102 results on '"Ishizuka R"'
Search Results
2. Greater milk intake is associated with lower bone turnover, higher bone density, and higher bone microarchitecture index in a population of elderly Japanese men with relatively low dietary calcium intake: Fujiwara-kyo Osteoporosis Risk in Men (FORMEN) Study
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Sato, Y., Iki, M., Fujita, Y., Tamaki, J., Kouda, K., Yura, A., Moon, J.-S., Winzenrieth, R., Iwaki, H., Ishizuka, R., Amano, N., Tomioka, K., Okamoto, N., and Kurumatani, N.
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- 2015
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Catalog
3. Conformational equilibria in liquids consisting of small chain molecules
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Ishizuka, R. and Hirata, F.
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- 2006
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4. Root surface conditioning with bone morphogenetic protein-2 facilitates cementum-like tissue deposition in beagle dogs
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Miyaji, H., Sugaya, T., Ibe, K., Ishizuka, R., Tokunaga, K., and Kawanami, M.
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- 2010
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5. Application of efficient algorithm for solving six-dimensional molecular Ornstein-Zernike equation.
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Ishizuka, R. and Yoshida, N.
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ALGORITHMS , *STATISTICAL mechanics , *ITERATIVE methods (Mathematics) , *INTEGRAL equations , *NUMERICAL integration , *STATISTICAL correlation , *PERMITTIVITY - Abstract
In this article, we propose an efficient algorithm for solving six-dimensional molecular Ornstein-Zernike (MOZ) equation. In this algorithm, the modified direct inversion in iterative subspace, which is known as the fast convergent method for solving the integral equation theory of liquids, is adopted. This method is found to be effective for the convergence of the MOZ equation with a simple initial guess. For the accurate averaging of the correlation functions over the molecular orientations, we use the Lebedev-Laikov quadrature. The appropriate number of grid points for the quadrature is decided by the analysis of the dielectric constant. We also analyze the excess chemical potential of aqueous ions and compare the results of the MOZ with those of the reference interaction site model. [ABSTRACT FROM AUTHOR] more...
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- 2012
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6. An integral equation theory for inhomogeneous molecular fluids: The reference interaction site model approach.
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Ishizuka, R., Chong, S.-H., and Hirata, F.
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INTEGRAL equations , *LIQUIDS , *HYDRATION , *CHEMISTRY , *PHYSICS - Abstract
An integral equation theory which is applicable to inhomogeneous molecular liquids is proposed. The “inhomogeneous reference interaction site model (RISM)” equation derived here is a natural extension of the RISM equation to inhomogeneous systems. This theory makes it possible to calculate the pair correlation function between two molecules which are located at different density regions. We also propose approximations concerning the closure relation and the intramolecular susceptibility of inhomogeneous molecular liquids. As a preliminary application of the theory, the hydration structure around an ion is investigated. Lithium, sodium, and potassium cations are chosen as the solute. Using the Percus trick, the local density of solvent around an ion is expressed in terms of the solute-solvent pair correlation function calculated from the RISM theory. We then analyze the hydration structure around an ion through the triplet correlation function which is defined with the inhomogeneous pair correlation function and the local density of the solvent. The results of the triplet correlation functions for cations indicate that the thermal fluctuation of the hydration shell is closely related to the size of the solute ion. The triplet correlation function from the present theory is also compared with that from the Kirkwood superposition approximation, which substitutes the inhomogeneous pair correlation by the homogeneous one. For the lithium ion, the behavior of the triplet correlation functions from the present theory shows marked differences from the one calculated within the Kirkwood approximation. [ABSTRACT FROM AUTHOR] more...
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- 2008
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7. 115 Need for Repeat Measurement of Free Testosterone in the Diagnosis of Testosterone Deficiency
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Suetomi, T., primary, Kurobe, M., additional, Ikeda, A., additional, Ishizuka, R., additional, Kimura, T., additional, Waku, N., additional, Kojima, T., additional, Joraku, A., additional, Miyazaki, J., additional, Kawai, K., additional, and Nishiyama, H., additional more...
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- 2017
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8. Erratum: “Application of efficient algorithm for solving six-dimensional molecular Ornstein-Zernike equation” [J. Chem. Phys. 136, 114106 (2012)]
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Ishizuka, R., primary and Yoshida, N., additional
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- 2014
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9. Leucine Affects Growth and Hepatic Growth-Related Factor Gene Expression in Japanese AmberjackSeriola quinqueradiata
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Kawanago, M., primary, Takemura, S., additional, Ishizuka, R., additional, Kousaka, T., additional, and Shioya, I., additional
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- 2014
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10. Dietary branched-chain amino acid supplementation affects growth and hepatic insulin-like growth factor gene expression in yellowtail,Seriola quinqueradiata
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Kawanago, M., primary, Takemura, S., additional, Ishizuka, R., additional, and Shioya, I., additional
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- 2014
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11. Plasma Components and Hepatic Insulin-like Growth Factor Expression Indicate Nutritional Status in YellowtailSeriola quinqueradiata
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Kawanago, M., primary, Takemura, S., additional, Ishizuka, R., additional, and Shioya, I., additional
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- 2014
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12. Ab initio theoretical study of temperature and density dependence of molecular and thermodynamic properties of water in the entire fluid region: Autoionization processes
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Yoshida, N, Ishizuka, R, Sato, H, Hirata, F, Yoshida, N, Ishizuka, R, Sato, H, and Hirata, F
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- 2006
13. Ab initio theoretical study of temperature and density dependence of molecular and thermodynamic properties of water in the entire fluid region: Autoionization processes
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70290905, Yoshida, N, Ishizuka, R, Sato, H, Hirata, F, 70290905, Yoshida, N, Ishizuka, R, Sato, H, and Hirata, F
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- 2006
14. Dynamics of the solvent around a solute: Generalized Langevin theory
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Ishizuka, R., primary and Hirata, F., additional
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- 2010
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15. Dietary branched-chain amino acid supplementation affects growth and hepatic insulin-like growth factor gene expression in yellowtail, Seriola quinqueradiata.
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Kawanago, M., Takemura, S., Ishizuka, R., and Shioya, I.
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SOMATOMEDIN ,YELLOWTAIL ,GENE expression in fishes ,JAPANESE amberjack ,AMINO acids in nutrition ,FISH feeds - Abstract
We investigated the effect of dietary supplementation with a branched-chain amino acid ( BCAA) mixture (valine, leucine and isoleucine) on juvenile yellowtail ( Seriola quinqueradiata) growth and hepatic insulin-like growth factor ( IGF) gene expression. Total dietary BCAA content was 8.1, 8.5, 9.0 and 9.8 g kg
−1 for the control, 0.4%, 1.0% and 2.0% BCAA experimental groups, respectively. Body weight was measured on day 77, after which fish were fasted for 3 days and refed; the livers were then removed 3 or 9 h later for analysis of hepatic gene expression. Body weight significantly increased in the BCAA-supplemented groups. Hepatic expression of IGF1 and IGF2 significantly increased in the 2% BCAA group at 3 and 9 h after refeeding. In the 1% BCAA group, hepatic IGF1 expression tended to be higher at 9 h than in the control group after refeeding. Also, hepatic IGF2 significantly increased at only 9 h after refeeding in the 1% BCAA group. In conclusion, dietary supplementation with crystalline BCAAs increased growth and hepatic expression of IGF1 and IGF2. These results suggest that dietary crystalline BCAA supplementation would be a valuable addition to yellowtail aquaculture practices. [ABSTRACT FROM AUTHOR] more...- Published
- 2015
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16. Leucine Affects Growth and Hepatic Growth-Related Factor Gene Expression in Japanese Amberjack Seriola quinqueradiata.
