1. Lipase-catalysed esters synthesis of cafestol and kahweol.
- Author
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Novaes FJM, Itabaiana Junior I, Sutili FK, Marriott PJ, Bizzo HR, Aquino Neto FR, Souza ROMA, and Rezende CM
- Subjects
- Biocatalysis, Chromatography, Gas, Chromatography, High Pressure Liquid, Coffee chemistry, Coffee metabolism, Diterpenes chemistry, Esters analysis, Diterpenes metabolism, Esters metabolism, Lipase metabolism
- Abstract
Cafestol and kahweol (C&K), two coffee diterpene alcohols with structural similarity which exhibit anticarcinogenic effects, were isolated from green coffee Arabica beans, followed by their lipase-catalysed esterification and purification by preparative high-performance liquid chromatography (HPLC). The isolation and enzymatic synthesis parameters of C&K esters were studied, with the latter optimised by a Central Composite Design; both procedures were monitored by gas chromatography. Scale up and improved isolation conditions resulted in 1.29 g of C&K, with 98% purity from 300 g of green Arabica beans. The highest C&K ester yields were observed using an alcohol:fatty acid molar ratio of 1:5, 73.3 mg mL
-1 of CAL-B enzyme, 70 °C and 240 rpm for 3 days in toluene, leading to 85-88% conversion among a variety of tested C&K esters, including n-C14:0 -C20:0 , C18:1 , C18:2 and C18:3 ., (Copyright © 2018. Published by Elsevier Ltd.)- Published
- 2018
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