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112 results on '"Ishikawa, Katsutoshi"'

6. Development of Flutter Analysis Tool Using Next-Generation CFD (Computational Fluid Dynamics) Algorithms

Author

Yumitori, Takaaki, Ishikawa, Katsutoshi, Takenaka, Keizo, and Azuma, Toshihiko

Subjects
flutter, CFD, cartesian mesh
Abstract

First International Symposium on Flutter and its Application (May 15-17, 2016. Mielparque-tokyo), Minato-ku, Tokyo, Japan, Both high accuracy and high efficiency are strongly required for flutter analysis. To achieve these requirements, MHI developed a flutter analysis tool based on the BCM (Building-Cube Method), which is a next-generation CFD (Computational Fluid Dynamics) algorithm using an advanced Cartesian mesh method. The BCM has several advantages such as rapid mesh generation, simple data structure for handling complex geometry, adaptive space-time resolution, and easy parallelization with ideal load balance. The developed tool is validated with the NASA ARROW WING model. Computational time is greatly reduced to less than one-third by applying adaptive inner time iteration technique. Predicted flutter boundary agrees well with experimental data., 形態: カラー図版あり, Physical characteristics: Original contains color illustrations, 資料番号: AA1630046044, レポート番号: JAXA-SP-16-008E

Published
2017

9. 極低温キャビテーションモデリングの多面的研究と開発への応用に向けて

Author

TANI, Naoki, ITO, Yu, Tsuda, Shinichi, Ishikawa, Katsutoshi, Himeno, Takehiro, Kawatsu, Kaname, and Yamanishi, Nobuhiro

Abstract

東京大学ロケットエンジンモデリングラボラトリー(JAXA社会連携講座)シンポジウム (2012年9月26日-27日. 東京大学), 東京, Symposium on Rocket Engine Modeling Laboratory, The University of Tokyo (JAXA public relations lecture): Advances in Rocket Engine Modeling and Simulation, and its Future (September 26-27, 2012. The University of Tokyo), Tokyo Japan, 形態: カラー図版あり, 共催: 東京大学ロケットエンジンモデリングラボラトリー(JAXA社会連携講座), 宇宙航空研究開発機構, Physical characteristics: Original contains color illustrations, Meeting sponsors: Rocket Engine Modeling Laboratory, The University of Tokyo (JAXA public relations lecture), JAXA's Engineering Degital Innovation Center, Japan Aerospace Exploration Agency (JAXA)(JEDI), Translation title: Approach toward diversified research and application of development of cryogenic cavitating flow modeling, 資料番号: AA0061924004, レポート番号: JAXA-SP-12-014

Published
2013

10. 極低温キャビテーションモデリングの多面的研究と開発への応用に向けて

Author

谷, 直樹, 伊藤, 優, 津田, 伸一, 石川, 勝利, 姫野, 武洋, 河津, 要, 山西, 伸宏, Tani, Naoki, Ito, Yutaka, Tsuda, Shinichi, Ishikawa, Katsutoshi, Himeno, Takehiro, Kawatsu, Kaname, Yamanishi, Nobuhiro, 谷, 直樹, 伊藤, 優, 津田, 伸一, 石川, 勝利, 姫野, 武洋, 河津, 要, 山西, 伸宏, Tani, Naoki, Ito, Yutaka, Tsuda, Shinichi, Ishikawa, Katsutoshi, Himeno, Takehiro, Kawatsu, Kaname, and Yamanishi, Nobuhiro

Abstract

形態: カラー図版あり, 会議情報: 東京大学ロケットエンジンモデリングラボラトリー(JAXA社会連携講座)シンポジウム (2012年9月26日-27日. 東京大学), 東京, 共催: 東京大学ロケットエンジンモデリングラボラトリー(JAXA社会連携講座), 宇宙航空研究開発機構, Physical characteristics: Original contains color illustrations, Meeting Information: Symposium on Rocket Engine Modeling Laboratory, The University of Tokyo (JAXA public relations lecture): Advances in Rocket Engine Modeling and Simulation, and its Future (September 26-27, 2012. The University of Tokyo), Tokyo Japan, Meeting sponsors: Rocket Engine Modeling Laboratory, The University of Tokyo (JAXA public relations lecture), JAXA's Engineering Degital Innovation Center, Japan Aerospace Exploration Agency (JAXA)(JEDI), Translation title: Approach toward diversified research and application of development of cryogenic cavitating flow modeling

