21 results on '"Issarawan Keadkraichaiwat"'
Search Results
2. A Longitudinal Study of the Relationship of Adiponectin with Reproduction in Infertile Women Undergoing IVF/ICSI Treatment, and an Experimental Study in Human Granulosa Cells
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Lixian Qin, Chantacha Sitticharoon, Somsin Petyim, Issarawan Keadkraichaiwat, Rungnapa Sririwichitchai, Pailin Maikaew, and Malika Churintaraphan
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adiponectin ,IVF/ICSI treatment ,granulosa cells ,aromatase ,FSH ,Science - Abstract
This study investigated the roles of adiponectin in IVF treatment during Phase I (the basal stage before gonadotropin administration), Phase II (approximately 8 days after gonadotropin administration), and Phase III (on the ovum pick-up day), as well as the effects of adiponectin on CYP19A1 and the FSH receptor (FSHR) mRNA expression in a human granulosa-like tumor cell line (KGN). In human subjects (a longitudinal study, n = 30), blood samples were collected in all phases, while follicular fluid (FF) was only collected in Phase III. The participants were classified into successful and unsuccessful groups based on the determination of fetal heartbeats. KGN cells were treated with adiponectin/FSH/IGF-1 (an experimental study, n = 3). There was no difference in the adiponectin levels between successful and unsuccessful pregnancies in the FF (Phase III) and in serum (all phases), as well as among the three phases in both groups. Serum FSH (Phase I) was positively associated with serum adiponectin in the unsuccessful group, but it had a negative association in the successful group (all phases). Serum adiponectin and serum FSH (Phase I) were positively correlated in the unsuccessful group, whereas they were negatively correlated (all phases) in the successful group. The serum adiponectin levels (Phase III) were significantly higher than in the FF in unsuccessful pregnancies, but there was no difference in successful pregnancies. FF adiponectin concentrations were negatively correlated with serum LH in successful subjects. In KGN cells, adiponectin had no influence on CYP19A1 and FSHR mRNA expression. High adiponectin levels in serum compared to FF (Phase III) in unsuccessful subjects might negatively impact IVF treatment.
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- 2023
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3. Cissus Quadrangularis enhances UCP1 mRNA, indicative of white adipocyte browning and decreases central obesity in humans in a randomized trial
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Saimai Chatree, Chantacha Sitticharoon, Pailin Maikaew, Kitchaya Pongwattanapakin, Issarawan Keadkraichaiwat, Malika Churintaraphan, Chanakarn Sripong, Rungnapa Sririwichitchai, and Sompol Tapechum
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Medicine ,Science - Abstract
Abstract Obesity is associated with the growth and expansion of adipocytes which could be decreased via several mechanisms. Cissus Quadrangularis (CQ) extract has been shown to reduce obesity in humans; however, its effect on human white adipocytes (hWA) has not been elucidated. This study aimed to investigate the effects of CQ on obesity, lipolysis, and browning of hWA. CQ treatment in obese humans significantly decreased waist circumference at week 4 and week 8 when compared with the baseline values (p
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- 2021
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4. Serum Adiponectin, Visfatin, and Omentin Compared between Non-pregnant and Pregnant Women in Overall, Non-obese, and Obese subjects
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Chantacha Sitticharoon, M.D., Ph.D., Xaynaly Souvannavong-Vilivong, M.Sc., Roongrit Klinjampa, M.Sc., Malika Churintaraphan, M.Sc., Nay Chi Nway, MBBS., M.Sc., Issarawan Keadkraichaiwat, B.Sc., and Tripop Lertbunnaphong, M.D.
