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1. From intestine to beyond: Salmonella entry factors display distinct transcription pattern upon infection in murine models

Author

Michaël Koczerka, Isabelle Lantier, Marie Morillon, Justine Deperne, Camille D. Clamagirand, Isabelle Virlogeux-Payant, and Olivier Grépinet

Subjects
Salmonella, invasion, T3SS-1, invasins, murine models, in vivo imaging, Biology (General), QH301-705.5
Abstract

The infectious process of bacteria of the genus Salmonella requires the finely regulated use of various virulence factors. Among them, the type 3 secretion system-1 (T3SS-1) and the Rck and PagN invasins are involved in the internalization of the pathogen within eukaryotic cells, but their precise role in the host and in the pathogenic process is still poorly understood. In this study, we aimed to determine the kinetics of expression of these entry factors in a typhoid fever-like and a gastroenteritis model in mice by in vivo imaging using bioluminescent Salmonella Typhimurium reporter strains carrying chromosomal transcriptional fusions. Only pagN and T3SS-1 transcription has been clearly identified. Independently of the pathological model, the caecum was identified as the main transcription site of both pagN and the T3SS-1-encoding gene both at early and late stages of the infection. An intense transcription of pagN was also observed in deep organs in the typhoid fever-like model, while that of T3SS-1 remained quite sporadic in these organs, and mainly focused on the intestine all along the infection. This work will help to understand the respective role of these entry factors at the cellular level in the pathogenesis of Salmonella in vivo.

Published
2024
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2. In vivo imaging reveals novel replication sites of a highly oncogenic avian herpesvirus in chickens.

Author

Isabelle Lantier, Corentin Mallet, Laurent Souci, Thibaut Larcher, Andele M Conradie, Katia Courvoisier, Sascha Trapp, David Pasdeloup, Benedikt B Kaufer, and Caroline Denesvre

Subjects
Immunologic diseases. Allergy, RC581-607, Biology (General), QH301-705.5
Abstract

In vivo bioluminescence imaging facilitates the non-invasive visualization of biological processes in living animals. This system has been used to track virus infections mostly in mice and ferrets; however, until now this approach has not been applied to pathogens in avian species. To visualize the infection of an important avian pathogen, we generated Marek's disease virus (MDV) recombinants expressing firefly luciferase during lytic replication. Upon characterization of the recombinant viruses in vitro, chickens were infected and the infection visualized in live animals over the course of 14 days. The luminescence signal was consistent with the known spatiotemporal kinetics of infection and the life cycle of MDV, and correlated well with the viral load measured by qPCR. Intriguingly, this in vivo bioimaging approach revealed two novel sites of MDV replication, the beak and the skin of the feet covered in scales. Feet skin infection was confirmed using a complementary fluorescence bioimaging approach with MDV recombinants expressing mRFP or GFP. Infection was detected in the intermediate epidermal layers of the feet skin that was also shown to produce infectious virus, regardless of the animals' age at and the route of infection. Taken together, this study highlights the value of in vivo whole body bioimaging in avian species by identifying previously overlooked sites of replication and shedding of MDV in the chicken host.

Published
2022
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3. Four types of scrapie in goats differentiated from each other and bovine spongiform encephalopathy by biochemical methods

Author

Jan P. M. Langeveld, Laura Pirisinu, Jorg G. Jacobs, Maria Mazza, Isabelle Lantier, Stéphanie Simon, Olivier Andréoletti, Cristina Acin, Elena Esposito, Christine Fast, Martin Groschup, Wilfred Goldmann, John Spiropoulos, Theodoros Sklaviadis, Frederic Lantier, Loukia Ekateriniadou, Penelope Papasavva-Stylianou, Lucien J. M. van Keulen, Pier-Luigi Acutis, Umberto Agrimi, Alex Bossers, and Romolo Nonno

Subjects
Veterinary medicine, SF600-1100
Abstract

Abstract Scrapie in goats has been known since 1942, the archetype of prion diseases in which only prion protein (PrP) in misfolded state (PrPSc) acts as infectious agent with fatal consequence. Emergence of bovine spongiform encephalopathy (BSE) with its zoonotic behaviour and detection in goats enhanced fears that its source was located in small ruminants. However, in goats knowledge on prion strain typing is limited. A European-wide study is presented concerning the biochemical phenotypes of the protease resistant fraction of PrPSc (PrPres) in over thirty brain isolates from transmissible spongiform encephalopathy (TSE) affected goats collected in seven countries. Three different scrapie forms were found: classical scrapie (CS), Nor98/atypical scrapie and one case of CH1641 scrapie. In addition, CS was found in two variants—CS-1 and CS-2 (mainly Italy)—which differed in proteolytic resistance of the PrPres N-terminus. Suitable PrPres markers for discriminating CH1641 from BSE (C-type) appeared to be glycoprofile pattern, presence of two triplets instead of one, and structural (in)stability of its core amino acid region. None of the samples exhibited BSE like features. BSE and these four scrapie types, of which CS-2 is new, can be recognized in goats with combinations of a set of nine biochemical parameters.

Published
2019
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4. Effective Nanoparticle-Based Nasal Vaccine Against Latent and Congenital Toxoplasmosis in Sheep

Author

Céline Ducournau, Nathalie Moiré, Rodolphe Carpentier, Pauline Cantin, Clément Herkt, Isabelle Lantier, Didier Betbeder, and Isabelle Dimier-Poisson

Subjects
ovine toxoplasmosis, nanoparticle, nasal vaccination, adjuvant free, one-health approach, Immunologic diseases. Allergy, RC581-607
Abstract

Toxoplasma gondii is a parasitic protozoan of worldwide distribution, able to infect all warm-blooded animals, but particularly sheep. Primary infection in pregnant sheep leads to millions of abortions and significant economic losses for the livestock industry. Moreover, infected animals constitute the main parasitic reservoir for humans. Therefore, the development of a One-health vaccine seems the best prevention strategy. Following earlier work, a vaccine constituted of total extract of Toxoplasma gondii proteins (TE) associated with maltodextrin nanoparticles (DGNP) was developed in rodents. In this study we evaluated the ability of this vaccine candidate to protect against latent and congenital toxoplasmosis in sheep. After two immunizations by either intranasal or intradermal route, DGNP/TE vaccine generated specific Th1-cellular immune response, mediated by APC-secretion of IFN-γ and IL-12. Secretion of IL-10 appeared to regulate this Th1 response for intradermally vaccinated sheep but was absent in intranasally-vaccinated animals. Finally, protection against latent toxoplasmosis and transplacental transmission were explored. Intranasal vaccination led to a marked decrease of brain cysts compared with the non-vaccinated group. This DGNP/TE vaccine administered intranasally conferred a high level of protection against latent toxoplasmosis and its transplacental transmission in sheep, highlighting the potential for development of such a vaccine for studies in other species.

Published
2020
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5. Protein delivery by porous cationic maltodextrin-based nanoparticles into nasal mucosal cells: Comparison with cationic or anionic nanoparticles

Author

Minh Quan Lê, Rodolphe Carpentier, Isabelle Lantier, Céline Ducournau, François Fasquelle, Isabelle Dimier-Poisson, and Didier Betbeder

Subjects
Pharmacy and materia medica, RS1-441
Abstract

Different types of biodegradable nanoparticles (NPs) have been studied as delivery systems for proteins into nasal mucosal cells, especially for vaccine applications. Such a nanocarrier must have the ability to be loaded with proteins and to transport this payload into mucosal cells. However, comparative data on nanoparticles’ capacity for protein loading, efficiency of subsequent endocytosis and the quantity of nanocarriers used are either lacking or contradictory, making comparisons and the choice of a best candidate difficult. Here we compared 5 types of nanoparticles with different surface charge (anionic or cationic) and various inner compositions as potential vectors: the NPL (cationic maltodextrin NP with an anionic lipid core), cationic and anionic PLGA (Poly Lactic co-Glycolic Acid) NP, and cationic and anionic liposomes. We first quantified the protein association efficiency and NPL associated the largest amount of ovalbumin, used as a model protein. In vitro, the delivery of fluorescently-labeled ovalbumin into mucosal cells (airway epithelial cells, dendritic cells and macrophages) was assessed by flow cytometry and revealed that the NPL delivered protein to the greatest extent in all 3 different cell lines. Taken together, these data underlined the potential of the porous and cationic maltodextrin-based NPL as efficient protein delivery systems to mucosal cells. Keywords: Nanoparticles, Nasal, Vaccine, Protein delivery

Published
2019
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6. Systemic Administration of Avian Defensin 7: Distribution, Cellular Target, and Antibacterial Potential in Mice

Author

Geoffrey Bailleul, Rodrigo Guabiraba, Isabelle Virlogeux-Payant, Isabelle Lantier, Jérôme Trotereau, Florence B. Gilbert, Agnès Wiedemann, Angélina Trotereau, Philippe Velge, Catherine Schouler, and Anne-Christine Lalmanach

Subjects
chicken, defensins, mouse, macrophages, Salmonella Typhimurium, Microbiology, QR1-502
Abstract

Defensins are natural antimicrobial peptides. The avian beta-defensin AvBD7 isolated from the chicken bone marrow possess broad antibacterial spectrum and strong resistance to proteolysis. However, its ability to fight systemic infections of major concern for public health, such as salmonellosis, is unknown. As a first approach, fluorescence labeling of AvBD7 allowed to track its systemic distribution after intraperitoneal injection in mice using whole body live imaging. It was associated to peritoneal cells and to deeper organs such as the liver. In the next step, the use of labeled AvBD7 allowed to observe its interaction with murine macrophages in culture. After incubation, it was able to penetrate inside the cells through an endocytosis-like mechanism. Furthermore, natural AvBD7 contributed to the control of intracellular multiplication of a multidrug resistant Salmonella strain, after incubation with infected macrophages. Finally, administration in a model of systemic lethal Salmonella infection in mice led to significant improvement of mouse survival, consistently with significant reduction of the liver bacterial load. In conclusion, the results reveal a hitherto unknown intracellular antibacterial effect of AvBD7 in Salmonella target cells and support AvBD7 as a candidate of interest for the treatment of infectious diseases caused by multidrug-resistant pathogenic Enterobacteriaceae.

Published
2019
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7. Transgenic Mice Expressing Porcine Prion Protein Resistant to Classical Scrapie but Susceptible to Sheep Bovine Spongiform Encephalopathy and Atypical Scrapie

Author

Juan-Carlos Espinosa, María-Eugenia Herva, Olivier Andréoletti, Danielle Padilla, Caroline Lacroux, Hervé Cassard, Isabelle Lantier, Joaquin Castilla, and Juan-María Torres

Subjects
Porcine prion, bovine spongiform encephalopathy, sheep, scrapie, transmission barrier, prions and related diseases, Medicine, Infectious and parasitic diseases, RC109-216
Abstract

How susceptible pigs are to infection with sheep prions is unknown. We show, through transmission experiments in transgenic mice expressing porcine prion protein (PrP), that the susceptibility of this mouse model to bovine spongiform encephalopathy (BSE) can be enhanced after its passage in ARQ sheep, indicating that the pathogenicity of the BSE agent is modified after passage in sheep. Transgenic mice expressing porcine PrP were, nevertheless, completely resistant to infection with a broad panel of classical scrapie isolates from different sheep PrP genotypes and with different biochemical characteristics. The atypical (Nor98 like) isolate (SC-PS152) was the only scrapie isolate capable of transmission in these mice, although with a marked transmission barrier. Unexpectedly, the atypical scrapie agent appeared to undergo a strain phenotype shift upon transmission to porcine-PrP transgenic mice and acquired new strain properties, suggesting that atypical scrapie agent may exhibit different phenotypes depending on the host cellular PrP or other genetic factors.

Published
2009
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8. Preclinical detection of variant CJD and BSE prions in blood.

Author

Caroline Lacroux, Emmanuel Comoy, Mohammed Moudjou, Armand Perret-Liaudet, Séverine Lugan, Claire Litaise, Hugh Simmons, Christelle Jas-Duval, Isabelle Lantier, Vincent Béringue, Martin Groschup, Guillaume Fichet, Pierrette Costes, Nathalie Streichenberger, Frederic Lantier, Jean Philippe Deslys, Didier Vilette, and Olivier Andréoletti

Subjects
Immunologic diseases. Allergy, RC581-607, Biology (General), QH301-705.5
Abstract

The emergence of variant Creutzfeldt Jakob Disease (vCJD) is considered a likely consequence of human dietary exposure to Bovine Spongiform Encephalopathy (BSE) agent. More recently, secondary vCJD cases were identified in patients transfused with blood products prepared from apparently healthy donors who later went on to develop the disease. As there is no validated assay for detection of vCJD/BSE infected individuals the prevalence of the disease in the population remains uncertain. In that context, the risk of vCJD blood borne transmission is considered as a serious concern by health authorities. In this study, appropriate conditions and substrates for highly efficient and specific in vitro amplification of vCJD/BSE agent using Protein Misfolding Cyclic Amplification (PMCA) were first identified. This showed that whatever the origin (species) of the vCJD/BSE agent, the ovine Q171 PrP substrates provided the best amplification performances. These results indicate that the homology of PrP amino-acid sequence between the seed and the substrate is not the crucial determinant of the vCJD agent propagation in vitro. The ability of this method to detect endogenous vCJD/BSE agent in the blood was then defined. In both sheep and primate models of the disease, the assay enabled the identification of infected individuals in the early preclinical stage of the incubation period. Finally, sample panels that included buffy coat from vCJD affected patients and healthy controls were tested blind. The assay identified three out of the four tested vCJD affected patients and no false positive was observed in 141 healthy controls. The negative results observed in one of the tested vCJD cases concurs with results reported by others using a different vCJD agent blood detection assay and raises the question of the potential absence of prionemia in certain patients.

Published
2014
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9. Copy number variation and differential expression of a protective endogenous retrovirus in sheep.

Author

Barbara Viginier, Christine Dolmazon, Isabelle Lantier, Frédéric Lantier, Fabienne Archer, Caroline Leroux, and Christophe Terzian

Subjects
Medicine, Science
Abstract

The Jaagsiekte sheep retrovirus exJSRV and its endogenous counterpart enJSRV co-exist in sheep. exJSRV, a betaretrovirus, is the etiological agent of ovine pulmonary adenocarcinoma, and it has been demonstrated in vitro that an enJSRV Gag variant bearing the R-to-W amino acid change at position 21 was able to block exJSRV budding from the cells, providing a potential protective role for the host. In this work, we developed a fast mutation detection assay based on the oligo ligation assay (OLA) that permits the quantification of the relative proportions of the R21 and W21 Gag variants present in individual genomes and in cDNA obtained from normal and exJSRV-induced lung tumors. We have shown that the W21/R21 ratio is variable within and between breeds. We also describe for the first time that putative protecting enJSRV variants were expressed in alveolar type II cells (AECII), the major target of exJSRV.