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Kawanago, M., Takemura, S., Ishizuka, R., Kousaka, T., and Shioya, I.
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LEUCINE ,GENE expression in fishes ,YELLOWTAIL ,BRANCHED chain amino acids ,ESSENTIAL amino acids - Abstract
We examined the effect of dietary supplementation of leucine on body weight and hepatic insulin-like growth factor (IGF) and IGF-binding protein (IGFBP) gene expression in juvenile Japanese AmberjackSeriola quinqueradiata. Crystalline leucine was dissolved in deionized water and added to the basal diet. Deionized water only was added to the basal diet as a control. The dietary concentration of free leucine was 0.03, 0.85, and 2.43 g/100 g diet for the control, 1% leucine-supplemented, and 3% leucine-supplemented diets, respectively. A growth trial was conducted for 77 d, after which the body weight was measured and the fish were then fasted for 3 d and refed. At 3, 9, and 24 h after refeeding, the liver and muscle were dissected for gene expression analysis. The mean body weight significantly increased in the 1% leucine-supplemented group during the growth trial. The expression of hepaticIGBFP-1significantly increased at 3 and 9 h after refeeding in the leucine-supplemented groups. The expression of hepaticIGF1significantly decreased at 9 h and that ofIGF2significantly decreased at 9 and 24 h after refeeding in the 3% leucine-supplemented group. The expression of hepaticIGFBP-2,IGFBP-3, andIGFBP-5were similar among the groups and were only affected by feeding time. In conclusion, we demonstrated for the first time that leucine supplementation increases body weight and influences the hepatic growth factor system in Japanese Amberjack. Received March 19, 2014; accepted June 9, 2014 [ABSTRACT FROM PUBLISHER] more...
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- 2014
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17. Plasma Components and Hepatic Insulin-like Growth Factor Expression Indicate Nutritional Status in Yellowtail Seriola quinqueradiata.
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Kawanago, M., Takemura, S., Ishizuka, R., and Shioya, I.
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JAPANESE amberjack ,INSULIN-like growth factor receptors ,BILIRUBIN derivatives ,STIMULUS satiation ,GENE expression ,BEHAVIOR - Abstract
We examined the effect of feeding ration and refeeding time on plasma biochemical components and hepatic insulin-like growth factor gene (IGF) mRNA expression to evaluate nutritional indicators in juvenile YellowtailSeriola quinqueradiata(also known as Buri). Fish were fed a diet for 10 d with the following feeding rations: 80, 40, 20, and 10% satiation. Satiated feeding was 4.65% of body weight. At day 10, the plasma concentrations of albumin, calcium, inorganic phosphate, and total protein, activities of alkaline phosphatase and amylase, and hepaticIGF1mRNA andIGF2mRNA expression were significantly correlated with feeding ration. In the analysis of refeeding time, fish were refed after a 24-h fasting period and plasma biochemical components and hepatic IGFs mRNA were analyzed at 0, 3, 6, and 9 h after refeeding. Plasma concentrations of total bilirubin, inorganic phosphate, creatinine, glucose, and potassium, and activities of amylase were significantly correlated with refeeding time. The correlation between refeeding time and gene expression was significant forIGF2mRNA (r= 0.70,P< 0.05) but not forIGF1mRNA (r= 0.67,P= 0.07). Comparing the results of the two experiments, the plasma concentrations of total protein, albumin, and calcium reflected only the feeding ration. The plasma concentration of inorganic phosphate, activities of alkaline phosphatase and amylase, and hepaticIGF1mRNA andIGF2mRNA reflected both the feeding ration and refeeding time. These observations suggest that these components reflected the nutritional status of Yellowtail, but sampling time should be carefully examined because of the fluctuating feature of these components. Received September 29, 2013; accepted December 14, 2013 [ABSTRACT FROM AUTHOR] more...
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- 2014
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18. Biochemical analysis of HLA‐DR antigens using one‐dimensional isoelectric focusing gel electrophoresis
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Ishizuka, R., primary, Kaidoh, T., additional, Matsuo, T., additional, Koide, Y., additional, and Yoshida, T. O., additional
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- 1992
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19. Application of glucuronic acid to ovarian disorder of cow
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MOTOYOSHI, S., primary, KANO, Y., additional, MIURA, T., additional, and ISHIZUKA, R., additional
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- 1964
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20. Fully compatible integration of high density embedded DRAM with 65nm CMOS technology (CMOS5)
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Matsubara, Y., primary, Habu, M., additional, Matsuda, S., additional, Honda, K., additional, Morifuji, E., additional, Yoshida, T., additional, Kokubun, K., additional, Yasumoto, K., additional, Sakurai, T., additional, Suzuki, T., additional, Yoshikawa, J., additional, Takahashi, E., additional, Hiyama, K., additional, Kanda, M., additional, Ishizuka, R., additional, Moriuchi, M., additional, Koga, H., additional, Fukuzaki, Y., additional, Sogo, Y., additional, Takahashi, H., additional, Nagashima, N., additional, Okamoto, Y., additional, Yamada, S., additional, and Noguchi, T., additional more...
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21. A combination of gabor wavelets and snakes for a mobile-phone-oriented hair-style simulation system
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Sandoval, H., primary, Ishizuka, R., additional, Chigusa, Y., additional, and Hattori, T., additional
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22. Integration of interconnect process highly manufacturable for 65nm CMOS platform technology (CMOS5).
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Honda, K., Kanda, M., Ishizuka, R., Moriuchi, Y., Matsubara, Y., Habu, M., Yoshida, T., Matsuda, S., Kittaka, H., Miyajima, H., Hachiya, T., Kajita, A., Usui, T., Nagashima, N., Kanamura, R., Okamoto, Y., Yamada, S., and Noguchi, T. more...
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- 2004
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23. GASLESS LAPAROSCOPIC CHOLECYSTECTOMY USING A SIMPLE ABDOMINAL WALL LIFTING DEVICE.
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Kurauchi, N., Ito, Y., Sato, T., Onodera, I., and Ishizuka, R.
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- 1996
24. Martini 3D-OZ: A Theoretical Investigation of Solvation Shell Structures and Solvation Free Energies of Martini Coarse-Grained Proteins.
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Ishizuka R
- Abstract
We investigate the properties of aqueous solutions using integral equation theories and molecular dynamics (MD) simulations within the framework of the MARTINI coarse-grained force field. The integral equation theory used in the present work is based on the Ornstein-Zernike equation coupled with the hypernetted chain (HNC) and Kovalenko-Hirata (KH) closures. Overall, the solvation shell structures and solvation thermodynamics in the HNC approximation are shown to be in better agreement with those from the MD simulation than the KH results. Especially, through the analysis of spatial distribution functions of water around a protein, we have demonstrated that the HNC approximation can provide the highly anisotropic structure of the solvation shell of the protein. On the other hand, the KH approximation works well for simple particle solutes, but the results for highly hydrated proteins deviate quite significantly from the MD results. We further explore in detail the reason underlying the deviation caused by the KH approximation. Lastly, a potential application of the integral equation theory with the MARTINI model is outlined. more...
