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A study was undertaken to determine typical concentrations of airborne bacteria and fungi (microflora) in Teaching Hospital environment in Benin City in the tropical rainforest environment of Nigeria. Aerial sampling wasconducted at various hospital wards each day. The air samples were collected thrice daily, that is, in the morning, between 8am and 10am, in the afternoon, between 12noon and 2pm and in the evening between 4pm and 6pm. Concentrations of airborne microflora exceeded available local guidelines for indoor quality in the accident and emergency ward, female ward, male ward, pediatric ward and the maternity ward, but not in the restricted wards like the theatre, intensive care unit and bacteriological laboratory. Results showed that the occupant density was the keyfactor influencing the levels of airborne microflora, while humidity was also observed as a factor, depending on the particular location with the hospital. The concentration of airborne bacteria and fungi in the nine different hospital units varied from wards to wards. The bacterial population ranges from 3.0cf/m3 to 76.0cf/m3, with the highest bacterial population recorded in the accident and emergency ward. The fungal population ranges from 6.0cf/m3 to 44.7cf/m3, while the highest fungal population was recorded in the accident and emergency ward. The microflora characterized and identified, were representative ofthe normal microflora of the human body (skin, gastrointestinal tracts, respiratory tract) and the opportunistic pathogens. The microbial isolates included six bacterial genera, among which are, Staphylococcus aureus,Staphylococcus epidermis, Escherichia coli, Bacillus sp. and Proteus mirabilis, the fugal isolates included, Aspergillus sp, Penicillium sp., Mucor sp., Candida sp and Verticillium sp. The variations in hospital units in concentrations of total airborne microorganisms (bacteria and fungi) in the air of hospital environments were statistically significant (p