14 results on '"Isabel Guerra-Merino"'
Search Results
2. In silico identification and in vitro expression analysis of breast cancer-related m6A-SNPs
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Tamara Kleinbielen, Felix Olasagasti, Daniel Azcarate, Elena Beristain, Amparo Viguri-Díaz, Isabel Guerra-Merino, África García-Orad, and Marian M. de Pancorbo
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breast cancer ,epigenetics ,m6a methylation ,m6a-snp ,snp ,Genetics ,QH426-470 - Abstract
Research on m6A-associated SNPs (m6A-SNPs) has emerged recently due to their possible critical roles in many key biological processes. In this sense, several investigations have identified m6A-SNPs in different diseases. In order to gain a more complete understanding of the role that m6A-SNPs can play in breast cancer, we performed an in silico analysis to identify the m6A-SNPs associated with breast cancer and to evaluate their possible effects. For this purpose, we downloaded SNPs related to breast cancer and a list of m6A-SNPs from public databases in order to identify which ones appear in both. Subsequently, we assessed the identified m6A-SNPs in silico by expression quantitative trait loci (eQTL) analysis and differential gene expression analysis. We genotyped the m6A-SNPs found in the in silico analysis in 35 patients with breast cancer, and we carried out a gene expression analysis experimentally on those that showed differences. Our results identified 981 m6A-SNPs related to breast cancer. Four m6A-SNPs showed an eQTL effect and only three were in genes that presented an altered gene expression. When the three m6A-SNPs were evaluated in the tissue sample of our breast cancer patients, only the m6A-SNP rs76563149 located in ZNF354A gene presented differences in allele frequencies and a low gene expression in breast cancer tissues, especially in luminal B HER2+ subtype. Future investigations of these m6A-SNPs should expand the study in different ethnic groups and increase the sample sizes to test their association with breast cancer and elucidate their molecular function.
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- 2022
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3. m6A levels and expression of its modification genes show significant differences in breast cancer molecular subtypes
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Tamara Kleinbielen, Felix Olasagasti, Elena Beristain, Amparo Viguri-Díaz, Isabel Guerra-Merino, Africa García-Orad, and Marian M. de Pancorbo
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Cell Biology ,Molecular Biology ,Biochemistry ,Genetics (clinical) - Published
- 2022
4. Miki (Mitotic Kinetics Regulator) Immunoexpression in Normal Liver, Cirrhotic Areas and Hepatocellular Carcinomas: a Preliminary Study with Clinical Relevance
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Beatriz García, Isabel Guerra-Merino, Francisco Borja Gutiérrez-Corres, José Javier Aguirre, Jorge Santos-Juanes, Luis M. Quirós, Laura Lorente-Gea, Iván Fernández-Vega, and Emma Camacho-Urkaray
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Liver Cirrhosis ,Male ,0301 basic medicine ,Cytoplasm ,Cancer Research ,Pathology ,medicine.medical_specialty ,Carcinoma, Hepatocellular ,Proliferative index ,Cell Cycle Proteins ,Kaplan-Meier Estimate ,Biology ,Pathology and Forensic Medicine ,03 medical and health sciences ,0302 clinical medicine ,medicine ,Humans ,Clinical significance ,Aged ,Aged, 80 and over ,Cell Nucleus ,Tissue microarray ,Liver Neoplasms ,General Medicine ,Middle Aged ,HCCS ,medicine.disease ,Immunohistochemistry ,digestive system diseases ,Staining ,Ki-67 Antigen ,030104 developmental biology ,Liver ,Oncology ,030220 oncology & carcinogenesis ,Hepatocellular carcinoma ,Cancer cell ,Female - Abstract
Hepatocellular carcinoma (HCC) is the most common type of primary malignant tumor in the liver. One of the main features of cancer survival is the generalized loss of growth control exhibited by cancer cells, and Miki is a protein related to the immunoglobulin superfamily that plays an important role in mitosis. We aim to study protein expression levels of Miki in non-tumoral liver and 20 HCCs recruited from a Pathology Department. Clinical information was also obtained. A tissue microarray was performed, and immunohistochemical techniques applied to study protein expression levels of Miki. In normal liver, Miki was weakly expressed, showing nuclear staining in the hepatocytes. Cirrhotic areas and HCCs showed a variety of staining patterns. Most HCC samples showed positive expression, with three different staining patterns being discernible: nuclear, cytoplasmic and mixed. Statistical analysis showed a significant association between grade of differentiation, Ki-67 proliferative index, survival rates and staining patterns. This study has revealed the positive expression of Miki in normal liver, cirrhotic areas and HCCs. Three different staining patterns of Miki expression with clinical relevance were noted in HCCs.
