native wheat reacted to native gluten more strongly and showed only slight reactions to 0.5- to 1-hour-treated acid-HGs. The results of the in vitro elicitation test showed that acid hydrolyzation of the gluten attenuat-ed antigen-induced luciferase expression in a time-depen-dent manner for sera from native-wheat-sensitized pediatric subjects. On the other hand, in the sera from HWP1-sensi-tized subjects, acid hydrolyzation of the gluten for 0.5 h dra-matically increased luciferase expression. Conclusions: Even after prolonged hydrolyzation, acid-HGs still retained the ability to activate mast cells in the case of HWP1-sensitized subjects. [ABSTRACT FROM AUTHOR]

Published
2013
Full Text
View/download PDF

28. Yeast mannan in combination with high concentration of Ca2+ enhances production of tissue plasminogen activator by human diploid fibroblasts stimulated with proteose peptone.

Author

Itagaki, Yasuharu

Subjects
YEAST, PROTEOLYTIC enzymes, FIBROBLASTS, PLASMINOGEN, FIBRINOLYTIC agents, ENZYMES, LEAVENING agents
Abstract

The efficient production of tissue plasminogen activator by human diploid fibroblasts is described. Confluent fibroblasts lost their tissue plasminogen activator-producing ability when incubated in a serum-free control medium containing proteose peptone (10 mg ml-1) for longer than 4 to 7 d. In comparison, the cells continued producing the enzyme for more than 14 d when incubated in an experimental medium containing yeast mannan (2 mg ml-1) and CaCl2 (3.6 mM) in addition to peptone. The productivity of the enzyme maximally increased when the ratio of the medium volume to the monolayer area decreased. Such an improved culture system yielded 29 times the productivity achieved under the control conditions. The use of mannan is practically important for large-scale production and subsequent purification of the enzyme. [ABSTRACT FROM AUTHOR]

Published
2002
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results