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Adiponectin ,insulin resistance ,omentin ,pregnancy ,visfatin ,(Siriraj Med J 2018 ,70: 219-226) ,Medicine - Abstract
Objective: This study aimed to compare serum adiponectin, visfatin, and omentin between non-pregnant and pregnant women in overall, non-obese, and obese subjects. Methods: There were 40 pregnant and 33 non-pregnant women classified by body mass index (BMI) into non-obese or obese subjects. Fasting blood samples were collected in the morning for the non-pregnant group and before delivery for the pregnant group. Results: Plasma glucose levels were significantly lower, but plasma insulin levels were significantly higher in pregnant when compared to non-pregnant women in overall, non-obese, and obese women (p
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- 2018
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5. ODP417 Kisspeptin directly stimulates estrogen and progesterone secretion from human granulosa cells
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Chantacha Sitticharoon, Rungnapa Sririwichitchai, Issarawan Keadkraichaiwat, Pailin Maikaew, and Malika Churintaraphan
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Endocrinology, Diabetes and Metabolism - Abstract
Kisspeptin is a peptide hormone that has a central role to stimulate gonadotropin-releasing hormone secretion from the hypothalamus. Furthermore, it was shown to have peripheral roles on enhancing CYP19 (aromatase) expression and aromatase secretion from human granulosa cells (1). The objectives of this study were to determine direct effects of kisspeptin treatment on estrogen and progesterone secretion from human granulosa-like tumor cell line (KGN). KGN cells (n=3) were treated with 10 nM follicle stimulating hormone (FSH); 100, 1,000, and 10,000 nM kisspeptin; and FSH together with different doses of kisspeptin for 24 hours. Estrogen and progesterone concentrations in supernatant were measured by enzyme immunoassay kits. The results showed that FSH treatment alone (P Presentation: No date and time listed
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- 2022
6. Roles of kisspeptin in IVF/ICSI-treated infertile women and in human granulosa cells
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Rungnapa Sririwichitchai, Chantacha Sitticharoon, Somsin Petyim, Chanakarn Sripong, Lixian Qin, Malika Churintaraphan, Pailin Maikeaw, and Issarawan Keadkraichaiwat
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Adult ,Infertility ,endocrine system ,media_common.quotation_subject ,medicine.medical_treatment ,Regulator ,030209 endocrinology & metabolism ,Biology ,General Biochemistry, Genetics and Molecular Biology ,Andrology ,03 medical and health sciences ,Aromatase ,0302 clinical medicine ,Kisspeptin ,medicine ,Humans ,Sperm Injections, Intracytoplasmic ,Insulin-Like Growth Factor I ,Original Research ,media_common ,Kisspeptins ,Granulosa Cells ,030219 obstetrics & reproductive medicine ,In vitro fertilisation ,Ivf icsi ,medicine.disease ,Follicular Fluid ,Female ,Reproduction ,Infertility, Female ,hormones, hormone substitutes, and hormone antagonists - Abstract
Kisspeptin, a crucial central regulator of reproduction, has been used as a trigger in in vitro fertilization (IVF) treatment. This study aimed to investigate the roles of kisspeptin in IVF treatment in infertile females ( n = 30); and in steroidogenesis in human granulosa-like tumor cell line (KGN). In the human study, blood was collected at three time points including (1) the beginning of gonadotropin stimulation (Phase I), (2) around eight days after gonadotropin stimulation (Phase II), and (3) on the day of ovum pick-up (Phase III). Follicular fluid (FF) was collected at Phase III. Serum human chorionic gonadotropin (hCG) was measured 15 days after embryo transfer and fetal heart beats were determined around 42 days of menstrual cycle to classify the subjects into successful and unsuccessful groups. FF kisspeptin levels were higher in successful compared with unsuccessful subjects ( P -2 mmol/L with FSH enhanced aromatase concentrations in the supernatant compared with FSH alone ( P
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- 2020
7. Placental expressions and serum levels of adiponectin, visfatin, and omentin in GDM
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Xaynaly Souvannavong-Vilivong, Rungnapa Sririwichitchai, Roongrit Klinjampa, Tripop Lertbunnaphong, Saimai Chatree, Chantacha Sitticharoon, Chanakarn Sripong, and Issarawan Keadkraichaiwat
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Adult ,medicine.medical_specialty ,Placenta ,Endocrinology, Diabetes and Metabolism ,medicine.medical_treatment ,Gene Expression ,030209 endocrinology & metabolism ,030204 cardiovascular system & hematology ,GPI-Linked Proteins ,Body Mass Index ,03 medical and health sciences ,0302 clinical medicine ,Endocrinology ,Insulin resistance ,Pregnancy ,Lectins ,Internal medicine ,Diabetes mellitus ,Internal Medicine ,medicine ,Humans ,Insulin ,Nicotinamide Phosphoribosyltransferase ,Adiponectin ,business.industry ,nutritional and metabolic diseases ,Gestational age ,General Medicine ,medicine.disease ,Gestational diabetes ,Diabetes, Gestational ,Case-Control Studies ,Cytokines ,Female ,Insulin Resistance ,business ,Body mass index - Abstract
Adiponectin, visfatin, and omentin have been shown to be associated with insulin sensitivity and might have a role in the pathophysiology of gestational diabetes mellitus (GDM). This study aimed to (1) compare adiponectin, visfatin, and omentin mRNA expressions in placenta and their serum levels between normal pregnancy (NP) and GDM class A1 (GDMA1) pregnancy and (2) determine correlations between placental gene expressions as well as serum levels with maternal and neonatal clinical parameters in all, NP, and GDM subjects. NP subjects (n = 37), who had normal medical history during their pregnancies without diagnosis of any abnormalities and GDMA1 subjects (n = 37), who were diagnosed since they had antenatal care, were recruited when they were in labor with a gestational age of at least 34 weeks. Clinical parameters and serum adiponectin, visfatin, and omentin levels were measured in the delivery room. GDMA1 subjects had higher serum visfatin and plasma glucose levels, but lower serum omentin levels (p