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- 2024
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25. Effect of digital messages from health professionals on COVID-19-related outcomes: A systematic review of randomized controlled trials.
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Kuwahara K, Sakamoto M, Ishizuka R, Kato M, Akiyama M, Ishikawa H, and Kiyohara K
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- Humans, Randomized Controlled Trials as Topic, Health Personnel, Databases, Factual, COVID-19 prevention & control, Physicians
- Abstract
Effectiveness of health professionals' messages via digital tools on COVID-19-related outcomes remains unknown. In this systematic review, we examined this issue by searching randomized controlled trials in six databases until August, 2022 (PROSPERO, CRD 42022350788). Risk of bias was assessed using revised Cochrane risk-of-bias tool. Primary outcomes were COVID-19 infections, preventive behaviors, and factors that support behavioral change. Seven trials with 16 reports (some reported two or more outcomes) were included. One trial showed that physician videos distributed by Facebook ads significantly reduced COVID-19 infections. Five of 6 reports showed that interventions can promote non-pharmaceutical preventive behaviors, vaccination, or information-seeking behaviors and half of the results were significant. Interventions improved the factors that support behavioral change. 14 reports had some concerns of bias risk and 2 had high risk of bias. Our findings suggest that digital messages by health professionals may help reduce COVID-19 infections through promoting behavioral change., Competing Interests: Declaration of Competing Interest There is no conflict of interest relating to this manuscript. Dr Kuwahara received lecture fee from Pfizer Inc. outside of this work. No other financial disclosures were reported. This study was funded by the MHLW Research Program on Emerging and Reemerging Infectious Diseases (Grant Number JPMH21HA2011) from the Ministry of Health, Labour and Welfare of Japan. The funder had no role in the design and conduct of the study; collection, management, analysis, and interpretation of the data; preparation, review, or approval of the manuscript; and decision to submit the manuscript for publication. The authors are thankful to Drs Akiko Tamakoshi, Wakaba Fukushima, and other members of Japan Epidemiological Association for their supporting to obtain the funding., (Copyright © 2023 The Author(s). Published by Elsevier Ltd.. All rights reserved.) more...
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- 2023
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26. Breakfast Skipping and Declines in Cognitive Score Among Community-Dwelling Older Adults: A Longitudinal Study of the HEIJO-KYO Cohort.
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Ishizuka R, Otaki N, Tai Y, Yamagami Y, Tanaka K, Morikawa M, Iki M, Kurumatani N, Saeki K, and Obayashi K
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- Humans, Longitudinal Studies, Independent Living, Prospective Studies, Cognition, Feeding Behavior psychology, Breakfast psychology
- Abstract
Previous studies outlined the correlation of adverse effects of breakfast skipping with cognitive function. However, the majority of these studies have focused on the short-term effects; to date, the long-term effect of breakfast skipping on cognitive function among older adults remains unclear. In this prospective cohort study of 712 older adults (mean age, 70.8 years), breakfast skipping was defined as skipping breakfast one or more times per week, and declines in cognitive score was defined as decreases in Mini-Mental State Examination (MMSE) score of two or more in the observed period. During follow-up (median, 31 months), 135 of 712 participants developed declines in cognitive score. Poisson regression models revealed that the incidence rate for declines in cognitive score was significantly higher in breakfast skipper (n = 29) than breakfast eaters (n = 683) [incidence rate ratio (IRR), 2.10; 95% CI, 1.28-3.44]. Additional propensity score adjustments related to breakfast skipping from baseline parameters (age, gender, smoking and drinking status, BMI, household income, educated level, depressive symptoms, hypertension, diabetes, sleep medication, physical activity, caloric intake, and baseline cognition) produced consistent results (IRR, 2.21; 95% CI, 1.33-3.68). Sensitivity analysis, when the cut-off value of decreases in MMSE score was changed to three points, suggested a significant and stronger association (IRR, 3.03; 95% CI, 1.72-5.35). Regarding daily intakes of food groups, breakfast skippers consumed a significantly lower amount of vegetables, fruits, and fish than breakfast eaters. In conclusion, our findings suggest that breakfast skipping is longitudinally associated with declines in cognitive score among older adults. more...
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- 2023
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27. Health information and COVID-19 vaccination: Beliefs and attitudes among Japanese university students.
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Sakamoto M, Ishizuka R, Ozawa C, and Fukuda Y
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- Young Adult, Adolescent, Humans, Female, Universities, COVID-19 Vaccines, Japan epidemiology, Cross-Sectional Studies, Health Knowledge, Attitudes, Practice, Vaccination, Students, Surveys and Questionnaires, COVID-19 epidemiology, COVID-19 prevention & control, Vaccines
- Abstract
Coronavirus disease 2019 (COVID-19) vaccination of adolescent and young adults is important for infection control. Factors influencing vaccination behavior in this age group include the source of information about the vaccine and social norms. However, there are few studies on factors influencing vaccination behavior among Japanese university students. This study aimed to assess beliefs, attitudes, and sources of information about the COVID-19 vaccine among university students in Japan. A cross-sectional online questionnaire survey was conducted among students in Teikyo University, Japan in September, 2021. The survey was designed to collect demographic information, vaccination status, attitudes, beliefs, and anxiety about the vaccine, sources of information, and whether or not the participant watched an educational movie. The factors associated with beliefs and attitudes were analyzed using logistic and linear regression. A total of 4,062 valid questionnaires were retrieved. The positive vaccine behavior group included 3,725 students (91.7%). The most common source of information on the COVID-19 vaccine was TV/radio (75.0%), and the most common Social networking service (SNS) for COVID-19 information was Twitter (31.1%). Approximately 85% students believed in the efficacy of vaccination. Positive attitude was associated with female sex and watching an educational movie by the university. Concern about the vaccine was positively associated with information from acquaintances or Instagram, and negatively associated with information from Twitter, and watching the educational movie. The majority of students had positive beliefs and attitudes toward COVID-19 vaccination, and social media and educational movies produced by the university had a large influence on their attitude toward vaccination behavior. On the contrary, some students were anxious about vaccination. Promotion of educational movies on social media by the universities is an effective way to encourage vaccination among young people., Competing Interests: The authors have declared that no competing interests exist., (Copyright: © 2022 Sakamoto et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.) more...
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- 2022
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28. Combined results of three physical performance tests predict incident fracture independently of aBMD in community-dwelling elderly Japanese men: Fujiwara-kyo Osteoporosis Risk in Men (FORMEN) Cohort Study.
