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Using the 9-fluorenylmethoxycarbonyl protecting group, 9-fluorenylmethoxycarbonyl-(S)-β-(N-imidazolyl)-α'-alanine protected non-protein amino acid, not described in the literature, were developed. Then 9-fluorenylmethoxycarbonyl-(S)-α-methylphenylalanine, 9-fluorenylmethoxycarbonyl-(S)-α-allylglycine, 9-fluorenylmethoxycarbonyl-(S)-α-propargylglycine were synthesized by the same method. It was shown, that the 9-fluorenylmethoxycarbonyl-(S)-β-(N-imidazolyl)-α-alanine (3) inhibited the growth of Gram-negative Salmonella typhimurium G-38 and 9-fluorenyl-methoxycarbonyl-(S)-α-methyl-phenylalanine (4) inhibited the growth Gram-positive Bacillus subtilis 17-89 bacteria. [ABSTRACT FROM AUTHOR] more...

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In recent years, fungal infections have emerged as a significant health concern across veterinary species, especially in livestock such as cattle, where fungal diseases can result in considerable economic losses, as well as in humans. In particular, Aspergillus species, notably Aspergillus flavus and Aspergillus versicolor , are opportunistic pathogens that pose a threat to both animals and humans. This study focuses on the synthesis and antifungal evaluation of novel 9-fluorenylmethoxycarbonyl (Fmoc)-protected 1,2,4-triazolyl-α-amino acids and their dipeptides, designed to combat fungal pathogens. More in detail, we evaluated their antifungal activity against various species, including Aspergillus versicolor (ATCC 12134) and Aspergillus flavus (ATCC 10567). The results indicated that dipeptide 7a exhibited promising antifungal activity against Aspergillus versicolor with an IC 50 value of 169.94 µM, demonstrating greater potency than fluconazole, a standard treatment for fungal infections, which showed an IC 50 of 254.01 µM. Notably, dipeptide 7a showed slightly enhanced antifungal efficacy compared to fluconazole also in Aspergillus flavus (IC 50 176.69 µM vs. 184.64 µM), suggesting that this dipeptide might be more potent even against this strain. Remarkably, 3a and 7a are also more potent than fluconazole against A. candidus 10711. On the other hand, the protected amino acid 3a demonstrated consistent inhibition across all tested Aspergillus strains, but with an IC 50 value of 267.86 µM for Aspergillus flavus , it was less potent than fluconazole (IC 50 184.64 µM), still showing some potential as a good antifungal molecule. Overall, our findings indicate that the synthesized 1,2,4-triazolyl derivatives 3a and 7a hold significant promise as potential antifungal agents in treating Aspergillus -induced diseases in cattle, as well as for broader applications in human health. Our mechanistic studies based on molecular docking revealed that compounds 3a and 7a bind to the same region of the sterol 14-α demethylase as fluconazole. Given the rising concerns about antifungal resistance, these amino acid derivatives, with their unique bioactive structures, could serve as a novel class of therapeutic agents. Further research into their in vivo efficacy and safety profiles is warranted to fully realize their potential as antifungal drugs in clinical and agricultural settings. more...