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- 2019
8. Placental Neuropeptide Y (NPY) and NPY receptors expressions and serum NPY levels in preeclampsia
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Saimai Chatree, Tripop Lertbunnaphong, Chantacha Sitticharoon, Roongrit Klinjampa, Issarawan Keadkraichaiwat, Xaynaly Souvannavong-Vilivong, Chanakarn Sripong, and Malika Churintaraphan
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Adult ,medicine.medical_specialty ,Placenta ,030204 cardiovascular system & hematology ,General Biochemistry, Genetics and Molecular Biology ,Preeclampsia ,03 medical and health sciences ,0302 clinical medicine ,Pre-Eclampsia ,Pregnancy ,Internal medicine ,mental disorders ,Gene expression ,medicine ,Humans ,Neuropeptide Y ,Receptor ,Original Research ,Chemistry ,Neuropeptide Y receptor ,medicine.disease ,humanities ,Receptors, Neuropeptide Y ,Endocrinology ,Female ,030217 neurology & neurosurgery - Abstract
Neuropeptide Y (NPY) has been reported as a vasoconstrictive substance that might be associated with preeclampsia. NPY mediates different effects via its specific NPY receptors. NPY action via Y1 receptor (Y1R) and/or Y5 receptor (Y5R) induces vascular smooth muscle cells proliferation while it is implicated in angiogenesis via Y2 receptor (Y2R) and/or Y5R. The objectives of this study were to (1) compare placental NPY, Y1 receptor ( Y1R), Y2 receptor ( Y2R), and Y5 receptor ( Y5R) expressions between normal (NP) and preeclamptic (PE) pregnancies to determine whether gene expression of different NPY receptors are altered in the PE condition; (2) compare maternal serum NPY levels between NP and PE subjects; and (3) determine correlations between placental gene expressions as well as serum NPY levels with maternal and neonatal clinical parameters. There were 22 subjects each in the NP (gestational age 37–42 weeks) and PE (gestational age ≥34 weeks) groups. Clinical parameters and serum NPY levels were measured before delivery. NPY expression and serum NPY levels were comparable between NP and PE subjects. Y1R, Y2R, and Y5R expressions were significantly lower in PE than NP subjects. In all and NP subjects, placental Y2R showed the highest expression, tended to be higher than Y5R, and was significantly higher than Y1R. In PE subjects, placental Y2R was comparable to Y5R and both Y2R and Y5R were significantly higher than Y1R. The NPY receptor expression ratio between the PE/NP groups showed that it was lowest for Y2R (0.27) compared to Y1R (0.42) and Y5R (0.40) suggestive of decreased Y2R expression in PE subjects. In summary, a decrease in placental Y2R mRNA might be associated with abnormalities of placental angiogenesis which probably contributes to the pathophysiology of preeclampsia. The roles of NPY receptors mediating placental vascularization need to be further investigated. Impact statement Neuropeptide Y (NPY) has been reported as a vasoconstrictive substance which might be associated with preeclampsia. The novel findings of this study were that Y1R, Y2R, and Y5R expressions were significantly lower in the PE than the NP group. Moreover, the NPY receptor expression ratio between the PE/NP groups was lowest for Y2R (0.27) compared to Y1R (0.42) and Y5R (0.40) suggestive of a reduction of this receptor in the preeclampsia group. Our results suggested that decreased Y2R mRNA in the PE group might be associated with abnormalities of placental angiogenesis which probably contributes to the pathophysiology of preeclampsia.
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- 2019
9. Cissus Quadrangularis enhances UCP1 mRNA, indicative of white adipocyte browning and decreases central obesity in humans in a randomized trial
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Sompol Tapechum, Chanakarn Sripong, Malika Churintaraphan, Kitchaya Pongwattanapakin, Pailin Maikaew, Rungnapa Sririwichitchai, Saimai Chatree, Issarawan Keadkraichaiwat, and Chantacha Sitticharoon
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0301 basic medicine ,Agonist ,Adult ,Leptin ,Male ,medicine.medical_specialty ,Waist ,medicine.drug_class ,Science ,Lipolysis ,Adipocytes, White ,030209 endocrinology & metabolism ,Article ,law.invention ,03 medical and health sciences ,0302 clinical medicine ,Endocrinology ,Medical research ,Randomized controlled trial ,law ,Internal medicine ,medicine ,Browning ,Cissus quadrangularis ,Humans ,RNA, Messenger ,Uncoupling Protein 1 ,Multidisciplinary ,biology ,business.industry ,Cissus ,Plant Extracts ,Endocrine system and metabolic diseases ,biology.organism_classification ,medicine.disease ,Obesity ,Hormones ,030104 developmental biology ,Adipocytes, Brown ,Obesity, Abdominal ,Medicine ,Female ,business ,Pioglitazone ,medicine.drug - Abstract
Obesity is associated with the growth and expansion of adipocytes which could be decreased via several mechanisms. Cissus Quadrangularis (CQ) extract has been shown to reduce obesity in humans; however, its effect on human white adipocytes (hWA) has not been elucidated. This study aimed to investigate the effects of CQ on obesity, lipolysis, and browning of hWA. CQ treatment in obese humans significantly decreased waist circumference at week 4 and week 8 when compared with the baseline values (p p p UCP1 mRNA when compared to vehicle (p UCP1 expression in a dose-dependent manner when compared to pioglitazone treatment (p UCP1 mRNA in hWA suggestive of its action via browning of hWA.