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Fujita Y, Iki M, Yura A, Harano A, Kouda K, Tamaki J, Sato Y, Tachiki T, Kajita E, Ishizuka R, Moon JS, Okamoto N, and Kurumatani N
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- Aged, Bone Density, Cohort Studies, Female, Follow-Up Studies, Hand Strength, Humans, Independent Living, Japan epidemiology, Male, Physical Functional Performance, Risk Factors, Osteoporosis complications, Osteoporosis epidemiology, Osteoporotic Fractures epidemiology, Osteoporotic Fractures etiology
- Abstract
Background: Several studies have examined the association between physical performance and fracture in women, but few such studies have targeted elderly men. This study aimed to determine whether the combined results of several physical performance tests can predict the subsequent incidence of fractures in elderly men after adjusting for confounding factors., Methods: Of the 2174 elderly men who participated in this study, 2012 completed the baseline study visit, including physical performance tests (walking speed, hand grip strength, and one-leg standing) and measurement of bone mineral density by dual-energy X-ray absorptiometry. Follow-up study visits were conducted five and ten years later, during which incident fractures were identified by detailed interviews. We excluded 140 men with diseases or who took medications known to affect bone metabolism at baseline, 185 with missing values for predictors and potential confounding factors, and one who did not participate in any of the follow-up study visits. The remaining 1686 men were analyzed. Each physical performance test was analyzed by quartiles. Poor performance was defined as belonging to the worst quartile of performance. The association between physical performance and fracture was assessed using Cox proportional hazards models., Results: We identified 175 clinical fractures (osteoporotic fracture: 77, major osteoporotic fracture: 48) in 1686 men during a mean follow-up period of 8.4 years. After adjusting for potential confounding factors including bone mineral density, men who performed poorly on all three physical performance tests had a 3.7-fold higher risk of osteoporotic fracture and a 6.6-fold higher risk of major osteoporotic fracture than men who did not perform poorly on any of the tests., Conclusions: Japanese elderly men who performed poorly on all three physical performance tests had a significantly higher risk of incident osteoporotic fracture independently of bone mineral density. The combined results of several physical performance tests may be useful for predicting incident fractures in elderly men., (Copyright © 2021 Elsevier Inc. All rights reserved.) more...
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- 2022
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29. Circulating osteocalcin levels were not significantly associated with the risk of incident type 2 diabetes mellitus in elderly Japanese men: The Fujiwara-kyo Osteoporosis Risk in Men (FORMEN) Cohort Study.
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Iki M, Yura A, Fujita Y, Kouda K, Tamaki J, Tachiki T, Kajita E, Iwaki H, Ishizuka R, Moon JS, Okamoto N, and Kurumatani N
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- Aged, Biomarkers, Cohort Studies, Cross-Sectional Studies, Humans, Japan epidemiology, Longitudinal Studies, Male, Osteocalcin, Diabetes Mellitus, Type 2 epidemiology, Osteoporosis
- Abstract
Introduction: Cross-sectional studies have shown that patients with type 2 diabetes mellitus (T2DM) have low circulating levels of osteocalcin (OC) and undercarboxylated OC (ucOC). This longitudinal study aimed to examine whether low OC or ucOC levels at baseline are associated with the risk of incident T2DM., Methods: We examined 1700 community-dwelling Japanese men (≥65 years) after excluding those with history of diseases (other than T2DM) or medications that affect bone and glucose metabolism. T2DM was defined as fasting plasma glucose (FPG) ≥126 mg/dl or glycated hemoglobin A
1c (HbA1c) ≥6.5%. Participants without prevalent T2DM at baseline were invited to follow-up surveys 5 and 10 years after baseline., Results: Among the participants, 309 with prevalent T2DM showed significantly lower serum OC and ucOC levels at baseline than those without. After excluding these participants, 46 and 57 participants with incident T2DM were identified in the first and second follow-up surveys, respectively. These participants did not show significantly different OC and ucOC levels at baseline relative to those without T2DM, although their FPG and HbA1c levels at baseline were significantly higher compared to those without incident T2DM. Increase in glycemic indices preceded decrease in OC and ucOC levels. OC and ucOC levels at baseline were not significantly associated with the risk of incident T2DM identified in the follow-up surveys., Conclusions: OC and ucOC levels at baseline were not significantly associated with the risk of incident T2DM. Our results do not support the findings of animal studies that ucOC is a hormone regulating glucose metabolism., (Copyright © 2021 Elsevier Inc. All rights reserved.) more...- Published
- 2021
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30. Pactacin is a novel digestive enzyme in teleosts.
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Kawaguchi M, Okazawa Y, Imafuku A, Nakano Y, Shimizu R, Ishizuka R, Jiang T, Nagasawa T, Hiroi J, and Yasumasu S
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- Amino Acid Sequence, Animals, Cloning, Molecular, Sequence Homology, Amino Acid, Enzyme Precursors biosynthesis, Enzyme Precursors genetics, Fish Proteins biosynthesis, Fish Proteins genetics, Gene Expression Regulation, Enzymologic, Metalloendopeptidases biosynthesis, Metalloendopeptidases genetics, Oryzias genetics, Oryzias metabolism, Pancreas enzymology, Smegmamorpha genetics, Smegmamorpha metabolism
- Abstract
Generally, animals extract nutrients from food by degradation using digestive enzymes. Trypsin and chymotrypsin, one of the major digestive enzymes in vertebrates, are pancreatic proenzymes secreted into the intestines. In this investigation, we report the identification of a digestive teleost enzyme, a pancreatic astacin that we termed pactacin. Pactacin, which belongs to the astacin metalloprotease family, emerged during the evolution of teleosts through gene duplication of astacin family enzymes containing six cysteine residues (C6astacin, or C6AST). In this study, we first cloned C6AST genes from pot-bellied seahorse (Hippocampus abdominalis) and analyzed their phylogenetic relationships using over 100 C6AST genes. Nearly all these genes belong to one of three clades: pactacin, nephrosin, and patristacin. Genes of the pactacin clade were further divided into three subclades. To compare the localization and functions of the three pactacin subclades, we studied pactacin enzymes in pot-bellied seahorse and medaka (Oryzias latipes). In situ hybridization revealed that genes of all three subclades were commonly expressed in the pancreas. Western blot analysis indicated storage of pactacin pro-enzyme form in the pancreas, and conversion to the active forms in the intestine. Finally, we partially purified the pactacin from digestive fluid, and found that pactacin is novel digestive enzyme that is specific in teleosts. more...
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- 2021
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31. Highly efficient titanosilicate catalyst Ti-MCM-68 prepared using a liquid-phase titanium source for the phenol oxidation.
- Author
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Inagaki S, Ishizuka R, Ikehara Y, Odagawa S, Asanuma K, Morimoto S, and Kubota Y
- Abstract
A highly efficient Ti-MCM-68 catalyst for phenol oxidation with H
2 O2 was prepared by a mild liquid-phase treatment for the first time. The key preparation procedures to excellent catalytic activity and high para -selectivity were the use of aqueous solutions of the Ti source and calcination at 650 °C prior to catalytic use., Competing Interests: There are no conflicts to declare., (This journal is © The Royal Society of Chemistry.) more...- Published
- 2021
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32. Development and validation of nutrient estimates based on a food-photographic record in Japan.
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Saeki K, Otaki N, Kitagawa M, Tone N, Takachi R, Ishizuka R, Kurumatani N, and Obayashi K
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- Humans, Japan, Meals, Reproducibility of Results, Energy Intake, Nutrients
- Abstract
Background: Previous studies have reported that estimates of portion size, energy, and macronutrients such as carbohydrates, proteins, and fat based on the food-photographic record closely correlate with directly weighed values. However, the correlation based on a large sample of the test meal with the evidence of many nutrients is yet to be determined. We conducted this study to assess the correlation and difference between the food-photographic record and weighed results for 44 nutrients based on a larger number of test meals than those in previous studies., Methods: We assessed the nutrients of test meals using a food-photographic record and direct weighing and compared the results of the two methods. Twenty participants prepared a total of 1163 test meals. Each participant cooked 28-29 different kinds of dishes. Five participants cooked the same dish with their own recipes. For the most commonly consumed 41 dishes, 20 participants served a meal with their usual portion size. For the remaining 73 dishes, five participants served a meal with their usual portion size. An independent researcher weighed each ingredient and calculated the nutrients of the test meals. The participants took photographs of the test meals using a digital camera. Two independent, trained analysts measured the longitudinal and transverse diameters of the food area on the photographs of the test meals, compared the portion size with the reference photographs, and calculated the nutrients based on a database that contained reference photographs., Results: Rank correlation coefficients between estimates from the food-photographic record of each test meal and weighed results were high for portion size (r = 0.93), energy (r = 0.93), protein (r = 0.90), fat (r = 0.92), and carbohydrate (r = 0.94), and those for the 44 nutrients ranged from 0.78 to 0.94. We found high reproducibility between the two analysts for all the nutrients (r > 0.90)., Conclusions: We found a high correlation and small difference between the food-photographic record method and weighed results of a large number of nutrients in many test meals. more...