Published
2012
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10. Topical reinforcement of the cervical mucus barrier to sperm

Author

Ulrike Schimpf, Erika Caldas-Silveira, Ljudmila Katchan, Cécile Vigier-Carriere, Isabelle Lantier, Gilai Nachmann, Sebastian Gidlöf, Aino Fianu Jonasson, Lars Björndahl, Stéphane Trombotto, Xavier Druart, and Thomas Crouzier

Subjects
Male, Chitosan, Sheep, Contraceptive Agents, Pregnancy, Semen, Cervix Mucus, Sperm Motility, Humans, Animals, Female, General Medicine, Spermatozoa
Abstract

Close to half of the world's pregnancies are still unplanned, reflecting a clear unmet need in contraception. Ideally, a contraceptive would provide the high efficacy of hormonal treatments, without the systemic side effects of hormones. Here we propose topical reinforcement of the cervical mucus by chitosan mucoadhesive polymers as a form of female contraceptive. Chitosans larger than 7 kDa effectively crosslinked human ovulatory cervical mucus to prevent sperm penetration in vitro. We then demonstrated in vivo using the ewe as a model that vaginal gels containing chitosan could stop ram sperm at the entrance of the cervical canal and prevent them from reaching the uterus, whereas the same gels without chitosan did not significantly limit sperm migration. The chitosan did not affect sperm motility in vitro or in vivo, suggesting reinforcement of the mucus physical barrier as the primary mechanism of action. The chitosan formulations did not damage or irritate the ewe vaginal epithelium, in contrast to nonoxynol-9 spermicide. The demonstration that cervical mucus can be reinforced topically to create an effective barrier to sperm may therefore form the technological basis for muco-cervical barrier contraceptives with the potential to become an alternative to hormonal contraceptives., Find the editorial version at the aaa.org website: https://www.science.org/doi/10.1126/scitranslmed.abm2417

Published
2022

11. In Vivo Tracking of Bacterial Colonization in Different Murine Models Using Bioluminescence: The Example of Salmonella

Author

Michaël, Koczerka, Isabelle, Lantier, Anne, Pinard, Marie, Morillon, Justine, Deperne, Ohad, Gal-Mor, Olivier, Grépinet, and Isabelle, Virlogeux-Payant

Subjects
Salmonella typhimurium, Disease Models, Animal, Mice, Guinea Pigs, Salmonella Infections, Ferrets, Animals, Chickens
Abstract

Applications of bioluminescence for the in vivo study of pathogenic microorganisms are numerous, ranging from the quantification of virulence gene expression to measuring the effect of antimicrobial molecules on the colonization of tissues and organs by the pathogen. Most studies are performed in mice, but recent works demonstrate that this technique is applicable to larger animals like fish, guinea pigs, ferrets, and chickens. Here, we describe the construction and the utilization of a constitutively luminescent strain of Salmonella Typhimurium to monitor in vivo and ex vivo the colonization of mice in the gastroenteritis, typhoid fever, and asymptomatic carriage models of Salmonella infection.

Published
2022

12. Evidence of early increased sialylation of airway mucins and defective mucociliary clearance in CFTR-deficient piglets

Author

Catherine Robbe-Masselot, Mustapha Si-Tahar, Ignacio S. Caballero, Renaud Léonard, Nicolas Pons, Andrea Bähr, Pascal Barbry, Antoine Guillon, Agnès Paquet, Claire Chevaleyre, Nikolai Klymiuk, Isabelle Fleurot, Mustapha Berri, Bélinda Ringot-Destrez, Kevin Lebrigand, Carole Baron, Céline Barc, Reuben Ramphal, Isabelle Lantier, Centre National de la Recherche Scientifique (CNRS), Université Côte d'Azur (UCA), Institut de pharmacologie moléculaire et cellulaire (IPMC), Centre National de la Recherche Scientifique (CNRS)-Université Nice Sophia Antipolis (... - 2019) (UNS), COMUE Université Côte d'Azur (2015 - 2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015 - 2019) (COMUE UCA)-Université Côte d'Azur (UCA), Infectiologie et Santé Publique (UMR ISP), Université de Tours (UT)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Unité de Glycobiologie Structurale et Fonctionnelle - UMR 8576 (UGSF), Université de Lille-Centre National de la Recherche Scientifique (CNRS), Centre d’Etude des Pathologies Respiratoires (CEPR), UMR 1100 (CEPR), Université de Tours (UT)-Institut National de la Santé et de la Recherche Médicale (INSERM), Université Nice Sophia Antipolis (1965 - 2019) (UNS), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Centre National de la Recherche Scientifique (CNRS)-Université Côte d'Azur (UCA), Service de Médecine Intensive et Réanimation [Tours], Plateforme d'Infectiologie Expérimentale (PFIE), Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Ludwig Maximilian University [Munich] (LMU), Association Vaincre la Mucoviscidose (Grant No. RF20150 501357), Association Grégory Lemarchal (Grant No. RIF20160501690), Fondation pour la Recherche Médicale (DEQ20180339158), ANR-11-LABX-0028,SIGNALIFE,Réseau d'Innovation sur les Voies de Signalisation en Sciences de la Vie(2011), ANR-10-INBS-0009,France-Génomique,Organisation et montée en puissance d'une Infrastructure Nationale de Génomique(2010), Chanteloup, Nathalie Katy, Centres d'excellences - Réseau d'Innovation sur les Voies de Signalisation en Sciences de la Vie - - SIGNALIFE2011 - ANR-11-LABX-0028 - LABX - VALID, Organisation et montée en puissance d'une Infrastructure Nationale de Génomique - - France-Génomique2010 - ANR-10-INBS-0009 - INBS - VALID, Université de Tours (UT), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Université Côte d'Azur (UCA), Centre Hospitalier Régional Universitaire de Tours (CHRU Tours), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Tours (UT), Unité de Glycobiologie Structurale et Fonctionnelle (UGSF), Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Gene Center and Center for Innovation Medical Models (CiMM), Ludwig Maximilians University of Munich, Université de Tours-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Université de Tours-Institut National de la Santé et de la Recherche Médicale (INSERM), and Centre Hospitalier Régional Universitaire de Tours (CHRU TOURS)

Subjects
0301 basic medicine, Pulmonary and Respiratory Medicine, Male, Glycosylation, Mucociliary clearance, Swine, [SDV]Life Sciences [q-bio], Sus scrofa, Cystic Fibrosis Transmembrane Conductance Regulator, Context (language use), Inflammation, Respiratory Mucosa, Mucociliary transport, medicine.disease_cause, Cystic fibrosis, Microbiology, 03 medical and health sciences, 0302 clinical medicine, Medicine, Animals, CFTR, ComputingMilieux_MISCELLANEOUS, Lung, medicine.diagnostic_test, business.industry, Pseudomonas aeruginosa, Mucin, Mucins, respiratory system, medicine.disease, 3. Good health, respiratory tract diseases, Trachea, [SDV] Life Sciences [q-bio], Mucin glycosylation, 030104 developmental biology, medicine.anatomical_structure, Bronchoalveolar lavage, 030228 respiratory system, Animals, Newborn, Mucociliary Clearance, Pediatrics, Perinatology and Child Health, Female, medicine.symptom, MESH: CFTR, business
Abstract

International audience; Background: Bacterial colonization in cystic fibrosis (CF) lungs has been directly associated to the loss of CFTR function, and/or secondarily linked to repetitive cycles of chronic inflammation/infection. We hypothesized that altered molecular properties of mucins could contribute to this process.Methods: Newborn CFTR+/+ and CFTR-/- were sacrificed before and 6 h after inoculation with luminescent Pseudomonas aeruginosa into the tracheal carina. Tracheal mucosa and the bronchoalveolar lavage (BAL) fluid were collected to determine the level of mucin O-glycosylation, bacteria binding to mucins and the airways transcriptome. Disturbances in mucociliary transport were determined by ex-vivo imaging of luminescent Pseudomonas aeruginosa.Results: We provide evidence of an increased sialylation of CF airway mucins and impaired mucociliary transport that occur before the onset of inflammation. Hypersialylation of mucins was reproduced on tracheal explants from non CF animals treated with GlyH101, an inhibitor of CFTR channel activity, indicating a causal relationship between the absence of CFTR expression and the sialylation of mucins. This increased sialylation was correlated to an increased adherence of P. aeruginosa to mucins. In vivo infection of newborn CF piglets by live luminescent P. aeruginosa demonstrated an impairment of mucociliary transport of this bacterium, with no evidence of pre-existing inflammation.Conclusions: Our results document for the first time in a well-defined CF animal model modifications that affect the O-glycan chains of mucins. These alterations precede infection and inflammation of airway tissues, and provide a favorable context for microbial development in CF lung that hallmarks this disease.

Published
2020
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13. Characterization of goat prions demonstrates geographical variation of scrapie strains in Europe and reveals the composite nature of prion strains

Author

Lucien van Keulen, Patricia Aguilar-Calvo, John Spiropoulos, Claudia D'Agostino, Christine Fast, Pier Luigi Acutis, Penelope Papasavva-Stylianou, Alex Bossers, Romolo Nonno, Frédéric Lantier, Olivier Andreoletti, Gabriele Vaccari, Isabelle Lantier, Jorge G Jacobs, Juan María Torres, Jan P. M. Langeveld, Martin H. Groschup, Umberto Agrimi, Juan Carlos Espinosa, Cristina Acín, Barbara Chiappini, Theodoros Sklaviadis, Michele Angelo Di Bari, Laura Pirisinu, Alba Marín-Moreno, Istituto Superiore di Sanità (ISS), Centro de Investigacion en Sanidad Animal (INIA-CISA), Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria = National Institute for Agricultural and Food Research and Technology (INIA), Institute of Novel and Emerging Infectious Diseases (INNT), Friedrich-Loeffler-Institut (FLI), Wageningen BioVeterinary Research, Wageningen University and Research [Wageningen] (WUR), Animal and Plant Health Agency [Addlestone, UK] (APHA), Infectiologie et Santé Publique (UMR ISP), Université de Tours (UT)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Interactions hôtes-agents pathogènes [Toulouse] (IHAP), Ecole Nationale Vétérinaire de Toulouse (ENVT), Institut National Polytechnique (Toulouse) (Toulouse INP), Université de Toulouse (UT)-Université de Toulouse (UT)-Institut National Polytechnique (Toulouse) (Toulouse INP), Université de Toulouse (UT)-Université de Toulouse (UT)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Aristotle University of Thessaloniki, Veterinary Services of Cyprus, Istituto Zooprofilattico Sperimentale del Piemonte, Liguria e Valle d'Aosta, University of Zaragoza - Universidad de Zaragoza [Zaragoza], Centro de Encefalopatías y Enfermedades Transmisibles Emergentes, This work was funded by the European Union (FOOD-CT-2006-36353 to AB, JPML, OA, JMT, CA, MHG, PLA, UA, TS, and ERA-NET EMIDA GOAT-TSE-FREE to JPML, LVK, PLA, OA, JCE, CA, CF), the Dutch Ministry of Agriculture (project WOT-01-002-001.01 to JPML), the Spanish Ministerio de ciencia innovacion y universidades (AGL201678054-R [AEI/FEDER, UE] and AGL2012-37988-C04-04 to JMT), the Italian Ministry of Health (RF-20091474624 to RN). AMM was supported by a fellowship from the INIA (FPI-SGIT-2015-02). PAC was supported by a fellowship from the Spanish Ministerio de Economia y Competitividad (BES-2010-040922)., European Project: 36353,GOATBSE, Istituto Superiore di Sanita [Rome], Université de Tours-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National Polytechnique (Toulouse) (Toulouse INP), and Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)

Subjects
0301 basic medicine, Prion diseases, Prions, [SDV]Life Sciences [q-bio], animal diseases, Bovine spongiform encephalopathy, lcsh:Medicine, Prion strain, Scrapie, Biology, Prion Proteins, Article, Mice, 03 medical and health sciences, 0302 clinical medicine, Animal model, Biological property, medicine, Animals, Life Science, Neurodegeneration, lcsh:Science, Host Pathogen Interaction & Diagnostics, Genetics, Goat Diseases, [SDV.BA.MVSA]Life Sciences [q-bio]/Animal biology/Veterinary medicine and animal Health, Multidisciplinary, Goats, Strain (biology), lcsh:R, Bacteriologie, Bacteriology, Bacteriology, Host Pathogen Interaction & Diagnostics, Limiting, medicine.disease, Host Pathogen Interactie & Diagnostiek, nervous system diseases, Encephalopathy, Bovine Spongiform, Europe, Experimental models of disease, 030104 developmental biology, Bacteriologie, Host Pathogen Interactie & Diagnostiek, Infectious diseases, Cattle, lcsh:Q, 030217 neurology & neurosurgery
Abstract

Bovine Spongiform Encephalopathy (BSE) is the only animal prion which has been recognized as a zoonotic agent so far. The identification of BSE in two goats raised the need to reliably identify BSE in small ruminants. However, our understanding of scrapie strain diversity in small ruminants remains ill-defined, thus limiting the accuracy of BSE surveillance and spreading fear that BSE might lurk unrecognized in goats. We investigated prion strain diversity in a large panel of European goats by a novel experimental approach that, instead of assessing the neuropathological profile after serial transmissions in a single animal model, was based on the direct interaction of prion isolates with several recipient rodent models expressing small ruminants or heterologous prion proteins. The findings show that the biological properties of scrapie isolates display different patterns of geographical distribution in Europe and suggest that goat BSE could be reliably discriminated from a wide range of biologically and geographically diverse goat prion isolates. Finally, most field prion isolates showed composite strain features, with discrete strain components or sub-strains being present in different proportions in individual goats or tissues. This has important implications for understanding the nature and evolution of scrapie strains and their transmissibility to other species, including humans.

