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22 results on '"Isola, Patrizia"'

3. Cocirculation of Hajj and non-Hajj strains among serogroup W meningococci in Italy, 2000 to 2016

Author

Fazio, Cecilia, primary, Neri, Arianna, additional, Vacca, Paola, additional, Ciammaruconi, Andrea, additional, Arghittu, Milena, additional, Barbui, Anna Maria, additional, Vocale, Caterina, additional, Bernaschi, Paola, additional, Isola, Patrizia, additional, Galanti, Irene Alessandra, additional, Mencacci, Antonella, additional, De Nittis, Rosella, additional, Chironna, Maria, additional, Giammanco, Anna, additional, Pagani, Elisabetta, additional, Bisbano, Alessandro, additional, and Stefanelli, Paola, additional

Published
2019
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5. Cytographic changes on BC-6800 Haematological Analyzer related to the presence ofCandida albicansin peripheral blood. A new tool to suspect candidemia?

Author

La Gioia, Antonio, primary, Devito, Alessandra, additional, Fiorini, Fabiana, additional, Bombara, Maria, additional, Isola, Patrizia, additional, Spinale, Barbara, additional, Francioni, Lucia, additional, Salamone, Domenico, additional, Marelli, Paola, additional, Buoro, Sabrina, additional, and Fiorini, Marcello, additional

Published
2016
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7. Cytographic changes on BC-6800 Haematological Analyzer related to the presence of Candida albicans in peripheral blood. A new tool to suspect candidemia?

Author

La Gioia, Antonio, Devito, Alessandra, Fiorini, Fabiana, Bombara, Maria, Isola, Patrizia, Spinale, Barbara, Francioni, Lucia, Salamone, Domenico, Marelli, Paola, Buoro, Sabrina, and Fiorini, Marcello

Subjects
CANDIDEMIA, BLOOD testing, CELL imaging, CANDIDA albicans, BLOOD sampling, DIAGNOSIS
Published
2017
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9. Tecnica e prospettive di utilizzo della citometria a flusso per la tipizzazione linfocitaria del liquido sinoviale di cane

Author

Marchetti, Veronica, Sbrana, Silvia, Modenato, Mario, Isola, Patrizia, Pistello, Mauro, Luchetti, Elena, Cardini, Giovanni, Marchetti, Veronica, Sbrana, Silvia, Modenato, Mario, Isola, Patrizia, Pistello, Mauro, Luchetti, Elena, and Cardini, Giovanni

Abstract

RIASSUNTO L’utilizzo della citometria a flusso, o fluorimetria, per l’immunofenotipizzazione linfocitaria è una metodica in uso in medicina umana soprattutto nel settore reumatologico, dove riveste un’importanza notevole nella comprensione dei meccanismi degenerativi cartilaginei in corso di artrite reumatoide, mentre è poco utilizzata nella più comune artrosi. In medicina veterinaria la tecnica non è a tutt’oggi utilizzata nella diagnostica collaterale dei problemi ortopedici e reumatologici. Sono disponibili solo due segnalazioni di lavori sperimentali in argomento. Sono state fatte numerose prove su campioni di liquido sinoviale di cane, prelevato sia durante la visita clinica che in sede intraoperatoria, in corso di patologie articolari diverse. Il liquido è stato sottoposto a conta cellulare con contaglobuli elettronico; per essere processato a tal fine e per l’analisi citofluorimetrica, è stato sottoposto a pretrattamento con jaluronidasi. La componente cellulare mononucleata è stata quindi separata e concentrata mediante centrifugazione in gradiente di densità su LymphoprepTM (Ficoll-Isopaque) e studiata con citofluorimetro. Contemporaneamente l’immunofenotipizzazione linfocitaria è stata condotta anche su un campione di sangue periferico. Prove successive finalizzate a rendere il campione un fluido acquoso hanno permesso di evidenziare l’assoluta necessità di pretrattare il campione con 2 gocce di jaluronidasi alla concentrazione di 150UI/ml per 0,25 cc di liquido sinoviale. La quantità di liquido prelevabile è in generale scarsa nei fatti degenerativi cronici, più abbondante nei fenomeni infiammatori acuti, e questo può rappresentare un limite nell’applicazione di questa metodica nella pratica clinica. Per ottenere una buona lettura è necessario avere infatti almeno 1cc di liquido articolare e/o almeno 1000 cellule/μl. La distribuzione delle sottopopolazioni linfocitarie CD3+ e CD21+, ed in particolare del rapporto fra i linfociti CD4+ e CD8+ nel liquido si

Published
2004

10. Cytographic changes on BC-6800 Haematological Analyzer related to the presence of Candida albicansin peripheral blood. A new tool to suspect candidemia?