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- 2021
10. Epigallocatechin gallate decreases plasma triglyceride, blood pressure, and serum kisspeptin in obese human subjects
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Kitchaya Pongwattanapakin, Sompol Tapechum, Rungnapa Sririwichitchai, Issarawan Keadkraichaiwat, Chantacha Sitticharoon, Saimai Chatree, Chanakarn Sripong, Malika Churintaraphan, and Pailin Maikaew
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0301 basic medicine ,Adult ,Blood Glucose ,Leptin ,medicine.medical_specialty ,Lipolysis ,Adipocytes, White ,030209 endocrinology & metabolism ,Blood Pressure ,Epigallocatechin gallate ,Kidney ,complex mixtures ,General Biochemistry, Genetics and Molecular Biology ,Catechin ,Type ii diabetes ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,Kisspeptin ,Plasma triglyceride ,Internal medicine ,medicine ,Humans ,heterocyclic compounds ,Obesity ,RNA, Messenger ,Triglycerides ,Uncoupling Protein 1 ,Original Research ,Kisspeptins ,business.industry ,food and beverages ,Middle Aged ,medicine.disease ,030104 developmental biology ,Endocrinology ,Blood pressure ,Adipocytes, Brown ,chemistry ,Liver ,sense organs ,Adiponectin ,business - Abstract
Obesity is one of major risk factors increasing chronic diseases including type II diabetes, cardiovascular diseases, and hypertension. The effects of epigallocatechin gallate (EGCG), the major active compound in green tea, on reduced obesity and improved metabolic profiles are still controversial. Furthermore, the effects of EGCG on human adipocyte lipolysis and browning of white adipocytes have not been elucidated. This study aimed to investigate the effects of EGCG on obesity, lipolysis, and browning of human white adipocytes. The results showed that, when compared to the baseline values, EGCG significantly decreased fasting plasma triglyceride levels ( P
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- 2020
11. Associations of serum kisspeptin levels with metabolic and reproductive parameters in men
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Rungnapa Sririwichitchai, Malika Churintaraphan, Pornthira Mutirangura, Pailin Maikaew, Namtip Triyasunant, Issarawan Keadkraichaiwat, Arissara Iamaroon, Chantacha Sitticharoon, and Thitima Chinachoti
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Adult ,Leptin ,Male ,medicine.medical_specialty ,Physiology ,030209 endocrinology & metabolism ,Subgroup analysis ,Overweight ,Biochemistry ,Spinal Puncture ,Body Mass Index ,03 medical and health sciences ,Cellular and Molecular Neuroscience ,0302 clinical medicine ,Endocrinology ,Cerebrospinal fluid ,Kisspeptin ,Internal medicine ,medicine ,Humans ,Anesthesia ,Obesity ,Kisspeptins ,medicine.diagnostic_test ,business.industry ,Lumbar puncture ,Reproduction ,Body Weight ,medicine.disease ,Blood pressure ,Female ,medicine.symptom ,Insulin Resistance ,business ,Body mass index ,hormones, hormone substitutes, and hormone antagonists ,030217 neurology & neurosurgery - Abstract
Central kisspeptin action is well known in reproductive regulation; however, its peripheral action is not well understood. This study aimed to 1) compare serum or cerebrospinal fluid (CSF) kisspeptin levels between different body mass index (BMI) groups 2) compare the levels of kisspeptin between serum and CSF, and 3) determine correlations between serum or CSF kisspeptin levels with clinical, metabolic, and reproductive parameters. There were 40 male subjects undergoing operations with lumbar puncture anesthesia. Subgroup analysis was performed to compare between the normal (n = 12), overweight (n = 10), and obese groups (n = 17). One lean subject was recruited for correlation analysis. Serum kisspeptin levels were significantly higher in the obese group when compared to the normal weight and overweight groups even after adjusting for age or diastolic blood pressure (DBP) (p0.05 all). Serum leptin levels were significantly higher in the obese group when compared to the normal weight and overweight groups (p0.05 all). CSF kisspeptin levels were below the minimum detectable concentration for the assay (0.06 ng/mL). Serum kisspeptin was positively correlated with body weight, BMI, plasma insulin, the homeostatic model assessment for insulin resistance (HOMA-IR), and serum leptin but was negatively correlated with plasma LH (p0.05 all). In conclusion, serum kisspeptin was related to obesity, leptin, insulin, and insulin resistance, while CSF kisspeptin was below the limits of detection. Thus, peripheral kisspeptin might have a role in metabolic regulation.