Published
2020
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14. Four types of scrapie in goats differentiated from each other and bovine spongiform encephalopathy by biochemical methods

Author

Cristina Acín, John Spiropoulos, Laura Pirisinu, J. G. Jacobs, Christine Fast, Jan P. M. Langeveld, Romolo Nonno, Frédéric Lantier, Olivier Andreoletti, Loukia V. Ekateriniadou, Umberto Agrimi, Elena Esposito, Stéphanie Simon, Maria Mazza, Alex Bossers, Martin H. Groschup, Isabelle Lantier, Theodoros Sklaviadis, Wilfred Goldmann, Pier Luigi Acutis, Penelope Papasavva-Stylianou, Lucien van Keulen, Wageningen BioVeterinary Research, Wageningen University and Research [Wageningen] (WUR), Istituto Superiore di Sanita [Rome], Istituto Zooprofilattico Sperimentale del Piemonte, IZSPLVA, Italian Reference Centre for Animal TSE, Infectiologie et Santé Publique (UMR ISP), Institut National de la Recherche Agronomique (INRA)-Université de Tours (UT), Commissariat à l’Energie Atomique, Gif-sur-Yvette, France, Interactions hôtes-agents pathogènes [Toulouse] (IHAP), Institut National de la Recherche Agronomique (INRA)-Ecole Nationale Vétérinaire de Toulouse (ENVT), Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées, University of Zaragoza - Universidad de Zaragoza [Zaragoza], Institute of Novel and Emerging Infectious Diseases (INNT), Friedrich-Loeffler-Institut (FLI), University of Edinburgh, Royal Dick Sch Vet Studies, Roslin BioCtr, Roslin EH25 9PS, Animal and Plant Health Agency [Addlestone, UK] (APHA), Aristotle University of Thessaloniki, Hellenic Agricultural Organization Demeter (HAO Demeter), Veterinary Services of Cyprus, Ministry of Agriculture Rural Development and Environment, Partenaires INRAE, This work was supported by the European Commission: NeuroPrion [EC FOOD‑CT‑2004‑506579], GoatBSE [EC FOOD‑CT‑2006‑36353], GOAT‑TSE‑FREE [ERA‑NET EMIDA] and by National fundings: Netherlands—Ministry of Agriculture, Nature and Food Quality [WOT 01‑002‑001.01], Italy—Italian Ministry of Health [RF‑2009‑1474624], Spain—Ministry of Economy and Competitiveness [AGL2012‑40071], United Kingdom—Department for Environment, Food and Rural Affairs [SE2018] [SE1961], Biotechnology and Biological Sciences Research Council [BB/J004332/1], Greece—Greek Ministry of Education, and France—Institut National de la Recherche Agronomique TRANSPRION [EFA282/13], REDPRION [EFA148/16], European Project, European Project: 26570,NEUROPRION, European Project: 39519,GOATBSE, European Project: 36353,GOATBSE, European Project: 219235,EC:FP7:KBBE,FP7-ERANET-2007-RTD,EMIDA(2008), Langeveld, Jan P M, Istituto Superiore di Sanità (ISS), Université de Toulouse (UT)-Université de Toulouse (UT)-Institut National Polytechnique (Toulouse) (Toulouse INP), Université de Toulouse (UT)-Université de Toulouse (UT), and Institut National de la Recherche Agronomique (INRA)-Université de Tours

Subjects
0301 basic medicine, 040301 veterinary sciences, Bovine spongiform encephalopathy, [SDV]Life Sciences [q-bio], animal diseases, Blotting, Western, Scrapie, Enzyme-Linked Immunosorbent Assay, polymorphisme, Biology, 0403 veterinary science, prion, 03 medical and health sciences, Protease resistant, medicine, Life Science, biochimie des protéines, Animals, Typing, Prion protein, Host Pathogen Interaction & Diagnostics, Transmissible spongiform encephalopathy, lcsh:Veterinary medicine, Goat Diseases, General Veterinary, tremblante, Goats, Bacteriologie, Bacteriology, 04 agricultural and veterinary sciences, Bacteriology, Host Pathogen Interaction & Diagnostics, Classical scrapie, medicine.disease, Virology, Host Pathogen Interactie & Diagnostiek, encéphalopathie spongiforme bovine, nervous system diseases, Europe, 030104 developmental biology, Bacteriologie, Host Pathogen Interactie & Diagnostiek, lcsh:SF600-1100, Infectious agent, Research Article
Abstract

Scrapie in goats has been known since 1942, the archetype of prion diseases in which only prion protein (PrP) in misfolded state (PrPSc) acts as infectious agent with fatal consequence. Emergence of bovine spongiform encephalopathy (BSE) with its zoonotic behaviour and detection in goats enhanced fears that its source was located in small ruminants. However, in goats knowledge on prion strain typing is limited. A European-wide study is presented concerning the biochemical phenotypes of the protease resistant fraction of PrPSc (PrPres) in over thirty brain isolates from transmissible spongiform encephalopathy (TSE) affected goats collected in seven countries. Three different scrapie forms were found: classical scrapie (CS), Nor98/atypical scrapie and one case of CH1641 scrapie. In addition, CS was found in two variants—CS-1 and CS-2 (mainly Italy)—which differed in proteolytic resistance of the PrPresN-terminus. Suitable PrPres markers for discriminating CH1641 from BSE (C-type) appeared to be glycoprofile pattern, presence of two triplets instead of one, and structural (in)stability of its core amino acid region. None of the samples exhibited BSE like features. BSE and these four scrapie types, of which CS-2 is new, can be recognized in goats with combinations of a set of nine biochemical parameters.

Published
2019
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15. Sélection génétique pour la résistance aux maladies : projets et avancées

Author

Mélanie Gunia, Merina Shrestha, Elodie Balmisse, Bertrand Bed'Hom, Stéphane Bertagnoli, Samuel Boucher, Sylvain Breton, Emilie Chambellon, Thierry Chaumeil, Fabien Coisne, Ingrid David, Jean-Jacques David, Edouard Guitton, Virginie Helies, Jacques Hurtaud, Florent Kempf, Isabelle Lantier, Sébastien Lavillate, Dominique Le Creu, Guillaume Lenoir, Bernadette Le Normand, Coralie Marais, Mickaël Maupin, Hervé Morin, Charles Poncet, Sébastien Pujol, Raphaël Robert, Christelle Rossignol, Julien Ruesche, Fanny Sarce, Colomba Thiebot, Emmanuelle Helloin, Frederic Lantier, Hervé Garreau, Génétique Physiologie et Systèmes d'Elevage (GenPhySE ), École nationale supérieure agronomique de Toulouse [ENSAT]-Institut National de la Recherche Agronomique (INRA)-Ecole Nationale Vétérinaire de Toulouse (ENVT), Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées, Pôle d'Expérimentation Cunicole TOULousain (PECTOUL ), Institut National de la Recherche Agronomique (INRA), Génétique Animale et Biologie Intégrative (GABI), Institut National de la Recherche Agronomique (INRA)-AgroParisTech, Interactions hôtes-agents pathogènes [Toulouse] (IHAP), Institut National de la Recherche Agronomique (INRA)-Ecole Nationale Vétérinaire de Toulouse (ENVT), Labovet Conseil, Plateforme d'Infectiologie Expérimentale (PFIE), Infectiologie et Santé Publique (UMR ISP), Institut National de la Recherche Agronomique (INRA)-Université de Tours (UT), Hycole Sarl, Hypharm, Clinical Innovation Proteomic Platform, Partenaires INRAE, VeLVet, Institut de Recherche en Horticulture et Semences (IRHS), AGROCAMPUS OUEST, Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut National de la Recherche Agronomique (INRA)-Université d'Angers (UA), Filavie SAS, Génétique Diversité et Ecophysiologie des Céréales (GDEC), Institut National de la Recherche Agronomique (INRA)-Université Clermont Auvergne [2017-2020] (UCA [2017-2020]), Eurolap, Institut Carnot, AgroParisTech-Institut National de la Recherche Agronomique (INRA), Institut National de la Recherche Agronomique (INRA)-Université de Tours, Université d'Angers (UA)-Institut National de la Recherche Agronomique (INRA)-AGROCAMPUS OUEST, Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), Université de Toulouse (UT)-Université de Toulouse (UT)-Institut National Polytechnique (Toulouse) (Toulouse INP), Université de Toulouse (UT)-Université de Toulouse (UT)-École nationale supérieure agronomique de Toulouse (ENSAT), and Université de Toulouse (UT)-Université de Toulouse (UT)

Subjects
sélection, génétique, résistance au maladie, bien être animal, [SDV.GEN]Life Sciences [q-bio]/Genetics, [SDV.GEN.GA]Life Sciences [q-bio]/Genetics/Animal genetics, [SDV]Life Sciences [q-bio], [SDE]Environmental Sciences, [SDV.BV]Life Sciences [q-bio]/Vegetal Biology, [INFO]Computer Science [cs], ComputingMilieux_MISCELLANEOUS
Abstract

National audience

Published
2018

16. Structural and functional diversity arising from intra- and inter-haplotype combinations of duplicated DQA and B loci within the ovine MHC

Author

Frédéric Lantier, Keith T. Ballingall, Isabelle Lantier, Helen Todd, Mara Rocchi, Pentlands Science Park, Moredun Research Institute [Penicuik, UK] (MRI), Infectiologie et Santé Publique (UMR ISP), Institut National de la Recherche Agronomique (INRA)-Université de Tours (UT), European Project: 228394,EC:FP7:INFRA,FP7-INFRASTRUCTURES-2008-1,NADIR(2009), and Institut National de la Recherche Agronomique (INRA)-Université de Tours

Subjects
0301 basic medicine, Models, Molecular, Genotype, Protein Conformation, Immunology, Locus (genetics), Expression, Biology, Major histocompatibility complex, 03 medical and health sciences, Functional diversity, 0302 clinical medicine, Gene Frequency, Sequence Homology, Nucleic Acid, Gene duplication, Genetics, Haplotype, Animals, Amino Acid Sequence, Allele, MHC class II DQ, Gene, Alleles, Phylogeny, Sheep, Base Sequence, Sequence Homology, Amino Acid, [SDV.BA]Life Sciences [q-bio]/Animal biology, Histocompatibility Antigens Class II, Genetic Variation, Human genetics, Ovine, 030104 developmental biology, Haplotypes, biology.protein, [SDV.IMM]Life Sciences [q-bio]/Immunology, 030215 immunology
Abstract

Electronic supplementary material The online version of this article (https://doi.org/10.1007/s00251-017-1029-z) contains supplementarymaterial, which is available to authorized users; International audience; In sheep, the A and B loci encoding the α and β chains of the classical class II MHC molecules are DRA and DRB and DQA and DQB. Previous analyses described the duplication of the DQA and DQB genes. The majority of haplotypes include DQA1 and DQA2 loci, however, in a number of haplotypes, DQA1 appears absent and these haplotypes have been described as DQA1 null. In these haplotypes, the DQA2 locus is found in combination with a second locus which appeared more closely related to DQA2 than DQA1, hence the description of this locus as DQA2-like. Here we combine our previous analysis of the DQA transcripts with an analysis of the associated DQB transcripts in ten haplotypes from MHC homozygous animals. This allows the potential for surface expression of different haplotype combinations of DQA and B genes and the functional significance of DQA2-like and its predicted DQB partner to be determined. Atypical DQB transcripts (DQB2-like) were identified in haplotypes classified as DQA1-null and conserved DQB2-like orthologues were identified in other Bovidae indicating trans-species conservation of the allelic lineage. Functional combinations detected by co-transfection of DQ1, DQ2 and DQ2-like genes demonstrates the potential for a wide range of DQ molecules derived from both intra- and inter-haplotype as well as inter-locus combinations. We provide evidence that DQA2-like and B2-like genes form an evolutionary conserved pair which generates structurally distinct class II molecules that are likely to present a distinct range of peptides to CD4+ T cells.

Published
2018
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17. Residence time and uptake of porous and cationic maltodextrin-based nanoparticles in the nasal mucosa: comparison with anionic and cationic nanoparticles

Author

Rodolphe Carpentier, Didier Betbeder, Minh Quan Le, Céline Ducournau, Isabelle Lantier, Isabelle Dimier-Poisson, Lille Inflammation Research International Center - U 995 (LIRIC), Institut Pasteur de Lille, Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Lille-Centre Hospitalier Régional Universitaire [Lille] (CHRU Lille), Infectiologie et Santé Publique (UMR ISP), Institut National de la Recherche Agronomique (INRA)-Université de Tours (UT), Université d'Artois (UA), European Project: 607690,EC:FP7:PEOPLE,FP7-PEOPLE-2013-ITN,UNIVACFLU(2013), Centre Hospitalier Régional Universitaire [Lille] (CHRU Lille)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Lille-Institut Pasteur de Lille, Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP), and Institut National de la Recherche Agronomique (INRA)-Université de Tours

Subjects
0301 basic medicine, [SDV.OT]Life Sciences [q-bio]/Other [q-bio.OT], Respiratory System, Pharmaceutical Science, Nanoparticle, 02 engineering and technology, Endocytosis, Cell Line, Mucosa, 03 medical and health sciences, chemistry.chemical_compound, Mice, Polylactic Acid-Polyglycolic Acid Copolymer, Live cell imaging, Polysaccharides, Animals, Humans, Administration, Intranasal, Liposome, Nasal, Cationic polymerization, 021001 nanoscience & nanotechnology, Maltodextrin, PLGA, Nasal Mucosa, 030104 developmental biology, chemistry, Liposomes, Biophysics, Nanoparticles, Nasal administration, 0210 nano-technology, Porosity, Vaccine
Abstract

International audience; Different types of biodegradable nanoparticles (NP) have been studied as nasal mucosa cell delivery systems. These nanoparticles need to strongly interact with mucosa cells to deliver their payload. However, only a few simultaneous comparisons have been made and it is therefore difficult to determine the best candidate. Here we compared 5 types of nanoparticles with different surface charge (anionic or cationic) and various inner compositions as potential vectors: cationic and anionic liposomes, cationic and anionic PLGA (Poly Lactic co-Glycolic Acid) NP and porous and cationic maltodextrin NP (cationic surface with an anionic lipid core: NPL). We first quantified their nasal residence time after nasal administration in mice using in vivo live imaging and NPL showed the longest residence time. In vitro endocytosis on mucosal cells (airway epithelial cells, macrophages and dendritic cells) using labeled nanoparticles were performed by flow cytometry and confocal microscopy. Among the 5 nanoparticles, NPL were taken up to the greatest extent by the 3 different cell lines and the endocytosis mechanisms were characterized. Taken together, we observed that the nanoparticles' cationic surface charge is insufficient to improve mucosal residence time and cellular uptake and that the NPL are the best candidates to interact with airway mucosal cells.