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- 2018
5. Establishing cut-off points with clinical relevance for bcl-2, cyclin D1, p16, p21, p27, p53, Sox11 and WT1 expression in glioblastoma - a short report
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Beatriz García, Iván Fernández-Vega, Jorge Santos-Juanes, Emma Camacho-Urkaray, José Javier Aguirre, Isabel Guerra-Merino, Francisco Borja Gutiérrez-Corres, and Luis M. Quirós
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Cyclin-Dependent Kinase Inhibitor p21 ,Male ,Oncology ,Cancer Research ,medicine.medical_specialty ,Protein biomarkers ,SOXC Transcription Factors ,03 medical and health sciences ,0302 clinical medicine ,Cyclin D1 ,Internal medicine ,medicine ,Humans ,Diagnostic biomarker ,Clinical significance ,WT1 Proteins ,Cyclin-Dependent Kinase Inhibitor p16 ,Survival analysis ,business.industry ,General Medicine ,Middle Aged ,medicine.disease ,Immunohistochemistry ,Tissue Array Analysis ,030220 oncology & carcinogenesis ,Molecular Medicine ,Biomarker (medicine) ,Female ,Tumor Suppressor Protein p53 ,Glioblastoma ,business ,030217 neurology & neurosurgery - Abstract
Glioblastoma (GBM) ranks among the most challenging cancers to treat and there is an urgent need for clinically relevant prognostic and diagnostic biomarkers. Here, we set out to investigate the expression of eight proteins (bcl-2, cyclin D1, p16, p21, p27, p53, Sox11 and WT1) in GBM with the specific aim to establish immunohistochemistry cut-off points with clinical relevance.Immunohistochemistry (IHC) was used to examine protein expression in 55 surgical GBM specimens using H-scores, and IHC cut-off points were established using the Cutoff Finder web platform. Protein co-expression and its correlation with histopathological features were assessed, and cases were classified according to IDH1 mutation status. Survival curves were determined using Kaplan-Meier analyses.Clinical and molecular parameters found to be correlated with overall survival (OS) were tumor size (r = -0.278; p = 0.048), p53 (r = -0.452; p = 0.001), p16 (r = 0.351; p = 0.012) and Sox11 (r = 0.324; p = 0.020). In addition, we found that tumor size correlated with cyclin D1 (r = -0.282; p = 0.037), p53 (r = 0.269; p = 0.041), Sox11 (r = -0.309; p = 0.022) and WT1 (r = -0.372; p = 0.003). Variables found to be significantly associated with IDH1 mutation status were OS (p 0.01), age (p 0.01), cyclin D1 (p = 0.046), p16 (p = 0.019) and Sox11 (p = 0.012). Variables found to be significantly associated with a poor survival were tumor size5 cm (p 0.001), bcl-2 score 40 (p = 0.034), cyclin D1 score ≤ 70 (p = 0.004), p16 score ≤ 130 (p = 0.005), p53 score 20 (p = 0.003), Sox11 score ≤ 40 (p 0.001) and WT1 score ≤ 270 (p = 0.02).Correlations between protein biomarkers and main clinical GBM variables were identified. The establishment of distinct biomarker cut-off points may enable clinicians and pathologists to better weigh their prognostic value.