Published
2015

11. 液体ロケットの推進薬タンクにおけるスロッシングと圧力変動予測へ向けた取組み

Author

Himeno, Takehiro, Nonaka, Satoshi, Naruo, Yoshihiro, Umemura, Yutaka, Ishikawa, Katsutoshi, Uzawa, Seiji, Watanabe, Toshinori, Sugimori, Daizo, Kinebuchi, Kiyoshi, and Okita, Koichi

Abstract

平成23年度宇宙輸送シンポジウム（2012年1月19日-20日. 宇宙航空研究開発機構宇宙科学研究所（JAXA)（ISAS)）, 相模原市, 神奈川県, Space Transportation Symposium FY2011 (January 19-20, 2012. Institute of Space and Astronautical Science, Japan Aerospace Exploration Agency (JAXA)(ISAS)), Sagamihara, Kanagawa Japan, 著者人数: 11名, 資料番号: SA6000003016, レポート番号: STCP-2011-016

Published
2012

15. Investigation on Pressure Change Induced by Cryogenic Sloshing

Author

Himeno, Takehiro, primary, Ishikawa, Katsutoshi, additional, Umemura, Yutaka, additional, Inoue, Chihiro, additional, Watanabe, Toshinori, additional, Nonaka, Satoshi, additional, Naruo, Yoshihiro, additional, Inatani, Yoshifumi, additional, Kinefuchi, Kiyoshi, additional, Sugimori, Daizo, additional, and Okita, Koichi, additional

Published
2012
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17. Heat Exchange and Pressure Drop Enhanced by Sloshing

Author

Himeno, Takehiro, primary, Sugimori, Daizo, additional, Ishikawa, Katsutoshi, additional, Umemura, Yutaka, additional, Uzawa, Seiji, additional, Inoue, Chihiro, additional, Watanabe, Toshinori, additional, Nonaka, Satoshi, additional, Naruo, Yoshihiro, additional, Inatani, Yoshifumi, additional, Kinefuchi, Kiyoshi, additional, Yamashiro, Ryoma, additional, Morito, Toshiki, additional, and Okita, Koichi, additional

Published
2011
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21. Identification of -type subunits of the Xenopus 20S proteasome and analysis of their changes during the meiotic cell cycle

Author

Wakata, Yuka, Tokumoto, Mika, Horiguchi, Ryo, Ishikawa, Katsutoshi, Nagahama, Yoshitaka, and Tokumoto, Toshinobu

Subjects
Proteasome Endopeptidase Complex, DNA, Complementary, Xenopus, Cell Cycle, Immunoblotting, Molecular Sequence Data, lcsh:Animal biochemistry, Antibodies, Monoclonal, Cell Differentiation, lcsh:Biochemistry, Meiosis, Protein Subunits, Cytosol, Oocytes, Animals, Humans, Electrophoresis, Gel, Two-Dimensional, lcsh:QD415-436, Amino Acid Sequence, Cloning, Molecular, Protein Processing, Post-Translational, lcsh:QP501-801, Research Article
Abstract

Background The 26S proteasome is the proteolytic machinery of the ubiquitin-dependent proteolytic system responsible for most of the regulated intracellular protein degradation in eukaryotic cells. Previously, we demonstrated meiotic cell cycle dependent phosphorylation of α4 subunit of the 26S proteasome. In this study, we analyzed the changes in the spotting pattern separated by 2-D gel electrophoresis of α subunits during Xenopus oocyte maturation. Results We identified cDNA for three α-type subunits (α1, α5 and α6) of Xenopus, then prepared antibodies specific for five subunits (α1, α3, α5, α6, and α7). With these antibodies and previously described monoclonal antibodies for subunits α2 and α4, modifications to all α-type subunits of the 26S proteasome during Xenopus meiotic maturation were examined by 2D-PAGE. More than one spot for all subunits except α7 was identified. Immunoblot analysis of 26S proteasomes purified from immature and mature oocytes showed a difference in the blots of α2 and α4, with an additional spot detected in the 26S proteasome from immature oocytes (in G2-phase). Conclusions Six of α-type subunits of the Xenopus 26S proteasome are modified in Xenopus immature oocytes and two subunits (α2 and α4) are modified meiotic cell cycle-dependently.

Published
2004

45. Identification of α-type subunits of the Xenopus 20S proteasome and analysis of their changes during the meiotic cell cycle.