Published
2018
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18. Premiers résultats du projet RELAPA : génomique pour la résistance génétique des lapins à la pasteurellose

Author

Mélanie Gunia, Frederic Lantier, Jean-Marc Babilliot, Elodie Balmisse, Bertrand Bed'Hom, Elodie Belmonte, Stéphane Bertagnoli, Samuel Boucher, Sylvain Breton, Emilie Chambellon, Thierry Chaumeil, Fabien Coisne, Rémi Delaunay, Alain Fadeau, Edouard Guitton, Virginie Helies, Jacques Hurtaud, Deborah Jardet, Florent Kempf, Isabelle Lantier, Sébastien Lavillatte, Dominique Le Cren, Guillaume Lenoir, Bernadette Le Normand, Coralie Marais, Mickaël Maupin, Hervé Morin, Charles Poncet, Sébastien Pujol, Raphaël Robert, Christelle Rossignol, Julien Ruesche, Fanny Sarce, Colomba Thiebot, Emmanuelle Helloin, Herve Garreau, Génétique Physiologie et Systèmes d'Elevage (GenPhySE ), École nationale supérieure agronomique de Toulouse [ENSAT]-Institut National de la Recherche Agronomique (INRA)-Ecole Nationale Vétérinaire de Toulouse (ENVT), Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées, Infectiologie et Santé Publique (UMR ISP), Institut National de la Recherche Agronomique (INRA)-Université de Tours (UT), Génétique Animale et Biologie Intégrative (GABI), Institut National de la Recherche Agronomique (INRA)-AgroParisTech, Pôle d'Expérimentation Cunicole TOULousain (PECTOUL ), Institut National de la Recherche Agronomique (INRA), Génétique Diversité et Ecophysiologie des Céréales (GDEC), Institut National de la Recherche Agronomique (INRA)-Université Clermont Auvergne [2017-2020] (UCA [2017-2020]), Interactions hôtes-agents pathogènes [Toulouse] (IHAP), Institut National de la Recherche Agronomique (INRA)-Ecole Nationale Vétérinaire de Toulouse (ENVT), LABOVET Conseil, Réseau Cristal, Plateforme d'Infectiologie Expérimentale (PFIE), Hycole Sarl, Laboratoire de Touraine, Hypharm, Comité Lapin Interprofessionnel pour la Promotion des Produits Français (CLIPP), VeLVet, Filavie SAS, Eurolap, Institut Technique de l'Aviculture et des Elevages de Petits Animaux (ITAVI). FRA., Université de Toulouse (UT)-Université de Toulouse (UT)-Institut National Polytechnique (Toulouse) (Toulouse INP), Université de Toulouse (UT)-Université de Toulouse (UT)-École nationale supérieure agronomique de Toulouse (ENSAT), Université de Toulouse (UT)-Université de Toulouse (UT), Institut National de la Recherche Agronomique (INRA)-Université de Tours, AgroParisTech-Institut National de la Recherche Agronomique (INRA), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-École nationale supérieure agronomique de Toulouse [ENSAT], Infectiologie Animale et Santé Publique - IASP (Nouzilly, France), UE 1322 Pôle d'Expérimentation Cunicole TOULousain, Institut National de la Recherche Agronomique (INRA)-Génétique animale (G.A.)-Physiologie Animale et Systèmes d'Elevage (PHASE), Institut National de la Recherche Agronomique (INRA)-Pôle d'Expérimentation Cunicole TOULousain (PECTOUL), Institut National de la Recherche Agronomique (INRA)-Université Blaise Pascal - Clermont-Ferrand 2 (UBP), Plateforme d'Infectiologie Expérimentale (PFIE - INRA UE 1277), and HYCOLE

Subjects
pasteurella multocida, pasteurellose, [SDV]Life Sciences [q-bio], genetic variability, variabilité génétique, rabbit, résistance, lapin, résistance génétique, génétique, génomique
Abstract

Session : Génétique Participation à cette étude : Plateforme d'Infectiologie Expérimentale (PFIE) UE 1277 - Nouzilly Centre Inra Val de LoireSession : GénétiqueParticipation à cette étude : Plateforme d'Infectiologie Expérimentale (PFIE) UE 1277 - Nouzilly Centre Inra Val de Loire; La pasteurellose est la première cause de mortalité des femelles en élevage cunicole. Le projet RELAPA (Génomique pour la REsistance génétique des LApins à la PAsteurellose) a pour objectif de mettre en évidence des régions du génome associées à la réponse à la pasteurellose. Pour ce faire, 955 lapins ont été inoculés à 6 semaines d’âge avec une souche de Pasteurella multocida pyogène et suivis pendant 14 jours. La réponse des animaux était très variable, avec 7% de lapins résistants (sans aucun symptôme de pasteurellose et sans Pasteurella multocida détectée dans les organes) et 11% d’animaux très sensibles (morts ou euthanasiés pendant l’essai avec des Pasteurella multocida détectées). L’effet du père sur les caractères mesurés est très significatif, ce qui laisse présager l’existence d’une variabilité génétique importante dans la résistance à la pasteurellose.

Published
2017

19. Transpariser un organe... et tout devient limpide !

Author

Kévin Etoile, Christelle Rossignol, Maryse Meurisse, Isabelle Lantier, Mathieu Epardaud, Marie-Claire Blache, Xavier Cayla, Elodie Chaillou-Sagon, Frederic Lantier, Infectiologie Animale et Santé Publique - IASP (Nouzilly, France), Institut National de la Recherche Agronomique (INRA), Physiologie de la reproduction et des comportements [Nouzilly] (PRC), Centre National de la Recherche Scientifique (CNRS)-Université de Tours-Institut Français du Cheval et de l'Equitation [Saumur]-Institut National de la Recherche Agronomique (INRA), Plateforme d'Imagerie Cellulaire (PIC), ProdInra, Archive Ouverte, Infectiologie et Santé Publique (UMR ISP), Institut National de la Recherche Agronomique (INRA)-Université de Tours (UT), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS), Institut National de la Recherche Agronomique (INRA)-Université de Tours, Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours-Centre National de la Recherche Scientifique (CNRS), and Association Française d'Histotechnologie (AFH). FRA.

Subjects
mice, mouton, microscopie confocale, [INFO.INFO-IM] Computer Science [cs]/Medical Imaging, quail, microscope confocal, souris, confocal microscopy, caille, lung, réactif de transparisation, ovin, poumon, [INFO.INFO-IM]Computer Science [cs]/Medical Imaging, immunomarquage, wether hoggs, tissu cerebral
Abstract

Transpariser un organe... et tout devient limpide !. Journées d'Animation Scientifique de la Fédération de Recherche d'Infectiologie (FéRI)

Published
2017

20. EU-approved rapid tests might underestimate bovine spongiform encephalopathy infection in goats

Author

Francesco Ingravalle, Daniela Meloni, Romolo Nonno, Silvia Colussi, Danilo Pitardi, Martin H. Groschup, Jan P. M. Langeveld, Lucien van Keulen, Wilfred Goldmann, Theodoros Sklaviadis, Alex Bossers, Isabelle Lantier, Pier Luigi Acutis, Elena Bozzetta, Olivier Andreoletti, Simone Peletto, CEA, Centro di Referenza Nazionale per lo Studio e le Ricerche sulle Encefalopatie Animali e Neuropatologie Comparate, Istituto Zooprofilattico Sperimentale del Piemonte, Part of Wageningen UR, Central Veterinary Institute, Friedrich-Loeffler-Institut (FLI), The Roslin Institute, Interactions hôtes-agents pathogènes [Toulouse] (IHAP), Institut National de la Recherche Agronomique (INRA)-Ecole Nationale Vétérinaire de Toulouse (ENVT), Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées, UR Infectiologie animale et Santé publique (UR IASP), Institut National de la Recherche Agronomique (INRA), Department of Veterinary Public Health and Food Safety, Istituto Superiore di Sanità, Laboratory of Pharmacology, Department of Pharmaceutical Sciences, School of Health Sciences, Aristotle University of Thessaloniki, Grant GoatBSE–Specific Targeted Research Project (FOOD-CT-2006-36353), Biotechnology and Biological Sciences Research Council (BBSRC), Université de Toulouse (UT)-Université de Toulouse (UT)-Institut National Polytechnique (Toulouse) (Toulouse INP), Université de Toulouse (UT)-Université de Toulouse (UT), Infectiologie et Santé Publique (UMR ISP), Institut National de la Recherche Agronomique (INRA)-Université de Tours (UT), Istituto Superiore di Sanità (ISS), and Institut National de la Recherche Agronomique (INRA)-Université de Tours

Subjects
0301 basic medicine, Veterinary medicine, diagnosis, Scrapie, mad cow disease, BSE, 0403 veterinary science, comparaison d'efficacité, rapid tests, Transmissible spongiform encephalopathy, Bacteriologie, Bacteriology, Host Pathogen Interaction & Diagnostics, 04 agricultural and veterinary sciences, 3. Good health, Encephalopathy, Bovine Spongiform, Europe, mother to child transmission, surveillance, Mother to child transmission, goats, 040301 veterinary sciences, chèvre, Bovine spongiform encephalopathy, Enzyme-Linked Immunosorbent Assay, Sensitivity and Specificity, prion, 03 medical and health sciences, medicine, Animals, nannygoats, Host Pathogen Interaction & Diagnostics, Goat Diseases, [SDV.BA.MVSA]Life Sciences [q-bio]/Animal biology/Veterinary medicine and animal Health, General Veterinary, business.industry, scrapie, Bacteriology, test rapide de diagnostic, medicine.disease, transmission maladie, sensitivity, Virology, Host Pathogen Interactie & Diagnostiek, encéphalopathie spongiforme bovine, 030104 developmental biology, Bacteriologie, Host Pathogen Interactie & Diagnostiek, Cattle, Reagent Kits, Diagnostic, rida, business, EU
Abstract

International audience; We report the diagnostic sensitivity of 3 EU-approved rapid tests (ELISAs; 1 from IDEXX and 2 from Bio-Rad) for the detection of transmissible spongiform encephalopathy diseases in goats. Ninety-eight goat brainstem samples were tested. All the rapid tests had 100% specificity and ≥80% sensitivity, with the IDEXX test significantly more sensitive than the 2 Bio-Rad tests. All tests detected 100% of samples from goats with clinical scrapie, but missed 8% (IDEXX) to 33% (Bio-Rad SG) of samples from preclinical goats. Importantly, only IDEXX picked up all samples from clinical bovine spongiform encephalopathy (BSE)-infected goats, whereas the other 2 rapid tests missed 15% (Bio-Rad SG) to 25% (Bio-Rad SAP). These results show that a fraction of preclinical scrapie infections are likely missed by EU surveillance, with sensitivity of detection strongly dependent on the choice of the rapid test. Moreover, a significant proportion of clinical BSE infections are underestimated by using either Bio-Rad test. Assuming that the same sensitivity on preclinical goats would also occur in BSE-infected goats, our data suggest that IDEXX is likely the most sensitive test for detecting preclinical field cases of BSE infection in goats, although with an 8% failure rate. These results raise some concerns about the reliability of current EU surveillance figures on BSE infection in goats.

Published
2017
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21. Protein delivery by porous cationic maltodextrin-based nanoparticles into nasal mucosal cells: Comparison with cationic or anionic nanoparticles

Author

François Fasquelle, Céline Ducournau, Rodolphe Carpentier, Isabelle Dimier-Poisson, Didier Betbeder, Minh Quan Le, Isabelle Lantier, Lille Inflammation Research International Center - U 995 (LIRIC), Centre Hospitalier Régional Universitaire [Lille] (CHRU Lille)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Lille-Institut Pasteur de Lille, Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP), Infectiologie et Santé Publique (UMR ISP), Institut National de la Recherche Agronomique (INRA)-Université de Tours, Université d'Artois (UA), European Project: 607690,EC:FP7:PEOPLE,FP7-PEOPLE-2013-ITN,UNIVACFLU(2013), Institut Pasteur de Lille, Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Lille-Centre Hospitalier Régional Universitaire [Lille] (CHRU Lille), Institut National de la Recherche Agronomique (INRA)-Université de Tours (UT), Chanteloup, Nathalie Katy, and Universal Flu Vaccine - UNIVACFLU - - EC:FP7:PEOPLE2013-11-01 - 2017-10-31 - 607690 - VALID

Subjects
[SDV]Life Sciences [q-bio], lcsh:RS1-441, Pharmaceutical Science, 02 engineering and technology, Endocytosis, Article, lcsh:Pharmacy and materia medica, 03 medical and health sciences, chemistry.chemical_compound, ComputingMethodologies_COMPUTERGRAPHICS, 030304 developmental biology, 0303 health sciences, Liposome, biology, Nasal, Protein delivery, Cationic polymerization, 021001 nanoscience & nanotechnology, Maltodextrin, In vitro, [SDV] Life Sciences [q-bio], PLGA, Ovalbumin, chemistry, [SDV.SP.PHARMA] Life Sciences [q-bio]/Pharmaceutical sciences/Pharmacology, [SDV.SP.PHARMA]Life Sciences [q-bio]/Pharmaceutical sciences/Pharmacology, Biophysics, biology.protein, Nanoparticles, Nanocarriers, 0210 nano-technology, Vaccine
Abstract

Graphical abstract, Different types of biodegradable nanoparticles (NPs) have been studied as delivery systems for proteins into nasal mucosal cells, especially for vaccine applications. Such a nanocarrier must have the ability to be loaded with proteins and to transport this payload into mucosal cells. However, comparative data on nanoparticles’ capacity for protein loading, efficiency of subsequent endocytosis and the quantity of nanocarriers used are either lacking or contradictory, making comparisons and the choice of a best candidate difficult. Here we compared 5 types of nanoparticles with different surface charge (anionic or cationic) and various inner compositions as potential vectors: the NPL (cationic maltodextrin NP with an anionic lipid core), cationic and anionic PLGA (Poly Lactic co-Glycolic Acid) NP, and cationic and anionic liposomes. We first quantified the protein association efficiency and NPL associated the largest amount of ovalbumin, used as a model protein. In vitro, the delivery of fluorescently-labeled ovalbumin into mucosal cells (airway epithelial cells, dendritic cells and macrophages) was assessed by flow cytometry and revealed that the NPL delivered protein to the greatest extent in all 3 different cell lines. Taken together, these data underlined the potential of the porous and cationic maltodextrin-based NPL as efficient protein delivery systems to mucosal cells.