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- 2020
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33. Management of Multiple Arteriovenous Malformations of the Small Bowel.
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Hirakawa M, Ishizuka R, Sato M, Hayasaka N, Ohnuma H, Murase K, Takada K, Ito T, Nobuoka T, Miyanishi K, Kobune M, Takemasa I, and Kato J
- Abstract
A 62-year-old Japanese female was referred to our hospital with gastrointestinal bleeding. Although small-bowel bleeding was suspected, no bleeding source was identified by enhanced computed tomography (CT), video capsule endoscopy (VCE), and double-balloon enteroscopy (DBE). Five years later, the patient had recurrent intermittent bloody stools with a significant decrease in hemoglobin levels. Although no active bleeding was observed on antegrade DBE, we detected a pulsatile submucosal uplift accompanied by a small red patch on the top of the uplift in the jejunum. Arteriovenous malformation (AVM) was suspected as the cause of small-bowel bleeding. Multiple-phase CT showed a number of small vascular ectasias during the arterial phase in the jejunum, and we confirmed the presence of multiple AVMs in the jejunum by selective angiography. To identify the location of the lesions and determine the minimal surgical margins, we performed intraoperative selective angiography with indocyanine green (ICG) injection. This technique allowed us to clearly observe the region and perform segmental small-bowel resection with minimal surgical margin. The patient reported that she has had no gastrointestinal bleeding at the two years follow-up visit., Competing Interests: The authors declare that they have no conflicts of interest., (Copyright © 2019 Masahiro Hirakawa et al.) more...
- Published
- 2019
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34. Effective charges of ionic liquid determined self-consistently through combination of molecular dynamics simulation and density-functional theory.
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Ishizuka R and Matubayasi N
- Abstract
A self-consistent scheme combining the molecular dynamics (MD) simulation and density functional theory (DFT) was recently proposed to incorporate the effects of the charge transfer and polarization of ions into non-poralizable force fields of ionic liquids for improved description of energetics and dynamics. The purpose of the present work is to analyze the detailed setups of the MD/DFT scheme by focusing on how the basis set, exchange-correlation (XC) functional, charge-fitting method or force field for the intramolecular and Lennard-Jones interactions affects the MD/DFT results of 1,3-dimethylimidazolium bis(trifluoromethylsulfonyl) imide ( [C1mim][NTf2]) and 1-ethyl-3-methylimidazolium glycinate ( [C2mim][Gly]). It was found that the double-zeta valence polarized or larger size of basis set is required for the convergence of the effective charge of the ion. The choice of the XC functional was further not influential as far as the generalized gradient approximation is used. The charge-fitting method and force field govern the accuracy of the MD/DFT scheme, on the other hand. We examined the charge-fitting methods of Blöchl, the iterative Hirshfeld (Hirshfeld-I), and REPEAT in combination with Lopes et al.'s force field and general AMBER force field. There is no single combination of charge fitting and force field that provides good agreements with the experiments, while the MD/DFT scheme reduces the effective charges of the ions and leads to better description of energetics and dynamics compared to the original force field with unit charges. © 2017 Wiley Periodicals, Inc., (© 2017 Wiley Periodicals, Inc.) more...
- Published
- 2017
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35. [A Case of Leiomyosarcoma Arising from the Ovarian Vein Preoperatively Diagnosed with Radiological Imaging].
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Tsuchiya H, Kawahara T, Kawai K, Chihara I, Tanaka K, Kimura T, Ikeda A, Yoshino T, Ishizuka R, Waku N, Kandori S, Kojima T, Joraku A, Nakano N, Nasu K, Minami M, and Nishiyama H
- Subjects
- Female, Humans, Leiomyosarcoma surgery, Middle Aged, Multimodal Imaging, Positron Emission Tomography Computed Tomography, Tomography, X-Ray Computed, Vascular Neoplasms surgery, Veins surgery, Leiomyosarcoma blood supply, Leiomyosarcoma diagnostic imaging, Ovary blood supply, Vascular Neoplasms diagnostic imaging, Vascular Neoplasms pathology, Veins diagnostic imaging, Veins pathology
- Abstract
A 54-year-old woman presented withtransient back pain. She was diagnosed withleiomyosarcoma of the inferior vena cava (IVC) by computed tomography (CT) and was referred to our hospital. Contrastenhanced CT revealed a mass (38×42 mm) located in the retroperitoneal space along the course of the right ovarian vein. The mass compressed the IVC into a crescent shape. A tumor thrombus was also found in the IVC. 18 F-fluoro-2-deoxy-D-glucose positron emission tomography (PET) revealed high uptake at the caudal side of the tumor. These radiological findings strongly suggested the diagnosis of leiomyosarcoma arising from the right ovarian vein. She underwent tumor resection with right nephrectomy, IVC resection, and IVC patch reconstruction without any notable events after surgery. Histopathological diagnosis was leiomyosarcoma arising from the ovarian vein, not from the IVC. Two months after the surgery, CT revealed multiple pulmonary metastases and a single liver metastasis. The patient was referred to another hospital for further treatment. She was treated with chemotherapy and was alive with disease at 14 months after the surgery. more...
- Published
- 2017
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36. Energetic Analysis of Adsorption and Absorption of Small Molecule to Nanodroplet of Water.
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Sayou M, Ishizuka R, and Matubayasi N
- Abstract
Adsorption and absorption were analyzed for nonpolar and polar solutes to a water droplet of nanometer size and to a planar slab. All-atom molecular dynamics simulation was performed, and the free energy change for bringing the solute to the water aggregate was computed over a wide range of temperature. It was seen in both the droplet and slab systems that the solute is preferably located at the surface, and the propensity of the nonpolar solute at the surface relative to the bulk was found to be larger in the droplet than in the slab. A molecular-sized curvature thus enhances the surface propensity of a nonpolar solute, whereas the curvature effect is weaker for polar one. The attractive and repulsive interactions of the solute with water were further analyzed, and the role of the repulsive interaction is discussed with respect to the stability of the surface-bound state. more...
- Published
- 2017
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37. Computing conformational free energy differences in explicit solvent: An efficient thermodynamic cycle using an auxiliary potential and a free energy functional constructed from the end points.