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- 2020
12. SAT-019 The Associations of Kisspeptin with Reproductive Hormones and Oocyte Maturation in Infertile Patients Who Underwent IVF Treatment
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Somsin Petyim, Rungnapa Sririwichitchai, Issarawan Keadkraichaiwat, Chanakarn Sripong, Pailin Maikaew, Chantacha Sitticharoon, Lixian Qin, and Malika Churintaraphan
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Ivf treatment ,business.industry ,Endocrinology, Diabetes and Metabolism ,Reproductive hormones ,Oocyte ,Andrology ,medicine.anatomical_structure ,Kisspeptin ,medicine ,Reproductive Endocrinology ,Clinical Studies in Female Reproduction I ,business ,hormones, hormone substitutes, and hormone antagonists ,AcademicSubjects/MED00250 - Abstract
Kisspeptin has been recognized as a stimulatory factor of the hypothalamic-pituitary-gonadal (HPG) axis. Kisspeptin has been successfully used to trigger ovulation in in vitro fertilization (IVF) treatment (1). This study aimed to 1)compare reproductive hormones (FSH, LH, estradiol, progesterone, and AMH), serum and follicular fluid kisspeptin between successful and unsuccessful clinical pregnancy in different stages of IVF treatment; 2) determine the correlations of serum or follicular fluid kisspeptin with reproductive hormones as well as biochemical and clinical outcomes; and 3) compare the levels of kisspeptin between serum and follicular fluid. In this study, 30 infertile female patients (age 26–40 years old) undergoing IVF treatment were recruited. Blood samples were collected at 3 different time points, the beginning of gonadotropin stimulation (the early follicular phase or T1), the scheduled day for ovum pick-up (OPU) (the late follicular phase or T2), and the day of OPU (T3). Meanwhile, biochemical and clinical parameters including the number of retrieved oocytes, matured oocytes, 2 pronuclear (2PN), and serum hCG were also determined. Moreover, fetus heart beat at 6 weeks of gestation was measured to classify the subjects into successful (n=10) and unsuccessful (n=20) clinical pregnancies. Serum AMH at T1 was significantly higher but serum FSH at T1 and T2 was significantly lower in the success group compared with the unsuccess group (P Thus, kisspeptin might be used as a potential predictor of successful IVF treatment. Reference: (1) Abbara A, et al. J Clin Endocrinol Metab. 2015;100(9):3322–31.
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- 2020
13. MON-012 The Direct Effect of Kisspeptin on Human Ovarian Granulosa Cells to Regulate Steroidogenesis
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Pailin Maikaew, Rungnapa Sririwichitchai, Malika Churintaraphan, Issarawan Keadkraichaiwat, Chantacha Sitticharoon, and Lixian Qin
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endocrine system ,Kisspeptin ,Endocrinology, Diabetes and Metabolism ,Reproductive Endocrinology ,Biology ,Female Reproduction: Basic Mechanisms ,hormones, hormone substitutes, and hormone antagonists ,AcademicSubjects/MED00250 ,Cell biology - Abstract
Kisspeptin has a central role to stimulate the hypothalamic-pituitary-gonadal (HPG) axis. Furthermore, a previous study has suggested that kisspeptin might have a peripheral role in follicular development (1). This study aimed to 1) explore the effect of kisspeptin on CYP19A1 (aromatase) mRNA expression in human granulosa cells and aromatase concentrations in the supernatant; and 2) investigate the effect of kisspeptin on FSHR mRNA expression in human granulosa cells. In this study, human granulosa-like tumor cell line (KGN) (n=3) was incubated for 24 hours with FSH (10-8 M); FSH with IGF-1 (10-8 M); different doses of kisspeptin including 1, 10, 100, 1,000, and 10,000 nM; FSH with different doses of kisspeptin; and FSH with IGF-1 together with different doses of kisspeptin. FSH treatment alone or FSH with IGF-1 did not increase CYP19A1 mRNA expression when compared to control. Interestingly, kisspeptin treatment at the doses of 100 nM (P=0.028), 1,000 nM (P=0.005), and 10,000 nM (P=0.009) in the presence of FSH together with IGF-1 enhanced CYP19A1 mRNA expression when compared with control. Furthermore, FSH or FSH with IGF-1 or FSH with all doses of kisspeptin or FSH with IGF-1 together with all doses of kisspeptin increased aromatase concentrations in the supernatant when compared to control (P