Published
2019
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22. Protecting effect of PrP codons M142 and K222 in goats orally challenged with bovine spongiform encephalopathy prions

Author

Jan P. M. Langeveld, Nora Hunter, Martin H. Groschup, Patricia Berthon, Christelle Rossignol, Alex Bossers, Christine Fast, J. G. Jacobs, Isabelle Lantier, Frédéric Lantier, Olivier Andreoletti, Kerstin Tauscher, Wilfred Goldmann, Friedrich-Loeffler-Institut (FLI), The Roslin Institute, Infectiologie Santé Publique (ISP-311), Institut National de la Recherche Agronomique (INRA)-Université de Tours, Interactions hôtes-agents pathogènes [Toulouse] (IHAP), Institut National de la Recherche Agronomique (INRA)-Ecole Nationale Vétérinaire de Toulouse (ENVT), Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées, Wageningen BioVeterinary Research, Infectiologie et Santé Publique (UMR ISP), Institut National de la Recherche Agronomique (INRA)-Université de Tours (UT), Wageningen University and Research [Wageningen] (WUR), and European project, Dutch Ministry of Economic Affairs project, Biotechnology and Biological Sciences Research Council (strategic programme Grant)

Subjects
0301 basic medicine, Male, [SDV]Life Sciences [q-bio], animal diseases, Scrapie, polymorphisme, Breeding, Polymorphism (computer science), Genotype, Disease Resistance, 2. Zero hunger, Animal biology, lcsh:Veterinary medicine, Goat Diseases, Goats, Bacteriologie, Bacteriology, Host Pathogen Interaction & Diagnostics, Encephalopathy, Bovine Spongiform, Female, génotype, Research Article, mouton, Prions, chèvre, Bovine spongiform encephalopathy, Biology, Prion Proteins, Incubation period, PRNP, 03 medical and health sciences, maladie à prion, Biologie animale, Journal Article, medicine, Life Science, Animals, Host Pathogen Interaction & Diagnostics, General Veterinary, Wild type, Bacteriology, medicine.disease, Virology, Host Pathogen Interactie & Diagnostiek, encéphalopathie spongiforme bovine, nervous system diseases, 030104 developmental biology, Bacteriologie, Host Pathogen Interactie & Diagnostiek, lcsh:SF600-1100, Cattle, codon
Abstract

Breeding towards genetic resistance to prion disease is effective in eliminating scrapie. In sheep, classical forms of scrapie have been eradicated almost completely in several countries by breeding programs using a prion protein (PrP) gene (PRNP) amino acid polymorphism. For goats, field and experimental studies have provided evidence for several amino acid polymorphisms that are associated with resistance to scrapie, but only limited data are available concerning the susceptibility of caprine PRNP genotypes to BSE. In this study, goat kids representing five PRNP genotypes based on three polymorphisms (M142, Q211 and K222 and the wild type I142, R211 and Q222) were orally challenged with bovine or goat BSE. Wild type goats were killed with clinical signs between 24–28 months post inoculation (mpi) to both challenges, and goats with genotype R/Q211 succumbed between 29–36 mpi. I/M142 goats developed clinical signs at 44–45 mpi and M/M142 goats remained healthy until euthanasia at 48 mpi. None of the Q/K222 goats showed definite clinical signs. Taken together the highest attack ratios were seen in wild type and R/Q211 goats, and the lowest in I/M142, M/M142 and Q/K222. In all genotype groups, one or more goats remained healthy within the incubation period in both challenges and without detectable PrP deposition in the tissues. Our data show that both the K222 and M142 polymorphisms lengthen the incubation period significantly compared to wild type animals, but only K222 was associated with a significant increase in resistance to BSE infection after oral exposure to both BSE sources. Electronic supplementary material The online version of this article (doi:10.1186/s13567-017-0455-0) contains supplementary material, which is available to authorized users.

Published
2017
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23. La transparisation d'organes : une approche innovante pour l'analyse en 3D d'immunomarquages fluorescents

Author

Patricia Berthon, Maryse Meurisse, Mathieu Epardaud, Isabelle Dimier-Poisson, Isabelle Lantier, Raymond Nowak, Frederic Lantier, Infectiologie et Santé Publique (UMR ISP), Institut National de la Recherche Agronomique (INRA)-Université de Tours (UT), Physiologie de la reproduction et des comportements [Nouzilly] (PRC), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS), Institut National de la Recherche Agronomique (INRA), Fédération de recherche en infectiologie (FéRI)., ProdInra, Migration, Institut National de la Recherche Agronomique (INRA)-Université de Tours, and Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours-Centre National de la Recherche Scientifique (CNRS)

Subjects
[SDV.BA] Life Sciences [q-bio]/Animal biology, [SDV.BA]Life Sciences [q-bio]/Animal biology, ComputingMilieux_MISCELLANEOUS
Abstract

National audience

Published
2016

24. Transmissions and Strains

Author

Markus Keller, Frédéric Lantier, Martin Eiden, Christine Fast, Patricia Berthon, Isabelle Lantier, Olivier Andreoletti, and Groschup, Martin, H.

Subjects
Genetically modified mouse, Cellular and Molecular Neuroscience, Infectious Diseases, Scrapie, Cell Biology, Biology, Biochemistry, Virology, Phenotype, Co infection
Published
2012
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25. Quantitative trait loci for resistance to infection in sheep using a live Salmonella Abortusovis vaccine

Author

Guillaume Sallé, R. Boivin, Edmond P. Cribiu, Isabelle Lantier, Patricia Berthon, Jean Louis Weisbecker, Laurent Schibler, Dominique François, Jacques Bouix, Frédérique Pitel, Jean-Michel Elsen, Carole Moreno, Frédéric Lantier, and A.V. Gautier-Bouchardon

Subjects
Genetics, SLC11A1, 0303 health sciences, Salmonella, biology, Strain (biology), Salmonella Abortusovis, Sire, 0402 animal and dairy science, 04 agricultural and veterinary sciences, General Medicine, Quantitative trait locus, medicine.disease_cause, Major histocompatibility complex, 040201 dairy & animal science, 03 medical and health sciences, medicine, biology.protein, Animal Science and Zoology, Chromosome 20, 030304 developmental biology
Abstract

Quantitative trait loci (QTL) mapping for susceptibility to a Salmonella Abortusovis vaccinal strain was performed using an experimental design involving 30 Romane sheep sire families (1216 progenies). Nine QTL corresponding to bacterial load, weight variations and antibody response criteria were mapped on eight chromosomes, including the major histocompatibility complex area on chromosome 20. Surprisingly, none was found to be significant in the SLC11A1 region (formerly NRAMP1) that has been shown to influence Salmonella susceptibility in other species.

Published
2012
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26. Proteinase K-Resistant Material in ARR/VRQ Sheep Brain Affected with Classical Scrapie Is Composed Mainly of VRQ Prion Protein

Author

Theodoros Sklaviadis, Human Rezaei, J. G. Jacobs, F.G. van Zijderveld, Patricia Berthon, Alex Bossers, Jan P. M. Langeveld, Isabelle Lantier, Frédéric Lantier, Sandra McCutcheon, L.J.M. van Keulen, Wageningen University and Research Centre (WUR), Unité de recherche Virologie et Immunologie Moléculaires (VIM (UR 0892)), Institut National de la Recherche Agronomique (INRA), The Roslin Institute, Aristotle University of Thessaloniki, Infectiologie Animale et Santé Publique (UR IASP), and These investigations were largely supported by the Dutch Ministry of Economic Affairs, Agriculture and Innovation, projects WOT-01-002-001.01 and WOT-01-002-001.05. Experimental BSE and scrapie samples were generated within EU project BSE in sheep (QLRT-2001-01309), led by Olivier Andreoletti, INRA-Toulouse-ENVT. Antibody characterization of FH6 was supported by the United Kingdom-Netherlands Partnership Programme in Science.

Subjects
Epidemiology, animal diseases, Drug Resistance, Scrapie, Immunoenzyme Techniques, 0403 veterinary science, in-vitro conversion, Genotype, Electrophoresis, Gel, Two-Dimensional, natural scrapie, prp, 0303 health sciences, education.field_of_study, biology, Bacteriologie, Brain, Bacteriology, Host Pathogen Interaction & Diagnostics, straussler-scheinker disease, 04 agricultural and veterinary sciences, Flow Cytometry, allelic variants, [SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology, susceptibility-linked polymorphisms, Disease Susceptibility, Endopeptidase K, Antibody, Bioinformatica & Diermodellen, Prions, 040301 veterinary sciences, Bovine spongiform encephalopathy, monoclonal-antibodies, Blotting, Western, Immunology, Population, Microbiology, prion, 03 medical and health sciences, Virology, Bio-informatics & Animal models, medicine, Animals, Epidemiology, Bio-informatics & Animal models, bovine spongiform encephalopathy, Allele, Allelotype, education, creutzfeldt-jakob-disease, Alleles, 030304 developmental biology, Host Pathogen Interaction & Diagnostics, Epidemiologie, Sheep, Structure and Assembly, Bacteriology, Proteinase K, medicine.disease, Molecular biology, Host Pathogen Interactie & Diagnostiek, nervous system diseases, Epidemiologie, Bioinformatica & Diermodellen, Insect Science, Bacteriologie, Host Pathogen Interactie & Diagnostiek, biology.protein, amino-acid, genetic
Abstract

Classical scrapie is a prion disease in sheep and goats. In sheep, susceptibility to disease is genetically influenced by single amino acid substitutions. Genetic breeding programs aimed at enrichment of arginine-171 (171R) prion protein (PrP), the so-called ARR allele, in the sheep population have been demonstrated to be effective in reducing the occurrence of classical scrapie in the field. Understanding the molecular basis for this reduced prevalence would serve the assessment of ARR adaptation. The prion formation mechanism and conversion of PrP from the normal form (PrP C ) to the scrapie-associated form (PrP Sc ) could play a key role in this process. Therefore, we investigated whether the ARR allele substantially contributes to scrapie prion formation in naturally infected heterozygous 171Q/R animals. Two methods were applied to brain tissue of 171Q/R heterozygous sheep with natural scrapie to determine the relative amount of the 171R PrP fraction in PrP res , the proteinase K-resistant PrP Sc core. An antibody test differentiating between 171Q and 171R PrP fragments showed that PrP res was mostly composed of the 171Q allelotype. Furthermore, using a novel tool for prion research, endoproteinase Lys-C-digested PrP res yielded substantial amounts of a nonglycosylated and a monoglycosylated PrP fragment comprising codons 114 to 188. Following two-dimensional gel electrophoresis, only marginal amounts (res were detected. Enhanced 171R res proteolytic susceptibility could be excluded. Thus, these data support a nearly zero contribution of 171R PrP in PrP res of 171R/Q field scrapie-infected animals. This is suggestive of a poor adaptation of classical scrapie to this resistance allele under these natural conditions.

Published
2011
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27. Mechanisms allowing protein delivery in nasal mucosa using NPL nanoparticles

Author

Rodolphe Carpentier, Isabelle Lantier, Isabelle Dimier-Poisson, Didier Betbeder, B. Bernocchi, Céline Ducournau, Lille Inflammation Research International Center - U 995 (LIRIC), Centre Hospitalier Régional Universitaire [Lille] (CHRU Lille)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Lille-Institut Pasteur de Lille, Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP), Université de Lille, Centre Hospitalier Régional Universitaire [Lille] (CHRU Lille), Infectiologie et Santé Publique (UMR ISP), Institut National de la Recherche Agronomique (INRA)-Université de Tours, Université d'Artois (UA), (Marie Curie Actions) of the European Union Seventh Framework Programme FP7/2007-2013/ under REA grant agreement no. [607690], Lille Inflammation Research International Center (LIRIC), Institut Pasteur de Lille, Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Lille-Centre Hospitalier Régional Universitaire [Lille] (CHRU Lille), Infectiologie Animale et Santé Publique - IASP (Nouzilly, France), Institut National de la Recherche Agronomique (INRA), and Institut National de la Recherche Agronomique (INRA)-Université de Tours (UT)

Subjects
0301 basic medicine, nasal mucosa, Pharmaceutical Science, 02 engineering and technology, muqueuse nasale, Intranasal drug delivery, nanoparticule, Mice, Biodistribution, Medicine, Drug Carriers, biology, nanoparticle, 021001 nanoscience & nanotechnology, Endocytosis, 3. Good health, Cell biology, Transcytosis, protéine, Epichlorohydrin, 0210 nano-technology, Drug carrier, Ovalbumin, Article, Permeability, 03 medical and health sciences, Immune system, Polysaccharides, In vivo, Animals, Antigens, Administration, Intranasal, business.industry, Protein delivery, In vitro, Quaternary Ammonium Compounds, 030104 developmental biology, administration intra nasal, Immunology, biology.protein, Epoxy Compounds, Nanoparticles, Nasal administration, business, protein, Vaccine, [SDV.MHEP]Life Sciences [q-bio]/Human health and pathology
Abstract

The intranasal administration of proteins using nanoparticles is a promising approach for several applications, especially for mucosal vaccines. Delivery of protein within the epithelial barrier is a key point to elicit an immune response and nano-carrier has to show no toxicity. The aim of this work was to elucidate the interactions of cationic porous nanoparticles loaded with protein delivery for antigen delivery in the nose. We investigated the loading, the cellular delivery and the epithelial transcytosis of proteins associated to these nanoparticles containing an anionic lipid in their core (NPL). NPL were highly endocytosed by airway epithelial cells and significantly improved the protein delivery into the cell. In vitro transcytosis studies showed that NPL did not modify the in vitro epithelial permeability suggesting no toxicity of these carriers. Moreover protein and NPL did not translocate the epithelial barrier. In vivo studies demonstrated that NPL prolonged the nasal residence time of the protein and no NPL were found beyond the epithelial barrier in vivo, precluding a negative side effect. All together these results establish the NPL as a bio-eliminable and optimal vaccine carrier., Graphical abstract After nasal administration, NPL deliver antigens in airway epithelial cells without crossing the epithelial barrier.Image 1

Published
2016
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28. Quantitative trait loci linked to PRNP gene controlling health and production traits in INRA 401 sheep

Author

Zulma G. Vitezica, Jacques Bouix, Jean-Michel Elsen, Carole Moreno, Francis Barillet, Anne Roig, Daniel Allain, Dominique François, Laurent Schibler, Frédéric Lantier, J. C. Brunel, Isabelle Lantier, Station d'Amélioration Génétique des Animaux (SAGA), Institut National de la Recherche Agronomique (INRA), Centre Hospitalier Universitaire de Purpan (CHU Purpan), Infectiologie Animale et Santé Publique (UR IASP), Unité de recherche Génétique Biochimique et Cytogénétique (LGBC), and Domaine expérimental Bourges-La Sapinière (BOURGES)

Subjects
association analysis, production trait, health trait, gene prnp, sheep, Linkage disequilibrium, Time Factors, [SDV]Life Sciences [q-bio], animal diseases, Health Status, Genotype, Genetics(clinical), ComputingMilieux_MISCELLANEOUS, lcsh:SF1-1100, 2. Zero hunger, Genetics, 0303 health sciences, education.field_of_study, Reproduction, Wool, 04 agricultural and veterinary sciences, General Medicine, Breed, Female, lcsh:QH426-470, gene PRNP, Prions, Population, Quantitative Trait Loci, Quantitative trait locus, Biology, PRNP, 03 medical and health sciences, Animals, Genetic Predisposition to Disease, Allele, education, Ecology, Evolution, Behavior and Systematics, 030304 developmental biology, Genetic association, Research, 0402 animal and dairy science, 040201 dairy & animal science, nervous system diseases, [SDV.GEN.GA]Life Sciences [q-bio]/Genetics/Animal genetics, lcsh:Genetics, Animal Science and Zoology, lcsh:Animal culture
Abstract

In this study, the potential association of PrP genotypes with health and productive traits was investigated. Data were recorded on animals of the INRA 401 breed from the Bourges-La Sapinière INRA experimental farm. The population consisted of 30 rams and 852 ewes, which produced 1310 lambs. The animals were categorized into three PrP genotype classes: ARR homozygous, ARR heterozygous, and animals without any ARR allele. Two analyses differing in the approach considered were carried out. Firstly, the potential association of the PrP genotype with disease (Salmonella resistance) and production (wool and carcass) traits was studied. The data used included 1042, 1043 and 1013 genotyped animals for the Salmonella resistance, wool and carcass traits, respectively. The different traits were analyzed using an animal model, where the PrP genotype effect was included as a fixed effect. Association analyses do not indicate any evidence of an effect of PrP genotypes on traits studied in this breed. Secondly, a quantitative trait loci (QTL) detection approach using the PRNP gene as a marker was applied on ovine chromosome 13. Interval mapping was used. Evidence for one QTL affecting mean fiber diameter was found at 25 cM from the PRNP gene. However, a linkage between PRNP and this QTL does not imply unfavorable linkage disequilibrium for PRNP selection purposes.