Author

Wakata, Yuka, Tokumoto, Mika, Horiguchi, Ryo, Ishikawa, Katsutoshi, Nagahama, Yoshitaka, and Tokumoto, Toshinobu

Subjects
XENOPUS, CELL cycle, PROTEINS, ELECTROPHORESIS, OVUM
Abstract

Background: The 26S proteasome is the proteolytic machinery of the ubiquitin-dependent proteolytic system responsible for most of the regulated intracellular protein degradation in eukaryotic cells. Previously, we demonstrated meiotic cell cycle dependent phosphorylation of α4 subunit of the 26S proteasome. In this study, we analyzed the changes in the spotting pattern separated by 2-D gel electrophoresis of a subunits during Xenopus oocyte maturation. Results: We identified cDNA for three α-type subunits (α1, α5 and α6) of Xenopus, then prepared antibodies specific for five subunits (α1, α3, α5, α6, and α7). With these antibodies and previously described monoclonal antibodies for subunits α2 and α4, modifications to all α-type subunits of the 26S proteasome during Xenopus meiotic maturation were examined by 2D-PAGE. More than one spot for all subunits except α7 was identified. Immunoblot analysis of 26S proteasomes purified from immature and mature oocytes showed a difference in the blots of α2 and α4, with an additional spot detected in the 26S proteasome from immature oocytes (in G2-phase). Conclusions: Six of α-type subunits of the Xenopus 26S proteasome are modified in Xenopus immature oocytes and two subunits (α2 and α4) are modified meiotic cell cycle-dependently. [ABSTRACT FROM AUTHOR]

Published
2004
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46. Regulated interaction between polypeptide chain elongation factor-1 complex with the 26S proteasome during Xenopus oocyte maturation.

Author

Tokumoto, Toshinobu, Kondo, Ayami, Miwa, Junko, Horiguchi, Ryo, Tokumoto, Mika, Yoshitaka, Nagahama, Okida, Noriyuki, and Ishikawa, Katsutoshi

Subjects
PROTEIN-protein interactions, PEPTIDES, XENOPUS, OOGENESIS, IMMUNOGLOBULINS, ESCHERICHIA coli, BACTERIAL genetics, PHOSPHORYLATION
Abstract

Background: During Xenopus oocyte maturation, the amount of a 48 kDa protein detected in the 26S proteasome fraction (p48) decreased markedly during oocyte maturation to the low levels seen in unfertilized eggs. The results indicate that the interaction of at least one protein with the 26S proteasome changes during oocyte maturation and early development. An alteration in proteasome function may be important for the regulation of developmental events, such as the rapid cell cycle, in the early embryo. In this study, we identified p48. Results: p48 was purified by conventional column chromatography. The resulting purified fraction contained two other proteins with molecular masses of 30 (p30) and 37 (p37) kDa. cDNAs encode elongation factor-1γ and δ were obtained by an immuno-screening method using polyclonal antibodies against purified p48 complex, which recognized p48 and p37. N-terminal amino acid sequence analysis of p30 revealed that it was identical to EF-1β. To identify the p48 complex bound to the 26S proteasome as EF-1βγδ, antibodies were raised against the components of purified p48 complex. Recombinant EF-1 β,γ and δ were expressed in Escherichia coli, and an antibody was raised against purified recombinant EF-1γ. Cross-reactivity of the antibodies toward the p48 complex and recombinant proteins showed it to be specific for each component. These results indicate that the p48 complex bound to the 26S proteasome is the EF-1 complex. MPF phosphorylated EF-1γ was shown to bind to the 26S proteasome. When EF-1γ is phosphorylated by MPF, the association is stabilized. Conclusion: p48 bound to the 26S proteasome is identified as the EF-1γ. EF-1 complex is associated with the 26S proteasome in Xenopus oocytes and the interaction is stabilized by MPF-mediated phosphorylation. [ABSTRACT FROM AUTHOR]

Published
2003

48. The ter/ter Gonadal Somatic Cells Cause Apoptosis in ter/ter Primordial Germ Cells (PGCs) with Normal Survivability and Proliferation Ability in the Mouse: Evidence from PGC-Somatic Cell 'Exchange-Co-Culture.'

Author

Takabayashi, Shuji, Nozaki, Masami, Ishikawa, Katsutoshi, and Noguchi, Motoko

Subjects
GERM cells, GONADS, ZOOLOGY
Abstract

Provides information on a study which examined the survival and proliferation in ter/ter primordial germ cells (PGC) and the deficiency caused by ter/ter gonadal somatic cells in vitro. Materials and methods of the study; Results of the study; Discussion of the results.

Published
2001
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