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Harris RC, Deng N, Levy RM, Ishizuka R, and Matubayasi N
- Subjects
- Molecular Conformation, Molecular Dynamics Simulation, Solvents chemistry, Dipeptides chemistry, Thermodynamics, Water chemistry, beta-Cyclodextrins chemistry
- Abstract
Many biomolecules undergo conformational changes associated with allostery or ligand binding. Observing these changes in computer simulations is difficult if their timescales are long. These calculations can be accelerated by observing the transition on an auxiliary free energy surface with a simpler Hamiltonian and connecting this free energy surface to the target free energy surface with free energy calculations. Here, we show that the free energy legs of the cycle can be replaced with energy representation (ER) density functional approximations. We compute: (1) The conformational free energy changes for alanine dipeptide transitioning from the right-handed free energy basin to the left-handed basin and (2) the free energy difference between the open and closed conformations of β-cyclodextrin, a "host" molecule that serves as a model for molecular recognition in host-guest binding. β-cyclodextrin contains 147 atoms compared to 22 atoms for alanine dipeptide, making β-cyclodextrin a large molecule for which to compute solvation free energies by free energy perturbation or integration methods and the largest system for which the ER method has been compared to exact free energy methods. The ER method replaced the 28 simulations to compute each coupling free energy with two endpoint simulations, reducing the computational time for the alanine dipeptide calculation by about 70% and for the β-cyclodextrin by > 95%. The method works even when the distribution of conformations on the auxiliary free energy surface differs substantially from that on the target free energy surface, although some degree of overlap between the two surfaces is required. © 2016 Wiley Periodicals, Inc., (© 2016 Wiley Periodicals, Inc.) more...
- Published
- 2017
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38. Drastic Compensation of Electronic and Solvation Effects on ATP Hydrolysis Revealed through Large-Scale QM/MM Simulations Combined with a Theory of Solutions.
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Takahashi H, Umino S, Miki Y, Ishizuka R, Maeda S, Morita A, Suzuki M, and Matubayasi N
- Subjects
- Electrons, Energy Transfer, Hydrolysis, Molecular Dynamics Simulation, Quantum Theory, Thermodynamics, Adenosine Triphosphate chemistry, Solvents chemistry, Water chemistry
- Abstract
Hydrolysis of adenosine triphosphate (ATP) is the "energy source" for a variety of biochemical processes. In the present work, we address key features of ATP hydrolysis: the relatively moderate value (about -10 kcal/mol) of the standard free energy, ΔG
hyd , of reaction and the insensitivity of ΔGhyd to the number of excess electrons on ATP. We conducted quantum mechanical/molecular mechanical simulation combined with the energy-representation theory of solutions to analyze the electronic-state and solvation contributions to ΔGhyd . It was revealed that the electronic-state contribution in ΔGhyd is largely negative (favorable) upon hydrolysis, due to the reduction of electrostatic repulsion accompanying the breakage of the P-O bond. In contrast, the solvation effect was found to be strongly more favorable on the reactant side. Thus, we showed that a drastic compensation of the two opposite effects takes place, leading to the modest value of ΔGhyd at each number of excess electrons examined. The computational analyses were also conducted for pyrophosphate ions (PPi), and the parallelism between the ATP and PPi hydrolyses was confirmed. Classical molecular dynamics simulation was further carried out to discuss the effect of the solvent environment; the insensitivity of ΔGhyd to the number of excess electrons was seen to hold in solvent water and ethanol. more...- Published
- 2017
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39. The clinical presentation and favorable prognosis of patients with isolated metachronous brain metastasis from germ cell tumors.
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Kawahara T, Kawai K, Yoshino T, Ikeda A, Ishizuka R, Kandori S, Takaoka EI, Kojima T, Joraku A, Suetomi T, Miyazaki J, and Nishiyama H
- Abstract
Objective: We conducted the present study to elucidate the clinical presentation, treatment outcomes and risk factors for the development of metachronous brain metastasis at a single progressive disease site, the so-called isolated brain metastasis, in patients with testicular germ cell tumors., Methods: To identify metachronous brain metastasis in a timely manner, brain imaging was performed when the re-elevation of tumor markers was observed during chemotherapy, even in patients who were free from central nervous system symptoms. The medical records of 147 patients with metastatic germ cell tumors who were treated between 1991 and 2015 were retrospectively reviewed., Results: Eight (5.4%) of the 147 patients presented synchronous brain metastasis. Of these, five patients suffered from metachronous brain metastasis relapse. An additional nine patients developed metachronous brain metastasis during or after chemotherapy. Ten of the 14 patients with metachronous brain metastasis did not have central nervous system symptoms. Eight (57%) patients had isolated brain metastasis. Ten patients underwent multimodal treatments, predominantly chemotherapy and radiotherapy. The 3-year overall survival of all 14 patients was 34.6%, but that of the patients with isolated brain metastasis was high as 66.7%. The development of metachronous brain metastasis was associated with a choriocarcinoma element at the primary site and an human chorionic gonadotropin level of >50 000 IU/L and brain metastasis at the initial diagnosis., Conclusions: In our series, we identified isolated brain metastasis in 57% of the metachronous brain metastasis patients. The monitoring of tumor markers and appropriate brain imaging are mandatory for the diagnosis of isolated brain relapse, which is associated with a higher rate of long-term survival., (© The Author 2016. Published by Oxford University Press. All rights reserved. For Permissions, please email: journals.permissions@oup.com.) more...
- Published
- 2016
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40. Erratum to: Reliability and validity of Japanese versions of KIDSCREEN-27 and KIDSCREEN-10 questionnaires.
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Nezu S, Iwasaka H, Saeki K, Obayashi K, Ishizuka R, Goma H, Furuichi Y, and Kurumatani N
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- 2016
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41. Energetics of nonpolar and polar compounds in cationic, anionic, and nonionic micelles studied by all-atom molecular dynamics simulation combined with a theory of solutions.
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Date A, Ishizuka R, and Matubayasi N
- Abstract
Energetic analysis was conducted for nonpolar and polar solutes bound in a cationic micelle of dodecyl trimethyl ammonium bromide (DTAB), an anionic micelle of sodium dodecyl sulfate (SDS), and a nonionic micelle of tetraethylene glycol monododecyl ether (Brij30). All-atom molecular dynamics simulation was performed, and the free energies of binding the solutes in the hydrophobic-core and headgroup regions of the micelles were computed using the energy-representation method. It was found in all the micelles examined that aromatic naphthalene is preferably located more outward than aliphatic propane and that the polar solutes are localized at the interface of the hydrophobic and hydrophilic regions. The roles of the surfactant and water were then elucidated by decomposing the free energy into the contributions from the respective species. Water was observed to play a decisive role in determining the binding location of the solute, while the surfactant was found to be more important for the overall stabilization of the solute within the micelle. The effects of attractive and repulsive interactions of the solute with the surfactant and water were further examined, and their competition was analyzed in connection with the preferable location of the solute in the micellar system. more...
- Published
- 2016
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42. Reliability and validity of Japanese versions of KIDSCREEN-27 and KIDSCREEN-10 questionnaires.