Published
2007

29. Fast, reversible interaction of prion protein with RNA aptamers containing specific sequence patterns

Author

Daniel Marc, M.-C. Maurel, Isabelle Lantier, Jeanne Grosclaude, R. Mercey, Frédéric Lantier, Infectiologie Animale et Santé Publique (UR IASP), Institut National de la Recherche Agronomique (INRA), Physiologie de la reproduction et des comportements [Nouzilly] (PRC), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS), Unité de recherche Virologie et Immunologie Moléculaires (VIM (UR 0892)), and Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours-Centre National de la Recherche Scientifique (CNRS)

Subjects
Protein Conformation, animal diseases, Aptamer, Molecular Sequence Data, Scrapie, Biology, Structure-Activity Relationship, 03 medical and health sciences, Protein structure, Virology, Animals, PrPC Proteins, Binding site, 030304 developmental biology, 0303 health sciences, Sheep, Base Sequence, SELEX Aptamer Technique, 030302 biochemistry & molecular biology, RNA, General Medicine, Aptamers, Nucleotide, nervous system diseases, Biochemistry, [SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology, Nucleic acid, Nucleic Acid Conformation, Peptides, Systematic evolution of ligands by exponential enrichment, Protein Binding
Abstract

International audience; One of the unsolved problems in prion diseases relates to the physiological function of cellular prion protein (PrP), of which a misfolded isoform is the major component of the transmissible spongiform encephalopathies agent. Knowledge of the PrP-binding molecules may help in elucidating its role and understanding the pathological events underlying prion diseases. Because nucleic acids are known to bind PrP, we attempted to identify the preferred RNA sequences that bind to the ovine recombinant PrP. An in vitro selection approach (SELEX) was applied to a pool of 80-nucleotide(nt)-long RNAs containing a randomised 40-nt central region. The most frequently isolated aptamer, RM312, was also the best ligand (20 nM KD value), according to both surface plasmon resonance and filter binding assays. The fast rates of association and dissociation of RM312 with immobilized PrP, which are reminiscent of biologically relevant interactions, could point to a physiological function of PrP towards cellular nucleic acids. The minimal sequence that we found necessary for binding of RM312 to PrP presents a striking similarity with one previously described PrP aptamer of comparable affinity. In addition, we here identify the two lysine clusters contained in the N-terminal part of PrP as its main nucleic-acid binding sites.

Published
2006
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30. Réponse immunitaire à la eCG utilisée dans le traitement de l’induction d’ovulation chez la chèvre et la brebis

Author

Eduardo Manfredi, Daniel Vaiman, Isabelle Lantier, M. C. Maurel, Edmond-Paul Cribiu, P Boue, Florian Guillou, F. Roy, F Bouvier, V Hervé, and J. Bertin

Subjects
Estrous cycle, medicine.medical_specialty, biology, Artificial insemination, medicine.medical_treatment, media_common.quotation_subject, Obstetrics and Gynecology, General Medicine, Insemination, Andrology, Immune system, Endocrinology, Reproductive Medicine, Internal medicine, medicine, biology.protein, Antibody, Equine chorionic gonadotropin, Ovulation, media_common, Hormone
Abstract

In dairy goats and ewes the use of equine Chorionic Gonadotropin (eCG) as a convenient hormone for the induction of ovulation is necessary for out-of-season breeding and artificial insemination (AI). Treatment for induction and synchronization of ovulation consists of a progestagen delivered by vaginal sponge, followed by an eCG injection. In some females, the first injection of eCG induces a humoral response with high concentrations of anti-eCG antibodies in contrast to other females displaying a very low concentration of anti-eCG antibodies. Females eliciting a low response were also poor responders after the following treatments. Conversely, high responders at the first treatment systematically yielded high immune responses upon the following treatment. By a molecular genetic approach using microsatellites we showed that the anti-eCG immune response phenotypes were associated with MHC class II polymorphism. Females with high residual antibody concentrations at the time of eCG injection exhibited a much lower kidding rate than other females did. Lower fertility of these females, inseminated at a fixed time after eCG treatment (43H for goats and 55H for ewes), might be due to the delay in estrus occurrence and the pre ovulatory LH surge. Consequently, under field conditions old females selected for AI are only those with low residual anti-eCG antibody concentrations and old females with high residual antibody concentration are culled from AI breeding because of their low fertility during the previous year. So we have undertaken comparative studies to establish if the anti-eCG immune response is correlated with the global immunity in animals.

Published
2003
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31. An ancient interlocus recombination increases class II MHC DQA diversity in sheep and other[i] Bovidae[/i]

Author

Guido Lopez, Isabelle Lantier, Philip Steele, Matteo Cotelli, Keith T. Ballingall, Frédéric Lantier, E. Martin, Helen Todd, Moredun Research Institute, UR Infectiologie animale et Santé publique (UR IASP), Institut National de la Recherche Agronomique (INRA), Scottish Government, European Community FP7-228394 (NADIR), Moredun Research Institute [Penicuik, UK] (MRI), Infectiologie et Santé Publique (UMR ISP), Institut National de la Recherche Agronomique (INRA)-Université de Tours, and Institut National de la Recherche Agronomique (INRA)-Université de Tours (UT)

Subjects
endocrine system diseases, Genes, MHC Class II, Molecular Sequence Data, Locus (genetics), Major histocompatibility complex, 03 medical and health sciences, 0302 clinical medicine, Phylogenetics, Genetics, Animals, Amino Acid Sequence, Allele, Alleles, Phylogeny, Sheep, Domestic, 030304 developmental biology, Recombination, Genetic, 0303 health sciences, MHC class II, Phylogenetic tree, biology, Goats, [SDV.BA]Life Sciences [q-bio]/Animal biology, Haplotype, goat, DQA2-like, Sequence Analysis, DNA, General Medicine, Biological Evolution, Histocompatibility, ovine, Haplotypes, cattle, biology.protein, Animal Science and Zoology, 030215 immunology
Abstract

Animals with fully characterised major histocompatibility complex (MHC) regions are often used to explore the molecular interactions that control the induction of adaptive immunity. The ovine MHC includes two DQA loci, termed DQA1 and DQA2. However, in a minority of haplotypes the DQA1 locus appears absent (DQA1 null) and is replaced by a second locus termed, DQA2-like. This raises a number of questions regarding the origins and function of the DQA2-like sequences. To address this, we have analysed DQA diversity associated with 10 MHC haplotypes, including two classified as DQA1 null. Pair-wise comparison between full-length DQA transcripts from each haplotype identified unique diversity throughout the DQA2-like sequences. Conserved orthologues of the DQA2-like sequences were identified in cattle and goat, and phylogenetic analysis clustered exons 1 and 2 with DQA2 whereas the remainder of the sequence clustered with DQA1. The DQA2-like allelic lineage appears functional and to have arisen from an ancient interlocus recombination between DQA1 and DQA2 loci which predates Bovidae speciation.

Published
2015
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32. Regional characterization of a hamster–sheep somatic cell hybrid panel

Author

Daniel Vaiman, Edmond-Paul Cribiu, N. Saïdi-Mehtar, Anne Oustry-Vaiman, Isabelle Lantier, Frédéric Lantier, Laurent Schibler, Kamila Tabet-Aoul, Unité de Pathologie Infectieuse et Immunologie [Nouzilly] (PII), Institut National de la Recherche Agronomique (INRA), Unité de recherche Génétique Biochimique et Cytogénétique (LGBC), and ProdInra, Migration

Subjects
Genetic Markers, Male, Genetics, [SDV.GEN]Life Sciences [q-bio]/Genetics, Sheep, Somatic cell, Goats, Chromosome Mapping, Hamster, [SDV.GEN] Life Sciences [q-bio]/Genetics, DNA, Computational biology, Hybrid Cells, Biology, Polymerase Chain Reaction, Gene mapping, Genetic marker, Cricetinae, Animals, Cattle, Female, ComputingMilieux_MISCELLANEOUS, Synteny
Abstract

The regional characterization of a previously obtained hamster-sheep hybrid panel is reported. Using data available from ruminant maps (sheep, cattle, and goat), we have selected a set of 300 markers and have analyzed them by PCR in this hybrid panel. Results obtained for 204 markers show the presence of all sheep chromosomes (including gonosomes) in entire or fragmented form. Analysis of syntenies has given 130 types of answer defining segments of variable sizes. This study has led to the regional characterization of this panel and provides comparative data on a set of bovine and caprine markers. With the level of characterization now achieved for this hybrid panel, the regional assignment of new genes or markers to sheep chromosomes can be rapidly obtained. Finally, this panel will help to collect new data for comparative mapping of domestic animals and to highlight the conservation of syntenic groups between closely related species, that is, sheep, cattle, and goat.

Published
2000
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33. The Negative Effect of Repeated Equine Chorionic Gonadotropin Treatment on Subsequent Fertility in Alpine Goats Is Due to a Humoral Immune Response Involving the Major Histocompatibility Complex1

Author

Isabelle Lantier, Marie Christine Maurel, François Roy, Daniel Vaiman, Florian Guillou, Edmond P. Cribiu, Yves Combarnous, Bruno Combes, François Delétang, and Thierry Pobel

Subjects
Estrous cycle, Infertility, medicine.medical_specialty, biology, medicine.drug_class, media_common.quotation_subject, Artificial insemination, medicine.medical_treatment, Cell Biology, General Medicine, medicine.disease, Andrology, Endocrinology, Immune system, Reproductive Medicine, Internal medicine, medicine, biology.protein, Antibody, Gonadotropin, Equine chorionic gonadotropin, Ovulation, media_common
Abstract

In dairy goats, the use of eCG as a convenient hormone for the induction of ovulation is necessary for out-of-season breeding and artificial insemination. However, repeated eCG treatments are followed by decreased fertility in goats inseminated at a fixed time after treatment. In this report, we show the presence of anti-eCG antibodies in plasma of treated goats. A 500 IU eCG injection induces a humoral response, with variable concentrations of anti-eCG antibody being produced in individual goats. The analysis of successive anti-eCG immune responses over several years has demonstrated the existence of different populations of goats, defined as low, medium, and high responders. By the use of two caprine microsatellites located inside (OLADRB) and outside (BM1258) the major histocompatibility complex (MHC), a significant association (p 2.5 microg/ml) exhibited a much lower kidding rate than did other females (41.3% vs. 66.7%). Lower fertility of these goats, inseminated at a fixed time after eCG treatment, might be due to the observed delay in estrus occurrence and the preovulatory LH surge.

Published
1999
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34. cDNA cloning, structural organization, and expression of the sheep NRAMP1 gene

Author

Sylvie Patri, Philippe Gros, F. Nau, Jean Michel Elsen, Isabelle Lantier, Frédéric Lantier, Frédérique Pitel, and Vincent Bussmann

Subjects
DNA, Complementary, Transcription, Genetic, Molecular Sequence Data, Gene Expression, Biology, Primer extension, Mice, Exon, Complementary DNA, parasitic diseases, Genetics, Animals, Humans, Tissue Distribution, Amino Acid Sequence, RNA, Messenger, Cloning, Molecular, Cation Transport Proteins, Gene, Genomic organization, Messenger RNA, Sheep, Base Sequence, Sequence Homology, Amino Acid, cDNA library, Intron, Membrane Proteins, Exons, Sequence Analysis, DNA, Molecular biology, Introns, Genes, Cattle, Carrier Proteins, Chickens, Sequence Alignment
Abstract

Mouse resistance to several intracellular pathogens including Mycobacteria, Leishmania, and Salmonella is under the control of the Chromosome (Chr) 1 Natural Resistance Associated Macrophage Protein I gene (Nramp1). This gene could have an economic and health importance for domestic animals and humans as well. Therefore, equivalents of the NRAMP1 gene have been cloned by several research groups in various animal species. To study in sheep the influence of the NRAMP1 gene on the susceptibility to intracellular pathogens induced diseases, we have cloned the sheep NRAMP1 cDNA by screening a splenic cDNA library. The genomic organization of the sheep NRAMP1 gene was then determined by sequencing the exon/intron boundaries. The transcription start points (tsp) from the NRAMP1 mRNA have been located with primer extension experiments. RT-PCR reactions have been used to determine the profile of mRNA expression of this gene.

Published
1998
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35. Analysis of the immune response in sheep efferent lymph during Salmonella abortusovis infection

Author

Michel Olivier, I. Gohin, Isabelle Lantier, Frédéric Lantier, M. Pépin, ProdInra, Migration, Unité de Pathologie Infectieuse et Immunologie [Nouzilly] (PII), and Institut National de la Recherche Agronomique (INRA)

Subjects
Male, [SDV]Life Sciences [q-bio], medicine.medical_treatment, T cell, Immunology, Biology, Immunophenotyping, Microbiology, 03 medical and health sciences, 0302 clinical medicine, Immune system, Prescapular Lymph Node, parasitic diseases, medicine, Animals, RNA, Messenger, Lymph node, ComputingMilieux_MISCELLANEOUS, B cell, 030304 developmental biology, Salmonella Infections, Animal, 0303 health sciences, Sheep, General Veterinary, Lymphoblast, Histocompatibility Antigens Class II, Antibodies, Bacterial, [SDV] Life Sciences [q-bio], medicine.anatomical_structure, Cytokine, biology.protein, Cytokines, Lymph, Antibody, human activities, REPONSE IMMUNE, 030215 immunology
Abstract

The efferent lymph duct of the ovine prescapular lymph node was cannulated, and Salmonella abortusovis (SAO), a specific pathogen for sheep inducing abortion and mortality of newborn lambs, was inoculated by the subcutaneous route in this lymph node drained area. While the prescapular lymph node draining the inoculation site represented an efficient barrier for the vaccinal SAO Rv6 strain spreading, SAO 15 5 virulent bacteria were steadily detected in efferent lymph of infected sheep. The inoculation of the virulent strain of SAO induced a greater increase of the cell output than did the attenuated vaccinal strain, but proportions of blast cells appearing in the efferent lymph were similar in both cases. Flow cytometry analysis showed that B and T cell outputs were both increased during SAO infections, but while T cell subset proportions slightly decreased, B cell percentages significantly rose, and, at the peak response, almost all of the lymphoblast cells were activated B cells. Typical antibody profiles characteristic of a primary immune response were observed, and antibody titres were greater in the efferent lymph of animals inoculated with the virulent strain of SAO. Many of the cytokine mRNAs we investigated were steadily detected by RT-PCR in efferent lymph cells of control sheep, but frequencies of detection of IL-2, IFNγ, IL-1β and TNFα mRNAs were augmented in efferent lymph cells following inoculation of both SAO virulent or vaccinal strains. IL-10 and IL-8 mRNAs could only be detected after a SAO inoculation, while detection of IL-4 mRNAs was increased only in efferent lymph cells from SAO virulent strain-infected sheep. The efferent lymph cannulation technique thus appeared a very powerful way to study the in vivo development of the immune response to SAO, in its natural host, the sheep.