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- 2017
6. Prognostic and therapeutic value of somatic mutations in diffuse large B-cell lymphoma: A systematic review
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Maria Lopez-Santillan, Idoia Martin-Guerrero, Garazi Martinez, Angela Gutierrez-Camino, Isabel Guerra-Merino, Elixabet Lopez-Lopez, Irune Ruiz-Diaz, Javier Arzuaga-Mendez, and Paula Alvarez-Gonzalez
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Oncology ,rituximab-chop ,medicine.medical_specialty ,Somatic cell ,CD58 ,medicine.medical_treatment ,DNA Mutational Analysis ,diffuse large B-cell lymphoma ,Targeted therapy ,Germline mutation ,Refractory ,immune system diseases ,hherapeutic target ,hemic and lymphatic diseases ,Internal medicine ,medicine ,Humans ,P53 gene-mutationspoor survival ,somatic mutation ,TP53 ,EZH2 ,predict ,business.industry ,Hematology ,Prognosis ,tumor-suppressor ,medicine.disease ,disruption ,Lymphoma ,Clinical trial ,classification ,Mutation ,outcome ,Lymphoma, Large B-Cell, Diffuse ,methylation ,Neoplasm Recurrence, Local ,business ,Diffuse large B-cell lymphoma - Abstract
Diffuse large B-cell lymphoma (DLBCL), the most common type of Non-Hodgkin lymphoma (NHL), is a highly heterogeneous and aggressive disease. Regardless of this heterogeneity, all patients receive the same first-line therapy, which fails in 30-40 % of patients, who are either refractory or relapse after remission. With the aim of stratifying patients to improve treatment outcome, different clinical and genetic biomarkers have been studied. The present systematic review aimed to identify somatic mutations that could serve as prognosis biomarkers or as therapeutic target mutations in DLBCL. Regarding their role as prognostic markers, mutations in CD58 and TP53 seem the most promising predictors of poor outcome although the combination of different alterations and other prognostic factors could be a more powerful strategy. On the other hand, different approaches regarding targeted therapy have been proposed. Therefore, mutational analysis could help guide treatment choice in DLBCL yet further studies and clinical trials are needed. This study was funded by the Basque Government (IT989-16) and EiTB maratoia/Bioef (BIO15/CA/022/BC) . AGC was supported by a post-doctoral grant from the Basque Government.
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- 2021
7. Upregulated Expression of Heparanase and Heparanase 2 in the Brains of Alzheimer’s Disease
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Carla Martín, Jesus Merayo-Lloves, Iván Fernández-Vega, Luis M. Quirós, Laura Lorente-Gea, Irune Ruiz-Diaz, Helena Ordiales, Beatriz García, María Cristina Caballero-Martínez, Isabel Guerra-Merino, Kelvin Piña Batista, Jorge Santos-Juanes, Ikerne Vicente-Etxenausia, Sonia Castañón, Bárbara Muñiz-Alonso, Olivia García-Suárez, and Santiago Fernández-Menéndez
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Male ,0301 basic medicine ,Biology ,Glycosaminoglycan ,03 medical and health sciences ,chemistry.chemical_compound ,Downregulation and upregulation ,Alzheimer Disease ,Extracellular ,Humans ,Heparanase ,RNA, Messenger ,Senile plaques ,Aged ,Glucuronidase ,Aged, 80 and over ,General Neuroscience ,Brain ,General Medicine ,Heparan sulfate ,Middle Aged ,Up-Regulation ,Cell biology ,Psychiatry and Mental health ,Clinical Psychology ,030104 developmental biology ,chemistry ,Immunohistochemistry ,Female ,Geriatrics and Gerontology ,Intracellular - Abstract
BACKGROUND Heparan sulfate proteoglycans (HSPGs) promote amyloid-β peptide and tau fibrillization in Alzheimer's disease (AD) and provide resistance against proteolytic breakdown. Heparanase (HPSE) is the only enzyme that cleaves heparan sulfate (HS). Heparanase 2 (HPSE2) lacks HS-degrading activity, although it is able to interact with HS with high affinity. OBJECTIVE To analyze HPSE and HPSE2 expressions at different stages of AD. METHODS RT-PCR was used to analyze transcription levels of both heparanases at different stages of AD, and immunohistochemistry was performed to localize each one in different parts of the brain. RESULTS Both proteins appeared overexpressed at different stages of AD. Immunohistochemistry indicated that the presence of the heparanases was related to AD pathology, with intracellular deposits found in degenerated neurons. At the extracellular level, HPSE was observed only in neuritic plaques with a fragmented core, while HPSE2 appeared in those with compact cores as well. CONCLUSION Given the involvement of HSPGs in AD pathology, there would seem to be a relationship between the regulation of heparanase expression, the features of the disease, and a possible therapeutic alternative.
