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The research aimed to analyze the effect of young green coconut water diluent with egg yolk in Boer goat liquid semen quality at cold storage. This study used an experimental laboratory with 4 treatments and 10 replications. The treatments tested were: P0 = CEP-3 + 10% egg yolk + 0.4% egg white, P1 = 90% coconut water + 10% egg yolk, P2 = 85% coconut water + 15% egg yolk, and P3 = 80% coconut water + 20% egg yolk. The variables analysis was individual motility, viability, abnormality, intact plasma membrane, and intact acrosome hood. The data were analyzed using Analysis of Variance (ANOVA), and the difference between treatments continued with Duncan Multiple Range Test. The result showed significant differences in individual motility, viability, intact plasma membrane, and intact acrosome hood (P0.05). In conclusion, the combination of coconut water + 20% egg yolk could be recommended for the diluent of Boer goat liquid semen in cold storage for up to 3 days.

The utilization of sexing technology is an effort made to improve the efficiency of livestock farming, which was created to predict the sex of the calves born so that it can be adjusted to the objectives of the farm. This study aims to analyze the quality and proportion of sexed spermatozoa using the percoll density centrifugation method at different gradients with AndroMed® diluent. The research material used was fresh semen from Belgian Blue Bull aged six years with a body weight of 600 kg and fresh semen quality motility ≥70%. This research is a laboratory experiment with two treatments and ten replicates. The treatments in the study were T0: Ten Gradient density percoll + AndroMed® and T1: Five Gradient density percoll + AndroMed®. The research analysis used a dependent t-test. The statistical analysis showed a significant difference (P0.05) in the treatment of 10 gradients with five gradients. The average results on individual motility, abnormality, concentration, and total motile spermatozoa showed treatment of 10 gradients better than treatment of 5 gradients in each layer. At the same time, the variable viability showed that the gradient treatment is better than the five-gradient treatment. Proportion of spermatozoa in the Upper Layer T0 X (23.4%), Y (76.8%) and T1 X (16.9%), Y (83.1%). The proportion of Spermatozoa in the Bottom Layer was T0 X (84.1%), Y (15.9%), and T1 X (83%), Y (17%), respectively. In conclusion, sexing spermatozoa with the Percoll 10 and 5 Gradient Density Centrifugation method can separate X and Y spermatozoa.

This study investigated the effects of utilizing a mixture of slaughterhouse waste and rice bran as a growing medium on the morphometric traits of Black Soldier Fly (BSF) larvae. The research followed a completely randomized design with five treatment groups, namely T0: 100% slaughterhouse waste (control), T1: 75% slaughterhouse waste + 25% rice bran, T2: 50% slaughterhouse waste + 50% rice bran, T3: 25% slaughterhouse waste + 75% rice bran, and T4: 100% rice bran. The morphometric traits examined in this study included the larvae body length at 19 days (LBL19), larvae body width at 19 days (LBW19), larvae body length at 29 days (LBL29), and larvae body width at 29 days (LBW29). The results demonstrated that using slaughterhouse waste as the sole medium (T0) could support the growth of BSF larvae, but the morphometric traits of larvae in T0 were relatively modest. Interestingly, when RB was used to replace SW partially, it had a dose-dependent effect. T1 significantly improved (P < 0.05) LBL19, LBL29, and LBW29. Conversely, the morphometric traits of BSF larvae in T2 and T3 did not exhibit significant changes (P > 0.05) compared to the control. Moreover, the use of T4 significantly enhanced (P < 0.05) all morphometric traits of BSF larvae (LBL19, LBW19, LBL29, and LBW29). In conclusion, this study reveals the significant potential of BSF in managing slaughterhouse waste. Furthermore, employing a growing medium consisting of 75% slaughterhouse waste and 25% rice bran emerges as a practical approach to strike a harmonious balance between effective waste management and fostering the development of BSF larvae.

This study aimed to evaluate the development of Black Soldier Fly (BSF) larvae when reared on different feeding media, specifically using varying ratios of fermented laying hen manure and starter feed. The study employed a completely randomized design with five treatments and four replications. The treatments were as follows: T0: 100% fermented laying hen manure, T1: 75% fermented laying hen manure + 25% laying hen starter feed, T2: 50% fermented laying hen manure + 50% laying hen starter feed, T3: 25% fermented laying hen manure + 75% laying hen starter feed, and T4: 100% laying hen starter feed. Parameters measured in the study included larvae length and larvae width at 10 and 17 days. Data were analyzed using one-way analysis of variance followed by the Duncan post-hoc test. The study demonstrated that BSF larvae were successfully reared on growing media comprising 100% fermented laying hen manure (T0). However, the development of larvae in T0 remained relatively low. Notably, the use of laying hen starter feed particularly at 75% (T3) and 100% (T4) significantly improved (P