Published
1997
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36. Prionemia and leukocyte-platelet-associated infectivity in sheep transmissible spongiform encephalopathy models

Author

Fabien Corbière, Hugh Simmons, Frédéric Lantier, Natalia Fernández-Borges, Séverine Lugan, Jean-Louis Weisbecker, Jacques Grassi, Isabelle Lantier, Didier Vilette, Stéphanie Simon, Pierrette Costes, Caroline Lacroux, Nathalie Morel, François Schelcher, Claire Litaise, Joaquín Castilla, Olivier Andreoletti, Interactions hôtes-agents pathogènes [Toulouse] (IHAP), Institut National de la Recherche Agronomique (INRA)-Ecole Nationale Vétérinaire de Toulouse (ENVT), Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées, Parque Tecnologico de Bizkaia, Commissariat à l'énergie atomique et aux énergies alternatives (CEA), VLA Weybridge, Domaine expérimental de Langlade (LANGLADE), Institut National de la Recherche Agronomique (INRA), Infectiologie et Santé Publique (UMR ISP), Institut National de la Recherche Agronomique (INRA)-Université de Tours, Ikerbasque - Basque Foundation for Science, Université de Toulouse (UT)-Université de Toulouse (UT)-Institut National Polytechnique (Toulouse) (Toulouse INP), Université de Toulouse (UT)-Université de Toulouse (UT), Institut National de la Recherche Agronomique (INRA)-Université de Tours (UT), and Andreoletti, Olivier

Subjects
Blood Platelets, sheep, Blood transfusion, Prions, 040301 veterinary sciences, medicine.medical_treatment, Virologie, animal diseases, Immunology, encephalopathy, Mice, Transgenic, Scrapie, Biology, amplification, Microbiology, Peripheral blood mononuclear cell, Prion Diseases, 0403 veterinary science, Pathogenesis, Mice, 03 medical and health sciences, Virology, infectiosite, medicine, sang, Animals, Humans, Platelet, leucocyte, encéphalopathie spongiforme transmissible, 030304 developmental biology, Infectivity, prp, 0303 health sciences, Transmissible spongiform encephalopathy, 04 agricultural and veterinary sciences, medicine.disease, nervous system diseases, Disease Models, Animal, Insect Science, [SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology, Leukocytes, Mononuclear, Protein Misfolding Cyclic Amplification, protéine prion
Abstract

The dynamics of the circulation and distribution of transmissible spongiform encephalopathy (TSE) agents in the blood of infected individuals remain largely unknown. This clearly limits the understanding of the role of blood in TSE pathogenesis and the development of a reliable TSE blood detection assay. Using two distinct sheep scrapie models and blood transfusion, this work demonstrates the occurrence of a very early and persistent prionemia. This ability to transmit disease by blood transfusion was correlated with the presence of infectivity in white blood cells (WBC) and peripheral blood mononucleated cells (PBMC) as detected by bioassay in mice overexpressing the ovine prion protein PrP (tg338 mice) and with the identification of abnormal PrP in WBC after using protein misfolding cyclic amplification (PMCA). Platelets and a large variety of leukocyte subpopulations also were shown to be infectious. The use of endpoint titration in tg338 mice indicated that the infectivity in WBC (per ml of blood) was 10 6.5 -fold lower than that in 1 g of posterior brainstem sample. In both WBC and brainstem, infectivity displayed similar resistance to PK digestion. The data strongly support the concept that WBC are an accurate target for reliable TSE detection by PMCA. The presence of infectivity in short-life-span blood cellular elements raises the question of the origin of prionemia.

Published
2012
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37. Copy number variation and differential expression of a protective endogenous retrovirus in sheep

Author

Christine Dolmazon, Christophe Terzian, Frédéric Lantier, Caroline Leroux, Barbara Viginier, Fabienne Archer, Isabelle Lantier, Terzian, Christophe, Rétrovirus et Pathologie Comparée, Institut National de la Recherche Agronomique (INRA)-École pratique des hautes études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Ecole Nationale Vétérinaire de Lyon (ENVL)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS), École pratique des hautes études (EPHE), Université Paris sciences et lettres (PSL), Infectiologie et Santé Publique (UMR ISP), Institut National de la Recherche Agronomique (INRA)-Université de Tours (UT), and Institut National de la Recherche Agronomique (INRA)-Université de Tours

Subjects
Virologie, lcsh:Medicine, Endogenous retrovirus, medicine.disease_cause, Lung and Intrathoracic Tumors, Proviruses, Molecular Cell Biology, Inbreeding, RNA, Neoplasm, Copy-number variation, lcsh:Science, Lung, Genome Evolution, 0303 health sciences, Mutation, Genome, Multidisciplinary, Adenocarcinoma of the Lung, DNA, Neoplasm, Genomics, Jaagsiekte sheep retrovirus, Host-Pathogen Interaction, Retrotransposons, Oncology, rétrovirus endogène, Pulmonary Adenomatosis, Ovine, [SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology, Medicine, RNA extraction, Transposons, Research Article, Gene Expression Regulation, Viral, DNA Copy Number Variations, mouton, Mechanisms of Resistance and Susceptibility, Molecular Sequence Data, Gene Products, gag, Biology, analyse phylogénétique, Microbiology, 03 medical and health sciences, Genome Analysis Tools, Complementary DNA, Virology, medicine, Animals, 030304 developmental biology, Evolutionary Biology, Sheep, Base Sequence, 030306 microbiology, génome, lcsh:R, Endogenous Retroviruses, Reproducibility of Results, Cancers and Neoplasms, biology.organism_classification, Molecular biology, In vitro, Viruses and Cancer, lcsh:Q, Mutant Proteins, Betaretrovirus
Abstract

International audience; The Jaagsiekte sheep retrovirus exJSRV and its endogenous counterpart enJSRV co-exist in sheep. exJSRV, a betaretrovirus, is the etiological agent of ovine pulmonary adenocarcinoma, and it has been demonstrated in vitro that an enJSRV Gag variant bearing the R-to-W amino acid change at position 21 was able to block exJSRV budding from the cells, providing a potential protective role for the host. In this work, we developed a fast mutation detection assay based on the oligo ligation assay (OLA) that permits the quantification of the relative proportions of the R21 and W21 Gag variants present in individual genomes and in cDNA obtained from normal and exJSRV-induced lung tumors. We have shown that the W21/R21 ratio is variable within and between breeds. We also describe for the first time that putative protecting enJSRV variants were expressed in alveolar type II cells (AECII), the major target of exJSRV.

Published
2012
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38. Neuroimmune connections in ovine pharyngeal tonsil: potential site for prion neuroinvasion

Author

Luc Grobet, Laurent Massart, Vinciane Toppets, Isabelle Lantier, Patricia Berthon, Joëlle Piret, Georges Daube, Nathalie Kirschvink, Frédéric Lantier, Nadine Antoine, Université de Liège, Université de Namur [Namur] (UNamur), Infectiologie et Santé Publique (UMR ISP), Institut National de la Recherche Agronomique (INRA)-Université de Tours, and Institut National de la Recherche Agronomique (INRA)-Université de Tours (UT)

Subjects
Pathology, medicine.medical_specialty, sheep, [SDV.OT]Life Sciences [q-bio]/Other [q-bio.OT], Aging, Histology, Genotype, Prions, Pharyngeal Tonsil, Scrapie, Biology, Nervous System, Pathology and Forensic Medicine, follicular dendritic cell, 03 medical and health sciences, 0302 clinical medicine, Nerve Fibers, pharyngeal tonsil, Neurofilament Proteins, Glial Fibrillary Acidic Protein, medicine, Animals, Sheep, Domestic, 030304 developmental biology, 0303 health sciences, Microscopy, Confocal, Follicular dendritic cells, Germinal center, Cell Biology, innervation, Germinal Center, Epithelium, medicine.anatomical_structure, Tonsil, Adenoids, Respiratory epithelium, Free nerve ending, 030217 neurology & neurosurgery, Cryoultramicrotomy, Dendritic Cells, Follicular
Abstract

International audience; Recent studies have established the involvement of nasal-associated lymphoid tissues, mainly the pharyngeal tonsil, in prion pathogenesis. However, the mechanisms of the associated neuroinvasion are still debated. To determine potential sites for prion neuroinvasion inside the ovine pharyngeal tonsil, the topography of heavy (200 kDa) and light (70 kDa) neurofilaments and of glial fibrillar acidic protein has been semi-quantitatively analysed inside the various compartments of the tonsil. The results show that the most innervated areas are the interfollicular area and the connective tissue located beneath the respiratory epithelium. The existence of rare synapses between follicular dendritic cells and nerve fibres inside the germinal centre indicates that this mechanism of neuroinvasion is possible but, since germinal centres of lymphoid follicles are poorly innervated, other routes of neuroinvasion are likely. The host PRNP genotype does not influence the pattern of innervation in these various tonsil compartments, unlike ageing during which an increase of nerve endings occurs in a zone of high trafficking cells beneath the respiratory epithelium. A minimal age-related increase of innervation inside the lymphoid follicles has also been observed. An increase in nerve fibre density around the lymphoid follicles, in an area rich in mobile cells such as macrophages and dendritic cells capable of capturing and conveying pathogen prion protein (PrPd), might ensure more efficient infectivity, not in the early phase but in the advanced phase of lymphoinvasion after the amplification of PrPd; alternatively, this area might even act as a direct site of entry during neuroinvasion.

Published
2011
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39. Ultra-early weaning in piglets results in low serum IgA concentration and IL17 mRNA expression

Author

Mustapha Berri, Claire Chevaleyre, François Meurens, Benoît Levast, Isabelle Lantier, Françoise Mangin, Michèle de Monte, Henri Salmon, Galliano Zanello, Sandrine Melo, Infectiologie Animale et Santé Publique (UR IASP), Institut National de la Recherche Agronomique (INRA), Université Francois Rabelais [Tours], and Conseil Regional du Centre (France)

Subjects
medicine.medical_specialty, TISSUES, Swine, medicine.medical_treatment, animal diseases, [SDV]Life Sciences [q-bio], Immunology, Weaning, Biology, Mesenteric lymph node, 03 medical and health sciences, fluids and secretions, 0302 clinical medicine, Immune system, Blood serum, Internal medicine, Gene expression, INFECTION, medicine, Animals, PROTECTION, RNA, Messenger, Cytokine, Barrier function, 030304 developmental biology, 0303 health sciences, SMALL-INTESTINE, PIG, CONSEQUENCES, General Veterinary, Gene Expression Profiling, Interleukin-17, FOXP3, DYSFUNCTION, Immunoglobulin A, Endocrinology, Piglets, Milk, CELLS, GROWTH, Swine, Miniature, Lymph, Lymph Nodes, IgA, 030215 immunology
Abstract

International audience; In pigs raised for meat production, weaning is a critical period because of related physiological perturbations and negative consequences on performance. Previous studies have shown that early weaning could either impair development of mucosal barrier function or boost intestinal immunologic parameters. In order to obtain further knowledge about the impact of ultra-early weaning on the porcine immune system development, three groups of piglets were weaned at different ages and compared to the unweaned control group. Lower IgA concentrations in ultra-early and early weaned piglets than in other piglets were identified in serum. In the mesenteric lymph node (MLN), significant differences in the mRNA expression of IL17a,TGF beta and FOXP3 were found between specific groups. Indeed, IL17a mRNA was mainly detected in ultra-early weaned piglets while FOXP3 and TGF beta mRNA were associated to both ultra-early weaned and suckling piglets. Reduced serum IgA concentration and MLN induction of a Th17 cytokine in ultra-early weaned piglets could be related to alterations of the mucosal barrier functions consecutive to the milk deprivation. All together, our findings suggest a crucial role for endogenous milk factors onto the onset of IgA synthesis. (c) 2010 Elsevier BM. All rights reserved.

Published
2010
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40. The bovine prion agent BSE is vertically and horizontally transmissible in sheep flocks

Author

Frédéric Lantier, Patricia Berthon, Isabelle Lantier, Céline Barc, Christelle Rossignol, Hervé Leroux, Philippe Bernardet, Simmons, H., Jm Torres, Grassi, J., Simon, S., Olivier Andréoletti, Infectiologie Animale et Santé Publique (UR IASP), Institut National de la Recherche Agronomique (INRA), Plateforme d'Infectiologie Expérimentale (PFIE), VLA Weybridge, Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria = National Institute for Agricultural and Food Research and Technology (INIA), Commissariat à l'énergie atomique et aux énergies alternatives (CEA), Interactions hôtes-agents pathogènes [Toulouse] (IHAP), Institut National de la Recherche Agronomique (INRA)-Ecole Nationale Vétérinaire de Toulouse (ENVT), Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées, ProdInra, Migration, Université de Toulouse (UT)-Université de Toulouse (UT)-Institut National Polytechnique (Toulouse) (Toulouse INP), and Université de Toulouse (UT)-Université de Toulouse (UT)

Subjects
[SDV] Life Sciences [q-bio], [SDV]Life Sciences [q-bio], ComputingMilieux_MISCELLANEOUS
Abstract

National audience

Published
2009

41. Co-infection par l'ESB et la tremblante inoculées par voie intracérébrale chez les ovins : la tremblante masque l'ESB dans le système nerveux central mais pas dans les tissus lymphoïdes

Author

Frédéric Lantier, Patricia Berthon, Isabelle Lantier, Céline Barc, Hervé Le Roux, Christelle Rossignol, Philippe Bernardet, Caroline Lacroux, Simmons, H., Grassi, J., Jm Torres, Simon, S., Olivier Andréoletti, Infectiologie Animale et Santé Publique (UR IASP), Institut National de la Recherche Agronomique (INRA), Plateforme d'Infectiologie Expérimentale (PFIE), Interactions hôtes-agents pathogènes [Toulouse] (IHAP), Institut National de la Recherche Agronomique (INRA)-Ecole Nationale Vétérinaire de Toulouse (ENVT), Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées, VLA Weybridge, Commissariat à l'énergie atomique et aux énergies alternatives (CEA), Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria = National Institute for Agricultural and Food Research and Technology (INIA), ProdInra, Migration, Université de Toulouse (UT)-Université de Toulouse (UT)-Institut National Polytechnique (Toulouse) (Toulouse INP), and Université de Toulouse (UT)-Université de Toulouse (UT)

Subjects
[SDV] Life Sciences [q-bio], [SDV]Life Sciences [q-bio], ComputingMilieux_MISCELLANEOUS
Abstract