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Nezu S, Iwasaka H, Saeki K, Obayashi K, Ishizuka R, Goma H, Furuichi Y, and Kurumatani N
- Subjects
- Adolescent, Adult, Child, Cross-Sectional Studies, Female, Humans, Japan, Male, Middle Aged, Parents, Quality of Life, Reproducibility of Results, Psychometrics methods, Surveys and Questionnaires standards
- Abstract
Objective: This study aimed to assess the reliability and validity of Japanese versions of the KIDSCREEN-27 (J-KIDSCREEN-27) and KIDSCREEN-10 (J-KIDSCREEN-10) questionnaires, which are shorter versions of the KIDSCREEN-52 (J-KIDSCREEN-52)., Methods: The present analyses are based on a pre-existing dataset of the J-KIDSCREEN-52 validation study, including 1564 children and adolescents aged 8-18 years and their 1326 parents. All were asked to complete the J-KIDSCREEN and Pediatric Quality of Life Inventory (PedsQL) questionnaires. Test-retest reliability was assessed with Intraclass Correlation Coefficients (ICCs) in a one-way random effects model, and internal consistency reliability was measured using Cronbach's alpha coefficients. Agreement between child and parent scores was evaluated using ICCs in a two-way mixed effects model. To assess concurrent validity, a sub-sample of 535 parents evaluated their child's mental health status using the Strengths and Difficulties Questionnaire (SDQ)., Results: For children, test-retest ICCs were ≥0.60 and Cronbach's alpha ≥0.70 for every dimension of both instruments. Correlations of corresponding dimensions between the J-KIDSCREEN-27 or -10 and the PedsQL were acceptable. For parents, test-retest ICCs were ≥0.60, Cronbach's alpha ≥0.70, and ICCs between child and parent scores ≥0.41 in every dimension of both instruments. In multivariate logistic regression models, after adjusting for confounders, lower health-related QOL in every dimension of both instruments, except Physical Well-being, was significantly associated with higher odds ratios for borderline and clinical ranges of the SDQ., Conclusion: The child/adolescent and parent/proxy versions of the J-KIDSCREEN-27 and J-KIDSCREEN-10 demonstrated acceptable levels of reliability and validity. more...
- Published
- 2016
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43. Interaction-component analysis of the hydration and urea effects on cytochrome c.
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Yamamori Y, Ishizuka R, Karino Y, Sakuraba S, and Matubayasi N
- Subjects
- Molecular Dynamics Simulation, Solvents chemistry, Water chemistry, Cytochromes c chemistry, Urea chemistry
- Abstract
Energetics was analyzed for cytochrome c in pure-water solvent and in a urea-water mixed solvent to elucidate the solvation effect in the structural variation of the protein. The solvation free energy was computed through all-atom molecular dynamics simulation combined with the solution theory in the energy representation, and its correlations were examined over sets of protein structures against the electrostatic and van der Waals components in the average interaction energy of the protein with the solvent and the excluded-volume component in the solvation free energy. It was observed in pure-water solvent that the solvation free energy varies in parallel to the electrostatic component with minor roles played by the van der Waals and excluded-volume components. The effect of urea on protein structure was then investigated in terms of the free-energy change upon transfer of the protein solute from pure-water solvent to the urea-water mixed solvent. The decomposition of the transfer free energy into the contributions from urea and water showed that the urea contribution is partially canceled by the water contribution and governs the total free energy of transfer. When correlated against the change in the solute-solvent interaction energy upon transfer and the corresponding changes in the electrostatic, van der Waals, and excluded-volume components, the transfer free energy exhibited strong correlations with the total change in the solute-solvent energy and its van der Waals component. The solute-solvent energy was decomposed into the contributions from the protein backbone and side chain, furthermore, and neither of the contributions was seen to be decisive in the correlation to the transfer free energy. more...
- Published
- 2016
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44. Self-Consistent Determination of Atomic Charges of Ionic Liquid through a Combination of Molecular Dynamics Simulation and Density Functional Theory.
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Ishizuka R and Matubayasi N
- Abstract
A self-consistent scheme is developed to determine the atomic partial charges of ionic liquid. Molecular dynamics (MD) simulation was conducted to sample a set of ion configurations, and these configurations were subject to density functional theory (DFT) calculations to determine the partial charges. The charges were then averaged and used as inputs for the subsequent MD simulation, and MD and DFT calculations were repeated until the MD results are not altered any more. We applied this scheme to 1,3-dimethylimidazolium bis(trifluoromethylsulfonyl) imide ([C1mim][NTf2]) and investigated its structure and dynamics as a function of temperature. At convergence, the average ionic charges were ±0.84 e at 350 K due to charge transfer among ions, where e is the elementary charge, while the reduced ionic charges do not affect strongly the density of [C1mim][NTf2] and radial distribution function. Instead, major effects are found on the energetics and dynamics, with improvements of the overestimated heat of vaporization and the too slow motions of ions observed in MD simulations using commonly used force fields. more...
- Published
- 2016
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45. Correlation analysis for heat denaturation of Trp-cage miniprotein with explicit solvent.
- Author
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Kamo F, Ishizuka R, and Matubayasi N
- Subjects
- Protein Conformation, Solvents chemistry, Hot Temperature, Molecular Dynamics Simulation, Peptides chemistry, Protein Denaturation, Water chemistry
- Abstract
Energetics was analyzed for Trp-cage miniprotein in water to elucidate the solvation effect in heat denaturation. The solvation free energy was computed for a set of protein structures at room and high temperatures with all-atom molecular dynamics simulation combined with the solution theory in the energy representation, and its correlations were investigated against the intramolecular (structural) energy of the protein and the average interaction energy of the protein with the solvent water. It was observed both at room and high temperatures that the solvation free energy is anticorrelated to the structural energy and varies in parallel to the electrostatic component of the protein-water interaction energy without correlations to the van der Waals and excluded-volume components. When the set of folded structures sampled at room temperature was compared with the set of unfolded ones at high temperature, it was found that the preference order of the two sets is in correspondence to the van der Waals and excluded-volume components in the sum of the protein intramolecular and protein-water intermolecular interactions and is not distinguished by the electrostatic component., (© 2015 The Protein Society.) more...
- Published
- 2016
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46. Combination of Root Surface Modification with BMP-2 and Collagen Hydrogel Scaffold Implantation for Periodontal Healing in Beagle Dogs.
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Kato A, Miyaji H, Ishizuka R, Tokunaga K, Inoue K, Kosen Y, Yokoyama H, Sugaya T, Tanaka S, Sakagami R, and Kawanami M
- Abstract
Unlabelled: Objective : Biomodification of the root surface plays a major role in periodontal wound healing. Root surface modification with bone morphogenetic protein (BMP) stimulates bone and cementum-like tissue formation; however, severe ankylosis is simultaneously observed. Bio-safe collagen hydrogel scaffolds may therefore be useful for supplying periodontal ligament cells and preventing ankylosis. We examined the effects of BMP modification in conjunction with collagen hydrogel scaffold implantation on periodontal wound healing in dogs., Material and Methods: The collagen hydrogel scaffold was composed of type I collagen sponge and collagen hydrogel. One-wall infrabony defects (5 mm in depth, 3 mm in width) were surgically created in six beagle dogs. In the BMP/Col group, BMP-2 was applied to the root surface (loading dose; 1 µg/µl), and the defects were filled with collagen hydrogel scaffold. In the BMP or Col group, BMP-2 coating or scaffold implantation was performed. Histometric parameters were evaluated at 4 weeks after surgery., Results: Single use of BMP stimulated formation of alveolar bone and ankylosis. In contrast, the BMP/Col group frequently enhanced reconstruction of periodontal attachment including cementum-like tissue, periodontal ligament and alveolar bone. The amount of new periodontal ligament in the BMP/Col group was significantly greater when compared to all other groups. In addition, ankylosis was rarely observed in the BMP/Col group., Conclusion: The combination method using root surface modification with BMP and collagen hydrogel scaffold implantation facilitated the reestablishment of periodontal attachment. BMP-related ankylosis was suppressed by implantation of collagen hydrogel. more...