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- 2017
8. Expression of the DYRK1A gene correlates with its 3D positioning in the interphase nucleus of Down syndrome cells
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José L. Zugaza, Nerea Paz, Izaskun Felipe-Blanco, Isabel Guerra-Merino, Africa Garcia-Orad, Amaia Zabala, Felix Royo, and Luis Antonio Parada
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Down syndrome ,DYRK1A ,Chromosomes, Human, Pair 21 ,Gene Expression ,Protein Serine-Threonine Kinases ,Biology ,Cell Line ,Chromosome 15 ,Superoxide Dismutase-1 ,Genetics ,medicine ,Humans ,Interphase ,Gene ,Alleles ,In Situ Hybridization, Fluorescence ,Cell Nucleus ,Superoxide Dismutase ,Chromosome ,Protein-Tyrosine Kinases ,medicine.disease ,Genetic Loci ,Down Syndrome ,Trisomy ,Chromosome 21 - Abstract
Down syndrome is a common birth defect caused by trisomy of chromosome 21. Chromosomes occupy distinct territories in interphase nuclei, and their distribution within the nuclear space is nonrandom. In humans with Down syndrome, two chromosomes 21 frequently localize proximal to one another and distant from the third chromosome. Here, we investigated the nuclear organization of DYRK1A and SOD1, two genes mapping to chromosome 21 that greatly contribute to the pathology. We found that DYRK1A conserves its central positioning between normal and trisomic cells, whereas SOD1 adopts more peripheral distribution in trisomic cells. We also found that the relative position of these genes with respect to each other varies among the different copies of chromosome territories 21 within a cell, and that this distinct distribution is associated with differences in their expression levels. All together, our results may explain, at least in part, the difference in the expression level of these two genes implicated in the pathogenesis of Down syndrome.
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- 2015
9. Lack of association of the CEP72 rs924607 TT genotype with vincristine-related peripheral neuropathy during the early phase of pediatric acute lymphoblastic leukemia treatment in a Spanish population
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Elixabet Lopez-Lopez, Angela Gutierrez-Camino, Aizpea Echebarria-Barona, Isabel Guerra-Merino, Iñaki Zabalza, Idoia Martin-Guerrero, Irune Ruiz, and Africa Garcia-Orad
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0301 basic medicine ,Oncology ,Vincristine ,medicine.medical_specialty ,Genotype ,Population ,03 medical and health sciences ,0302 clinical medicine ,Internal medicine ,Genetics ,medicine ,Humans ,Dosing ,General Pharmacology, Toxicology and Pharmaceutics ,Child ,education ,Molecular Biology ,Genetics (clinical) ,education.field_of_study ,business.industry ,Neurotoxicity ,Peripheral Nervous System Diseases ,Precursor Cell Lymphoblastic Leukemia-Lymphoma ,medicine.disease ,030104 developmental biology ,Peripheral neuropathy ,Spain ,030220 oncology & carcinogenesis ,Toxicity ,Etiology ,Molecular Medicine ,business ,Microtubule-Associated Proteins ,medicine.drug - Abstract
Vincristine is a component of acute lymphoblastic leukemia (ALL) treatment with the potential to induce peripheral neuropathy. Recently, the CEP72 rs924607 TT genotype was found to be associated with vincristine-induced toxicity during the continuation phase in pediatric ALL patients treated on the Total XIIIB and COG AALL0433 protocols at St Jude Children's Research Hospital and Children's Oncology Group. This finding could provide a base for safer dosing of vincristine. Nevertheless, there are variations in vincristine regimens among ALL treatment protocols and phases in different populations. Therefore, the aim of this study was to determine whether the CEP72 rs924607 TT genotype is a useful marker of vincristine neuropathy during induction therapy among Spanish children with B-ALL treated on the LAL-SHOP protocols. No association was found between neurotoxicity during the induction phase and the rs924607 TT genotype. This lack of association could be because of population differences and/or differences in neurotoxicity etiology between induction and continuation phases of treatment.