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- 2020
14. SAT-658 Associations of Serum and CSF Kisspeptin Levels with Metabolic and Reproductive Parameters in Men
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Pailin Maikaew, Arissara Iamaroon, Malika Churintaraphan, Chantacha Sitticharoon, Namtip Triyasunant, Thitima Chinachoti, Pornthira Mutirangura, and Issarawan Keadkraichaiwat
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medicine.medical_specialty ,Endocrinology ,Kisspeptin ,Lipids, Obesity and Metabolic Disease ,Endocrinology, Diabetes and Metabolism ,Internal medicine ,medicine ,Biology ,Diabetes Mellitus and Glucose Metabolism ,AcademicSubjects/MED00250 ,hormones, hormone substitutes, and hormone antagonists - Abstract
Action of kisspeptin in the central nervous system (CNS) is well known on reproductive regulation; however, its peripheral action is not well understood. Recent studies have shown that peripheral kisspeptin might be related to obesity and/or metabolic parameters in humans [1]; however, these associations are still inconclusive. This study aimed to 1) compare serum or cerebrospinal fluid (CSF) kisspeptin levels between different body mass index (BMI) groups 2) compare levels of kisspeptin between serum and CSF, and 3) determine correlations between serum or CSF kisspeptin levels with clinical, metabolic, and reproductive parameters. There were 40 male subjects who underwent an operation with lumbar puncture anesthesia. Subgroup analysis was performed to compare between the lean-normal group (n=13) which included lean and normal weight subjects, the overweight group (n=10), and the obese group (n=17) according to BMI. Blood samples were collected after at least 8-hour fasting before intravenous cannulation prior to the operation while CSF samples were obtained by lumbar puncture before administration of the spinal anesthesia. Serum kisspeptin and leptin levels were significantly higher in the obese group when compared to the lean-normal and overweight groups even after adjusted to age while CSF kisspeptin levels were comparable between different BMI groups (p
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- 2020
15. Happiness of Preclinical Students: Evaluating Factors Influencing Happiness of Preclinical Students
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Vasu Lertsiripatarajit, Pailin Maikaew, Issarawan Keadkraichaiwat, Nipith Charoenngam, and Chantacha Sitticharoon
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education - Abstract
This study aimed to determine factors influencing happiness in preclinical students especially in aspects of teaching and learning, students’ motivation, study habits, and academic achievement. This study was a questionnaire-based cross-sectional study. The questionnaire was validated and approved for clarity, readability, rational analysis, and comprehensiveness by the experts. Questionnaires were sent to all second and/or third-year medical students of the 2018 (328) and 2019 (329) classes, Faculty of Medicine Siriraj Hospital, Mahidol University, Thailand. Cronbach’s alpha values were 0.886. The data were analyzed by the Statistical Package for Social Science version 18. From open-ended questions, the top 5 ranking factors increasing students’ happiness were teaching of instructors, content satisfaction, content understanding, free time, and handouts. Factors that had positive influences/associations with happiness of preclinical students were happiness in learning subjects taught in a particular year; satisfaction of content, study outcomes, materials, handouts, teaching motivating desire to learn, and applicability of content to medical profession; high motivation to study medicine, expected score, and the percentage of achievement of study targets; less amount of time spent on recorded-e-lecture study and internet for non-academic use; and low stress. Happiness of preclinical students was influenced by satisfaction in teaching and learning, their attitude, study habits, and academic achievement. Further studies on determining students’ happiness after improvement of teaching and learning environments, augmenting activities that enhance students’ motivation, and promoting good study habits among medical students, are required to prove whether enhancement of these factors could effectively increase students' happiness.