- Published
- 2015
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47. Energetic contributions from the cation and anion to the stability of carbon dioxide dissolved in imidazolium-based ionic liquids.
- Author
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Ishizuka R, Matubayasi N, Tu KM, and Umebayashi Y
- Abstract
Cation and anion effects were investigated in the energetics of CO2 solvation in room-temperature ionic liquids. The solvation free energy in 1-n-butyl-3-methylimidazolium bis(trifluoromethylsulfonyl)imide ([C4mim][NTf2]) was calculated with three types of force fields by molecular dynamics simulations combined with the energy-representation (ER) method, and the interaction responsible for the CO2 stability was examined in terms of the average sum of the solute-solvent interaction energy and its electrostatic and van der Waals components. A key role of the van der Waals component was found for the cation contribution, and the electrostatic component was seen to be minor in the anion contribution. The solvation free energy of CO2 was also investigated in ionic liquids of the form 1-n-alkyl-3-methylimidazolium bis(trifluoromethylsulfonyl)imide ([Cnmim][NTf2]) with varying alkyl-chain lengths of n = 4, 6, 8, and 12 by the ER method. The free energy was found to depend weakly on n, in agreement with experiments, and the cation and anion contributions and the electrostatic and van der Waals interaction components acted similarly at all values of n examined. The alkyl-chain length was found not to affect the local structure around CO2 strongly, and its effect on the interaction energy with CO2 appeared mainly through the bulk density. more...
- Published
- 2015
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48. [Effect of Water Intake on Allergy-like Events Associated with Non-ionic Iodine Contrast Agents].
- Author
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Motoi R, Yano I, Ozaki J, Hokoyama K, Yamamoto T, Fukatsu S, Ishizuka R, Matsumura Y, Taniguchi M, Higashimura K, and Matsubara K
- Subjects
- Adult, Aged, Drug Hypersensitivity epidemiology, Female, Gastrointestinal Diseases epidemiology, Gastrointestinal Diseases etiology, Gastrointestinal Diseases prevention & control, Hospitals, University statistics & numerical data, Humans, Incidence, Japan epidemiology, Male, Middle Aged, Respiratory Tract Diseases epidemiology, Respiratory Tract Diseases etiology, Respiratory Tract Diseases prevention & control, Seasons, Time Factors, Contrast Media adverse effects, Drinking physiology, Drug Hypersensitivity etiology, Drug Hypersensitivity prevention & control, Iodine Compounds adverse effects
- Abstract
The use of iodine contrast agents occasionally causes serious allergic symptoms including anaphylaxis. At Kyoto University Hospital to prevent nephropathy we began recommending water intake before and after administration of iodine contrast agents in September 2012. In the present study we investigated the effect of water intake on the incidence of allergy-like events after the use of non-ionic iodine contrast agents. We extracted the occurrence of allergy-like events from the incident report system in our hospital from January 2011 to September 2014, and classified these events into the following 3 grades: 1+ (follow-up); 2+ (medication treatment); and 3+ (hospitalization). The allergy-like incidence rate was calculated for subsequent evaluation according to season and water intake. Allergy-like events significantly decreased from 0.49% before the recommendation of water intake to 0.26% at 1 year and 0.20% at 2 years after implementing the recommendation. The incidence of allergy-like events was significantly higher in summer than in winter before water intake was recommended. After implementing the recommendation, the value for summer significantly decreased to an incidence similar to that of winter. Respiratory and gastrointestinal allergy-like symptoms were dramatically decreased after implementing the recommendation. Water intake may be useful for preventing allergy-like events associated with non-ionic iodine contrast agents, especially during the summer. more...
- Published
- 2015
- Full Text
- View/download PDF
49. Reliability and validity of the Japanese version of the KIDSCREEN-52 health-related quality of life questionnaire for children/adolescents and parents/proxies.
- Author
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Nezu S, Iwasaka H, Saeki K, Ishizuka R, Goma H, Okamoto N, Makino H, Tanimura M, Yoshizaki K, Obayashi K, and Kurumatani N
- Subjects
- Adolescent, Age Distribution, Child, Cross-Sectional Studies, Factor Analysis, Statistical, Female, Humans, Japan, Male, Parents, Reproducibility of Results, Schools, Sex Distribution, Students, Quality of Life, Surveys and Questionnaires standards
- Abstract
Objectives: The present study aimed to assess the reliability and validity of the Japanese version of KIDSCREEN-52 (J-KIDSCREEN-52), a generic questionnaire used to assess health-related quality of life (HRQOL) among children/adolescents and parents/proxies., Methods: We conducted a school-based study, in which 1564 children and adolescents aged 8-18 years and their 1326 parents participated from five schools. They were asked to complete two questionnaires (the J-KIDSCREEN-52 and the Pediatric Quality of Life Inventory (PedsQL)), and the Oslo 3-Item Social Support (OSS-3) scale. Internal consistency reliability was measured using the Cronbach's alpha coefficient. Test-retest reliability was assessed by the Intraclass Correlation Coefficient (ICC) in the one-way random effects model in sub-samples taken approximately three to four weeks apart. Agreement between the ratings of the child and parent was evaluated using the ICC in the two-way mixed effects model among 681 pairs., Results: For the overall sample, Cronbach's alpha values of 10 dimensions were ≥0.70, except for one dimension. Test-retest ICCs were ≥0.60 for nearly all dimensions. Correlation coefficients between the J-KIDSCREEN-52 and the PedsQL dimensions indicated a reasonable convergent validity. Parent ratings corresponded well with child ratings (ICC = 0.38-0.62). Statistically significant differences in mean T scores were dependent on gender for seven dimensions, age group for all dimensions, and health status for two dimensions., Conclusions: The J-KIDSCREEN-52 questionnaires child/adolescent and parent/proxy versions demonstrated acceptable levels of reliability and validity. more...
- Published
- 2015
- Full Text
- View/download PDF
50. Spatial-decomposition analysis of electrical conductivity in ionic liquid.
- Author
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Tu KM, Ishizuka R, and Matubayasi N
- Abstract
The electrical conductivity of room temperature ionic liquid (IL) is investigated with molecular dynamics simulation. A trajectory of 1 μs in total is analyzed for the ionic liquid [C4mim][NTf2] (1-n-butyl-3-methylimidazolium bis(trifluoromethylsulfonyl)imide, and the anion is also called TFSI or TFSA), and the ion motions are examined in direct connection to the conductivity within the framework formulated previously [K.-M. Tu, R. Ishizuka, and N. Matubayasi, J. Chem. Phys. 141, 044126 (2014)]. As a transport coefficient, the computed electrical conductivity is in fair agreement with the experiment. The conductivity is then decomposed into the autocorrelation term of Nernst-Einstein form and the cross-correlation term describing the two-body motions of ions, and the cross-correlation term is further decomposed spatially to incorporate the structural insights on ion configurations into the dynamic picture. It is observed that the ion-pair contribution to the conductivity is not spatially localized and extends beyond the first coordination shell. The extent of localization of the cross-correlation effect in the conductivity is in correspondence to that of the spatial correlation represented by radial distribution function, which persists over nanometer scale. more...
- Published
- 2014
- Full Text
- View/download PDF
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