National audience

Published
2009

42. Protective effect of the T112 PrP variant in sheep challenged with bovine spongiform encephalopathy

Author

Ginny C. Saunders, Olivier Andreoletti, S. J. Moore, S. Bellworthy, Otto Windl, Mark Arnold, Isabelle Lantier, Frédéric Lantier, Patricia Berthon, Marion Simmons, Saira Cawthraw, Inconnu, Infectiologie Animale et Santé Publique (UR IASP), Institut National de la Recherche Agronomique (INRA), Interactions hôtes-agents pathogènes [Toulouse] (IHAP), Institut National de la Recherche Agronomique (INRA)-Ecole Nationale Vétérinaire de Toulouse (ENVT), Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National Polytechnique (Toulouse) (Toulouse INP), and Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées

Subjects
Genotype, Prions, 040301 veterinary sciences, animal diseases, Bovine spongiform encephalopathy, [SDV]Life Sciences [q-bio], biology.animal_breed, Sheep Diseases, Biology, Asymptomatic, PRNP, 0403 veterinary science, 03 medical and health sciences, Virology, mental disorders, medicine, Animals, Genetic Predisposition to Disease, Allele, ComputingMilieux_MISCELLANEOUS, 030304 developmental biology, 0303 health sciences, Sheep, food and beverages, 04 agricultural and veterinary sciences, medicine.disease, nervous system diseases, 3. Good health, Encephalopathy, Bovine Spongiform, Prnp gene, Suffolk sheep, Cattle, medicine.symptom, Prion Proteins
Abstract

Sheep with an ARQ/ARQ PRNP genotype at codon positions 136/154/171 are highly susceptible to experimental infection with bovine spongiform encephalopathy (BSE). However, a number of sheep challenged orally or intracerebrally with BSE were clinically asymptomatic and found to survive or were diagnosed as BSE-negative when culled. Sequencing of the full PRNP gene open reading frame of BSE-susceptible and -resistant sheep indicated that, in the majority of Suffolk sheep, resistance was associated with an M112T PRNP variant (TARQ allele). A high proportion (47 of 49; 96 %) of BSE-challenged wild-type (MARQ/MARQ) Suffolk sheep were BSE-infected, whereas none of the 20 sheep with at least one TARQ allele succumbed to BSE. Thirteen TARQ-carrying sheep challenged with BSE are still alive and some have survival periods equivalent to, or greater than, reported incubation periods of BSE in ARR/ARR and VRQ/VRQ sheep.

Published
2009
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43. Transgenic mice expressing porcine prion protein resistant to classical scrapie but susceptible to sheep bovine spongiform encephalopathy and atypical scrapie

Author

Danielle Padilla, Juan María Torres, Joaquín Castilla, Maria Eugenia Herva, Isabelle Lantier, Hervé Cassard, Olivier Andreoletti, Caroline Lacroux, Juan Carlos Espinosa, Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria = National Institute for Agricultural and Food Research and Technology (INIA), Interactions hôtes-agents pathogènes [Toulouse] (IHAP), Institut National de la Recherche Agronomique (INRA)-Ecole Nationale Vétérinaire de Toulouse (ENVT), Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées, Infectiologie Animale et Santé Publique (UR IASP), and Institut National de la Recherche Agronomique (INRA)

Subjects
Epidemiology, Swine, [SDV]Life Sciences [q-bio], animal diseases, lcsh:Medicine, Scrapie, Communicable Diseases, Emerging, law.invention, 0403 veterinary science, Mice, law, Porcine prion, Genotype, 0303 health sciences, Strain (chemistry), Brain, 04 agricultural and veterinary sciences, Classical scrapie, Phenotype, Recombinant Proteins, 3. Good health, Encephalopathy, Bovine Spongiform, prions and related diseases, Infectious Diseases, Recombinant DNA, transmission barrier, Microbiology (medical), Genetically modified mouse, atypical scrapie, sheep, 040301 veterinary sciences, Prions, Bovine spongiform encephalopathy, Sheep Diseases, Mice, Transgenic, Biology, lcsh:Infectious and parasitic diseases, 03 medical and health sciences, Species Specificity, medicine, Animals, lcsh:RC109-216, bovine spongiform encephalopathy, 030304 developmental biology, Brain Chemistry, Research, scrapie, lcsh:R, medicine.disease, Virology, nervous system diseases, Disease Models, Animal, Cattle
Abstract

Atypical scrapie strain phenotypes may shift when transmitted to a new host., How susceptible pigs are to infection with sheep prions is unknown. We show, through transmission experiments in transgenic mice expressing porcine prion protein (PrP), that the susceptibility of this mouse model to bovine spongiform encephalopathy (BSE) can be enhanced after its passage in ARQ sheep, indicating that the pathogenicity of the BSE agent is modified after passage in sheep. Transgenic mice expressing porcine PrP were, nevertheless, completely resistant to infection with a broad panel of classical scrapie isolates from different sheep PrP genotypes and with different biochemical characteristics. The atypical (Nor98 like) isolate (SC-PS152) was the only scrapie isolate capable of transmission in these mice, although with a marked transmission barrier. Unexpectedly, the atypical scrapie agent appeared to undergo a strain phenotype shift upon transmission to porcine-PrP transgenic mice and acquired new strain properties, suggesting that atypical scrapie agent may exhibit different phenotypes depending on the host cellular PrP or other genetic factors.

Published
2009
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44. Application de l'oxydation par voie humide à la destruction du prion de l'ESB

Author

Zalouk, S., Barbati, S., Isabelle Lantier, Leroux, H., Frederic Lantier, Infectiologie Animale et Santé Publique (UR IASP), Institut National de la Recherche Agronomique (INRA), Laboratoire Chimie Provence (LCP), Centre National de la Recherche Scientifique (CNRS)-Université de Provence - Aix-Marseille 1-Institut de Chimie du CNRS (INC), Inconnu, Université de Provence - Aix-Marseille 1-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), and ProdInra, Migration

Subjects
[SDV] Life Sciences [q-bio], [SDV]Life Sciences [q-bio], ComputingMilieux_MISCELLANEOUS
Abstract

National audience

Published
2009

45. Induction of lactogenic immunity to transmissible gastroneteritis virus of swine using an attenuated coronavirus mutant able to survive in the physicochemical environment of the digestive tract

Author

Elisabeth Bottreau, Henri Salmon, Ph. Vannier, Isabelle Lantier, J.M. Aynaud, and S. Bernard

Subjects
Swine, animal diseases, Immunization, Secondary, Administration, Oral, Biology, medicine.disease_cause, Antibodies, Viral, Vaccines, Attenuated, Injections, Intramuscular, Microbiology, Article, Immune system, fluids and secretions, Immunity, Lactation, medicine, Animals, Neutralizing antibody, Coronavirus, General Veterinary, Gastroenteritis, Transmissible, of Swine, Transmissible gastroenteritis virus, Vaccination, food and beverages, Viral Vaccines, General Medicine, Virology, Animals, Suckling, Titer, medicine.anatomical_structure, Milk, Humoral immunity, biology.protein, Female, Immunity, Maternally-Acquired
Abstract

A transmissible gastroenteritis (TGE) coronavirus mutant (188-SG), selected as attenuated and resistant to acidity and proteases of the digestive tract of adult pigs, was used as vaccine ("Nouzilly strain") in sows to protect suckling piglets against a challenge exposure carried out with a highly virulent TGEV strain. The pregnant sows were immunized once (42-49 days before farrowing) or twice (42-49 and 7-15 days before farrowing) by the oral, intramuscular or conjunctival route with the 188-SG strain. Sows exposed to virulent TGEV in the field and experimentally infected sows (two oral inoculations during pregnancy) were used as positive controls leading to high protection. The neutralizing antibody response to vaccination and/or infection was studied in serum and milk. No protection against mortality was observed in the litters of (1) the nine seronegative, susceptible sows, with piglet mortality of 65/70, (2) the seven once orally vaccinated sows, with mortality of 44/54, (3) the seven sows vaccinated twice by the conjunctival route, with mortality of 55/76. Moderate protection was observed in (1) the eight sows vaccinated intramuscularly twice with piglet mortality of 36/90, (2) the seven orally and intramuscularly vaccinated sows with piglet mortality of 31/51. In of 3 contrast, improved protection was observed in (1) the 10 sows vaccinated twice orally, with piglet mortality of 23/95, (2) the four naturally infected sows with piglet mortality of 6/41, (3) the six sows experimentally infected with virulent TGEV with piglet mortality of 1/59. No correlation was found between neutralizing antibodies titers in serum and milk and protection rate of the piglets. The results indicate that relative protective lactogenic immunity against TGEV is induced only by repeated ingestion of the attenuated 188-SG strain of TGEV.

Published
1991
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46. Intracerebrally-induced BSE in goats

Author

Patricia Berthon, Isabelle Lantier, Céline Barc, Christelle Rossignol, Leroux, H., Philippe Bernardet, Grassi, J., Olivier Andréoletti, Frédéric Lantier, Infectiologie Animale et Santé Publique (UR IASP), Institut National de la Recherche Agronomique (INRA), Plateforme d'Infectiologie Expérimentale (PFIE), Commissariat à l'énergie atomique et aux énergies alternatives (CEA), Interactions hôtes-agents pathogènes [Toulouse] (IHAP), Institut National de la Recherche Agronomique (INRA)-Ecole Nationale Vétérinaire de Toulouse (ENVT), Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées, Université de Toulouse (UT)-Université de Toulouse (UT)-Institut National Polytechnique (Toulouse) (Toulouse INP), Université de Toulouse (UT)-Université de Toulouse (UT), and ProdInra, Migration

Subjects
[SDV] Life Sciences [q-bio], [SDV]Life Sciences [q-bio], ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
2008

47. BSE transmission to lambs born from infected or contact ewes

Author

Frédéric Lantier, Patricia Berthon, Isabelle Lantier, Céline Barc, Christelle Rossignol, Leroux, H., Philippe Bernardet, Simmons, H., Grassi, J., Simon, S., Olivier Andréoletti, Infectiologie Animale et Santé Publique (UR IASP), Institut National de la Recherche Agronomique (INRA), Plateforme d'Infectiologie Expérimentale (PFIE), Veterinary Laboratories Agency, Commissariat à l'énergie atomique et aux énergies alternatives (CEA), Interactions hôtes-agents pathogènes [Toulouse] (IHAP), Institut National de la Recherche Agronomique (INRA)-Ecole Nationale Vétérinaire de Toulouse (ENVT), Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées, ProdInra, Migration, Université de Toulouse (UT)-Université de Toulouse (UT)-Institut National Polytechnique (Toulouse) (Toulouse INP), and Université de Toulouse (UT)-Université de Toulouse (UT)

Subjects
[SDV] Life Sciences [q-bio], [SDV]Life Sciences [q-bio], ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
2008

48. Oral transmission of BSE in resistant ARR/ARR sheep: involvement of lymphoid tissues

Author

Frédéric Lantier, Patricia Berthon, Isabelle Lantier, Céline Barc, Christelle Rossignol, Hugues Leroux, Jacques Grassi, Simmons, H., Jm Torres, Olivier Andréoletti, ProdInra, Migration, Infectiologie Animale et Santé Publique (UR IASP), Institut National de la Recherche Agronomique (INRA), Inconnu, Interactions hôtes-agents pathogènes [Toulouse] (IHAP), Institut National de la Recherche Agronomique (INRA)-Ecole Nationale Vétérinaire de Toulouse (ENVT), Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National Polytechnique (Toulouse) (Toulouse INP), and Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées

Subjects
[SDV] Life Sciences [q-bio], [SDV]Life Sciences [q-bio], ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
2008

49. Lactogenic Immunity to Transmissible Gastroenteritis (TGE) of swine induced by the attenuated nouzilly strain of TGE virus: Passive protection of piglets and detection of serum and milk antibody classes by ELISA

Author

J.M. Aynaud, Serge Bernard, Isabelle Lantier, June Shirai, Elisabeth Bottreau, ProdInra, Migration, Laboratoire de pathologie porcine (et immunologie) [Nouzilly] (Unité 702), and Institut National de la Recherche Agronomique (INRA)

Subjects
Coronaviridae, Swine, 040301 veterinary sciences, [SDV]Life Sciences [q-bio], animal diseases, Immunology, Immunoglobulins, Virulence, Enzyme-Linked Immunosorbent Assay, Biology, Antibodies, Viral, Vaccines, Attenuated, medicine.disease_cause, Article, Virus, Microbiology, 0403 veterinary science, 03 medical and health sciences, Neutralization Tests, Pregnancy, Immunity, medicine, Animals, Antibody Classes, Cells, Cultured, ComputingMilieux_MISCELLANEOUS, 030304 developmental biology, Coronavirus, 0303 health sciences, General Veterinary, Strain (chemistry), Gastroenteritis, Transmissible, of Swine, Transmissible gastroenteritis virus, Immunization, Passive, Viral Vaccines, 04 agricultural and veterinary sciences, Virology, 3. Good health, [SDV] Life Sciences [q-bio], Milk, Immunization, biology.protein, Female, Antibody
Abstract

Piglets of eight sows vaccinated by different routes with the attenuated TGE mutant coronavirus, Nouzilly (N) strain, and piglets from two field seropositive sows were challenged with a virulent TGE strain. On the day of challenge and 10 days after challenge, milk and serum samples from sows were analysed for their level of neutralizing antibodies, total immunoglobulin classes and TGE antibody classes by an ELISA. No direct relationship was seen between the level of protection of the litters and the titres of the different antibody classes on the day of challenge. However, an inverse correlation was seen 10 days after challenge between protection and the level of TGE antibodies.

Published
1990
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50. Genetics of susceptiblility to transmissible spongiform encephalopathies in sheep

Author

Frédéric Lantier, Patricia Berthon, Isabelle Lantier, Céline Barc, Christelle Rossignol, Philippe Bernardet, Pierre Sarradin, Leroux, H., Serge Bernard, Caroline Lacroux, Grassi, J., Ha Simmons, François Shelcher, Jean Michel Elsen, Olivier Andréoletti, ProdInra, Migration, Infectiologie Animale et Santé Publique (UR IASP), Institut National de la Recherche Agronomique (INRA), Plateforme d'Infectiologie Expérimentale (PFIE), Interactions hôtes-agents pathogènes [Toulouse] (IHAP), Institut National de la Recherche Agronomique (INRA)-Ecole Nationale Vétérinaire de Toulouse (ENVT), Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées, Commissariat à l'énergie atomique et aux énergies alternatives (CEA), VLA, Station d'Amélioration Génétique des Animaux (SAGA), Université de Toulouse (UT)-Université de Toulouse (UT)-Institut National Polytechnique (Toulouse) (Toulouse INP), and Université de Toulouse (UT)-Université de Toulouse (UT)

Subjects
[SDV] Life Sciences [q-bio], [SDV]Life Sciences [q-bio], ComputingMilieux_MISCELLANEOUS
Abstract

National audience

Published
2007

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