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- 2016
10. Brains with sporadic Creutzfeldt-Jakob disease and copathology showed a prolonged end-stage of disease
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Isabel Guerra-Merino, Aitzol Miguelez-Rodriguez, Ikerne Vicente-Etxenausia, Katty Perez de Heredia-Goñi, Jorge Santos-Juanes, Luis M. Quirós, Laura Lorente-Gea, José Javier Aguirre, Beatriz García, and Iván Fernández-Vega
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0301 basic medicine ,Male ,Pathology ,medicine.medical_specialty ,Amyloid beta ,Biopsy ,Nerve Tissue Proteins ,tau Proteins ,Neuropathology ,Disease ,Kaplan-Meier Estimate ,Creutzfeldt-Jakob Syndrome ,Pathology and Forensic Medicine ,03 medical and health sciences ,0302 clinical medicine ,mental disorders ,medicine ,Humans ,Stage (cooking) ,Phosphorylation ,Survival analysis ,Aged ,Biological Specimen Banks ,Retrospective Studies ,Univariate analysis ,Amyloid beta-Peptides ,biology ,business.industry ,Neurodegeneration ,Brain ,General Medicine ,Middle Aged ,medicine.disease ,Immunohistochemistry ,nervous system diseases ,DNA-Binding Proteins ,030104 developmental biology ,Spain ,biology.protein ,Disease Progression ,alpha-Synuclein ,Dementia ,Female ,Autopsy ,business ,030217 neurology & neurosurgery - Abstract
AimsTo investigate the expression of major proteins related to primary neurodegenerative diseases and their prognostic significance in brains with Creutzfeldt-Jakob disease (CJD).Materials and methodsThirty consecutive cases of confirmed CJD during the period 2010–2015 at Basque Brain bank were retrospectively reviewed. Moreover, major neurodegenerative-associated proteins (phosphorylated Tau, 4R tau, 3R tau, alpha-synuclein, TDP43, amyloid beta) were tested. Clinical data were reviewed. Cases were divided according to the presence or absence of copathology. Survival curves were also determined.ResultsCopathology was significantly associated with survival in brains with CJD (4.2±1.2 vs 9.2±1.9; P=0.019) and in brains with MM1/MV1 CJD (2.1±1.0 vs 6.7±2.8; P=0.012). Besides, the presence of more than one major neurodegenerative-associated protein was significantly associated with survival (4.2±1.2 vs 10.7±2.6; P=0.017). Thus, univariate analyses further pointed out variables significantly associated with better survival: copathology in CJD (HR=0.430; P=0.033); more than one neurodegenerative-associated protein in CJD (HR=0.369; P=0.036) and copathology in MM1/MV1 CJD (HR=0.525; P=0.032).ConclusionThe existence of copathology significantly prolongs survival in patients with rapidly progressive dementia due to CJD. The study of major neurodegenerative-associated proteins in brains with CJD could allow us to further understand the molecular mechanisms behind prion diseases.