This study aimed to determine the success of artificial insemination (AI) using unsexed and sexed frozen semen with albumin sedimentation. This research was conducted from August to December 2022 at a farmer community in Pandesari Village, Pujon District, Malang Regency. The material used in this study was 78 heads of Friesian Holstein cattle, with the criteria of having a minimum body condition score of 2.5 (scale of 1–5), having normal reproductive organs, and showing signs of estrus. 38 cows were used as acceptors in artificial insemination (AI) with unsexed frozen semen, and 38 cows in AI with albumin sedimentation frozen semen. The variables used are non-return rate 1, non-return rate 2, and conception rate. The data obtained were then analyzed descriptively. The results showed that the percentage values of NRR1, NRR2, and CR in AI using frozen semen unsexed were 76.3%, 63.1%, and 47.3%, respectively, while the percentage values of NRR1, NRR2, and CR in AI using frozen semen sexed were 94.7%, 86.15%, and 63.15%. Based on the study's results, insemination success using albumin sedimentation frozen semen sexed showed better results than AI using unsexed semen.

The impact of supplementing glucose, glycine, or a combination of both in Ringer’s lactate egg yolk base extender to preserve the quality of semen from local Indonesian chickens has not been previously investigated. This study aimed to examine the potential of glucose and glycine on chicken semen stored at 5°C for 120 hours. In this study, five local roosters were used. The parameters under observation included semen volume, odor, pH levels, consistency, color, mass movement, concentration, motility, viability, abnormality, plasma membrane integrity, chromatin degeneration, and acrosomal cap integrity. This study was conducted using a completely randomized design (CRD) with four treatments groups and 10 replication, i.e. T1 (control without supplementation), T2 (50 mM glucose), T3 (60 mM glycine), and T4 (a combination of 50 mM glucose and 60 mM glycine), respectively. In result, semen volume was 0.54 ± 0.17 mL/ejaculate, a milky white color, distinctive odor, thick consistency, good mass movement (++/+++), pH of 7.37 ± 0.23, motility of 91.50 ± 2.42%, plasma membrane integrity of 96.85 ± 0.96%, abnormality at 2.88 ± 0.77%, the concentration of 3.04 ± 0.3 billion/mL, and viability of 96.47 ± 1.71%. Following storage at 5°C for 120 hours, the motility, viability, abnormality, and acrosomal cap integrity of local chicken spermatozoa significantly different (p < 0.05) between T3 and T4 compared to T1 and T2 groups. Moreover, the integrity of the plasma membrane and chromatin degeneration in treatment T3 significantly different (p < 0.05) from T1, T2, and T4 groups. In conclusion, local chickens exhibited fair quality fresh semen both in macroscopic and microscopic evaluations. Furthermore, the combination of 60 mM glycine and 50 mM glucose into local chicken semen stored at 5°C for 120 hours effectively preserved motility and viability, minimized abnormality, maintained plasma membrane integrity, minimized chromatin degeneration, and retained acrosomal integrity. [ABSTRACT FROM AUTHOR]

This study aimed at finding the effect of ladder drill training upon: (1) run speed, (2) agility, and (2) power of leg muscle. This study is an experimental research. This study utilized one group pre test-post test design. There were total people as the subject of this research. Data collection technique used 30-meter sprint test to measure run speed, Illinois agility test to measure agility, and vertical jump test to measure power of leg muscle. Data analysis technique which was used for normality test, homogeneity test/F-test, and T-test with significant level 5% by using SPSS 16.0.0. Based on the finding, there was effect of ladder drill training upon run speed with sig value=0.007, agility and power of leg muscle with sig value=0.000. Based on the data analysis, it could be concluded that there was significant effect of ladder drill training upon run speed, agility and power of leg muscle.

This research was to compare semen quality and quantity of simmental cattle on the variation of weight. The research used six heads of cattle. The method of this research was survey method and analyze data using variety analyzing method, thus would be continued using duncan test if there are present significan differences. Simmental cattle at medium weight (840 and 846 kg) had more quality than other two groups of weight, which showed from highest scale of colour, and consistency. Simmental cattle at low weight (764 and 797 kg) showed a highest scale of pH and mass motility rather than other two groups of weight. The research of quantity of Simmental cattle at medium weight (840 and 846 kg) group showed highest scale of volume average at 8,5 ± 0,92 ml, individual motility average at 60,6 ± 7,95% and amount of motil spermatozoa average at 6.082,6 ± 1.204,06 (106) rather than other groups. Highest scale concentration had showed on low weight (746 and 797 kg) Simmental cattle group average at 1.355,7 ± 153,60 (106/ml). The high weight (942 and 952 kg) group of Simmental cattle showed highest amount of spermatozoa average at 10.826,9 ± 1.777,16 (106). Simmental cattle at medium weight (840-846 kg) had better semen quality and quantity than others. Keywords : Simmental cattle, cattle weight, quality and quantity of semen