Author

La Gioia, Antonio, Devito, Alessandra, Fiorini, Fabiana, Bombara, Maria, Isola, Patrizia, Spinale, Barbara, Francioni, Lucia, Salamone, Domenico, Marelli, Paola, Buoro, Sabrina, and Fiorini, Marcello

Abstract

BackgroundWe studied the quantitative and cytographic changes that the presence of Candida albicans(C. albicans) in peripheral blood (PB) samples causes on the Mindray BC-6800 Haematological Analyzer.MethodsA simulated in vitro candidemia was obtained by adding a different amount of C. albicansto discarded remnants of PB samples. Quantitative data and cytographic features were evaluated immediately as well as after 120 and 240 min of the yeast addition. A microscopic slides review was even performed at the same time.ResultsAfter yeasts addition, an increase of total leucocytes, neutrophils and basophils have been observed, but these increases are not certainly descriptive of C. albicanspresence.Instead, extracellular blastospores cause a false increase in nucleated red blood cells (nRBCs), which appear as a new population in the specific counting channel for erytroblasts (NRBC channel). Regardless of the numbers, C. albicansform a pseudo-erythroblastic cluster in the NRBC channel whose resulting shape is so different than the ‘normal’ nRBC that it demands a microscopic review. Even cytographic changes related with the neutrophilic phagocytic activity have been observed on leucocyte's differential count citogram (DIFF) of the BC-6800.ConclusionsOur observations suggest that the results of the BC-6800, which are due to C. albicans' presence, might be useful to speculate earlier diagnosis of sepsis.

Published
2017
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12. High level of messenger RNA forBRMS1 in primary breast carcinomas is associated with poor prognosis

Author

Lombardi, Grazia, primary, Di Cristofano, Claudio, additional, Capodanno, Alessandra, additional, Iorio, Maria Carla, additional, Aretini, Paolo, additional, Isola, Patrizia, additional, Tancredi, Mariella, additional, Collecchi, Paola, additional, Naccarato, Antonio Giuseppe, additional, Porta, Romana Prosperi, additional, Bevilacqua, Generoso, additional, and Caligo, Maria Adelaide, additional

Published
2007
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13. AIDS Vaccination Studies with an Ex Vivo Feline Immunodeficiency Virus Model: Analysis of the Accessory ORF-A Protein and DNA as Protective Immunogens

Author

Pistello, Mauro, primary, Bonci, Francesca, additional, Flynn, J. Norman, additional, Mazzetti, Paola, additional, Isola, Patrizia, additional, Zabogli, Elisa, additional, Camerini, Valentina, additional, Matteucci, Donatella, additional, Freer, Giulia, additional, Pelosi, Paolo, additional, and Bendinelli, Mauro, additional

Published
2006
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16. AIDS Vaccination Studies Using an Ex Vivo Feline Immunodeficiency Virus Model: Protection from an Intraclade Challenge Administered Systemically or Mucosally by an Attenuated Vaccine

Author

Pistello, Mauro, primary, Matteucci, Donatella, additional, Bonci, Francesca, additional, Isola, Patrizia, additional, Mazzetti, Paola, additional, Zaccaro, Lucia, additional, Merico, Antonio, additional, Del Mauro, Daniela, additional, Flynn, Norman, additional, and Bendinelli, Mauro, additional

Published
2003
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17. AIDS Vaccination Studies Using an Ex Vivo Feline Immunodeficiency Virus Model: Failure To Protect and Possible Enhancement of Challenge Infection by Four Cell-Based Vaccines Prepared with Autologous Lymphoblasts

Author

Giannecchini, Simone, primary, Isola, Patrizia, additional, Sichi, Olimpia, additional, Matteucci, Donatella, additional, Pistello, Mauro, additional, Zaccaro, Lucia, additional, Del Mauro, Daniela, additional, and Bendinelli, Mauro, additional