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- 2017
11. Coexistence of mixed phenotype Creutzfeldt-Jakob disease, Lewy body disease and argyrophilic grain disease plus histological features of possible Alzheimer's disease: A multi-protein disorder in an autopsy case
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Iván Fernández-Vega, Javier Ruiz-Ojeda, Ikerne Vicente-Etxenausia, María V. Geijo, Ramón A. Juste, Jose Luis Sánchez Menoyo, Isabel Guerra-Merino, Juan J. Zarranz, and Jennifer Mediavilla-García
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Primitive reflexes ,Pathology ,medicine.medical_specialty ,Cerebellum ,business.industry ,Neurodegeneration ,General Medicine ,medicine.disease ,Pathology and Forensic Medicine ,White matter ,Limbic system ,medicine.anatomical_structure ,Basal ganglia ,medicine ,Dementia ,Neurology (clinical) ,medicine.symptom ,business ,Myoclonus - Abstract
We report hereby an autopsy case of sporadic mixed phenotype CJD without hereditary burden and a long-term clinical course. An 80-year old man was diagnosed with mild cognitive impairment 27 months before death, caused by bronchopneumonia and severe respiratory impairment. During this time, the patient developed gradual mental deterioration, some sleeping problems and myoclonus. Other clinical manifestations were progressive gait problems, language deterioration, presence of primitive reflexes and irritability. In keeping with those symptoms, a rapidly evolving dementia was clinically suspected. Cerebrospinal fluid test for 14-3-3 protein was negative. However, an abnormal EEG and MRI at end-stage of disease were finally consistent with CJD. Post-mortem examination revealed a massive cortical neuronal loss with associated reactive astrocytosis, also evident in the white matter. Diffuse spongiform changes involving some basal ganglia, especially medial thalamus, some troncoencephalic nuclei, mainly inferior olivary nucleus and the molecular layer of the cerebellum were seen. Immunorreactive deposits for anti-prion protein antibody were present at different areas of the CNS. Additionally, Lewy bodies were observed at the brainstem and amygdala. Furthermore, argirophilic grains together with oligodendroglial coiled bodies and pre-tangle inclusions in the neurons from the limbic system containing hyperphosphorylated 4R tau were noted. To the best of our knowledge, this is the first case of CJD combined with Lewy body disease and argirophilic grain disease. Furthermore, we believe this case is an extremely rare combination of MM2-cortical-type and MM2-thalamic-type sporadic CJD (sCJD), which explains the broad spectrum of MM2-type sCJD findings and symptoms. Moreover, histological features of possible Alzheimer's disease were also reported.
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- 2014
12. Vincristine pharmacokinetics pathway and neurotoxicity during early phases of treatment in pediatric acute lymphoblastic leukemia
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Purificación García-Miguel, Angela Gutierrez-Camino, Aizpea Echebarria-Barona, Aurora Navajas, Isabel Guerra-Merino, Itziar Astigarraga, Nagore Garcia de Andoin, Elixabet Lopez-Lopez, Africa Garcia-Orad, Carmen Lobo, and Idoia Martin-Guerrero
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0301 basic medicine ,Male ,Neurotoxicity Syndrome ,Vincristine ,ATP Binding Cassette Transporter, Subfamily B ,Pharmacogenomic Variants ,Single-nucleotide polymorphism ,Pharmacology ,Polymorphism, Single Nucleotide ,03 medical and health sciences ,0302 clinical medicine ,Pharmacokinetics ,Precursor B-Cell Lymphoblastic Leukemia-Lymphoma ,Genetics ,medicine ,SNP ,Humans ,Child ,Retrospective Studies ,business.industry ,Neurotoxicity ,medicine.disease ,Antineoplastic Agents, Phytogenic ,Multidrug Resistance-Associated Protein 2 ,Pharmacogenomic Testing ,MicroRNAs ,030104 developmental biology ,Haplotypes ,030220 oncology & carcinogenesis ,Pharmacodynamics ,Child, Preschool ,Molecular Medicine ,Female ,Neurotoxicity Syndromes ,Multidrug Resistance-Associated Proteins ,business ,Microtubule-Associated Proteins ,Metabolic Networks and Pathways ,medicine.drug - Abstract
Aim: Vincristine is an important component of acute lymphoblastic leukemia (ALL) treatment protocols that can cause neurotoxicity. Patients treated with LAL/SHOP protocols often suffer from vincristine-related neurotoxicity in early phases of treatment. Recently, a genome-wide association study connected a SNP in CEP72, involved in vincristine pharmacodynamics, with neurotoxicity during later phases of therapy, which was not replicated during induction phase. These results, together with previous studies indicating that polymorphisms in pharmacokinetic genes are associated with drug toxicity, suggest that changes in the activity or levels of vincristine transporters or metabolizers could work as predictors of vincristine-related neurotoxicity in early phases of treatment in pediatric ALL. Patients & methods: We analyzed 150 SNPs in eight key genes involved in vincristine pharmacokinetics and in 13 miRNAs that regulate them. We studied their correlation with neurotoxicity during induction phase in 152 ALL patients treated with LAL/SHOP protocols. Results: The strongest associations with neurotoxicity were observed for two SNPs in ABCC2. The genotypes rs3740066 GG and rs12826 GG were associated with increased neurotoxicity. Conclusion: Polymorphisms in ABCC2 could be novel markers for vincristine-related neurotoxicity in pediatric ALL in early phases.