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- 2021
16. The Effects of Adiponectin on Infertile Women Undergoing IVF/ICSI Treatment and on Human Granulosa Cells
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Somsin Petyim, Malika Churintaraphan, Pailin Maikeaw, Issarawan Keadkraichaiwat, Chantacha Sitticharoon, Rungnapa Sririwhitchai, and Lixian Qin
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Andrology ,Ovary, Testes, and Impact of Hormones on Metabolic Function ,Adiponectin ,business.industry ,Endocrinology, Diabetes and Metabolism ,Medicine ,Reproductive Endocrinology ,Ivf icsi ,business ,hormones, hormone substitutes, and hormone antagonists ,AcademicSubjects/MED00250 - Abstract
Adiponectin, one of the most abundant adipocyte-secreted protein, has been involved in female reproductive regulation. This study aimed to 1) compare serum adiponectin levels in various phases of in vitro fertilization (IVF)/intracytoplasmic sperm injection (ICSI) treatment including Phase I (the beginning of gonadotropin stimulation), Phase II (around 8 days after gonadotropin stimulation), and Phase III (on the day of ovum pick-up) between success and unsuccess subjects; 2) compare follicular fluid (FF) adiponectin levels between success and unsuccess groups; 3) compare serum adiponectin levels among different phases of IVF/ICSI treatment in success and unsuccess groups; 4) compare the levels of adiponectin between serum and FF; and 5) investigate the effects of adiponectin on mRNA expressions of follicle stimulating hormone receptor (FSHR) and CYP19A1 (aromatase) in human granulosa-like tumor cell line (KGN) (n=3). In the human study, recruited participants (n=30) with age of 26-40 years were enrolled between April 2018 - May 2019. Blood samples were collected at Phases I, II, and III while FF samples were collected at Phase III. Adiponectin levels were comparable between success and unsuccess subjects in both serum (all phases) and FF (Phase III). Furthermore, serum adiponectin levels were comparable among Phase I, II, and III in success and unsuccess groups. In Phase III, serum adiponectin showed positive correlations with serum adiponectin in Phase I and II and serum FSH in Phase I. Interestingly, adiponectin levels in FF were significantly lower than serum at Phase III in unsuccessful pregnancies but were comparable in successful pregnancies. Moreover, FF adiponectin had a negative correlation with serum LH at Phase III in success subjects. In the KGN cell study, adiponectin had no effects on FSHR and CYP19A1 (aromatase) mRNA expression compared with control. In conclusion, high adiponectin levels in serum compared to FF might contribute to unsuccessful IVF/ICSI treatment.
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- 2021
17. Factors influencing the National License Examination step 1 score in preclinical medical students
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Issarawan Keadkraichaiwat, Pongtawat Lertwilaiwittaya, Chantacha Sitticharoon, and Pailin Maikaew
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Male ,Students, Medical ,020205 medical informatics ,Physiology ,02 engineering and technology ,Academic achievement ,Education ,03 medical and health sciences ,Young Adult ,0302 clinical medicine ,0202 electrical engineering, electronic engineering, information engineering ,medicine ,Humans ,030212 general & internal medicine ,License ,Medical education ,business.industry ,Attendance ,General Medicine ,Licensure, Medical ,Thailand ,Anxiety ,The Internet ,Female ,Educational Measurement ,medicine.symptom ,Psychology ,business ,Education, Medical, Undergraduate - Abstract
The National License Examination step 1 (NLE1), which tests basic medical sciences knowledge in Thailand, is considered to be tough and stressful for medical students due to the large amount of content. This study aimed to determine factors influencing the NLE1 score (NLE1S). The NLE1S, academic achievement, and class attendance were obtained officially. Other factors, including study habits, were obtained via a questionnaire, with 81.97% (241/294) being returned. Students were divided into four groups according to the central passing score and Z-score of the NLE1S, including the fail (
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- 2019
18. Impact of an early clinical exposure project conducted by senior clinical students compared between participating and nonparticipating students
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Chantacha Sitticharoon, Rungnirand Praditsuwan, Thanapat Vanichnatee, Nipith Charoenngam, Punyapat Maprapho, and Issarawan Keadkraichaiwat
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Male ,Medical education ,Motivation ,Academic Success ,Students, Medical ,020205 medical informatics ,Education, Medical ,Physiology ,education ,02 engineering and technology ,General Medicine ,Education ,03 medical and health sciences ,Young Adult ,0302 clinical medicine ,Surveys and Questionnaires ,0202 electrical engineering, electronic engineering, information engineering ,Humans ,Female ,030212 general & internal medicine ,Clinical Competence ,Psychology ,Retrospective Studies - Abstract
An early clinical exposure project conducted by clinical students aimed to promote direct clinical experience to preclinical students. The aim of this study was to determine the effects of the project on academic achievement and study attitudes and habits between participating and nonparticipating students before ( test 1) and after the project ( test 2) in the second preclinical year and at the end of the first semester of the first clinical year ( test 3), with a subgroup analysis of the first (lowest) to third (highest) tertile of the score. Questionnaires were sent to the first clinical year students at test 3 and asked the information retrospectively at test 1 and test 2 in second year preclinical and currently at test 3, with 83.86% (265/316) being returned. Mean percentile of scores was higher at test 2 compared with test 1 in the first tertile group of participating students. Motivation to study medicine (motivation), realization of application of preclinical knowledge to clinical study (application), understanding of clinical environment (environment), and lesson review after class (review) were higher for participating than nonparticipating students at test 2 and/or test 3. Searching additional study information was higher at test 2 compared with test 1 only for participating students. This project could effectively promote application, motivation, environment, and review for participating vs. nonparticipating students at test 2 and/or test 3. Effortless, intimate, and effective communication between clinical and preclinical students and a direct experience in early clinical exposure might be key success factors.