Published
2002
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18. Dynamics of Persistent TT Virus Infection, as Determined in Patients Treated with Alpha Interferon for Concomitant Hepatitis C Virus Infection

Author

Maggi, Fabrizio, primary, Pistello, Mauro, additional, Vatteroni, Marialinda, additional, Presciuttini, Silvano, additional, Marchi, Santino, additional, Isola, Patrizia, additional, Fornai, Claudia, additional, Fagnani, Sabina, additional, Andreoli, Elisabetta, additional, Antonelli, Guido, additional, and Bendinelli, Mauro, additional

Published
2001
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19. Decay of HIV Type 1 DNA and Development of Drug-Resistant Mutants in Patients with Primary HIV Type 1 Infection Receiving Highly Active Antiretroviral Therapy

Author

Riva, Elisabetta, primary, Pistello, Mauro, additional, Narciso, Pasquale, additional, D'Offizi, Gianpiero, additional, Isola, Patrizia, additional, Galati, Vincenzo, additional, Turriziani, Ombretta, additional, Tozzi, Valerio, additional, Vincenzi, Laura, additional, Dianzani, Ferdinando, additional, and Antonelli, Guido, additional

Published
2001
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21. Immunogenicity of an Anti-Clade B Feline Immunodeficiency Fixed-Cell Virus Vaccine in Field Cats

Author

Matteucci, Donatella, primary, Poli, Alessandro, additional, Mazzetti, Paola, additional, Sozzi, Sabrina, additional, Bonci, Francesca, additional, Isola, Patrizia, additional, Zaccaro, Lucia, additional, Giannecchini, Simone, additional, Calandrella, Michela, additional, Pistello, Mauro, additional, Specter, Steven, additional, and Bendinelli, Mauro, additional

Published
2000
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22. Adoptive immunotherapy of feline immunodeficiency virus with autologous ex vivo-stimulated lymphoid cells modulates virus and T-cell subsets in blood.

Author

Flynn JN, Pistello M, Isola P, Zaccaro L, Del Santo B, Ricci E, Matteucci D, and Bendinelli M

Subjects
Animals, CD4-CD8 Ratio, CD4-Positive T-Lymphocytes immunology, CD8-Positive T-Lymphocytes immunology, Cat Diseases therapy, Cat Diseases virology, Cats, Cells, Cultured, DNA, Viral analysis, Female, Fibroblasts metabolism, Fibroblasts virology, Gene Products, env metabolism, Gene Products, gag metabolism, Immunodeficiency Virus, Feline genetics, Immunodeficiency Virus, Feline pathogenicity, Interferon-gamma biosynthesis, Lentivirus Infections therapy, Lentivirus Infections veterinary, Leukocytes, Mononuclear cytology, Lymph Nodes cytology, Membrane Glycoproteins metabolism, T-Lymphocytes metabolism, Vaccinia virus genetics, Vaccinia virus immunology, Cat Diseases immunology, Immunodeficiency Virus, Feline immunology, Immunotherapy, Adoptive, Lentivirus Infections immunology, T-Lymphocytes immunology
Abstract

The potential of immunotherapy with autologous virus-specific T cells to affect the course of feline immunodeficiency virus (FIV) infection was explored in a group of specific-pathogen-free cats infected with FIV a minimum of 10 months earlier. Popliteal lymph node cells were stimulated by cocultivation with UV-inactivated autologous fibroblasts infected with recombinant vaccinia viruses expressing either FIV gag or env gene products, followed by expansion in interleukin-2. One or two infusions of both Gag- and Env-stimulated cells resulted in a slow increase in FIV-specific gamma interferon-secreting T cells in the circulation of cats. In the same animals, viral set points fluctuated widely during the first 2 to 3 weeks after adoptive transfer and then returned to pretreatment levels. The preexisting viral quasispecies was also found to be modulated, whereas no novel viral variants were detected. Circulating CD4(+) counts underwent a dramatic decline early after treatment. CD4/CD8 ratios remained instead essentially unchanged and eventually improved in some animals. In contrast, a single infusion of Gag-stimulated cells alone produced no apparent modulations of infection.

Published
2005
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