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- 2016
13. Huge Adrenal Hemorrhagic Endothelial Cyst Secondary to an Adrenal Arteriovenous Malformation and Mimicking a Malignant Lesion
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Isabel Guerra-Merino, Iván Fernández-Vega, and Emma Camacho-Urkaray
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Pathology ,medicine.medical_specialty ,medicine.diagnostic_test ,business.industry ,Endocrinology, Diabetes and Metabolism ,Adrenal Gland Neoplasm ,Arteriovenous malformation ,Magnetic resonance imaging ,General Medicine ,Benign lesion ,medicine.disease ,Pathology and Forensic Medicine ,Endocrinology ,Tomography x ray computed ,Medicine ,Cyst ,business - Published
- 2014
14. Post-mortem findings in Spanish patients with COVID-19; a special focus on superinfections
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Inmaculada Ruiz-Cáceres, Teresa Hermida Romero, Isabel Guerra Merino, Joseba Portu Zapirain, Belén Pérez-Mies, Matilde Sánchez-Conde, Marina Alonso Riaño, Rafael Rubio, Jose Fortés Alen, Ánxela Vidal González, Clara Salas Antón, Elena Múñez, Rafael Sánchez Sánchez, Diana Corona-Mata, Iban Aldecoa Ansorregui, José M. Miró, Raquel Beloqui Pérez de Obanos, Carlos Ibero, Javier Gómez-Román, M. Carmen Fariñas, Teresa Tabuyo Bello, Enrique de Alava, José Miguel Cisneros, Xavier Matías-Guiu, Antonio Rivero, and on behalf of the NECROCOVID Study Group
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COVID-19 ,autopsies ,pathological findings ,infection ,superinfection ,Medicine (General) ,R5-920 - Abstract
IntroductionWhole-body autopsies may be crucial to understand coronavirus disease 2019 (COVID-19) pathophysiology. We aimed to analyze pathological findings in a large series of full-body autopsies, with a special focus on superinfections.MethodsThis was a prospective multicenter study that included 70 COVID-19 autopsies performed between April 2020 and February 2021. Epidemiological, clinical and pathological information was collected using a standardized case report form.ResultsMedian (IQR) age was 70 (range 63.75–74.25) years and 76% of cases were males. Most patients (90%,) had at least one comorbidity prior to COVID-19 diagnosis, with vascular risk factors being the most frequent. Infectious complications were developed by 65.71% of the patients during their follow-up. Mechanical ventilation was required in most patients (75.71%) and was mainly invasive. In multivariate analyses, length of hospital stay and invasive mechanical ventilation were significantly associated with infections (p = 0.036 and p = 0.013, respectively). Necropsy findings revealed diffuse alveolar damage in the lungs, left ventricular hypertrophy in the heart, liver steatosis and pre-infection arteriosclerosis in the heart and kidneys.ConclusionOur study confirms the main necropsy histopathological findings attributed to COVID-19 in a large patient series, while underlining the importance of both comorbid conditions and superinfections in the pathology.
- Published
- 2023
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