- Published
- 2018
19. Factors Influencing Academic Achievement in Preclinical Students
- Author
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Issarawan Keadkraichaiwat, Pailin Maikaew, Nipith Charoenngam, Chantacha Sitticharoon, and Yanint Raksadawan
- Subjects
education - Abstract
Academic achievement is a major concern of preclinical students. This study aimed to determine factors influencing academic achievement in preclinical years. Questionnaires were sent to all students of the 2019 class at the 1st (2019/1) and 2nd preclinical years (2019/2) and the 2018 class at the 2nd preclinical year (2018/2) without sampling, with 85.11% (280/328), 86.32% (284/328), and 83.54% (274/328) being returned, respectively. Students were divided into Q1 (lowest)-Q2-Q3-Q4 (highest) according to quartiles of their GPA. Q4 students had higher examination expectation and achievement of study targets in all classes and more time spent on non-recorded-e-lecture study in the 2018/2 and 2019/2 classes; but lower time spent on recorded-e-lecture study in the 2019/1 class and non-academic-internet use in the 2019/2 and 2018/2 classes than other groups. In contrast, Q1 students had higher instances of lateness and absence/year in the 2019/2 and 2018/2 classes but lower happiness scores in the 2018/2 class compared to other groups. Obese and overweight students had significantly lower GPA than normal weight and underweight students of the 2019/2 class (p
- Published
- 2020
20. Factors influencing the National License Examination step 1 score in preclinical medical students.
- Author
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Pongtawat Lertwilaiwittaya, Chantacha Sitticharoon, Pailin Maikaew, and Issarawan Keadkraichaiwat
- Subjects
NATIONAL competency-based educational tests ,MEDICAL students ,MEDICAL sciences ,ACADEMIC motivation ,ACADEMIC achievement - Abstract
The National License Examination step 1 (NLE1), which tests basic medical sciences knowledge in Thailand, is considered to be tough and stressful for medical students due to the large amount of content. This study aimed to determine factors influencing the NLE1 score (NLE1S). The NLE1S, academic achievement, and class attendance were obtained officially. Other factors, including study habits, were obtained via a questionnaire, with 81.97% (241/294) being returned. Students were divided into four groups according to the central passing score and Z-score of the NLE1S, including the fail (<52%; n = 13), low-pass (52 to <70%; n = 121), high-pass (70 to <80%; n = 89), and excellent (≥80%; n = 18) groups. Men had higher NLE1S (P < 0.001) and comprehensive examination scores (P < 0.001) than women. Students with high motivation to study medicine had higher NLE1S. Daily preparation time (h/day) was lower, but stress was higher, in the fail group. In the excellent group, internet for academic use and achievement of study targets were higher; internet for nonacademic use, instance of absence, and stress were lower; and check-in time was earlier. The NLE1S had strong positive correlations with the comprehensive examination score and academic achievement during preclinical studies. By setting the NLE1S as a dependent variable in multivariate regression analyses, models of significant interactions were observed by setting behavioral factors, the comprehensive examination score, and academic achievement during a regular class as independent variables. Thus exhibiting good study habits and showing good academic performance throughout preclinical studies should be encouraged among students to achieve a good NLE1S. [ABSTRACT FROM AUTHOR]
- Published
- 2019
- Full Text
- View/download PDF
21. Impact of an early clinical exposure project conducted by senior clinical students compared between participating and nonparticipating students.
- Author
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Thanapat Vanichnatee, Chantacha Sitticharoon, Punyapat Maprapho, Issarawan Keadkraichaiwat, Nipith Charoenngam, and Rungnirand Praditsuwan
- Abstract
An early clinical exposure project conducted by clinical students aimed to promote direct clinical experience to preclinical students. The aim of this study was to determine the effects of the project on academic achievement and study attitudes and habits between participating and nonparticipating students before (test 1) and after the project (test 2) in the second preclinical year and at the end of the first semester of the first clinical year (test 3), with a subgroup analysis of the first (lowest) to third (highest) tertile of the score. Questionnaires were sent to the first clinical year students at test 3 and asked the information retrospectively at test 1 and test 2 in second year preclinical and currently at test 3, with 83.86% (265/316) being returned. Mean percentile of scores was higher at test 2 compared with test 1 in the first tertile group of participating students. Motivation to study medicine (motivation), realization of application of preclinical knowledge to clinical study (application), understanding of clinical environment (environment), and lesson review after class (review) were higher for participating than nonparticipating students at test 2 and/or test 3. Searching additional study information was higher at test 2 compared with test 1 only for participating students. This project could effectively promote application, motivation, environment, and review for participating vs. nonparticipating students at test 2 and/or test 3. Effortless, intimate, and effective communication between clinical and preclinical students and a direct experience in early clinical exposure might be key success factors. [ABSTRACT FROM AUTHOR]
- Published
- 2018
- Full Text
- View/download PDF
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